ライフサイエンスコーパス: molybdate

1 dc37 in salt-stable complexes independent of molybdate.

2 p90 and Cdc37 independent of the presence of molybdate.

3 e presence of MoeA and low concentrations of molybdate.

4 lation of a blue compound when supplied with molybdate.

5 co in the presence of high concentrations of molybdate.

6 ecursor Z, purified MPT synthase, and sodium molybdate.

7 co) synthesized de novo from precursor Z and molybdate.

8 ar translocation of the AhR, is inhibited by molybdate.

9 perator DNA was 6 times lower than with ModE-molybdate.

10 E(T125I) and ModE(Q216*), were unaffected by molybdate.

11 egion of modA operator DNA in the absence of molybdate.

12 the K(d) increased to 8 nM in the absence of molybdate.

13 n the presence of molybdate and 4 nM without molybdate.

14 ompetitively inhibited growth stimulation by molybdate.

15 plant hsp90 are stabilized by p23 and not by molybdate.

16 of mog, by culture in high concentrations of molybdate.

17 -plant hsp90 complexes are not stabilized by molybdate.

18 odE, which acts as a repressor when bound to molybdate.

19 acile impregnation and calcination of sodium molybdate.

20 sms on CaAl LDHs with increasing loadings of molybdate.

21 led to a transformation of thiomolybdates to molybdate.

22 f the nutrient phosphorus, which reacts with molybdate.

23 .0 +/- 0.2 pM; 50 000-fold more tightly than molybdate.

24 ined from oxidized crystals soaked in sodium molybdate.

25 activity in the absence of externally added molybdate.

26 nity manner with and without the addition of molybdate.

27 modA and modBC was induced by starvation for molybdate.

28 t had no negative effect on higher thiolated molybdates.

29 A high concentration of molybdate (10 microM) supported normal growth of the mod

30 90 (elevated temperature, ATP, Nonidet P-40, molybdate), a five-membered (p23.hsp90.p60.hsp70.hsp40)

31 Tungstate interference of molybdate acquisition by the cell is apparently due in p

32 Lastly, suppressed BNF rates with sodium molybdate additions highlight the prevalence of sulfate

33 MoO4), ferrimolybdite (Fe2(MoO4)3.8H2O), and molybdate adsorbed on ferrihydrite (Fe(OH)3 - MoO4).

34 It was found that ferrimolybdite and molybdate adsorbed on ferrihydrite initially dissolves i

35 n the TMF are powellite, ferrimolybdite, and molybdate adsorbed on ferrihydrite.

36 Molybdate also reduced the amount of free hsp90 present

37 and ModE(T125I) was 3 nM in the presence of molybdate and 4 nM without molybdate.

38 Complex formation is strongly promoted by molybdate and by the nonionic detergent Nonidet P-40.

39 on proteins, we characterized the effects of molybdate and geldanamycin on hsp90 function and structu

40 We used molybdate and monofluorophosphate as inhibitors of sulfa

41 ectrophoresis protocol to examine binding of molybdate and other anions to the ModA periplasmic prote

42 lactate takes up added tungstate rather than molybdate and produces the SCFAs acetate and butyrate, b

43 sphate precipitation reaction using ammonium molybdate and triethylamine under low pH has been applie

44 ile Cj0303 is unable to discriminate between molybdate and tungstate (K(D) values for both ligands of

45 Molybdate and tungstate are strong inhibitors of the pur

46 Thus, molybdate and tungstate bridge the FeZn active site like

47 FS regions shows that the iron sites of both molybdate and tungstate complexes are best simulated by

48 The asymmetric bidentate bridging mode of molybdate and tungstate helps explain the effect of thes

49 y revealed apparent Kd values of binding for molybdate and tungstate of 3 and 7 microM, respectively.

50 te-reducing bacteria, high concentrations of molybdate and tungstate oxyanions inhibit growth, thus r

51 Whereas molybdate and tungstate were bound with high affinity (a

52 actors are associated with genes for various molybdate and tungstate-specific transporting systems as

53 it can be inhibited by low concentrations of molybdate and vanadate.

54 sopoly- and heteropolyvanadates, -niobates, -molybdates and -tungstates aqueous solutions and covers

55 CSP of NaCl, alkali molybdates and V2 O5 with small concentrations of water

56 bohydrate premetabolism, showing how borate, molybdate, and calcium minerals guide the formation of t

57 The enzyme was inhibited by vanadate, molybdate, and EDTA but was resistant to inorganic phosp

58 with the necessary substrates (homocitrate, molybdate, and S-adenosylmethionine [SAM]), demonstratin

59 , Brassica spp. seedlings were supplied with molybdate, and the effects on plant physiology, morpholo

60 e early transition metal oxoanions vanadate, molybdate, and tungstate are widely used inhibitors for

61 Neither tungstate nor vanadate replaced molybdate, and tungstate competitively inhibited growth

62 ntaining various concentrations of selenite, molybdate, and various purine substrates.

