ライフサイエンスコーパス: neuronal NOS

1 ethyl]aminopentyl)-N'-nitroguanidine, TFA, a neuronal NOS inhibitor, was injected at 0.02 or 0.1 mg/1

2 BDNF activates neuronal NOS with the nitrosylated GAPDH/seven in absent

3 isense oligodeoxynucleotide (AS-ODN) against neuronal NOS, and then tested in a light/dark exploratio

4 6 and 17 (R16/17) are required for anchoring neuronal NOS (nNOS) to the sarcolemma.

5 in glycoprotein complex (DGC), which anchors neuronal NOS (nNOS).

6 constitutive NO synthase (NOS) activity and neuronal NOS (nNOS) but not endothelial NOS (eNOS) phosp

7 t found that both NOS enzymatic activity and neuronal NOS (nNOS) protein expression were reduced (P<0

8 AS expression and activity and neuronal NOS expression, as well as l-arginine and NO(x)

9 In addition, CB1R and neuronal NOS were coexpressed in cultured cortical neuro

10 ls in the midline also contained NADPH-d and neuronal NOS, thus suggesting a potential NO-galanin int

11 ends on the activity of both endothelial and neuronal NOS (eNOS and nNOS, respectively).

12 vealed a distinct pattern of endothelial and neuronal NOS expression in the arthritic synovium that w

13 ed to exclude effects on the endothelial and neuronal NOS isoforms.

14 dimerization of iNOS versus endothelial and neuronal NOS suggests that the energetics and kinetics o

15 he structures of inducible, endothelial, and neuronal NOS with and without CaM bound are similar, con

16 ee NOS isoforms--inducible, endothelial, and neuronal NOS--that are composed of an N-terminal oxidase

17 ed disruptions in endothelial NOS (eNOS) and neuronal NOS (nNOS) genes compared with their wild-type

18 Expressions of endothelial NOS (eNOS) and neuronal NOS, but not inducible NOS, were demonstrated i

19 with targeted deletion of genes for HO2 and neuronal NOS.

20 f the active site heme and inhibits iNOS and neuronal NOS (nNOS) by preventing the formation of enzym

21 nhibition of eNOS, inducible NOS (iNOS), and neuronal NOS (nNOS) activities.

22 ribution of both macrophage NOS (macNOS) and neuronal NOS (nNOS) immunoreactivity in normal and infla

23 We therefore examined, in normal and neuronal NOS (nNOS)-deficient mice, the influence of pO2

24 itu, whereas inhibition of inducible NOS and neuronal NOS had no effect.

25 endothelial nitric oxide synthase (NOS) and neuronal NOS in adult transgenic myocytes, which constit

26 Inducible NOS (iNOS or NOS2) and neuronal NOS (nNOS or NOS1) proteins were not detected a

27 PCoA superoxide-mediated vasoreactivity and neuronal NOS-mediated production of NO decreased with ag

28 ia-sensitive subcortex of wild-type (Wt) and neuronal NOS (nNOS) and endothelial NOS (eNOS)-deficient

29 by the oxygenase domains of endothelial and neuronal NOSs (eNOSoxy and nNOSoxy).

30 abeling of cardiac SR vesicles by using anti-neuronal NOS (nNOS), but not anti-endothelial NOS (eNOS)

31 n cultured RG2 cells, but not as strongly as neuronal NOS expression.

32 Sarcolemma-associated neuronal NOS (nNOS) plays a critical role in normal musc

33 ide synthase (eNOS-/-) and mice lacking both neuronal NOS (nNOS) and eNOS (nNOS-/-, eNOS-/-) have a t

34 The kinetics of CO ligation with both neuronal NOS and its heme domain module were determined

35 over Arg hydroxylation reaction catalyzed by neuronal NOS to document the process, determine its kine

36 rebral circulation, whereas that produced by neuronal NOS (nNOS) participates in the regulation of br

37 al whether nitric oxide (NO.) synthesized by neuronal NOS (nNOS) plays an excitatory or inhibitory ro

38 y of the brains tested and that constitutive neuronal NOS activity was similar across the two hemisph

39 In contrast, neuronal NOS (NOS1)-deficient mice and wild-type mice tr

40 sion of a constitutive Ca(2+) /CaM-dependent neuronal NOS in the central and peripheral nervous syste

41 forms of NOS, namely endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS) in cardiov

42 xists in 3 isoforms: endothelial NOS (eNOS), neuronal NOS (nNOS), and inducible NOS (iNOS).

43 e other nitric-oxide synthase (NOS) enzymes, neuronal NOS (nNOS) turnover and activity are regulated

44 e other nitric-oxide synthase (NOS) enzymes, neuronal NOS (nNOS) turnover and activity are regulated

45 ubset of GABAergic interneurons that express neuronal NOS (nNOS).

