ライフサイエンスコーパス: myotome

1 ment (newly formed somites, dermomyotome and myotome).

2 ls have migrated toward the periphery of the myotome.

3 sociated with cytodifferentiation within the myotome.

4 cone is still navigating towards the ventral myotome.

5 nscripts become restricted to the developing myotome.

6 uld act through beta-catenin on cells in the myotome.

7 dorsal (dMNs) and ventral (vMNs) parts of a myotome.

8 esoderm as it differentiates into the mature myotome.

9 e for deficiencies of the lateral (hypaxial) myotome.

10 ed the different origins of myoblasts in the myotome.

11 n the DRG, and myogenesis in the neighboring myotome.

12 reduced or disturbed myoD expression in the myotome.

13 s tissue, and induces formation of a lateral myotome.

14 e survival and differentiation of the dorsal myotome.

15 branches per axon length within the ventral myotome.

16 myotome, but is not expressed in the forming myotome.

17 that innervate cell-specific regions of the myotome.

18 d to impair their migration into the ventral myotome.

19 nnervate cell-specific regions of each trunk myotome.

20 alising factors act in concert to induce the myotome.

21 axon along the medial surface of the ventral myotome.

22 tiation of basement membrane assembly in the myotome.

23 yotomes rather than predominantly to any one myotome.

24 in adult rodent muscle and in the developing myotome.

25 somite and is subsequently excluded from the myotome.

26 er particularly in the epaxial region of the myotome.

27 l further growth of the epaxial and hypaxial myotome.

28 al layer of slow-twitch muscle of the mature myotome.

29 ith NLRR-1 being expressed in the developing myotome.

30 eral sclerotome, adjacent to and beneath the myotome.

31 nervating distinct regions of the developing myotome.

32 al for motor axon outgrowth into the ventral myotome.

33 actors and the FGF signal originating in the myotome.

34 diolateral expansion of the dermomyotome and myotome.

35 in vertebrate embryos appear in the somitic myotome.

36 low-twitch muscle cell migration through the myotome.

37 in response to FGF signaling in the adjacent myotome.

38 he ventrolateral and dorsomedial lips of the myotome.

39 rtilization, powered by muscles of the axial myotomes.

40 vibrissal and hair follicles, limb buds, and myotomes.

41 rowth directions of the epaxial and hypaxial myotomes.

42 he epaxial myotomes, but not in the hypaxial myotomes.

43 is required for Fgf4-lacZ expression in the myotomes.

44 oderm and later become incorporated into the myotomes.

45 ic vesicles, the roof of midbrain, and trunk myotomes.

46 x, necessary to drive gene expression in the myotomes.

47 motoneurons, MiPs, extend axons along dorsal myotomes.

48 embryogenesis, large muscle segments, called myotomes, acquire a characteristic chevron morphology, w

49 ne expression was not detected in the dorsal myotome and a high level of apoptosis was observed with

50 undergoing differentiation, specifically the myotome and a subpopulation of differentiating myoblasts

51 lay in motor axon extension into the ventral myotome and aberrant branching of these motor axons.

52 anslated region necessary and sufficient for myotome and AER expression.

53 from the En1-positive dermomyotome enter the myotome and dermatome, thereby superimposing the En1-Sim

54 ional growth and segmental patterning of the myotome and dermomyotome bear interesting similarities w

55 driving the growth and morphogenesis of the myotome and dermomyotome in the epaxial domain of the bo

56 along the dorsoventral axis into sclerotome, myotome and dermomyotome.

57 ove to the external surface of the embryonic myotome and express the transcription factor Pax7.

58 at Hes6 is expressed in the murine embryonic myotome and is induced on C2C12 myoblast differentiation

59 In contrast, in the lateral myotome and migratory somitic cells, which require the e

60 We also demonstrated prominent expression in myotome and muscle fibres in human foetuses, and zebrafi

61 RNA transcripts were highly expressed in the myotome and premuscle mass.

62 r models for the developmental regulation of myotome and sclerotome formation in somites through dist

63 Myotome and sclerotome precursor cells are derived, resp

64 x1 and QmyoD differ greatly, suggesting that myotome and sclerotome specification are controlled by d

65 lopment including cartilage differentiation, myotome and sclerotome specification, hair follicle deve

66 e emerge from two distinct compartments, the myotome and sclerotome, in response to signals secreted

67 phases that are governed principally by the myotome and sclerotome, respectively.

