ライフサイエンスコーパス: nonglycosylated

1 ying an MSG gene lacking the UCS expressed a nonglycosylated 130-kDa protein.

2 ycosylated 91-kDa subunit, gp91(phox), and a nonglycosylated 22-kDa subunit, p22(phox).

3 ain protein detected on Western blots as the nonglycosylated 27-kDa form, with overexpression of this

4 e mutant forms of FR-beta were identified as nonglycosylated (29.3 kDa) or glycosylated polypeptides

5 CD20 is a nonglycosylated 33 to 37 kD phosphoprotein involved in B

6 AgSF2 represents a nonglycosylated 40-kDa protein that has novel internal a

7 The nonglycosylated 44-kDa P2X(4) receptor was resistant to

8 In this study, we investigated a unique nonglycosylated active fragment of hepatocyte growth fac

9 dimers in aldimine intermolecular linkage to nonglycosylated alpha1(I) K930.

10 that the enhanced epimerization relative to nonglycosylated amino acids is due to a combination of f

11 MR structural characterizations of refolded, nonglycosylated amino-terminal fragments, and another pa

12 ) GalNAc(alpha-->)Thr10 contulakin-G and its nonglycosylated analog were prepared using an Fmoc (9-fl

13 n carcinomas as a combination of both 27-kDa nonglycosylated and 28-kDa glycosylated mature two-chain

14 to determine the solution structures of both nonglycosylated and fucosylated FVII EGF-1 in the presen

15 of the properties of binding of IL-7 to both nonglycosylated and glycosylated forms of the IL-7Ralpha

16 halpy and entropy changes of IL-7 binding to nonglycosylated and glycosylated IL-7Ralpha.

17 44, whereas intracellular LOXL2 (~75-kDa) is nonglycosylated and N-terminally processed, and is prima

18 ecombinant glycosylated full-length Ags, and nonglycosylated and truncated Ags recognized by 4E10 usi

19 ut homology to mammalian sequences labeled a nonglycosylated approximately 41-kDa protein (spx1) on W

20 e capacity of recombinant, histidine-tagged, nonglycosylated BclA (rBclA) given with adjuvant to prot

21 YFP-tagged, nonglycosylated beta2 displayed mobility kinetics in the

22 We found that fully nonglycosylated beta2 was mostly retained in the endopla

23 Two nonglycosylated BiP substrates, NS-1kappa light chain an

24 To investigate the disposal of nonglycosylated BiP substrates, we used a nonsecreted ka

25 coproteins > neoglycoproteins based on BSA > nonglycosylated BSA >> free sugars.

26 ate that enhanced binding of 3-6-fold over a nonglycosylated CBM is achieved by the addition of a sin

27 increasing it up to 140-fold relative to the nonglycosylated CBM.

28 x activity was modulated by competition with nonglycosylated ceramides.

29 Because nonglycosylated channels appear to pass through the secr

30 using purified collagen type I, type IV, and nonglycosylated, commercially available recombinant SLRP

31 ble entropy changes and smaller unfavorable (nonglycosylated complex) and favorable (glycosylated com

32 N- or C-terminal activities relative to the nonglycosylated control peptides.

33 ented N-linked glycosylation, resulting in a nonglycosylated core protein devoid of ATP and ADP hydro

34 ylated antibody populations outcompete their nonglycosylated counterparts for the available RBD molec

35 of COX-2 protein, which is distinct from the nonglycosylated COX-2 protein generated in the presence

36 e in the E2P form in complex with bufalin (a nonglycosylated CTS with a six-membered lactone) and dig

37 ions (a) a newly synthesized, highly mobile, nonglycosylated, cytosolic receptor that is depleted by

38 Highly methylated, nonglycosylated derivatives of the flavonoid myricetin f

39 re stimulated with peptides representing the nonglycosylated domain of G protein.

40 1A domain (GA733 type 2 motif), and a unique nonglycosylated domain without cysteines (GA733 type 3 m

41 es with and promotes degradation of nascent, nonglycosylated Eag1 proteins at the ER.

42 mature core-glycosylated, as well as nascent nonglycosylated, Eag1 proteins.

