ライフサイエンスコーパス: nanodisc

1 lf-assembled native protein-lipid complexes (Nanodiscs).

2 g interface of the P450-cytb5 complex in the nanodisc.

3 ane-bound protein YopB inserted into a lipid nanodisc.

4 represents the structure and dynamics of the nanodisc.

5 ations by linear dichroism measurements in a Nanodisc.

6 thK and in channels reconstituted into lipid nanodiscs.

7 were formed in the presence of bicelles and nanodiscs.

8 ture of the mouse TRPML1 channel embedded in nanodiscs.

9 PC1 that was incorporated into lipid bilayer nanodiscs.

10 ystems such as membrane proteins embedded in nanodiscs.

11 yloid polypeptide intermediate stabilized in nanodiscs.

12 nts after its cotranslational insertion into nanodiscs.

13 tificial lipid bilayers such as liposomes or nanodiscs.

14 and reconstitution of membrane proteins into nanodiscs.

15 is via functional reconstitution of Aer into nanodiscs.

16 r homodimers inserted into lipid bilayers of Nanodiscs.

17 n vitro self-assembling phospholipid bilayer nanodiscs.

18 d streamline analysis of heterogeneous-lipid Nanodiscs.

19 biotin transporter into phospholipid bilayer nanodiscs.

20 y in both protein assemblies and lipoprotein Nanodiscs.

21 d fluorescent dyes, DiO and BODIPY, in 10 nm Nanodiscs.

22 al stability of the protein is higher in the nanodiscs.

23 ivated channel TAX-4, reconstituted in lipid nanodiscs.

24 NARE complexes anchored on PIP(2)-containing nanodiscs.

25 DHHC20 is active when reconstituted in POPC nanodiscs.

26 arinic receptor (M2R) reconstituted in lipid nanodiscs.

27 h the inhibitor DCPIB reconstituted in lipid nanodiscs.

28 phatidylserine enhances the binding of MA to nanodiscs.

29 f zebrafish Otop1 and chicken Otop3 in lipid nanodiscs.

30 ions opens new applications for native MS of nanodiscs.

31 een limited by the intermediate stability of nanodiscs.

32 in the native-like lipid environment of the nanodiscs.

33 -that is functionally reconstituted in lipid nanodiscs.

34 constant amount of charge, for POPS and POPC Nanodiscs; 4) in contrast to some previous work, POPC on

35 e preparation of a range of stepwise-smaller nanodiscs (6- to 8-nm diameter) to overcome this limitat

36 The Nanodisc, a nanoscale membrane bilayer disc, is used to

37 -adrenergic receptors reconstituted in lipid nanodiscs activate Src by reducing the lag phase in Src

38 -isolated lipids in purified KcsA-containing nanodiscs allows determination of preferential lipid-pro

39 The chelated nanodiscs also decrease the proton T(1) values for a wat

40 ceptor linkage mechanism itself, building on nanodisc and electron cryotomography experiments.

41 lex AqpZ; a highly polydisperse empty MSP1D1 nanodisc and the tetradecameric chaperone protein comple

42 This inaccessible fraction amounts to 66% in nanodiscs and 82% in micelles.

43 onstrate that a combination of lipid bilayer nanodiscs and a multiplexed silicon photonic analysis te

44 ex with the agonist resiniferatoxin (RTx) in nanodiscs and amphipol.

45 port cycle, we have reconstituted MalFGK2 in nanodiscs and analyzed its conformations under 10 differ

46 eceptors can be reconstituted as monomers in nanodiscs and as tetramers in liposomes.

47 econstituted into two lipid bilayer systems: nanodiscs and bicelles.

48 By combining nanodiscs and cell-free expression technologies, even co

49 e combination of immobilizing the protein in Nanodiscs and footprinting with FPOP is a feasible appro

50 similar structures of NPC1 were obtained in nanodiscs and in detergent at resolutions of 3.6 angstro

51 eractions between the transporter and MBP in nanodiscs and in detergent.

