ライフサイエンスコーパス: nanopore sequencing

1 ngle-nucleotide differences (microarrays and nanopore sequencing).

2 46, ~4-21 million sites via TWIST and Oxford Nanopore sequencing).

3 obtained from clinical samples using R9.4.1 Nanopore sequencing.

4 transport of DNA is essential for realizing nanopore sequencing.

5 es solutions to two long-standing hurdles to nanopore sequencing.

6 ight the importance of MspA in the future of nanopore sequencing.

7 combining rolling-circle amplification with Nanopore sequencing.

8 on, confirmed in mature mRNAs by direct cDNA nanopore sequencing.

9 DNA from clinical samples were sequenced by Nanopore sequencing.

10 omic data with genomic skimming and portable nanopore sequencing.

11 ified and sequenced full-length eccDNAs with Nanopore sequencing.

12 d to type a subset of all SVs using targeted nanopore sequencing.

13 ute to the discrepancy between bisulfite and nanopore sequencing.

14 on detection using ionic current signal from Nanopore sequencing.

15 xtraction and library preparation for Oxford Nanopore sequencing.

16 ites on mammalian mRNAs via direct long-read nanopore sequencing.

17 using BrdU incorporation and single-molecule nanopore sequencing.

18 by integration of the pan-flavivirus PCR and Nanopore sequencing.

19 asecalling models for commercially available nanopore sequencing.

20 ween reduced representation and whole-genome Nanopore sequencing.

21 us generation for closely related genes with nanopore sequencing.

22 hroughput workflows that are compatible with Nanopore sequencing.

23 mammalian whole-genome DNA modifications in nanopore sequencing.

24 le-molecule DNA sequencing platforms such as Nanopore sequencing.

25 Samples from 2023 were subjected to nanopore sequencing.

26 fied before off-chip transfer and downstream nanopore sequencing.

27 oligonucleotide, followed by high throughput nanopore sequencing.

28 and 91 and 100% for fungi, respectively, by nanopore sequencing.

29 ng, and demonstrate retrieval with streaming nanopore sequencing.

30 that drastically increases the throughput of nanopore sequencing.

31 g Illumina and compare the results to Oxford Nanopore sequencing.

32 ficient saponin-based host DNA depletion and nanopore sequencing.

33 the samples (n = 19) by Sanger and by Oxford Nanopore sequencing.

34 mosomal DNA with Cas9 to ligate adapters for nanopore sequencing.

35 in biotechnological applications such as DNA nanopore sequencing(2-4), resulting in considerable inte

36 Nanopore sequencing allows us to identify a large variet

37 combination of isothermal amplification and Nanopore sequencing also offers appealing potential to d

38 Sanger and nanopore sequencing analysis reveals significant variati

39 To address these gaps, we use long-read nanopore sequencing and assemble the genomes of two circ

40 splay the DNA methylation levels detected by nanopore sequencing and bisulfite sequencing data across

41 ltant RNA molecules can be directly read via nanopore sequencing and can also be enzymatically comput

42 ry to other methods to boost the accuracy of nanopore sequencing and could be incorporated into any e

43 quencing applications, from DNA isolation to nanopore sequencing and downstream data analysis, all of

44 , we use polymerase-free, targeted long-read nanopore sequencing and evaluate single- and dual-gRNA A

45 Here, we use nanopore sequencing and Hi-C scaffolding to produce de n

46 MYCN amplicon structure using short-read and Nanopore sequencing and its chromatin landscape using Ch

47 cimen-to-result protocol that uses long-read nanopore sequencing and machine learning to characterize

48 We applied nanopore sequencing and our workflow, named Lathe, which

49 RNA 2'-O-methyl (Nm) modification mapping by nanopore sequencing and the effect of Nm on mRNA stabili

50 Harnessing real-time nanopore sequencing and the high-quality kakapo referenc

51 c patterns were retrieved high-throughput by nanopore sequencing, and algorithms were developed to fi

52 additional genetic data, including long-read nanopore sequencing, and clinical variables.

