ライフサイエンスコーパス: nonribosomal peptide synthetase

1 pose a production pathway that is based on a nonribosomal peptide synthetase.

2 ule of the three-subunit (TycABC), 10-module nonribosomal peptide synthetase.

3 d thioester intermediates on a multimodular, nonribosomal peptide synthetase.

4 three-subunit (CepA, CepB, and CepC) modular nonribosomal peptide synthetase.

5 cterization of the termination module of the nonribosomal peptide synthetase.

6 unds are synthesized on ribosomes and not by nonribosomal peptide synthetases.

7 es that are synthesized wholly or in part by nonribosomal peptide synthetases.

8 ra- and interchain activities within dimeric nonribosomal peptide synthetases.

9 to one of two genes, orbI and orbJ, encoding nonribosomal peptide synthetases.

10 cyl-CoA synthetases, firefly luciferase, and nonribosomal peptide synthetases.

11 bleomycin are assembled by hybrid polyketide/nonribosomal peptide synthetases.

12 , and the adenylation domains of the modular nonribosomal peptide synthetases.

13 zation states between monomers and dimers in nonribosomal peptide synthetases.

14 domains has been less well characterized in nonribosomal peptide synthetases.

15 to the adenylation and thiolation domains of nonribosomal peptide synthetases.

16 sis, functionally similar to the present day nonribosomal peptide synthetases.

17 ion and condensation domain found in certain nonribosomal peptide synthetases.

18 odular enzymes like polyketide synthases and nonribosomal peptide synthetases.

19 y-guided approach has been unprecedented for nonribosomal peptide synthetases.

20 tide synthases, in contrast to the monomeric nonribosomal peptide synthetases.

21 ed to predict the substrate specificities of nonribosomal peptide synthetase adenylation (A) domains

22 s approach, we screened syn-BNPs inspired by nonribosomal peptide synthetases against microbial patho

23 chimeric multimodular polyketide synthase, a nonribosomal peptide synthetase, along with enzymes resp

24 e of a C(modAA) domain, which is part of the nonribosomal peptide synthetase AmbE in the biosynthesis

25 A, DszB, and DszC, with DszC containing both nonribosomal peptide synthetase and polyketide synthase

26 al system for exploring interactions between nonribosomal peptide synthetase and polyketide synthase

27 The hybrid nonribosomal peptide synthetase and polyketide synthase

28 These multidomain enzymes, known as nonribosomal peptide synthetases and polyketide synthase

29 diverse types of natural products, including nonribosomal peptide synthetases and polyketide synthase

30 our understanding of the structural cycle of nonribosomal peptide synthetases and provide insights in

31 uperfamily that includes firefly luciferase, nonribosomal peptide synthetases, and acyl- and aryl-CoA

32 s of every known formally classified family, nonribosomal peptide synthetases, and several hybrid clu

33 tension of this methodology to other dimeric nonribosomal peptide synthetases, and to the related fat

34 er, with ppzA, encoding a peramine-producing nonribosomal peptide synthetase, as the central componen

35 Thus, full reconstitution of the nonribosomal peptide synthetase assembly line by purifie

36 editing pathway for this polyketide synthase/nonribosomal peptide synthetase assembly line.

37 and synthesis of cyclic peptides inspired by nonribosomal peptide synthetase BGCs associated with the

38 Nonribosomal peptide synthetase bioengineering could lea

39 analyses of algal cultures, we identified a nonribosomal peptide synthetase biosynthetic gene cluste

40 emodeling, we successfully expressed a 67-kb nonribosomal peptide synthetase biosynthetic gene cluste

41 ally derived modular polyketide synthase and nonribosomal peptide synthetase biosynthetic pathways ar

42 none substrates originating from a bimodular nonribosomal peptide synthetase (BraB or PxaA).

43 ificity of gatekeeper adenylation domains of nonribosomal peptide synthetases can be switched from an

44 metal center not previously associated with nonribosomal peptide synthetase chemistry.

