ライフサイエンスコーパス: morpholino

1 ed hepatic H2HR in Mdr2(-/-) mice using vivo-morpholino.

2 afish through injection of a splice-blocking morpholino.

3 ockdown approach with two different splicing morpholinos.

4 HMT expression was knocked down by antisense morpholinos.

5 ovel zebrafish model of CNM2 using antisense morpholinos.

6 sgenic insertions, genes with antibodies and morpholinos.

7 and in X. laevis embryos treated with Gas2l2 morpholinos.

8 nse appears to be specific to the tbxt/tbxt2 morpholinos.

9 slation blocking and splice-site interfering morpholinos.

10 (difluoromethyl)-5-(4-(3-methylmorpholino)-6-morpholino-1,3,5-triazin-2-yl)pyridin-2-amine (PQR530, c

11 ed chelator, 2,6-bis[hydroxy(methyl)amino]-4-morpholino-1,3,5-triazine (H(2)BHT).

12 Mice fed D- threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), an inhibitor of glucosyl

13 ynthesis, D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), solubilized in vehicle (

14 ibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol and phospholipase C), we demonstra

15 1-(3,4-dichlorophenyl)-3-(4-methoxyphenyl)-4-morpholino-1H-pyrrole-2,5-dione, named RI-2 (referred to

16 ore (e.g., 2-(4-ethylpiperazin-1-yl)-N-(4-(2-morpholino-4-oxo-4H-chromen-8-yl)dibenzo[b,d]th io-phen-

17 ization of a series of 8-(1-anilino)ethyl)-2-morpholino-4-oxo-4H-chromene-6-carboxamides as PI3Kbeta/

18 Analogues of (dibenzo[b,d]thiophen-4-yl)-2-morpholino-4H-chromen-4-one (NU7441), a potent inhibitor

19 This work resulted in the discovery of the 5-morpholino-7H-thieno[3,2-b]pyran-7-one system as the fou

20 Morpholinos against N-terminal zebrafish Ahi1, orthologo

21 Moreover, zebrafish treated with morpholinos against tubgcp4 were found to have reduced h

22 suppression of IL10 using an antisense IL10 morpholino also extended the tumor growth delay induced

23 Importantly, NEDD9 inhibition by Vivo-Morpholinos, an antisense therapy, decreases primary tum

24 function with dominant-negative Rab4 or Rab4 morpholino and constitutive activation of Rab5 decreases

25 Using morpholino and mutant zebrafish models, we show that POU

26 stage and antiangiogenic drugs such as Vegf morpholino and sunitinib could potentially interfere wit

27 -injecting sub-phenotypic dosages of the two morpholinos and could be rescued by human USB1 RNA.

28 vation of astrocytic Gq signaling or in vivo morpholinos and determined the ability to adapt to novel

29 Importantly, knockdown of ZF gamma1 with morpholinos and disruption of oligomers with the molecul

30 erexpression enhances differentiation, while morpholino- and siRNA-induced knockdown diminishes it.

31 cs, phenotypes, genotypes, gene expressions, morpholinos, antibodies, anatomical structures and publi

32 nt of the conceptus, we conducted an in vivo morpholino antisense oligonucleotide (MAO)-mediated knoc

33 Duchenne muscular dystrophy (DMD), employing morpholino antisense oligonucleotides (PMO-AO) to exclud

34 a common side effect of translation-blocking morpholino antisense oligonucleotides is the induction o

35 and the effects of reducing expression, with morpholino antisense oligonucleotides, on biliary develo

36 slation of the RNA-binding protein Nanos2 by morpholino antisense oligonucleotides, or knockout of th

37 Moreover, inhibition of miR-183 with morpholino antisense oligos in cochlear organotypic cult

38 We used a morpholino antisense strategy to knock down the beta1 or

39 logical plasticity on cued heroin seeking, a morpholino antisense strategy was used to knock down exp

40 esultant ocular coloboma phenotype following morpholino antisense translation-blocking knockdown and

