ライフサイエンスコーパス: nitrosylate

1 ysteine antibody indicated that PARP-1 was S-nitrosylated.

2 e zinc-tetrathiolate cysteines of eNOS are S-nitrosylated.

3 the majority of mitochondrial caspase-9 is S-nitrosylated.

4 lated to produce NO, the exogenous tTG was S-nitrosylated.

5 revealed that E1A and E7 are both protein-S-nitrosylated.

6 nes of TGFbeta1 which were predicted to be S-nitrosylated.

7 of exogenous reagents (three of 21) or as S-nitrosylated.

8 mplex formation was impeded when actin was S-nitrosylated.

9 re, we show that PSD-95 is physiologically S-nitrosylated.

10 tension (pO(2) approximately 10 mmHg), NO S-nitrosylates 1 of approximately 50 free cysteines per ry

11 pO2, nanomolar NO activates the channel by S-nitrosylating a single cysteine residue.

12 nson's or Alzheimer's disease, that PDI is S-nitrosylated, a reaction transferring a nitric oxide (NO

13 ntry and, thus, the spread of infection by S-nitrosylating ACE2 via targeted delivery of the drug aft

14 Prior work has shown that hyperoxia causes S-nitrosylated actin (SNO-actin) formation, which mediates

15 ectrospray-ionization mass spectrometry, and nitrosylated actin was glutathiolated by reduced GSH.

16 nted polymerization normally observed with S-nitrosylated actin, VASP binding to actin, elevated Rac

17 ) regulates cellular function, in part, by S-nitrosylating active site thiol groups of proteins.

18 ced cell death and causes the release of non-nitrosylated (active) DAMPs that induce a potent antimel

19 heterologously expressed human DDAH II was S-nitrosylated after cytokine induced expression of the in

20 composites were perfused with/without the S-nitrosylating agent ethyl nitrite (ENO) present in the p

21 e treated whole-heart homogenates with the S-nitrosylating agent S-nitrosoglutathione and determined

22 Interestingly, the NO donor and nitrosylating agent SNAP reversed the depressed RyR2 ope

23 We reasoned that addition of an S-nitrosylating agent to the preservation solution could c

24 imicked by S-nitroso-glutathione (GSNO; an S-nitrosylating agent).

25 tive care or care augmented by 20 ppm of the nitrosylating agent, ethyl nitrite, blended into the ven

26 scle cells by S-nitrosocysteine (CSNO), an S-nitrosylating agent, in human umbilical vein endothelial

27 We suggest that a nitrosylating agent, possibly derived from HNO, is produ

28 Irrespective of the type of S-nitrosylating agent, S-nitrosylated proteins formed upon

29 Exogenous S-nitrosylating agents are in various stages of clinical d

30 cule S-nitrosoglutathione (GSNO) and other S-nitrosylating agents can cause full maturation of the ab

31 cum) Bright Yellow-2 cells, an increase in S-nitrosylating agents occurred.

32 ata suggest that GSNO is one of a class of S-nitrosylating agents that act independently of the class

33 ort that 1) GSNO is only one of a class of S-nitrosylating agents that, at low micromolar concentrati

34 ney 293 cells treated with S-oxidizing and S-nitrosylating agents, and verify that the assay is highl

35 gher oxides, such as N(2)O(3), were actually nitrosylating agents.

36 panied by increased nitrotyrosine formation, nitrosylated alpha-synuclein, and microglial activation.

37 -permeable S-nitrosothiols and that sGC is S-nitrosylated and desensitized in the tolerant, treated t

38 asis, which were reflected by increases in S-nitrosylated and nitrated proteins in the lungs from inf

39 YtfE exists in a mixture of forms, including nitrosylated and nitrite-bound, when isolated from nitri

40 Thus, DR4 residue C336 becomes S nitrosylated and promotes apoptosis following NO-Cbl tre

41 f the HR, the bacterial effector HopAI1 is S-nitrosylated and that this modification inhibits its pho

42 Consequently, nitrosylated and thereby inactivated caspase 3 or cathep

43 Ca(2+), up to 15 cysteines were found to be nitrosylated and this modification resulted in an inhibi

44 fore, we investigated whether Panx1 can be S-nitrosylated and whether this modification can affect ch

45 findings demonstrate that iNOS-derived NO S-nitrosylates and activates cPLA(2)alpha in human cells.