43 It is a nonglycosylated envelope protein that is regulated as a

44 1 (HSV-1) UL20 gene encodes a 222-amino-acid nonglycosylated envelope protein which forms a complex w

45 hybridomas specific for the glycosylated and nonglycosylated epitope CII(259-273) were developed.

46 osylated EPO had a 20-fold slower k(on) than nonglycosylated EPO, indicating that the core carbohydra

47 ed for ubiquitination or degradation of this nonglycosylated ERAD substrate.

48 the present report, folding comparisons of a nonglycosylated erythropoietin (EPO) mutant are made wit

49 Nonglycosylated erythropoietin bearing acetamidomethyl p

50 HSV-1 infectivity.IMPORTANCE HSV-1 UL20 is a nonglycosylated essential envelope protein that is highl

51 show that glycosylated ETSHR-gp, but not the nonglycosylated ETSHR protein, can react with autoantibo

52 Earlier, we identified two nonglycosylated exofacial regions of erythrocyte band 3

53 Two nonglycosylated exofacial regions of human band 3 in the

54 in bacterial biofilm formation, whereas the nonglycosylated Fap1 peptide mediates bacterial initial

55 IL-8 release from A549 cells stimulated with nonglycosylated flagellar preparations (having less then

56 When the inhibitor and its nonglycosylated form (SmCI N23A mutant) were overproduce

57 newly synthesized Aaop1 is converted to the nonglycosylated form and deposited in the rhabdomeres.

58 elevated concentrations of AVP (100 nM), the nonglycosylated form desensitized to the same extent as

59 wever, the delta Cj1496c mutant expressing a nonglycosylated form of Cj1496c exhibited levels of inva

60 ivity was observed in strains expressing the nonglycosylated form of EmaA.

61 The ability of TgI4.4 to recognize a nonglycosylated form of gI suggests that HSV-1 recogniti

62 deficiency virus (HIV)-1 gp120 and env2-3, a nonglycosylated form of gp120.

63 PDGFRalpha did exhibit binding activity to a nonglycosylated form of PDGFRalpha.

64 ingently regulated than is processing of the nonglycosylated form of the same epitope.

65 uired more extensive processing than did the nonglycosylated form of the same epitope.

66 of the spike is considerably reduced in its nonglycosylated form, confining the area explored by the

67 es: a slow migrating glycosylated form and a nonglycosylated form.

68 The proteins are found in glycosylated and nonglycosylated forms in infected cells.

69 Both glycosylated and nonglycosylated forms of Drm exhibit identical distribut

70 ons bearing combinations of glycosylated and nonglycosylated forms of prM and E could infect mammalia

71 in the steady-state levels of the mono- and nonglycosylated forms of PrP in the brain.

72 V2) from potato (Solanum tuberosum) than its nonglycosylated forms.

73 Here we report that nonglycosylated full-length and C-terminal truncated amy

74 The in vitro fucosylation of the nonglycosylated FVII EGF-1 was achieved by using O-fucos

75 Nonglycosylated G protein also interacted with CNX, but

76 If, however, unfolded nonglycosylated G protein was the starting material, ref

77 A structure of nonglycosylated G(C) reveals an extended conformation th

78 cific hydrophobic amino acid residues on the nonglycosylated GFCC'C" face of CEACAM1 N-terminal domai

79 ubstrate specificity of the glycosylated and nonglycosylated glycoforms.

80 sing the local maximum at 100 mug/mL for the nonglycosylated/glycosylated heavy chain pair is recomme

81 ein, with high affinity, but it did not bind nonglycosylated gp120 or BSA.

82 its subunits, including the addition of the nonglycosylated heavy chain.

83 It was found to be a nonglycosylated heterodimeric protein, belonging to the

84 eta glycosylation inhibition revealed 30-47% nonglycosylated hFSHbeta associated with four of six hFS

85 Nonglycosylated HIV envelope protein was not associated

86 We also show that nonglycosylated HIV-1(SF-2) gp120 or sodium metaperiodat

87 ation by tunicamycin treatment resulted in a nonglycosylated hOAT4, which was unable to target to the

88 bles into homo and hetero complexes with its nonglycosylated homologue, Rom-1, to maintain the integr

89 covalent homo- and hetero-tetramers with its nonglycosylated homologue, Rom-1.