53 Improvements in Oxford Nanopore sequencing, and development of novel computatio

54 wo bases can be mixed as a single sample for nanopore sequencing, and routine multiplexing of even si

55 and a Chlamydomonas reinhardtii genome using Nanopore sequencing, and then evaluate DeepMod on three

56 developed an assembly-free, single-molecule nanopore sequencing approach, enabling direct recovery o

57 cBio circular consensus sequencing (CCS) and nanopore sequencing, are advantageous in detecting DNA 5

58 Here I present nanopore sequencing as a highly precise platform for glo

59 We show that this product is suitable for Nanopore sequencing as single reads, as well as for gene

60 We also demonstrate the potential of nanopore sequencing assemblies for rapid preliminary phy

61 a virus using partial gene amplification and nanopore sequencing backed up the use of the recombinant

62 paradigm for building modification-tolerant nanopore sequencing basecallers.

63 we introduce Nanotiming, a single-molecule, nanopore sequencing-based method producing high-resoluti

64 nces at a median accuracy of 97.9% using our nanopore sequencing-based Rolling Circle Amplification t

65 h various algorithms have been developed for nanopore-sequencing-based modification analysis, more de

66 fications remains a substantial challenge in nanopore sequencing bioinformatics.

67 -time sequencing by Pacific Biosciences, and nanopore sequencing by Oxford Nanopore Technologies.

68 Our results indicate that nanopore sequencing can be a suitable alternative to, or

69 These results demonstrate that nanopore sequencing can be used to deconvolute individua

70 Oxford Nanopore sequencing can detect DNA methylations from ion

71 We investigated if Nanopore sequencing can detect sufficient Neisseria gono

72 ients with surgically treated osteomyelitis, nanopore sequencing can generate interpretable metagenom

73 es in non-bone infections have revealed that nanopore sequencing can provide real-time metagenomic id

74 Our findings show that metagenomic Nanopore sequencing can provide reliable diagnostic info

75 Long-read sequencers, such as nanopore sequencing, can address this problem by providi

76 ONT recently upgraded their Nanopore sequencing chemistry and kits from the R9 to th

77 e a CRISPR-Cas-based enrichment strategy for nanopore sequencing combined with an algorithm for raw s

78 cost-effective method uses cDNA pulldown and Nanopore sequencing combined with an analysis pipeline t

79 Targeted nanopore sequencing complements recently developed molec

80 Nanopore sequencing confirmed the lower transcription of

81 Nanopore sequencing confirms that long linear chains are

82 suggest that sequencing the IGS region using nanopore sequencing could be a potential new molecular d

83 separate species, which we wanted to see if nanopore sequencing could detect.

84 Metagenomic nanopore sequencing coupled with human DNA depletion has

85 ts show that unlike established diagnostics, nanopore sequencing data analysis can accurately detect

86 w for evaluating aligners and SV callers for nanopore sequencing data and approaches for integrating

87 rithm that identifies and corrects errors in nanopore sequencing data and improves the accuracy of de

88 e modifications on native RNA molecules from nanopore sequencing data and multiple modifications on t

89 e aim of this study was to determine whether Nanopore sequencing data can provide equivalent informat

90 methods for detecting DNA modification from nanopore sequencing data do not effectively support de n

91 Here, we used publicly available nanopore sequencing data for 12 standard B-lymphocyte ce

92 Nanopore sequencing data from a single 18-h run was used

93 Detecting the SNP variants on low-coverage Nanopore sequencing data is still a challenging problem.

94 e describe the generation of a comprehensive nanopore sequencing data set with a median read length o

95 ConSeqUMI processes nanopore sequencing data without the need for reference

96 noCaller in identifying SNPs on low-coverage Nanopore sequencing data, including the difficult-to-map

97 te, organize, summarize and visualize MinION nanopore sequencing data.

98 and cloud compatible pipeline for analyzing Nanopore sequencing data.

99 Pore, a toolkit for exploring and analysing nanopore sequencing data.

100 e algorithm and tested it on both PacBio and Nanopore sequencing datasets.

