ライフサイエンスコーパス: multilineage

1 c colonies but also engraft and reconstitute multilineage adult blood.

2 ell can promote prolonged and high levels of multilineage allogeneic chimerism and robust tolerance t

3 se to markedly reduced numbers of definitive multilineage and lineage-committed hematopoietic progeni

4 initive hematopoietic stem cells, capable of multilineage and long-term reconstitution when transplan

5 Without hematopoietic stress, multilineage and myelo-erythroid bone marrow progenitor

6 e, although engrafted edited clones preserve multilineage and self-renewing capacity.

7 Donor chimerism was durable, multilineage, and stable.

8 lso reflect the likelihood that, in PBC, the multilineage antimitochondrial response is a pathogenic

9 nd regional lymph node than in blood, so the multilineage antimitochondrial response may be required

10 The results in other multilineage autoimmune cytopenia cohorts were encouragi

11 es in a majority of children with refractory multilineage autoimmune cytopenias.

12 al line ) SC from the same patients produced multilineage BCR/ABL1(-) engraftment.

13 nly hematopoietic cells capable of lifelong, multilineage blood cell production, understanding the st

14 of hematopoietic stem cells, but its role in multilineage blood development remains unclear.

15 unctional HSCs, which are fully competent in multilineage blood reconstitution upon transplantation.

16 ain transplantable HSCs capable of long-term multilineage blood reconstitution.

17 Conditional deletion of BAF53a resulted in multilineage BM failure, aplastic anemia, and rapid leth

18 ng GFP(+) BM cells showed long-term, serial, multilineage BM reconstitution, fulfilling the functiona

19 of E2F1 in the formation and maintenance of multilineage brain tumors, irrefutably establishing E2F1

20 erythroid colonies in vitro while retaining multilineage capacity, capable of generating MCs and oth

21 merization produced divisions of clonogenic, multilineage CD34(+) cells able to engraft immunodeficie

22 ed from pneumococcal surface adhesin A, is a multilineage cell activator in vitro.

23 regulators such as GATA2, ERG, and RUNX1 in multilineage cells and the lineage-specific master regul

24 itors (PPEPs)] has the potential to generate multilineage cells during normal development in vivo.

25 ychromatic flow cytometry was used to assess multilineage chimerism and lymphocyte recovery posttrans

26 A transient, low-to-moderate degrees of multilineage chimerism was observed after DBM infusion.

27 Fifteen patients developed multilineage chimerism without graft-versus-host disease

28 ong 30 recipients who successfully developed multilineage chimerism, 10 achieved long-term immunosupp

29 Multilineage chimerism, clonal deletion, and lymphocyte

30 mg/kg with CD154 blockade regularly induced multilineage chimerism.

31 eatment with eltrombopag was associated with multilineage clinical responses in some patients with re

32 oliferative neoplasms (MPN) characterized by multilineage clonal hematopoiesis.

33 f-renew, and their ability to give rise to a multilineage clone of differentiating and maturing blood

34 at 1 month but were supplanted over time by multilineage clones, initially myeloid restricted, then

35 transplantable, donor-derived, macroscopic, multilineage colonies detectable on the spleen surface 1

36 eural crest-derived cells capable of forming multilineage colonies in culture, and neurons and glia u

37 netically manipulated, and are competent for multilineage colonisation of chimaeras.

38 ematopoietic cells, accompanied by increased multilineage colony-forming potential in colony-forming

39 able for up to 1 year in vitro, and remained multilineage competent throughout.

40 Dox withdrawal in combination with multilineage cytokines restored GATA1 expression, result

41 SC accompanied by development of progressive multilineage cytopenias and cytological dysplasia.

42 ic deletion of Asxl1 results in progressive, multilineage cytopenias and dysplasia in the context of

43 Patients with autoimmune multilineage cytopenias are often refractory to standard

44 Patients often present with chronic multilineage cytopenias due to autoimmune peripheral des

45 We also treated 12 patients with multilineage cytopenias secondary to common variable imm

46 onset chronic lymphadenopathy, splenomegaly, multilineage cytopenias, and an increased risk of B-cell

47 denopathy, hepatosplenomegaly, and recurring multilineage cytopenias.

48 ostic evaluation of children with refractory multilineage cytopenias.

