ライフサイエンスコーパス: multiplex PCR

1 the repertoires generated by 5'-RACE PCR and multiplex PCR.

2 as investigated in 43 isolates of amoebae by multiplex PCR.

3 species identification using a 16S rRNA gene multiplex PCR.

4 omposition of sequence libraries prepared by multiplex PCR.

5 esence of viral infection was ascertained by multiplex PCR.

6 tested to perform high-throughput nanoliter multiplex PCR.

7 -well design of the SlipChip were tested for multiplex PCR.

8 at used broth enrichment prior to sequential multiplex PCR.

9 almonella DNA microarray, and prophage-based multiplex PCR.

10 high-resolution subtyping not possible with multiplex PCR.

11 -NGS is applicable to all areas that rely on multiplex PCR.

12 interference that is common to conventional multiplex PCR.

13 eptibility, API 20S assay, and genotyping by multiplex PCR.

14 e dismutase (sodA) gene for development of a multiplex PCR.

15 that far exceed the capacity of traditional multiplex PCR.

16 -linking, chromatin immunoprecipitation, and multiplex PCR.

17 PC), bla(NDM), and bla(OXA-48)-type genes by multiplex PCR.

18 PCR amplification, which may be enhanced by multiplex PCR.

19 logic path toward maximizing the benefit of multiplex PCR.

20 aminoglycoside-modifying enzyme genes using multiplex PCR.

21 sitive, and amenable to both single-step and multiplex PCRs.

22 pathogenic fungi can be identified with two multiplex PCRs.

23 ultiple sexually transmitted infections, and multiplex PCRs.

24 Eight multiplex PCRs, 32 targeting serotype-determining capsul

25 To our diagnostic S. aureus multiplex PCR, a mecC primer set was designed and implem

26 good agreement between our single reaction, multiplexed PCR/AFM data, and data from 20 individual si

27 nalyzed by single-cell reverse transcriptase multiplex PCR after patch clamp and was compared with th

28 The targets were generated by multiplex PCR, allowing simultaneous amplifications of 4

29 next-generation sequencing, termed anchored multiplex PCR (AMP), that is compatible with low nucleic

30 Here, we carried out a combination of multiplex PCR, amplicon quantification, and next generat

31 edical Products), which uses target-enriched multiplex PCR amplification followed by liquid array ide

32 pproach used in this study involves one-tube multiplex PCR amplification of six target bacterial viru

33 Fluorescent output of multiplexed PCR amplification could also be imaged with

34 iquid samples and analyzes the samples using multiplexed PCR amplification coupled with microsphere a

35 The multiplexed PCR amplified the multiply repeated IS481 B.

36 d positive and negative predictive values of multiplex PCR analyses were 87, 96, 94, and 93%, respect

37 A combination of oligonucleotide arrays, multiplex PCR analysis and single-nucleotide polymorphis

38 Firstly, multiplex PCR analysis indicated that the novel strain b

39 By combining patch-clamp and RT-multiplex PCR analysis of individual neurons in mouse br

40 Multiplex PCR analysis revealed the types of mutants to

41 All of these strains were serotyped, and multiplex PCR analysis was used to ascertain the presenc

42 Nested multiplex PCR analysis was used to type four independent

43 s were identified to the species level using multiplex PCR and 16S rRNA gene sequencing.

44 We present a method based on multiplex PCR and array detection of amplicons to assay

45 ymorphic markers in the same reaction with a multiplex PCR and base extension reaction.

46 V strains of each species type and tested by multiplex PCR and culture.

47 cing method that couples microfluidics-based multiplex PCR and deep sequencing (mmPCR-seq) to uniform

48 Using multiplex PCR and DNA sequencing, we identified a single

49 and Escherichia coli based on triple-tagging multiplex PCR and electrochemical magneto genosensing on

50 otide polymorphisms in 19 candidate genes by multiplex PCR and hybridization to an immobilized probe

51 mpacted subsequent HPV detection in both the multiplex PCR and Linear Array assays.

52 x PCR and standard Linear Array to 0.881 for multiplex PCR and modified Linear Array.