90 centration of tunicamycin yielded completely nonglycosylated hPTH/PTHrP receptor (approximately 60 kD

91 The nonglycosylated hPTH/PTHrP receptor remains fully functi

92 gh molecular weight (approximately 190 kDa), nonglycosylated hUG-binding protein, readily detectable

93 ycosylated, indicating that glycosylated and nonglycosylated human calreticulin have indistinguishabl

94 Production of two recombinant nonglycosylated human serum transferrins (hTF-NG), conta

95 eir sensitivities to neighboring proline and nonglycosylated hydroxyamino acid residues, from which a

96 fter ATG5 knockdown, while expression of the nonglycosylated IE62 tegument protein was unchanged.

97 y size is sufficient to accommodate a single nonglycosylated Ig domain such as the CD3epsilon ectodom

98 High levels of nonglycosylated IgA were found in patients with Wiskott-

99 The results suggest that the unbound nonglycosylated IL-7Ralpha samples an extensive conforma

100 (1) approximately 10(2) M(-1) s(-1)) for the nonglycosylated interaction to a "diffusion-controlled"

101 d here suggests that the decreased number of nonglycosylated K(2P)3.1 channels on the cell surface ma

102 The nonglycosylated Kv1.2 protein had a similar protein half

103 It is a non-ATP-binding nonglycosylated ligand-regulated molecular chaperone of

104 phate (Man-6-P) modified proteins as well as nonglycosylated ligands.

105 and decreased cellulose interaction for the nonglycosylated linker.

106 RBD-ACE2, finding that both glycosylated and nonglycosylated mAb populations bound to RBD with high e

107 7/IL-24 signal peptide linked to the mutated nonglycosylated mda-7/IL-24 gene retained the ability to

108 Vaccination with the nonglycosylated, milk-derived MSP1(42) successfully prot

109 tant thyroglobulin, whereas degradation of a nonglycosylated misfolded secretory protein substrate ap

110 expressed MUC1 have not been successful when nonglycosylated MUC1 sequences were used for vaccination

111 s studies have localized these properties to nonglycosylated mucin domains, and we have recently show

112 ype vesicular stomatitis virus G protein and nonglycosylated mutant forms in the presence of microsom

113 In serial propagation with a nonglycosylated mutant PrP(C) substrate, the dominant Pr

114 allergens of Phl p 4 were expressed, and the nonglycosylated mutant was crystallized.

115 rsor of asialoglycoprotein receptor H2a, its nonglycosylated mutant, makes use of calnexin, EDEM1, an

116 ction, here we expressed the protein and its nonglycosylated N127Q mutant in HEK293F cells.

117 trioxacarcins appear to be derivatives of a nonglycosylated natural product known as DC-45-A2.

118 lecular mass (approximately 90-110 kDa) than nonglycosylated NIS (approximately 50 kDa).

119 ilar affinities; however, the double mutant, nonglycosylated NK1R showed only half the B(max) of the

120 sing a variety of antibodies, reveal a novel nonglycosylated nucleoporin, Nup84, that is tightly asso

121 e ON from E. coli and mammalian cell-derived nonglycosylated ON bind identically to type V collagen a

122 ly, the AGSs of the other DLBCLs were either nonglycosylated or occupied by complex-type glycans.

123 the peptide backbone specific for either the nonglycosylated or the glycosylated peptide.

124 gococci by a novel target recognition of two nonglycosylated outer membrane proteins, Opa and PorB.

125 ced at the cell surface, indicating that the nonglycosylated P2X2 receptors are retained inside the c

126 tion of a collagen triple helix, whereas the nonglycosylated peptide does not.

127 Nonglycosylated peptide fragments were susceptible to co

128 ated vaccine gave CTLs which recognized only nonglycosylated peptide.

129 e reactive with antibody than the synthetic (nonglycosylated) peptide, and periodate treatment of the

130 al sequences of many of the glycosylated and nonglycosylated peptides combined with published informa

131 Nonglycosylated peptides incidentally collected in the s

132 the column and separated from the remaining nonglycosylated peptides present in the digest.

133 Further quantitation of nonglycosylated peptides revealed that sialylation is in

134 copeptides from mixtures of glycosylated and nonglycosylated peptides, sequence both glycan and pepti

135 CTLs, which recognized both glycosylated and nonglycosylated peptides, whereas a similar nonglycosyla

136 ctural similarities between glycosylated and nonglycosylated peptides.

