ライフサイエンスコーパス: nitrite reductase

1 yi represents a new subclass of cytochrome c nitrite reductase.

2 llele encoding a highly efficient gonococcal nitrite reductase.

3 reductase, flavorubredoxin, or cytochrome c nitrite reductase.

4 ermolecular electron transfer from azurin to nitrite reductase.

5 y sluggishly as electron donors to the green nitrite reductase.

6 esemble those found in ascorbate oxidase and nitrite reductase.

7 rologously expressed Rhodobacter sphaeroides nitrite reductase.

8 that nitrite acts as a positive effector of nitrite reductase.

9 ype 1 and catalytic Type 2 copper centers in nitrite reductase.

10 ric oxide reductase, and one had nitrate and nitrite reductase.

11 ent, and activities of nitrate reductase and nitrite reductase.

12 a highly sensitive oxygen sensor and/or as a nitrite reductase.

13 previously been observed in any cytochrome c nitrite reductase.

14 , as a model for the Cu(T2) center of copper nitrite reductase.

15 ls of xanthine oxidoreductase (XOR), a known nitrite reductase.

16 pseudoazurin that is a donor of electrons to nitrite reductase.

17 XO and AO, but not hemoglobin, are critical nitrite reductases.

18 trification for certain bacterial and fungal nitrite reductases.

19 nate electrons to both blue and green copper nitrite reductases.

20 significant identity with other copper-type nitrite reductases.

21 rate is reduced with a unique set of nitrate-nitrite reductases.

22 ents a physiologically relevant new class of nitrite reductases.

23 only as a cofactor in assimilatory sulphite/nitrite reductases.

24 b1 and AHb2, respectively) might function as nitrite reductases.

25 ZmNLP5 directly regulates the expression of nitrite reductase 1.1 (ZmNIR1.1) by binding to the nitra

26 The cd(1) nitrite reductase, a key enzyme in bacterial denitrifica

27 The structure of nitrite reductase, a key enzyme in the process of nitrog

28 ide, which inhibits hemoglobin function as a nitrite reductase, abolishes nitrite bioactivation.

29 his nitrite may be reduced back to NO by the nitrite reductase action of deoxygenated hemoglobin, aci

30 ound haem, but the methyl-viologen-dependent nitrite reductase activities associated with the mutated

31 inding affinities, reduction potentials, and nitrite reductase activities of these copper-peptide com

32 als at a specific pH vary by 100 mV, and the nitrite reductase activities range over a factor of 4 in

33 flavoHb possesses nitrosoglutathione and nitrite reductase activities that may account for the pr

34 and can produce nitric oxide via nitrate and nitrite reductase activities.

35 ix, significantly increases copper-catalyzed nitrite reductase activity (CuNiR).

36 A strain exhibited a severe decrease in both nitrite reductase activity and expression of a nirK-lacZ

37 decreases E(1/2)), an effect that increases nitrite reductase activity and vasodilation at any given

38 hus allowing for the clear-cut monitoring of nitrite reductase activity as a function of quaternary s

39 n resulted in extracts possessing dithionite-nitrite reductase activity but no NADPH-nitrite reductas

40 gns include those with carbonic anhydrase or nitrite reductase activity by incorporating a ZnHis(3) o

41 Recent literature reports suggest a nitrite reductase activity for Hb, converting nitrite in

42 logous promoter, suggesting that the loss of nitrite reductase activity in the PrrA and PrrB mutants

43 Nitrite reductase activity in the PrrA strain could be r

44 This nitrite reductase activity is highly regulated as it is

45 s that can explain the origin of the reduced nitrite reductase activity of deoxyHbE and the accelerat

46 Nitrite reductase activity of deoxyhemoglobin (HbA) in t

47 and NO-modified hemoglobin resulted from the nitrite reductase activity of deoxyhemoglobin and deoxyg

48 ory for nitric oxide (NO), bioactivated by a nitrite reductase activity of deoxyhemoglobin.