63 trong pre-edge absorption from the distorted molybdate anions leaves the oxidation state ambiguous be

64 s; in the absence of steroids, either p23 or molybdate are also required as reported previously.

65 ion step, where adenylated MPT (MPT-AMP) and molybdate are the substrates.

66 oxoanion analogues orthovanadate, tungstate, molybdate, arsenate, and sulfate were shown to inhibit t

67 ModE-molybdate at 5 nM completely protected the modABCD opera

68 m Azotobacter vinelandii First, we show that molybdate, ATP, and Mg(2+) consecutively bind into the o

69 a conformational change of the protein upon molybdate binding.

70 nsive conformational changes not only in the molybdate-binding domain, but also in the DNA-binding do

71 part of the fused gene lacked a recognizable molybdate-binding domain.

72 The X-ray structures of the cytoplasmic molybdate-binding protein ModG from Azotobacter vineland

73 The C-terminal domain contains the molybdate-binding site and residues implicated in bindin

74 E dimer allows the probable locations of the molybdate-binding sites of the latter to be assigned.

75 n to the oxidized form, the structure of the molybdate-bound form of the protein was determined from

76 should improve DNA binding of the activated molybdate-bound ModE.

77 ons, the nifH1 mutant grew in the absence of molybdate but not in its presence, using VnfH, while the

78 hosphatases (sodium orthovanadate and sodium molybdate) but not by inhibitors of serine/threonine pho

79 ctably transport low concentrations of 99Mo (molybdate), but did transport high concentrations.

80 es molybdenum incorporation at low levels of molybdate, but MogA has an alternative function, possibl

81 OsMOT1;1 exhibits transport activity for molybdate, but not sulfate, when heterogeneously express

82 The ABC transporter, MolBC-A, imports molybdate by passing substrate from the binding protein

83 amino acids is essential for the binding of molybdate by the ModE protein.

84 We show how this fate might be avoided using molybdate-catalyzed rearrangement of a branched pentose

85 the Hsp90 inhibitory agents geldanamycin and molybdate, Cdk2 is shown to be a genuine client of the H

86 The conversion of MPT into Moco by molybdate chelation apparently occurs concomitantly with

87 In the present work, a cobalt molybdate (CoMoO(4)) hollow spheres-incorporated graphit

88 s or with the tyrosine phosphatase inhibitor molybdate completely blocked stimulation of HCO(3)(-) ab

89 ly mimic in its conformation the native ModE-molybdate complex, which binds to a DNA sequence motif o

90 ahedral geometry was observed for the weaker molybdate complex.

91 om Escherichia coli functions as a sensor of molybdate concentration and a regulator for transcriptio

92 In periods of high extracellular molybdate concentration, H. influenzae makes use of para

93 ene product in response to the intracellular molybdate concentration.

94 This phenotype was rescued by increasing molybdate concentrations in the nutritive solution, or u

95 The combined release of protons and molybdate consistently results in a sample pH < 2 as wel

96 experiments with pure cytochrome showed that molybdate could oxidize the reduced cytochrome, although

97 H1, which was also made in cells starved for molybdate, could substitute for VnfH.

98 tely 80%) in settling particles amended with molybdate, demonstrating the prominent role of biologica

99 sm by binding to specific DNA sequences in a molybdate-dependent fashion.

100 ansporter, is repressed by ModE in vivo in a molybdate-dependent fashion.

101 ption factor to help coordinate nitrate- and molybdate-dependent napF expression by the Fnr, NarP, Na

102 s, we confirmed that IHF plays a role in the molybdate-dependent regulation of dmsA-lacZ expression i

103 The molybdate-dependent transcriptional regulator (ModE) fro

104 Both p23 and molybdate enhance and stabilize the nucleotide-bound sta

105 h the reaction of Si with acidified ammonium molybdate, followed by precipitation with triethylamine

106 a dense epitaxial hydroxide layer on nickel molybdate, forming an effective protective barrier to pr

107 ple pH < 2 as well as a sufficient excess of molybdate, fulfilling the conditions required for the st