68 on markers for the somitogenic tissue types, myotome and sclerotome, respectively.

69 the growth and morphogenesis of the primary myotome and simultaneously drive that of the dermomyotom

70 progenitors of two of the axial tissues, the myotome and spinal cord.

71 wth and morphogenesis of the epaxial primary myotome and the overlying dermomyotome epithelium.

72 Reciprocal defects in signaling between the myotome and the sclerotome compartments of the somites i

73 The mechanical coupling between the future myotome and the surrounding tissues appears to spatially

74 rs in the more ventral-lateral region of the myotome and the youngest fibers in the dorsomedial regio

75 chain are normally expressed in the ventral myotome and there is normal development of hypaxial musc

76 Lateral presomitic cells remain deep in the myotome and they differentiate into fast muscle fibers.

77 n restricting the CaP pathway to the ventral myotome and thus to neuromuscular specificity.

78 ation into dorsal dermomyotome, intermediate myotome and ventral sclerotome.

79 ement responsible for Fgf4 expression in the myotomes and AER, and showed that a conserved E-box is a

80 cally reduced expression of Fgf4 mRNA in the myotomes and AER.

81 erized, muscle fibers elongate into adjacent myotomes and are abnormally long.

82 ession of Xenopus cardiac alpha-actin in the myotomes and developing heart tube of the tadpole requir

83 -2/-133a-1 intragenic enhancer in the somite myotomes and in all skeletal muscle fibers during embryo

84 nhancer element directing Fgf4 expression in myotomes and limb bud AER, and that its activity in the

85 l differentiation or the interaction between myotomes and surrounding tissues during morphogenesis.

86 n postimplantation expression in the somitic myotomes and the limb bud AER.

87 rate from the dermomyotome and move into the myotome, and later in myotomal precursors destined to mi

88 ession of molecular markers for dermomytome, myotome, and sclerotome, indicating that Shh might not b

89 near the dorsal and ventral extremes of the myotome, and this muscle growth continues into larval li

90 ricted regions of the neural tube, in caudal myotomes, and at the materno-embryonic junction of the u

91 es, including the ICM of the blastocyst, the myotomes, and the limb bud AER, is regulated by distinct

92 g the inner cell mass of the blastocyst, the myotomes, and the limb bud apical ectodermal ridge (AER)

93 l surface and some incorporate into the fast myotome, apparently by moving between differentiated slo

94 The precursors to the embryonic epaxial myotome are concentrated in the dorsomedial lip (DML) of

95 Transients produced within the myotome are correlated with somitogenesis as well as myo

96 myoblasts derived from the medial (epaxial) myotome are not present to compensate for deficiencies o

97 Fish myotomes are comprised of anatomically segregated fast a

98 gular and somite borders do not form; later, myotomes are fused.

99 ggest that initially both dorsal and ventral myotomes are permissive for CaP axons but as development

100 precursor cells for the epaxial and hypaxial myotomes are predominantly located in the dorsomedial an

101 spinal cord, the notochord and the segmented myotomes - are found in the regenerated tail.

102 in topped mutants fail to enter the ventral myotome at the proper time, stalling at the nascent hori

103 tion in transgene expression specifically in myotomes at 11.5 days.

104 ction of the Notch ligand, DeltaD); however, myotome boundaries form later ("recover") in a Hedgehog-

105 esize that migrating slow muscle facilitates myotome boundary formation in aei/deltaD mutant embryos

106 The third stage is myotome boundary formation, where the boundary becomes r

107 s suggests that slow muscle is necessary for myotome boundary recovery in the absence of initial epit

108 sed in the skeletal muscle precursors of the myotome, branchial arches and limbs as well as in the de

109 n in the differentiating muscle of embryonic myotomes but not in newly formed somites prior to muscle

110 nopus embryos results in an expansion of the myotome, but suppression of terminal muscle differentiat

111 e expression of Myf5 and MyoD in the epaxial myotomes, but not in the hypaxial myotomes.