53 e then examined all patients by microfluidic multiplex PCR and next-generation sequencing for all 27

54 HPV status agreements ranged from 0.615 for multiplex PCR and standard Linear Array to 0.881 for mul

55 ossible sequence variations, using extensive multiplex PCR and two-dimensional electrophoretic separa

56 even serotype 6C isolates were identified by multiplex PCR and/or Quellung reaction.

57 cost and effort up to 50-fold, by combining multiplex PCRs and DNA extractions from pools of presort

58 signature design from genome-wide datasets, multiplex-PCR and detection by single-base extension and

59 By multiplexing PCR and pooling multiplexed reactions toget

60 de-modifying enzymes (AMEs) were detected by multiplex PCR, and clonal relationships were determined

61 accessory gene regulator group genotyping by multiplex PCR, and identification of toxin and potential

62 Newer technologies, such as multiplex PCR, and novel diagnostic platforms that incor

63 The broth enrichment, conventional multiplex PCR, and real-time PCR approaches used in this

64 were screened for mcr-1 to mcr-5 genes by a multiplex PCR, and their genetic diversity was measured

65 A rapid multiplex PCR approach was developed to detect the bft g

66 E)-polymerase chain reaction (PCR) and three multiplex-PCR approaches, when applied to the same T cel

67 polymerase chain reaction [PCR]), including multiplex PCR, as well as and microscopy for protozoal i

68 ortrait Staph ID/R blood culture panel (BCP) multiplex PCR assay (Great Basin Scientific, Salt Lake C

69 sion of the FilmArray Respiratory Panel (RP) multiplex PCR assay (Idaho Technology, Inc., Salt Lake C

70 This high-throughput multiplex PCR assay allowed simple and accurate typing o

71 We then used this information to design a multiplex PCR assay based on the simultaneous amplificat

72 MuPlex is uniquely designed for large-scale multiplex PCR assay design in an automated high-throughp

73 cal specimens from Bangladesh were used, the multiplex PCR assay detected 95% (21 of 22) of Giardia m

74 mbination of murG and mur-2(ed) primers in a multiplex PCR assay differentiated E. hirae from E. dura

75 s were used to construct a semiquantitative, multiplex PCR assay for BV based on detection of 3 posit

76 A rapid real-time multiplex PCR assay for detecting and differentiating Bo

77 A multiplex PCR assay for GBS capsular gene typing was als

78 A multiplex PCR assay for identification of each of these

79 developed and extensively validated a novel multiplex PCR assay for ST1193 and its characteristic fi

80 atory virus detection in the upper airway by multiplex PCR assay is common in critically ill hematolo

81 Multiplex PCR assay may prove helpful for the risk strat

82 n with primers to detect PVL, we developed a multiplex PCR assay specific for the identification of U

83 dge, this is the first report of a real-time multiplex PCR assay that can be used for the simultaneou

84 rep-B-Latex agglutination method and a novel multiplex PCR assay that detected all 10 GBS capsular ty

85 terial genomic data, we developed a one-step multiplex PCR assay to identify Salmonella and simultane

86 For this purpose, we have designed a single multiplex PCR assay to simultaneously amplify 95 diagnos

87 A real-time, multiplex PCR assay using HRM was designed for the detec

88 Direct multiplex PCR assay using vanA and vanB primers, which p

89 A real-time multiplex PCR assay was developed to allow detection of

90 The multiplex PCR assay was found to be a specific and sensi

91 The multiplex PCR assay was specific at discriminating each

92 Furthermore, a multiplex PCR assay was validated for rapid molecular di

93 Genetically tagged UPEC and a multiplex PCR assay were employed to investigate the dis

94 nd, we have combined a fluorogenic real-time multiplex PCR assay with four sets of degenerate PCR pri

95 is study, we compared a laboratory-developed multiplex PCR assay with primers and probes specific for

96 Finally, a novel multiplex PCR assay, based on random amplified polymorph

97 that 97% were correctly identified using the multiplex PCR assay.

98 frozen at ICU admission were tested using a multiplex PCR assay.