108 Borate, sulfate, phosphate, and molybdate had essentially no effect on Apo activation an

109 Molybdate has the same ability as p23 to stabilize GR he

110 anscriptional factors controls tungstate and molybdate homeostasis in sulfate-reducing deltaproteobac

111 ires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabo

112 We also establish that only molybdate, homocitrate, MgATP, and Fe protein are essent

113 se genes, the vupABC genes were repressed by molybdate; however, unlike the V-nitrogenase genes the v

114 High-affinity transport of molybdate in A. variabilis was mediated by an ABC-type t

115 ionally synthesized by chemical reduction of molybdate in aqueous solutions, generating complex nanos

116 controlling the homeostasis of tungstate and molybdate in sulfate-reducing deltaproteobacteria.

117 he formation of a complex of As(V) ions with molybdate in the presence of 50.0 mmol L(-1) sulfuric ac

118 e activation/reduction pathway combines with molybdate in the production of activated molybdenum.

119 e hydrogen sulfide is known to interact with molybdate in the production of thiomolybdate, it is poss

120 After the inclusion of sodium molybdate in the reconstitution mixture, active sulfite

121 us oxyanions tested, only tungstate replaced molybdate in the repression of modA by ModE, but the aff

122 tion constant (Kd) of 45 nM, and addition of molybdate, in physiologically relevant amounts, signific

123 to the moaA promoter appeared to be largely molybdate independent both in vitro and in vivo.

124 The molybdate-independent mutant ModE proteins apparently mi

125 When supplied with (colorless) molybdate Indian mustard (Brassica juncea) seedlings acc

126 t-accumulated Mo was different than that for molybdate, indicating complexation with a plant molecule

127 cin prevented hsp90 from assuming natural or molybdate-induced conformations that allow salt-stable i

128 While molybdate inhibited As biomethylation, this effect was u

129 Molybdate inhibited Hg methylation more efficiently in t

130 Molybdate inhibited hsp90-mediated p56lck biogenesis and

131 showing significant sulfate stimulation and molybdate inhibition of methylation in SR sediments.

132 In this study, we demonstrate that molybdate inhibits AhR gene activation in both HepG2 and

133 However, neither the mechanism of molybdate insertion into MPT nor the structure of Moco p

134 ctural information to deduce a mechanism for molybdate insertion into MPT-AMP.

135 hich MtMOT1.3 is responsible for introducing molybdate into nodule cells, which is later used to synt

136 otoxicity experiments on MoS2 nanosheets and molybdate ion controls reveal the relative roles of the

137 The location of the molybdate ion near heme IV in the crystal structure sugg

138 he MBM is that arsenate also reacts with the molybdate ion to produce the same peak in the visible sp

139 electrocatalyst, in which the in situ-formed molybdate ions on its surface repel chloride ions.

140 of the reaction between orthophosphates and molybdates ions where ascorbic acid and antimony potassi

141 as is nitrate reductase; thus, transport of molybdate is important for growth of this strain.

142 e current data, the released and accelerated molybdate is pressed through the cage wall, presumably b

143 odels, the nature of chemical bonding in the molybdates is explained by molecular orbital theory and

144 also a Type II importer, but its substrate, molybdate, is approximately 10-fold smaller than vitamin

145 ression when the essential enzyme component, molybdate, is limiting in the cell environment.

146 energy into kinetic energy via an activating molybdate kinase and an exothermic pyrophosphatase react

147 AmBO(3), borate halides Ca(5)Am(BO(3))(4)Cl, molybdates Li(0.5)Am(0.5)MoO(4), and fluorides CsAm(2)F(

148 uptake and translocation of molybdate under molybdate-limited condition.

149 nct effect on the conformation of Hsp90, and molybdate lowered the concentration of novobiocin requir

150 as luminescence, of rare earth tungstate and molybdate materials.

151 for vanillin detection, utilizing manganese molybdate (MnMoO(4)) nanoparticles integrated with funct

152 n in a defined culture medium, but only when molybdate (Mo) and selenite (Se) were also added.

153 303 and Cj1538-1540) homologous to bacterial molybdate (ModABC) uptake systems and the tungstate tran

154 the larger membrane-spanning subunits of the molybdate (ModBC) and maltose (MalFGK) ABC transporters.