45 We show that nonribosomal peptide synthetases ClbN and ClbB assemble

46 the genes for biosynthesis of L-PAPA and the nonribosomal peptide synthetase CmlP.

47 brio cholerae is assembled by a four-subunit nonribosomal peptide synthetase complex, VibE, VibB, Vib

48 The nonribosomal peptide synthetase consisted of a priming m

49 onal changes in thioesterase domains of such nonribosomal peptide synthetases control the oligomeriza

50 ncation of the carboxy terminus carrying the nonribosomal peptide synthetase cores, as well as the si

51 of 16 ORFs, encoding proteins homologous to nonribosomal peptide synthetases, cytochrome P450-relate

52 The pathway builds on a nonribosomal peptide synthetase de-rived di-tyrosine pip

53 nting the primarily polyketide synthase- and nonribosomal peptide synthetase-derived universe of micr

54 The largest continuous bacterial nonribosomal peptide synthetase discovered so far is des

55 s of the method are demonstrated on a 37 kDa nonribosomal peptide synthetase domain subject to spectr

56 0 containing genes encoding for a six-module nonribosomal peptide synthetase EcdA, an acyl-AMP ligase

57 ed a lipopeptide of 1,928 Da to an 18-module nonribosomal peptide synthetase encoded as a single gene

58 ydroxybenzoate (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF.

59 the phenylalanine adenylation domain of the nonribosomal peptide synthetase enzyme gramicidin S synt

60 s a natural modification of the product of a nonribosomal peptide synthetase enzyme, whose consequenc

61 ation of such heterocyclization ability in a nonribosomal peptide synthetase enzyme.

62 important subset of polyketide synthases and nonribosomal peptide synthetases evolve by concerted evo

63 osynthesis, including the modular polyketide-nonribosomal peptide synthetase for necroxime assembly.

64 VbsS, a nonribosomal peptide synthetase free-standing module, th

65 n of free piperazic acid with a three-domain nonribosomal peptide synthetase from the incarnatapeptin

66 0 genome identified a 59-kb cryptic inactive nonribosomal peptide synthetase gene cluster proposed to

67 amycin, a predicted lipopeptide encoded by a nonribosomal peptide synthetase gene cluster.

68 sly, we found a widely distributed family of nonribosomal peptide synthetase gene clusters in gut bac

69 , 157 syn-BNP cyclic peptides inspired by 96 nonribosomal peptide synthetase gene clusters were synth

70 ified a cluster of nine genes (including one nonribosomal peptide synthetase gene, ataP) that is requ

71 compounds of this type are biosynthesized by nonribosomal peptide synthetases, giant enzyme complexes

72 We deleted gliP, the gene that encodes the nonribosomal peptide synthetase GliP.

73 ies during assembly by polyketide synthases, nonribosomal peptide synthetases, glycosyltransferases a

74 Nonribosomal peptide synthetase heterocyclization (Cy) d

75 s high molecular weight protein 2 (HMWP2), a nonribosomal peptide synthetase homologue, and YbtE in t

76 tic pathways for both siderophores involve a nonribosomal peptide synthetase independent siderophore

77 biosynthesized using the recently described nonribosomal peptide synthetase independent siderophore

78 this unique acyl carrier protein-dependent, nonribosomal peptide synthetase-independent biosynthetic

79 Here we uncover a nonribosomal peptide synthetase-independent biosynthetic

80 synthesizes rhizoferrin and encodes a single nonribosomal peptide synthetase-independent siderophore

81 fslA, encodes a member of the superfamily of nonribosomal peptide synthetase-independent siderophore

82 , we disclose a three-dimensional model of a nonribosomal peptide synthetase-independent siderophore

83 in the synthesis of aminoacyl-thioesters by nonribosomal peptide synthetases is relaxed, whereas the

84 NPS6, encoding a nonribosomal peptide synthetase, is a virulence determin

85 for engineering hybrid PKS-PKS and PKS-NRPS (nonribosomal peptide synthetase) junctions and suggests

86 ubstrates that load carrier domains found in nonribosomal peptide synthetases, large molecule mass sp

87 Subsequent enzymatic assays with the nonribosomal peptide synthetase-like AsbC, AsbD, and Asb

88 Here, we discovered an unprecedented nonribosomal peptide synthetase-like-pteridine synthase

89 ial DNA gyrase is assembled from tyrosine by nonribosomal peptide synthetase logic.