46 ration in cultured neuronal cells expressing neuronal NOS (nNOS).

47 and BH(4) to the dimeric, BH(4)-free ferric neuronal NOS (NNOS) oxygenase domain expressed in Escher

48 NADPH-diaphorase (a commonly used marker for neuronal NOS activity) positive neurons in specific hypo

49 yl)- and N(5)-(1-iminohexyl)-l-ornithine for neuronal NOS (1.7, 3, 20, >1,900 microM, respectively) a

50 showed the cis-isomers to be more potent for neuronal NOS and selective over endothelial NOS compared

51 Immunohistological staining for neuronal NOS (bNOS), combined with retrograde labeling o

52 cardiac mitochondria and it is derived from neuronal NOS (nNOS).

53 ult in >50% inhibition of NO generation from neuronal NOS.

54 This NO stems from neuronal NOS (nNOS), but not endothelial (eNOS).

55 removed the 33 and 42 residue C termini from neuronal NOS (nNOS) and endothelial NOS (eNOS), respecti

56 This article reviews how neuronal NOS (nNOS) and endothelial NOS (eNOS) knockout

57 Corresponding human neuronal NOS (nNOS) and murine inducible NOS (iNOS) fusi

58 In contrast, inhibition of human neuronal NOS and endothelial NOS (eNOS) was relatively w

59 drochloride (ARL 17477) on recombinant human neuronal NOS (nNOS) and endothelial NOS (eNOS).

60 e therefore measured changes in hypothalamic neuronal NOS (nNOS) in DIO and investigated effects of p

61 Here, we show that: (i) neuronal NOS has PQ diaphorase activity that inversely c

62 The nitric oxide synthases (NOS-I, neuronal, NOS-II, inducible, and NOS-III, endothelial) a

63 No immunoreactive neuronal NOS was detected.

64 observed no colocalization of immunoreactive neuronal NOS (nNOS) with CGRP in the dorsal horn.

65 These results implicate neuronal NOS in the N(2)O response.

66 In neuronal NOS (nNOS), protein domain dynamics and calmodu

67 module in electron transfer and catalysis in neuronal NOS.

68 ndrogen treatment was mimicked by changes in neuronal NOS mRNA level.

69 , spreading acidification could be evoked in neuronal NOS-deficient mice (B6;129S-Nos1(tm1plh)).

70 We mutated Phe-1395 (F1395) in neuronal NOS to Tyr and Ser and tested their effects on

71 We investigated its importance in neuronal NOS (nNOS) by mutating the two residues that pr

72 Modification of the small insertion in neuronal NOS tends to increase cytochrome c reduction bu

73 residue Gly-810 from the FMN binding loop in neuronal NOS (nNOS) to give Delta G810 so that the short

74 ate the function of one of these residues in neuronal NOS (nNOS), we generated and characterized muta

75 key tolerance-associated proteins, including neuronal NOS (nNOS), in dorsal horn.

76 siological effects associated with increased neuronal NOS (nNOS) or inducible NOS (iNOS) activity in

77 mulation of the nicotinic receptor increases neuronal NOS (nNOS) expression in cultured gastric myent

78 or afferent input, which revealed increasing neuronal NOS expression with age.

79 ress proteins that interact with and inhibit neuronal NOS and endothelial NOS, macrophage proteins th

80 of the nitric-oxide synthase (NOS) isoforms neuronal NOS, inducible NOS (iNOS), and endothelial NOS

81 three different NO synthase (NOS) isoforms: neuronal NOS (nNOS), endothelial NOS, and immunologic NO

82 de from L-arginine exists in three isoforms: neuronal NOS (nNOS), endothelial NOS (eNOS), and inducib

83 nduced radical intermediates in the isolated neuronal NOS oxygenase domain (nNOSox) have been similar

84 ctron transfer domains in a FRET dye-labeled neuronal NOS reductase domain, and to understand how cal

85 amine this concept, we utilized a "Cys-lite" neuronal NOS flavoprotein domain and substituted Cys for

86 ducing activity similar to that of mammalian neuronal NOS.

87 similar regulatory domains to the mammalian neuronal NOS (nNOS).

88 ated with cardiomyocyte caveolin-3, and more neuronal NOS (nNOS) translocation to caveolin-3 during i

89 iNOS and down-regulation of endothelial NOS, neuronal NOS, and VEGF, an effect that was restored by s

90 It is unknown whether endothelial NOS, neuronal NOS, or both caused the elevation of the NO end

91 corresponding residues 725-754 of rat NOS1 (neuronal NOS).