155 a trimer capable of binding a total of eight molybdate molecules that are distributed between two dis

156 The soluble form of Mo, molybdate (MoO(4) (2-) ), is incorporated into and adsor

157 seawater and sediment should be observed if molybdate (MoO4(2-)) is quantitatively transformed to te

158 nts and X-ray techniques was used to examine molybdate (MoO4(2-)) sorption mechanisms on CaAl LDHs wi

159 The use of rare earth tungstate and molybdate nano- and micromaterials as single materials f

160 lectrocatalytic activity of structured metal molybdate nanomaterials, MMoO(4) (M = Fe, Ni, and Zn), o

161 designing of porous 3D flower-like neodymium molybdate nanosheets (pf-NdM NSs) and (ii) attaining rea

162 three distinct regions of protection by ModE-molybdate on the modA operator/promoter DNA, GTTATATT (-

163 fects of sulphate-mimetics (like chromate or molybdate) on sulphate transport.

164 Next, we propose a nucleophilic attack of molybdate onto the gamma-phosphate of beta-ATP, analogou

165 s ATP-induced state is achieved by including molybdate or by use of the ATP analogue ATPgammaS.

166 wo cultures, Hg methylation assays following molybdate or chloroform inhibition (a specific inhibitor

167 When molybdate or p23 is also present during the incubation w

168 r dichroism spectroscopy indicated that when molybdate or tungstate bind to ModE there is little chan

169 Addition of molybdate or tungstate to the protein results in almost

170 were expressed in the presence or absence of molybdate or vanadate.

171 id not grow diazotrophically with or without molybdate or vanadate.

172 pendent process that is stimulated by sodium molybdate, other group VI-A transition metal oxyanions,

173 t 2.22 A, the latter being associated with a molybdate oxygen.

174 demonstrated that snr-1 was not regulated by molybdate, oxygen, or nitrate.

175 of this interaction was found to be two ModE-molybdate per modA operator DNA.

176 he propene oxidation, while the K-doped iron molybdate pools electrons to activate dioxygen.

177 e sensitivity of the combined pyrogallol red-molybdate precipitation/SDS-PAGE procedure is approximat

178 phase period when partial Mo(VI) centers of molybdate precursors are reduced to form {Mo(V)2(acetate

179 ssie Brilliant Blue (CBB) and Pyrogallol Red-molybdate (PRM) protein dye-binding assays to interferen

180 ch repressed modA-lac even in the absence of molybdate, protected the same region of modA operator DN

181 In summary, the pyrogallol red-molybdate protein precipitation procedure facilitates th

182 ggest a unique mechanism for the ATP-powered molybdate pumping process based on X-ray crystallography

183 However, in the absence of p23 or molybdate, rapid disassembly of GR.hsp90 complexes appar

184 The factors affecting the molybdate reactive P (MRP) in these waters were analyzed

185 The stabilization was monitored by measuring molybdate-reactive silica and also by a combination of l

186 ined physical (centrifugation) and chemical (molybdate reactiveness) fractionation of leached P showe

187 DRP) in ice cores has been developed using a molybdate reagent and a 2-m liquid waveguide capillary c

188 fectively precipitated with a pyrogallol red-molybdate reagent from commonly used chromatographic buf

189 ModE, in the presence of 1 mM molybdate, repressed the production of plasmid-encoded M

190 gene, ModE, is a member of a unique class of molybdate-responsive DNA binding proteins.

191 nd conferred a modE phenotype (i.e., loss of molybdate-responsive gene expression) in vivo.

192 sion is impaired in strain defective for the molybdate-responsive ModE transcription factor.

193 regulators are similar to those of the known molybdate-responsive regulator ModE, while their DNA-bin

194 To determine if the molybdate-responsive transcription factor ModE is involv

195 Molybdate, selenate, chromate ("chromium VI"), arsenate,

196 quantitative extraction of (99m)Tc from the molybdate solution and complete release of (99m)Tc after

197 f these key species in the reduced acidified molybdate solutions and to observe the templation of the

198 y effective at separating (99m)TcO4 (-) from molybdate solutions.

199 aldoses, i.e., various molybdenum oxide and molybdate species, nickel(II) diamine complexes, alkali-

200 at promotion is most effective for dispersed molybdate species, with a decline at higher Mo loadings.

201 The modABC gene products constitute the molybdate-specific transport system in Escherichia coli.

202 peron in Escherichia coli, which codes for a molybdate-specific transporter, is repressed by ModE in

203 usly in euxinic marine waters after adding a molybdate spike and occur naturally in sulfidic geotherm

204 thelia followed by staining with an ammonium molybdate spray reagent revealed three phospholipid comp

205 nal nitric-oxide synthase (nNOS) exists as a molybdate-stabilized nNOS.hsp90 heterocomplex in the cyt

206 Because sodium molybdate stabilizes the AhR-HSP90 interaction and inhib

207 odABCD transcription even in the presence of molybdate, suggesting that ModE is a repressor.