52 he transmembrane domains of mouse TMEM16A in nanodiscs and in lauryl maltose neopentyl glycol as dete

53 on by analyzing the conformation of MalFG in nanodiscs and in proteoliposomes.

54 nds and small diffusing particles, including nanodiscs and liposomes containing membrane protein rece

55 ed on 6 x 6 periodic arrays of both recessed nanodiscs and nanorings to elucidate the differences in

56 constructing membrane-like MCP2901-inserted Nanodiscs and phosphorelay activity assays, we demonstra

57 e complexity of the spectra in heterogeneous Nanodiscs and that the lipid composition can be determin

58 l-2-oleoyl-sn-glycero-3-phosphocholine lipid nanodiscs and the kinetics of activation at 15 degrees C

59 In the presence of nanodiscs and the two ligands, the equilibrium is signif

60 on of functional ion pumps incorporated into nanodiscs and their subsequent analysis by several bioph

61 des effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualiza

62 ed individually in nanoscale lipid patches, "nanodiscs", and directly immobilized on unmodified gold

63 lized within the IM rings in the manner of a nanodisc, and to which the PrgJ rod directly binds and f

64 conflicting observations about the shape of nanodiscs, and pave the way for future integrative studi

65 sium channels, reconstituted them into lipid nanodiscs, and performed single-molecule FRET confocal m

66 rking with large membrane protein systems in nanodiscs, and provide guidelines on the setup of NMR no

67 led with LRET probes, reconstituted in lipid nanodiscs, and the distance between the NBDs was measure

68 In conclusion, nanodiscs are a powerful approach to study the important

69 Lipid bilayer nanodiscs are an attractive tool to study membrane prote

70 the mechanism of formation of polymer-based nanodiscs are characterized by light scattering, NMR, FT

71 Arrays of nanodiscs are common in different types of applications,

72 Nanodiscs are compatible with various techniques, and du

73 Cu(2+) -chelated nanodiscs are demonstrated to reduce the T(1) of protons

74 Lipoprotein nanodiscs are ideally suited for native mass spectrometr

75 Nanodiscs are membrane mimetics that consist of a protei

76 Monodisperse lipid nanodiscs are particularly suitable for characterizing m

77 Lipid nanodiscs are playing increasingly important roles in st

78 These polymer-encased nanodiscs are promising platforms for studies of membran

79 Polymer-based nanodiscs are valuable tools in biomedical research that

80 ngs were compared to the 6 x 6 recessed gold nanodiscs arrays.

81 Utilizing lipid nanodiscs as a membrane mimetic, we show that the MA tri

82 , we not only demonstrated the usefulness of nanodiscs as a membrane-mimicking system, but also showe

83 Development of lipid nanodiscs as a membrane-protein-supporting platform, or

84 highlight the potential of the use of native nanodiscs as a tool in the study of ion channels, and of

85 probed the activities of these enzymes with nanodiscs as membrane mimics to determine whether they c

86 of truncated and full-length TRPV5 in lipid nanodiscs, as well as of a TRPV5 W583A mutant and TRPV5

87 Furthermore, fluorescence anisotropy nanodisc assays revealed a direct physical interaction b

88 Depending on the target protein of interest, nanodisc assembly and purification can be achieved withi

89 sented here are charge measurements on lipid Nanodiscs at 20 degrees C in 100 mM NaCl, 50 mM Tris, at

90 of full-length human TRPM4 embedded in lipid nanodiscs at 3-angstrom resolution, as determined by sin

91 ding cassette exporter MsbA reconstituted in nanodiscs at 37 degrees C while it performs ATP hydrolys

92 ly important property for the development of nanodisc-based characterization methodologies or biotech

93 These nanodisc-based investigations lay the prospects and guid

94 , we utilize two single molecule approaches, nanodisc-based planar bilayer electrophysiology and sing

95 how PI(3,4,5)P3, CaM, and membrane mimetics (nanodisc) bind to Akt(PHD).