49 rogation of hematopoiesis as demonstrated by multilineage defects in lymphocyte, granulocyte, erythro

50 d whether HSC gene therapy could correct the multilineage defects seen in SAP(-/-) mice.

51 ential, but exhibited a fivefold decrease in multilineage definitive hematopoietic potential.

52 onstrated by the engraftment and recovery of multilineage descendants of CD33-ablated HSPCs.

53 lator of HSC self-renewal, mobilization, and multilineage development and executes these actions via

54 cells with diverse angiogenic functions and multilineage developmental features of mesenchymal stem

55 the numbers of haematopoietic stem cells and multilineage-differentiated cells are reduced in iqcg-de

56 tes the ability to effectively differentiate multilineage-differentiating stress-enduring cells into

57 Multilineage-differentiating stress-enduring cells were

58 absence of CHIR99021 and valproic acid, and multilineage differentiation ability is preserved.

59 nd exhibited clonogenic capacity, as well as multilineage differentiation ability.

60 oblast cells, which possess the capacity for multilineage differentiation and are responsible for pla

61 these challenges, due to their capacity for multilineage differentiation and indefinite proliferatio

62 ncer tissues is largely explained by in vivo multilineage differentiation and not only by clonal gene

63 ing holds promise for combining the accurate multilineage differentiation and physiology of in vivo s

64 These cells have potential for multilineage differentiation and provide a resource for

65 Thus, HSC maintenance and multilineage differentiation are distinct cell lineage d

66 graft experiment indicated that renewal and multilineage differentiation are normal in the absence o

67 different gene-expression programs linked to multilineage differentiation are strongly associated wit

68 SC) fate decisions between proliferation and multilineage differentiation are unclear.

69 and retain the capacity for self-renewal and multilineage differentiation as long as they reside in t

70 a single-cell sorting approach and in vitro multilineage differentiation assays, here we show that i

71 s colonize embryonic germ layers and exhibit multilineage differentiation both in fetal and adult chi

72 r results reveal that Mbd3/NuRD can regulate multilineage differentiation by constraining the activat

73 ll surface extracellular matrix receptors in multilineage differentiation by these multipotent human

74 feration, resistance to oxidative stress and multilineage differentiation capacities compared with ag

75 rthermore, these cells showed clonogenic and multilineage differentiation capacities.

76 expression and significantly improved their multilineage differentiation capacities.

77 To investigate the multilineage differentiation capacity of these stem cell

78 able to produce HAP stem cell colonies with multilineage differentiation capacity.

79 ation, indicating that competence to undergo multilineage differentiation depends upon JAK/STAT.

80 onment to fulfill long-term self-renewal and multilineage differentiation functions, an event that is

81 ) complex is required for maintenance of and multilineage differentiation in the early hematopoietic

82 and genetic manipulation of cells capable of multilineage differentiation in vitro and in vivo to exa

83 sFRP2-MSCs maintained their ability for multilineage differentiation in vitro and, when implante

84 nated control of stem cell proliferation and multilineage differentiation is essential to ensure a st

85 s neurofibromin-regulated NSC proliferation, multilineage differentiation is MEK-dependent.

86 aftment in the bone marrow of the hosts, and multilineage differentiation of hematopoietic cells was

87 o culture without altering proliferation and multilineage differentiation of HSC and progeny.

88 hat supports robust expansion and subsequent multilineage differentiation of human pluripotent stem c

89 f human stem cells from the PDL and gingiva, multilineage differentiation of those cells, and compari

90 lls (MSCs) from hESCs (hESCd-MSCs) that have multilineage differentiation potential and are capable o

91 ed regeneration therapies by virtue of their multilineage differentiation potential and immunogenicit

92 roliferative multipotent cells with in vitro multilineage differentiation potential and in vivo engra

93 quire long-term self-renewing capacities and multilineage differentiation potential during physiologi

94 proliferating progenitor cells, and possess multilineage differentiation potential generating functi

95 ically similar to bone marrow HSPCs and have multilineage differentiation potential in vitro and in v

96 cells exhibited long-term proliferation and multilineage differentiation potential in vitro.

97 a non-self-renewing progenitor with limited multilineage differentiation potential termed the erythr

98 ionally heterogeneous, and displays distinct multilineage differentiation potential, cell cycle profi

99 id not adversely impact either clonogenic or multilineage differentiation potential, indicating a sel

100 ignated induced-HSCs (iHSCs), possess clonal multilineage differentiation potential, reconstitute ste

101 mechanisms to engineer progenitor cells with multilineage differentiation potential.

