ライフサイエンスコーパス: native PAGE

1 ize of <15 kDa by gel chromatography; and 4) native PAGE of AF yields a band of high apparent m.w., a

2 We adapted a native PAGE method to assess the formation and thermosta

3 mobility of the resulting RNP complexes in a native PAGE assay to determine which portions of these S

4 0-100% conversion of octamer to hexamer in a native PAGE mobility shift assay.

5 hese glycoproteins migrate as a complex in a native PAGE system.

6 Reaction stoichiometry analyses and native PAGE showed that for every factor Xa molecule inh

7 cattering, size exclusion chromatography and native PAGE show that Hs11S is assembled in different ol

8 ipitation, cross-linking, and denaturing and native PAGE analyses of Gal80 in vitro and fluorescence

9 Pull-down experiments and native PAGE analysis indicated that the protein is both

10 activity, hydroxyl radical footprinting, and native PAGE.

11 crosomes because both isoelectrofocusing and native PAGE indicate the presence of a second region of

12 in mitoplasts and holo-CI by non-native and native PAGE, respectively, to determine their CI incorpo

13 inding, NMR titration, alanine scanning, and native PAGE.

14 -phase microextraction (SPME) GC-MS, SDS and native PAGE gel electrophoresis demonstrated that the ov

15 Analytical ultracentrifugation and native PAGE studies indicate that HSBP1 is predominantly

16 molecular mass estimated, using SDS-PAGE and native-PAGE electrophoresis, to be 86kDa.

17 ith high purity, as assessed by SDS-PAGE and native-PAGE zymogram, appearing as a single band of appr

18 redoxin (a-Fd) using UV-VIS spectroscopy and native-PAGE, as well as with a mitochondrial iron detect

19 Blue native PAGE analyses verify the presence of a SecYEG-Ppi

20 Blue native PAGE analysis demonstrates that RESC14 is essenti

21 Blue native PAGE analysis identified high molecular weight co

22 Blue native PAGE analysis indicates that native H complexes e

23 Blue native PAGE and FRET assays revealed 1% n-dodecyl beta-d

24 Blue native PAGE and immunoblot analysis revealed a single ba

25 Gel filtration analysis and blue native PAGE showed that Cbp1 is part of a single 900,000

26 ory chain proteins by Western blotting, blue native PAGE, and enzymatic activity assays revealed that

27 of cytochrome-c oxidase was detected by blue native PAGE, and interaction between subunit IV of cytoc

28 Finally, blue native PAGE (BN-PAGE) revealed that under nondenaturing

29 A knockdowns, pharmacologic inhibitors, blue native PAGE, mass spectrometry, and assessment of mitoch

30 re resolved using two-dimensional PAGE (blue native PAGE and tricine/SDS/PAGE) and subsequent western

31 Analyses by SEC, blue native PAGE, SDS-PAGE, and dynamic light scattering indi

32 sis of CI in Ndufs4(fky/fky) mice using blue native PAGE revealed the presence of a faster migrating

33 erization under native conditions using blue native PAGE.

34 probe IRE1alpha complexes in cells with blue native PAGE immunoblotting.

35 Blue native-PAGE analysis revealed defects in the organizatio

36 Western blot, immunoprecipitation, and Blue native-PAGE analysis.

37 e with ATR after cisplatin treatment by blue native-PAGE and co-immunoprecipitation.

38 ng of SCAP to INSIG-1, as determined by blue native-PAGE, and this is correlated with the inhibition

39 were observed in separate complexes by blue native-PAGE, the two proteins coimmunoprecipitated both

40 Following incubation at 47 degrees C, blue native-PAGE revealed a lower oligomeric form (alpha2beta

41 ted a large molecular weight complex in blue native-PAGE (~1.0 MDa), corresponding to the total molec

42 5 showed distinct migration patterns on blue native-PAGE.

43 ed into multiprotein complexes, we used blue native-PAGE (BN-PAGE) and cross-linking techniques to id

44 Blue-native PAGE analyses of intact oligomers and disulfide c

45 Blue-native PAGE analysis showed that the variant TatC protei

46 SRs with unprocessed 12S globulin by 2D blue-native PAGE/SDS-PAGE indicated that the nhx5 nhx6 knocko

47 ar vesicles and migrated as a trimer by blue-native PAGE.

