ライフサイエンスコーパス: nested PCR

1 (hence, the term merged tandem-nested or M/T-nested PCR).

2 ymptomatic staff and patients were tested by nested PCR.

3 on, and junction fragments were amplified by nested PCR.

4 ules for each clone type using two rounds of nested PCR.

5 r detection of adenoviruses was performed by nested PCR.

6 plified from individually dissected cells by nested PCR.

7 , viral genomes were no longer detectable by nested PCR.

8 HTLV-1 tax had been periodically detected by nested PCR.

9 r the presence of RRV by virus isolation and nested PCR.

10 rious depths and the dorsum of the tongue by nested PCR.

11 2 and 6 of the PIG-A gene were amplified by nested PCR.

12 be used to detect Cryptosporidium parvum by nested PCR.

13 and tested them for pig DNA and PERV DNA by nested PCR.

14 -negative samples (88%) were negative in the nested PCR.

15 higher than 5 parasites/uL when compared to nested PCR.

16 blood of subclinically infected mice by the nested PCR.

17 A, 25 patients (26%) were positive by the RT-nested PCR.

18 The samples were tested by nested PCR.

19 plified in Ehrlichia canis-infected cells by nested PCR.

20 dorferi from infected ticks was amplified by nested PCR.

21 00% when discrepant samples were retested by nested PCR.

22 udonana colonies screened positive for HR by nested PCR.

23 is currently accomplished using serology or nested PCR.

24 HCV and HBV was confirmed through ELISA and nested PCR.

25 between July and December for microscopy and nested PCR.

26 city of LAMP results, compared with those of nested PCR.

27 multiplex assay platforms without the use of nested PCR.

28 s needed for sample processing and multiplex nested PCR.

29 to perform two to three successive rounds of nested PCR.

30 in 81 of 180 (45%) of individuals using semi-nested PCR.

31 e-specific RT-PCR and real-time quantitative nested PCR.

32 ng HIV PT and RT sequences were amplified by nested PCRs.

33 61%; 95% CI, 53 to 69%; P = 0.007) and ITS-1 nested PCR (54%; 95% CI, 45 to 62%; P < 0.001); real-tim

34 This demonstrated that the nested PCR achieved very nearly the maximum theoreticall

35 Nested PCR also detected M. pulmonis in 14 of 20 (70%) p

36 Nested PCR also detected M. pulmonis in 21 of 61 (34%) C

37 cted to nucleotide sequence analysis using a nested PCR amplification approach for each gene.

38 were determined after reverse transcriptase-nested PCR amplification of viral RNA directly from rumi

39 The sensitivity of the nested PCR amplification reaction was one organism.

40 A second round of nested PCR amplification was performed in the presence o

41 The nested PCR amplified integrated proviral DNA in nucleic

42 Two-stage nested PCR analysis and DNA sequencing confirmed the int

43 Nested PCR analysis at the SAG2 locus provides rapid ass

44 The multiplex nested PCR analysis described here will be useful for an

45 y the 50% tissue culture infectious dose and nested PCR analysis of proviral DNA.

46 Multiplex nested PCR analysis was used to genotype parasites in ce

47 In summary, RT-nested PCR and a commercially available RT-PCR assay for

48 tudy was to compare the sensitivities of the nested PCR and cell culture with that of the indirect fl

49 Nested PCR and DNA sequencing analyses confirm that SECH

50 n fragments into 96-well plates, followed by nested PCR and DNA sequencing, was used to determine the

51 of E. canis nonendemicity) were examined by nested PCR and immunofluorescent-antibody (IFA) test.

52 ce of selected organisms were examined using nested PCR and multiplexed bead-based flow cytometry.

53 We present a nested PCR and nanopore sequencing protocol that allows

54 virus and shellfish samples, application of nested PCR and nucleotide sequencing, and increased know

55 HHV8-seropositive donor samples by in-house nested PCR and quantitative real-time PCR assays, respec

56 assay offers an alternative to conventional nested PCR and restriction fragment length polymorphism

57 action (PCR) and sequencing, and for RSTs by nested PCR and restriction fragment length polymorphism

58 fic polymerase chain reaction (RS-PCR), semi-nested PCR and RNase protection assay.