46 domain, binds COX-2 with the generated NO S-nitrosylating and activating the enzyme.

47 is progressively PKA-hyperphosphorylated, S-nitrosylated, and depleted of the phosphodiesterase PDE4

48 Here we show that cPLA(2)alpha protein is S-nitrosylated, and its activity is enhanced by nitric oxi

49 RyR2s are oxidized, nitrosylated, and PKA hyperphosphorylated, resulting in

50 These findings suggest that S-nitrosylated arginase1 can compete with NOS for L-argini

51 in GPCR trafficking, interacts with and is S-nitrosylated at a single cysteine by endothelial NO synt

52 amin, which interacts with NO synthase, is S-nitrosylated at a single cysteine residue (C607) after s

53 r by iNOS induction caused GAPDH to become S-nitrosylated at Cys152.

54 As a result, the HIF-1alpha protein is S-nitrosylated at Cys533 (through "biotin switch" assay) i

55 ) release NO when hemoglobin that has been S-nitrosylated at Cys93 of the beta-chain (betaCys93) tran

56 g cells, a subset of caspase-3 zymogens is S-nitrosylated at the active site cysteine, inhibiting enz

57 ing H2O2 levels in plants, was found to be S-nitrosylated at the onset of both PCDs.

58 Low micromolar levels of S-nitrosylated bovine serum albumin (BSA), but not control

59 The generated NO, in turn, nitrosylates BVR, leading to nuclear translocation where

60 Also, p50 was S-nitrosylated by DETANONOate resulting in inhibition of N

61 ies also indicate that Ras Cys(118) can be S-nitrosylated by direct reaction of Cys(118) with a gluta

62 d from our studies suggest that Ras can be S-nitrosylated by direct reaction of Cys(118) with nitroge

63 We report that NSF is physiologically S-nitrosylated by endogenous, neuronally derived nitric ox

64 A family of proteins S-nitrosylated by iNOS-S100A8/A9 were revealed by proteomi

65 tein when they are one-electron oxidized, or nitrosylated by nitric oxide.

66 e and sarcosine dehydrogenase (SarDH), are S-nitrosylated by NO under strictly anaerobic conditions.

67 In both cells and tissues, GRK2 is S-nitrosylated by SNOs as well as by NO synthases, and GRK

68 Here we show that C. difficile toxins are S-nitrosylated by the infected host and that S-nitrosylati

69 Tissue TG can be poly-S-nitrosylated by the NO carrier, S-nitrosocysteine (CysNO

70 Increased levels of S-nitrosylated C3 were present in female over male AD brai

71 ally, the factors that instill Cu(II) with S-nitrosylating capabilities and modulate activity are not

72 s the binding affinity of calstabin-1 to the nitrosylated channel, inhibited sarcoplasmic reticulum C

73 lt of the conformational change induced by S-nitrosylated CLIC4 that leads to unfolding of the protei

74 thermodynamically controls reactivity toward nitrosylated compounds.

75 PDZ-containing nNOS (nNOSalpha) increases S-nitrosylated CREB with consequent augmented binding on c

76 chment protein receptor (SNARE) complexes by nitrosylating critical cysteine residues of NSF.

77 Previous studies suggested that S-nitrosylated Cys may be flanked by an acid-base motif or

78 c approaches that are capable of identifying nitrosylated Cys residues have been developed.

