ライフサイエンスコーパス: mutagenesis study

1 cluding key residues predicted in a previous mutagenesis study.

2 approach was employed, followed by a focused mutagenesis study.

3 in GLAST has been delineated in an elaborate mutagenesis study.

4 tivator, thorough kinetic investigation, and mutagenesis studies.

5 f the proteins and a number of site-directed mutagenesis studies.

6 ture is consistent with previously published mutagenesis studies.

7 s first conducted, followed by site-directed mutagenesis studies.

8 dicted ARB affinity, which aligns with early mutagenesis studies.

9 findings and those of previous experimental mutagenesis studies.

10 ystallography and by conducting solution and mutagenesis studies.

11 combined molecular docking and site directed mutagenesis studies.

12 demonstrating excellent agreement with past mutagenesis studies.

13 cal for binding to GIP through site-directed mutagenesis studies.

14 mination have come from transposon insertion mutagenesis studies.

15 ur predicted structure agrees with available mutagenesis studies.

16 to the assembly domain defined previously by mutagenesis studies.

17 center of the trimer, which was confirmed by mutagenesis studies.

18 TGATN4ATCAA-3' in these target sequences via mutagenesis studies.

19 f substrate specificity were complemented by mutagenesis studies.

20 cleotides in the 5' stem-loop is revealed by mutagenesis studies.

21 esult in different findings in whole-protein mutagenesis studies.

22 l linkage analysis, electron microscopy, and mutagenesis studies.

23 A site-directed mutagenesis study allowed the identification of three di

24 Single- and double-point amino acid mutagenesis studies along with whole-cell electrophysiol

25 Mutagenesis studies also revealed that NQO1Y127/Y129 req

26 s to phase separation experimentally through mutagenesis studies and computationally through direct i

27 tructure and similar sequences, we performed mutagenesis studies and determined the key role of the V

28 This was supported by mutagenesis studies and experiments with alpha-conotoxin

29 Mutagenesis studies and fluorescence spectroscopy valida

30 Further structural analysis using mutagenesis studies and molecular docking revealed the m

31 or, S-adenosyl-l-homocysteine, together with mutagenesis studies and molecular docking simulations, u

32 Previous mutagenesis studies and photoaffinity labeling using lig

33 This property enabled a transposon mutagenesis study and growth studies to confirm novel ge

34 This mechanism, further supported by mutagenesis study and the structure of monofunctional gl

35 Substrates docking is validated by mutagenesis studies, and a structure-based catalytic mec

36 romoter sequence analyses, deletion studies, mutagenesis studies, and electrophoretic mobility shift

37 alorimetry (ITC), stopped-flow measurements, mutagenesis studies, and molecular dynamics (MD) simulat

38 in complex with a DNA substrate, followed by mutagenesis studies, and propose a common mechanism by w

39 sing secondary kinetic isotope effect (KIE), mutagenesis study, and primary KIEs.

40 ionship (SAR), crystallography, docking, and mutagenesis studies are used to examine the binding mode

41 bility and can serve as a platform for novel mutagenesis studies as well as a point for comparison wi

42 We combined systematic mutagenesis studies at the luteinizing hormone receptor

43 nique mechanism is provided by site-directed mutagenesis studies, biophysical characterization of the

44 With regard to the CR2-binding site on C3d, mutagenesis studies by Isenman and coworkers have implic

45 Mutagenesis studies confirm and functionally define the

46 Mutagenesis studies confirm that hydrophobic residues in

47 Our mutagenesis studies confirm that the CPSF30-hFip1 comple

48 Mutagenesis studies confirm that the interface observed

49 Mutagenesis studies confirm that the ion pairs at the in

50 Mutagenesis studies confirm the contribution of the hydr

51 Biochemical and mutagenesis studies confirm the stoichiometry and other

52 Mutagenesis studies confirmed a critical role for apoA1

53 Mutagenesis studies confirmed that Glu-16 is critical fo

54 Mutagenesis studies confirmed that inhibition of EBOV vi

55 Mutagenesis studies confirmed that polymorphisms at amin

56 Mutagenesis studies confirmed that Spider Roll binds the

57 The HS-binding site, identified by mutagenesis study, consists of eight basic residues that

58 mapping to the surface of the structure and mutagenesis studies demarcated a hotspot likely to inter

59 Mutagenesis studies demonstrate that entry into this pro

60 Mutagenesis studies demonstrate that the [2Fe-2S] cluste

61 Furthermore, mutagenesis studies demonstrate that the glycosylation p

62 cks sodium channels from the open state, and mutagenesis studies demonstrate that this particle uses