99 olates were all classified as type A using a multiplex PCR assay.

100 ved Shigella invasion antigen, IpaH3, into a multiplex PCR assay.

101 cterial, 3 viral, and 3 parasite) commercial multiplex PCR assay.

102 First, a semiquantitative, multiplexed PCR assay (BV-PCR) and the Affirm VPIII Gard

103 Second, a multiplexed PCR assay detecting Candida albicans and Can

104 agnostics LLC, Salt Lake City, UT), a highly multiplexed PCR assay, can identify 24 etiologic agents

105 replacing conventional stool testing with a multiplexed PCR assay, without an increase in testing co

106 MuPlex designs a set of multiplex PCR assays designed to cover as many of the in

107 Qiagen Spin blood kit held as the constant, multiplex PCR assays detect more HPV-positive specimens

108 However, the design of a multiplex PCR assays is computationally challenging beca

109 Previous multiplex PCR assays took hours to days from order time

110 Subsequently, simple multiplex PCR assays were designed on the basis of these

111 New multiplex PCR assays were developed for mating-type dete

112 ime human papillomavirus (HPV) type-specific multiplex PCR assays were developed to detect HPV DNA in

113 Multiplex PCR assays were developed to identify Actinoba

114 These multiplex PCR assays, based on prophage-like elements an

115 d HPV59 in type- and gene-specific real-time multiplex PCR assays.

116 uPlex that aids researchers in the design of multiplex PCR assays.

117 , Wageningen, the Netherlands) and published multiplex PCR assays.

118 Multiplexed PCR assays increase the detection of diarrhe

119 When analyzed by multiplex PCR at the single-cell level, normal splenic P

120 which enabled the retrieval of duplex UMI in multiplex PCR based enrichment and sequencing.

121 We have developed a strategy for multiplex PCR based on PCR suppression.

122 Here, we describe the development of a multiplex PCR, based on variation within the capsule loc

123 Here, we designed and validated a novel multiplex PCR-based assay for STc648 that took advantage

124 Thus, this novel multiplex PCR-based assay should enable any laboratory e

125 To address this topic, we devised a multiplex PCR-based assay, termed TAR (telomere-associat

126 nts with blood culture pathogens on a rapid, multiplex PCR-based blood culture identification panel (

127 The multiplex PCR-based NGS is useful and robust to acquire

128 A novel multiplex PCR-based NGS was developed to gather whole ge

129 We report here a multicenter validation of a multiplex-PCR-based strategy previously developed by Q.

130 Using a highly multiplexed PCR-based target enrichment method (RainDanc

131 Simple, multiplexed, PCR-based barcoding of DNA for sensitive mu

132 The multiplex PCR can distinguish 17 individual serotypes in

133 The RMA utilizes improved multiplex PCR chemistry (EraGen MultiCode-PLx technology

134 The NanoChip400 system uses multiplex PCR chemistry and electronic microarray detect

135 y viruses uses an expanded genetic alphabet, multiplex PCR chemistry, and microsphere flow cytometry

136 Sputum multiplex PCR could become a useful diagnostic tool for

137 fication artifacts typically associated with multiplex PCR derived from the use of many primer pairs

138 The multiplex PCR described therefore represents a simple an

139 trol that was undetectable by very sensitive multiplex PCR, despite increasing antibodies.

140 The multiplex PCR detected organisms in 26 of the 30 true-po

141 Multiplex PCR detected potentially significant bacteria

142 leic acid amplification system that provides multiplexed PCR detection of the influenza A, influenza

143 ampC multiplex PCR differentiated the six plasmid-mediated am

144 rising an online primer design tool, a novel multiplex PCR enrichment protocol, optimized library pre

145 efore, we developed the Pneumoplex assays, a multiplex PCR-enzyme hybridization assay (the standard a

146 Therefore, we developed a multiplex PCR-enzyme hybridization assay, the Adenoplex,

147 MPD can successfully design multiplex PCR experiments suitable for next-generation s

148 rce software that can design next-generation multiplex PCR experiments that ensures primers are uniqu

149 Multiplex PCR experiments were performed on isolates to

150 A rapid "mega"-multiplex PCR (FilmArray gastrointestinal panel; BioFire

151 ventional methods in the first season and by multiplex PCR (FilmArray) in the second season.

