ライフサイエンスコーパス: monomethylation

1 on of MLL3, and in turn, histone H3 lysine 4 monomethylation.

2 -l-methionine (SAM) for SET8-catalyzed H4K20 monomethylation.

3 36 along with a reduced preference for H3K36 monomethylation.

4 one SET domain catalyzes at least weak H3K4 monomethylation.

5 r its activity, and enhances PGC-1alpha K224 monomethylation.

6 gle type III PRMT solely capable of arginine monomethylation.

7 st-translational modification through lysine monomethylation.

8 In contrast, H3K27 monomethylation, a modification enriched at pericentrome

9 We identify H4K20 monomethylation, a modification previously linked with r

10 lian COMPASS-like proteins deficient in H3K4 monomethylation activity and point to a possible role fo

11 thermore, we observed that WDR5 inhibits the monomethylation activity of the MLL3 core complex, which

12 complexes, and its expression reduces G3BP1 monomethylation and asymmetric dimethylation at three Ar

13 ginine methyltransferase 6 (PRMT6) catalyzes monomethylation and asymmetric dimethylation of arginine

14 These sites show high levels of histone H3K4 monomethylation and CBP/P300 binding characteristic of e

15 ied five putative enhancers marked with H3K4 monomethylation and depletion of H3.

16 catalytic SET domain of MLL1 (known for H3K4 monomethylation and dimethylation) remains unclear.

17 genome-wide analysis of its substrates H3K4 monomethylation and dimethylation, and gene expression p

18 Cmi is necessary for robust H3K4 monomethylation and H3K27 acetylation that mark active e

19 ancer elements marked by histone H3 lysine 4 monomethylation and histone H3 lysine 27 acetylation, de

20 and displays a broad scope (69 examples) for monomethylation and hydrogenation of unsaturated chalcon

21 cupied DNA segments have high levels of H3K4 monomethylation and low levels of H3K27me3 around the ca

22 Our studies identify the importance of H4K20 monomethylation and of PR-SET7 for L3MBTL1 function.

23 methyl peptides to globally profile arginine monomethylation and symmetric dimethylation after PRMT5

24 Monomethylation and trimethylation of histone H4-lysine

25 ges had increased histone 3, lysine 4 (H3K4) monomethylation, and H3K9 acetylation, accompanied by de

26 lysine 4 trimethylation, histone H3 lysine 4 monomethylation, and histone H3 lysine 27 acetylation (H

27 ites also correspond to high levels of H3K27 monomethylation, and mutation of the catalytic SET domai

28 with histone H4-Y72 phosphorylation, H4-K20 monomethylation, and the Ki-67 proliferation marker.

29 -translational modification, possibly lysine monomethylation, appears to be the single most important

30 Although levels of H3.3K79 monomethylation are higher than that of H3.2/H3.1, the r

31 methyl-lysine modifications, H4K20 and H3K27 monomethylation are versatile and dynamic with respect t

32 lation is reduced dramatically compared with monomethylation as the concentration of the substrate is

33 Most interestingly, monomethylation at Arg-17 in histone H4 not only raised

34 Rps3 is stoichiometrically modified by omega-monomethylation at arginine-146 by mass spectrometric an

35 se of H3K27 acetylation and increase of H3K4 monomethylation at derepressed Alus, making them resembl

36 e1 in these cells, with the greatest loss of monomethylation at enhancer regions.

37 We also observed enrichment of H3K79 monomethylation at intergenic regions occupied by DNA-bi

38 ates nucleosome remodeling, followed by H3K4 monomethylation at large numbers of genomic regions asso

39 In contrast, dimethylation at Lys-27 and monomethylation at Lys-36 were commonly found together.

40 proteomic analysis, this study revealed AF-1 monomethylation at Lys-464.

41 Furthermore, we show that RB monomethylation at lysine 860 provides a direct binding

42 we show that the enzyme primarily generates monomethylation at this position.

43 dentify a glutamate residue critical for its monomethylation behavior.

44 es of the BAF component BAF45C indicate that monomethylation, but not trimethylation, is accommodated

45 e H3 (H3K4), whereas enhancers are marked by monomethylation, but not trimethylation, of H3K4.

