ライフサイエンスコーパス: native protein

1 tivity different from that of the metal in a native protein.

2 mic mRNAs, in the presence or absence of the native protein.

3 effective as antibodies directed against the native protein.

4 self-antigen, rather than a response to the native protein.

5 experiments on the same mutant could produce native protein.

6 tepwise pathway that sequentially builds the native protein.

7 nism involves extensive clearance of the non-native protein.

8 native-like structural elements to build the native protein.

9 d more dichlorogenic acids compared with the native protein.

10 mprove the thermostability of a well-behaved native protein.

11 has a quaternary structure distinct from the native protein.

12 lism was nevertheless more extensive for the native protein.

13 de and deleting the different domains of the native protein.

14 trans form of the surfactant relative to the native protein.

15 +/- 0.004) of E181Q relative to that of the native protein.

16 and induces antibodies against the unaltered native protein.

17 ng and enhances the overall stability of the native protein.

18 esidue-resolved solvent accessibility of the native protein.

19 DnaB is depleted at the oriC relative to the native protein.

20 mpled through stochastic fluctuations of the native protein.

21 iciting antibodies that cross-react with the native protein.

22 ence polypeptide also approaches that of the native protein.

23 e primase activity more efficiently than the native protein.

24 apoptotic cells at a level comparable to the native protein.

25 s for endogenous esterases, which regenerate native protein.

26 of unknown function that is cleaved from the native protein.

27 the rapid misfolding and aggregation of the native protein.

28 s, they are extremely difficult to purify as native protein.

29 reby maintaining the cellular pool of active native proteins.

30 s protease-degradable or biologically active native proteins.

31 use ATP to dissociate and convert them into native proteins.

32 ein research, in particular for the study of native proteins.

33 p-regulating ERAD components that remove non-native proteins.

34 ns of cysteines or lysines, respectively, in native proteins.

35 ropagating aggregation of nominally healthy, native proteins.

36 purification yield and authentic folding for native proteins.

37 P and convert stable protein aggregates into native proteins.

38 eins and conferring increased stability upon native proteins.

39 d heme c proteins as reliable models for the native proteins.

40 ized protein oligomers generated from common native proteins.

41 dependence of local protein unfolding within native proteins.

42 n and subsequent glucose release compared to native proteins.

43 ique method to enhance the immunogenicity of native proteins.

44 der physiologically relevant conditions with native proteins.

45 rts different chemical reactivities than the native proteins.

46 teric response comparable to the activity of native proteins.

47 romatographic quantification of the residual native proteins.

48 ities that open and close to encapsulate non-native proteins.

49 nanoreactors for single-molecule studies of native proteins.

50 purified recombinant proteins in adjuvant as native proteins.

51 ydrophobic segments across the length of non-native proteins.

52 morphic residues, which significantly affect native protein activity, structure, or function and are

53 -related and neurological diseases, formerly native proteins aggregate via formation of a partially u

54 scape, which results in the formation of non-native protein aggregates that challenge the capacity of

55 rthermore, we demonstrate that for validated native protein aggregates, sorting to the cellular pole

56 To further investigate the role of HspX in native protein aggregation, we performed semi-quantitati

57 tm1-Pdi1 complex processes both unfolded and native proteins albeit with a preference for the former.

58 This approach adopts native proteins (albumin) as bio-receptors that interact

59 essed at substantially lower levels than the native protein, allowing assessment not only of the lack

60 Degradation of specific native proteins allows bacteria to rapidly adapt to chan

61 Applications ranging from native protein analyses to molecular library constructio

62 duce inaccurate concentration assignments of native protein analytes by bottom-up analysis due to var

63 ng molecular dynamic simulations of both the native protein and a realistic denatured state ensemble.

64 is antigenically indistinguishable from the native protein and forms a homogeneous trimer in solutio

65 specially viscera, as a source for obtaining native protein and hydrolysates, explaining their produc

66 omain, induces antibodies that recognize the native protein and interfere with S. mutans adhesion in

67 nd to exhibit biophysical characteristics of native protein and reacted with native-OVA specific mono

68 d into Escherichia coli and expressed as the native protein and with an N-terminal His(6) tag.

69 detailed protocols for the immunization with native proteins and for the selection by phage display o

70 o-clustering with other existing clusters of native proteins and lipids in the membrane.