152 ix to eight diagnostic sequences/organism by multiplex PCR; fluorescent labeling of the amplicons via

153 ygosity mapping, whole-exome sequencing, and multiplex PCR followed by next-generation sequencing.

154 We then developed multiplex PCR for amplification of these 15 loci in four

155 either random nucleic acid amplification or multiplex PCR for GAS detection.

156 Multiplex PCR for genus and species determination of ent

157 Fingerprinting by multiplex PCR for IS900 integration loci clustered 67 of

158 tuberculosis isolates representing different multiplex PCR for IS900 loci (MPIL) or amplified fragmen

159 sting, pulsed-field gel electrophoresis, and multiplex PCR for phylotyping to determine their resista

160 tic signatures, along with a newly developed multiplex PCR for rapid differentiation between "wild-ty

161 Our method relies on multiplex PCR for targeted enrichment of viral genomes f

162 We developed a real-time fluorescence-based multiplex PCR for the detection of all six of the curren

163 Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to

164 We have developed a multiplex PCR for the detection of these Mycoplasmatales

165 These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic re

166 This report describes the development of a multiplex PCR for the purpose of identifying family-spec

167 udy proposes an authentication protocol with multiplex PCR for three species of snappers (Lutjanus pu

168 tory symptoms and LRT and URT RV testing via multiplex PCR from 2009 to 2016 were included.

169 rology and nasopharyngeal swab specimens for multiplex PCR from case patients and controls.

170 nd obtained oropharyngeal swab specimens for multiplex PCR from case patients, and serum for serology

171 Consequently, SmaI-multiplex PCR has the potential to be used in routine cl

172 Multiplex PCR has the potential to rapidly identify bloo

173 Real-time multiplex PCR has the potential to serve as an adjunct t

174 Multiplex PCR identified T. nativa from the bear meat, a

175 One multiplex PCR identifies serogroup D, A, and B and Vi-po

176 59 months, we compared organism detection by multiplex PCR in IS and nasopharyngeal/oropharyngeal (NP

177 Amplification is initiated by a multiplex PCR in this case with 170 primer pairs.

178 and schematic sequence-based system of seven multiplex PCRs, in a sequence order based upon Active Ba

179 dvantages of using the SlipChip platform for multiplex PCR include the ability to preload arrays of d

180 We perform multiplex PCR inside a capillary, transfer the amplified

181 Multiplex PCR is a key technology for an endless list of

182 Melting-point analysis of real-time multiplex PCR is a rapid, sensitive, specific, and inexp

183 Multiplex PCR is defined as the simultaneous amplificati

184 Thus, a multiplex PCR is designed by which known mutations in C4

185 Despite these difficulties, multiplex PCR is frequently used in applications such as

186 The multiplex PCR-LDR assay goes beyond other PCR-based assa

187 Application of the multiplex PCR-LDR assay will provide the sensitivity and

188 We have developed a multiplex PCR-ligase detection reaction (LDR) assay that

189 A multiplex PCR-ligation detection reaction (PCR-LDR) assa

190 A multiplex-PCR Luminex xMAP bead probe fluid array using

191 This multiplex PCR-Luminex assay enables sensitive, specific,

192 Using a multiplex PCR, major changes were detected in 'on/off' s

193 with carbapenemase-producing organisms using multiplex PCR may help hospitals to improve infection co

194 d Xpert MTB/RIF, and nasopharyngeal aspirate multiplex PCR.Measurements and Main Results: In 459 pati

195 A multiplex PCR method coupled with allele-specific oligon

196 A multiplex PCR method for determination of capsule types

197 A multiplex PCR method has been developed to differentiate

198 h, high error rate, and undetermined bias of multiplex PCR method have hindered broader application o

199 This multiplex PCR method provides a rapid, simple, and relia

200 ers targeting prp and cps were combined in a multiplex PCR method that defines species using the tlh

201 This study used a nested multiplex PCR method to detect three periodontal pathoge

202 A multiplex PCR method was developed to identify simultane

203 We have tested a multiplex PCR method which shows the presence or absence

204 Resistance genes were confirmed by a multiplex PCR method with the vanC gene detected in all

205 This report describes a multiplex PCR method, the Mycobacterial IDentification a

206 ains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and

207 r, only 1 of these 12 was Vi negative by the multiplex PCR method.

