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1 ggests that they are relatively common among mycobacteriophages.
2 eric hosts and the phage L5-related group of mycobacteriophages.
4 ation of PA6 resembled that of the temperate mycobacteriophages, although the genome was much smaller
5 cribe what we have learned from the study of mycobacteriophages and how a holistic approach-integrati
8 isters can be visualized using dual-reporter mycobacteriophages and their formation prevented using r
9 ntifiable recombination proteins are rare in mycobacteriophages, and only 1 of 30 genomically charact
12 hat have plagued their genetic manipulation, mycobacteriophages are especially appealing subjects for
15 e have developed a luciferase-based reporter mycobacteriophage assay that can determine drug resistan
16 on vector can be easily adapted to different mycobacteriophage attachment sites (attB) due to its mod
21 nstrates a high degree of similarity to many mycobacteriophages both morphologically and genetically.
24 ore than 60% of the genes unrelated to other mycobacteriophages, but offers novel insights into how m
28 ite-specific integration was mediated by the mycobacteriophage Bxb1 integrase-catalyzed recombination
32 cribe a pair of serine integrases encoded by mycobacteriophages Bxz2 and Peaches with unusual and unp
33 and Cjw1, which are distinguished from other mycobacteriophages by their possession of a Pnkp enzyme,
37 , and only 1 of 30 genomically characterized mycobacteriophages (Che9c) encodes homologs of both RecE
40 cobacteria illustrate this model and a large mycobacteriophage collection reveals the enormous divers
42 ese challenges by utilizing real-time PCR of mycobacteriophage D29 DNA to evaluate the drug resistanc
48 onally replicating shuttle phasmids from the mycobacteriophages D29 and TM4 that enable efficient del
49 of the phams have sequence similarity to non-mycobacteriophage database entries, and fewer than 10% o
52 e system by identification and expression of mycobacteriophage-encoded recombination proteins, adapti
55 aracterized the virulence of three different mycobacteriophages (Fionnbharth, Muddy, and D29), alone
56 atic investigation of the virulence of three mycobacteriophages, Fionnbharth, Muddy, and D29, and the
58 193 unrelated genes encoded by 13 different mycobacteriophages for their ability to impair the growt
60 e limitations-together with the abundance of mycobacteriophage genes of unknown function-could be add
62 hermosensitive mutations were created in the mycobacteriophage genome that allow replication at 30 de
63 However, the relatively high G+C% content of mycobacteriophage genomes (64.1%) can be challenging for
64 A set of 2,600 fully sequenced and annotated mycobacteriophage genomes and the development of tools f
67 se chemistry, the reconstruction of complete mycobacteriophage genomes using High-Complexity Golden G
68 eplete with novel genes not present in other mycobacteriophage genomes, and although most are of unkn
69 literature estimates, extrapolating from 14 mycobacteriophage genomes, suggesting that two billion p
71 describe a functional genomic dissection of mycobacteriophage Giles, in which the virion proteins ar
73 tic determination of the genome sequences of mycobacteriophages has revealed the presence of several
74 70 complete genome sequences available, the mycobacteriophages have provided a wealth of information
77 we provide the X-ray crystal structure of a mycobacteriophage immunity repressor bound to DNA, which
78 of a Pnkp enzyme, are also unique among the mycobacteriophages in their specification of putative RN
79 f features not previously described in other mycobacteriophages, including noncanonical genome archit
81 to visualize the spatiotemporal dynamics of mycobacteriophage infection in single cells and populati
83 These results inform us on the dynamics of mycobacteriophage infections, both alone and in cocktail
85 ycobacterial lysis, a Giles DeltalysB mutant mycobacteriophage is viable, but defective in the normal
88 ning the phage attachment site attP from the mycobacteriophage L5 genome and additionally containing
97 that shares a similar genome organization to mycobacteriophage L5, although the two phages are hetero
98 that it is a close relative of the temperate mycobacteriophage L5, and is presumably a non-temperate
99 that it is a close relative of the temperate mycobacteriophage L5, whose sequence has been described
102 logy of Bxb1 particles is similar to that of mycobacteriophages L5 and D29, although Bxb1 differs fro
105 We developed a novel luciferase reporter mycobacteriophage (LRM) based phenotypic DST method usin
107 e previously described a luciferase reporter mycobacteriophage (LRP) assay that can detect Mycobacter
109 ains encoding up to three kill switches: two mycobacteriophage lysin operons negatively regulated by
115 P protocol is the ability of the recombinant mycobacteriophage phAE40 to infect a variety of Mycobact
119 hermore, rarefaction analysis shows that the mycobacteriophage population is not closed, and there is
121 cobacteriophages, which-together with the 83 mycobacteriophages previously reported-represent the lar
124 , and new methods for simple construction of mycobacteriophage recombinants will facilitate postgenom
126 Characterization of ten Cluster N temperate mycobacteriophages revealed at least five distinct proph
129 The genomic sequences of ten newly isolated mycobacteriophages suggest that the bacteriophage popula
132 on of foreign DNA using the broad-host range mycobacteriophage TM4 and the application of the lucifer
142 ere the complete genome sequences of 138 new mycobacteriophages, which-together with the 83 mycobacte