ライフサイエンスコーパス: multiplex

1 Here, we introduce the concept of azimuthal multiplexing.

2 tep in the realization of OAM modulation and multiplexing.

3 need for multiple vectors, and challenges in multiplexing.

4 inatorial gene circuit tests, and for CRISPR multiplexing.

5 rm one-dimensional sensor barcodes for rapid multiplexing.

6 ble luminescence as a new option for optical multiplexing.

7 rototype and an 8-channel frequency-division multiplexed (0.5 m x 1,000 bps) prototype are demonstrat

8 As a case study, we multiplexed 3 carbapenem-resistant genes to show the imp

9 s the limitations and potential of azimuthal multiplexing 3D diffractive optics.

10 d and characterized the performance of MASQ (multiplex accurate sensitive quantitation), a method wit

11 Multiplexing across donors is a popular experimental des

12 onformation capture (Hi-C) analysis revealed multiplex, activated enhancer-promoter configurations en

13 Products were evaluated by multiplexed activity profiling (MAP), an unbiased platfo

14 We developed CaRMA (calcium and RNA multiplexed activity) imaging based on recording in vivo

15 We combine it with Cell Hashing to further multiplex additional experimental conditions, such as po

16 Multiplexed AED measurements using LC-MS/MS and LC-DTIM-

17 profiling with structure library (FOREST), a multiplexed affinity assay system to identify functional

18 A multiplex allele-specific PCR targeting outbreak-specifi

19 Cytokine secretion was measured by multiplex analysis and ELISA.

20 overlap of these commonly used fluorophores, multiplex analysis becomes limited.

21 ary melanoma by high-dimensional single-cell multiplex analysis in tissue sections (MILAN technique)

22 udy, we developed an integrated workflow for multiplex analysis of global, glyco-, and phospho-proteo

23 into a microfluidic chip, achieving one-step multiplex analysis within 40 min.

24 athematically characterise which systems can multiplex and how well they can do it.

25 ave sufficient resolution and specificity to multiplex and quantify.

26 ssible implementations of this technique for multiplexed and high-throughput biosensing are discussed

27 We have developed a multiplexed and high-throughput method to quantify drug

28 rates, but finer temporal coding might allow multiplexing and enhanced readout across the connected n

29 The intrinsic multiplexing and throughput capabilities of CARMEN make

30 ate transducers that can be miniaturized and multiplexed, and that are easy to operate and cheap.

31 We developed a multiplexing approach using a three-color fluorescence s

32 ules (components, c-sIgE) was measured using multiplex array among 509 adults and 140 school-age and

33 A multiplex assay confirmed protein levels of specific cyt

34 However, limitations of existing multiplex assay methods in cultured mammalian cells hind

35 We aimed to develop a high-throughput multiplex assay to detect antibodies to SARS-CoV-2 to as

36 GCF were quantitated by sandwich ELISAs and Multiplex assay, respectively.

37 gonist) trial participants (n = 379) using a Multiplex assay.

38 Here, we report a multiplexed assay for the fluorescence-based detection o

39 Using CARMEN-Cas13, we developed a multiplexed assay that simultaneously differentiates all

40 ants was considered to investigate whether a multiplexed association was prevalent between nutrition,

41 lity to rank genes on whole genomes from 756 multiplex autism families.

42 The techniques that enable this, multiplex automated genome engineering (MAGE) and direct

43 eening, disruption of regulatory regions and multiplex base editing applications.

44 Our data demonstrate feasibility of multiplex base editing for polygenic disease modeling in

45 Optical multiplexing based on luminescent materials with tunable

46 hich represents a 19.7% increase compared to multiplexing based on the final fluorescent intensity.

47 We conclude that multiplex bead array assessment of non-HLA antibodies id

48 Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and

49 We analyzed specimens with a 62-cytokine multiplex bead-based enzyme-linked immunosorbent assay.

50 Real-time multiplexed bead tracking therefore requires a more effi

51 , and analyzed them for 33 cytokines using a multiplexed bead-based assay.