208 Here, we present a multiplexed PCR method for targeted sequencing of select

209 Isolates were characterized by using multiplex PCR methodology to determine structural types

210 ed to identify 8 serovars (9 genovars) in 12 multiplex PCR mixes on 11 S. enterica strains.

211 ing microbiologic, serologic, and sequential multiplex PCR (MP-PCR) techniques to serotype the isolat

212 A six gene multiplex PCR (mPCR) assay, designed to effectively diff

213 The multiplex PCR (mPCR) distinguished between all previousl

214 A multiplex PCR (mPCR) was constructed based on these gene

215 MIA results were validated by multiplex PCR (mPCR).

216 Capsular serotypes were determined by multiplex PCR, multibead assay, or latex agglutination.

217 ating DNA methylation of multiple loci via a multiplex PCR (multiplexed-scAEBS).

218 identification immunoassays, and orthogonal, multiplexed PCR (nucleic acid) amplification and detecti

219 nd serotype 6C was differentiated from 6A by multiplex PCR of DNA extracts by using appropriate prime

220 Multiplex PCR of IS900 integration loci (MPIL) and ampli

221 of oligonucleotides to amplicons produced by multiplexed PCR of genomic DNA, individual microspheres

222 Multiplex PCR offers a rapid, sensitive, and easy method

223 We developed two multiplex PCRs, one end-point and one real-time with mel

224 Primer approximation multiplex PCR (PAMP) is a new experimental protocol for

225 The first FDA-approved multiplex PCR panel for a large number of respiratory pa

226 A multiplex PCR panel targeting these five genes was used

227 We performed a clinical evaluation of a multiplex PCR panel, the enteric parasite panel (EPP), f

228 We evaluated a multiplexed PCR panel for the detection of 16 bacterial,

229 l phosphotriester modifications in improving multiplex PCR performance.

230 nt a novel and exciting avenue for improving multiplex PCR performance.

231 . falciparum and P. vivax in conventional or multiplex PCR platforms.

232 hm, and demonstrate its utility in compiling Multiplex PCR primer panels for detection and differenti

233 Twenty multiplex PCR/primer extension reactions were set up and

234 oftware package that automates the design of multiplex PCR primers for next-generation sequencing.

235 Eighteen sets of virus-specific multiplex PCR primers were developed based on the conser

236 ion PCR method involving the purification of multiplex PCR products followed by uniplex analysis on a

237 Multiplex PCR products were electrochemically detected i

238 nique, termed deligotyping, which hybridizes multiplex-PCR products to membrane-bound, highly specifi

239 We report the development of a multiplex PCR protocol for the diagnosis of staphylococc

240 A two-round multiplex PCR protocol was used to amplify these sequenc

241 were determined using several well-validated multiplex PCR protocols culled from the literature and s

242 g themselves but differed from the RNA-based multiplex-PCR results.

243 es were serotyped by latex agglutination and multiplex PCR-reverse line blotting (mPCR/RLB).

244 al evaluating outcomes associated with rapid multiplex PCR (rmPCR) detection of bacteria, fungi, and

245 The assay consisted of a multiplexed PCR set of 5 tubes able to detect the most c

246 Multiplex PCR shows that the region including the G-quad

247 ssessment of the SmaI restriction site-based multiplex PCR (SmaI-multiplex PCR) typing (SMT) with res

248 The implementation of multiplex PCR stool testing was associated with a reduct

249 data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherich

250 ecifically assigned 1 of 17 serotypes by the multiplex PCR system, with the results in complete conco

251 rogroup 6 isolates can be identified using a multiplex PCR system; however, due to the high sequence