48 , focusing on mammalian synapses we use blue-native PAGE and 'gene-tagging' of GluN1 to report the fi

49 ccurs when tafazzin is missing, we used blue-native PAGE and gene expression analysis to determine th

50 ometry (DIP-MS), which combines AP with blue-native-PAGE separation, data-independent acquisition wit

51 By native PAGE, formation of S-nitrosylated SP-D in vivo re

52 By native PAGE, it was possible to detect formation of a co

53 is moderate with high fidelity, confirmed by native PAGE, thermal transition study, and Ferguson anal

54 R are able to form a 1:1 complex detected by native PAGE.

55 nd single-stranded DNA, but as determined by native PAGE analysis, the protein cannot form a stable c

56 with (59)Fe-labeled transferrin followed by native PAGE electrophoresis.

57 ilibrium between these forms is indicated by native PAGE, which also reveals additional single-strand

58 tion of thylakoid complexes was performed by native PAGE and sucrose density centrifugation.

59 f the mass of the tRNA fragments resolved by native PAGE.

60 ic E5 channel stoichiometry was suggested by native PAGE studies.

61 rface was further validated both in vitro by native PAGE and in cellulo by cell-clustering and dendri

62 and analysis of the translation products by native-PAGE was used to study the maturation pathway of

63 monomeric by size exclusion chromatography, native PAGE, and small angle x-ray scattering.

64 Analytical ultracentrifugation and clear native PAGE analysis show that NRC is a stable pigment-p

65 from the membrane, are studied for coupling native PAGE with MALDI-MS.

66 E showed lower-than-expected mobility during native-PAGE, the largest hydrodynamic radius for the mon

67 naturing polyacrylamide gel electrophoresis (native PAGE) to have substantial decreases in their Mg2+

68 Studies employing native PAGE suggest that most nDNA-encoded CI subunits f

69 Gel filtration, native PAGE, and protein cross-linking were used to anal

70 However, native PAGE in gels containing amylopectin revealed that

71 Immunoprecipitation, native PAGE, and functional studies confirmed that the G

72 ich comigrated with the protease activity in native PAGE and yielded multiple bands in SDS-PAGE under

73 0-kDa MP, which migrated as a single band in native PAGE.

74 S-PAGE showed that each of the bands seen in native PAGE was composed of several polypeptides.

75 emical and biophysical techniques, including native-PAGE, binding affinity by fluorescence anisotropy

76 Off-line combination of native PAGE with MALDI-MS is demonstrated for high-resol

77 enhanced chemiluminescence visualization of native PAGE gels and through acrylamide fluorescence que

78 x had a total mass of 77 kDa and migrated on native PAGE at 75 kDa.

79 t to stabilize higher-order Bak oligomers on native PAGE, suggesting an important role in the Bak oli

80 n in P. aeruginosa cell lysates separated on native PAGE gels and stained for iron with Ferene S.

81 ws CA activity and is distinct from TWCA1 on native PAGE of radiolabeled T. weissflogii cell lysates.

82 igrated at their monomer molecular weight on native PAGE.

83 A single prominent protein band appeared on native-PAGE and SDS-PAGE implying that the mPPO is a mon

84 urified trypsin appeared as a single band on native-PAGE.

85 wever, upon anion-exchange chromatography or native PAGE, CT separates from the BC and BCCP subunits

86 nge chromatography and analyzed by SDS-PAGE, native PAGE, and Western immunoblot analysis.

87 Parallel native PAGE, immunoblotting and Complex I activity assay

88 r dichroism spectroscopy, thermal stability, native PAGE, and analytical ultracentrifugation indicate

89 These results highlight the power of the native PAGE assay for membrane protein biochemistry and

90 ch adopt multiple conformations according to native PAGE.

91 mutagenesis, analytical ultracentrifugation, native PAGE, and activity assays, we found that these co

92 enzyme using denaturing SDS-PAGE but, under native PAGE conditions, was not observed to form the app

93 We used native PAGE to follow the activity of Cif from the human

94 of the ZPI-binding interface, together with native PAGE and kinetic analyses of PZ binding to ZPI, t