49 Here, we characterize the nitrite reductase activity of deoxymyoglobin, which redu

50 ration and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under

51 The nitrite reductase activity of hemoglobin is modulated by

52 the hemoglobin tetramer, suggesting that the nitrite reductase activity of hemoglobin is under allost

53 tivirus expression systems, we show that the nitrite reductase activity of neuroglobin inhibits cellu

54 We evaluated the nitrite reductase activity of unpolymerized sickle hemog

55 cytochrome c reductase activity but no NADPH-nitrite reductase activity or dithionite-nitrite reducta

56 of our cupredoxin models, and enhancement of nitrite reductase activity up to 1000-fold.

57 Although nitrite reductase activity was not affected by the crp m

58 of oxygen limitation, nasDEF expression and nitrite reductase activity were significantly induced.

59 We now report that HbS exhibits allosteric nitrite reductase activity with competing proton and red

60 oxyhemoglobin, our findings suggest that the nitrite reductase activity within the circulation, under

61 al N accumulation and chlorophyll synthesis, nitrite reductase activity, and reduced AAO activity.

62 paz inactivation did not decrease nitrite reductase activity, but loss of pseudoazurin and

63 We also report that CblC exhibits nitrite reductase activity, converting cob(I)alamin and

64 (eNOS) inhibition had no effect on vascular nitrite reductase activity, in RBCs L-NAME, L-NMMA, and

65 d-type Type 1 center, and a somewhat reduced nitrite reductase activity.

66 e activity and an FAD-independent dithionite-nitrite reductase activity.

67 DPH-nitrite reductase activity or dithionite-nitrite reductase activity.

68 oli capable of yielding high levels of NADPH-nitrite reductase activity.

69 and CcoP established to be critical to NirK nitrite reductase activity.

70 unctional gene, nirS (encoding cytchrome-cd1 nitrite reductase), along the salinity gradient of San F

71 In copper nitrite reductases, an electron is delivered from the ty

72 hat are predicted to be monoheme and part of nitrite reductase and a bc1 complex using genome analyse

73 (ORFs), aniA and norB, predicted to encode a nitrite reductase and a nitric oxide reductase, respecti

74 are observed for single molecules of a blue nitrite reductase and are used to extract the microscopi

75 The newly described nitrite reductase and denitration activities of CblC ext

76 O up-regulates the expression of the plastid nitrite reductase and genes involved in the subsequent i

77 , including strains that are able to express nitrite reductase and grow in anaerobic environments, su

78 tive polymerase chain reaction targeting the nitrite reductases and dissimilatory sulfite reductase,

79 ced and lacks ammonium transporters, nitrate/nitrite reductases and glutamine:2-oxoglutarate aminotra

80 h the d1 -haem cofactor of dissimilatory cd1 nitrite reductases and haem, via the novel alternative-h

81 important implications for the evolution of nitrite reductases and multicopper oxidases.

82 of both the M. catarrhalis aniA (encoding a nitrite reductase) and norB (encoding a putative nitric

83 cluded those encoding a nitrate reductase, a nitrite reductase, and a nitric oxide reductase.

84 including nitrate transporters, nitrate and nitrite reductase, and metabolic enzymes such as transal

85 iated, respectively, with nitrate reductase, nitrite reductase, and nitric oxide reductase, which cat

86 ion (<1.20 A) structure of a copper protein, nitrite reductase, and of a mutant of the catalytically

87 icyanin, the type 1 copper-binding region of nitrite reductase, and the binuclear CuA binding region

88 ductase, NAD(P)-linked and copper-containing nitrite reductases, and a nitric oxide reductase are inv

89 Third, the reaction undergoes a nitrite reductase/anhydrase redox cycle that catalyzes t

90 d that M. catarrhalis cells that express the nitrite reductase (AniA protein) can produce NO. by redu

91 irects the expression of a copper-containing nitrite reductase, AniA, in response to the presence of

92 Copper nitrite reductases are categorised into two subgroups ba

93 Nitrite reductases are key enzymes that perform the firs

94 phic structures of several copper-containing nitrite reductases are now available.