79 NO S-nitrosylates Cys77 in human pVHL (cognate to mouse Cys43

80 ng proteomic strategy, we identified 1,276 S-nitrosylated cysteine residues [S-nitrosothiol (SNO)] on

81 to ultraviolet light which photo-reverses S-nitrosylated cysteine residues and by co-incubations wit

82 s exposed to UV light, which photoreverses S-nitrosylated cysteine residues and by co-incubations wit

83 Structural analyses revealed that S-nitrosylated cysteine residues were equally distributed

84 Seventy percent of the S-nitrosylated cysteine residues were surrounded by negati

85 the gasotransmitter nitric oxide directly S-nitrosylates cysteine residues in diverse intracellular

86 Two potential S-nitrosylated cysteines in the alpha- and beta-subunits o

87 ield a mixture of penta- and hexa-coordinate nitrosylated cyt c.

88 In the nitrosylated cyt c.CL complex, NO chemically reacted wit

89 cell lymphoma/leukemia (Bcl)-2 or Bcl-X(L), nitrosylated cytochrome c is found in the mitochondria.

90 Nitrosylated cytochrome c is found predominantly in the

91 ylhaemoglobin in the 'tense' T (or partially nitrosylated, deoxy) structure to S-nitrosohaemoglobin i

92 For example, tegument protein pp71 is nitrosylated, diminishing its ability to inhibit STING,

93 but unlike RyR1, RyR2 was not activated or S-nitrosylated directly by NO.

94 and subjected to the biotin switch assay; S-nitrosylated DR4 was detected in all three cell lines.

95 ion of a mutant form of HDAC2 that cannot be nitrosylated dramatically inhibits Brm expression.

96 effect by NO was reversible since, after the nitrosylated enzyme was reduced with DTT followed by inc

97 Other lipid mediators such as nitrosylated-fatty acids and omega-3 fatty acid-derived

98 e(NO)(2) (S(5) )](-) is a simple synthon for nitrosylated Fe-S clusters via its reduction with PPh(3)

99 2L/TRAIL death receptor DR4 (TRAIL R1) was S nitrosylated following NO-Cbl treatment.

100 The less active, nitrosylated form of NAB1 is found in cells acclimated t

101 SP-D, including significant amounts of an S-nitrosylated form.

102 Specifically, we show that DJ-1 is S-nitrosylated (forming SNO-DJ-1); subsequently, the NO gr

103 Here we report that Prx2 is S-nitrosylated (forming SNO-Prx2) by reaction with nitric

104 ic oxide, its ferredoxin [2Fe-2S] cluster is nitrosylated, forming the dinitrosyl iron complex with a

105 he BST using differentially S-oxidized and S-nitrosylated forms of protein tyrosine phosphatase 1B, a

106 esent study, we tested whether NO directly S-nitrosylates (forms an NO-adduct at a cysteine residue)

107 Levels of nitrosylated GAPDH and nitrosylated HDAC2 were increased

108 Nitrosylated GAPDH complexes with the ubiquitin-E3-ligas

109 y cholate, and blocking nuclear transport of nitrosylated GAPDH reduced cholate-induced nitrosylation

110 GSH fully converted nitrosylated GAPDH to the reduced, active enzyme, withou

111 BDNF activates neuronal NOS with the nitrosylated GAPDH/seven in absentia (Siah) homolog comp

112 activate nitric oxide (NO) synthases with NO nitrosylating GAPDH, conferring on it the ability to bin

113 This is mediated by S-nitrosylated-GAPDH, which simultaneously inactivates Sir

114 A 2-fold increase in S-nitrosylated GCK was also observed in mouse islets.

115 alyzing TrxR1-dependent denitrosylation of S-nitrosylated glutathione or of HEK293 cell-derived S-nit

116 l responses of Escherichia coli treated with nitrosylated glutathione or the nitric oxide (NO)-genera

117 adequate for the separation of glutathione, nitrosylated glutathione, and glutathione disulfide solu

118 tes generation of nitric oxide (NO), which S-nitrosylates glyceraldehyde-3-phosphate dehydrogenase (G

119 s variations in the contributions from fully nitrosylated Hb and oxidized Hb (MetHb).