71 ptides show higher antioxidant capacity than native proteins and may be absorbed in the intestine wit

72 aperone-mediated kinetic partitioning of non-native proteins and may help explain the etiology of sep

73 pair facilitates the study of RNA binding to native proteins and peptides, which is demonstrated here

74 release agent able to produce nonaggregated native proteins and preserve the overall nature of the m

75 pillaries for nanoelectrospray ionization of native proteins and protein complexes effectively reduce

76 d pairs in close proximity on the surface of native proteins and protein complexes.

77 potential valuable tools for the MS study of native proteins and protein complexes.

78 For native proteins and their complexes, many structural fea

79 ergy of ATP binding and hydrolysis to unfold native proteins and translocate unfolded polypeptides in

80 [(32)P]c-di-GMP bound several E. chaffeensis native proteins and two E. chaffeensis recombinant I-sit

81 truggle to separate conjugates from polymer, native protein, and from isomers has vexed scientists fo

82 nock-in are regulated and expressed like the native protein, and in the heterozygous condition mediat

83 nfolded protein are slower than those of the native protein, and the unfolded protein fast dynamics a

84 periments show that the fast dynamics of the native protein are virtually temperature independent.

85 Conformational change and aggregation of native proteins are associated with many serious age-rel

86 Such studies have found that native proteins are brittle, and thus not very deformabl

87 nd to be unrealistic because the dynamics of native proteins are dominated by subglobal transitions a

88 Many native proteins are multi-specific and interact with num

89 Active sites and ligand-binding cavities in native proteins are often formed by curved beta sheets,

90 an form aggregate-like structures from which native proteins are restored by ATP-dependent chaperones

91 -terminal regions (approximately half of the native protein) are phenotypically near-normal; and thos

92 at commonly used haptenated proteins, unlike native proteins, are inherently immunogenic.

93 ntal strategy for global characterization of native protein assemblies based on chromatographic fract

94 -down proteomics experiments, especially for native protein assemblies.

95 mmalian expression system where the rules of native protein assembly are strictly obeyed.

96 SP, SCATD and SCWRL4) on both native and non-native protein backbones.

97 s are, however, distinct from those with the native protein because the metal complex occupies the su

98 Thus, helices, in contrast with native proteins, become more stable with increasing pres

99 rol (QC) activity in the partitioning of non-native proteins between life and death are not clear.

100 folded fragments, but it is not clear if the native protein binds fibronectin with the same primary s

101 d terminal cysteinate were suggested for the native protein but have not yet been confirmed experimen

102 n signal are fully exposed and mobile in the native protein but partially sequestered in the MA(NOS)

103 In conclusion, the native proteins (but not the nucleic acids, lipids or ca

104 Mcl1 is present at levels comparable to the native protein, but it is markedly stabilized in cells s

105 trates in the first solvation shell of quasi-native proteins, but with a density lower than that of t

106 agged PSBS that were exploited to purify the native protein by affinity chromatography.

107 ism by which Hsp90 can recognize and remodel native proteins by binding and remodeling partially fold

108 Relief from excess levels of non-native proteins by increasing the levels of folding fact

109 ction was confirmed by GST pulldown and, for native proteins, by co-immunoprecipitation experiments i

110 argeted proteomics rationale to the level of native protein complex analysis (complex-centric proteom

111 ated with a smaller protein complex than the native protein complex associated with endogenous SHB1.

112 a slightly smaller protein complex than the native protein complex, but it largely displaced endogen

113 t largely displaced endogenous SHB1 from its native protein complex.

114 MPC-iron complex than native protein complex.

115 stoichiometry and composition of endogenous native protein complexes at an unprecedented level of de

116 approach allows the biochemical isolation of native protein complexes for proteomic studies.

117 gmentation approach, or (pseudo)-MS(3), from native protein complexes to a set of constituent fragmen

118 hogonal handles for biochemical isolation of native protein complexes.Split fluorescent proteins (FPs

119 Mass spectrometry analysis of the native protein confirmed the post-translational modifica

120 art" biomaterial scaffold, which retains the native protein conformation and tensile strength of the

121 r warfarin sensitivity is to stabilize their native protein conformation in vitro.