90 the AMP-forming family of enzymes, including nonribosomal peptide synthetases, luciferase, and aryl-

91 iron scavenger (siderophore) and product of nonribosomal peptide synthetase machinery, to the C-term

92 ace between its loading module, which uses a nonribosomal peptide synthetase mechanism, and its initi

93 e synthase module (KS-AT-MT(2)-KR-ACP) and a nonribosomal peptide synthetase module (Cy(3)-MT(3)-PCP(

94 inary biochemical analysis of the N-terminal nonribosomal peptide synthetase module from the Thalasso

95 Here we show that DdaD, a nonribosomal peptide synthetase module, activates and se

96 l proteins composed of a loading module, one nonribosomal peptide synthetase module, eight polyketide

97 slationally modifies polyketide synthase and nonribosomal peptide synthetase modules.

98 ll be used in mechanistic studies of the two nonribosomal peptide synthetases NocA and NocB that lie

99 microbiome analysis, we identified a hybrid nonribosomal peptide synthetase (NRPS) and polyketide sy

100 They are synthesized on modular nonribosomal peptide synthetase (NRPS) and polyketide sy

101 ilis on an enzymatic assembly line with both nonribosomal peptide synthetase (NRPS) and polyketide sy

102 g a PCR approach to clone a thiazole-forming nonribosomal peptide synthetase (NRPS) as a probe, we lo

103 ielding a pentapeptide on the thio-templated nonribosomal peptide synthetase (NRPS) assembly line pro

104 ptide aglycone or intermediates bound to the nonribosomal peptide synthetase (NRPS) assembly line.

105 re any of the canonical epimerase domains of nonribosomal peptide synthetase (NRPS) assembly lines fo

106 orin A heterodimer are made via two distinct nonribosomal peptide synthetase (NRPS) assembly lines, a

107 FUM14 was predicted to encode a nonribosomal peptide synthetase (NRPS) containing two do

108 the highly dissociated nature of the encoded nonribosomal peptide synthetase (NRPS) domains and modul

109 ly identified fungal anthranilate-activating nonribosomal peptide synthetase (NRPS) domains through b

110 olyketide, a reaction typically catalyzed by nonribosomal peptide synthetase (NRPS) domains.

111 All three are homologous to nonribosomal peptide synthetase (NRPS) domains: VibE is

112 biosynthesized by polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) enzymes EpoA-F.

113 The activity of several nonribosomal peptide synthetase (NRPS) enzymes is used t

114 ituting amino acid-specifying domains within nonribosomal peptide synthetase (NRPS) enzymes.

115 s (CDA) are cyclic lipopeptides assembled by nonribosomal peptide synthetase (NRPS) enzymes.

116 Ebony is a rare example of a nonribosomal peptide synthetase (NRPS) from a higher euk

117 genome of Vibrio harveyi BAA-1116 contains a nonribosomal peptide synthetase (NRPS) gene cluster (aeb

118 obiosynthetic analyses identified a putative nonribosomal peptide synthetase (NRPS) gene cluster (nan

119 AS), as the major product of the cryptic has nonribosomal peptide synthetase (NRPS) gene cluster in t

120 ions about the polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) genes in which th

121 ucts produced by a polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) hybrid involving

122 Polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) hybrid systems ty

123 Genetic engineering of the nonribosomal peptide synthetase (NRPS) in the daptomycin

124 ecture and domain organization of the ncpA-B nonribosomal peptide synthetase (NRPS) is co-linear in a

125 d high expression of a horizontally acquired nonribosomal peptide synthetase (NRPS) locus.