59 Nested PCR and Southern blot analysis confirmed the iden

60 se results by using single-cell primer-based nested PCRs and Sanger sequencing.

61 HGE agent no. 13 isolate as the antigen, by nested PCR, and by culture.

62 d studied by immunochemistry, real-time PCR, nested PCR, and in situ hybridization to identify NKT ce

63 clinic and compared with expert microscopy, nested PCR, and quantitative PCR (qPCR).

64 enes from single E4+ cells were amplified by nested PCR, and the amplified products were sequenced di

65 The Pfmdr1 D1246Y allele was amplified via nested PCR, and the mutation was detected using the rest

66 rmed PB, and RQ-PCR was more reliable, while nested PCR appeared applicable to a larger number of pat

67 A new three-phase, double-nested PCR approach allowed robust melting temperature a

68 In vivo, as detected by a very sensitive nested PCR approach, methylation of the discrete AP-2alp

69 By using an inverse nested PCR approach, we found that the VPI-2 region can

70 thylated DNA, which normally would require a nested PCR approach.

71 However, a nested-PCR approach using non-degenerate MIH-specific pr

72 vity and specificity were calculated using a nested PCR as the gold standard and the novel primer set

73 by comparing with an 18S ribosomal RNA-based nested PCR as the gold standard.

74 jority of studies have used highly sensitive nested PCR as the only method of detection, more robust

75 el and comparator RDTs, using microscopy and nested PCR as the reference standards.

76 lls (PBMCs) from 68 of 101 patients (67%) by nested PCR, as compared with 8 of 218 (3.7%) healthy con

77 The new 23S-5S IGS nested PCR assay amplified all 11Rickettsiaspp., but the

78 We compared a nested PCR assay and microscopic examination of Giemsa-s

79 By using a sensitive nested PCR assay and targeting the nuclear 18S rDNA-ITS1

80 gonucleotide primer design and seminested or nested PCR assay design were used to enhance the breadth

81 A multiplex nested PCR assay for detection of F. tularensis, B. anth

82 The nested PCR assay had an overall sensitivity of 42.5%, a

83 MRV gag and env and one sensitive, published nested PCR assay targeting env.

84 Here we describe a fluorescence-monitored, nested PCR assay that is able to quantify the relatively

85 This study describes the development of a nested PCR assay that uses a unique element (ISMap02) fo

86 lot assay to detect antibodies to NWM SFV, a nested PCR assay to detect NWM SFV DNA, and a beta-galac

87 A newly described nested PCR assay was capable of amplifying genomic DNA f

88 A sensitive and specific nested PCR assay was developed for the detection of gran

89 A nested PCR assay was developed to analyse the appearance

90 In this study, a nested PCR assay was developed to detect C. parvum DNA d

91 A novel nested PCR assay was developed to detectRickettsiaspp. i

92 A nested PCR assay was used to analyze the appearance of t

93 nodes of seven (20%) deer were positive in a nested PCR assay with E. chaffeensis-specific primers.

94 detect RNA extracted from the samples: an RT-nested PCR assay with primers derived from the 5' noncod

95 mal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading f

96 an indirect fluorescent antibody test and a nested PCR assay, respectively.

97 We used a nested PCR assay, which reproducibly detected a 10(4)- t

98 rotoxin detection by cytotoxin assay and the nested PCR assay.

99 done using an in-house reverse transcriptase-nested PCR assay.

100 1/8 using the COBAS assay and 6/8 using the nested PCR assay.

101 In the present study, a nested-PCR assay targeting the Tso31 gene was developed

102 was measured using a quantitative real-time nested-PCR assay, and the specificity of directed integr

103 Using a nested-PCR assay, we found that both DeltaM83 and DeltaM

104 s comparable to that of previously described nested PCR assays (A. phagocytophilum, 16S rRNA; B. burg

105 s real-time PCR assay offers advantages over nested PCR assays and may improve the detection of C. pn

106 showed both assays to be more sensitive than nested PCR assays currently in use at the CDC.