46 anism of NF-kappaB regulation through lysine monomethylation by SET9 methyltransferase.

47 ghest catalytic efficiency (k(cat)/K(M)) for monomethylation compared to di- and trimethylation on H3

48 repressive complex 2 (PRC2), which mediates monomethylation, dimethylation, and trimethylation of hi

49 Complex 2 (PRC2), the enzyme that catalyzes monomethylation, dimethylation, and trimethylation of ly

50 Arginines were modified by monomethylation, dimethylation, or deimination.

51 t with its role in the maintenance of Lys-20 monomethylation during cell division.

52 irrors the progressive accumulation of H4K20 monomethylation during the cell cycle.

53 c2, resulting in the simultaneous removal of monomethylation from H3K4 and acetylation from H3K27, re

54 companied by a decrease in levels of histone monomethylation (H3-K4me1).

55 e modifications, including histone H3 Lys 27 monomethylation (H3K27me1), imparted by ATXR5 and ATXR6.

56 lls showed a deficiency in histone H3 Lys 27 monomethylation (H3K27me1), while DeltaEZL2 cells, delet

57 SR1 and the epigenome of histone H3 lysine 4 monomethylation (H3K4me1) in a cancer- and cell type-spe

58 Histone H3 lysine 4 monomethylation (H3K4me1) is an evolutionarily conserved

59 ecifically recognizes the histone 3 lysine 4 monomethylation (H3K4me1) mark.

60 ndreds of mouse-specific histone H3 lysine 4 monomethylation (H3K4me1)- and histone H3 lysine 27 acet

61 ed the expression of MLL1 and menin and H3K4 monomethylation (H3K4me1); MLL1 and menin were expressed

62 We now report the presence of H3K56 monomethylation (H3K56me1) in mammalian cells and find t

63 K79 dimethylation (H3K79me2) is converted to monomethylation (H3K79me1) at HOX loci as hematopoietic

64 e, prometaphase, and metaphase, whereas H3K9 monomethylation (H3K9me1) and dimethylation (H3K9me2), b

65 We found that H4K20 monomethylation (H4K20me1) is enriched at centromeres.

66 tion (H4K16ac) is underrepresented and H4K20 monomethylation (H4K20me1) is enriched on hermaphrodite

67 monomethyltransferase responsible for H4K20 monomethylation (H4K20me1) that is the substrate for fur

68 cetylation (H4K16ac) and enrichment of H4K20 monomethylation (H4K20me1)) in both wild type and develo

69 f BAP1 results in a marked decrease in H4K20 monomethylation (H4K20me1).

70 igenetic enhancer marks (histone H3 lysine 4 monomethylation, histone H3 lysine 27 acetylation, and t

71 Here, we find that SET7/9 monomethylation in a putative nucleolar localization reg

72 Our findings point to a unique role for H3K4 monomethylation in establishing boundaries that restrict

73 ur at weak/poised enhancers marked with H3K4 monomethylation in hematopoietic progenitor cells.

74 sults demonstrate the critical role of H4K20 monomethylation in mammals in a developmental context.

75 1) and Mll4 (GeneID 381022) in enhancer H3K4 monomethylation in mouse embryonic fibroblast (MEF) cell

76 f ATXR5/ATXR6-catalyzed histone H3 lysine 27 monomethylation in plants depends on H3.1, TSK, and DNA

77 H3K27 acetylation, H3K4 trimethylation, H3K4 monomethylation), indicating that the binding sites were

78 Conversion of H3K79 monomethylation into di- and trimethylation correlated w

79 These data strongly suggest that H4 monomethylation is a key triggering point in PARP-1 depe

80 In this report, we demonstrate that H3K9 monomethylation is dependent upon the PR-Set7 H4K20 mono

81 motifs, KX(G/A/V/I)N and KT(I/L/F), whereas monomethylation is pervasive throughout OmpB.