63 Mutagenesis studies demonstrate the critical role of con

64 Finally, mutagenesis studies demonstrated residues in the back po

65 Furthermore, proteolysis and site-directed mutagenesis studies demonstrated that 1G08 Fab binds a r

66 Mutagenesis studies demonstrated that a functional AF-2

67 Deletion mutagenesis studies demonstrated that expression of a sh

68 Mutagenesis studies demonstrated that KIAA1549-BRAF fusi

69 Structure-based site-directed mutagenesis studies demonstrated that R(91), at the tip

70 Mutagenesis studies demonstrated that Ser(47) within his

71 Mutagenesis studies demonstrated that the S17 insert was

72 Structure guided mutagenesis studies demonstrated that three salt bridges

73 Mutagenesis studies designed to probe for base-pairing i

74 In this mutagenesis study, each of the five base-specific amino

75 Crystallographic and mutagenesis studies elucidated a key binding interaction

76 Bioinformatic analysis and mutagenesis studies elucidated the biosynthetic pathway

77 mpounds, assessment of water energetics, and mutagenesis studies enables SAR exploration to map GPCR-

78 Structure-guided mutagenesis studies establish a crucial role for this su

79 Sequence analysis and mutagenesis studies establish that this effect stems fro

80 trained based on a dataset of comprehensive mutagenesis studies for 151 PPI complexes, with experime

81 Mutagenesis studies further confirmed that the interacti

82 Time-lapse imaging and mutagenesis studies further establish a positive correla

83 Mutagenesis studies further established that Cys-199 is

84 Mutagenesis studies further map the functional interface

85 Published modeling and site-directed mutagenesis studies had previously shown that the residu

86 Previous mutagenesis studies had shown that MYOD1 with a p.Leu122

87 ture of a P2X4 receptor, in combination with mutagenesis studies, has provided a model of the intersu

88 Although previous mutagenesis studies have demonstrated the importance of

89 Sequence analysis and mutagenesis studies have elucidated a nucleotide sequenc

90 based protein footprinting and site-directed mutagenesis studies have enabled us to map several inter

91 complex, but available crystal structures or mutagenesis studies have failed to identify such residue

92 Recent mutagenesis studies have identified a mutant G4C/S10C/T1

93 tiple sites, including Trp(72) Site-specific mutagenesis studies have suggested, but have not conclus

94 Mutagenesis studies identified a single residue (positio

95 Mutagenesis studies identified a YYXXF sorting signal in

96 n, competitive inhibition, and site-directed mutagenesis studies identified exosite 2 as the site of

97 Mutagenesis studies identified interaction of the PIAS S

98 In silico docking and mutagenesis studies identified the (E)-2-dodecenal bindi

99 In addition, these mutagenesis studies identified three mutants, R786A, R82

100 A previous random mutagenesis study identified a W165Y/E166Y/P167G triple

101 A recent mutagenesis study identified transmembrane region (TM)4

102 Mutagenesis studies identify a critical G3-loop located

103 Mutagenesis studies identify critical residues in the TM

104 Mutagenesis studies identify LspA as an aspartyl peptida

105 Substrate trapping and mutagenesis studies identify PKM2 Tyr-105 and Tyr-148 as

106 Biochemical and mutagenesis studies illustrate how CAR-mediated clusteri

107 Mutagenesis studies implicate Lys265 as the oxygen activ

108 Mutagenesis studies implicated the transmembrane regions

109 Prior site-directed mutagenesis studies in bacterial ketosteroid isomerase (

110 co homology modelling, molecular docking and mutagenesis studies in combination with substrate bindin

111 Mutagenesis studies in conjunction with structure-activi

112 biotin biosynthesis genes during transposon mutagenesis studies in mouse infection models(6-9).

113 This was confirmed by a site-directed mutagenesis study in MDCK cells.

114 rtant binding sites were further analyzed by mutagenesis studies, in which corresponding vMIP-II muta

115 Molecular modelling and mutagenesis studies indicate that agonist positive allos

116 Site-directed mutagenesis studies indicate that amino acid residues pr

117 Mutagenesis studies indicate that the compounds are orth

118 Mutagenesis studies indicate that the small molecules ac

119 Mutagenesis studies indicate that these fibrils are depe

120 Mutagenesis studies indicated FAK-His58 is critical for

121 Mutagenesis studies indicated that binding of Glu and Ca

122 In this study, complementation and mutagenesis studies indicated that representatives of al

123 o prediction of water network energetics and mutagenesis studies indicated that the displacement of a