107 me PCR demonstrates greater sensitivity than nested PCR assays in FFPE tissues and provides an effect

108 monkeys were evaluated for XMRV infection by nested PCR assays with nucleotide sequence confirmation,

109 mia (CMV-Ag) assay, one or more in-house CMV nested PCR assays, and/or patient evaluation and follow-

110 compared to the results of culturing and two nested PCR assays, targeting the 16S rRNA and ompA genes

111 Oligonucleotide primers were developed for nested PCR based on Chlamydia, Ureaplasma, and Neisseria

112 We describe here a nested PCR-based strategy for genome walking to extend a

113 with parasitemia levels detectable by 3-well nested PCR but very low or undetectable by qPCR.

114 . pulmonis strain, 14 (19%) were positive by nested PCR, but only 2 of 72 (2.8%) were positive by cul

115 ands appeared in most cases after performing nested PCR casting doubt on the physiologic relevance of

116 of the ASCs are then amplified by RT-PCR and nested PCR, cloned into expression vectors and transfect

117 Nested PCR cloning from an S. gordonii library enabled t

118 Nested-PCR cloning enabled the isolation of a 324-bp-lon

119 r the detection of both point mutations uses nested PCR combined with restriction enzyme digestions,

120 Strain-specific nested PCR confirmed that the superinfecting strain was

121 Having established that the nested PCRs could detect single molecules of target sequ

122 f 20 replicates, using 12 outer and 28 inner nested PCR cycles, with an intervening UNG digestion ste

123 The RS-PCR and the semi-nested PCR demonstrated products that co-migrated with t

124 f the 5'NC and NS3 sequences amplified by RT-nested PCR demonstrated that all but two positive patien

125 The Tso31 nested PCR described here might be a useful tool for the

126 Conventional nested PCR detected herpesviral DNA in brain tissue samp

127 The single-tube nested PCR did not amplify P. carinii isolated from rats

128 ipts detectable by reverse transcription-PCR/nested PCR (e.g., PDX-1, PAX-4, PAX-6, Nkx2.2 and Nkx6.1

129 By the nested PCR, E. risticii was detectable in the blood and

130 The real-time VD4 assay and one nested PCR each detected C. pneumoniae in a single, but

131 gene (CP1-CP2/CPC-CPD) was used to perform a nested PCR, followed by confirmation of the findings wit

132 Each nucleus was assayed by nested PCR for cccDNA and for cellular IFN-alpha genes a

133 on the 16S rRNA gene relative to that of the nested PCR for detection of E. chaffeensis in infected D

134 HIV RNA could not be detected in plasma, and nested PCR for HIV RNA and DNA on bulk PBMCs and sigmoid

135 These findings were confirmed by nested PCR for SIVgag DNA in brain and RT-PCR for viral

136 The results were compared to those of a nested PCR for the detection of HCMV glycoprotein B DNA

137 ere consistently found within lesions, and a nested PCR for the rRNA gene also demonstrated the prese

138 oratory between May, 1994, and May, 1996, by nested PCR for viruses associated with CNS infections in

139 ay (ELISA) from Techlab, using real-time and nested-PCR for Entamoeba species to resolve any discrepa

140 rimer pairs, HPV sequences were amplified by nested PCR from DNA isolated from cervical smear samples

141 Nested PCR from spiked urine samples detected 1 to 10 co

142 The nested PCR generated a strong signal from a minimum of 0

143 ication method, and for HCMV and EBV-1 using nested PCR identification.

144 mpared among LAMP-QCM, conventional LAMP and nested PCR in 50 cervical cancer tissues.

145 es, we detected JCV regulatory region DNA by nested PCR in 6/19 (32%) HIV-positive PML patients, 2/11

146 d sensitivity similar to that of single-well nested PCR in a United Kingdom reference laboratory.

147 rent infections with CAV-1, as detected by a nested PCR, in a range of samples, including liver, kidn

148 xpression by reverse transcriptase-dependent nested PCR, including DNA sequence analysis, in situ hyb

149 Semi-nested PCR increased detection sensitivity even further.

150 e virus-cell junctions detectable by inverse nested PCR (invPCR).

151 The study indicated that the nested PCR is as sensitive as culture for detecting infe

152 Our results indicate that the nested PCR is highly sensitive and specific for detectio

153 Our results demonstrate that the single-tube nested PCR is ideally suited for (i) diagnostic testing

154 Nested PCR is not an adequate method for identifying DNA

155 We conclude that nested PCR is superior to single PCR or culture for dete

156 set of four highly sensitive and polymorphic nested PCR markers.