120 erive from the functional behaviour of fully nitrosylated Hb, whereas Hb is only partially nitrosylat

121 Levels of nitrosylated GAPDH and nitrosylated HDAC2 were increased in cholestatic human a

122 eme iron was enhanced, and the EPR signal of nitrosylated heme iron became discernible.

123 om dinitrosyl complexes of non-heme iron and nitrosylated heme iron in both K/VP.5-iNOS cells and K/V

124 , S-nitrosothiols, N-nitros-amines, and iron-nitrosylated heme proteins within 1-30 minutes of reperf

125 reasing the yield of S-nitrosohemoglobin and nitrosylated hemes.

126 S-nitrosylated hemoglobin (SNO-Hb) within red blood cells

127 hway for the formation of beta-93 cysteine S-nitrosylated hemoglobin [SNOHb:S-nitrosohemoglobin], whi

128 ted with rapid formation of erythrocyte iron-nitrosylated hemoglobin and, to a lesser extent, S-nitro

129 The levels of nitrosylated hemoglobin are too low to affect overall he

130 udy reveal a previously unexplored role of S-nitrosylated hemoglobin in cardioprotection.

131 NO inhalation markedly raised total nitrosylated hemoglobin levels, with a significant arter

132 baseline, the arterial and venous levels of nitrosylated hemoglobin were not significantly different

133 ion led to a dose-dependent increase in mean nitrosylated hemoglobin, and at the highest dosage, a si

134 vities and in assays of endogenous Fe- and S-nitrosylated hemoglobin.

135 reaction is immediately autocaptured to form nitrosylated hemoglobin.

136 obin's vacant hemes in a cooperative manner, nitrosylates hemoglobin thiols, or reacts with liberated

137 We further show that S-nitrosylated HIF-1alpha binds to the vascular endothelia

138 In contexts where pp71 is not protein S-nitrosylated, host antiviral response was inhibited.

139 evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidase, sugges

140 that protein disulfide isomerase (PDI) is S-nitrosylated in brains of patients with sporadic neurode

141 ilar NO dioxygenase activities in vitro, are nitrosylated in Cys residues in vivo, and scavenge NO in

142 ently reported that eNOS is constitutively S-nitrosylated in endothelial cells and that agonists prom

143 involved in contraction and relaxation are S-nitrosylated in laboring and nonlaboring muscle and that

144 te protein (ALG-1)-at a site conserved and S-nitrosylated in mammalian Argonaute 2 (AGO2)-alters its

145 ggest that during apoptosis, cytochrome c is nitrosylated in mitochondria and then rapidly released i

146 d that many of these proteins are uniquely S-nitrosylated in only one state of the tissue.

147 We now show that sGC can be S-nitrosylated in primary aortic smooth muscle cells by S-

148 inducible nitric oxide synthase (iNOS) and S-nitrosylated in proinflammatory peritoneal macrophages.

149 We report that eNOS is tonically S-nitrosylated in resting bovine aortic endothelial cells

150 tTG nitrosylated in the absence of Ca(2+) was 6-fold more su

151 recently shown that p65 is constitutively S-nitrosylated in the lung and that LPS-induced injury eli

152 namic cycle exists in which haemoglobin is S-nitrosylated in the lung when red blood cells are oxygen

153 Remarkably, SRG3 but not SRG2 was S-nitrosylated in vitro and in vivo.

154 We show that parkin is S-nitrosylated in vitro, as well as in vivo in a mouse mod

155 itrosylated Hb, whereas Hb is only partially nitrosylated in vivo.

156 ntains approximately 84 free thiols and is S-nitrosylated in vivo.

157 urthermore, we identified that gephyrin is S-nitrosylated in vivo.

158 This indicates that NO readily nitrosylates intracellular free heme and prevents its de

159 ive stress, releasing nitric oxide (NO) from nitrosylated iron, and reducing NO to nitrous oxide (N(2

160 ative of mechanisms that actively dispose of nitrosylated iron-sulfur centers.

161 r DNICs account for only a minor fraction of nitrosylated iron.

