ライフサイエンスコーパス: monoubiquitination

1 in early FA genes with the absence of FANCD2 monoubiquitination.

2 tes, resulting in an efficient site-specific monoubiquitination.

3 ng activity compromise DNA-stimulated FANCD2 monoubiquitination.

4 lex and biochemical reconstitution of FANCD2 monoubiquitination.

5 measured by the Fanconi D2 protein (FANCD2) monoubiquitination.

6 , another minor pathway contributed to their monoubiquitination.

7 ubiquitin chain elongation and directs Rim8 monoubiquitination.

8 oting nucleosome reassembly coupled with H2B monoubiquitination.

9 , chromosome aberrations, and reduced FANCD2 monoubiquitination.

10 is associated with level of residual FANCD2 monoubiquitination.

11 ed a significant reduction in H2A lysine 119 monoubiquitination.

12 gulated by Pirh2 E3 ubiquitin ligase through monoubiquitination.

13 protein Dam1 by Set1 similarly requires H2B monoubiquitination.

14 ed by DNA damage-induced phosphorylation and monoubiquitination.

15 action with Rad18 but is independent of PCNA monoubiquitination.

16 s well as the activity to down-regulate PCNA monoubiquitination.

17 r other alternative RFCs did not affect PCNA monoubiquitination.

18 tudy functional consequences of FANCI:FANCD2 monoubiquitination.

19 in promoting DNA repair, independent of its monoubiquitination.

20 BMs) of the polymerases and enhanced by PCNA monoubiquitination.

21 ear import of FANCD2, a prerequisite for its monoubiquitination.

22 rity of the FA core complex, and thus FANCD2 monoubiquitination.

23 ls via a mechanism that is dependent on PCNA monoubiquitination.

24 kes place following and independently of H2B monoubiquitination.

25 indin and its E2 enzyme but was defective in monoubiquitination.

26 f FANCL and FANCI in the catalysis of FANCD2 monoubiquitination.

27 eir resistance to MMC re-establishing FANCD2 monoubiquitination.

28 ntering endogenous lesions in the absence of monoubiquitination.

29 ortant role in the regulation of H2A Lys-119 monoubiquitination.

30 ex is recruited before or after the critical monoubiquitination.

31 rt proliferation cell nuclear antigen (PCNA) monoubiquitination.

32 erminus of SMN(K0) and thereby mimicking SMN monoubiquitination.

33 hway, and localizes to ICLs dependent on its monoubiquitination.

34 en proposed to function downstream of FANCD2 monoubiquitination, a critical event in the FA pathway.

35 nds to both Rad18 and PCNA and promotes PCNA monoubiquitination, a function unique to Poleta among Y-

36 8 and FAAP20 are needed for efficient FANCD2 monoubiquitination, a key step of the FA network; RNF8 a

37 and is also inefficient in promoting FANCD2 monoubiquitination, a key step of the Fanconi anemia pat

38 onoubiquitination and the mechanism by which monoubiquitination activates MALT1 had remained elusive.

39 While monoubiquitination activates mutagenic translesion synth

40 Residual FANCD2 monoubiquitination activity is retained in cells defecti

41 ctional interplay is disabled, switching its monoubiquitination activity toward a polyubiquitination

42 ssociated with the extent of residual FANCD2 monoubiquitination activity.

43 Monoubiquitination aids in the nuclear export and entran

44 rotein in the enzymatic activation of FANCD2 monoubiquitination, an essential step in the repair of D

45 crease of Taf1 results in a decrease in Pax3 monoubiquitination, an increase in the levels of Pax3 pr

46 t the ability for RNF168 to promote H2A/H2AX monoubiquitination and 53BP1 IRIF, but not RNF168 self-a

47 more, addition of the FANCI protein enhances monoubiquitination and also restricts it to the in vivo

48 2) targets the key enzyme, CCTalpha, for its monoubiquitination and degradation, thereby reducing syn