102 gy, typical hMSC marker profile and in vitro multilineage differentiation potential.

103 /progenitor-like cells have self-renewal and multilineage differentiation potential.

104 including the capacity for self-renewal and multilineage differentiation potential.

105 vitro CD34(+) cell derived SG-MSCs revealed multilineage differentiation potential.

106 s cellular heterogeneity truly recapitulates multilineage differentiation processes of normal tissues

107 Moreover, whereas MEK-regulated multilineage differentiation requires Smad3-induced Jagg

108 nd contains tissue-derived MSCs that exhibit multilineage differentiation similar to bone marrow-deri

109 ols neural stem cell (NSC) proliferation and multilineage differentiation through the selective use o

110 hermore, this population demonstrates robust multilineage differentiation using standard in vivo and

111 Multilineage differentiation within clones was demonstra

112 are uniquely able to self-renew, to undergo multilineage differentiation, and to persist throughout

113 the competence of daughter cells to undergo multilineage differentiation, ensuring a robust cellular

114 nt of cell therapies and tissue engineering: multilineage differentiation, immunomodulation, and prod

115 long with the properties of self-renewal and multilineage differentiation, pancreatic CSCs display up

116 GPx2 overexpression stimulated multilineage differentiation, proliferation, and tumor g

117 rther explore the role of alpha2 integrin in multilineage differentiation, we established multipotent

118 maintain the HSCs' pool and the ability for multilineage differentiation, which are responsible for

119 roid mice are defective in proliferation and multilineage differentiation.

120 of individual RGLs for both self-renewal and multilineage differentiation.

121 sphere-like organoids with proliferation and multilineage differentiation.

122 d HCECs that are capable of self-renewal and multilineage differentiation.

123 al characteristics revealed-self-renewal and multilineage differentiation.

124 ET1 for 2 cell passages and then induced for multilineage differentiation.

125 ructure, pluripotency-marker expression, and multilineage differentiation.

126 es have the potential to improve iPSC-driven multilineage differentiation.

127 their ability to proliferate and to undergo multilineage differentiation.

128 basal cells capable of clonal expansion and multilineage differentiation.

129 reatment translates into increased long-term multilineage donor cell engraftment.

130 of alloengraftment remained enigmatic until multilineage donor leukocyte microchimerism was discover

131 the classification of CEBPA-mutated AML with multilineage dysplasia (MLD; >/= 50% dysplastic cells in

132 n DNA methylation genes were associated with multilineage dysplasia (P = .015) but had no effect on c

133 RS (RARS), 43 with refractory cytopenia with multilineage dysplasia (RCMD)-RS, 11 with refractory ane

134 n complex were independently associated with multilineage dysplasia and identified a distinct subset

135 subtypes, such as refractory cytopenia with multilineage dysplasia and ring sideroblasts (RCMD-RS).

136 Four patients had refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS)

137 ineage dysplasia, others have suggested that multilineage dysplasia correlates with unfavorable cytog

138 t SF3B1 and inversely with hemoglobin level, multilineage dysplasia, and high-risk karyotype; but did

139 ory anemia, n = 1; refractory cytopenia with multilineage dysplasia, n = 1) had no UPD4q24.

140 ukemia, n = 1; and refractory cytopenia with multilineage dysplasia, n = 1).

141 leukemia (AML), including the importance of multilineage dysplasia, others have suggested that multi

142 These genome-edited HSPCs support multilineage engraftment and generate progeny capable of

143 Long-term multilineage engraftment and persistent VCN and vector e

144 Conditioning with CD45-SAP allows multilineage engraftment and robust immune reconstitutio

145 mitted greater expansion of cells capable of multilineage engraftment and serial transplantation, hal

146 Primary and secondary recipients showed multilineage engraftment at 3 months after transplantati

147 ematopoietic cells with robust and sustained multilineage engraftment has not been achieved.