122 We also show that native protein conformation is conserved in TENG-ESI, an

123 tein microarray such as covalent attachment, native protein conformation, homogeneity of the protein

124 a unique capacity to bind and stabilize non-native protein conformations, prevent aggregation, and k

125 Non-native protein conformers generated by mutation or chemi

126 he hypothesis explored in this work was that native proteins could be digested and identified without

127 scription factors (NANOG, MYOD), antibodies, native proteins (cytochrome C), magnetic nanoparticles (

128 Starting from the native Protein Data Bank structure, nine (meta)stable st

129 ut their kinetic profiles are different; the native protein did not show any such tendency under the

130 dditional structures were determined for the native protein (dmin = 2.0 A) as well as binary complexe

131 nding affinity prediction, discrimination of native protein-DNA complex from decoy structures, and mo

132 kinase are not affected by loop elongation, native protein dynamics that are essential for efficient

133 of intramolecular signaling and catalysis to native protein dynamics that arise from modest changes i

134 ial for using stably expressed tFTs to study native protein dynamics with high temporal resolution in

135 ibrils was measured in vitro and compared to native protein, early-stage-fibrillar protein, and sonic

136 ous insert were positioned in frame with the native protein-encoding sequences but were separated fro

137 chanical disruption of a metal center in its native protein environment in aqueous solution.

138 hese chemically identical molecules in their native protein environment.

139 Gjd3 regulatory elements without perturbing native protein expression.

140 simple, fast, robust, cheap and adequate for native protein extraction, being a potential approach fo

141 recapitulates the self-assembly mechanism of native protein fibrils in which a ligand binding event g

142 eating sites for amyloid growth and, as with native protein folding, appear important for backbone de

143 appropriate micellar environment to preserve native protein folding.

144 antagonize prion formation by protecting the native protein from misfolding or by targeting it for de

145 hogenesis is mediated by misappropriation of native protein function, a mechanism that may apply broa

146 The non-native protein functions also raise the prospect of futu

147 ne)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sul

148 The promiscuous interaction with non-native proteins generates complexes that can form aggreg

149 CHIP-mediated ubiquitination does not affect native proteins has important pharmacological implicatio

150 tte of reaction chemistries in re-engineered native proteins has proved challenging.

151 etween modular domains within the context of native proteins have been largely unexplored.

152 bstitutions rarely increase the stability of native proteins; hence, large libraries and high-through

153 c peptides are built by assembling 7-residue native-protein heptad modules into new combinations.

154 screening the quaternary organization of 60 native proteins identified numerous discrete supercomple

155 ppear to work through the same mechanisms as native protein immunotherapy.

156 tions among different influential factors in native proteins impede progress toward complete understa

157 he small number of representative pockets in native proteins implies that promiscuous interactions ar

158 ng ALA protein was less immunogenic than the native protein in rabbits.

159 uent uptake of fibrils that directly contact native protein in recipient cells.

160 t acted as a dominant negative retaining the native protein in the endoplasmic reticulum.

161 surface versus the dynamics displayed by the native protein in the hydrated solid state.

162 ficiently analyzes proteolytic fragments and native proteins in a complex mixture.

163 yte-filled nanopores can characterize single native proteins in an aqueous environment, but currently

164 sHsps complex with a variety of non-native proteins in an ATP-independent manner and, in the

165 dily usable protocol for the transduction of native proteins in C. elegans, which is based on the enc

166 enabled the detection of UAG readthrough in native proteins in E. coli strains in which UAG was reas

167 Model peptides in buffer as well as native proteins in human blood plasma additionally exhib

168 stly been studied using stably expressed non-native proteins in immortalized cell lines.

169 rify the interactions amongst the endogenous native proteins in live cells under native resting condi

170 rapid efficient photodeprotection to reveal native proteins in live cells.

171 ion on the thermal stability of thousands of native proteins in live cells.

172 Molecular chaperones act on non-native proteins in the cell to prevent their aggregation

173 xM method did not result in the retention of native proteins in the gel and relied on custom-made rea

174 se machine that rescues various forms of non-native proteins including the highly resistant amyloid f

175 nd binding assays with peptides derived from native proteins indicated that these two PDZ domains hav

176 re of oligomers and fibrils sequestrates the native protein into an inactive conformation that is typ

177 can induce the conformational conversion of native proteins into aggregates that can be transmitted

178 to simply purify mixtures of conjugates and native proteins into single species.

179 ss spectrometry (IM-MS) allows separation of native protein ions into "conformational families".

180 out" experiments, where the concentration of native protein is compared both with and without the pre

181 sured for pure lysozyme, suggesting that the native protein is dormant on the nanosecond time and nan

182 ody against recombinant Bgl2 showed that the native protein is localized primarily to the trophic for

183 osphorylated FadD32 isoforms showed that the native protein is phosphorylated by serine/threonine pro

184 ions and post-translational modifications of native proteins is a challenge for research and diagnost

185 mation between aldehydes and amino groups in native proteins is markedly disfavored due to protonatio

186 des or other linear substrates compared with native proteins is most likely related to the reduced ac

187 ely prepare stable OACs from amino groups on native proteins is presented.