92 biting inducible NO synthase (NOS2), but not neuronal NOS (NOS1), partially restored the evoked secre

93 ible NO synthase and endothelial NOS but not neuronal NOS genes were expressed in urothelial cells.

94 endothelial NOS, and nitrotyrosine, but not neuronal NOS, were significantly elevated in the inflamm

95 a model in which rapid, brief activation of neuronal NOS initiates the erectile process, whereas PI3

96 participate in the biochemical activation of neuronal NOS.

97 se, and cytochrome c reductase activities of neuronal NOS and also altered heme reduction.

98 al neovascularization, whereas deficiency of neuronal NOS (nNOS) or inducible NOS (iNOS) suppresses c

99 nd determine the subcellular distribution of neuronal NOS (nNOS).

100 ized the heme-containing oxygenase domain of neuronal NOS (nNOSoxy) and stopped-flow methods to study

101 The reductase domain of neuronal NOS (nNOSr) contains a widely conserved acidic

102 ly, were replaced by the reductase domain of neuronal NOS.

103 To circumvent the effects of neuronal NOS (nNOS), DAF-2/DA was perfused in the presen

104 was determined by differential expression of neuronal NOS (nNOS) and postsynaptic PKC activity, both

105 The expression of neuronal NOS (nNOS) protein determined by Western blot w

106 to exercise in alpha2-KD mice; expression of neuronal NOS (NOSmicro) was also reduced.

107 d cell death through increased expression of neuronal NOS and iNOS but not endothelial NOS.

108 lonic inflammation induces the expression of neuronal NOS in the spinal cord and that increased produ

109 The expression of neuronal NOS or protein levels at 2, 4 and 8 hours post-

110 High levels of expression of neuronal NOS were detected in cultured and intracerebral

111 tron transfer into and out of the flavins of neuronal NOS in the calmodulin-free state, and is also r

112 athways are proposed for the inactivation of neuronal NOS (nNOS) by (S)-2-amino-5-(2-(methylthio)acet

113 s linked to nerve growth factor induction of neuronal NOS (nNOS).

114 Pharmacological selective inhibition of neuronal NOS (nNOS) has the potential to be therapeutica

115 nts in an AD model through the inhibition of neuronal NOS (nNOS)- and non-NOS-dependent components of

116 en tested with selective local inhibition of neuronal NOS (nNOS).

117 Inhibition of neuronal NOS or inducible NOS did not affect baselines,

118 highly selective compounds for inhibition of neuronal NOS over the other isozymes.

119 nhibitor (L-NNA), or a specific inhibitor of neuronal NOS (7-NI).

120 08 or 7-nitroindazole (7-NI, an inhibitor of neuronal NOS).

121 logues as potent and selective inhibitors of neuronal NOS (nNOS).

122 logues as potent and selective inhibitors of neuronal NOS (nNOS).

123 chain as potent and selective inhibitors of neuronal NOS.

124 tic (VA) halothane on the enzyme kinetics of neuronal NOS derived from different regions of the rat c

125 urons in the rat that contain high levels of neuronal NOS (nNOS) for the presence of the NMDAR1 recep

126 The localization of neuronal NOS (nNOS) at the plasma membrane of muscle has

127 the corresponding W678A and W678F mutants of neuronal NOS.

128 onsiderably increases the turnover number of neuronal NOS (nNOS).

129 accompanied by an increase in the number of neuronal NOS+ cells determined immunohistochemically, wh

130 pression or stabilization, and the number of neuronal NOS+ neurons.

131 To study specifically the role of neuronal NOS (nNOS), MPTP was administered to mutant mic

132 geminate combination and partial trapping of neuronal NOS (nNOS) through a futile regenerating pathwa

133 soform of NOS whilst having little effect on neuronal NOS reactivity.

134 changes in O2 concentration have effects on neuronal NOS enzymatic activity and gene expression that

135 ronal MA concentrations and their effects on neuronal NOS function and excitotoxic injury.

136 ber of cells labeled for NADPH diaphorase or neuronal NOS in the lumbosacral spinal cord after intrac

137 lineages, whereas endothelial NOS (eNOS) or neuronal NOS (nNOS) mutant mice showed comparable T(H)17

138 ibition at low dose Iso, and by preferential neuronal NOS inhibition at high-dose Iso.

139 motor neuron survival in part by preventing neuronal NOS expression.

140 directly measure NO formation from purified neuronal NOS.

141 his possibility rigorously, we expressed rat neuronal NOS (nNOS) in Escherichia coli, with the homolo

142 d the role of the FMN-FAD/NADPH hinge in rat neuronal NOS (nNOS) by constructing mutants that either

143 mational equilibria of the FMN module in rat neuronal NOS (nNOS).

144 mation of the docked FMN/heme complex in rat neuronal NOS.