148 these findings, we achieved stable long-term multilineage engraftment in 21 of 24 surviving recipient

149 tioning with CD45-SAP enabled high levels of multilineage engraftment in both Rag1 mutant models, all

150 They were also able to give rise to multilineage engraftment in both secondary recipients an

151 cobblestone area-forming cell potential, and multilineage engraftment in NOD/SCID/IL2R-gamma(null) mi

152 tudy demonstrated clear safety with durable, multilineage engraftment of dmPGE2-treated UCB units.

153 Long-term multilineage engraftment of Klf7(-/-) cells was comparab

154 ow-grade acute toxic effects and resulted in multilineage engraftment of transduced cells, reconstitu

155 Rapid hematopoietic recovery with polyclonal multilineage engraftment of vector-marked cells was achi

156 how that fluid shear stress endows long-term multilineage engraftment potential upon early hematopoie

157 matopoietic stem cells capable of long-term, multilineage engraftment still remains a significant cha

158 Our aim was to improve multilineage engraftment; we tested nongenotoxic conditi

159 s expressing stem cell markers and retaining multilineage epithelial differentiation capability.

160 to human NPMc+ AML, where the observed NPMc+ multilineage expression pattern implies transformation o

161 hat Gimap5-deficient mice showed progressive multilineage failure of bone marrow and hematopoiesis.

162 , which successfully engraft and expand in a multilineage fashion in secondary transplantation recipi

163 ed expression of KLF7, although resulting in multilineage growth suppression that extended to hematop

164 Multilineage hematopoiesis from anti-HIV lentiviral vect

165 r of SCID-repopulating cells and established multilineage hematopoiesis in primary and secondary immu

166 ent that could radioprotect and reconstitute multilineage hematopoiesis in secondary and tertiary rec

167 In contrast, we observed multilineage hematopoiesis up to 68 weeks after primary

168 efficiently self-renew and sustain long-term multilineage hematopoiesis upon serial transplantation.

169 the major contributor to the maintenance of multilineage hematopoiesis, both in the steady state and

170 Cs during cytokinesis and thus essential for multilineage hematopoiesis.

171 phoid tissue that demonstrate high levels of multilineage hematopoiesis.

172 ss" phase and develop to fertile adults with multilineage hematopoiesis.

173 al to HSC can result in a profound effect on multilineage hematopoiesis.

174 ulture-initiating cell potential and sustain multilineage hematopoietic cell engraftment when transpl

175 We now report on transient multilineage hematopoietic chimerism and lymphocyte reco

176 transient inflammasome stimulation increased multilineage hematopoietic colony-forming units and T ce

177 ch deficient emi1 gene expression results in multilineage hematopoietic defects and widespread develo

178 CD41-knockout (KO) mice displayed multilineage hematopoietic defects coupled with decrease

179 granulocyte lineage commitment to facilitate multilineage hematopoietic differentiation and thus iden

180 erized by exocrine pancreatic insufficiency, multilineage hematopoietic dysfunction, and metaphyseal

181 >40-80 population doublings, are capable of multilineage hematopoietic engraftment of immunodeficien

182 model, we show that RhoA deficiency causes a multilineage hematopoietic failure that is associated wi

183 This multilineage hematopoietic failure was rescued by recons

184 strate, on a clonal level, that they exhibit multilineage hematopoietic potential.

185 During developmental hematopoiesis, multilineage hematopoietic progenitors are thought to de

186 sess >/=9-week repopulation ability and show multilineage hematopoietic reconstitution in vivo.

187 ting target for promoting HSPC expansion and multilineage hematopoietic recovery after transplant.

188 potentially superior, approaches to promote multilineage hematopoietic recovery by blocking the TGFb

189 hereas the CD34(+) fraction contained normal multilineage hematopoietic repopulating cells.

190 odeficient mice provide long-term high level multilineage hematopoietic repopulation.

191 g and transcriptional circuits that regulate multilineage hematopoietic specification.

192 ty to engraft and to repopulate a functional multilineage hematopoietic system in vivo.

193 This protocol resulted in multilineage, high level (up to 50%), durable, donor-der

194 During embryonic development, multilineage HSCs/progenitor cells are derived from spec

195 ular endothelial cells and the generation of multilineage HSPCs from hemogenic endothelium.

196 mouse strain, we have established long-term multilineage human grafts and demonstrated their seriall

197 an immune system have a capacity for de novo multilineage human hematopoiesis and generate T cells, B

198 mmac(-/-) mice results in the development of multilineage human hematopoietic cells.