124 Mutagenesis studies indicated that the reactivity and sp

125 Site-directed mutagenesis studies indicated that the transcription fac

126 Furthermore, cross-linking and mutagenesis studies indicated that this second binding s

127 Mutagenesis studies indicated that VRC01 contacts within

128 A meta-analysis of mutagenesis studies indicated these predicted loops are

129 ing these existing approaches in large-scale mutagenesis studies is limited by the technical challeng

130 This interaction was confirmed by mutagenesis studies, making 7-phenyl-2-aminoquinolines t

131 ometry, and sequence-specific antibodies and mutagenesis studies now unambiguously establish phosphor

132 Site-directed mutagenesis studies of a strictly conserved T201 residue

133 pe are monomeric proteins, and site-directed mutagenesis studies of AtsB reveal that individual Cys -

134 Structural and mutagenesis studies of Eis indicate that its acetylation

135 Biophysical, genome mapping, and mutagenesis studies of FoxA1 reveals two different modes

136 of the HCT/HQT, we performed structural and mutagenesis studies of HCT.

137 utility of Rhapsody by in silico saturation mutagenesis studies of human H-Ras, phosphatase and tens

138 Site-directed mutagenesis studies of K65R and T69del assessed the phen

139 is function have been determined by in vitro mutagenesis studies of laboratory-adapted HIV-1 molecula

140 Based on our combined computational and mutagenesis studies of MhsT and LeuT, we propose that TM

141 While previous mutagenesis studies of PYDs point to the involvement of

142 Mutagenesis studies of the enzyme TbtD identified import

143 Mutagenesis studies of the galabiose-binding domain of t

144 Deletion and mutagenesis studies of the hrg-1 promoter revealed a 23-

145 ulation of Na(+),K(+)-ATPase was explored in mutagenesis studies of the potential PKA site at Ser-938

146 Moreover, mutagenesis studies of the receptor revealed key positio

147 immunoprecipitation (ChIP) and site-directed mutagenesis studies of the RON promoter, we identified N

148 Mutagenesis studies of the UL11 N terminus revealed that

149 Mutagenesis studies of these sequences showed that bulky

150 We further performed mutagenesis studies of this loop to probe its role in su

151 Extensive mutagenesis studies of three distinct TRBV11-2(+) TCRs f

152 Further, a mutagenesis study of CgAcr3-1 suggested that a conserved

153 Mutagenesis study of PIP5K1A reveals two adjacent interf

154 In an elaborate mutagenesis study of the 5-HT(3)A receptor guided by a h

155 313, and A316, were identified in a scanning mutagenesis study of the BK (Ca(2+)-activated, large-con

156 Mutagenesis study of this conserved domain in Golgin45 s

157 Thus, whole-protein mutagenesis studies offer an acceptable means of identif

158 Systematic mutagenesis studies on 100 mutants of fascin indicate th

159 is consistent with the limited site-directed mutagenesis studies on 2B6 and extensive studies on P450

160 s (SeSaM followed by epPCR), site saturation mutagenesis studies on individual positions, and one sim

161 Furthermore, our mutagenesis studies on NES-H12 suggest that altered shut

162 We conducted mutagenesis studies on several conserved residues that a

163 Mutagenesis studies on specific residues abolish the iso

164 oxin family members, combined with extensive mutagenesis studies on SubB, show how the hydrophobic pa

165 We report structural, computational, and mutagenesis studies on the catalytic and resistance mech

166 Mutagenesis studies on the core Aib residue involved in

167 In particular, deep mutagenesis studies on the distribution of fitness effec

168 Mutagenesis studies on the IA and ECL were guided by our

169 Site-directed mutagenesis studies on this enzyme and its substrate had

170 y basic residues, we performed site-directed mutagenesis studies on this enzyme, revealing that two a

171 Mutagenesis studies on three functionally impaired EC Ne

172 In addition, mutagenesis studies on VyPAL1-3, VyAEP1, and VcAEP suppo

173 Combined with mutagenesis study, our structural analyses elucidate two

174 and, in conjunction with the biochemical and mutagenesis studies presented here, delineate the underl

175 t it accurately correlates with experimental mutagenesis studies probing the mutational change in mea

176 NiR with a donor protein, AxNiR-cytc551, and mutagenesis studies provide direct evidence for the impo

177 Computational and mutagenesis studies provide strong support for a dominan

178 Our mutagenesis studies provide the first experimental suppo

179 Mutagenesis studies reveal a delicate balance between ch

180 Our bioinformatic analysis and mutagenesis studies reveal that heterotetrameric ChsE1-C

181 Pulse-chases combined with mutagenesis studies reveal that Ser1 strongly influences

182 gen/deuterium exchange mass spectrometry and mutagenesis studies reveal that the engagement of the di