112 IP(3) and ryanodine receptors in the intact myotome by eliciting Ca(2+) elevations in response to ph

113 We measured AChR aggregation in myotomes by labeling them with rhodamine-alpha-bungaroto

114 e in development, including the neural tube, myotome, cartilage, and sites of epithelial-mesenchymal

115 hick-quail marking experiments show that new myotome cells in such recombinant somites are derived fr

116 here to analyze the location and pattern of myotome cells whose precursors had earlier been labeled

117 ting that NLRR-1 is expressed in a subset of myotome cells.

118 yos revealed notochord involvement in dorsal myotome change of permissiveness.

119 itch muscle cells migrate through the entire myotome, coming to lie at its most lateral surface.

120 nesis, GDF-8 expression is restricted to the myotome compartment of developing somites.

121 ression was developmentally regulated in the myotome compartment of mouse somites and that its down-r

122 segmentation and formation of the somite and myotome compartment.

123 N-cadherin/catenins remain expressed by the myotome compartment.

124 rison of Myf-5 mRNA levels in the psm and in myotome-containing somites indicates about a 10-fold dif

125 No myotome defects are observed in Gnai3/Gnai1 double-mutan

126 n/MRF4-mutant combinations displayed ventral myotome defects, including a failure to express normal l

127 (AChRs) formed in the central region of the myotome, destined to be synapse-rich, before axons exten

128 n terminally differentiates, suggesting that myotome development requires additional signals.

129 s or pre-somitic mesoderm (segmental plate), myotome development was evident but was delayed or other

130 d MRF4 have overlapping functions in ventral myotome differentiation and intercostal muscle morphogen

131 becomes independent of axial signals during myotome differentiation when somites activate expression

132 tely following somite formation and prior to myotome differentiation.

133 is site demonstrate that cells fated to form myotome do not involute around the recurved epithelium o

134 found that zebrafish sema5A was expressed in myotomes during the period of motor axon outgrowth.

135 s that a conserved E box located in the Fgf4 myotome enhancer is required for Fgf4-lacZ expression in

136 ry factors, which appear to be essential for myotome expression.

137 d sequestered to the coelomic linings during myotome extension.

138 Notably, both the dermomyotome and myotome fail to adopt a normal epithelial morphology in

139 We found that the mutant dermomyotomes and myotomes failed to organize and to elongate medially and

140 Finally, deposition of myotome fibers and expansion of the dermomyotome epithel

141 Analysis of nascent myotome fibers showed that they elongate along the embry

142 ons of the dermomyotome did not give rise to myotome fibers.

143 pendent decrease in permissiveness of dorsal myotome for CaP axonal outgrowth.

144 ssive, showing that permissiveness of dorsal myotome for normal CaP pathfinding diminished over time.

145 ssary within the somite for dermomyotome and myotome formation and delamination of limb myogenic prog

146 This new model for early myotome formation has implications for myogenic specific

147 Myotome formation in the epaxial and hypaxial domains of

148 Therefore, models of epaxial myotome formation must account for the positioning of th

149 lies of myogenic regulators and may regulate myotome formation temporally or spatially.

150 in the dermomyotome, is necessary for early myotome formation, while the subsequent phases are assoc

151 d, which appear to stem from a deficiency in myotome formation.

152 ally specify the dermamyotome, and later the myotome from it, have been controversial.

153 Dorsal and ventral myotomes from both younger and older floating head mutan

154 Dorsal myotomes from young embryos were also permissive for CaP

155 DBHR is also expressed in the myotome, from the earliest stages of its formation, and

156 Myotome growth and morphogenesis can be restored in a DM

157 t different stages of development shows that myotome growth begins earlier in the epaxial than in the

158 Subsequent myotome growth occurs by the addition of new muscle fibe

159 he dermomyotome epithelium overlying the new myotome growth region and from which the mesenchymal der

160 rmomyotome epithelium blocks further primary myotome growth while ablation of other dermomyotome regi

161 yogenic precursors responsible for zebrafish myotome growth.

162 derm that differentiates medially within the myotome, immediately adjacent to the notochord.

163 identify an essential role for the specified myotome in axial tendon development, and suggest that ab

164 as sclerotome in some vertebrate species and myotome in others.