52 Herein, we describe a multiplexed bioluminescent repair reporter (BLRR) for no

53 ture development of this approach may enable multiplexed biomarker detection by using ribbons of DNA

54 application of high-resolution GCIB-SIMS for multiplexed biomolecular analysis at the level of single

55 tion, efficiency, and robustness can improve multiplexed biophysical bead-tracking applications, espe

56 The multiplex biosensor system with on-demand panel composit

57 The multiplexed biosensor showed a limit of detection of 0.2

58 The development of wearable multiplexed biosensors has been focused on systems to me

59 Compact multiplexed biosensors systems hold great potential for

60 promising alternative for the generation of multiplexed biosensors that can detect both protein and

61 lid state MALDI, as well as MS methods using multiplexing by labeling, which in principle can also be

62 Finally, we discuss the unique multiplexing capabilities of CRISPR screens to investiga

63 er milliliter) threefold faster, with higher multiplexing capacity and with a more straight-forward p

64 The multiplexing capacity of published IPTL implementations

65 SMD-IPTL extends the multiplexing capacity to 4-plex with the potential for h

66 by immunoassays; however, these have limited multiplexing capacity, are costly, and can exhibit cross

67 e overcoming autofluorescence and increasing multiplexing capacity.

68 low sensitivity, nonspecificity, and minimal multiplexing capacity.

69 r highly sensitive quantitative detection of multiplex cardiac biomarkers in human serum to expedite

70 Using this fluorescent multiplex cell rolling (FMCR) assay, each sample is labe

71 ectrometry have enabled the high-throughput, multiplexed chemical analysis of single cells, capable o

72 in other genetically tractable organisms for multiplexed conditional genome engineering.

73 eening limitations and present methods for a multiplex CRISPR/Cas9 haploid screen in chimeric axolotl

74 This highly efficient multiplex CRISPR/Cas9_Trex2 system makes it possible to

75 In this review, we discuss multiplexed CRISPR technologies and describe methods for

76 Multiplexed CRISPR technologies, in which numerous gRNAs

77 plement a total of 14 genes knockdowns using multiplex-CRISPRi.

78 We implemented a rapid multiplex cytokine assay to measure serum interleukin (I

79 0 strains, using parallel (DCyFIRscreen) and multiplex (DCyFIRplex) DCyFIR modes, recapitulated known

80 Multiplex detection of HPV16/18 in clinical human swab s

81 s (MNAzymes)-based system for singleplex and multiplex detection of NA targets in microwells down to

82 The developed technique can be used for multiplex detection of trace amounts of pathogens of var

83 the spleen of mice were analyzed by cytokine multiplex detection, qRT-PCR, and flow cytometry respect

84 The assay, which we show is amenable to multiplexed detection in a single lateral-flow strip inc

85 logically encode the fluorescence states for multiplexed detection of low-abundance targets.

86 he detection scheme and potentially enabling multiplexed detection.

87 c paper-based microfluidic platform for fast multiplexed detections of important biomarkers in variou

88 on of single-molecule recognition events and multiplexed discrimination of living cells.

89 n-nitride platform, we demonstrate a spatial-multiplexed DOPO system using continuous-wave pumping.

90 The hydrolysis probe-based multiplex dPCR assay quantifies SMN1, SMN2, and the tota

91 However, a higher-order multiplexing dPCR assay for measuring SMN1 and SMN2 copy

92 This approach is difficult to multiplex due to spectral overlap between any additional

93 itu imaging but faces limitations concerning multiplexing, dynamic range, and the required multiple w

94 locus editing to as high as 50% and improves multiplex editing by 5- to 10-fold in E. coli, while Pap

95 recombination processes has enabled precise, multiplex editing of microbial genomes and the construct

96 This system, named Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA)

97 m was constructed using a flexible thin-film multiplexed electrodes with b2LOxS and a DET-type glucos

98 n cell extracts were measured using Illumina multiplex Elisa and western blot, respectively.

99 tained for the MMP-8 and -9 determination by Multiplex Elisa Assays.