49 A critical step in this pathway is the monoubiquitination and deubiquitination of FANCD2.

50 Monoubiquitination and deubiquitination of FANCD2:FANCI

51 Exchange of histone H2A monoubiquitination and deubiquitination reflects the suc

52 Ligand-induced monoubiquitination and endosomal puncta of BIK1 exhibit

53 d MHF2, (2) impairment of DNA damage-induced monoubiquitination and foci formation of FANCD2, (3) def

54 The misregulation of histone H2B monoubiquitination and H3K4 methylation result in the pa

55 PCNA monoubiquitination and TLS in a FANCD2 monoubiquitination and HR-independent manner in response

56 Finally, we find that Deltex2 causes Jmjd1c monoubiquitination and inhibits its demethylase activity

57 otein, is involved in the regulation of PCNA monoubiquitination and interacts directly with Rad18 on

58 es S-phase and DNA damage-inducible FANCD2/I monoubiquitination and nuclear foci formation.

59 n remains competent for DNA damage-inducible monoubiquitination and phosphorylation.

60 nterstrand cross-links independently of PCNA monoubiquitination and Poleta, whereas RAD18 plus Poleta

61 ass spectrometry analysis revealed that both monoubiquitination and polyubiquitination (via both K48-

62 TLS and TS depend on site-specific PCNA K164 monoubiquitination and polyubiquitination, respectively.

63 that levels of Pax3 protein are regulated by monoubiquitination and proteasomal degradation during po

64 -catalytic role for Poleta in promoting PCNA monoubiquitination and provide a new potential mechanism

65 athway, an upstream FA core complex mediates monoubiquitination and recruitment of the central FANCD2

66 Following UV, HLTF enhances PCNA monoubiquitination and recruitment of TLS polymerase eta

67 the ~300 kilodalton ID complex reveals that monoubiquitination and regulatory phosphorylation sites

68 gs highlight an E3-independent mechanism for monoubiquitination and reveal mechanistic details of SET

69 The UBZ4 motif is required for PCNA monoubiquitination and survival after UV damage.

70 Here, we show that H-Ras is activated by monoubiquitination and that ubiquitination at Lys-117 ac

71 in the FA pathway at both signaling through monoubiquitination and the ensuing DNA repair.

72 We found that depletion of MSH2 impairs PCNA monoubiquitination and the formation of foci containing

73 The molecular requirements for MALT1 monoubiquitination and the mechanism by which monoubiqui

74 the FA nuclear core complex, regulate FANCD2 monoubiquitination and the telomeric localization of FAN

75 CD2 and RAD51 have an important role in PCNA monoubiquitination and TLS in a FANCD2 monoubiquitinatio

76 or proliferating cell nuclear antigen (PCNA) monoubiquitination and TLS polymerase recruitment; howev

77 APC/C(Cdh1) in modulating the status of PCNA monoubiquitination and UV DNA repair before S phase entr

78 e of a proper DNA ligand in FANCD2 and FANCI monoubiquitination, and reveal regulatory mechanisms tha

79 inducing proliferating cell nuclear antigen monoubiquitination, and suppressing mutagenesis.

80 ) cells and that gammaH2AX, PCNA, and FANCD2 monoubiquitinations are induced by oxaliplatin in parent

81 nd RAD18, the E3 ligase responsible for PCNA monoubiquitination, are specifically required for ATM si

82 ese experiments identify RAD18-mediated PCNA monoubiquitination as a central hub for the mobilization

83 Here, we analyzed histone H2B monoubiquitination as one such chromatin modification by

84 showed that USP7 is also a key regulator for monoubiquitination at H2A Lys-119 as both knockdown and

85 recruitment of 53BP1 to DNA damage and H2AX monoubiquitination at K13/15.

86 ism of Ras activation is distinct from K-Ras monoubiquitination at Lys-147, which leads to impaired r

87 and/or WW and Hect domains that favors WWP1 monoubiquitination at the expense of its polyubiquitinat

88 related with increased levels of histone H2B monoubiquitination, at the reelin promoter.

