ライフサイエンスコーパス: native state

1 progressively funneling the molecule to the native state.

2 that the intermediate strongly resembles the native state.

3 ions, designed to reduce the dynamics of its native state.

4 ide the context for further folding into the native state.

5 y MG and forces the nascent chain toward the native state.

6 f freedom and the overall flexibility of the native state.

7 gements, making it difficult to study in its native state.

8 ions with chaperones before folding into its native state.

9 bel-free, biomolecular fingerprinting in the native state.

10 the protein along productive pathways to the native state.

11 cs rather than binding preferentially to the native state.

12 t able to support part of the search for the native state.

13 ap prevents the intron RNA from assuming its native state.

14 adopted as an alternative to the functional, native state.

15 the entire energy landscape and not just the native state.

16 echnique for analyzing biomolecules in their native state.

17 as able to significantly protect the protein native state.

18 protein into a topology close to that of its native state.

19 nsions of an intact vertebrate in a close-to-native state.

20 olumn chemistries without compromising their native state.

21 ormations, slowing productive folding to the native state.

22 preformed amyloidogenic beta-strands in the native state.

23 characterize the P13 protein complex in its native state.

24 e the cell over the elongated aqueous buffer native state.

25 tructure and active-site cleft formed in the native state.

26 ong different parallel tracks leading to the native state.

27 mature Pseudomonas aeruginosa biofilm in its native state.

28 setting, with high precision, and in a near-native state.

29 hich leads to the spontaneous folding of the native state.

30 le proteins are strongly funneled toward the native state.

31 ps to maintain proteins in their functional, native state.

32 of the population for 1.4 ms en route to the native state.

33 slower 1.4-ms phase during refolding to the native state.

34 ce, and location of ultrastructure in a near-native state.

35 unique three-dimensional structure in their native state.

36 lecular conformations that exchange with the native state.

37 IkappaBalpha AR domain was unfolding in its native state.

38 t adopt a stable tertiary structure in their native state.

39 in directing the folding process toward the native state.

40 ise consolidation that ultimately yields the native state.

41 no known propensity for instability in their native state.

42 s that correspond to the four helices in the native state.

43 ion to focus on regulation of genes in their native state.

44 r, ensuring the efficient formation of their native state.

45 mes do not lose activity compared with their native state.

46 hanged to its NIS-bound state instead of the native state.

47 ective lens to image frozen samples in their native state.

48 regates and assist in their refolding to the native state.

49 es for each to propagate to the final folded native state.

50 is a useful tool to study proteins in their native state.

51 olded state and PP2 helical structure in the native state.

52 guide it along an ordered pathway toward the native state.

53 ation that is topologically simpler than the native state.

54 energy landscape and fold efficiently to the native state.

55 ding energy landscape more biased toward the native state.

56 ures of macromolecular complexes in close to native states.

57 be an important tool for describing protein native states.

58 parallel pathways between the denatured and native states.

59 d in the hydrophobic cores of the respective native states.

60 solution structures of the proteins in their native states.

61 ated almost halfway between the unfolded and native states.

62 ions that occur in unfolded RNAs relative to native states.

63 at are most sensitive to the presence of non-native states.

64 is of unperturbed cells and tissues in their native states.

65 -atom simulations for both the denatured and native states.

66 y important biological systems in their most native states.

67 morphological features and changes from the native states.

68 gical samples of complex topography in their native states.

69 ue dissociation and preserves transcripts in native states.

70 In low light, the OCP relaxes to the native state, a process that is accelerated in the prese

71 energetic conflicts are minimized in folded native states according to the principle of minimal frus

72 e use Markov models to determine whether the native state acts as a facilitator for the transition, o

73 4 of the 12 molecules, we conclude that the native state acts primarily as a trap, which is consiste

74 = 250 ns, the fraction of structures in the native-state (alpha-helical) basin equals 22%, which is

75 In the meantime, the native state also becomes more unstable and the amyloid

76 ants are rapidly committed to folding to the native state, although they reach the native state with

77 formed in 6 pigs; 5 studies were done in the native state and 4 after infarction of the left anterior

78 nges between a highly populated, NMR-visible native state and a conformationally excited, NMR-invisib

79 the overall interconversion rate between the native state and a well-defined folding intermediate by

80 field-free analysis of biomolecules in their native state and at or near physiological salt concentra

81 zation of subcellular components in the near-native state and at sub-molecular resolutions in single

82 of the target protein while maintaining its native state and biological activity.