101 Direct molecular detection and nanopore sequencing (DDNS) of poliovirus in stool sample

102 ing on desired fragment length and 1-3 d for Nanopore sequencing depending on desired sequencing dept

103 In addition, Nanopore sequencing detected more positive samples (n =

104 Nanopore sequencing detected the same variants as identi

105 using lossy compression, potentially on the nanopore sequencing device itself, to achieve significan

106 The Oxford Nanopore sequencing device that we used is cost-effectiv

107 uld be incorporated into any enzyme-actuated nanopore sequencing device.

108 Nonetheless, Nanopore sequencing devices are limited in the number of

109 toolkit for exploring datasets generated by nanopore sequencing devices from MinION for the purposes

110 High-throughput third-generation nanopore sequencing devices have enormous potential for

111 In nanopore sequencing devices, electrolytic current signal

112 increasing the electrophoretic force used in nanopore sequencing devices.

113 center of an unfolding outbreak, metagenomic nanopore sequencing directly from patient samples, an ap

114 CRISPR-CATCH followed by nanopore sequencing enabled single-molecule ecDNA methyl

115 Here, we evaluate the extent to which Nanopore sequencing enables detection and analysis of so

116 Nanopore sequencing enables direct, single-molecule inte

117 The Oxford Nanopore sequencing enables to directly detect methylati

118 this approach, we successfully applied it to Nanopore sequencing, enabling ultra-fast analysis of nov

119 Nanopore sequencing errors were predominantly in regions

120 consistent with results of state-of-the-art nanopore sequencing experiments.

121 ompression algorithm especially designed for nanopore sequencing FASTQ files.

122 end-to-end solution of 16S rRNA gene Oxford Nanopore sequencing for bacterial isolate identification

123 r work demonstrates the potential utility of nanopore sequencing for cancer and splicing research.

124 strates the feasibility and utility of using nanopore sequencing for comprehensive viral monitoring i

125 Here we assess the viability of using Nanopore sequencing for epidemiology by performing a com

126 ic spacer (IGS) sequence in combination with nanopore sequencing for fungal identification.

127 -sample sequencing cost and hands-on time of Nanopore sequencing for hybrid assembly by at least 50%

128 Our study demonstrates the feasibility of nanopore sequencing for malaria genomic surveillance in

129 This work introduces the utility of nanopore sequencing for MEI enrichment and lays the foun

130 It therefore expands the utility of nanopore sequencing for molecular diagnostics and other

131 Our study illustrates the accuracy of Nanopore sequencing for rapid and field prion disease di

132 This study also demonstrates the utility of nanopore sequencing for the characterization of dynamic

133 also the first report on the application of nanopore sequencing for the kinetic characterization of

134 ever, the recent technological innovation of nanopore sequencing from Oxford Nanopore Technologies (O

135 hyl Capture EPIC), single-molecule long-read nanopore sequencing from Oxford Nanopore Technologies, a

136 Nanopore sequencing generates noisy electrical signals t

137 Canu introduces support for nanopore sequencing, halves depth-of-coverage requiremen

138 Nanopore sequencing has been available to researchers fo

139 Nanopore sequencing has emerged as a major sequencing te

140 Oxford Nanopore sequencing has great potential and advantages i

141 Nanopore sequencing has revolutionized genetic analysis

142 Nanopore sequencing has the potential to become a direct

143 Many errors induced by nanopore sequencing have a bias because of the physics o

144 Most recently, new technologies such as nanopore sequencing have reduced both cost and equipment

145 ad DNA sequencing technologies, specifically Nanopore sequencing, have made possible the rapid identi

146 eneration sequencing technologies, including Nanopore sequencing, have the potential to revolutionize

147 In retrospective cohorts profiled by nanopore sequencing, high-confidence predictions were co

148 ies and challenges to implementing MMS using nanopore sequencing, highlighting priority areas for tec

149 Real-time genomics through nanopore sequencing holds the promise of fast antibiotic

150 ning inverted duplicated DNA sequences using nanopore sequencing identified recurrent aberrant behavi

151 Nanopore sequencing identified the same cultured organis

152 However, the high intrinsic error rate of nanopore sequencing impedes the analysis of complex hete

153 By combining long-range PCR and nanopore sequencing in 169 patients with cerebellar atax

154 analysis enabled pathogen identification by nanopore sequencing in a median 50-min sequencing and 6-

155 to date supports the potential future use of nanopore sequencing in infectious disease diagnostics.