89 Human Bre1, an E3 ligase for H2B monoubiquitination, binds p53 and enhances activator-dep

90 is dispensable for ICL unhooking and FANCD2 monoubiquitination but is essential for HR, confirming t

91 absence of FANCD2, DNA also stimulates FANCI monoubiquitination, but in a FANCL-independent manner.

92 ed for the maintenance of high levels of H2B monoubiquitination, but not for H3K4 and H3K79 trimethyl

93 FL118 inhibits p53 polyubiquitination and monoubiquitination by Mdm2-MdmX E3 complex in cells and

94 First, we determined that PCNA monoubiquitination by RAD18 is necessary for efficient b

95 Second, we showed that in addition to PCNA monoubiquitination by RAD18, the Fanconi anemia core com

96 Histone H2B monoubiquitination by Rad6/Bre1 is required for the trim

97 Whereas monoubiquitination by Rad6/Rad18 mediates TLS, extension

98 esting that USP7 facilitates UV-induced PCNA monoubiquitination by stabilizing Poleta.

99 Among these is monoubiquitination, catalyzed by the NEDD4 family ubiqui

100 Although monoubiquitination commonly confers nondegradative activ

101 The ID2 complex is a poor substrate for monoubiquitination, consistent with the published crysta

102 biquitination, and shows that ligand-induced monoubiquitination contributes to the release of BIK1 fa

103 reas HSV-1 productive growth was impaired in monoubiquitination-defective FA cells, this restriction

104 by specialized DNA polymerases in both PCNA monoubiquitination-dependent and -independent fashions.

105 In this review, the process of histone H2B monoubiquitination-dependent and -independent histone H3

106 ferases; however, dSet1/COMPASS is the major monoubiquitination-dependent H3K4 di- and trimethylase i

107 ogether, our findings demonstrate a distinct monoubiquitination-dependent mechanism that is required

108 Monoubiquitination did not cause Gsk3beta degradation no

109 ues with functional studies we show that H2B monoubiquitination does not influence global gene expres

110 We report that monoubiquitination does not promote any specific exogeno

111 t be degraded via APC/C(Cdh1) inhibited PCNA monoubiquitination during G1, likely compromising the re

112 cellular activator that converts Mdm2 from a monoubiquitination E3 ligase to a polyubiquitination E3

113 TRIM37-directed histone H2A monoubiquitination enforces changes in DNA repair that r

114 a ubiquitin-APE1 fusion gene suggested that monoubiquitination enhanced the gene suppression activit

115 ork serves as the trigger for the activating monoubiquitination event.

116 Simple monoubiquitination events coexist with more complex form

117 to di- and trimethylation requires prior H2B monoubiquitination followed by recruitment of the Cps35

118 hocyte priming through removal of inhibitory monoubiquitination from ZAP70.

119 unravel a unique molecular mechanism whereby monoubiquitination governs the trafficking and life span

120 lucose starvation suppresses histone 2A K119 monoubiquitination (H2Aub), a histone modification that

121 methylation of histone H3 Lys4 (H3K4) by H2B monoubiquitination (H2Bub) has been widely studied, with

122 Although H2B monoubiquitination (H2Bub) is well known as a prerequisi

123 own targets of the HUB1-mediated histone H2B monoubiquitination (H2Bub).

124 Histone H2B monoubiquitination (H2Bub1) is catalyzed by the RNF20 co

125 Histone H2B monoubiquitination (H2Bub1) is centrally involved in gen

126 The cellular regulation of FANCD2/I monoubiquitination, however, remains poorly understood.

127 ow that the H2AX K120R mutant abolishes H2AX monoubiquitination, impairs the recruitment of p-ATM (Se

128 Here we report that Stx3 undergoes monoubiquitination in a conserved polybasic domain.

129 nse variants with drastically reduced FANCD2 monoubiquitination in biochemical and/or cell-based assa

130 These data reveal a new role for monoubiquitination in controlling Rad18 function and sug

131 Our findings also reveal how monoubiquitination in general can induce an alternative

132 ed proliferating cell nuclear antigen (PCNA) monoubiquitination in Poleta-proficient but not in Polet

133 ni anemia (FA) core complex, promotes FANCD2 monoubiquitination in response to DNA damage, and suppre

134 51, but not BRCA2, is also required for PCNA monoubiquitination in response to HU, suggesting that th

135 ng proliferating cell nuclear antigen (PCNA) monoubiquitination in response to ultraviolet (UV) damag

136 hase and interacted with PCNA to promote its monoubiquitination in response to UV-induced damage for

137 capsid protein) was found to be targeted for monoubiquitination in transfected mammalian cells.