83 e for the full-length glycoprotein in a near-native state and compared it to that of a glycoprotein l

84 ts unique ability for imaging cells in their native state and determining structures of macromolecula

85 The correlation between energy of the native state and experimental denaturation temperature w

86 sualization of cellular organization at near-native state and in sub-molecular resolution, making it

87 bed in terms of transitions between a folded native state and less-populated intermediates, or excite

88 how that the denatured RfaH refolds into the native state and that RfaH in which the order of the dom

89 the ratio of the free energy gap between the native state and the average of non-native states versus

90 able SP, we show that as the SP folds to the native state and the population of unfolded SP declines,

91 t is, the free energy difference between the native state and the unfolding transition state-dictates

92 rs (serpins), typically fold to a metastable native state and undergo a major conformational change i

93 operative two-state transition involving the native state and unstructured denatured species.

94 ner, i.e. by lowering the free energy of the native state and with almost no effect on the free energ

95 entails attractive forces for the functional native states and discrimination against potential nonna

96 h atomic resolution 3D structures of protein native states and some folding intermediates are availab

97 commercial gasoline mixtures, both in their native states and spiked with different adulterants.

98 ages of the macroautophagy pathway in a near-native state, and 3) reveal that vaults arrive early in

99 onal domains to the monomer destabilizes the native state, and the entropically stabilized amyloid-li

100 are investigated both as-prepared, in their native state, and under reaction conditions, while trans

101 en the metastable mirror-image state and the native state; and (iv) offer a plausible reason to expla

102 In native states, animal cells of many types are supported

103 tration, wherein not all interactions in the native state are optimized energetically, thereby potent

104 transition states from both I1 and I2 to the native state are plastic and change with mutation and de

105 ing progression from the intermediate to the native state are those which define the native binding i

106 ely in an all-or-none fashion and thus their native states are well protected from thermal fluctuatio

107 foreshadowed by segmental flexibility in the native state as probed by heteronuclear NMR spectroscopy

108 cting the protein folding into a compact non-native state, as well as protein interactions with the l

109 ES is required by a set of proteins to reach native state at a biologically relevant timescale, avoid

110 three-dimensional images of cells in a near-native state at macromolecular resolution, but identifyi

111 al and computational methods to describe non-native states at atomic resolution.

112 and suggests that the general concept of the native state be expanded to allow for more variable phys

113 at both proteins exist as tetramers in their native state before and after proteolysis.

114 ed directly from cells shows mLST8 in a near-native state bound to CCT deep within the folding chambe

115 eas the latter can capture proteins in their native state but has a low signal-to-noise ratio and yie

116 Thus, P3 is disrupted during folding to the native state but not to the misfolded state, and P3 disr

117 rd, promoting normal skin homeostasis in the native state, but can be metabolized to sphingosine-1-ph

118 e energetically disfavored compared with the native state, but entropically favored.

119 same glycans can be identified also in their native state by CE-MS without derivatization.

120 However, guiding the sampling towards the native state by effectively using restraints remains a m

121 ging of biological materials, preserved in a native state, by using an OrbiSIMS instrument equipped w

122 hods to probe the adhesion of cells in their native state can greatly advance the understanding of co

123 , discuss how local backbone disorder in the native-state can lead to non-classical Phi(M)-values (Ph

124 on of the Xi topological arrangement in near-native state cells.

125 beam milling, allowing the reconstruction of native-state cellular sections at molecular resolution.

126 ed state of HEWL possesses a large degree of native state characteristics.

127 As were long, highly m(6)A modified in their native state, characterized by low folding energies, and

128 c dynamics, encoded by their structure under native state conditions.

129 We propose that the native state contains structural elements that frustrate

130 cells under two different conditions, i.e., native state (control) and latrunculin B (treated), we s

131 we demonstrate that stability of the protein native state determines both protein aggregation propens

132 nalysis of the xanthophyll fraction in their native state (direct extract) revealed that most of them

133 e productive structural rearrangement to the native state does not occur in a single swift step but i

134 Steric clashes in the native state due to the new side chain are rarely observ

135 ns is an important factor in determining the native-state dynamical properties of human lysozyme in t

136 The results indicate the importance of native state dynamics in initiating the aggregation of p

137 plete picture of the energy landscape of the native state dynamics of an AR domain.