95 nylglyoxal allowed the identification of two nitrite reductase arginines, R375 and R556, that are pro

96 carbonic anhydrase as a nitrous anhydrase or nitrite reductase as a mechanism for its inhibition of H

97 ls have previously been identified in copper nitrite reductases based on high- to atomic-resolution c

98 Heme-containing nitrite reductases bind and activate nitrite by a mechan

99 ion of N(2)O by both intact chloroplasts and nitrite reductase, but not by nitrate reductase, indicat

100 number of nirK gene copies, that encodes for nitrite reductase by 40.9% and increased the number of n

101 sibility of the active site pocket of copper nitrite reductase by mutation of a surface-exposed pheny

102 catalysed by the cytoplasmic, NADH-dependent nitrite reductase by the double mutant was almost as rap

103 Cytochrome cd1 nitrite reductase catalyses the conversion of nitrite to

104 Dissimilatory nitrite reductase catalyses the reduction of nitrite (NO

105 Nitrite reductase catalyzes the reduction of nitrite to

106 Cytochrome c nitrite reductase (ccNiR) is a periplasmic, decaheme hom

107 f NO2(-) reduction by multiheme cytochrome c nitrite reductase (ccNIR) leads us to propose that NO2(-

108 Cytochrome c nitrite reductases (ccNiRs) catalyze reduction of nitrit

109 tween the enzyme that produces NO; the cd(1) nitrite reductase (cd(1)NiR) and the enzyme that reduces

110 A model for the ferredoxin:nitrite reductase complex is proposed in which the bindi

111 We found that the robust activity of the nitrite reductase complex NirBD depended on expression o

112 Copper-containing nitrite reductases contain both type 1 and type 2 Cu sit

113 Nitrite reductase contains an unusual blue-green Type 1

114 enigmatic di-iron enzyme ScdA functions as a nitrite reductase, converting nitrite to nitric oxide (N

115 Like their nitrite reductase counterparts, sulfite reductases requi

116 fer between the copper centers in the copper nitrite reductase (CuNiR) family of enzymes.

117 ormed by either a haem- or copper-containing nitrite reductase (CuNiR) where they receive an electron

118 nitrifier dentrification pathway is a copper nitrite reductase (CuNIR).

119 genase (PHM) or a catalytic center in copper nitrite reductase (CuNiR).

120 Copper nitrite reductases (CuNIRs) convert NO(2)(-) to NO as we

121 Copper nitrite reductases (CuNiRs) exhibit a strong pH dependen

122 Copper-containing nitrite reductases (CuNiRs), encoded by nirK gene, are f

123 ossess atypical heme-binding sites, the NrfA nitrite reductase (CXXCK) and the SirA sulfite reductase

124 The inability of microaerobically grown nitrite reductase-deficient cells to induce nirK-lacZ ex

125 K-lacZ expression was severely impaired in a nitrite reductase-deficient strain of 2.4.3.

126 membrane nitrate reductase (Delta narGH) and nitrite reductase (Delta nirS) produced defects in the e

127 n the (Fe4S4)-siroheme-binding domain of the nitrite reductase demonstrated that these residues were

128 tecture of blue copper oxidase is similar to nitrite reductases, detailed structural alignments show

129 In addition, N. crassa nitrite reductase displays several partial activities in

130 irm that rusticyanin is in the same class as nitrite reductase domain 2, laccase domain 3 and cerulop

131 ganisms with copper containing dissimilatory nitrite reductases, electron donation from a reduced cup

132 ted herein, a single NADH-dependent, soluble nitrite reductase encoded by the nasDE genes is required

133 Neurospora crassa NAD(P)H-nitrite reductase, encoded by the nit-6 gene, is a solub

134 Both membrane nitrate reductase and nitrite reductase enzyme complexes were important for cy

135 Escherichia coli possesses two distinct nitrite reductase enzymes encoded by the nrfA and nirB o

136 Previously characterized nitrite reductases fall into three classes: siroheme-con

137 e, which need not be the case in the sulfite/nitrite reductase family.