126 f yersiniabactin (Ybt) synthetase, a 230 kDa nonribosomal peptide synthetase (NRPS) making the N-term

127 by Vibrio anguillarum, is synthesized via a nonribosomal peptide synthetase (NRPS) mechanism.

128 h a predicted acyltransferase, a stand-alone nonribosomal peptide synthetase (NRPS) module, and four

129 first transient-state kinetic analysis of a nonribosomal peptide synthetase (NRPS) module.

130 teins (PacA-V), including highly dissociated nonribosomal peptide synthetase (NRPS) modules and a var

131 e 56-kb gene cluster encodes a series of six nonribosomal peptide synthetase (NRPS) modules distribut

132 (IbetaH(Asp)) at the carboxyl terminus of a nonribosomal peptide synthetase (NRPS) or as stand-alone

133 ynthesized via polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) pathways.

134 that the reductive domain from a monomodular nonribosomal peptide synthetase (NRPS) protein FmtATR pe

135 Many bacteria use nonribosomal peptide synthetase (NRPS) proteins to produ

136 the 23 358 bp cymA, which encodes a 7-module nonribosomal peptide synthetase (NRPS) responsible for a

137 enerated by hybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) systems in strept

138 ys where the PKS is fused to a single module nonribosomal peptide synthetase (NRPS) to synthesize pol

139 The six-domain, 270 kDa nonribosomal peptide synthetase (NRPS) VibF, a component

140 (TE) domain from the multidomain tyrocidine nonribosomal peptide synthetase (NRPS) was recently show

141 -3, is the nascent product of the LNM hybrid nonribosomal peptide synthetase (NRPS)-acyltransferase (

142 arylamine precursor of chloramphenicol in a nonribosomal peptide synthetase (NRPS)-based pathway to

143 dentified hundreds of such thiotemplated yet nonribosomal peptide synthetase (NRPS)-independent biosy

144 cing polyketide synthase (HRPKS, Fub1) and a nonribosomal peptide synthetase (NRPS)-like carboxylic a

145 an SH domain (LnmJ-SH) within the LNM hybrid nonribosomal peptide synthetase (NRPS)-polyketide syntha

146 d by a three-protein (EntE, B, F) six-module nonribosomal peptide synthetase (NRPS).

147 planarians revealed niche cells expressing a nonribosomal peptide synthetase (nrps).

148 F, which originates from a fungal trimodular nonribosomal peptide synthetase (NRPS).

149 ves assembly of the MBT backbone by a hybrid nonribosomal peptide synthetase (NRPS)/polyketide syntha

150 ering biosynthetic assembly lines, including nonribosomal peptide synthetases (NRPS) and related mega

151 Type II nonribosomal peptide synthetases (NRPS) generate exotic

152 In the quest for new bioactive substances, nonribosomal peptide synthetases (NRPS) provide biodiver

153 inone-fused ring systems can be assembled by nonribosomal peptide synthetases (NRPS) using the confor

154 Nonribosomal peptide synthetases (NRPS), fatty acid synt

155 Nonribosomal peptide synthetases (NRPS), fatty acid synt

156 Nonribosomal peptide synthetases (NRPS), polyketide synt

157 erstanding of domain-domain communication in nonribosomal peptide synthetases (NRPSs) and lay the gro

158 Nonribosomal peptide synthetases (NRPSs) and NRPS-like e

159 Single-module nonribosomal peptide synthetases (NRPSs) and NRPS-like e

160 Nonribosomal peptide synthetases (NRPSs) and polyketide

161 tic gene clusters encoding the production of nonribosomal peptide synthetases (NRPSs) and polyketide

162 Nonribosomal peptide synthetases (NRPSs) and polyketide

163 and antifungal properties are synthesized by nonribosomal peptide synthetases (NRPSs) and polyketide