188 The oxidatively damaged, but not the native protein, is a substrate of the Pln protease.

189 rotein isolates was lower than the gels from native protein isolates and gel strength increased with

190 re observed in treated protein isolates than native protein isolates and increased with increase in t

191 as observed in treated protein isolates than native protein isolates and was further reduced with inc

192 resistance against thermal degradation than native protein isolates as was evident from thermal grav

193 ts may be compromised by the presence of non-native protein junctions that result in protein misfoldi

194 ant protein with sequence identical with the native protein lacking the N-terminal pyroglutamate (the

195 quantify post-translational modifications at native protein level in order to correlate their influen

196 to characterize the sequences of proteins in native protein-ligand and protein-protein complexes and

197 ID), for the top-down MS characterization of native protein-ligand and protein-protein complexes.

198 ress the effect of ion source temperature on native protein-ligand complex ions as assessed by SID.

199 Benchmark tests on 8 native protein-ligand complexes show that the method can

200 are also successfully modelled, reproducing native protein-ligand contacts with significantly differ

201 optimize the quantified specificity of the "native" protein-ligand complex discriminating against "n

202 ingle and mixed polymers, and self-assembled native protein-lipid complexes (Nanodiscs).

203 we investigate how the reverse-sequences of native proteins might fold by examining a series of smal

204 The interaction of Hsp40s with native proteins modifies their structure and function.

205 Native proteins often lack immunogenicity and thus limit

206 of DMSO can reduce in-source dissociation of native protein oligomers and their interactions with hyd

207 s approach can be used to make inhibitors of native proteins, or to develop novel peptides for applic

208 ability of c-Jun to heterodimerize with its native protein partner, c-Fos, and therefore designed a

209 acokinetics and therapeutic effects over the native protein/peptide upon administration to mice.

210 itous chaperones that bind and sequester non-native proteins preventing their aggregation.

211 Besides inducing misfolding of native proteins, prions bind nucleic acids and other pol

212 divergence was observed in the reporter and native protein profiles depending on the difference in t

213 Native protein-protein binding was confirmed by co-immun

214 icity which determines the discrimination of native protein-protein complex against competitive ones.

215 ation of this strategy to a more challenging native protein-protein interaction (PPI) suggested that

216 Top-down MS of the native protein provides complementary sequence informati

217 valuating the extent of glycosylation of the native proteins, providing valuable information when the

218 (1) the primary reference material BCR457-a native protein purified from human thyroids, (2) a comme

219 classification scheme for identification of native protein quaternary assemblies (binders/nonbinders

220 ls who lacked specific IgE when tested using native protein reagents.

221 d in the recognition of dimethyl arginine by native protein receptors.

222 tide substrates from one another, leading to native protein refolding.

223 Site-selective bioconjugation to native protein residues is a powerful tool for protein f

224 Electrospray ionization (ESI) of native proteins results in a narrow distribution of low

225 RBDmap thus yields profound insights into native protein-RNA interactions in living cells.

226 that the beta-barrel is responsible for the native protein's amphiphilicity.

227 ide strategy, we designed a peptide with non-native protein sequence, AP3, which exhibited potent ant

228 ave not been performed in the context of the native protein sequence.

229 tackle these questions, we in silico mutated native protein sequences into random sequence-like ensem

230 nexplored, one might speculate that reversed native protein sequences should be significantly more fo

231 s revealed important differences between the native protein shell and the empty capsid.

232 the degree of denaturation, compared to the native protein solution, was observed.

233 oncentrations of potentially aggregating non-native protein species within the cell.

234 es indicates that the characteristics of the native protein structural fluctuations that cause proton

235 s been made in designing proteins that mimic native proteins structurally, it is more difficult to de

236 s the potential for simultaneous analysis of native protein structure and spatial distribution within

237 where Zn(2+) is crucial for maintaining the native protein structure but the Zn-bound cysteines are

238 Computationally generated non-native protein structure conformations (or decoys) are o

239 Access to native protein structure depends on precise polypeptide

240 Voids and cavities in the native protein structure determine the pressure unfoldin

241 ectrostatic separation process preserves the native protein structure found in NBF and improves the r

242 or these cationic residues in the context of native protein structure where histidine protonation sat

243 ein folding, unlike the formation of stable, native protein structure, does not present a substantial

244 ely to lead to artifactual conclusions about native protein structure.