138 pology was observed for ferredoxin-dependent nitrite reductase (Fd-NiR), indicating the genes encodin

139 eral ferredoxin-interacting proteins, namely nitrite reductase, Fd:NADP+ oxidoreductase, and Fd:thior

140 ing nitrate transporters, nitrate reductase, nitrite reductase, ferredoxin reductase, and enzymes in

141 t these positions in all of the Fd-dependent nitrite reductase for which sequences are available, sug

142 expression system for the copper-containing nitrite reductase from a denitrifying variant of Rhodoba

143 the gene nirK, which encodes the copper-type nitrite reductase from a denitrifying variant of Rhodoba

144 e-soaked oxidized and nitrite-soaked reduced nitrite reductase from Alcaligenes faecalis have been de

145 Crystallographic structures of the blue nitrite reductase from Alcaligenes xylosoxidans (AxNiR)

146 ues has been investigated in the blue copper nitrite reductase from Alcaligenes xylosoxidans (NCIMB 1

147 PFV) has been used to study the cytochrome c nitrite reductase from Escherichia coli (ecNrfA) previou

148 e newly characterized three-domain haem-c-Cu nitrite reductase from Ralstonia pickettii (RpNiR) at 1.

149 ype 2 (T2) copper site at high and low pH in nitrite reductase from Rhodobacter sphaeroides.

150 which could correspond to NO location in the nitrite-reductase function of Ngb.

151 In contrast, one nitrite reductase functions both in nitrite nitrogen ass

152 cular, we characterized the diversity of the nitrite reductase gene (aniA), the factor H-binding prot

153 or 19S promoters and PTGS on the endogenous nitrite reductase gene (Nii).

154 n studies revealed a differential pattern of nitrite reductase gene expression where optimal nrfA-lac

155 Strongly elevated expression of nitrite reductase genes compared to nitrous oxide reduct

156 Expression of nitrate and nitrite reductase genes was also significantly enhanced

157 comparison of plastocyanin (blue), wild-type nitrite reductase (green), and the Met182Thr mutant (blu

158 g in heme proteins such as those observed in nitrite reductase, guanylyl cyclase, and possibly cytoch

159 The blue-green Type 1 center of nitrite reductase has a redox, electron-transfer role, a

160 periments, it has been suggested that copper nitrite reductases have specific electron donors and tha

161 has identity to a motif in the gene encoding nitrite reductase in strain 2.4.3 is critical for nor op

162 anthine oxidoreductase (XOR) is an important nitrite reductase in the heart and kidney.

163 function as either a nitrous anhydrase or a nitrite reductase in the lungs of pigs, and probably oth

164 ded nitrite enhanced the in vivo activity of nitrite reductase in the narB mutant; this suggests that

165 utant (Met182Thr) of Rhodobacter sphaeroides nitrite reductase in which the axial methionine has been

166 ribe properties of a penta-heme cytochrome c nitrite reductase in which the distal His has been subst

167 d(1), the prosthetic group of dissimilatory nitrite reductases in anaerobic, denitryfying bacteria.

168 meproteins have been reported to function as nitrite reductases in mammalian cells, it is unknown whe

169 also xanthine oxidoreductase, a nitrate and nitrite reductase, in cornea and sclera.

170 ession of nirK, the structural gene encoding nitrite reductase, in these strains was significantly de

171 ytic efficiency ( k(cat)/ K(M)) of native Cu nitrite reductase involve both substrate binding ( K(M))

172 Cytochrome c nitrite reductase is a dimeric decaheme-containing enzym

173 mined whether the primary human erythrocytic nitrite reductase is hemoglobin as opposed to other eryt

174 cyanide for reduced versus oxidized forms of nitrite reductase is immediately revealed, as is the pre

175 In contrast, the nirB nitrite reductase is optimally synthesized only when nit

176 odes an anaerobically induced outer membrane nitrite reductase) is necessary for expression.

177 ded beta-propeller domain of cytochrome cd1 (nitrite reductase) is seen, from a 1.28 A resolution str

178 association with the physiological partner, nitrite reductase, is only affected by the Met16Phe muta

179 e expression of the gene aniA, which encodes nitrite reductase, is regulated by oxygen depletion and

180 Siroheme, the cofactor for sulfite and nitrite reductases, is formed by methylation, oxidation,

181 gh AO has structural similarity to bacterial nitrite reductases, it is unknown whether AO-catalyzed n

182 Faster nitrite reductase kinetics for CDB3-bound hemoglobin sug

183 vidence of adaptation, with the assimilatory nitrite reductase (nasD) and urease (ureG) showing the h

184 ubtilis, NsrR represses transcription of the nitrite reductase (nasDEF) genes that are under positive

185 genes (NOL2 and NOL3) and nitrate (NIA1) and nitrite reductase (NII1), generating single and double D

186 he role of NO3- and NO2- in the induction of nitrite reductase (NiR) activity in detached leaves of 8

187 d to both nitric oxide dioxygenase (NOD) and nitrite reductase (NiR) activity.