82 We find that, in vitro, LSD1 removes both monomethylation (K370me1) and dimethylation (K370me2) at

83 Lysine monomethylation (Kme) is an impactful post-translational

84 is required to preserve histone H3 lysine 4 monomethylation levels and to recruit the histone methyl

85 ase activity results in reduced histone H3K4 monomethylation levels, followed by a global increase in

86 x, results in increased H3K4me1 (H3 lysine 4 monomethylation) levels.

87 rase 7 (PRMT7) catalyzes the introduction of monomethylation marks at the arginine residues of substr

88 Monomethylation-mediated repression was not restricted t

89 coded by TMT1 catalyzes the AdoMet-dependent monomethylation of 3-isopropylmalate, an intermediate of

90 rylation on Tyr109 [p-Tyr109], p-Ser111, and monomethylation of Arg114 [me-Arg114]) within an N-termi

91 Monomethylation of dimethylmalonate followed by alkylati

92 biquitylation of H2B-K123 is dispensable for monomethylation of H3-K4 and H3-K79 but is required for

93 tment of these factors as well as diminished monomethylation of H3K4 (H3K4me1) and acetylation of H3K

94 modelers and histone modifiers including the monomethylation of H3K4 (H3K4me1) by MLL3 (KMT2C) and ML

95 Monomethylation of H4K20 (H4K20me1) is mediated by the c

96 Set8/KMT5a is the sole enzyme that catalyzes monomethylation of H4K20 (H4K20me1).

97 Mutating pr-set7 disrupts monomethylation of H4K20 along heat shock loci and aboli

98 Monomethylation of H4K20 has been implicated in regulati

99 ila genome, suggesting that PR-Set7-mediated monomethylation of H4K20 is critical for maintaining the

100 tablishment of the epigenetic mark H4K20me1 (monomethylation of H4K20) by PR-Set7 during G2/M directl

101 n greater abundance, as well as a pattern of monomethylation of histone H3 at lysine 4 (H3K4) across

102 Monomethylation of histone H3 at lysine 4 (H3K4me1) and

103 ed tissue-specific enhancer marks, including monomethylation of histone H3 at lysine 4 (H3K4me1) and

104 e or primed enhancers are commonly marked by monomethylation of histone H3 at lysine 4 (H3K4me1) in a

105 sence of chromatin regulators p300 and BRG1, monomethylation of histone H3 at lysine 4 (H3K4me1), and

106 nd that the subset of enhancers enriched for monomethylation of histone H3 Lys4 (H3K4me1) and binding

107 Monomethylation of histone H3 lysine 27 (K27me1) is a po

108 Enrichment of monomethylation of histone H3 lysine 4 (H3K4me1) is a ma

109 Monomethylation of histone H3 on Lys 4 (H3K4me1) and ace

110 lated recruitment of Stat5b, as did lysine 4 monomethylation of histone H3, which was enriched in 6/7

111 Monomethylation of histone H3K4 (H3K4me1) is relatively

112 We present evidence that PRMT7-mediated monomethylation of histone H4 Arg-17 regulates PRMT5 act

113 -Set7/Set8 is the sole enzyme that catalyzes monomethylation of histone H4 at K20 (H4K20me1).

114 , a histone methyltransferase that catalyzes monomethylation of histone H4 at lysine 20 (H4K20me1), i

115 8/KMT5A is the sole enzyme known to catalyze monomethylation of histone H4 lysine 20 (H4K20) and is p

116 its corresponding histone modification, the monomethylation of histone H4 lysine 20 (H4K20), display

117 only known methyltransferase that catalyzes monomethylation of histone H4 lysine 20 (H4K20).

118 During G2 and mitosis, Set8 catalyzes monomethylation of histone H4 on lysine 20 (H4K20me1), w

119 Mitochondrial KMT9 regulates PDC activity by monomethylation of its subunit dihydrolipoamide transace

120 KMT5A specifically recruited monomethylation of Lys20 of histone H4 (H4K20me) to the

121 Monomethylation of lysine 20 of histone H4 (H4K20me1) is

122 dothelial cells, silencing of Set7 prevented monomethylation of lysine 4 of histone 3 and abolished N

123 Patients with T2DM showed Set7-dependent monomethylation of lysine 4 of histone 3 on NF-kB p65 pr

124 rase (KMT2A) resulting in increased promoter monomethylation of lysine 4 of histone 3.