156 One such method is nanopore sequencing, in which the delivery of biomolecul

157 ecoded from the Oxford Nanopore Technologies nanopore-sequencing ionic current signals.

158 ial resistance determinants from error-prone Nanopore sequencing is a substantial bioinformatics chal

159 In summary, Nanopore sequencing is a valid option for identifying ge

160 st that reliable detection of non-B DNA from nanopore sequencing is achievable.

161 Oxford Nanopore sequencing is an efficient and low-cost platfor

162 Nanopore sequencing is being applied in genome assembly,

163 Nanopore sequencing is one of the most promising technol

164 Nevertheless, the current cost of nanopore sequencing is still prohibitive for routine seq

165 Nanopore sequencing is well-suited for this purpose, as

166 Nanopore sequencing may be the next disruptive technolog

167 n aspects of the physical process underlying nanopore sequencing mean that some sequences are more pr

168 her optimization and evaluation of the rapid nanopore sequencing method could potentiate the widening

169 btained and analysed using newly established nanopore sequencing methodology and bioinformatic pipeli

170 rrays of nanopores are required, but current nanopore sequencing methods rely on ionic current measur

171 ncrease in throughput compared with existing Nanopore sequencing methods.

172 This highlights the need to optimize nanopore sequencing motors to handle different glycosidi

173 Improvements in nanopore sequencing necessitate efficient classification

174 ication of nervous system malignancies using nanopore sequencing not only for the neurosurgical intra

175 AF single nucleotide variant mutations using Nanopore Sequencing, OCEANS is poised to enable same-day

176 Here, we take the first steps to realize nanopore sequencing of an 8-letter "hachimoji" expanded

177 Here, we use deep nanopore sequencing of Bsal and comparative genomics aga

178 Here we demonstrate de novo nanopore sequencing of DNA comprised entirely of the fou

179 ng the ionic current signals produced during nanopore sequencing of DNA containing noncanonical XNA b

180 Nanopore sequencing of DNA is a single-molecule techniqu

181 Direct nanopore sequencing of DNA provides a means to detect al

182 Here we perform nanopore sequencing of DNA-barcoded molecular probes eng

183 ndividual identification is feasible through nanopore sequencing of environmental DNA, with important

184 We report nanopore sequencing of full-length cDNA from CLL samples

185 Single-cell nanopore sequencing of full-length mRNAs transforms sing

186 This work provides a foundation for nanopore sequencing of long, natural DNA strands.

187 Based on whole genome nanopore sequencing of NA12878, we show that there exist

188 We performed nanopore sequencing of nucleosome occupancy and methylom

189 ere, we demonstrate an approach for targeted nanopore sequencing of P. falciparum from dried blood sp

190 Nanopore sequencing of proteins was also envisioned.

191 r transcriptomes were analyzed by RNA direct nanopore sequencing of ribosomal RNA and chromatography

192 eotide sequences and permits standalone cDNA nanopore sequencing of single cells.

193 neralizable to any application that requires nanopore sequencing of small DNA amplicons.

194 as enabled by high-coverage, ultra-long-read nanopore sequencing of the complete hydatidiform mole CH

195 Using Nanopore sequencing of urine samples from men with ureth

196 Direct RNA Nanopore sequencing of Wolbachia-cured lines identified

197 Nanopore sequencing offers a new, portable method to det

198 Nanopore sequencing offers a portable and affordable alt

199 Nanopore sequencing offers considerable flexibility by a

200 ucks, mice, and humans, as well as long-read nanopore sequencing on diverse chicken breeds, we find t

201 fic methylation using single-molecule Oxford Nanopore Sequencing (ONS).