100 Herein, we present a highly scalable multiplexed ELISA that achieves a similar level of perfo

101 able examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed ima

102 develop Combinatorial Arrayed Reactions for Multiplexed Evaluation of Nucleic acids (CARMEN), a plat

103 High-resolution, multiplexed experiments are a staple in cellular imaging

104 ovides complete control over targeted sample multiplexing experiments, termed Tomahto, and present it

105 nstrating the utility of hiPSCs derived from multiplex families to identify significant cell-specific

106 Whole genome sequencing in multiplex families, proband-parent trios, and case-contr

107 ntal or comorbidity risk factors and studied multiplex family age-related hearing loss (mARHL) and si

108 eceptor ligation using pseudovirus entry and multiplexed Fc array assays, respectively.

109 While newly advanced multiplexed FISH imaging offers possibilities for refine

110 on of these methods is the complexity of the multiplexed FISH probe design.

111 robeDealer to design probes for a variety of multiplexed FISH techniques and their combinations.

112 filing, monosynaptic retrograde tracing, and multiplexed FISH to characterize the cells of the mouse

113 the performance of the BioPlex 2200 MMRV IgG multiplex flow immunoassay (MFI; Bio-Rad Laboratories, H

114 uidic single-bead trapping permits massively multiplexed fluorescence detection and quantification of

115 We present split-FISH, a multiplexed fluorescence in situ hybridization method th

116 We used multiplexed fluorescent immunohistochemistry and in situ

117 quantify all biomarkers in either single- or multiplex format.

118 Multiplexed gas detection at room temperature is critica

119 In addition, we achieve multiplexed gene activation with a crRNA array.

120 at a time, complex genetic diseases require multiplexed gene perturbations to interrogate combinatio

121 Multiplex genetic assays can simultaneously test thousan

122 Currently, either highly multiplexed genetic manipulations can be delivered to ma

123 guided endonucleases are promising tools for multiplexed genetic perturbations because they can proce

124 Here, we have developed multiplex genome editing toolkits for citrus including P

125 We have developed multiplex genome editing toolkits for citrus that signif

126 efficacy, and tRNA-mediated or Csy4-mediated multiplex genome editing.

127 n Cas9 single-guide RNA arrays, facilitating multiplex genome engineering.

128 With this construct, multiplex genomic editing of up to three loci was achiev

129 we measure the prevalence and energetics of multiplex H-bonds that are formed between three or more

130 esign is amenable to future integration into multiplexed high-throughput platforms for screening of m

131 Here, we present a multiplex human pluripotent stem cell (hPSC) platform, i

132 Using targeted tandem mass tags-based sample multiplexing, hundreds of proteins can be quantified acr

133 ) patients iteratively labeled with a highly multiplexed (hyperplexed) panel of 55 fluorescently tagg

134 hensive subtyping of influenza A strains and multiplexed identification of dozens of HIV drug-resista

135 iRFPs should become versatile NIR probes for multiplexed imaging across spatial scales in different m

136 d cyanine dyes offer spectral flexibility in multiplexed imaging experiments and enhanced precision i

137 By enabling multiplexed imaging of dopamine with other circuit compo

138 First we demonstrate multiplexed imaging of hundreds of genomic loci by seque

139 Precise multiplexed imaging of miRNA-155 and miRNA-21 cancer bio

140 acid (FNA) capture for sensitive, rapid, and multiplexed imaging of miRNAs cancer biomarkers in livin

141 RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), an

142 bled direct tracing of chromatin folding and multiplexed imaging of nucleome architectures in single

143 A new multiplex immune profiling panel (IPP) prototype was ass

144 ue circulating proteins were quantified by a multiplex immunoassay (Olink) and used to recapitulate s

145 amining peripheral inflammation in DLB using multiplex immunoassay and flow cytometry concomitantly.

146 s were identified from urine samples using a multiplex immunoassay or from positive blood cultures.

147 Ib/III) anti-GBS antibodies were measured by multiplex immunoassay prevaccination and 30/60 days post

148 We applied a multiplex immunoassay to serially collected saliva sampl

149 Using a multiplex immunoassay, we measured interleukin (IL) 1bet

150 cytokine concentrations were measured using multiplex immunoassay.

151 ific quantification of brain cytokines using multiplex immunoassays.