138 olecular dynamics simulations to compare the native state dynamics of Beta-2 microglobulin (beta2m),

139 the loop as well as its size influences the native state dynamics, which can impact receptor binding

140 alternative approach to protein folding and native-state dynamics presents a generally applicable st

141 ddress the effects that location may have on native-state dynamics, as studied by hydrogen-deuterium

142 utation (I23A versus I59T) is crucial to the native-state dynamics, with the alpha-domain mutation ha

143 One mutation destabilizes native-state electrostatic interactions but has a larger

144 uld be attributed to fluctuations within the native state ensemble.

145 ated from within a thermodynamically stable, native-state ensemble.

146 This remarkable native-state expansion can be explained quantitatively b

147 ly quenched by surrounding nitroxides in the native state; exposure to ascorbate or ascorbate/glutath

148 ather than for the overall unfolded-state to native-state folding pathway.

149 y gated ET, which involves conversion to the native state followed by reduction, with a small fractio

150 assembly of new proteins in their functional native states following biosynthesis on the ribosome.

151 The dominance of a single native state for most proteins under ambient conditions

152 action and of the ability to protect the TTR native state for polyphenols, both stilbenoids and flavo

153 tion of the scaffolded assemblies highlight "native-state" forced protein-protein interfaces, which m

154 We show that both hGAAP and vGAAP in their native states form oligomers and that oligomerization is

155 iminate unassembled proteins en route to the native state from misfolded ones that need to be degrade

156 maging technique of vitreous samples in near-native state has matured over the past two decades and p

157 cdB at pH 4 and 7 suggests that the pH 4 and native state have similar but nonidentical structures.

158 However, the protein has an unusually high native-state heat capacity, and consequently, the change

159 ence of (1)HN and (15)N chemical shifts, and native-state hydrogen exchange at urea concentrations wh

160 Moreover, native-state hydrogen exchange indicates that Hsp90 can

161 The native-state hydrogen-exchange data suggest that the six

162 regions of proteins and in proteins in a non-native state (i.e. misfolded or unfolded states).

163 t is essential to keep the cells under their native states (i.e. label-free), establishing plasmonic

164 tions, this intermediate either folds to the native state in a few minutes, or forms amyloid fibers i

165 ative structural description of LDs in their native state in HeLa cells enabled by cellular cryoelect

166 released intermediates to slowly reach their native state in solution.

167 nformational dynamics of beta2-microglobulin native state in the crystal lattice is in keeping with w

168 imum WUE(e) across all biomes; and a minimum native state in wetter years that was common across hydr

169 responding to the denatured, transition, and native states in the folding of the activation domain of

170 chemical analysis on target samples in their native states in the undisturbed environment.

171 , whereas the third introduces unanticipated native-state interactions but does not alter electrostat

172 - or three-state mechanisms involving mainly native-state interactions, yet recent network models bui

173 ons in gpW at 1 degrees C, we found that the native state interconverts with a transiently formed, sp

174 DESI enabled the direct, rapid, native state interrogation of oil samples on steel surfa

175 n of peptides or proteins from their soluble native states into intractable amyloid deposits is assoc

176 hod might be applicable to proteins in their native state, irrespective of location, concentration, a

177 age of trajectories that are mediated by the native state is 31% when averaged over all molecules, an

178 e native fold; the low fold stability of the native state is a hallmark of aggregation propensity.

179 he transition from the unfolded state to the native state is entropically disfavored at 298 K.

180 that restoring degraded ecosystems to their native state is generally superior to agricultural produ

181 cytoskeletal functions in the cytoskeleton's native state is inherently difficult due to its rigid an

182 gically-reasonable temperatures at which the native state is marginally stable.