96 icroscopy structures of ferroportin in lipid nanodiscs, both in the apo state and in complex with hep

97 e of the beta(1)AR-betaarr1 complex in lipid nanodiscs bound to the biased agonist formoterol(5), and

98 V-ATPase conformations with the structure of nanodisc-bound Vo revealed that Vo is halted in rotation

99 constitution of membrane proteins into lipid nanodiscs by a series of zSMAs with different styrene:ma

100 st aspects of the insertion of proteins into nanodiscs by combining cell-free expression, noncovalent

101 TRPV2 channel in apo and CBD-bound states in nanodiscs by cryo-electron microscopy.

102 dodecyl maltoside (DDM) and in phospholipid nanodiscs by monitoring the spatial positions of transme

103 us of this protocol is on protein NMR, these nanodiscs can also be used for (cryo-) electron microsco

104 Mechanistically, nanodiscs carrying the viral receptor sialic acid bind t

105 We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs

106 ipids or capture the intact membrane protein nanodisc complex-allowing measurement of the membrane pr

107 ties of the slowly tumbling membrane protein-nanodisc complex.

108 oupled receptors in cotranslationally formed nanodisc complexes demonstrate the versatility of this a

109 pMMO-nanodisc complexes with a higher stoichiometry of copper

110 ative MS but also difficult to retain intact nanodisc complexes with membrane proteins inside.

111 e T(1) of protons for both polymer and lipid-nanodisc components.

112 hat is a hybrid between a NPL and an organic nanodisc composed of phospholipids and lipoproteins.

113 ontours of the light-adapted monomeric bR in nanodiscs composed of different lipid ratios exhibited h

114 tocycle intermediates of bR reconstituted in nanodiscs composed of different ratios of the zwitterion

115 h styrene:maleic acid moieties that can form nanodiscs containing a planar lipid bilayer which are us

116 n addition, charge measurements were made on Nanodiscs containing an Escherichia coli lipid extract.

117 ra toxin B subunit homopentamer (CTB(5)) and nanodiscs containing an NGL consisting of the optimal li

118 We demonstrate the feasibility of adsorbing nanodiscs containing KR2 to an artificial bilayer.

119 spectra and lipid stoichiometries of intact Nanodiscs containing lipid-raft associated sphingomyelin

120 Finally, nanodiscs containing the optimal A and B trisaccharide N

121 n of the P450-cytb5 complex in peptide-based nanodiscs, containing no detergents, has been demonstrat

122 rate that high-density lipoprotein-mimicking nanodiscs coupled with antigen (Ag) peptides and adjuvan

123 Here we utilize nanodiscs coupled with native top-down mass spectrometry

124 served between Akt(PHD) and PI(3,4,5)P3-free nanodiscs, demonstrating that PI(3,4,5)P3 is required fo

125 energy transfer measurements in native lipid nanodiscs derived from baby hamster kidney cells, that G

126 (+)-Cl(-) cotransporter (KCC) KCC4, in lipid nanodiscs determined by cryo-EM.

127 dies whereas the magnetic-alignment of macro-nanodiscs (diameter of > ca. 40 nm) can be exploited for

128 ding of synaptotagmin-1 to PIP(2)-containing nanodiscs, disrupting synaptotagmin-1-SNARE interactions

129 the effects of lipid headgroup chemistry on Nanodisc dissociation mechanisms.

130 are largely absent for membrane proteins in nanodiscs due to unfavorable relaxation properties of th

131 Strikingly, nanodiscs elicited up to 47-fold greater frequencies of

132 Nanodiscs eliminated established MC-38 and B16F10 tumour

133 Herein, we report five cryo-EM structures of nanodisc-embedded Ca(v) 1.1 in the presence of the bests

134 roscopy to elucidate the structures of lipid-nanodisc-embedded MsbA in three functional states.