199 f CD34(+) cells from the same donor achieves multilineage human lymphohematopoietic reconstitution, i

200 helming female predominance, production of a multilineage immune response to mitochondrial autoantige

201 First, the multilineage immune response, including AMAs, is directe

202 Our multilineage immunohistochemical analyses of early embry

203 hanisms of immune regulation; and subsequent multilineage immunopathology impacts upon uniquely susce

204 drove the recovery of human HSCs capable of multilineage in vivo repopulation following radiation in

205 es the regeneration of human HSCs capable of multilineage in vivo repopulation.

206 or studying gene functions simultaneously in multilineages in the lung.

207 C) for immune cell subsets revealed a marked multilineage increase in infiltrates, consisting predomi

208 Multilineage involvement of bone marrow (BM) hematopoies

209 The multilineage involvement of the BCR-ABL1-positive clone

210 BM mast cells (64% vs 0%; P = .01) vs other multilineage ISM cases.

211 Multilineage KIT involvement and multimutated clones are

212 All MSC-mutated patients had multilineage KIT mutation (100% vs 30%, P = .0001) and t

213 ervations such as cellular reprogramming and multilineage locus priming.

214 ones, and marrow from ossicles reconstituted multilineage long-term hematopoiesis in lethally irradia

215 yeloid-B clones, and then stable myeloid-B-T multilineage, long-term repopulating clones.

216 h minimal conditioning led to stable, mixed, multilineage lymphoid and myeloid macrochimerism, deleti

217 hPSC-derived lung progenitors (LPs) undergo multilineage maturation into proximal cells, type I alve

218 se findings establish an approach to achieve multilineage maturation of lung and airway cells from hP

219 With multilineage mesodermal potential and possible ectoderma

220 Transient multilineage mixed chimerism was observed in all patient

221 These results characterize the transient multilineage mixed hematopoietic chimerism and recovery

222 isease is characterized by severe anemia and multilineage myeloid dysplasia that are thought to be a

223 ndings establish an intrinsic disturbance of multilineage myeloid hematopoiesis in trisomy 21 at the

224 two types of neuronal progenitors (NPs) from multilineage-negative NSCs.

225 d T(H) signatures that suggested overlapping multilineage phenotypes with pro- and anti-inflammatory

226 eural progenitor cells with self-renewal and multilineage potency.

227 ously uncharacterized roles in promoting NSC multilineage potential and modulating early neural fate

228 at adult hippocampal NSCs inherently possess multilineage potential but that Drosha functions as a mo

229 A9, ERG, and RORA conferred self-renewal and multilineage potential in vitro and maintained primitive

230 SPCs) robustly proliferate while maintaining multilineage potential in vivo; however, an incomplete u

231 The persistence of fetal stem cells with multilineage potential in women who have been pregnant,

232 Priming and resolution of multilineage potential is dependent on the activity of t

233 c transplantation experiments to assess true multilineage potential of engrafted cells.

234 Recent studies show that the fate and multilineage potential of epithelial stem cells can chan

235 transcriptional regulators necessary for HSC multilineage potential to be maintained.

236 t do conform to classical definitions retain multilineage potential, but surprisingly, cannot make B

237 by resistance to chemotherapy, self-renewal, multilineage potential, increased colony formation, and

238 ategies have generated progenitor cells with multilineage potential, to date, therapy-grade engineere

239 one genome editing with CRISPR/Cas9 retained multilineage potential.

240 icle bulge are self-renewing stem cells with multilineage potential.

241 progenitor cells in the gastric antrum have multilineage potential.

242 and investigated whether these cells possess multilineage potential.

243 re subpopulation of gastric progenitors with multilineage potential.

244 poietic cells that lack robust and sustained multilineage potential.

245 ntain long-term growth of genomically stable multilineage pre-stasis HMEC populations can greatly enh

246 hese regulatory genes were also monitored in multilineage precursors as they entered T-cell or non-T-

247 e cell types representing a progression from multilineage precursors to differentiated erythroblasts

248 and pharynx adopt their terminal fates; (ii) multilineage priming contributes to the differentiation

249 ation and commitment states characterized by multilineage priming followed by gradual T cell commitme

250 Thus, in hematopoietic progenitors, multilineage priming of enhancer elements precedes commi

251 Although this multilineage priming was resolved upon subsequent lineag

252 th regulators, explaining the phenomenon of "multilineage priming".