157 By use of a very sensitive nested PCR method targeting part of the strongly conserv

158 Here, a nested PCR method targeting the internal transcribed spa

159 d respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequen

160 Furthermore, a new nested-PCR method for the detection of morbillivirus is

161 The results were compared to those of a nested-PCR method targeting the insertion sequences IS48

162 A consensus nested-PCR method was designed for investigation of the

163 I: 90.40-100%) compared to the gold standard nested-PCR method.

164 of which were not recovered by commonly used nested-PCR methods.

165 eritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FI

166 Previously, boar semen was tested by a nested PCR (nPCR) assay which was compared to the "gold

167 ide-by-side comparison with the conventional nested PCR (nPCR) assay, 248 samples were screened in tr

168 up to 500 m outside hotspots, determined by nested PCR (nPCR) at baseline and 8 wk (16 June-6 July 2

169 pared RTQ-PCR to microscopy of blood smears, nested PCR (nPCR), and parasite circulating-antigen (CAg

170 Nested PCRs (nPCRs) for amplification of Neisseria menin

171 and evidence of Pneumocystis colonization by nested PCR of BAL fluid.

172 Pooled Chelex extraction of DNA, followed by nested PCR of cytochrome b, was the optimal strategy, al

173 The primers used for nested PCR of the HIV-1 pol gene amplified templates fro

174 esting for HIV, including plasma HIV RNA and nested PCR on bulk peripheral blood mononuclear cells (P

175 Peptide expression was verified using nested PCR on template cDNA derived from mRNA extracted

176 k/ ratios were detected by flow cytometry or nested-PCR or nephelometry in 4% Group A versus 17% Grou

177 The nested PCR outperformed conventional microscopic diagnos

178 Nine sets of nested PCR primers from a 2.6-kb region of the hepatitis

179 ied by ligation-mediated PCR (LM-PCR), using nested PCR primers to increase the specificity and sensi

180 nces of contamination were in the context of nested PCR procedures.

181 were alkaline lysed and after two rounds of nested PCR products were recovered and directly sequence

182 The digested nested PCR products were resolved on a 2% agarose gel an

183 ng, silica membrane DNA isolation and a semi-nested PCR protocol improve the analysis of low biomass

184 mic DNA standard, which revealed that a semi-nested PCR protocol represented microbiota composition b

185 bjected to successive amplifications using a nested PCR protocol, followed by sequencing.

186 or the presence of C. pneumoniae DNA using a nested-PCR protocol targeting a species-specific gene se

187 ing primer walking, allele-specific PCR, and nested PCR provide specialized validation and detection

188 from the total DNA of individuals by use of nested PCR reactions, and the resulting 430-bp fragment

189 ol of gene-specific primers followed by semi-nested PCR resulted in a significant increase in sensiti

190 a first reverse primer followed by two semi-nested PCR rounds using primers that are each time neste

191 We developed a reverse transcriptase nested PCR (RT-nPCR) assay for the detection of viral ga

192 In a more sensitive nested PCR, samples from 18 of 30 (60%) MM patients were

193 In this study, we performed nested-PCR screening and identified bufavirus from 12 me

194 transcripts, the primers used for real-time nested PCR spanned the splice sites.

195 A nested PCR specific for the Mycoplasma pneumoniae P1 gen

196 Remarkably, even a highly sensitive semi-nested PCR, specific for the CLL-expressed IGHV1-69/IGHD

197 non we encountered in the development of our nested PCR-SSP typing system for HLA class II alleles.

198 he addition of a primary amplification step (nested PCR-SSP).

199 sites, we conducted Plasmodium cytb-specific nested PCR surveys using blood from water buffalo in Vie

200 taminating proviral DNA was detected using a nested PCR targeting the Alu repeat in human genomic DNA

201 qualitative polymerase chain reaction (PCR) (nested PCR targeting the cytochrome b gene) and quantita

202 months after transplantation by a two-stage nested PCR technique to detect donor MHC HLA DR gene spe

203 We describe a degenerate nested PCR technique with the capacity to detect a broad

204 gocytophila genogroup rickettsiae by using a nested PCR technique.