135 Using negative stain electron microscopy and nanodisc-embedding to provide a membrane-like environmen

136 icon photonic microring resonator arrays and nanodiscs enables rapid interrogation of biomolecular bi

137 teraction between small-molecule ligands and nanodisc-encapsulated membrane proteins, because the res

138 kinetics of low molecular mass ligands with nanodisc-encapsulated membrane proteins.

139 een peptide and small-molecule ligands and a nanodisc-encapsulated potassium ion channel protein, Kcs

140 ulating the timing of the kinetics, with the nanodisc environment leading to an earlier Schiff base d

141 t neutral at pH 7.4; 2) high-anionic-content Nanodiscs exhibit polyelectrolyte behavior; 3) 3 mM Ca(2

142 nodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter

143 re-dependent structural changes, the polymer nanodiscs experience negligible structural evolution und

144 rhodopsin dimers and reconstituted them into nanodiscs for cryo-EM analysis.

145 is difficult to eject membrane proteins from nanodiscs for native MS but also difficult to retain int

146 nts and pitfalls relating to optimization of nanodiscs for NMR spectroscopy and outline a strategy fo

147 fundamental bottleneck in the application of nanodiscs for NMR studies.

148 Here we reconstituted Vo into lipid nanodiscs for single-particle EM.

149 es of this system and advantages provided by nanodiscs for structural and mechanistic studies of memb

150 eins can be reconstituted in polymer-encased nanodiscs for studies under near-physiological condition

151 aR-ESI-MS)-based screening, implemented with nanodiscs, for the discovery of GSL ligands.

152 e scaffold proteins (MSP) that do not affect nanodisc formation but shift the masses of nanodiscs in

153 In nanodiscs formed with 1-palmitoyl-2-oleoyl-sn-glycero-3-

154 Phospholipid nanodiscs (formed when a membrane scaffold protein encir

155 well as a rhodopsin dimer reconstituted into nanodiscs from purified monomers.

156 These results suggest that polymer nanodiscs functionalized with paramagnetic tags can be u

157 Nanodiscs generally exhibit more protection of membrane

158 e-detected linear dichroism visualization in Nanodisc grids or SOLVING, to determine the molecular or

159 f lyotropic liquid-crystalline polymer macro-nanodiscs (>20 nm in diameter) as a novel alignment medi

160 t previous native MS of membrane proteins in nanodiscs has been limited by the intermediate stability

161 The structure in nanodiscs has two Ca(2+) ions per monomer and its pore i

162 Recent advances in native MS of lipoprotein nanodiscs have also allowed characterization of antimicr

163 However, transport assays in nanodiscs have not been conducted so far, due to limitat

164 hotonic technology, we constructed arrays of Nanodiscs having variable lipid composition and probed t

165 In nanodiscs, however, an ensemble of pH-dependent conforma

166 Here, we report the discovery of polymer nanodiscs, i.e., discoidal amphiphilic block copolymer m

167 ein, we report structures of BsYetJ in lipid nanodiscs identified by double electron-electron resonan

168 ormations of nhTMEM16 in detergent and lipid nanodiscs illustrate the interactions with its environme

169 t nanodisc formation but shift the masses of nanodiscs in a controllable way, eliminating isobaric in

170 rotein can be reconstituted into the polymer nanodiscs in an active state.

171 oyl-oleoyl-phosphatidylcholine- (POPC-) only nanodiscs in both the unliganded (4.1- angstrom resoluti

172 l YnaI was extracted and delivered in native nanodiscs in closed-like and open-like conformations usi

173 AP complex was purified and reconstituted in nanodiscs in defined stoichiometry.

174 py structures of Mus musculus TASK2 in lipid nanodiscs in open and closed conformations.