145 omains in truncated oxyFMN constructs of rat neuronal NOS (nNOS) and murine inducible NOS (iNOS), in

146 us-O2 complex of the oxygenase domain of rat neuronal NOS (nNOS) by bubbling O2 through a solution of

147 truncated two-domain oxyFMN construct of rat neuronal NOS (nNOS), in which only the FMN and heme doma

148 Arg1229 of rat neuronal NOS is a conserved residue in the FAD domain th

149 ormants were generated by overexpressing rat neuronal NOS in HEK 293T cells.

150 However, recombinant neuronal NOS-derived peptides from spiked mitochondrial

151 alternative splicing specifically regulates neuronal NOS (nNOS, type I) in striated muscle.

152 x NOS activity despite a decrease in remnant neuronal NOS abundance.

153 ical in gp120-mediated damage in the retina, neuronal NOS-deficient [nNOS(-/-)], endothelial NOS-defi

154 s, each with a different physiological role: neuronal NOS, endothelial NOS, and inducible NOS (iNOS).

155 activity correlates with loss of sarcolemmal neuronal NOS localization in mdx muscle, whereas loss of

156 mg/kg of 7-nitroindazole (7-NI), a selective neuronal NOS inhibitor, or equal volume of vehicle (dime

157 reported tetrahydroquinoline-based selective neuronal NOS inhibitors due to higher lipophilicity.

158 r (100-800 nmol) as well as by the selective neuronal NOS inhibitor ARL 17477 (30-600 nmol).

159 al and renal NO synthase (NOS), specifically neuronal NOS.

160 (NOS) activity (NADPH-diaphorase staining), neuronal NOS (nNOS) protein, and nNOS mRNA were assessed

161 on of a fully assembled, electron-supplying, neuronal NOS reductase dimer.

162 d lower cytochrome c reductase activity than neuronal NOS (nNOS), implying significantly different el

163 It is concluded that neuronal NOS expression in the myenteric plexus is indep

164 Interestingly, experiments confirmed that neuronal NOS was activated in response to calcium-permea

165 We hypothesized that neuronal NOS (nNOS, NOS I) effects cutaneous vasodilatat

166 on in cardiac myocytes, we hypothesized that neuronal NOS (NOS1) found in cardiac sarcoplasmic reticu

167 Western blot analysis revealed that neuronal NOS (nNOS) but not endothelial NOS (eNOS) prote

168 The neuronal NOS was located in the adventitia of P and NP a

169 ster (L-NAME), but not its d-isomer, and the neuronal NOS (nNOS) inhibitor 7-nitroindazole completely

170 s active as the inducible NOS (iNOS) and the neuronal NOS (nNOS), respectively.

171 sponding loop in cytochrome P450 BM3 and the neuronal NOS mutant (DeltaGly-810).

172 Recombinant adenovirus (Ad) carrying the neuronal NOS gene (nNOS) targeted liver sinusoidal endot

173 y CRISPR/Cas9-mediated knockout of nNOS (the neuronal NOS isoform) in mGCH1-Tg.

174 The crystal structure of the neuronal NOS (nNOS) connecting/FAD binding subdomains re

175 AG decreased MAP, while the injection of the neuronal NOS (nNOS) inhibitor, 1-(2-trifluoromethylpheny

176 A comparison of the structure of the neuronal NOS FAD/NADPH domain and CYPOR reveals the stri

177 a beta hairpin in structural studies of the neuronal NOS reductase domains adjacent to the calmoduli

178 Signaling via the neuronal NOS (nNOS) splice variant nNOSmu is essential f

179 her divided into: (a) those treated with the neuronal NOS (nNOS) inhibitor, 7-nitroindazole (7-NI), f

180 ARTp-IR fibers exhibited immunoreactivity to neuronal NOS (a marker for nitric oxide-producing neuron

181 RPC6-IR fibers exhibited immunoreactivity to neuronal NOS.

182 endothelial nitric oxide synthase (NOS)- to neuronal NOS-mediated vasorelaxation, as well as alterat

183 ation under single-turnover conditions using neuronal NOS (nNOS), whose heme iron reduction requires

184 To determine whether neuronal NOS (nNOS) and endothelial NOS (eNOS) are criti

185 However, it is not known whether neuronal NOS (nNOS)-derived NO regulates tissue hyperaem

186 h human MC lines and skin-derived MCs, while neuronal NOS (nNOS) was variably expressed in the MC pop

187 y (ka congruent with 2 x 10(7) M-1 s-1) with neuronal NOS in both its ferric and ferrous oxidation st

188 Here, we present additional experiments with neuronal NOS (nNOS) and inducible NOS (iNOS) variants (n

189 GK was also found to interact stably with neuronal NOS as detected by coimmunoprecipitation and fl