152 ing development of therapeutics.METHODSUsing multiplex immunohistochemistry, we characterized cerebro

153 Multiplexed immunohistochemistry was used to analyze exp

154 ingle-cell genomics technologies and spatial multiplex in situ methods now provide an opportunity to

155 at-Dx Respiratory Panel (QIAstat-Dx RP) is a multiplex in vitro diagnostic test for the qualitative d

156 ng genetic lineage tracing, clonal analysis, multiplexed in situ hybridization, immunostaining, deep

157 pulse-chase label retention experiments and multiplexed in situ hybridization, we identify a populat

158 classification to identify cell types using multiplexed in situ RNA detection.

159 The fluorescent sensors are subsequently multiplexed in the concavities of an engraved scleral le

160 ents can be exploited to perform data-driven multiplexing in a single fluorescent channel using machi

161 and wearable ion-to-electron transducers for multiplexed, in situ potentiometric analysis of pH, Na(+

162 design allows for increased flexibility and multiplexing, increased signal-to-noise, all without com

163 A multiplexed inflammatory protein analysis showed differe

164 that the NF-kappaB transcription factor can multiplex information about changes in multiple signals.

165 and link these methods to a new theory about multiplexing information.

166 This method clears a path for highly multiplexed investigations of the regulatory genome of m

167 gh-dimensional imaging approaches (including multiplexed ion beam imaging (MIBI) and imaging mass cyt

168 Combining our method with multiplexed ion beam imaging by time of flight (MIBI-TOF

169 Integration of multiplexed ion mobility scans is also shown to increase

170 Using a multiplexed isobaric labeling approach (TMT-11plex), ~77

171 ing a cell-based proteomics technique called multiplexed kinase inhibitor beads/mass spectrometry (MI

172 We report a multiplex label-free antigen microarray on the Arrayed I

173 A multiplex label-free biosensor is developed for diagnost

174 Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chro

175 Non-HLA antibodies were assessed using multiplex Luminex assay.

176 We formally define multiplexing, mathematically characterise which systems

177 on microscopy, corrosion casting, and direct multiplexed measurement of gene expression.

178 mise for multidimensional high-capacity data multiplexing, meeting the ever-increasing demands for in

179 Here, we improve DropSynth, a low-cost, multiplexed method that builds gene libraries by compart

180 We describe a universal sample multiplexing method for single-cell RNA sequencing in wh

181 Here we propose the multiplex model as a new perspective from which to under

182 ith immense potential has emerged, including multiplexed molecular panels directly applicable to bloo

183 e proteins, a significant limitation of many multiplex MS methods.

184 terized its advantages, including non-biased multiplex mutagenesis, over existing site-directed mutag

185 However, multiplexed NDIR gas sensing requires multiple pairs of

186 genome editing in C. elegans We developed a multiplexed negative selection screening approach in whi

187 In disease diagnostics, single- and multiplex nucleic acid (NA) detection, with the potentia

188 ification Gram-Positive (BCID-GP) Panel is a multiplex nucleic acid amplification assay based on comp

189 Vs were prospectively tested in 2394 NOPS by multiplex nucleic acid testing, including 1816 (75%) sim

190 Code-multiplexing of distributed Coulter sensors enables simp

191 tream targets may exploit such goal-directed multiplexing of VTA DA neurons to adjust actions to opti

192 Herein we demonstrate a rapid, multiplex, one-pot detection method based on two-step am

193 e of MURs can serve as a cocktail sensor for multiplex optical urinalysis in the mouse models of drug

194 context of a low cost, high throughput, and multiplexed optical imaging platform.

195 is, restricting longitudinal experiments and multiplexed outcome measures.

196 a new, rapid, and accurate sample-to-answer multiplex panel for the detection of the most common vir

197 eic acids (CARMEN), a platform for scalable, multiplexed pathogen detection.

198 Sputum multiplex PCR could become a useful diagnostic tool for

199 Multiplex PCR testing was also utilized for Ambler class

200 were screened for mcr-1 to mcr-5 genes by a multiplex PCR, and their genetic diversity was measured

201 replacing conventional stool testing with a multiplexed PCR assay, without an increase in testing co

202 Multiplexed polymerase chain reaction (PCR) assays incre

203 Georgia Institute of Technology, synthesized multiplexed primers and probes and formulated a master m

204 have been extensively applied in developing multiplex processing platforms to provide an accurate de

205 okine concentrations were determined using a multiplex protein bead assay.