175 that membrane protein oligomers ejected from nanodiscs in the gas phase retain large numbers of lipid

176 nt micelles, bicelles, oriented bilayers, or nanodiscs, in order for them to be soluble or dispersed

177 -EM structures of Glt(Ph) reconstituted into nanodiscs, including those structurally constrained in t

178 Here, we develop a nanodisc incorporated with a decoy virus receptor that i

179 In summary, nanodisc-incorporated human SQR exhibits enhanced cataly

180 y showed that the negative surface charge of nanodiscs increased as the content of DOPG or DMPG was i

181 eptors, as higher loading of Aer dimers into nanodiscs increases kinase activity.

182 ow that Akt(PHD) binds to both layers of the nanodisc, indicating proper incorporation of PI(3,4,5)P3

183 The nanodiscs inhibit influenza virus infection and reduce m

184 Although it is commonly agreed that the nanodisc is plain disk shaped, several more advanced str

185 id-bilayer presentation of viral antigens in Nanodiscs is a new platform for evaluating neutralizing

186 alpha1beta3gamma2L GABA(A) receptor in lipid nanodiscs is bound to the channel-blocker picrotoxin, th

187 Our results reveal that BsYetJ in lipid nanodiscs is structurally different from those crystalli

188 sidue-specific reassignment of resonances in nanodiscs is therefore extremely time and sample consumi

189 show that the conformation of KcsA in native nanodiscs is very similar to that in detergent micelles,

190 cyclic proteins to aid in the development of nanodisc membrane mimetics.

191 , we used single-molecule FRET assays with a nanodisc membrane reconstitution system to investigate t

192 limitations of the two-dimensional nature of nanodisc membranes that offers no compartmentalization.

193 bound to the human immune receptor CD59 in a nanodisc model membrane.

194 odopsin-octylglucoside micelle and the empty nanodisc (MSP1D1-Nd) using both MS and tandem-MS modes o

195 mics and biophysical properties of two small nanodiscs, MSP1D1DeltaH5 and DeltaH4H5.

196 nductance mechanosensitive channel (MscS) in nanodiscs (ND).

197 Nanodiscs (NDs) are an excellent alternative to small un

198 We investigated this phenomenon in lipid nanodiscs (NDs) at equilibrium on a local scale, analyzi

199 with the CaR-ESI-MS assay implemented using nanodiscs (NDs) revealed that the PDs exhibited similar

200 y (ESI-MS) analysis combined with the use of nanodiscs (NDs) to solubilize glycolipids (GLs) has rece

201 d-release (CaR)-ESI-MS assay, which exploits nanodiscs (NDs) to solubilize glycolipids and mimic thei

202 aloganglioside ligand GM1, incorporated into nanodiscs (NDs), for cholera toxin B subunit homopentame

203 Here, we embedded human SQR in nanodiscs (ndSQR) and studied highly homogenous preparat

204 oscopy of membrane proteins in commonly used nanodiscs of 10-nm diameter were limited by the high mol

205 maleic acid (SMA) is a polymer that extracts nanodiscs of biological membranes (containing membrane p

206 X-MS) of membrane proteins incorporated into nanodiscs of controlled lipid composition is used to obt

207 to solubilize membrane proteins and produce nanodiscs of controlled sizes.

208 servation of the tertiary structure of bR in nanodiscs of different lipid compositions.

209 A remarkable feature is that nanodiscs of different sizes, from nanometer to sub-micr

210 s of p75NTR, incorporated into lipid-protein nanodiscs of various sizes and compositions, by solution

211 t a protocol on the assembly of phospholipid nanodiscs of various sizes for structural studies of mem

212 The incorporation efficiencies (into nanodiscs) of the NGLs and their affinities for a fragme

213 fined lipid bilayer environment, lipoprotein Nanodiscs offer a promising cassette for membrane protei

214 Our results demonstrate that nanodiscs offer the potential for native mass spectromet

215 ly detergent micelles but also lipid bilayer nanodiscs or bicelles can serve as a means for the gentl

216 complex is stabilized by incorporation into nanodiscs or bicelles; (iv) removal of bound phospholipi

217 ent protein that had been reconstituted into nanodiscs or proteoliposomes.