41 Morpholino-antisense-mediated depletion of fibulin-7B, a

42 Using a morpholino-antisense-oligonucleotide-based zebrafish mod

43 Suppression of CD47 by morpholino approach suppressed growth of HCC in vivo and

44 b by specific small interfering RNA and Vivo-Morpholino, as well as inhibition of Smyd2 by its specif

45 sin conjugation also improved the potency of morpholino ASO designed to correct splicing of survival

46 Co-injection of tuba and cdc42 morpholinos at low doses, which alone had no effect, res

47 ), gene knockdown of either CBS or CSE using morpholinos attenuated the hypoxic ventilatory response.

48 , locked nucleic acid, or phosphorodiamidate morpholino backbones.

49 (E1), located upstream of SMN2 exon 7 using Morpholino-based antisense oligonucleotides (E1(MO)-ASOs

50 Morpholino-based experimentation confirmed that modulati

51 idate genes for angiogenesis, we performed a morpholino-based genetic screen in zebrafish and identif

52 Morpholino-based knockdown of a zebrafish PYCR2 ortholog

53 based on two-photon microscopy revealed that morpholino-based knockdown of glutaredoxin 2 in zebrafis

54 Morpholino-based knockdown of the Xenopus ortholog of CC

55 onger than defects associated with transient morpholino-based knockdown.

56 Morpholino-based loss of cdk10 expression caused apoptos

57 infusion of farnesoid X receptor (FXR) Vivo-morpholino before AOM injection.

58 activity relationship studies on 7-methoxy-4-morpholino-benzothiazole derivatives featured by aryloxy

59 We found that indels within the Morpholino binding site are indeed able to suppress both

60 Nonetheless, mutating the morpholino binding sites in both maternal and zygotic ge

61 mice were treated with mismatch or H2HR vivo-morpholino by tail vein injection for 1 week.

62 Thus, this morpholino can be used for concurrent suppression of hem

63 d tools such as siRNA, antisense oligos, and morpholinos can be used to silence expression of specifi

64 sor is made of a monolayer of charge-neutral morpholino capture probes on an indium tin oxide (ITO)-c

65 Knockdown of Dvr1 by morpholino causes dramatically reduced or absent express

66 Morpholino co-injection experiments identify ccm2l as an

67 ROS in NOX4 embryos were attenuated by NOX4 morpholino co-injection, treatments of the embryos with

68 normal and BDL rats treated with AANAT Vivo-Morpholino, compared to controls.

69 ment for cardiac phenotypes as compared with morpholino controls.

70 Live-cell imaging and morpholino depletion of axonemal Paralyzed Flagella 16 i

71 , whereas the remaining PMO analogues having morpholino, dimethylamino, or N-methylamino phosphorodia

72 Conversely, a Vivo-Morpholino directed at mouse Gls had no antitumor activi

73 Zebrafish injected with morpholinos directed against pkd1 manifest severe bone c

74 ese effects are abolished by co-injection of morpholinos directed against TAZ.

75 stages, and that this increase is blocked by morpholino-directed depletion of 12-LOX.

76 knockdown of Lmx1b and FoxC orthologs using morpholino doses that caused no or minimal phenotypic ch

77 Treating optic cups with an antisense morpholino effectively blocked aberrant splicing and res

78 In one crystal form, a molecule of Mes [2-(N-morpholino)ethane sulfonic acid] mimics the target uridi

79 Embryos injected with an nkx2.5 morpholino exhibited SHF phenotypes caused by compromise

80 Using a morpholino for in vivo knock-down of G6f-like levels in

81 ve study presented here indicates that using morpholinos for targeted gene knockdowns remains of cons

82 This new series also features a morpholino glycone previously reported in semisynthetic

83 However, the anti conformation of 8-morpholino-GMP is selected by Bs-FtsZ, while Mj-FtsZ bin

84 Pharmacological antagonists and morpholinos implicate p38 and Jun kinases and MEF2C in t

85 Treatment with H2HR vivo-morpholino in Mdr2(-/-)-mice decreased hepatic damage; H

86 biting RIP3 protein induction with antisense morpholinos in wild-type animals or using RIP3-deficient