138 promote efficient DNA damage-induced FANCD2 monoubiquitination in vertebrate cells, suggesting an im

139 CL and Ube2T, and is not required for FANCD2 monoubiquitination in vitro.

140 ound to target histone H2B at lysine 120 for monoubiquitination in vitro.

141 This monoubiquitination, in turn, contributes to the activity

142 formation without detectable changes in PCNA monoubiquitination, indicating that MSH2 can regulate po

143 FANCI:FANCD2 monoubiquitination is a critical event for replication f

144 In sum, histone H2B monoubiquitination is an important chromatin modificatio

145 x-independent manner, suggesting that FANCD2 monoubiquitination is dispensable for its interaction wi

146 The resulting constitutive lysine-867 monoubiquitination is essential for SETDB1's enzymatic a

147 Here we show that Pax3 monoubiquitination is mediated by the ubiquitin-activati

148 However, the loss of H2B monoubiquitination is not suppressed by this mutation, w

149 required for this enhanced interaction, its monoubiquitination is not.

150 patients, inactivation of PolH by Pirh2 via monoubiquitination is one of the mechanisms by which Pol

151 tion of Bre1, the E3 ligase required for H2B monoubiquitination, leads specifically to reduced bulk H

152 Our study demonstrates that the monoubiquitination machinery and Cps35/Swd2 function to

153 ubiquitin and the NLS, we show that CCTalpha monoubiquitination masks its NLS, resulting in cytoplasm

154 t, indicate that deregulation of histone H2B monoubiquitination may contribute to cancer development.

155 ve normal levels of H3K4me3, suggesting that monoubiquitination may not directly stimulate COMPASS bu

156 B, FANCL, and FAAP100) that functions as the monoubiquitination module.

157 ycin C and ionizing radiation induced FANCD2 monoubiquitination, neither could induce the association

158 Therefore, monoubiquitination not only is a prerequisite for degrad

159 e FA core ubiquitin ligase complex-dependent monoubiquitination of 2 interacting FA proteins, FANCI a

160 key molecular step in the FA pathway is the monoubiquitination of a pseudosymmetric heterodimer of F

161 We show MID1-dependent monoubiquitination of alpha4 triggers calpain-mediated c

162 says, individual MID1 E3 domains facilitated monoubiquitination of alpha4, whereas full-length MID1 a

163 raction occurs in the cytoplasm and requires monoubiquitination of an evolutionarily conserved lysine

164 -H2 FINGER A3A (RHA3A) and RHA3B mediate the monoubiquitination of BIK1, which is essential for the s

165 Monoubiquitination of CHIP by Ube2w stabilizes the inter

166 of ribosomes collided at aberrant mRNAs and monoubiquitination of distinct small ribosomal subunit p

167 Expression of CDK5 enhanced monoubiquitination of endogenous APE1.

168 Ribosome profiling showed that the monoubiquitination of eS7A was crucial for translational

169 Not4-mediated monoubiquitination of eS7A was required for resistance t

170 tivation in HSV-1-infected cells resulted in monoubiquitination of FA effector proteins FANCI and FAN

171 a and Rad3-related (ATR) kinase, followed by monoubiquitination of FANCD2 and FANCI by the FA core co

172 the FA core complex that is responsible for monoubiquitination of FANCD2 and FANCI.

173 stalled replication forks by catalyzing the monoubiquitination of FANCD2 and FANCI.

174 complex functions as a signaling machine for monoubiquitination of FANCD2 and FANCI.

175 eins (A/B/C/E/F/G/L/M), is essential for the monoubiquitination of FANCD2 and FANCI.