206 Recent advances in transcriptomics, multiplex protein expression analysis, and experimental

207 gets and validate the possibility to further multiplex protein regulation via dual-wavelength optogen

208 pment of more sensitive and rapid methods of multiplexed protein analysis.

209 to 5 log-decades dynamic range for accurate, multiplexed protein determinations.

210 a novel method of manufacturing high-density multiplexed protein microarrays with the capacity to det

211 ay for development of useful meat industrial multiplex-proteins assays.

212 onse to infection utilizing an aptamer-based multiplexed proteomics approach to identify novel serum

213 in changes identified with the aptamer-based multiplexed proteomics approach used in this study can b

214 Quantitative multiplexed proteomics was used to provide for the first

215 re, we present a highly sensitive, rapid and multiplexed protocol termed UbiFast for quantifying ~10,

216 To address this gap, we used multiplexed proximity biotinylation (BioID) to generate

217 ula-based FFD method can potentially allow a multiplexed "QCR-on-chip" technology, bringing a paradig

218 This multiplex qPCR assay could support early clinical diagno

219 ence-based singleplex and non-matrix scoring multiplex qPCR assays utilize a hydrolysis probe, provid

220 A) for discovery proteomics, but traditional multiplexed quantification approaches employing mass dif

221 Here, we describe a strategy that permits multiplexed quantification by DIA using mass defect-base

222 (DSP) allows for spatially resolved, highly multiplexed quantification of proteins in clinical sampl

223 ion at specific loci, with the potential for multiplexed quantifications, expanding the current toolk

224 In this paper, multiplex quantitative polymerase chain reaction (qPCR)

225 Isobaric tags enable multiplexed quantitative analysis of many biological sam

226 ation of 11,266 unique phosphopeptides using multiplexed quantitative mass spectrometry.

227 rbate peroxidase(5) in mouse hearts, and use multiplexed quantitative proteomics(6,7) to track hundre

228 based on peptide-coupled reporter ions is a multiplexed quantitative strategy in proteomics that all

229 o phase-stable PICs marks a key step towards multiplexed quantum repeaters(7,8) and general-purpose q

230 We performed quantitative multiplex real-time polymerase chain reaction (PCR) for

231 Multiplex real-time polymerase chain reactions were perf

232 We designed a multiplex real-time quantitative PCR (qPCR) assay to det

233 rpin probes in an alternating DNA copolymer, multiplex recognition of different miRNAs was demonstrat

234 Here, we propose a novel method for multiplexing, referred to as amplification and melting c

235 a range of DOC was tested using an in vitro multiplex reporter system that evaluates modulation of t

236 sed in research environments with high-level multiplexing, representing a major challenge.

237 Here, we have coupled multiplexed repression of neurodevelopmental disease-ass

238 he volume of required reagents, we devised a multiplex RT-qPCR assay to detect SARS-CoV-2 in a single

239 Our novel multiplex RT-qPCR improves upon current single diagnosti

240 The cycle threshold (Ct) values of our multiplex RT-qPCR were comparable to those obtained by t

241 -CoV-2 RNA were consistently detected by the multiplex RT-qPCR.

242 We developed a multiplex SARS-CoV-2 antibody immunoassay based on Lumin

243 dancy and maximise discovery, we undertook a multiplex screen of 80 apoptotic-inducing agents in paed

244 Multiplexed screening against barcoded substrates yielde

245 w 96-well plate methodology for fast, enzyme-multiplexed screening for metabolite-protein adducts was

246 ap collision voltages or by implementing mAb-multiplexed SEC-CIU analyses to maximize information con

247 Samples were tested by multiplex semi-quantitative PCR for 12 viruses.

248 opment and clinical testing of a disposable, multiplexed sensing device (ToMMx), which is a portable,

249 This multiplexed sensing system enabled the real-time monitor

250 n-weighted breast images.Purpose: To compare multiplexed sensitivity-encoding (MUSE) diffusion-weight

251 Multishot multiplexed sensitivity-encoding diffusion-weighted imag

252 s challenging to simultaneously achieve high multiplexing, sensitivity, and throughput in single-cell

253 the repeat unit in this VNTR, along with our multiplexed sequencing strategy, provides an unprecedent

254 igenic epitopes and develop high performance multiplex serological assays.