245 the three modifications, would be buried in native protein structure.

246 protein folding pathways leading to knotted native protein structures are slower and less efficient

247 n blind test of a computer method to predict native protein structures based solely on an all-atom ph

248 knowledge-based potentials in selecting the native protein structures from decoy sets.

249 Entropic stabilization of native protein structures typically relies on strategies

250 kbone hydrogen bonds are a common feature of native protein structures, yet their thermodynamic and k

251 ains a major challenge in creating mimics of native protein structures.

252 made it especially suitable for the study of native protein structures.

253 produced are often more compact and reflect native protein subcomplexes when compared with unmodifie

254 HslU hexamers recognize and unfold native protein substrates and then translocate the polyp

255 e ATP-dependent unfolding and degradation of native protein substrates in conjunction with ClpP1P2, b

256 emonstrate that although AtPARK13 can act on native protein substrates, unfolded proteins represent b

257 ea, which are known to stabilize or denature native proteins, suggesting that interface-driven Abeta

258 n common with unfractioned SEA, the purified native protein suppresses lipopolysaccharide-induced DC

259 Designing proteins or peptides that bind native protein targets can aid the development of novel

260 ing of a secondary structural element of the native protein, termed a "foldon." Each folded segment i

261 By connecting the native protein termini via a covalent linker and introdu

262 which displays the defining features of the native protein that have not been attainable from struct

263 Living systems have chiral molecules, e.g., native proteins that almost entirely contain L-amino aci

264 ess, these tools are based on engineered non-native proteins that may (i) express at nonphysiological

265 es result in distinct binding modes with non-native proteins that ultimately define the activity of t

266 As a programmable tool orthogonal to any native proteins, the DNA-lipid tethers can be further ap

267 Unfortunately, the native proteins themselves are often unstable in physiol

268 both termini, suggest their proximity in the native protein; these are also disordered, based on POND

269 Addition of native protein to the noodles had less impact on degree

270 lenges associated with the administration of native proteins to C. elegans have limited the range of

271 that LSs can be chemically cleaned to remove native proteins to create intact clean hollow LS shells.

272 eta-sheet-rich structures in transition from native proteins to ordered oligomers and fibres.

273 released to a cf-IMER column which converted native proteins to peptides in 5 min at elevated tempera

274 ion could provide a general means to deliver native proteins to the cytosol.

275 haperone Hsc70 assists in the folding of non-native proteins together with its J domain- and BAG doma

276 ated protein treated mice as compared to its native protein treatment.

277 raffic to the plasma membrane, and place the native protein under optical control provided by the coa

278 -based AFM to the multiparametric imaging of native proteins under physiological conditions.

279 During amyloid formation, the native protein undergoes a tetramer-to-folded monomer tr

280 here a scheme for optical remote control of native proteins using a "photoswitchable conditional sub

281 igh-sensitivity, high-resolution analyses of native protein variants.

282 l electrophoresis enabled rapid screening of native protein variants.

283 modest resolution (2.8 to 2.3 angstroms) for native proteins varying in size (127 to 1148 unique resi

284 The molecular weight of the native protein was estimated at approximately 59,400, a

285 5171 specific antibodies and showed that the native protein was expressed by the three life cycle sta

286 n proteins to complement the function of the native proteins was demonstrated by nutritional and salt

287 fied recombinant MATN-1, along with purified native protein, was shown to inhibit angiogenesis in viv

288 sidues within the inter-domain linker in the native protein, we were able to show that despite the re

289 18 mutants of the BLM protein along with the native protein were further investigated using molecular

290 cellular uptake (as compared to that of the native protein) were achieved.

291 tart of gel photopatterning to completion of native protein western blotting, a substantial time savi

292 For both recombinant and native proteins, western analysis detected MERTK interac

293 tant in the MBP sequence but adjacent in the native protein where they close the longest residue-to-r

294 t AML-1b showed the same band pattern as the native protein, whereas recombinant AML-1a in the reduce

295 hanced neuronal expression compared with the native protein while maintaining transcriptional activat

296 (DMG) intermediates, an expanded form of the native protein with a dry core, have been observed durin

297 al route to generating synthetic variants of native proteins with more selective binding profiles.

298 l signal peptides mediate the interaction of native proteins with the translocon complex of the endop

299 n degradation machinery to remove unmodified native proteins within minutes of application.

300 A molecule page starts with astate of a native protein, without any modification and/or interact