183 under thermodynamic conditions in which the native state is no longer stable, such as denaturing sol

184 ve and repulsive exosite interactions in the native state is shifted to favor the attractive interact

185 Mass analysis of the intact protein in its native state is straightforward and fast, requires very

186 of this species is often difficult since the native state is usually predominantly populated at equil

187 HP24stab shift the energy balance toward the native state, leading to a minimal folding motif.

188 the orthogonal fragment screening methods of native state mass spectrometry and surface plasmon reson

189 t there is a tremendous opportunity to apply native state mass spectrometry as a complementary fragme

190 an underutilized fragment screening method, native state mass spectrometry, together with two proven

191 coupled to multi-angle light scattering and native-state mass spectrometry of Yorkie WW domains inte

192 known whether extracellular miRNAs in their native state may retain the capacity for miRISC-mediated

193 eds along routes that are different from the native state mechanism explored here.

194 d, lack lipids, and show discrepancies with "native states" mostly obtained in detergent micelles.

195 The synchronization of native state motions as they transition between microsta

196 view, using diffusion-precipitation assays, native state MS, isothermal titration calorimetry, singl

197 In the native state, mu1 determines the environmental stability

198 tions in vitro, a small fraction reaches the native state (N) with kobs approximately 0.6 min(-1), wh

199 t is proposed to differ in topology from the native state (N).

200 Apart from the native state, native-like and molten-globule states have

201 Here, we describe the native state, observable intermediate state, and unfolde

202 It has been suggested that the native state of a protein acts as a kinetic hub that can

203 The native state of alpha-synuclein was probed by gel filtra

204 icroscopy studies are unable to preserve the native state of chemically reactive and beam-sensitive b

205 molecular mechanisms of oligomerization, the native state of GAAPs in living cells and the functional

206 ermodynamic and structural properties of the native state of HisF.

207 ccessible conformers in equilibrium with the native state of immature SOD1 (apoSOD1(2SH)).

208 d hydrodynamic techniques to investigate the native state of PCM components in the cytoplasm.

209 ter extraction method appeared to retain the native state of pectin molecules compared with acid and

210 eciphering the mechanisms that stabilize the native state of proteins, as well as those that maintain

211 consider flexibility of each residue in the native state of proteins.

212 ed mutation (F198S) known to destabilize the native state of PrP was also found to stabilize the beta

213 umbersome probes can significantly alter the native state of receptors.

214 This property may confer on the native state of such proteins significant elasticity.

215 el of the complex that well approximates the native state of the complex.

216 ts that the osmolyte glycerol stabilizes the native state of the human cardiac I27 titin module again

217 It appears, therefore, that the native state of the prion protein undergoes substantial

218 tituted membrane systems may not reflect the native state of the protein in vivo.

219 a transition state that is distinct from the native state of the protein.

220 various paramagnetic species present in the native state of these samples and understand their molec

221 The native state of this 35-residue polypeptide contains sev

222 uding natural polyphenols, which protect the native state of TTR by specifically interacting with its

223 The methods used maintained the endogenous native state of tumours and we exploited this to investi

224 The functional native states of globular proteins become unstable at lo

225 Unfolded states of proteins and native states of intrinsically disordered proteins (IDPs

226 shifts about conformational fluctuations in native states of proteins.

227 rmined cryo-EM structures of major and minor native states of the complex, differing in the position

228 tive molecular dynamics investigation of the native states of wild-type and Z alpha1-AT, revealing a

229 exhibit CCS values similar to those of the "native-state" of the protein.

230 MBP then folds to the native state on a longer time scale (~100 s), suggestive

231 n of a random coil unfolded state toward the native state on an energy surface rough with local minim

232 cedures often alter the molecules from their native state on the cell; 2) Long processing time before

233 ns exhibit levels of order comparable to the native state on the nanosecond time scale.

234 denaturant and is in slow exchange with the native state on the nuclear magnetic resonance time scal

235 an essential lysine can be expressed in its native state only upon genetic incorporation of N--acety

236 ne to aggregation are thought to reach their native state optimally.

237 -ray crystallography, NMR, dynamics studies, native state pKa measurements, and thermodynamic analysi

238 MPC in its native state possess very poor solubility, therefore, su

239 with mutations that, destabilizing proteins native state, promote their aggregation.

240 acquisition of distinct determinants of the native state, provides unprecedented evidence for a sequ

241 With the native state, putrescine2+ is a weak competitor when the

242 SAXS revealed increases in the native state radius of gyration, R(g), of 2.2% to 4.1%,

243 they increase the fraction that reaches the native state rather than forming the misfolded state.