87 that suppression of CD47 using an antisense morpholino increases survival of mice exposed to lethal

88 When discrepancy between mutant and morpholino-induced (morphant) phenotypes is observed, ex

89 d that mutant human NNT failed to rescue nnt morpholino-induced heart dysfunction, indicating a proba

90 Morpholino-induced knockdown of KCNE4 depolarized mesent

91 Morpholino-induced knockdown of sgol1 in zebrafish recap

92 Morpholino-induced loss of heparanase 2 caused embryonic

93 re we describe the phenotype associated with morpholino-induced otoferlin knockdown in zebrafish and

94 mRNA transcripts were able to rescue abcc6a morpholino-induced phenotype of zebrafish.

95 Minimal rescue of the morpholino-induced phenotype was achieved with eight of

96 sted in zebrafish, and minimal rescue of the morpholino-induced phenotype was found.

97 erent genes failed to recapitulate published Morpholino-induced phenotypes (morphants).

98 In a zebrafish model, overexpression or morpholino-induced suppression of foxc1 induced cerebral

99 Morpholino-induced transient gene knockdown was performe

100 use cornea, subconjunctival injection of the morpholino-inhibited corneal angiogenesis and lymphangio

101 Reducing expression of TRPC1 by antisense morpholinos inhibits the effects of MS channel blockers

102 Electron microscopy analysis revealed that morpholino-injected embryos lacked a lamina densa and la

103 bution of rods in lor/tbx2b(p25bbtl) or six7 morpholino-injected larvae protect from pde6c(w59)-induc

104 Disruption of ccm2l by morpholino injection causes dilation of the atrium and i

105 Depletion of CenpH by morpholino injection decreased cyclin B1 levels, resulti

106 Intravitreal morpholino injection suppressed laser choroidal neovascu

107 when CAMSAP3 expression was knocked down by morpholino injection.

108 e by first generating boranephosphoroamidate morpholino internucleotide linkages followed by oxidativ

109 wn of complexin by injection of an antisense morpholino into zebrafish embryos prevented photorecepto

110 in branchial arch ectoderm and endoderm, and morpholino knock-down of foxi1 also causes apoptosis of

111 Morpholino knock-down of zebrafish alx1 expression cause

112 We use morpholino knockdown and a Clstn-1 mutant to show that C

113 onfidence cardiac predictions using targeted morpholino knockdown and initial blinded phenotyping in

114 in Xenopus tropicalis, where ZIP12 antisense morpholino knockdown impairs neural tube closure and arr

115 and rbm24a-CRISPR/Cas9-targeted mutation or morpholino knockdown in zebrafish, results in Sox2 downr

116 y homologs and can engulf V. coralliilyticus Morpholino knockdown indicates that Nv-TLR also has an e

117 Using both genetic mutant and morpholino knockdown models, we found that loss of Fer1l

118 F60a or BAF60b expression and are rescued by morpholino knockdown of BAF60a or BAF60b.

119 Morpholino knockdown of COLXIV-A provoked a skin detachm

120 Morpholino knockdown of cspp1 in zebrafish caused phenot

121 n the nascent mesoderm and neurectoderm, and morpholino knockdown of either causes defects in differe

122 Consistent with its expression, morpholino knockdown of Gtpbp2 causes defects in ventral

123 he BMP branch of the TGFbeta superfamily, as morpholino knockdown of Gtpbp2 decreases, and overexpres

124 Morpholino knockdown of matrilin-1 results both in overa

125 Chemical inhibition and morpholino knockdown of nuclear estrogen receptor 2b (es

126 By using zebrafish morpholino knockdown of PCD candidate genes as an in viv

127 owth and elongation appear normal, antisense morpholino knockdown of pcdh18b results in dose-dependen