253 development of LTi cells did not go through multilineage priming.

254 initial organogenesis involves a process of multilineage priming.

255 These data support a luminal multilineage progenitor cell model for prostate tissue a

256 xpressing primitive HSPCs capable of forming multilineage progenitor colonies (colony-forming units [

257 ) myeloid precursors derived from hPSCs into multilineage progenitors that can be expanded in vitro a

258 In multilineage progenitors, the likely target genes are en

259 tenin active cells and inhibits expansion of multilineage progenitors.

260 lly; however, we identified defects in their multilineage reconstituting capacity.

261 istatin enriches for long-term hematopoietic multilineage reconstituting cells by 5-fold or more as a

262 assays, Tet2-deficient HSCs were capable of multilineage reconstitution and possessed a competitive

263 matopoietic stem cell (HSC) self-renewal and multilineage reconstitution are controlled by positive a

264 ation; however, transplantation efficacy and multilineage reconstitution can be limited by inadequate

265 ion induced both defects in self-renewal and multilineage reconstitution in cyclin E knock-in HSCs wi

266 from 5-fluorouracil treatment and diminished multilineage reconstitution in lethally irradiated bone

267 f transplantable HSCs that provided balanced multilineage reconstitution in primary and secondary mou

268 rogenetic) derived cells conveyed long-term, multilineage reconstitution of hematopoiesis in recipien

269 -catulin-GFP(+)c-kit(+) cells give long-term multilineage reconstitution of irradiated mice, indicati

270 n vivo (as shown by the successful long-term multilineage reconstitution of primary neonates and seco

271 tituting adult progenitors to give long-term multilineage reconstitution that resembled fetal hematop

272 hich endows them with the ability to mediate multilineage reconstitution that resembles fetal lymphop

273 etic stem cell (HSC) that supports long-term multilineage reconstitution upon transplantation into ad

274 M cells yields HSC enrichments and long-term multilineage reconstitution when transferred to lethally

275 l products are capable of stable, polyclonal multilineage reconstitution with follow-up of more than

276 mpete with BM from wild-type mice to provide multilineage reconstitution, indicating that there is an

277 of HSCs and early HPCs that are defective in multilineage reconstitution, suggesting a differentiatio

278 ained upon serial transplantation, with full multilineage reconstitution.

279 try demonstrate that damaged lobes underwent multilineage regeneration, reforming a lobe often indist

280 Finally, base edits could be produced in multilineage-repopulating self-renewing human HSCs with

281 ncluding self-renewal capacity and long-term multilineage repopulation ability.

282 nome-edited HSPCs engraft, and contribute to multilineage repopulation after autologous transplantati

283 The SALL4 expanded HSCs/HPCs retain multilineage repopulation and long-term engraftment acti

284 posed to EPCR-negative cells, exhibit robust multilineage repopulation and serial reconstitution abil

285 geted integration in human HSCs by long-term multilineage repopulation of transplanted mice.

286 Gene-modified cells achieved multilineage repopulation, and we identified the same ge

287 marrow cells resulted in defective long-term multilineage repopulation.

288 biliary cirrhosis (PBC), patients develop a multilineage response to a highly restricted peptide of

289 to immunosuppressive therapy, with frequent multilineage responses and maintenance of normalized hem

290 in the youngest subject (age 10 years), and multilineage retroviral marking occurred in all 3 childr

291 port efficient and stable MGMTP140K-mediated multilineage selection in both macaque and baboon nonhum

292 ork for modeling complex processes involving multilineage specifications.

293 ge colony-stimulating factor (GMCSF) induced multilineage, STAT5-dependent differentiation, including

294 y enhanced by the rigorous dissection of the multilineage T and B cell response against the immunodom

295 PGCs are also the cell of origin of multilineage teratocarcinomas.

296 complex process resulting in an integrated, multilineage tissue with developmental corruption in ear

297 Multilineage transcriptional priming is quickly resolved

298 1, Hlf, Lmo2, Prdm5, Pbx1, and Zfp37 imparts multilineage transplantation potential onto otherwise co

299 em cell function, tumor-initiating cells and multilineage tumor development.

300 We show that transgenic mice developed multilineage tumors, including metastatic SCCs.