202 R-Cas9 genome editing tool combined with the Nanopore Sequencing (ONT) we showed that the 7-deazaguan

203 Thus, Nanopore sequencing operated with 83% sensitivity (95% c

204 y (isothermal amplification, microarrays and nanopore sequencing) or specificity for single-nucleotid

205 Objectives: We sought to develop a Nanopore sequencing panel and validate its performance i

206 Methods: We developed a Nanopore sequencing panel targeting 15 SNPs in five gene

207 , particularly those derived from the Oxford Nanopore sequencing platform, tend to exhibit high error

208 fragments of synthetic DNA using a portable nanopore sequencing platform.

209 of software-controlled enrichment unique to nanopore sequencing platforms.

210 Here we present RNA structure analysis using nanopore sequencing (PORE-cupine), which combines struct

211 thms in order to fully utilize the extent of nanopore sequencing potential.

212 Oxford Nanopore sequencing producing long reads at low cost has

213 Nanopore sequencing promises long read-lengths and singl

214 We present a nested PCR and nanopore sequencing protocol that allows rapid (<3 days)

215 Nanopore sequencing proved particularly valuable for ide

216 Quasi-metagenomics with nanopore sequencing provided thousands of high-contiguit

217 Nanopore sequencing provides a real-time and portable so

218 Nanopore sequencing provides additional advantages as ve

219 PoreSeq increases nanopore sequencing read accuracy of M13 bacteriophage D

220 leotide kmers are commonly used in analyzing nanopore sequencing readouts.

221 the number of nucleotide modifications from nanopore-sequencing readouts.

222 ains were assembled from hybrid Illumina and Nanopore sequencing reads and annotated.

223 Furthermore, we show nanopore sequencing reads of phi X 174 up to 4,500 bases

224 Map, a mapping algorithm designed to analyse nanopore sequencing reads, which progressively refines c

225 een developed to detect DNA methylation from nanopore sequencing reads.

226 ism (SNP) callers are aimed at high-coverage Nanopore sequencing reads.

227 ork direction, and termination on individual nanopore sequencing reads.

228 method to detect DNA methylation states from Nanopore sequencing reads.

229 cost-effective DNA library preparation with nanopore sequencing, reducing reliance on extensive thir

230 Because nanopore sequencing relies on a molecular motor to contr

231 Realizing the democratic promise of nanopore sequencing requires the development of new bioi

232 mosome analysis/cytogenetics, short molecule nanopore sequencing returns more sensitive, accurate cop

233 Recently, portable, real-time, nanopore sequencing (RTnS) has become available.

234 oV-2 genome sequences from a single portable Nanopore sequencing run, representing a threefold increa

235 idization, whole-genome, target-enriched and nanopore sequencing, sequence alignment and variant dete

236 In contrast, PCR-based nanopore sequencing showed bias for partial AAV fragment

237 ated-DNA immunoprecipitation sequencing, and nanopore sequencing showed that IFITM1 and ISG15-classic

238 Nanopore sequencing shows utility as a rapid surveillanc

239 In this work, we observed that a nanopore sequencing signal displays complex heterogeneit

240 n basecalling-the process of translating raw nanopore sequencing signals into DNA base sequences usin

241 cheme that leverages soft information in raw nanopore sequencing signals to achieve information readi

242 y underestimated expansion sizes compared to nanopore sequencing (slope, 0.87 [95% CI, 0.81 to 0.93];

243 imilar between capillary electrophoresis and nanopore sequencing (slope: 0.98 [95% CI, 0.92 to 1.04];

244 recent improvements in basecalling accuracy, nanopore sequencing still has higher error rates on shor

245 is study establishes a rationale for further nanopore-sequencing studies of heterogeneous cell popula

246 ng methods eliminate many of the benefits of nanopore sequencing, such as the ability to accurately d

247 ility of the use of emerging single-molecule nanopore sequencing techniques for these purposes.

