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1 ensity centrifugation (to recover intact 70S monosomes).
2 ubunit of the mitochondrial ribosome and the monosome.
3 SU and consequently reduced formation of the monosome.
4 03 x 10(-7) cm(2) s(-1) for the 70 S E. coli monosome.
5 ing to ensure the assembly of the mature 55S monosome.
6 cles and complete conversion of polysomes to monosomes.
7 omes exist in two populations: polysomes and monosomes.
8 NA from polyribosome-associated polysomes to monosomes.
9 ors, ribosomal large and small subunits, and monosomes.
10 uses an accumulation of 60S subunits and 80S monosomes.
11 nd c-myc transcripts from heavy polysomes to monosomes.
12 ts with 60 S ribosomal subunits but not with monosomes.
13 accompanied by the transient increase in 70S monosomes.
14 se gradients used to separate polysomes from monosomes.
15 tes that are incapable of forming functional monosomes.
16 ; and (4) mRNAs associated specifically with monosomes.
17    FAM86A depletion in LUAD cells causes 80S monosome accumulation and mRNA translation inhibition.
18  determine the translational activity of 80S monosomes across different tissues in Drosophila melanog
19 ned their potency at converting polysomes to monosomes across other commonly used model organisms, in
20 eads to severely decreased levels of the 55S monosome and attenuated mitochondrial protein synthesis.
21 f MPV17L2 results in marked decreases in the monosome and both subunits of the mitochondrial ribosome
22 no substantial increase in the size of their monosome and polysome peaks, suggesting that similar num
23 anslation was examined by RT-PCR analysis of monosome and polysome sucrose gradient fractions from My
24 was fractionated into pools of polysomes and monosomes and a ribosome-free pool.
25           RPG knockdowns decreased miRNAs in monosomes and increased their target mRNAs in polysomes.
26 sively, to what extent translation occurs on monosomes and its importance for overall translational o
27     Finally, the levels 40 S, 60 S, and 80 S monosomes and polyribosomes are unaffected by the loss o
28 ing revealed that ORF57 associates with both monosomes and polysomes and that its association with po
29                           miRNA profiling of monosomes and polysomes demonstrated that miRNAs cosedim
30  neurons, revealing a dynamic switch between monosomes and polysomes in neuronal translation.
31  40S and 60S ribosomal subunits but also 80S monosomes and polysomes in the remaining kidney.
32 from 9.8-fold to 6.0-fold while flux between monosomes and polysomes remained constant.
33 and beta-glucan, and reduced levels of total monosomes and polysomes were observed.
34  affect total c-jun mRNA or its flux between monosomes and polysomes.
35 dberg ribosomal subunits, intact 80-Svedberg monosomes and polysomes.
36 ted the polysome profile in the direction of monosomes and ribosomal subunits.
37       Hyperphosphorylated 9G8 was present in monosomes and small polyribosomes, whereas soluble fract
38 by a global shift in mRNAs from polysomes to monosomes and the downregulation of genes involved in tr
39  observations, sucrose gradient purified 80S monosomes and translating polysomes each contained TbRAC
40 PA), and c-jun mRNA was quantified in total, monosome, and polysome fractions by real-time polymerase
41 nits to create the translationally competent monosome, and provide evidence that assembly of the smal
42                     RNA-Seq analysis of head monosome- and polysome-translated mRNAs, revealed that h
43       We found that the vast majority of 80S monosomes are elongating, not initiating.
44 We discovered that while head and embryo 80S monosomes are highly translationally active, testis and
45 translationally active, testis and ovary 80S monosomes are translationally inactive.
46  mRNAs that are specifically associated with monosomes are translationally up-regulated during seed g
47  all polysomal ribosomes in a stalled queue, monosomes assembled in RRL, in vitro reconstituted 80S e
48 he latter group includes MTERF4, involved in monosome assembly, and MRM2, the methyltransferase that
49 the small mitoribosomal subunit and impaired monosome assembly.
50 iling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells
51 ndances of very stable mRNAs, an increase in monosomes at the expense of large polysomes, and appeara
52 tion of BipA-H78A causes accumulation of 70S monosomes at the expense of polysomes, suggesting that t
53                Dissociation of polysomes and monosomes both involved ribosomal splitting, enabling Li
54 e less efficient at mediating bypassing than monosomes, both in vitro and in vivo, due to their preve
55 oribosomal subunits and the formation of the monosome by establishing quality-control checkpoints dur
56 bunit and polysome content and decreased 80S monosome content.
57              These PTC-containing mRNAs were monosome-enriched and rarely contributed to expression o
58         Our data highlight the importance of monosomes for the translation of highly regulated mRNAs.
59 ion of several mt-LSU proteins and decreased monosome formation.
60 gulatory proteins tend to be enriched in the monosome fraction.
61 au1-dependent, being mainly localized in the monosome fractions when Stau1 is downregulated and exclu
62 nsional reconstruction of the eukaryotic 80S monosome from a frozen-hydrated electron microscopic pre
63 ity while thoroughly converting polysomes to monosomes in all examined species.
64 e (39S) subunit of the ribosome from the 55S monosomes in an active process.
65 d that most stored mRNAs are associated with monosomes in dry seeds; therefore, we focus on monosomes
66 and protein synthesis is mostly performed by monosomes in head and embryo, while polysomes are the ma
67 g to examine the translational status of 80S monosomes in Saccharomyces cerevisiae.
68         Loss of polysomes with increased 80S monosomes in the polyamine-depleted cells suggests a dir
69 nosomes in dry seeds; therefore, we focus on monosomes in this study.
70 somal subunits relative to the amount of 70S monosomes increase in Era-depleted and E200K mutant cell
71 d the shift in translation from polysomes to monosomes is attenuated, suggesting puf3Delta cells perc
72 The sedimentation coefficients of the intact monosome, large subunit, and small subunit were 55, 39,
73   Further, most mRNAs exhibit some degree of monosome occupancy, with monosomes predominating on nons
74 nslational activity of single 80S ribosomes (monosomes) on mRNA is less well understood, even though
75 free 60S ribosomal subunits but not with 80S monosomes or polysomal ribosomes, indicating that it is
76 free 60S ribosomal subunits but not with 80S monosomes or polysomal ribosomes, indicating that it is
77 rge (50S) ribosomal subunit but not with 70S monosomes or with translating ribosomes.
78                                 An increased monosome peak with moderate ribosomal disaggregation in
79 g and activated lymphocytes possess abundant monosome populations, most of which actively translate i
80 ibit some degree of monosome occupancy, with monosomes predominating on nonsense-mediated decay (NMD)
81 ome-translated mRNAs, revealed that head 80S monosomes preferentially translate mRNAs with TOP motifs
82 and was associated with a decreased polysome/monosome ratio that is indicative of reduced protein tra
83  driving pre-rRNA processing defects and 80S monosome reduction, the downstream effects are remarkabl
84  less well understood, even though these 80S monosomes represent the dominant ribosomal complexes in
85 rom heavy polysomes to lighter polysomes and monosomes, suggesting that Gemin5 functions as an activa
86 ofile analysis, where a shift in the mRNA to monosomes was apparent in response to SCD2 silencing.
87 s generate a unique interface not present in monosomes, which can be recognized by Hel2/ZNF598 ubiqui
88                    IF3(mt) does not bind 55S monosomes, while the deletion derivative binds slightly