188 NO is produced by nitrite reductase (Nir) and reduced to nitrous oxide by

189 nder anaerobic conditions, copper-containing nitrite reductase (NiR) of Rhodobacter sphaeroides yield

190 anaerobically by anaerobic respiration using nitrite reductase (Nir) to convert nitrite to NO and nit

191 Banding patterns of nitrate reductase (NR), nitrite reductase (NiR), and glutamine synthetase (GS) f

192 inetics of a copper-containing dissimilatory nitrite reductase (NiR).

193 doreductases nitrite oxidoreductase (NxrAB), nitrite reductase (NirK), ammonia monooxygenase (AmoABC)

194 e reductase (nirS) over the copper-dependent nitrite reductase (nirK).

195 oxidation, formate dehydrogenase (FDH) and a nitrite reductase (NirK).

196 These data suggest that NO generated via nitrite reductase NirS contributes to the regulation of

197 the essential cofactor of the cytochrome cd1 nitrite reductase NirS.

198 Analysis of expressed genes for nitrite reductase (nirS) and a nitrogenase subunit (nifH

199 ce of genes encoding for the iron-containing nitrite reductase (nirS) over the copper-dependent nitri

200 es: the mononuclear type two (T2) Cu site in nitrite reductases (NiRs) and the trinuclear Cu cluster

201 Heme nitrite reductases (NiRs) are key enzymes in the assimil

202 Copper-containing nitrite reductases (NiRs) are sub-divided into blue and

203 absorption spectral changes present in green nitrite reductases (NiRs) due to a thermodynamic equilib

204 from nitrite by heme- and copper-containing nitrite reductases (NIRs).

205 and low N(2)O yield due to a small ratio of nitrite reductase: nitrous oxide reductase in these rive

206 inhibition, whereas all but the periplasmic nitrite reductase NrfA provide protection against neutro

207 ransfer from NrfH, the electron donor to the nitrite reductase NrfA.

208 ite as a terminal electron acceptor requires nitrite reductase (nrfA) as a D. vulgaris nrfA mutant ca

209 Cytochrome c nitrite reductase (NrfA) catalyzes the reduction of nitr

210 lso affect NarP-dependent gene regulation of nitrite reductase (nrfABCDEFG) and aeg-46.5 gene express

211 electrochemical response of the cytochrome c nitrite reductase of Desulfovibrio desulfuricans.

212 ctron-transfer kinetics to the physiological nitrite reductase of Pseudomonas.

213 The nitrate and nitrite reductases of Bacillus subtilis have two differe

214 tron donation experiments using three copper nitrite reductases, one green and two blue, and five cup

215 deoxyhemoglobin is the primary erythrocytic nitrite reductase operating under physiological conditio

216 duced during nitrate respiration include the nitrite reductase operon (nasDEF) and the flavohemoglobi

217 erent expression profile for the alternative nitrite reductase operon encoded by nirBDC under high-ni

218 nite-nitrite reductase activity but no NADPH-nitrite reductase or NADPH-cytochrome c reductase activi

219 Nitric oxide (NO), produced by nitrite reductases or nitric oxide synthases, performs v

220 ytic cycle for C-H amination inspired by the nitrite reductase pathway.

221 Cytochrome c nitrite reductases perform a key step in the biogeochemi

222 w physiological function for hemoglobin as a nitrite reductase, potentially contributing to hypoxic v

223 y the addition of a 131-bp fragment from the nitrite reductase promoter.