244 ctra from biogenic guanine crystals in their native state, resolving their characteristic C-H, N-H an

245 ional reconstructions of whole cells in near-native states revealed a thin PG-like layer extending fr

246 tools to obtain receptor complexes in a near-native state, revealing insights into the gross conforma

247 deduced a free-energy surface that exhibits native-state ruggedness with apparently similar barrier

248 ssist proper folding, Cdk1-cyclin B1 acts on native state separase.

249 Mass spectrometry acquired in the native state showed that the onset of wild-type beta2m o

250 In the non-native state (significantly explored at 380 K), the prob

251 show that when variants have similar reduced native-state stabilities, the location of the mutation (

252 consistent with the P14 mutations perturbing native state stability and shifting an allosteric equili

253 al data, after correcting for differences in native state stability.

254 The correlation between the native-state stability and compactness of the denatured

255 fect of a mutation that has minor effects on native-state stability at the domain interface (I56V) an

256 utation results in only minor changes to the native-state stability of lysozyme.

257 isfolding intermediates is an alternative to native state stabilization and requires substoichiometri

258 Native state stabilizers are promising drugs to treat TT

259 the reactive center loop P14 residue from a native state stabilizing interaction with the hydrophobi

260 ach it is possible to fold proteins to their native states starting from extended structures.

261 of the C-terminal helix, which may alter the native-state structural ensemble of the molecule, leadin

262 mic-scale insight into the attributes of the native state that encodes the broad specificity, we used

263 keleton and its associated proteins in their native state that is also compatible with current mass s

264 ly high barrier separating the denatured and native states that folding appears cooperative even unde

265 rt into an unusual structure from its folded native state; that is, amyloid fibrils analogue to those

266 In its native state, the last 56 amino acids of the ectodomain

267 ss in which the intermediate converts to the native state through a downhill barrierless transition.

268 template-based protein models closer to the native state through conformational sampling.

269 show that Gbeta interacts with CCT in a near-native state through interactions of the Ggamma-binding

270 e funneled landscape, proteins fold to their native states through a stochastic process in which the

271 hysical agents can guide Env from the stable native state to perturbation-sensitive forms and modulat

272 nd located the conformational changes in the native state to the constant domain of the light chain (

273 It populated the same native state, transition state, and folding intermediate

274 ents show that the variant exists in a major native state, two minor native-like states, and two loca

275 HSD2 from pH 7.4 to 4.5, possibly due to the native state unfolding to the intermediate ion core stat

276 allosteric mechanism of Par-6 suggests that native-state unfolding may be essential for the function

277 ers in water were directly observed in their native state using cryo-TEM measurements.

278 ediate, and selective destabilization of the native state using point mutations.

279 temperature near equilibrium dynamics of the native state, using phosphoglycerate kinase as model pro

280 ween the native state and the average of non-native states versus the roughness measured by the varia

281 roEL-MSG complex led to the formation of the native state via a compact intermediate with the rate se

282 forms a "prepore" structure that attains the native state via a conformational switch.

283 These native state volume fluctuations were found to correlate

284 This enhancement in the non-native state was due to glycine, as demonstrated by simu

285 ainst exchange with solvent deuterium in the native state was found in a subset of buried positions i

286 e contact far from the native interface, the native state was reached by dissociation and eventual re

287 of the band that corresponds to loops in the native state, whereas the rest of the regular elements o

288 sualization of cellular organization in near-native state which plays important roles in the field of

289 s a well-defined free energy well around the native state, which makes the interaction stable.

290 lows its client protein Im7 to fold into the native state while continuously bound to the chaperone.

291 As aggregation can be triggered by non-native states, whose population is not necessarily relat

292 We also characterize a near-native state with a highly mobile transmembrane helix 5

293 to the native state, although they reach the native state with approximately the same rate constant a

294 bel-free imaging of complex samples in their native state with minimal to absolutely no sample prepar

295 By comparing the stability of the native state with reference to the knotted and unknotted

296 It compares the energy distributions of the native state with respect to structural decoys.

297 d imaging of erythrocyte morphology in their native states with a nm path length sensitivity.

298 details of the primary cell wall in its near-native state, with implications for microfibrils motions

299 (ABPP) of propofol-binding proteins in their native state within mouse synaptosomes.

300 nsional structures of the chloroplast in its native state within the cell.