162 that iNOS specifically binds to COX-2 and S-nitrosylates it, enhancing COX-2 catalytic activity.

163 that nitric oxide (NO) reacts with XIAP by S-nitrosylating its RING domain (forming SNO-XIAP), thereb

164 addition, treatment with VEGF-A decreased S-nitrosylated laminin in cultured podocytes.

165 fraction of the spectral signature of fully nitrosylated (largely hexacoordinate) Hb disappears as t

166 We show that SR is physiologically S-nitrosylated leading to marked inhibition of enzyme acti

167 n of Cys-819 allow Cys-110 to be oxidized or nitrosylated, leading to complete inactivation of IDE.

168 The metal-free N-nitrosylated ligand (DAC-NO + H)+ has two conformations:

169 = 1,4,8,11-tetraazacyclotetradecane), the N-nitrosylated ligand DAC-NO, and the Roussin's red salt e

170 we report that E1A and E7 are both protein-S-nitrosylated like pp71.

171 systematically investigate oxidized and/or S-nitrosylated mitochondrial proteins and to use a biotin-

172 markedly increased numbers of oxidized and S-nitrosylated mitochondrial proteins following hepatic I/

173 The oxidized and/or S-nitrosylated mitochondrial proteins from I/R-injured mou

174 biotin-N-maleimide-labeled oxidized and/or S-nitrosylated mitochondrial proteins from pair-fed contro

175 S-nitroso-CoA (SNO-CoA) as its cofactor to S-nitrosylate multiple proteins (SNO-CoA-assisted nitrosyl

176 sing synthesis of nitric oxide (NO), which S-nitrosylated N-ethylmaleimide sensitive factor (NSF), a

177 eNOS) production of nitric oxide (NO), which nitrosylates N-ethylmaleimide sensitive factor (NSF) and

178 Recently, a new class of nitrosylated NSAID (known as NO-NSAIDs) has been develop

179 O increases S-nitrosylated NSF levels, but S-nitrosylated NSF levels decrease within 3 h after exposu

180 Endogenously synthesized NO increases S-nitrosylated NSF levels, but S-nitrosylated NSF levels d

181 Knockdown of TRX1 increases the level of S-nitrosylated NSF, prolongs the inhibition of exocytosis,

182 d NSF, and endogenous TRX1 removes NO from S-nitrosylated NSF.

183 dm1a, a well-known epigenetic modifier, is S-nitrosylated on Cys334.

184 dition, the class I member HDAC2 was found S-nitrosylated on cysteine, a post-transduction modificati

185 Here we demonstrate that cytochrome c is nitrosylated on its heme iron during apoptosis.

186 Furthermore, we show that BVR is S-nitrosylated on one of three cysteines and that this pos

187 Caspase-3 zymogens were found to be S-nitrosylated on their catalytic-site cysteine in unstimu

188 Finally S-nitrosylated ornithine decarboxylase was isolated from i

189 was protein kinase A-hyperphosphorylated, S-nitrosylated, oxidized, and depleted of its stabilizing

190 Further, an increase in S-nitrosylated p50 was detected in cells, and the level wa

191 gene transcription, and nuclear levels of S-nitrosylated p65 correlate with decreased DNA binding of

192 We demonstrate that COP1 is S-nitrosylated, particularly under shade.

193 einyl affinity resin to selectively enrich S-nitrosylated peptides reduced by ascorbate followed by n

194 Upon M.tb infection, secreted S-nitrosylated PknG phosphorylates macrophage Silencer of

195 A subset of proteins are constitutively S-nitrosylated, playing roles in the regulation of tissue

196 These thiols may be nitrosylated preferentially during increasing hypoxia or

197 that inhibition of the denitrosylation of S-nitrosylated procaspase-3 mediated by the redox protein

198 on/NO scavenging, we show that the amount of nitrosylated products formed greatly exceeds what can be

199 Levels of S-nitrosylated protein are controlled in part by the denit

200 e, eliminated the light-evoked increase in S-nitrosylated protein immunofluorescence (SNI) in the ret

201 channel alpha1 subunit as the predominant S-nitrosylated protein in membrane fractions, and followin

202 y and improved survival rates by restoring S-nitrosylated protein levels in the brain after CA/CPR in

203 t, liver, spleen, and plasma, and restored S-nitrosylated protein levels in the brain.