252 d using an innovative strategy consisting of multiplexing PCR, targeted sequencing and computational

253 dentify CA-MRSA strains are based on complex multiplex PCRs targeting the staphylococcal cassette chr

254 e aggregative adherence fimbriae (AAFs) by a multiplex PCR, targeting the four known major subunit va

255 In conclusion, a multiplex PCR technique was developed for identifying fa

256 Using real time and multiplex PCR techniques, we identified three germline h

257 y have allowed for development of single and multiplexed PCR techniques that provide rapid detection

258 mpared to a clinically validated traditional multiplexed PCR test with additional sequence analysis a

259 ated the impact of the introduction of rapid multiplex PCR testing using the FilmArray gastrointestin

260 Multiplex PCR testing was also utilized for Ambler class

261 The results of a multiplex PCR that could detect 25 bacterial and fungal

262 By using a multiplex PCR that specifically amplifies several genes,

263 of human-pathogenic species, we developed a multiplex PCR that uses species-specific primers to ampl

264 m infection with six organisms identified by multiplex PCR that was initially thought to be a monomic

265 Nested Patch PCR, a novel method for highly multiplexed PCR that is very specific, can sensitively d

266 By multiplex PCR, the dominantly expressed form of human B7

267 As determined by a multiplex PCR, the E. coli ONT:H25 isolates carried a co

268 Using multiplex PCR, the prevalence of these 12 and 3 addition

269 Through the use of multiplexed PCR, the two mutation types were amplified i

270 A multiplex PCR to differentiate S. hyicus, S. agnetis, an

271 Three sets of primers were used in one multiplex PCR to identify three different species.

272 Genomic DNA was amplified by multiplex PCR to produce amplicons of the three p53 exon

273 ntly involve tyrosine kinases, we designed a multiplex PCR to search for mRNA fusions between BCR and

274 Subsequently, we designed a multiplex PCR to target three genetic markers that diffe

275 ssay, a novel diagnostic tool which utilizes multiplex PCR to test for 21 respiratory pathogens with

276 I restriction site-based multiplex PCR (SmaI-multiplex PCR) typing (SMT) with respect to pulsed-field

277 reated patients using VNTR combined with 16S multiplex PCR unambiguously and reliably distinguished d

278 mavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers.

279 An improved multiplex PCR, using redesigned primers targeting the se

280 This multiplex PCR was compared to culture on 26 cervical swa

281 Accuracy of identification of the multiplex PCR was determined by comparisons to standard

282 The SmaI-multiplex PCR was found to be more discriminatory than M

283 ntibiotic susceptibility was determined, and multiplex PCR was performed for OXA-23-like, -24-like, -

284 Multiplex PCR was performed on oral rinses collected fro

285 The multiplex PCR was tested on isolated DNA or fungal colon

286 An assay of multiplex PCR was used for the typing.

287 Multiplex PCR was used to compare the E. coli strains fo

288 Multiplex PCR was validated on the 384-well SlipChip wit

289 The limits of the multiplex PCR were 2.8 x 10(-2) CFU/microl PCR mixture f

290 Here, we report the development of a robust multiplex PCR which has assisted in the detection of 32

291 nical isolates of Acinetobacter species in a multiplex PCR, which also detects bla(OXA-23-like) and c

292 regions of each of these targets in a single multiplex PCR while incorporating a 6-carboxyfluorescein

293 Here we use a multiplex PCR with a mixture of primers targeting the re

294 nerally requires either latex agglutination, multiplex PCR with analysis of band sizes, or analysis o

295 iotic use may be realized by combining rapid multiplex PCR with provider education, clinical decision

296 s was developed that can unite the reward of multiplex PCR with real-time PCR to recognize animal gen

297 This is the first method that couples multiplex PCR with sample-specific DNA barcodes and next

298 TB-DzT combines a multiplex PCR with single nucleotide polymorphism (SNP)

299 methylation-sensitive restriction enzyme and multiplexed PCR with gene-specific primers (MSRE-PCR).

300 cific to different target genes are used for multiplex-PCR, with one primer for each target being lab