183 ecular dynamics simulations, biochemical and mutagenesis studies reveal that the palmitoylation inser

184 Mutagenesis studies reveal that the region D1 in p27 pla

185 Mutagenesis studies reveal that two extracellular residu

186 Structural analysis and complementary mutagenesis studies reveal the basis for recognition and

187 ral analysis together with computational and mutagenesis studies reveal the molecular mechanisms of t

188 ated a 580-bp human miR143/145 enhancer, and mutagenesis studies revealed a critical role for both a

189 Site-directed mutagenesis studies revealed four substitutions in OsMTP

190 Mutagenesis studies revealed that Asp(202) and Glu(361)

191 Detailed mutagenesis studies revealed that each Sp site made a po

192 Further enzymatic and mutagenesis studies revealed that GarA acted as a dynami

193 Further mutagenesis studies revealed that HIV-1 Env, and the V3

194 Site-directed mutagenesis studies revealed that imperatorin most likel

195 Conditional mutagenesis studies revealed that MeCP2 dysfunction in e

196 Truncation and alanine mutagenesis studies revealed that PP2Ac binds to the P3

197 Mutagenesis studies revealed that SSR42 codes for an 891

198 PLSases, our functional characterization and mutagenesis studies revealed that Subdued is a bona fide

199 rometry analysis combined with site-directed mutagenesis studies revealed that the lysine couple Lys(

200 Mutagenesis studies revealed that the pilin-dependent mo

201 Although initial mutagenesis studies revealed that the signature DEAD-box

202 Mutagenesis studies revealed that these effects required

203 Subsequent mutagenesis studies revealed that two residues in the ac

204 Structure-activity relationship, docking and mutagenesis studies revealed the crucial interactions fo

205 Mutagenesis studies revealed the crucial role of the C-t

206 Structural and mutagenesis studies revealed the molecular underpinnings

207 saminyl 6-phosphomethylmannoside, along with mutagenesis studies, revealed the residues involved in d

208 Structural, kinetics, and mutagenesis studies show that CB7 binds to the aldehyde

209 X-ray crystallography and mutagenesis studies show that mannose is ligated to the

210 Electrophysiology and mutagenesis studies show that prokaryotic TRICs have sim

211 Site-directed mutagenesis studies show that single-point mutations wit

212 ucture of the ATPaseCW cassette of MORC4 and mutagenesis studies show that the DNA-binding site and t

213 ctroscopy measurements and the site-directed mutagenesis studies show that the red Fur protein binds

214 Mutagenesis studies showed that an extra subdomain of ab

215 Mutagenesis studies showed that both hydrophobic and ele

216 Mutagenesis studies showed that covalent modification of

217 Site-directed mutagenesis studies showed that Cys-805, Cys-930, and Cy

218 Mutagenesis studies showed that LFNG modification of O-f

219 Site-directed mutagenesis studies showed that the core promoter and PA

220 Mutagenesis studies showed that the major recognition de

221 Mutagenesis studies showed that three positively charged

222 Mutagenesis studies showed that tyrosine-774 is critical

223 Site-directed mutagenesis studies showed that Y361 and H412 were criti

224 Our A3G mutagenesis study showed that lysine residues 297, 301,

225 Mutagenesis study showed that the ability of MCPIP1 to r

226 Homology modeling and mutagenesis study showed that the locations of the carbo

227 two inhibitors are provided on the basis of mutagenesis studies, structure-activity relationship ana

228 Our mutagenesis study subsequently identified VP1 C11 and C1

229 eta3 subunits as templates for site-directed mutagenesis studies, substitution with mbeta3 subunit re

230 Site-directed mutagenesis studies suggest that at least predicted alph

231 Mutagenesis studies suggest that calmodulin binding to t

232 However, mutagenesis studies suggest that Ile-A10, Ser-A12, Leu-A

233 Mutagenesis studies suggest that minor changes in the en

234 Mass spectrometry and mutagenesis studies suggest that phosphorylation of both

235 C1), a structurally related ion channel, and mutagenesis studies suggest that the large extracellular

236 Mutagenesis studies suggest that the observed transcript

237 Molecular modeling and site-directed mutagenesis studies suggest that the stimulatory compoun

238 However, mutagenesis studies suggest that the tetrametric form of

239 Molecular modeling and site-directed mutagenesis studies suggest that two residues in helix 7

240 Furthermore, mutagenesis studies suggest that Val-499 is the primary

241 Mutagenesis studies suggest the electrostatic repulsion

242 Mutagenesis studies suggest the involvement of invariant

243 ons were determined and, in combination with mutagenesis studies, suggest that both Srr1 and Srr2 int