137 cosylation sites on-resin after synthesis of nonglycosylated peptides.

138 Furthermore, the nonglycosylated phytocyanin-like domain from GhPLA1, whi

139 rmed that the expression of glycosylated and nonglycosylated polypeptides occurred in appropriate sub

140 hydroceramide, sphingosine, only ceramide, a nonglycosylated precursor metabolite unable to bind to G

141 The retrotranslocation kinetics of nonglycosylated pro-alpha factor were not significantly

142 o antagonizes the proteasomal degradation of nonglycosylated pro-alpha factor, a postulated nonubiqui

143 HF were incubated with or without exogenous nonglycosylated proBNP1-108 tagged with 6 C-terminal His

144 major component is the 37-kDa, palmitylated, nonglycosylated product of the F13L gene.

145 Furthermore, only low amounts of the nonglycosylated products of the GPEET and EP2 genes were

146 had no effect on the rate of secretion of a nonglycosylated protein (human growth hormone [hGH]) tha

147 ted GLAST increased with maturation, whereas nonglycosylated protein decreased in abundance postnatal

148 We also find that OS-9 binds to a misfolded nonglycosylated protein destined for ERAD, but not to th

149 The native 17-kDa nonglycosylated protein inhibits both human and guinea p

150 Cell-free translation produced a nonglycosylated protein migrating at 29 kDa.

151 vel beta(2)-microglobulin-independent 37-kDa nonglycosylated protein on intestinal epithelia.

152 ted in activated T cells and gives rise to a nonglycosylated protein that resides in the cytosol.

153 his study, we showed that this intracellular nonglycosylated protein was as effective as wild-type MD

154 Sf9 system, homogeneous, crystallizable, and nonglycosylated protein was expressed after mutagenizing

155 h structure by 3.1 kcal/mol, relative to the nonglycosylated protein.

156 its dimeric form, and Epl1 is produced as a nonglycosylated protein.

157 Here, we studied three nonglycosylated proteins and analyzed their use of known

158 somal inhibition induced accumulation of the nonglycosylated proteins and ERAD machinery in the endop

159 Calreticulin also inhibits precipitation of nonglycosylated proteins and thus contains generic prote

160 Mechanisms of targeting of nonglycosylated proteins are less clear.

161 t a shared ERAD pathway for glycosylated and nonglycosylated proteins composed of luminal lectin mach

162 measurements indicate that glycosylated and nonglycosylated proteins induce "open" and "closed" P-do

163 how that calreticulin binds glycosylated and nonglycosylated proteins with similar affinities but dis

164 the IR spectra of six glycoproteins and two nonglycosylated proteins with the results from intact ma

165 regation and degradation of glycosylated and nonglycosylated proteins, and antibodies under various c

166 The primary membrane contains nonglycosylated proteins, appears as crescents in the cy

167 side of its mannosidase-like domain with the nonglycosylated proteins.

168 virtually identical with those observed with nonglycosylated pseudocathepsin D.

169 Using glycosylated and nonglycosylated purified flagellin and a cell reporter s

170 asigin, it was demonstrated that the native, nonglycosylated rBSG protein forms dimers in solution.

171 he band representing the photo-cross-linked, nonglycosylated receptor (obtained from the tunicamycin-

172 n enter the (a) "nuclear pathway," where the nonglycosylated receptor is extruded from the pre-Golgi

173 y for human IL-7 300-fold versus that of the nonglycosylated receptor through an allosteric mechanism

174 roduced on expression in transfected cells a nonglycosylated receptor with ligand binding affinity an

175 In contrast, the nonglycosylated receptor, in which all four glycosylatio

176 photoaffinity cross-link specifically to the nonglycosylated receptor.