204 .05 vs. VEH) and increased nitrite and total nitrosylated protein levels.

205 P activity colocalized with immunoreactive S-nitrosylated protein.

206 Here, we discuss how aberrantly S-nitrosylated proteins (SNO-proteins) play a crucial role

207 Steady-state levels of S-nitrosylated proteins are largely determined by denitros

208 aced on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidati

209 We identified several candidate S-nitrosylated proteins by proteomic analysis following th

210 S-nitrosoglutathione or denitrosylation of S-nitrosylated proteins by reduced GSH.

211 increase of up to 11-fold in the number of S-nitrosylated proteins during regeneration.

212 tive of the type of S-nitrosylating agent, S-nitrosylated proteins formed upon exposure to both of th

213 Only a few intracellular S-nitrosylated proteins have been identified, and it is un

214 09 SNO sites, representing a wide range of S-nitrosylated proteins in 40 postmortem AD and non-AD hum

215 sing a proteomic approach we characterized S-nitrosylated proteins in citrus leaves exposed to chemic

216 We identify endogenously S-nitrosylated proteins in subcellular organelles, includi

217 n prevented the reduction in the number of S-nitrosylated proteins in the brain, mitigated brain inju

218 activated GSNOR and reduced the number of S-nitrosylated proteins in the brain.

219 An unbiased proteomic approach to identify S-nitrosylated proteins in valve cells found enrichment of

220 Functional analysis of differentially S-nitrosylated proteins indicated their involvement in apo

221 Determination of S-nitrosylated proteins is of great importance for fundame

222 AC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates

223 The overall patterns of oxidized and/or S-nitrosylated proteins resolved by 2-dimensional polyacry

224 cal outcome, GSNOR activity, and levels of S-nitrosylated proteins were evaluated.

225 In addition, S-nitrosylated proteins were quantitated using the fluorom

226 e challenge, characterized by the accrual of nitrosylated proteins without a major alteration in cell

227 roteomic identification of over a thousand S-nitrosylated proteins, few S-nitrosylases have been iden

228 modified biotin switch method to identify S-nitrosylated proteins.

229 rols intracellular levels of both GSNO and S-nitrosylated proteins.

230 free nitric oxide increases the frequency of nitrosylated proteins.

231 oups, including many previously unrevealed S-nitrosylated proteins.

232 associated with alterations in the nuclear S-nitrosylated proteome.

233 Accordingly, by decreasing S-nitrosylated Prx2 (SNO-Prx2), overexpression of Srxn1 pr

234 S-Nitrosylated Ras (Ras-SNO) can be formed when NO serves

235 fector of Ras), or GNE rates relative to non-nitrosylated Ras.

236 athways affected by iNOS indicated that NO S-nitrosylated Ras.

237 ithiothreitol, which triggers reduction of S-nitrosylated residues.

238 ted by protein kinase A (PKA), oxidized, and nitrosylated, resulting in depletion of the stabilizing

239 neurons that stargazin is physiologically S-nitrosylated, resulting in increased surface expression.

240 ivation increases iNOS generation of NO to S-nitrosylate RING1A, a key member of the polycomb repress

241 We report two crystal structures of nitrosylated RuBisCO from the red algae Galdieria sulphu

242 led intracellular Ca2+ leak via oxidized and nitrosylated RyR2 channels, activated ER stress response

243 ble guanylyl cyclase to form cGMP, NO also S-nitrosylates selected protein targets.