244 These data, along with mutagenesis studies, suggest that debranching (not inhib

245 Recent mutagenesis studies suggested a potential activation sit

246 Mutagenesis studies suggested that both RING and B-box m

247 Further mutagenesis studies suggested that mutation at position

248 Further mutagenesis studies suggested this linker also plays an

249 steps in virion assembly, this comprehensive mutagenesis study suggests yet another role for NS2 in l

250 This analysis, along with mutagenesis studies, suggests that the inhibitor binds a

251 Extensive site-directed mutagenesis studies supported the importance of this phe

252 fic adenosine 2'-O-methylation, rationalizes mutagenesis studies targeting the K61-D146-K180-E216 enz

253 We have demonstrated in previous mutagenesis studies that accumulation of the covalent co

254 relationship was confirmed by comprehensive mutagenesis studies that also reveal the importance of i

255 ular dynamics simulations, and corroborative mutagenesis studies, the appropriate binding pockets for

256 A in its open ring form, in conjunction with mutagenesis studies, the potential substrate binding and

257 Through mutagenesis studies, the putative disulfide bond pairs i

258 Together with mutagenesis studies, these data suggest a model for subs

259 In line with mutagenesis studies, these gating modalities resulted fr

260 Together with docking and mutagenesis studies, these structures provide a comprehe

261 d by molecular dynamics (MD) simulations and mutagenesis studies, these structures reveal conformatio

262 eport structural, biochemical and cell-based mutagenesis studies to further characterize A3A's deamin

263 tly, this structure provides new targets for mutagenesis studies to further understand and define thi

264 Previous mutagenesis studies to identify the binding site on PAI-

265 2 and support the development of large-scale mutagenesis studies to identify viral variants with uniq

266 ly in silico molecular modeling with in vivo mutagenesis studies to investigate TCR-pMHC interactions

267 ritical residues that were shown in previous mutagenesis studies to mediate GPR124 binding and WNT7A/

268 ms, we consider herein what the outcome of a mutagenesis study truly reveals about an allosteric mech

269 logical phosphomimetics, supporting numerous mutagenesis studies using such approaches, and illustrat

270 Both forward and reverse mutagenesis studies validate the impact of one or a few

271 ico hA(3)AR-homology model and site-directed mutagenesis study was performed to demonstrate that amin

272 n the basis of these models, a site-directed mutagenesis study was subsequently conducted that focuse

273 Using structural and site-directed mutagenesis studies, we demonstrate that ECG binds to th

274 Through mutagenesis studies, we demonstrate that phosphorylation

275 study, using computational and site-directed mutagenesis studies, we demonstrate the presence of stro

276 By truncation and mutagenesis studies, we demonstrated that the second alp

277 ained at 2.4- angstrom resolution coupled to mutagenesis studies, we discovered ligase-activity deter

278 Together with mutagenesis studies, we elucidate the conformational tra

279 Through mutagenesis studies, we find that the ability of Org 275

280 omoter-luciferase system in combination with mutagenesis studies, we found that the polymorphic allel

281 By comparison with tyrosine recombinases and mutagenesis studies, we have been able to define some of

282 tructural and sequence alignments as well as mutagenesis studies, we have identified the key residues

283 Through deletion and site-directed mutagenesis studies, we have identified Thr-680 as the m

284 y, homology modeling, molecular docking, and mutagenesis studies, we have located the substrate-bindi

285 Through mutagenesis studies, we identified crucial residues requ

286 ough bioinformatics, molecular modeling, and mutagenesis studies, we identified the putative NSC75609

287 trometry, combined with in vitro and in vivo mutagenesis studies, we identified the regions involved

288 Using truncation and mutagenesis studies, we mapped the auto-dephosphorylatio

289 Employing mutagenesis studies, we show that DDD could operate inde

290 Through mutagenesis studies, we show that phosphosites within th

291 Based on a comprehensive site-directed mutagenesis study, we localized the polySia attachment s

292 ified with the two models, and site-directed mutagenesis studies were conducted to validate the model

293 Site-directed mutagenesis studies were consistent with bisubstrate bin

294 oscopy, molecular modeling, and double-cycle mutagenesis studies were integrated to obtain a structur

295 modeling, species ortholog comparisons, and mutagenesis studies were then employed to define the mol

296 Receptor chimera and site-directed mutagenesis studies were used to validate these binding

297 In addition, these mutagenesis studies will aid in HDAC1-inhibitor design t

298 h EC1 and C15a were probed and identified by mutagenesis studies with four different RV-C genotypes.

299 environment in spectral tuning by combining mutagenesis studies with spectroscopic (UV-vis) and theo

300 Detailed mutagenesis studies within this region revealed that fou