128 Morpholino knockdown of peroxidasin in zebrafish reveale

129 Using morpholino knockdown of PmTPCs and PmARC in the oocytes,

130 Morpholino knockdown of RNF207 in zebrafish embryos resu

131 encodes a transcription factor and for which morpholino knockdown of the ortholog in zebrafish result

132 Morpholino knockdown of the zebrafish homologue dachsous

133 Morpholino knockdown significantly reduced the expressio

134 tients with the deletions, we used zebrafish morpholino knockdown to test the function of each orthol

135 Using a PADI1-specific inhibitor and Padi1-morpholino knockdown, we found that citrullination of hi

136 Using morpholino knockdown, we show that NMIIA and NMIIB are b

137 llar length defects that mirror defects with morpholino knockdown.

138 established a zebrafish model of PN using a morpholino-knockdown approach with two different splicin

139 are replicated in X. tropicalis larvae with morpholino knockdowns, in which expression of truncated

140 n Xenopus embryos and that splicing-blocking morpholinos lead to unexpected off-target mis-splicing e

141 Using morpholino-mediated ablation of Slc38a8 in medaka fish,

142 In an in vivo model, we show that morpholino-mediated dpy30 knockdown resulted in severe d

143 ith none of the side effects associated with morpholino-mediated Duox1 knockdown.

144 CNG-modulin in phototransduction in vivo in morpholino-mediated gene knockdown zebrafish.

145 Morpholino-mediated inhibition of Qk translation confirm

146 We show that morpholino-mediated inpp5k loss of function in the zebra

147 Its morpholino-mediated knockdown affects neural crest precu

148 es, we show that pharmacological inhibition, morpholino-mediated knockdown and CRISPR/cas9-mediated k

149 Morpholino-mediated knockdown and transient overexpressi

150 Importantly, a morpholino-mediated knockdown in Xenopus revealed that p

151 This study employed expression analysis and morpholino-mediated knockdown in zebrafish in concert wi

152 Morpholino-mediated knockdown of each expressed Wnt liga

153 in vivo multiphoton microscopy, we show that morpholino-mediated knockdown of enox1 increases NADH le

154 We demonstrate that morpholino-mediated knockdown of GPR31, a purported G-pr

155 Morpholino-mediated knockdown of NvecGrl1 causes develop

156 Morpholino-mediated knockdown of s1pr1 causes global and

157 Morpholino-mediated knockdown of the two MYO9A orthologu

158 Morpholino-mediated knockdown of TNFalpha expression bef

159 ecular screens and tests of gene function by morpholino-mediated knockdown, we identified SoxC and Br

160 Using morpholino-mediated knockdown, we specifically targeted

161 pment but thus far has only been studied via morpholino-mediated knockdown.

162 To study protein function, we used morpholino-mediated knockdowns in zebrafish and short ha

163 Morpholino-mediated loss-of-function experiments show th

164 with the phenotypes of RP2 knockout mice and morpholino-mediated RP2 knockdown zebrafish.

165 Morpholino-mediated Sesn3 knockdown in zebrafish confirm

166 Vivo morpholino-mediated silencing of SOCS1 and SOCS3 resulte

167 Morpholino-mediated suppression of Enox1 in Tg(fli1-eGFP

168 Using morpholino-mediated translation inhibition, we demonstra

169 Vivo-morpholino-mediated WT1 knockdown decreased Kdr transcri

170 antitative RT-PCR, in situ hybridization and morpholinos-mediated knockdown.

171 3-amino-2-oxazolidinone (AOZ) and 3-amino-5-morpholino-methyl-2-oxazolidinone (AMOZ) side-chains of

172 ed and then reversibly surface bonded onto a morpholino microarray for hybridization.

173 ntration device incorporating charge-neutral morpholino (MO) probes: as DNA analyte is concentrated i

174 We performed antisense morpholino (MO) studies in Danio rerio to characterize t

175 Injection of morpholino (MO)-modified antisense oligonucleotides spec

176 specific antagonists and, respectively, with morpholinos (MO) against S1PR2 and S1PR5a-which represen

177 SO10-29 (MOE10-29) was more efficacious than morpholino-modified ASO10-29 (PMO10-29) at the same mola

178 When compared with morpholino, molecular beacon is 2 orders of magnitude mo

179 bryos using splice- and translation-blocking morpholinos (MOs) caused pronephric cysts, hydrocephalus

180 rface hybridization reaction between DNA and morpholinos (MOs) for enhanced detection.