244 RyR1 macromolecular complex was oxidized, S-nitrosylated, Ser-2844 phosphorylated, and depleted of t

245 After photo-excitation of nitrosylated sGC, only NO geminate rebinding occurs in 7

246 protein (VASP) exhibits high affinity for S-nitrosylated short filamentous actin, which increases ac

247 rt that in neurons and brain tissue NO can S-nitrosylate SHP-2 at its active site cysteine, forming S

248 SHP-2 at its active site cysteine, forming S-nitrosylated SHP-2 (SNO-SHP-2).

249 the NO donor compound S-nitrosoglutathione S-nitrosylates significantly more proteins in mitochondria

250 tates identification and quantification of S-nitrosylated sites by mass spectrometry.

251 These are 408 S-nitrosylated sites in 360 protein groups showing an accu

252 e ease with which it can detect individual S-nitrosylated (SNO) proteins in biological samples.

253 Purified, nitrosylated SoxR has transcriptional activity similar t

254 In contrast, nitrosylated SoxR is short-lived in intact cells, indica

255 By native PAGE, formation of S-nitrosylated SP-D in vivo resulted in disruption of SP-D

256 recruitment of effector cells modulated by S-nitrosylated SP-D.

257 the decreased intensities of EPR signals of nitrosylated species.

258 from properties of the target protein, the S-nitrosylating species, or both.

259 rmation feeds back to presynaptic cells to S-nitrosylate SR and decrease D-serine availability to pos

260 inescence in side-by-side assays of multiple nitrosylated standards of varied reactivities and in ass

261 x that can exist in an oxidized, reduced, or nitrosylated state.

262 ith the Cys(85) thiol group in reduced and S-nitrosylated states.

263 The cellular content of S-nitrosylated Syntaxin 4 peaked acutely, within 5 min of

264 treatments, including well known and novel S-nitrosylated targets.

265 whereas specific, high affinity binding of S-nitrosylated TG2 (SNO-TG2) to endothelial surfaces resto

266 We demonstrate that nitric oxide (NO) can S-nitrosylate the ER stress sensors IRE1alpha and PERK.

267 , consistent with the known ability of NO to nitrosylate the Fe(II) center in Fur.

268 NO S-nitrosylates the cysteine residue in the active site of

269 Nitric oxide nitrosylates the WhiB1 iron-sulfur cluster and promotes

270 edback inhibits iNOS gene transcription by S-nitrosylating the trans-activator PARP-1 and decreasing

271 itric oxide (NO) and NO-related species that nitrosylate thiols.

272 set of mitochondrial proteins that can be S-nitrosylated through multiple reaction channels, includi

273 n suggested that multiple cysteines may be S-nitrosylated to regulate Panx1 channel function.

274 This was associated with the appearance of S-nitrosylated TonEBP/NFAT5, as monitored by the biotin-sw

275 Furthermore, S-nitrosylated tTG inhibited platelet aggregation induced

276 The addition of Ca(2+) to nitrosylated tTG was able to trigger the release of NO g

277 f myeloperoxidase as well as chlorinated and nitrosylated tyrosine epitopes in their lesion areas com

278 Nitrosylated tyrosines, a stable marker for NO generatio

279 Up to 20% of myoglobin can be nitrosylated under gastro-intestinal conditions in this

280 revealed more than 300 proteins that were S-nitrosylated upon illumination, many of which are known

281 Furthermore, S-nitrosylated USP9X was shown to deubiquitinate and stabi

282 that encode a pp65 that cannot be protein-S-nitrosylated, we evaluated the impact of this PTM on vir

283 While wild-type eNOS is robustly S-nitrosylated, we found that S-nitrosylation of the Myr-

284 ino acids we identified in pp71 as protein S-nitrosylated, we found this pp71 PTM diminishes its abil

285 To identify which cysteine residue(s) was S-nitrosylated, we made single cysteine-to-alanine substit

286 e site Cys(453) determined by isolation of S-nitrosylated wild type but not active site Cys(453) -->

287 bsence of Ca(2+), up to eight cysteines were nitrosylated without modifying TGase activity.

288 on of nitrite to di-ferrous YtfE resulted in nitrosylated YtfE and the release of NO.