ライフサイエンスコーパス: natural amino acid

1 SH3 and PxxxRxxKP for cSH3 (x represents any natural amino acid).

2 xtent to which they prevent incorporation of natural amino acids).

3 >2.3-fold improvement over the best possible natural amino acid.

4 pyrrolysine is the 22nd genetically encoded natural amino acid.

5 that substituted its leucine core with each natural amino acid.

6 d, but it is still a challenge to obtain non-natural amino acids.

7 d to replace S140 or V141 with several other natural amino acids.

8 ent conjugation in the presence of the other natural amino acids.

9 ude extracts in the face of competition from natural amino acids.

10 d to make (F)Cbz derivatives of 18 of the 20 natural amino acids.

11 coded residue is distinct from any of the 21 natural amino acids.

12 ntially take up the analog compared with the natural amino acids.

13 by amide bonds and not being limited to the natural amino acids.

14 ially activates the analog compared with the natural amino acids.

15 f the form AcWLL-X-LL, where X is one of the natural amino acids.

16 synthetic peptides, including ones with non-natural amino acids.

17 , with deeper minima than those observed for natural amino acids.

18 detection of tryptophan over other nineteen natural amino acids.

19 ene linker between cysteine and nucleophilic natural amino acids.

20 ch Trp496 was substituted by natural and non-natural amino acids.

21 ng hybrids of l-sulfono-gamma-AApeptides and natural amino acids.

22 rolysin can be used to identify 13 of the 20 natural amino acids.

23 in silico modeling and incorporation of non-natural amino acids.

24 ore side-chain diversity well beyond that of natural amino acids.

25 orged starting from abundant and inexpensive natural amino acids.

26 acids in length, including d- and other non-natural amino acids.

27 bone, an arrangement that is not found among natural amino acids.

28 r enones with azomethine ylides derived from natural amino acids.

29 th a variety of side chains corresponding to natural amino acids.

30 rk for the design of peptides containing non-natural amino acids.

31 les and the mild synthesis of thiazoles from natural amino acids.

32 ts for the enantioselective synthesis of non-natural amino acids.

33 iling important building blocks of life: the natural amino acids.

34 ne 43 of CD4 and cannot be fully accessed by natural amino acids.

35 mmon difference between the masses of the 20 natural amino acids.

36 specific unlabeled peptides composed only of natural amino acids.

37 acyl-1,omega-amino acids (5, 9a-f, 14a-f) of natural amino acids (10a,b) and of dipeptide (10c) are p

38 bled the display of libraries composed of 20 natural amino acids (20AA).

39 the direct incorporation of a fluorescent un-natural amino acid, (7-hydroxy-4-coumarin-yl)-ethylglyci

40 The combination of a natural amino acid (AA) (l-Glu, l-Tyr, and l-Lys) and te

41 simulation for the most active analogue with natural amino acids, Ac-V4W/H9A-NH(2).

42 of peptides, and the early evolution of the natural amino acid alphabet.

43 well as peptides composed of natural and non-natural amino acid analogs, thereby enabling screens in

44 Pyrrolysine (Pyl), the 22nd natural amino acid and genetically encoded by UAG, becom

45 By genetic incorporation of a non-natural amino acid and two compatible bioorthogonal chem

46 Given the fact that Phe CN is a non-natural amino acid and, thus, amenable to incorporation

47 elivery peptides are exclusively composed of natural amino acids and are consequently readily degrada

48 a method using genetic incorporation of non-natural amino acids and bio-orthogonal chemistry to site

49 carboxylate esters belong to the L-series of natural amino acids and catalyze asymmetric Michael addi

50 prepared starting from diamines derived from natural amino acids and commercially available aminoalky

51 carnitines, while deplete in the most common natural amino acids and derivatives.

52 The peptide is composed entirely of natural amino acids and exhibits a marked (42%) change i

53 including quenching interactions with select natural amino acids and Forster resonance energy transfe

54 This engineered antigen contains two non-natural amino acids and has an alpha-S-glycosidic bond,

55 of vMIP I, a chemokine that contains all 20 natural amino acids and has two disulfide bonds.

56 id within the interacting peptide for all 20 natural amino acids and identified novel binding sequenc

57 e Gap1p sorting, because the addition of all natural amino acids and many amino acid analogs to the g

58 riety of reactions using a limited number of natural amino acids and metallocofactors.

59 orial library containing 14 unnatural and 19 natural amino acids and screened this library for strept

60 y the types of binary potentials observed in natural amino acids and show that there is a strong bias

61 f the method allowed the synthesis of all 20 natural amino acids and their NCL reactions.