205 Assays for HIV-1 DNA were done by using nested PCR techniques that amplify HIV-1 gag DNA from bl

206 Taken together, the two nested PCR tests on the subset of 21 culture-positive sa

207 In nested PCR tests using the 16S rRNA gene, the DNA of the

208 The nested PCR that we used can detect 2.4 copies of KSHV se

209 Upon reverse transcription of the RNA and nested PCR, the procedure detected C. albicans "housekee

210 In comparison with nested PCR, the sensitivity and specificity of RealAmp i

211 Compared with 3-well nested PCR, the sensitivity of both LAMP and single-well

212 Compared with nested PCR, the sensitivity, specificity, positive predi

213 Their Igs were amplified from cDNA using nested PCR, then cloned and sequenced.

214 We used semi-nested PCR to amplify TTV DNA from serum samples from 12

215 protein (PifC) from R. sphaeroides, we used nested PCR to clone and characterise the encoding gene,

216 We developed a nested PCR to detect the enterotoxin gene of B. fragilis

217 nneuronal fractions, followed by primary and nested PCR to quantitate VZV DNA at the single cell leve

218 V gene sequence (KS330233) was negative, but nested PCR to yield a final product of 186 bp internal t

219 rimers were then used to perform a two-step (nested) PCR to amplify the K9-specific rat testicular RN

220 roach, hybridization-enriched templates with nested PCR, to detect microclones with Ig alpha/c-myc re

221 cDNA was cloned and sequenced by RT-PCR and nested PCR using degenerated oligonucleotides.

222 for Ehrlichia risticii, the agent of PHF, by nested PCR using primers specific to the 16S rRNA gene.

223 Eleven spore trap samples were analyzed by nested PCR, using oligonucleotide primers designed for t

224 d newly developed 'asymmetry linker-mediated nested PCR walking' (ALN-walking) for CNV breakpoint seq

225 The sensitivity of multiplex nested PCR was >/=25 parasites/ml in amniotic fluid and

226 absolute detection limit of the single-tube nested PCR was 1 organism, while the practical detection

227 the sensitivity of both LAMP and single-well nested PCR was 90%; the microscopy sensitivity was 51%.

228 Nested PCR was also used to detect H. pylori from beneat

229 A nested PCR was applied to amplify a segment of open read

230 araffin-embedded specimens from 37 dogs, and nested PCR was attempted on DNA from 9 fresh tissue spec

231 A single-tube nested PCR was designed and optimized for the detection

232 A nested PCR was developed to amplify the variable region

233 When RT-nested PCR was performed on 10-fold serial dilutions of

234 Nested PCR was performed only in the samples from vascul

235 PCR was essentially negative, a second-round nested PCR was performed, which revealed expression also

236 The nested PCR was repeated on DNA extracted from a differen

237 The nested PCR was tested for sensitivity on 20 samples from

238 ELISA was done on sera samples, and two-step nested PCR was used on extracted DNA to amplify variable

239 Nested PCR was used to amplify exon 15 of the BRAF gene,

240 Nested PCR was used to detect different RV strains, and

241 Species-specific nested PCR was used to detect Treponema amylovorum, Trep

242 Prevalence rates obtained with nested PCR were significantly higher than those obtained

243 cted patients tested positive for gag by non-nested PCR whereas the two other assays did not detect X

244 A single-tube nested PCR which amplifies the internal transcribed spac

245 Malaria was diagnosed by microscopy and Nested PCR, while EBV reactivation was assessed by detec

246 xons flanking the predicted exon, and a semi-nested PCR with a primer that targets the predicted exon

247 We used a nested PCR with Borrelia flagellin gene (flaB) primers a

248 TaqMan compared favorably with nested PCR with key advantages of speed, increased throu

249 Nested PCR with patient IgH allele-specific oligonucleot

250 tuberculosis-specific DNA was amplified in a nested PCR with previously described primers (primers rp

251 sensitive and 93.3% specific compared with a nested PCR with primer set CP1/2-CPC/D for clinical resp

252 proteinase K and tested by a single or fully nested PCR with primers directed against part of the two

253 s assay by comparing the results obtained by nested PCR with those obtained by TaqMan PCR.

254 Nested PCRs with primer sets specific for the viral T pr

255 had a diagnostic accuracy similar to that of nested PCR, with a greatly reduced time to result, and w

256 roducts were used as templates in subsequent nested PCR, with primers that amplify a 186-bp product i