181 Morpholinos (MOs) sometimes produce off-target or toxici

182 lated mRNAs or gene-specific oligonucleotide morpholinos (MOs), respectively.

183 Morpholino-nitroalkenes were assumed to convert into the

184 the multicomponent reaction of aminoazoles, morpholino-nitroalkenes, and aromatic aldehydes in the c

185 xtending this logic, we have chosen to study morpholino nucleic acid (MoNA), because the secondary am

186 d (MoNA), because the secondary amine of the morpholino-nucleotides is expected to be highly nucleoph

187 Neutralization by morpholinos of Ptena, but not of Ptenb, phenocopied the

188 rdiac trabeculation; (b) injection of gata1a morpholino oligomer (gata1aMO) suppressing hematopoiesis

189 transporter, Vivo, can effectively carry the Morpholino oligomer (MO) across the chorion, enter the e

190 ely affected SMA mice by delivering low-dose morpholino oligomer (PMO25) into the existing severe SMA

191 Here we show that an antisense morpholino oligomer directed against the exon 13-intron

192 /6 primary myotubes using phosphorodiamidate morpholino oligomer or locked nucleic acids (LNA)/2'-OMe

193 Zebrafish embryos injected with an elavl1 morpholino oligomer or miR-200b mimic showed angiogenesi

194 In addition, miR-200b and HuR morpholino oligomer suppressed the activity of a zVEGF 3

195 nding protein or changing its composition to morpholino oligomer that does not interact with helicase

196 AVI-7288 is a phosphorodiamidate morpholino oligomer with positive charges that targets t

197 isense peptide-conjugated phosphorodiamidate morpholino oligomer, T-ex5, that interferes with splicin

198 l genome such as modified phosphorodiamidate morpholino oligomer-based compounds and small interferin

199 re we present evidence for successful use of morpholino oligomers (MO) to mediate degradation of targ

200 tide-mediated delivery of phosphorodiamidate morpholino oligomers (PMOs) has shown great promise for

201 that co-administration of phosphorodiamidate morpholino oligomers (PMOs) with glucose enhances exon-s

202 Here, we focus on phosphorodiamidate morpholino oligomers (PMOs), ASOs that are especially st

203 Based on experiments with antisense morpholino oligomers as well as pharmacological agonists

204 Knockdown of mat2aa in zebrafish via morpholino oligomers disrupted cardiovascular developmen

205 y using splice modulating phosphorodiamidate morpholino oligomers to enhance GAA exon 2 inclusion in

206 Treatment of mdx mice with morpholino oligomers to induce exon skipping of dystroph

207 pression of gene expression, typically using Morpholino oligomers.

208 es and knockdown of PKCdelta expression with morpholinos oligomers inhibited TRPC1-based SOCs.

209 By contrast, blocking ASPN function with a morpholino oligonucleotide (ASPN-MO) inhibits eye format

210 Disruption of rab5ab expression by antisense morpholino oligonucleotide (MO) knockdown abolishes noda

211 of two nanoconjugates: (1) a single-stranded morpholino oligonucleotide (MORF1) attached to an anti-C

212 be the conjugation of two phosphorodiamidate morpholino oligonucleotide (PMO) SSOs to a single CPP fo

213 eptor CD20 (anti-CD20 Fab' conjugated with a morpholino oligonucleotide 1) and a CD20 clustering actu

214 rafted with multiple copies of complementary morpholino oligonucleotide 2).

215 f 2 mutation-specific antisense molecules (a morpholino oligonucleotide [MO] and an engineered U7 sma

216 e silencing of gGlcT1 synthesis by antisense morpholino oligonucleotide abolished ESV production and

217 form of ILK or by injecting an ILK antisense morpholino oligonucleotide.