218 Additionally, nanodiscs permitted us to visualize two distinct TRPV2 a

219 However, native mass spectrometry of nanodiscs produces complex spectra that can be challengi

220 ic amounts relative to binding sites) in the nanodisc promotes GD1b binding to CTB(5); no GD1b bindin

221 Nanodiscs provide a new and powerful tool for a broad sp

222 Lipoprotein nanodiscs provide a platform to present membrane protein

223 The cell-free synthesis in combination with nanodiscs provides a defined hydrophobic lipid environme

224 By employing differently sized nanodiscs reconstituted with single SecYEG complexes and

225 We determined the cryo-EM structure of nanodisc-reconstituted human ABCB4 trapped in an ATP-bou

226 oelectron microscopy (cryo-EM) structures of nanodisc-reconstituted ligand-free TRPA1 and TRPA1 in co

227 As with lipid nanodiscs, reconstitution of detergent-solubilized MsbA

228 membrane protein, essential for liposome or nanodiscs reconstitutions.

229 ures of human TRPV3 reconstituted into lipid nanodiscs, representing distinct functional states durin

230 PECAM-1-containing nanodiscs retained not only their ability to bind homoph

231 sides of the transporter reconstituted into nanodiscs reveal large-amplitude movement of helices tha

232 rmore, MPER-TMD assembly into 10-nm diameter nanodiscs revealed a heterogeneous membrane array compri

233 pid distribution toward the membrane protein nanodiscs revealed lipid binding, and titrations allowed

234 e-bound human ABCB1 reconstituted in lipidic nanodiscs, revealing a single molecule of the chemothera

235 At low pH in the endosome, the nanodiscs rupture the viral envelope, trapping viral RNA

236 detergent-free synthesis of membrane protein/nanodisc samples and the analysis by LILBID mass spectro

237 The solid-supported membrane assay with nanodisc samples provides reliable control over the ioni

238 For both proteins, the kinetics in nanodiscs shows the characteristics observed in the nati

239 detergent-solubilized MsbA into the polymer nanodiscs significantly enhances its activity.

240 To simplify interpretation of nanodisc spectra, we engineered a series of mutant membr

241 rgent assignment could be transferred to the nanodisc spectrum.

242 Furthermore, experiments using nanodiscs strongly suggest that autotransporter assembly

243 uting unlabeled LeuT in phospholipid bilayer nanodiscs, subjecting them to hydrogen-deuterium exchang

244 ulations performed on ganglioside-containing nanodiscs suggest that the participation of low affinity

245 its activation in cells as well as in lipid nanodiscs, suggesting that physical deformation of the l

246 g proper incorporation of PI(3,4,5)P3 on the nanodisc surface.

247 Here, using the nanodisc system and biolayer interferometry assays, we f

248 the SAXS data from the N-terminal truncated nanodisc system upon cholesterol incorporation.

249 50) intercellular channels into a dual lipid nanodisc system, mimicking a native cell-to-cell junctio

250 lar to the particles formed in the so-called nanodisc system, which is based on N-terminal truncated

251 detailed comparative study of the ApoA1 and nanodisc systems upon cholesterol uptake.

252 of fluorescence correlation spectroscopy and nanodisc technologies provides a powerful approach to ac

253 0 in the human heart, using a combination of Nanodisc technology and a nanohole plasmonic sensor call

254 Nanodisc technology provides membrane proteins with a na

255 ombining electron cryo-microscopy with lipid nanodisc technology to ascertain the structure of the ra

256 omprehensive list of various applications of nanodisc technology with systematic analysis of the most

257 combination of cell-free synthetic biology, nanodisc-technology and non-covalent mass spectrometry p

258 lyR protein complex reconstituted into lipid nanodiscs that are captured in the unliganded (closed),

259 Nanodiscs that hold a lipid bilayer surrounded by a boun

260 In contrast to lipid nanodiscs that undergo time- and temperature-dependent s

261 In nanodiscs, the ensemble of substates in the photoactivat

262 The easy preparation of macro-nanodiscs, their high stability against pH changes and t

263 , binding of a PI(3,4,5)P3-embedded membrane nanodisc to Akt(PHD) with a 10(3)-fold tighter affinity

264 ed the structure of the human EMC in a lipid nanodisc to an overall resolution of 3.4 angstroms by cr

265 iors from nonspecific incorporation into the nanodisc to formation of specific complexes.