165 ene was used to examine the formation of the myotome in the paraxis-/- background.

166 t-twitch myocyte fusion within the zebrafish myotome in toto.

167 ole in the expansion of the dermomyotome and myotome in Wnt-3a-treated embryos.

168 c embryos showed specific LacZ expression in myotomes in a pattern that closely resembles the express

169 nsible for activating Fgf4 expression in the myotomes in a spatial- and temporal-specific manner.

170 Native myotomes were replaced with donor myotomes in normal or reversed dorsoventral orientations

171 D can sustain Fgf4 expression in the ventral myotomes in the absence of Myf5.

172 stained application of glutamate to skeletal myotomes in vivo is necessary and sufficient for up-regu

173 growth factor (FGF) signal secreted from the myotome induces formation of a scleraxis (Scx)-expressin

174 med somitic compartments, the sclerotome and myotome, interact to establish this fourth Scx-positive

175 o give rise to sclerotome, dermomyotome, and myotome involves the coordination of many different cell

176 As the vertebrate myotome is generated, myogenic precursor cells undergo e

177 e dermomyotome, translocate to the subjacent myotome layer to form this first segmented muscle tissue

178 momyotome epithelium and enter the subjacent myotome layer where myogenic differentiation ensues.

179 ural tube, trunk skeletal muscle precursors (myotomes), limb skeletal anlagen, craniofacial regions,

180 yonic stages of myogenesis in the developing myotome, limb bud precursors, and heart tube, but by lat

181 l spinal cord in accordance with established myotome maps of the upper extremity.

182 orrelates with a deficit in dermomyotome and myotome marker gene expression, suggesting that Ripply1

183 end on signals from the embryo, since dorsal myotomes matured in vitro remained permissive for CaP ax

184 sed muscle contractile proteins confirms the myotome mediolateral growth directions, and shows that t

185 that muscle progenitor cells populating the myotome migrate aberrantly in the ventral somite in the

186 Dye-labeled myotome myofibers were generated from cells injected alo

187 n the Neo1 locus (encoding neogenin) develop myotomes normally but have small myofibers at embryonic

188 cell types in the neural tube, pancreas and myotome of con mutant and Disp1 morphant embryos, we con

189 2 transcription factors are expressed in the myotome of developing somites and cooperatively activate

190 e isolated NLRR-1 as a gene expressed in the myotome of developing somites but not in the presomitic

191 FA ligand expression was not detected in the myotome of Myf5 null mutant embryos and PDGFA promoter a

192 est skeletal muscle to organize, the primary myotome of the epaxial domain, is a thin sheet of muscle

193 is expressed in the developing dermatome and myotome of the somite, epidermis, gut endoderm, the epit

194 entricular layer of the neural tube, and the myotome of the somites.

195 d jamc2, were not detected in the somites or myotome of wild-type embryos.

196 15, cXin expression is also detected in the myotomes of developing somites.

197 Hom3a region to drive lacZ expression in the myotomes of transgenic mice.

198 ating expression of these genes in the early myotomes of wildtype embryos.

199 lips of the dermomyotome and entry into the myotome or dispersal into the periphery.

200 cell fates that promote either dermomyotome/myotome or sclerotome differentiation, respectively.

201 However, expression of Fgf4 in the myotomes or AER of murine embryos does not appear to be

202 ndrial mass, which correlated with defective myotome organization during zebrafish muscle development

203 the expression of either protein within the myotome perturbs migration.

204 ndent labeling experiments demonstrated that myotome precursor cells translocate directly from the mi

205 Myotome precursor cells undergo myotomal translocation;

206 h the hypothesis that successive lineages of myotome precursor cells with different mitotic and morph

207 Myotome precursor cells, by contrast, appear to be deter

208 To precisely locate myotome precursor cells, fluorescent vital dyes were ion

209 ory models have been proposed to explain how myotome precursor cells, which are known to reside withi

210 Gax was detected in myotomes, premuscle masses, and mature muscle groups.

211 single muscle cells to nearly all segmental myotomes rather than predominantly to any one myotome.

212 motor axons from branching into surrounding myotome regions.

213 tereotyped projections to ventral and dorsal myotome regions.