208 and ModBC-A) to take up a greater amount of molybdate than a strain with ModBC-A alone.

209 tained by the reaction between phosphate and molybdate that is consequently reduced at the electrode

210 This Pi forms a complex with ammonium molybdate that is then reduced by ascorbic acid to provi

211 ichaelis constants for MgATP and sulfate (or molybdate), the dissociation constant of E.APS, and the

212 c gas-phase conversion of n-decane over iron molybdate, the product distribution correlates with the

213 ns 1 and 2 are essential for binding of ModE-molybdate to DNA, whereas the protected region 3 increas

214 the principle that phosphate ions react with molybdate to form redox molybdophosphate precipitates on

215 phosphate, the latter reacting with ammonium molybdate to form the ammonium phosphomolybdate complex

216 The binding of molybdate to ModE resulted in a decrease in fluorescence

217 Addition of molybdate to purified B. rapa anthocyanin resulted in an

218 winging door: allowing just enough space for molybdate to slip into the cell.

219 bstrate uptake studies, we reveal details of molybdate transport and the logistics of uptake systems

220 MtMOT1.3 shows molybdate transport capabilities when expressed in yeast

221 These results show that molybdate transport in E. coli is regulated by ModE, whi

222 se, indicating that there was a low-affinity molybdate transport system in this strain.

223 rt system might also serve as a low-affinity molybdate transport system in this strain.

224 tration, H. influenzae makes use of parallel molybdate transport systems (MolBC-A and ModBC-A) to tak

225 Members of the molybdate transporter family Molybdate Transporter type

226 und that MolBC-A functions as a low affinity molybdate transporter in its native environment.

227 Members of the molybdate transporter family Molybdate Transporter type 1 (MOT1) were identified in t

228 The Escherichia coli molybdate transporter, encoded by the modABCD operon, is

229 ABCD operon, which encodes the high-affinity molybdate transporter, is mediated by the ModE protein.

230 We identified a molybdate transporter, OsMOT1;1, as the causal gene for

231 or the permease component of a high-affinity molybdate transporter.

232 RNA motif located upstream of genes encoding molybdate transporters, molybdenum cofactor (Moco) biosy

233 has to be provided by the host plant through molybdate transporters.

234 Molybdate treatment decreased methylation rate potential

235 Because high levels of molybdate, tungstate, and selenite restored growth to wi

236 mporters of identical substrate specificity (molybdate/tungstate), and find that their interactions w

237 involved in the uptake and translocation of molybdate under molybdate-limited condition.

238 ve in the modA gene were unable to transport molybdate unless high levels of the anion were supplied

239 gene product is essential for high affinity molybdate uptake by the cell.

240 estingly, modC strains that are defective in molybdate uptake exhibit impaired anaerobic induction an

241 -electron paramagnetic resonance and in vivo molybdate uptake studies were used to test the impact of

242 es the protein components of a high affinity molybdate uptake system.

243 polyaniline to lower the pH, the delivery of molybdate via a molybdenum electrode, and the formation

244 ction between the modA operator DNA and ModE-molybdate was 0.3 nM, and the K(d) increased to 8 nM in

245 H and XDH, increased when either selenite or molybdate was added to the culture medium.

246 on benzoate, was retarded unless 0.1 microM molybdate was added to the medium.

247 Molybdate was as effective in inhibiting Hg methylation

248 The Hsp90 antagonist molybdate was necessary to stabilize the interactions be

249 5 complexed with the inhibitors vanadate and molybdate were solved at 2.2 A resolution and compared t

250 nds are the first examples of charge-neutral molybdate wheels as confirmed by BVS, solubility experim

251 (>2 mM) negatively affected the detection of molybdate, which eluted mainly in the dead volume, but h

252 ugh the formation of a complex with ammonium molybdate, which has an absorbance maximum at 700 nm.

253 modABCD transcription even in the absence of molybdate, which implies that this stretch of amino acid

254 et, other xenobiotics that are sulfated, and molybdate, which inhibits sulfate intestinal absorption,

255 e presence of hsp90 because it is blocked by molybdate, which prevents hsp90 dissociation.

256 ure of Escherichia coli ModE in complex with molybdate, which was determined at 2.75A from a merohedr

257 K, vnfH was expressed only in the absence of molybdate, with or without vanadate.

258 umber of steroid receptors, we reasoned that molybdate would be a useful tool in delineating the role