177 The nonglycosylated receptors displayed normal function and

178 rescence revealed that the expression of the nonglycosylated receptors produced by either tunicamycin

179 lse-positive test results up to 2 kUA/L with nonglycosylated recombinant allergens in patients with h

180 Two forms of nonglycosylated recombinant alpha2AP were expressed, iso

181 A nonglycosylated recombinant ETSHR protein expressed earl

182 Because nonglycosylated recombinant FGF-5 does not appear to be

183 The production of a nonglycosylated recombinant form of Amb a 11 in Escheric

184 Natively glycosylated and nonglycosylated recombinant forms, lenograstim and filgr

185 Glycosylated and nonglycosylated recombinant Phl p 4 showed identical inh

186 M but only weakly inhibiting hydrolysis of a nonglycosylated recombinant protein substrate.

187 ly, these were all confined to two conserved nonglycosylated regions toward the carboxyl terminus of

188 NinaA associates with nonglycosylated Rh1(N20I); therefore, retention of nongl

189 thesized, glycosylated rhodopsin from mature nonglycosylated rhodopsin to characterize the fate of Aa

190 cosylated Rh1(N20I); therefore, retention of nonglycosylated rhodopsin within the ER is not due to th

191 levated concentration of ERManI, whereas the nonglycosylated secretory glycoprotein albumin was not a

192 tional high sensitivities to the presence of nonglycosylated Ser/Thr residues.

193 imultaneous analysis of the glycosylated and nonglycosylated species, we interrogate the hormone bios

194 omomultimers reside in core-glycosylated and nonglycosylated states.

195 culture medium ( approximately 40 mg/L), no nonglycosylated sTFR could be produced, suggesting that

196 of a glycosylated subunit, gp91(phox), and a nonglycosylated subunit, p22(phox).

197 is consistent with a failure of less stable, nonglycosylated subunits to replenish surface compartmen

198 nged in the mutant, suggesting that residual nonglycosylated surface hNETs execute a key step in the

199 ne regions that are related to transporters, nonglycosylated SVOP and highly glycosylated SV2, sugges

200 nt gp36 reduced the antibody reactivity, and nonglycosylated synthetic peptide repeat units from E. c

201 fic B*3501-restricted CTL clone recognized a nonglycosylated, synthetic nonamer representing the mini

202 -restricted, env peptide is identical with a nonglycosylated, synthetic nonamer.

203 to be active at 10-fold lower doses than the nonglycosylated Thr10 contulakin-G analog.

204 e binding affinities of contulakin-G and the nonglycosylated Thr10 contulakin-G for a number of neuro

205 The binding affinity of the nonglycosylated Thr10 contulakin-G was approximately an

206 However, both contulakin-G and nonglycosylated Thr10 contulakin-G were found to be pote

207 ted (N78 and not N30) (TIMP-1(glyc0/1)), and nonglycosylated (TIMP-1(glyc0/0)) TIMP-1, TIMP-1(glyc1/1

208 dly, Grp170 also promotes degradation of the nonglycosylated transthyretin (TTR) D18G misfolded clien

209 -type NIS activity, including the completely nonglycosylated triple mutant.

210 The expressed protein is nonglycosylated, unlike the protein isolated from the or

211 The nonglycosylated UT-A1 showed reduced localization in lip

212 Wild-type and nonglycosylated V2R yielded tritium signals at 45-55 and

213 nonglycosylated peptides, whereas a similar nonglycosylated vaccine gave CTLs which recognized only

214 Mn(II), Mn(III), and Mn(IV)) and producing a nonglycosylated variant for detailed biochemical and spe

215 The nonglycosylated variant reached the cell surface by bypa

216 enovirus vector expressing a nonsecreted and nonglycosylated version of MDA-7/IL-24 protein was gener

217 We found that nonglycosylated viruses were attenuated and produced low

218 In contrast, nonglycosylated viruses were attenuated, producing lower

219 urthermore, the glycosylation content (i.e., nonglycosylated vs glycosylated, sialylated vs desialyla

220 from saliva of various races and genders is nonglycosylated with the same molecular weight of 55,881

221 257Y, presumed to be toxic to the cells, and nonglycosylated WT opsin obtained by growth in the prese

222 ith tunicamycin, consistent with the size of nonglycosylated Xenopus laevis alphaENaC.

223 tivity assay conditions, we demonstrate that nonglycosylated XXT2 lacking its cytosolic amino-termina

224 glycosylated COS7-expressed receptor and the nonglycosylated yeast-produced receptor.

225 cytes could contribute to the attenuation of nonglycosylated ZIKV in mice.IMPORTANCE It is unclear wh

226 We generated nonglycosylated ZIKV mutants from contemporary and histo