218 Knockdown of scube1 by morpholino-oligonucleotide down-regulated the expression

219 analyzed its function by injecting antisense morpholino-oligonucleotide into embryos.

220 Spectrally differentiated caged morpholino oligonucleotides (cMOs) and wavelength-select

221 Reduction of blood viscosity via gata1a morpholino oligonucleotides (MO) reduced shear stress, r

222 For over 15 years, antisense morpholino oligonucleotides (MOs) have allowed developme

223 ximal PASs and its inhibition with antisense morpholino oligonucleotides (U1 AMO) triggers widespread

224 iption activator-like effector nucleases and morpholino oligonucleotides confirmed that the ecl mutan

225 Knock-down experiments using antisense morpholino oligonucleotides directed against hesx1 and f

226 ckdown of lurap1, p190RhoGEF and Golgin45 by morpholino oligonucleotides disrupts dystrophin localiza

227 Knockdown of amigo1 expression using morpholino oligonucleotides impairs the formation of fas

228 n of downstream intron splicing by antisense morpholino oligonucleotides inhibited NMD and rescued th

229 Knockdown of msmb3 with antisense morpholino oligonucleotides or disruption of msmb3 by CR

230 ppression of camk2g1 expression by antisense morpholino oligonucleotides or inhibition of CaMK-II act

231 Knockdown of Dhrs3 by antisense morpholino oligonucleotides resulted in a phenotype of s

232 , knockdown of zebrafish scube3 by antisense morpholino oligonucleotides specifically suppressed the

233 f72 was performed using 2 specific antisense morpholino oligonucleotides to block transcription.

234 aper, we have used small interfering RNAs or morpholino oligonucleotides to deplete the LICs in human

235 We used antisense morpholino oligonucleotides to target the orthologous Da

236 Antisense morpholino oligonucleotides were targeted to Ct-Smad2/3

237 cloning, insertional mutagenesis, antisense morpholino oligonucleotides, targeted re-sequencing, and

238 ent was validated in zebrafish embryos using morpholino oligonucleotides.

239 Knockdown of IoDpp by morpholino oligos prevents the development of all struct

240 Depletion with morpholino oligos showed that this extracellular gradien

241 In vivo blockade of YAP/TAZ translation by morpholino oligos significantly reduced endothelial infl

242 Using splice site-specific antisense morpholino oligos, we inhibited n1-src splicing, while p

243 ation of the effects of translation-blocking morpholinos on the innate immune response.

244 tion assays (via microinjection of antisense morpholino or CRISPR-Cas9) confirm that AChE is specific

245 the co-injection of ror2-TM mRNA and a wnt11 morpholino or the coexpression of ror2 and wnt11 in zebr

246 rgized with coexpressed Xwnt8, whereas Ripk4 morpholinos or catalytic inactive Ripk4 antagonized Wnt

247 CRISPR-based gene editing, RNA interference, morpholinos or pharmacological inhibition) can have a ma

248 Here we use morpholino- or dominant negative-mediated interference t

249 that the ability of such indels to suppress morpholino phenotypes does depend on the size and the lo

250 ating peptide and show that Pip6a-conjugated morpholino phosphorodiamidate oligomer (PMO) dramaticall

251 t an advanced peptide-oligonucleotide, Pip6a-morpholino phosphorodiamidate oligomer (PMO), which demo

252 Phosphorodiamidate morpholinos (PMOs) and PMO-DNA chimeras have been prepar

253 ingle dose of the peptide-phosphorodiamidate morpholino (PPMO) antisense oligonucleotides that induce

254 wn of zebrafish otg using specific antisense morpholino promoted nuclear accumulation of beta-catenin

255 tional inhibition using a 'sponge' vector or morpholinos promotes premature neural crest delamination

256 We study Cu(II) FSCV responses in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer and charac

257 horothioates (pS) and morpholinos, to create morpholino-pS hybrid oligonucleotides.