125 Monomethylation of lysine 4 on histone H3 (H3K4me1) is a

126 We previously found that monomethylation of p53 at lysine 382 (p53K382me1) by the

127 ls as measured by reduction of SMYD2-induced monomethylation of p53 Lys(370) at submicromolar concent

128 These results suggest that monomethylation of PR at Lys-464 probably has a repressi

129 Remarkably, we find that MLL3/4 provokes monomethylation of promoter regions and the conditional

130 ions of assembled TDP-43, citrullination and monomethylation of R293, and indicate that they may faci

131 ewhat surprisingly, AtMETTL20 also catalyzed monomethylation of RpL7/L12 on Lys-86, a common modifica

132 lysine methyltransferase that catalyzes the monomethylation of several protein substrates including

133 This unique feature, in combination with the monomethylation of the phosphonic acid, renders DHP a Tr

134 Monomethylation of the potentially ambident RNH[N(O)NO](

135 are the specific enzymes responsible for the monomethylation of the two sites in Rpl42ab.

136 Monomethylations of cytidine and adenosine are common po

137 The monomethylations of H3K27, H3K9, H4K20, H3K79, and H2BK5

138 egulation of di- and trimethylation, but not monomethylation, of H3-Lys-4 in vivo.

139 Our in vitro studies show that the onset of monomethylation on an unmethylated histone H3 by COMPASS

140 family of epigenetic enzymes responsible for monomethylation or dimethylation of arginine residues on

141 suggesting that catalytic steps dictate the monomethylation preferences of EHMT1, EHMT2, and SUV39H1

142 ng positional variables, the requirement for monomethylation (primary and dialkylated amides are inac

143 yl cyanide, complex 9 is regenerated and the monomethylation product 2-phenylpropanenitrile is releas

144 alytic efficiency (k(cat)/K) for the zero to monomethylation reaction of H3K27 and diminished efficie

145 The favored monomethylation reaction was also reflected in k(cat) va

146 f p300 recruitment, while MLL4-mediated H3K4 monomethylation, reciprocally, requires p300 function.

147 ncurrently to yield N-methylamines with high monomethylation selectivity via the overall transfer of

148 370, a previously identified Smyd2-dependent monomethylation site.

149 , each synthesized in-house via a stepwise N-monomethylation synthesis strategy using readily availab

150 n reduces heat shock protein 70 (HSP70) R469 monomethylation to disrupt HSP70-HSF1 repressive complex

151 ver, the molecular mechanism linking p53K382 monomethylation to repression is not known.

152 -K79 but is required for the transition from monomethylation to subsequent methylation states.

153 ration and identification of tails involving monomethylation, trimethylation, acetylation, or phospho

154 At some locations, H3K27 monomethylation was also found to be associated with chr

155 read 3' into the bodies of genes, while H3K4 monomethylation was diminished.

156 and trimethylation, while histone 3 lysine 4 monomethylation was not affected.

157 ATXR6-dependent histone H3 lysine 27 (H3K27) monomethylation, we show that millions of base pairs of

158 Selective monoreduction and monomethylation were observed for 3.

159 The sequence is halted at monomethylation when the conformation of the Enz.Lys-N(M

160 PKMT1 catalyzes predominantly monomethylation, whereas PKMT2 catalyzes mainly trimethy

161 This study revealed that PGC-1alpha monomethylation, which is dependent on oxygen availabili

162 di-OH-tam) catechol, was demonstrated by its monomethylation with [3H]S-adenosyl-L-methionine ([3H]SA

163 s9 dimethylation and a significant amount of monomethylation within silent euchromatin.

164 that the WRAD subcomplex catalyzes weak H3K4 monomethylation within the context of the MLL3 core comp

165 ones display markedly reduced levels of H3K4 monomethylation without obvious changes in the levels of

166 und that CTK1 deletion nearly abolished H3K4 monomethylation yet caused a significant increase in H3K