266 ering pores connecting v-SNARE-reconstituted nanodiscs to cells ectopically expressing cognate, "flip

267 The ability of nanodiscs to trap amyloid intermediates as demonstrated

268 The dramatic differences in the stability of nanodiscs under different ESI conditions opens new appli

269 the native-like environment of phospholipid nanodiscs undergo spontaneous transitions between two di

270 The small-size nanodiscs (up to ca. 30 nm diameter) can be used for sol

271 icant amounts of lipid are released from the nanodiscs upon insertion of larger protein complexes.

272 onstration of a protein-protein complex in a nanodisc using NMR structural studies and should be usef

273 ning of isotope-labeled membrane proteins in nanodiscs using nuclear Overhauser enhancement spectrosc

274 loped a system to study KRAS dimerization on nanodiscs using paramagnetic relaxation enhancement (PRE

275 to ganglioside mixtures in model membranes (nanodiscs) using native mass spectrometry (MS) and compe

276 e discoidal proteolipid particles or "native nanodiscs." Using circular dichroism and tryptophan fluo

277 can be transferred to a spectrum recorded in nanodiscs via detergent titration.

278 FERONIA protein kinase activity in nanodiscs was higher than that of soluble protein and co

279 cardiolipin, assembly of these proteins into nanodiscs was initiated.

280 d, alone or present together with GM1 in the nanodiscs, was observed.

281 reconstituted into vacuolar lipid-containing nanodiscs, we further demonstrate that disruption of H(C

282 gle FoF1 molecules embedded in lipid bilayer nanodiscs, we now report the observation of Fo-dependent

283 asurements of F0F1 embedded in lipid bilayer nanodiscs, we observed that the ability of the F0 motor

284 The nanodiscs were formed with major scaffold protein (MSP)

285 hydrolysis, the NBDs of Pgp reconstituted in nanodiscs were never far apart during the hydrolysis cyc

286 ful preparation of novel peptide-based lipid nanodiscs, which are detergent-free and possesses size f

287 ical oxidation of proteins (FPOP), and lipid Nanodiscs, which are more similar to the native membrane

288 o planar lipid bilayers directly from native nanodiscs, which enables functional characterization of

289 Chemoreceptors were inserted in Nanodiscs, which rendered them water soluble and allowed

290 brane that maintains the advantages of lipid nanodiscs while addressing their weaknesses.

291 (styrene-co-maleic acid) polymer which forms nanodiscs while showing the ability to chelate metal ion

292 n alphaIIbbeta3 embedded in a lipid bilayer (nanodiscs) while bound to domains of the cytosolic regul

293 e the exchange of lipids between lipoprotein nanodiscs with and without an embedded membrane protein.

294 rotein complex in detergent, and lipoprotein nanodiscs with and without embedded peptides, and used c

295 stabilize, and support membrane proteins in nanodiscs with different efficiencies depending on both

296 ichiometry of AMPs inserted into lipoprotein nanodiscs with different lipid components.

297 inally, we demonstrate the use of mixed belt nanodiscs with embedded membrane proteins to confirm the

298 nondenaturing MS to ionize membrane protein nanodiscs with heterogeneous lipids.

299 K(d)) for each coagulation factor binding to Nanodiscs with unique compositions of PE and PS were det

300 irs of spin labels in MdfA, reconstituted in nanodiscs, with cysteine cross-linking of natively expre

301 genous membrane:protein assemblies in native nanodiscs without exposure to conventional detergents th