62 theoretical tryptic peptides composed of 20 natural amino acids and with masses limited to 3.5 kDa.

63 s with post-translational modifications, non-natural amino acids, and disulfide bonds.

64 t one, or at most, two, point mutations with natural amino acids are all that is required to impart v

65 Electronic nanostructures made from natural amino acids are attractive because of their rela

66 Fluorine-containing analogues of natural amino acids are becoming increasingly important

67 However, most materials derived from natural amino acids are electronically insulating.

68 Peptides constructed with the 20 natural amino acids are generally considered to have lit

69 Non-natural amino acids are increasingly used as building bl

70 Hindered, non-natural amino acids are of interest because they import

71 7%) have been achieved by using primary free natural amino acids as catalysts and Et(2)AlCN as nucleo

72 es in a 1 cm(2) area on a microchip using 20 natural amino acids as well as synthetic amino acid anal

73 pounds are the boronic acid analogues of the natural amino acids aspartic acid and glutamic acid with

74 as substitutions more closely related to the natural amino acid at appropriate positions.

75 osition 460 to His, which corresponds to the natural amino acid at the homologous position in STAT6,

76 ipeptides 33a,b and 35a,b, each containing a natural amino acid at the N-terminus, avoids the low cou

77 ssay quantifies insertion energetics for all natural amino acids at 27 positions across the membrane,

78 analogues have tryptophan or fused-ring non-natural amino acids at position 4 and alanine or an unbr

79 The fused-ring non-natural amino acids at position 4 contribute to the form

80 Systematic analysis of all 20 natural amino acids at position E262 shows that the natu

81 cells, we substituted histidine residues for natural amino acids at the cytoplasmic ends of the TM he

82 Such libraries have incorporated non-natural amino acids at the expense of natural amino acid

83 ilar to those observed for substitution with natural amino acids at the same site but had little effe

84 ethodology, the undesirable incorporation of natural amino acids at the suppression site.

85 was used to evaluate the full complement of natural amino acids at these positions, and resulted in

86 ll libraries, including both natural and non-natural amino acids, based on the previous mutational si

87 minimizing such perturbations is to use non-natural amino acid-based FRET pairs.

88 hieve this involves the utilization of a non-natural amino acid by the cell's native translational ap

89 nopore technology, which aims to identify 20 natural amino acids by reading the ionic current differe

90 ed non-natural amino acids at the expense of natural amino acids by reassigning their codons.

91 have also shown that thioamide analogues of natural amino acids can be incorporated into full-sized

92 Non-natural amino acids can be incorporated into synthetic h

93 Thioamide analogues of the natural amino acids can be placed in a variety of locati

94 tural amino acids in conjunction with the 20 natural amino acids can be used as building blocks to co

95 ally, our algorithm is compatible with every natural amino acid, can evaluate both homotrimers and he

96 ptide, additional modifications with the non-natural amino acids citrulline, hydroxyproline, and d-al

97 ransporter-1 (LAT1) but is less sensitive to natural amino acid competition.

98 ompared to random sequences with uniform and natural amino acid compositions, as observed by a lower

99 Fitness studies in quails revealed that some natural amino acids conferred an in vivo replication adv

100 A non-natural amino acid containing an aminooxy side chain was

101 ys22 in ShK with the positively charged, non-natural amino acid diaminopropionic acid (ShK-Dap22) and

102 uch as glutathione and cysteine and other 19 natural amino acids do not interfere with such CSH-induc

103 Here we show that taurine, a natural amino acid, drastically boosts the cellular upta

104 selective chemical modifications directly on natural amino acids, especially at the beta-position, re

105 Asn-Xaa-Thr/Ser sequon, where Xaa can be any natural amino acid except proline, for N-linked glycosyl

106 P3, and P4 positions) were varied across all natural amino acids (except cysteine and methionine).

107 We have previously improved a natural amino-acid form of a scorpion toxin-derived CD4-

108 codons for the biosynthetic insertion of non-natural amino acids has been developed.

109 unique groups into proteins by means of non-natural amino acids has numerous applications in protein

110 Arginine, one among the 20 most common natural amino acids, has a pivotal role in cellular phys

111 of pseudopeptidic macrocycles containing non-natural amino acids have been synthesized.

112 structure of peptides or proteins containing natural amino acids; however, a deficiency in the abilit

113 ectrophilic reagents enables modification of natural amino acids in a single transformation but proce

114 can lead to contaminating protein forms with natural amino acids in place of the ncAA.