255 Multiplexed seroprofiles for EBV, malaria, and KSHV were

256 strates the specificity and potential of our multiplexed SERS imaging platform in the study of fatty

257 Multiplex single-molecule fluorescent in situ hybridizat

258 g AAV9 (adeno-associated virus 9) to deliver multiplexed single guide RNAs against Ube2v1 in cardiac-

259 is a recently introduced method to quantify multiplexed single-cell proteomes.

260 Here we used multiplexed single-cell RNA-seq to profile 198 cancer ce

261 address these gaps, we utilized quantitative multiplexed single-molecule fluorescence in situ hybridi

262 DNA gap repair in E coli supports efficient multiplex site-directed mutagenesis.

263 bination with targeted mass spectrometry for multiplexed site-specific PTM quantitation of monoclonal

264 Described here is a rapid multiplex SMA dPCR genotyping assay run on a fully integ

265 We used a combination of multiplex SNaPshot assays, Sanger sequencing, mutation-s

266 Multiplex spatial analyses dissect the heterogeneous cel

267 atial Profiling (DSP) is a method for highly multiplex spatial profiling of proteins or RNAs suitable

268 -free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological

269 and isobaric tagging (cPILOT) is an enhanced multiplexing strategy currently capable of analyzing up

270 instrument and utilizes both an existing ion multiplexing strategy to achieve up to an 8-fold gain in

271 trate a spectrum of rigid-yet-flexible super-multiplex structures for encoded fluorescence detection

272 t optical assessment and thus will allow for multiplexing, such that reactions could be conducted, si

273 ral applicability of DNA-PAINT-ERS for fast, multiplexed superresolution imaging using previously val

274 ptide tandem mass tag (TMT) labeling, sample multiplexing, synchronous precursor selection (SPS), and

275 We show that our multiplexed synthesis method can be used to store digita

276 Using a multiplex system, we assayed plasma biomarkers related t

277 ermore, we demonstrated, for the first time, multiplexed target detection in microwells, both from bu

278 We used multiplexed, targeted mass spectrometry (MS) to quantify

279 We demonstrate an optical frequency-domain multiplexing technique, allowing high-fidelity initializ

280 ore, application and integration of advanced multiplex techniques has been extremely limited.

281 Multiplex tests allow for measurement of allergen-specif

282 Multiplex tests for respiratory viruses that are repeate

283 phase transition of the hydrogels, realizing multiplexed thermal image- and distance-based visual qua

284 half of the recorded VTA DA neurons perform multiplexing: they exploit the phasic and tonic activity

285 d that overcomes major impediments to highly multiplex tissue imaging.

286 Using multiplexed tissue imaging, we observed HSF1 foci in hum

287 reserving on-target editing, and they can be multiplexed to suppress several off-targets simultaneous

288 Here, we develop MIX-Seq, a method for multiplexed transcriptional profiling of post-perturbati

289 Using the multiplexed tri-mode signal outputs, the PT-Disk can qua

290 This approach makes customizable multiplex ultrasensitive ELISA available to laboratories

291 tum holography technology that is capable of multiplexing up to 200 independent orbital angular momen

292 Moreover, we demonstrate the potential for multiplexing using a digital high-resolution melt (dHRM)

293 plex with the potential for higher levels of multiplexing using commercially available (13)C/(15)N la

294 MAP), to probe metabolic fluxes by utilizing multiplexed vibrational metabolic probes.

295 the same physical fiber (Wavelength Division Multiplexing, WDM).

296 per are theoretical, to motivate the idea of multiplexing, we provide experimental evidence that tent

297 information from data to enable data-driven multiplexing with high accuracy.

298 provides an affordable method of high-level multiplexing without fluorescent probes, extending the b

299 e of issues with diagnostic tests, including multiplexing, workflow simplicity, and reduced turnaroun

300 can yield large cost savings especially when multiplexed workflows are considered.