214 d limb bud AER, and that its activity in the myotomes results at least in part from the synergistic a

215 ecessary for the complete patterning of each myotome segment.

216 Myotome shape can be altered by perturbing muscle cell d

217 heavy chain transcripts, as well as overall myotome size and individual fiber size, but had no effec

218 flanking sequence, each can interact with a myotome-specific distal enhancer of cardiac alpha-actin

219 The myotome-specific elements contain binding sites for bHLH

220 Pax1 and QmyoD are early sclerotome and myotome-specific genes that are activated in epithelial

221 conclusion that patterning and growth in the myotome specifically, and in the epaxial and hypaxial do

222 tion of pax1 and QmyoD during sclerotome and myotome specification, and suggest specific molecular mo

223 ion from the presomitic mesoderm, the future myotome spreads across the underlying tissues.

224 ite fusion being highly stochastic, a robust myotome structure emerges at the tissue scale.

225 l-caudal boundary setting, specialization of myotome subdivisions or the specific RAR subtype that is

226 tes at the medial and lateral aspects of the myotome, suggesting the existence of at least one other

227 patterns is a region over the middle of the myotomes that is relatively free of melanophores.

228 (Xic1) is highly expressed in the developing myotome, that ablation of p27(Xic1) protein prevents mus

229 In addition to the myotome, the transplanted DML also gives rise to the der

230 gulated in embryonic development by E10.5 in myotomes, the progenitors of skeletal muscle, supporting

231 dissociated neural tubes and their adjacent myotomes, the resultant protein disrupted both desmin fi

232 ateral growth directions, and shows that the myotome thickness increases in a superficial (near dermi

233 sts that Shh mediates Fgf4 activation in the myotomes through mechanisms independent of its role in t

234 the CaP pathway remained present on ventral myotome throughout development.

235 yf5 is subsequently expressed in the epaxial myotome under the control of other elements located far

236 Slow-twitch muscle fibers of the zebrafish myotome undergo a unique set of morphogenetic cell movem

237 ion, the identity of the responding tissues, myotome versus sclerotome, differs.

238 s are generated at the ventral extent of the myotome via bifurcation of the growth cone.

239 PDGFA expression in the myotome was fully restored in embryos in which MyoD has

240 nisms contributing to shape formation of the myotome, we combine single-cell resolution live imaging

241 an assay to ask whether other regions of the myotome were permissive for normal CaP pathfinding.

242 missive for CaP axons, however, older dorsal myotomes were non-permissive, showing that permissivenes

243 Ventral myotomes were permissive for CaP axons, even when they w

244 Native myotomes were replaced with donor myotomes in normal or

245 ontal stripe over the lateral surface of the myotomes where otherwise abundant, neural crest-derived

246 at myotendinous junctions in somites and the myotome, where it co-localizes with beta1-integrin, vinc

247 In the medial myotome, where the expression of the myogenic factor Myf

248 ve axonal path to the lateral surface of the myotome, where they develop into slow-twitching muscle f

249 mbranes, but was concentrated at the ends of myotomes, where AChRs normally aggregate at synapses.

250 ves are also delayed in entering the ventral myotome whereas all other axons examined, including dors

251 h of developing motor axons into the ventral myotome whereas the sema domain mediates repulsion and k

252 vy chain first appear ventrolaterally in the myotome, whereas slow myosin heavy chain transcripts fir

253 , CaPs, pioneer axon outgrowth along ventral myotomes; whereas, middle primary motoneurons, MiPs, ext

254 adherin and M-cadherin expression within the myotome, which correlate precisely with cell migration,

255 only in the epaxial but also in the hypaxial myotomes, while it is maintained in the AER.

256 Myf5 is required for Fgf4 expression in the myotomes, while MyoD is not, but MyoD can sustain Fgf4 e

257 morphologically continuous dermomyotome and myotome, whose epaxial-hypaxial subdivision and hence th

258 d to controls and E(2)+AI treatment restored myotome width and height accompanied by some dramatic ch

259 transients occur in cells of the developing myotome with characteristics remarkably similar to those

260 Development of the myotome within somites depends on unknown signals from t

261 lower motor neuron degeneration in multiple myotomes, without sensory neuropathy.