115 ino acid redox cofactor with the full set of natural amino acids in protein design.

116 Nine of the 20 natural amino acids in proteins can be phosphorylated, b

117 ifts and confirm that the sampling of the 20 natural amino acids in this peptide series is be far fro

118 with a desired unnatural amino acid, but no natural amino acids, in eukaryotic cells.

119 By pairing non-natural amino acid incorporation and cell surface click

120 by combining amber suppression-mediated non-natural amino acid incorporation and tubulin overexpress

121 alanine (4 F-Phe), through site specific non-natural amino acid incorporation.

122 lso named eosinophil cationic protein-by non-natural amino acids increases stability in human serum.

123 codon has the potential to incorporate a non-natural amino acid into the polypeptide chain.

124 aturation of macrocyclic peptides taking non-natural amino acids into consideration.

125 Incorporation of non-natural amino acids into proteins in vivo expands the sc

126 -tRNA pairs that selectively incorporate non-natural amino acids into proteins; our method uses paral

127 hile genetic engineering can incorporate non-natural amino acids into recombinant proteins, regiosele

128 an be engineered to introduce synthetic "non-natural" amino acids into nonribosomal peptides.

129 ing the fact that the average mass defect of natural amino acids is larger than that of oxidized Met.

130 However, high-fidelity incorporation of non-natural amino acids is precluded in this system by misch

131 e hismox is a chiral ligand derived from the natural amino acid l-histidine, are replaced by CH3NH3(+

132 nels with thio-alkyl chains derived from the natural amino acid l-methionine (1) has been rationally

133 xamide-based metalloligands derived from the natural amino acids l-serine and l-methionine.

134 ter carbonyl stretching vibration of two non-natural amino acids, L-aspartic acid 4-methyl ester and

135 he LH receptor, conversion of Ser-277 to all natural amino acids led to varying degrees of receptor a

136 sition, enabled by a chiral mono-N-protected natural amino-acid ligand, and subsequent relay to the r

137 -CoA (MCPF-CoA), a metabolite derived from a natural amino acid, (methylenecyclopropyl)glycine, again

138 age functionalization of pharmaceuticals and natural amino acid modification, and three reactions wer

139 tro, revealing that the incorporation of non-natural amino acids modulated (either positively or nega

140 We used site-directed non-natural amino acid mutagenesis to engineer rhodopsin wit

141 wn crystal structure, and 24 natural and non-natural amino acid mutant variants of these peptides.

142 An alpha-methyl, non-natural amino acid (NNAA) building block equipped with a

143 show here the genetic incorporation of a non-natural amino acid (NNAA) followed by chemoselective alb

144 ethod for site-specific incorporation of non-natural amino acids (nnAAs) into proteins in which the o

145 r codon suppression for the insertion of non-natural amino acids (nnAAs) is limited by competition wi

146 d amino-acid diversity that preserves all 19 natural amino acids (no methionine) and adds 6 non-natur

147 perior selectivity for Cys over the other 19 natural amino acids (nonthiol-containing).

148 However, the impact of non-natural amino acids on the antibiofilm and immunomodulat

149 distributions of peptides composed of the 20 natural amino acids, oxidized Met, and phosphorylated Se

150 The incorporation of the non-natural amino acid p-azido-phenylalanine (pAzF) enables

151 ategy that incorporates a photoreactive, non-natural amino acid, p-benzoyl-l-phenylalanine, into vari

152 Here, we report that the non-natural amino acid pipecolinic acid in the P2 position o

153 IMPORTANCE Typically, a natural amino acid polymorphism is found as the wild-typ

154 de proteins with functional variation due to natural amino acid polymorphism.

155 ects of the drug-resistant mutations and the natural amino acid polymorphisms on the Gibbs energy are

156 complete diastereoselectivity achieved with natural amino acid precursors is completely lost with th

157 the reaction rate and the yield of these non-natural amino acid precursors while preserving the remar

158 simple bioorganic peptide glass composed of natural amino acids, presenting a multi-functional mater

159 ng, conformationally disordered chain of the natural amino acids Pro, Ala, and Ser (PASylation)-a bio

160 Recent advances in natural amino acid production have motivated the use of

161 The non-natural amino acids (R)- and (S)-2-amino-3-fluoro-2-meth

162 Serine, one of the most abundant natural amino acids, remains underutilized due to the in

163 substitutions together with natural and non-natural amino acid replacements.