224 n in resistance artery endothelium acts as a nitrite reductase providing local nitric oxide in respon

225 , including nrfA, which encodes cytochrome c nitrite reductase, providing strong evidence that there

226 The nitrite reductase reaction does not appear to account fu

227 rite to generate nitric oxide (NO) through a nitrite reductase reaction.

228 the presence of a reductant, evidencing the nitrite reductase reactivity for FeS clusters.

229 HbA) through several reactions including the nitrite reductase, reductive nitrosylation, and still co

230 Formate-dependent nitrite reductase regulator (FNR) is an oxygen-sensitive

231 roxylamine reductase (PG0893) and a putative nitrite reductase-related protein (PG2213) in P. gingiva

232 trate, is a more efficient electron donor to nitrite reductase relative to Fd.

233 rite to ammonium by assimilatory nitrate and nitrite reductase, respectively.

234 of the genes encoding nitrate reductase and nitrite reductase, resulting in strains that were unable

235 ferredoxin to a positively charged region of nitrite reductase results in elimination of exposure of

236 The structure of nitrite reductase shows a broad similarity to the hemopr

237 ry nitrate reductase) and nasF (required for nitrite reductase siroheme cofactor formation), constitu

238 Shewanella oneidensis cytochrome c nitrite reductase (soNrfA), a dimeric enzyme that houses

239 The implications of the nitrite reductase structure for understanding multi-elec

240 onditions of hypoxia and in the presence of "nitrite reductases" such as heme- and molybdenum-contain

241 at hemoglobin is an allosterically regulated nitrite reductase, such that oxygen binding increases th

242 f bacteria that have an assimilatory nitrate/nitrite reductase system (NAS) can use nitrate or nitrit

243 ll living systems as part of the sulfite and nitrite reductase systems.

244 he nir operon encodes a soluble, cytoplasmic nitrite reductase that catalyses NADH-dependent reductio

245 r and a post-translationally redox-regulated nitrite reductase that generates NO under six-to-five-co

246 or hemoglobin as an allosterically regulated nitrite reductase that may mediate nitric oxide (NO)-dep

247 est an additional role for cytochrome c as a nitrite reductase that may play an important role in reg

248 unctional analysis of T1Cu centre mutants of nitrite reductase that perturb the redox potential and t

249 lobin A (HbA) is an allosterically regulated nitrite reductase that reduces nitrite to NO under physi

250 Thus, YtfE is a high-affinity, low-capacity nitrite reductase that we propose functions to relieve n

251 cofactor in a conserved class of sulfite and nitrite reductases that catalyze the six-electron reduct

252 G is coregulated with the siroheme-dependent nitrite reductase, the cysF gene is regulated by sulfur

253 a coli K-12 nrf operon encodes a periplasmic nitrite reductase, the expression of which is driven fro

254 ion, which affects the inducible cytoplasmic nitrite reductase, the major source of nitric oxide duri

255 Transcription of genes encoding nitrate and nitrite reductases, the enzymes responsible for NO produ

256 Only a single isozyme of nitrite reductase was detected in surveys of three tetra

257 The gene encoding nitrite reductase was not found in 2.4.1.

258 cea oleracea) leaf ferredoxin (Fd)-dependent nitrite reductase was treated with either the arginine-m

259 15 degreesC, although nitrate reductase and nitrite reductase were still active.

260 uctase A and the cytoplasmic, NADH-dependent nitrite reductase were unaffected by the narK and narU m

261 some nitrogen acquisition transcripts (e.g. nitrite reductase) were upregulated at depth compared wi

262 istidine and 3-methyl-histidine to create Cu nitrite reductases where delta- or epsilon-nitrogen liga

263 rall, HAO is seen to act like a cytochrome c nitrite reductase, which catalyzes the six-electron redu

264 itate the bifunctional nature of copper-type nitrite reductase, which could easily remove NO(2)(-) vi

265 bin as an allosterically and redox-regulated nitrite reductase whose "enzyme activity" couples hypoxi

266 Modification of nitrite reductase with pyridoxal-5'-phosphate, followed

267 nce that Hb exhibits enzymatic behavior as a nitrite reductase, with maximal NO generation rates occu

268 e present the structures of wild-type copper nitrite reductase (wtNiR) from Alcaligenes xylosoxidans