248 However, nanopore sequencing technologies are rapidly gaining pop

249 nces in single molecule real-time (SMRT) and nanopore sequencing technologies have enabled high-quali

250 Emerging Nanopore sequencing technologies offer the potential for

251 Continued development of nanopore sequencing technologies will likely enhance the

252 This review will examine current nanopore sequencing technologies, including recent devel

253 The emerging nanopore sequencing technology can generate long sequenc

254 Nanopore sequencing technology has the potential to rend

255 The emergence of nanopore sequencing technology has the potential to tran

256 Recent advances in nanopore sequencing technology have led to a substantial

257 Overall, this paper highlights the use of Nanopore sequencing technology in combination with the A

258 Here, we use long-read nanopore sequencing technology to develop a crossover ma

259 Prior to this, nanopore sequencing technology was mainly used to analyz

260 circumvented the expected error rate of the nanopore sequencing technology, producing a genome seque

261 Inspired by nanopore sequencing technology, we developed a plasmonic

262 nostics workflow centred around the portable nanopore sequencing technology.

263 ast, to overcome the high error rates of the nanopore sequencing technology.

264 l role in overcoming the high error rates of nanopore sequencing technology.

265 g optical mapping and cas9-assisted targeted nanopore sequencing to analyze SVs.

266 We used long-read nanopore sequencing to capture 238,490 SVs in 100 divers

267 Here, we have used whole-genome Nanopore sequencing to characterize several CGRs that or

268 For evaluation, we used nanopore sequencing to classify over 200 brain tumor sam

269 apply new computational tools and long-read nanopore sequencing to directly infer CpG methylation of

270 m(6)A) methyltransferase, and the ability of nanopore sequencing to directly read DNA modifications.

271 ensus calling based on genome-wide long-read nanopore sequencing to enable simultaneous multimodal tu

272 nd SNV-calling methodology must continue for nanopore sequencing to establish itself as a primary met

273 In this study, we use nanopore sequencing to evaluate CpG methylation and chro

274 cule magnetic tweezers, gel-based assays and nanopore sequencing to explore DNA unwinding and cleavag

275 ategy in which we use programmable selective nanopore sequencing to focus LRS data production onto th

276 knowledge of imprinting and the potential of nanopore sequencing to identify imprinting regions using

277 Here, we used direct RNA nanopore sequencing to investigate allele-specific pre-m

278 gate, our results demonstrate the utility of nanopore sequencing to pinpoint CGRs associated with ASD

279 Here we apply Oxford Nanopore sequencing to profile 6mA at base-pair resoluti

280 Here, we apply targeted nanopore sequencing to profile chromatin accessibility a

281 ra-fast 84-second LC-MS method, and barcoded nanopore sequencing to rapidly isolate and characterise

282 DNA barcodes enable Oxford Nanopore sequencing to sequence multiple barcoded DNA sa

283 Here, we used long-range PCR and Nanopore sequencing to sequence the full length of PRNP,

284 Here, we report the use of long-read nanopore sequencing to simultaneously sequence the entir

285 deploy digital telomere measurement (DTM) by nanopore sequencing to understand how distributions of h

286 Here, we used nanopore sequencing to validate published complete genom

287 les cells for both short-read and long-read (nanopore) sequencing together with a new computational p

288 0 correlations with bisulfite sequencing and nanopore sequencing using only 10x reads.

289 Seq, HiSeq 2500, or NextSeq500 platforms and Nanopore sequencing was done on the MinION or GridION pl

290 Nanopore sequencing was instrumental to study the compos

291 Using long-read nanopore sequencing we show that, with an average genomi

292 By linking this amplification step with nanopore sequencing, we identified >95% annotated L1s fr

293 verse transcriptase, or "RT stops"; and (ii) nanopore sequencing where the cross-link produces mutati

294 One such application is nanopore sequencing, where a DNA molecule is electrophor

295 loop-mediated isothermal amplification with nanopore sequencing, which could potentially be used to

296 this challenge, combining long-range PCR and nanopore sequencing with a novel bioinformatics pipeline

297 sults provide a framework for short-molecule nanopore sequencing with applications in research and me

298 Here, we pair Cas9-targeted nanopore sequencing with computational methodologies to

299 We find that nanopore sequencing with Hi-C scaffolding produces highl

300 Combining nanopore sequencing with our developed machine learning