164 The spin label is assembled in situ from natural amino acid residues and a metal salt, requires n

165 f protein complexes, often direct mimicry of natural amino acid residues does not lead to potent inhi

166 We replaced natural amino acid residues in Ste2p with Bpa by enginee

167 de bonds within peptide backbones; modifying natural amino acid residues; and rigidification.

168 identified a combination of natural and non-natural amino-acid residues that was used to generate hi

169 from the random coil chemical shifts of the natural amino-acid residues.

170 incorporated into the hydrophobic core using natural amino acids, restricting water access to the lan

171 l optimization involving introduction of non-natural amino acids resulted in a lead Bicycle that demo

172 l amino acids (no methionine) and adds 6 non-natural amino acids, resulting in the highest sequence c

173 library, which collectively contained all 20 natural amino acids, revealed that aryl di-O-acetyl comp

174 tem, we describe the utilization of the 21st natural amino acid selenocysteine for the generation of

175 ort, with post-import excision restoring the natural amino acid sequence.

176 igomers that manifest the folding encoded in natural amino acid sequences is a significant challenge.

177 s are presently limited by the repertoire of natural amino acid side chain and carbohydrate functiona

178 The transfer free energies of the twenty natural amino acid side chains from water to phospholipi

179 e., a "hydrophobicity scale") for the twenty natural amino acid side chains measured in the context o

180 ally those with both secondary structure and natural amino acid side chains that coordinate to metal

181 A natural amino acid substitution in the human immunodefic

182 robe the redox properties of CcP Y191 by non-natural amino acid substitution, altering the ET driving

183 contain polar N-terminal extensions and non-natural amino acid substitutions at positions 4 and 9.

184 The catalytic effect of natural and non-natural amino acid substitutions at the P1 position in a

185 Natural amino acid substitutions found in Aglaia eIF4As

186 rate and product specificities by fixing the natural amino acid substitutions with high frequency.

187 mes is generated by breeding combinations of natural amino-acid substitutions from the cyclic into th

188 amino acid (unAA) substrate from unfavorable natural amino acid substrates.

189 The utilization of natural amino acids such as Met, SeMet, and His leads to

190 tuents, which mimic the side chains found in natural amino acid systems.

191 s with near-normal sensitivity, although the natural amino acids tested other than Pro produce recept

192 where we identify sites to incorporate a non-natural amino acid that can be chemically modified to mo

193 lained as a result of glycine being the only natural amino acid that can play this role.

194 ate or aspartate substitutions, there are no natural amino acids that can mimic phospho-tyrosine.

195 his knowledge gap arises from the absence of natural amino acids that can mimic these PTMs and the la

196 tegy for designing short peptides containing natural amino acids that function as artificial TADs.

197 abled us to define a nucleophile composed of natural amino acids that is ligated efficiently to the s

198 This paper reports the use of natural amino acids, the tripeptide beta-strand mimic Ha

199 ieve exquisite control and diversity from 20 natural amino acids, there is considerable scope for the

200 e selection of peptides that incorporate non-natural amino acids, thus expanding the chemical diversi

201 ay be generally expanded to target different natural amino acids, thus providing diversity and flexib

202 orporation into membrane proteins of the non-natural amino acid TOAC (2,2,6,6-tetramethyl-piperidine-

203 d so far, to be best of our knowledge, using natural amino-acid tryptophans to form networks for both

204 The behavior of 20 natural amino acids under the basic permethylation condi

205 netic code for specification of multiple non-natural amino acids, unique codons for these novel amino

206 All living organisms encode the 20 natural amino acid units of polypeptides using a univers

207 eses of highly enantiomerically enriched non-natural amino acids using a simple chiral amido-thiourea

208 ma-aryl-alpha-amino acids were prepared from natural amino acids using this method.

209 , a macrocyclic peptide 22 involving two non-natural amino acids was evolved showing increased target

210 Only natural amino acids were used as probes, and thus possib

211 m provides a method for incorporation of non-natural amino acids which avoids the use of stop codons.

212 d compare the current blockage of each of 20 natural amino acids, which are all far from producing a

213 ng aminoacyl tRNAs that are charged with non-natural amino acids, which could be incorporated into pr

214 d calculating the binding energies of the 20 natural amino acids, which shows that tyrosine binds the

215 , and SGTA, allowing the installation of non-natural amino acids with stereochemical control.

216 Substitution of natural amino acids with their aza-amino acid counterpar

217 tructurally very similar to the peptide with natural amino acids, with an rmsd difference of only 1.3

218 rate multiple unnatural amino acids (but not natural amino acids), would eliminate the need to genera