176 le the FA E3 ligase complex, which catalyzes monoubiquitination of FANCD2 and is essential for replic

177 step in the activation of FA pathway is the monoubiquitination of FANCD2 and its binding partner FAN

178 A critical step in the pathway is the monoubiquitination of FANCD2 by the RING E3 ligase FANCL

179 However, depletion of Tip60 did not reduce monoubiquitination of FANCD2 or its localization to nucl

180 However, how monoubiquitination of FANCD2 promotes ICL repair at the

181 s were hypersensitive to MMC and MMC-induced monoubiquitination of FANCD2 was impaired.

182 The FA core complex catalyzes the monoubiquitination of FANCD2, and this event is essentia

183 Central to this pathway is the monoubiquitination of FANCD2, which coordinates multiple

184 critical event in activating this pathway is monoubiquitination of FANCD2.

185 in-binding domain (UBZ), the ID complex, and monoubiquitination of FANCD2.

186 ubiquitination, which significantly precedes monoubiquitination of FANCI; moreover, monoubiquitinatio

187 Monoubiquitination of Fanconi anemia group D2 protein (F

188 on to evaluate S phase checkpoint integrity, monoubiquitination of Fanconi protein D2, ATM protein ex

189 We found that "DNA-free" RPA inhibits monoubiquitination of free PCNA by directly interacting

190 found that Wnt stimulation induced prolonged monoubiquitination of Gsk3beta and Gsk3beta-beta-TrCP in

191 Rather, increased monoubiquitination of Gsk3beta/Gsk3beta-beta-TrCP associ

192 2A-HUB catalyzes monoubiquitination of H2A at lysine 119, functioning as

193 demonstrate that UbE2E1 is critical for the monoubiquitination of H2A at residue Lys-119 (uH2AK119)

194 idic patch on histone H4 to achieve specific monoubiquitination of H2A.

195 A damage response (DDR) factor that promotes monoubiquitination of H2A/H2AX at K13/15, facilitates re

196 damage-dependent manner and is required for monoubiquitination of H2AX at Lys(119)/Lys(120).

197 Mechanistically, we show that monoubiquitination of H2AX induced by RING finger protei

198 Our study therefore suggests that monoubiquitination of H2AX is an important step for DNA

199 Here, we show that monoubiquitination of H2AX is induced upon DNA double st

200 Here we show that monoubiquitination of H2AX mediated by the RNF2-BMI1 com

201 These data suggest that monoubiquitination of H2AX plays a critical role in init

202 Importantly, a defect in monoubiquitination of H2AX profoundly enhances ionizing

203 f actively transcribed genes that depends on monoubiquitination of H2B K120 (H2B-Ub) and is an exampl

204 ency of methylation at H3K4 and H3K79 on the monoubiquitination of H2BK123 was recently challenged, a

205 Monoubiquitination of histone H2A (H2AUb1) is a reversib

206 1 (PRC1) mediates gene silencing, in part by monoubiquitination of histone H2A on lysine 119 (uH2A).

207 This molecular complex increased the monoubiquitination of histone H2A(Lys119), a characteris

208 of covalent histone modifications, including monoubiquitination of histone H2A, and the molecular mec

209 Monoubiquitination of histone H2B (H2B-Ub) plays a role

210 on of histone H3K4 and H3K79 is dependent on monoubiquitination of histone H2B (H2B-Ub).

211 We previously showed that monoubiquitination of histone H2B (ubH2B) is highly dyna

212 We show that Smurf2 regulates the monoubiquitination of histone H2B as well as the trimeth

213 harbors E3 ubiquitin ligase activity toward monoubiquitination of histone H2B at lysine120 (H2BK120U

214 trimethylation of H3K79 by Dot1 requires the monoubiquitination of histone H2B by the Rad6/Bre1 compl

215 litation of GGR is achieved through enabling monoubiquitination of histone H2B lysine 123 by Bre1, wh

216 Monoubiquitination of histone H2B on Lys 123 (H2BK123ub)

217 Monoubiquitination of histone H2B plays a central role i

218 r yeast homolog Bre1 as ubiquitin ligases in monoubiquitination of histone H2B.