258 s strategy enables the easy incorporation of morpholino-pS moieties and therapeutically relevant suga

259 ribe the identification of a novel series of morpholino-pyrimidine DprE1 inhibitors.

260 in zebrafish embryos using a splice-blocking morpholino (rad21a).

261 ontent, including providing full support for morpholino reagents, used to inhibit mRNA translation or

262 ed within a MO target site would result in a Morpholino-refractive allele thus suppressing true MO ph

263 s of function of beta-catenin with antisense morpholinos reproducibly reduced the expression of 247 m

264 Knockdown of Elavl1a using specific morpholinos resulted in a striking loss of primitive emb

265 targeting cish.a, but not cish.b or control morpholinos, resulted in enhanced embryonic erythropoies

266 fish, using 2 different splice-site blocking morpholinos, resulted in larvae with tail curling and dy

267 Depletion of keratin (krt8) with antisense morpholinos results in high traction stresses in followe

268 ocking galanin expression with specific vivo-morpholino sequences inhibited hyperplastic cholangiocyt

269 ciliogenesis pathway and a scrambled control morpholino showed no phenotypic effect.

270 t zebrafish and zebrafish injected with ocrl morpholino showed truncated expression of megalin along

271 in a vasculogenesis assay in vivo by using a morpholino strategy in zebrafish.

272 ing the charge of the excluded strand with a morpholino substrate instead of DNA also dramatically in

273 es, phenotypes, genotypes, gene expressions, morpholinos, TALENs, CRISPRs, antibodies, anatomical str

274 Injection of morpholinos targeting cish.a, but not cish.b or control

275 Morpholinos targeting dyx1c1 in zebrafish also caused la

276 Morpholinos targeting two other guanine nucleotide excha

277 y, we determined that reduced expression via morpholino technologies of a single histone-modifying en

278 Blocking this signal with morpholino technology or silencing of the polyadenylatio

279 enable rapid and efficient extension of the morpholino-terminated primer on homopolymeric and mixed-

280 Here, we show that an antisense morpholino that base-pairs to the 5' end of U1 snRNA blo

281 Moreover, a customized Vivo-Morpholino that targets human GLS mRNA markedly inhibite

282 fine gene function in zebrafish, after which Morpholinos that recapitulate respective phenotypes coul

283 in zebrafish embryos injected with antisense morpholinos that targeted zebrafish st3gal5 expression.

284 cleotide analogue called thiophosphoramidate morpholinos (TMOs).

285 Using zebrafish morpholino to evaluate loss of function, we observed a s

286 tated human TNNT1 mRNAs were coinjected with morpholinos to assess their abilities to rescue the morp

287 If the animal caps carried morpholinos to either hox11/13b or foxA (endomesoderm sp

288 e for these candidate genes in vivo, we used morpholinos to reduce SYNE1, NUP37, and NUP43 gene expre

289 cophores, namely, phosphorothioates (pS) and morpholinos, to create morpholino-pS hybrid oligonucleot

290 pe and the skeletal defects observed in pkd1-morpholino treated fish.

291 ral pathfinding in Fzd3 mutant mice and Fzd3 morpholino treated frogs and found aberrant central proj

292 rategy in which mCARM1 was introduced in the morpholino-treated embryos exhibited recovery of the sen

293 In this method, a vivo morpholino (VMO) piggybacks an antisense deoxyoligonucle

294 A zebrafish loss-of-function model using morpholinos was created to assess the pathogenicity of t

295 Knockdown of Glis1 using morpholinos was performed in zebrafish animals 72 hours

296 , with the help of CARM1 inhibitor and CARM1 morpholinos, we show that inhibition of H3R17 methylatio

297 still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting tha

298 groups using inhibition by Dkk1 mRNA or Wnt8 morpholinos, which indicates that the effects of beta-ca

299 l (MOE) with a phosphorothioate backbone and morpholino with a phosphorodiamidate backbone-with the s

300 rad21a Morpholino zebrafish had delayed intestinal transit and