219 he Set1 methyltransferase and requires prior monoubiquitination of histone H2B.

220 (E2) UBE2T and ubiquitin ligase (E3) FANCL, monoubiquitination of human FANCD2 and FANCI was examine

221 Monoubiquitination of ID is essential for ICL repair by

222 We conclude that Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and

223 f either FANCI or FANCD2 is known to prevent monoubiquitination of its respective partner, it is uncl

224 This clamping requires monoubiquitination of only the FANCD2 subunit.

225 09 back to the Otub1(K0) mutant restores the monoubiquitination of Otub1 and its function to stabiliz

226 T tag and non-GST-tagged Mdm2 only catalyzes monoubiquitination of p53 even at extremely high concent

227 a-dependent lesion bypass or RAD18-dependent monoubiquitination of PCNA are not necessary to promote

228 In this review, we focus on the monoubiquitination of PCNA by Rad6/Rad18 and summarize t

229 ellular DNA replication processes as well as monoubiquitination of PCNA in response to UV damage.

230 Moreover, RAD18-dependent monoubiquitination of PCNA is required for Mitomycin C a

231 and this activity was required for efficient monoubiquitination of PCNA on DNA.

232 and this interaction may selectively promote monoubiquitination of PCNA on long RPA-coated ssDNA.

233 quitin ligase, which activates TLS repair by monoubiquitination of PCNA, is also affected by BPLF1 de

234 biquitin-conjugation enzyme, is required for monoubiquitination of Pex20.

235 Importantly, while monoubiquitination of poleta precludes its ability to in

236 Moreover, we show that monoubiquitination of PolH alters the ability of PolH to

237 We also show that monoubiquitination of PolH inhibits the ability of PolH

238 Monoubiquitination of proliferating cell nuclear antigen

239 both the stalling of the holoenzyme and the monoubiquitination of proliferating cell nuclear antigen

240 Human TLS requires selective monoubiquitination of proliferating cell nuclear antigen

241 These findings suggest that the monoubiquitination of ribosomal protein eS7A plays a cru

242 Monoubiquitination of SMN has a mild effect on promoting

243 discover that Itch monoubiquitinates SMN and monoubiquitination of SMN plays an important role in reg

244 Here, we reveal the mechanism by which monoubiquitination of specific H2A lysine residues alter

245 Therefore, monoubiquitination of Syn5 in early mitosis disrupts SNA

246 (FA proteins A/B/C/E/F/G/L/M) that mediates monoubiquitination of the downstream targets FANCD2 and

247 e, we have found that L. pneumophila induces monoubiquitination of the E2 enzyme UBE2N by its Dot/Icm

248 vation of the FA-BRCA pathway occurs via the monoubiquitination of the FANCD2 and FANCI proteins, tar

249 p in the activation of the FA pathway is the monoubiquitination of the FANCD2 and FANCI proteins, whi

250 A critical step is the monoubiquitination of the FANCD2 protein, and cells from

251 umor suppressor pathway is the site-specific monoubiquitination of the FANCD2 protein.

252 522, is a critical residue for mediating the monoubiquitination of the FANCD2-FANCI complex.

253 A key step in crosslink repair is monoubiquitination of the FANCD2-FANCI heterodimer, whic

254 At the heart of this pathway is the monoubiquitination of the FANCI-FANCD2 (ID) complex by t

255 as ubiquitin ligase activity responsible for monoubiquitination of the FANCI-FANCD2 (ID) complex, whi

256 n of the UBE2T ubiquitin conjugase is in the monoubiquitination of the FANCI-FANCD2 heterodimer, a ce

257 n studies, we found that C/EBPdelta promotes monoubiquitination of the Fanconi anemia complementation

258 tion fork stalls at an ICL(2); this triggers monoubiquitination of the ID complex, in which one ubiqu

259 ollectively, these findings suggest that the monoubiquitination of the PIV5 M protein is important fo

260 cells and in vitro, through Ube2W-catalyzed monoubiquitination of TRIM5alpha.

261 In addition, polyubiquitination (but not monoubiquitination) of MuRF1 allowed S5a to bind to MuRF

262 e show that Ufd2p catalyses K48-linked multi-monoubiquitination on K29-linked ubiquitin chains assemb

263 inase (PR-DUB) complexes catalyze removal of monoubiquitination on lysine 119 of histone H2A (H2AK119

264 MALT1 activation requires its monoubiquitination on lysine 644 (K644) within the Ig3 d

265 tabilizes BCLb protein, while also promoting monoubiquitination on multiple lysine residues and reloc

266 tiated by proliferating cell nuclear antigen monoubiquitination or less well-characterized fork rever

267 Proliferating cell nuclear antigen monoubiquitination positively regulates TLS to overcome

268 nesis, but none of the key regulators of the monoubiquitination process were known.

269 ghts into the Otub1 inhibition of E2 wherein monoubiquitination promotes the interaction of Otub1 wit

270 Depletion of C1orf124 compromises PCNA monoubiquitination, RAD18 chromatin association, and RAD

271 H3K79 methylation is solely dependent on H2B monoubiquitination regardless of any additional alterati

272 Poliota monoubiquitination remains unchanged after cells are exp

273 cedes monoubiquitination of FANCI; moreover, monoubiquitination responses of FANCD2 and FANCI exhibit

274 While the majority of RPS2 and RPS3 monoubiquitination resulted from ZNF598-dependent sensin

275 rotein levels through its ability to mediate monoubiquitination, revealing a critical interaction bet

276 Mutation of the monoubiquitination site in Jmjd1c abolishes the inhibito

277 Mutation of the monoubiquitination site of FANCD2 (K561R) preserves inte

278 atin colocalization but did not inhibit PCNA monoubiquitination, suggesting that T2AA hinders interac

279 ating cell nuclear antigen (PCNA) and FANCD2 monoubiquitinations (surrogate markers of TLS and FA pat

280 temporal and spatial control of FANCD2:FANCI monoubiquitination that is critical for chemotherapy res

281 zation of FANCI is crucial for robust FANCD2 monoubiquitination, the putative FANCI EDGE motif is imp

282 Cbl-b binds and promotes monoubiquitination to CARMA1, a critical signaling molec

283 ect of the loss of either nuclease on FANCD2 monoubiquitination to determine if the nucleolytic proce

284 on, and biochemical reconstitution of FANCD2 monoubiquitination to determine the pathogenicity of eac

285 tant signaling pathway linking Cbl-b-induced monoubiquitination to NFkappaB activation in NKT cell an

286 tion stress depends on PCNA, which undergoes monoubiquitination to stimulate direct bypass of DNA les

287 ANCE act as substrate receptors and restrict monoubiquitination to the FANCD2:FANCI heterodimer in on

288 L DNA-which we also report here-we show that monoubiquitination triggers a complete rearrangement of

289 one H3 lysine 27 (H3K27) methylation and H2A monoubiquitination (ubH2A) are two closely related histo

290 n vitro assay to quantitatively monitor PCNA monoubiquitination under in vivo scenarios.

291 posttranslational modifications (inhibitory monoubiquitination versus activatory phosphorylation), a

292 PCNA monoubiquitination via CRL4(Cdt2) is constitutively anta

293 To date, all H2B monoubiquitination was attributed to the human homolog o

294 ive MALT1 mutants that overcome the need for monoubiquitination, we reveal an allosteric communicatio

295 Conversely, variants with near-normal FANCD2 monoubiquitination were associated with more favorable o

296 e in H3K79 methylation and a decrease in H2B monoubiquitination, which promotes transcription.

297 Dissociation coincides with FANCD2 monoubiquitination, which significantly precedes monoubi

298 Thus, WRNIP1 connects PCNA monoubiquitination with ATMIN/ATM to activate ATM signal

299 Importantly, FANCD2 monoubiquitination within the ID2 complex is strongly st

300 resulted in an increase in the level of PCNA monoubiquitination without affecting the level of FANCD2