164 Nonribosomal peptide synthetases (NRPSs) are a family of

165 Nonribosomal peptide synthetases (NRPSs) are large, mult

166 Nonribosomal peptide synthetases (NRPSs) are microbial e

167 Nonribosomal peptide synthetases (NRPSs) are modular ass

168 Nonribosomal peptide synthetases (NRPSs) are multimodula

169 The nonribosomal peptide synthetases (NRPSs) are one of the

170 Nonribosomal peptide synthetases (NRPSs) are responsible

171 Polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) are two related

172 Nonribosomal peptide synthetases (NRPSs) assemble a larg

173 Ribosomes and nonribosomal peptide synthetases (NRPSs) carry out instr

174 Nonribosomal peptide synthetases (NRPSs) catalyze the bi

175 he C-terminal thioesterase (TE) domains from nonribosomal peptide synthetases (NRPSs) catalyze the fi

176 Modular polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) comprise giant

177 Nonribosomal peptide synthetases (NRPSs) generate the co

178 of linear dipeptidyl precursors derived from nonribosomal peptide synthetases (NRPSs) into 2,5-diketo

179 Nonribosomal peptide synthetases (NRPSs) make many natur

180 intermediates on the carrier domains of the nonribosomal peptide synthetases (NRPSs) or polyketide s

181 Nonribosomal peptide synthetases (NRPSs) produce a wide

182 In mycobacteria, polyketide synthases and nonribosomal peptide synthetases (NRPSs) produce complex

183 lly diverse acidic lipopeptides assembled by nonribosomal peptide synthetases (NRPSs) that are known

184 In comparison with the large number of nonribosomal peptide synthetases (NRPSs) that release th

185 Nonribosomal peptide synthetases (NRPSs) underlie the bi

186 Nonribosomal peptide synthetases (NRPSs) use phosphopant

187 ymes, including polyketide synthases (PKSs), nonribosomal peptide synthetases (NRPSs), and mixed PKS/

188 ynthetic gene cluster predicted that the two nonribosomal peptide synthetases (NRPSs), BlmIV and BlmI

189 terrogation of enzyme-bound intermediates in nonribosomal peptide synthetases (NRPSs), mass spectrome

190 ists of 34 genes encoding fatty acid ligase, nonribosomal peptide synthetases (NRPSs), regulators, tr

191 ty of the multidomain enzyme assembly lines, nonribosomal peptide synthetases (NRPSs), to utilize bes

192 biosynthesized from amino acid precursors by nonribosomal peptide synthetases (NRPSs), which are orga

193 lies to multidomain enzymatic systems called nonribosomal peptide synthetases (NRPSs), which assemble

194 by large multi-enzyme assembly lines called nonribosomal peptide synthetases (NRPSs).

195 onstandard amino acids that are assembled by nonribosomal peptide synthetases (NRPSs).

196 bitors that like muraymycin are derived from nonribosomal peptide synthetases (NRPSs).

197 t involves large, multidomain enzymes called nonribosomal peptide synthetases (NRPSs).

198 Here we report the structure of the nonribosomal peptide synthetase ObiF1, highlighting the

199 irs of Esx secreted proteins, a multisubunit nonribosomal peptide synthetase operon, and genes encodi

200 k marks the first direct characterization of nonribosomal peptide synthetase oxidase domain activity

201 Paenibacterin is biosynthesized by a nonribosomal peptide synthetase pathway.

202 the possibility of future engineering within nonribosomal peptide synthetase pathways using oxidase d

203 paS, a hybrid two module polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) that generate

204 is a hybrid, two module polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) that makes an

205 ins only a single-module polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS), although two

206 ructure encodes a hybrid polyketide synthase nonribosomal peptide synthetase (PKS-NRPS), which resemb

207 eosome inhibitors biosynthesized by a hybrid nonribosomal peptide synthetase-polyketide synthase (NRP

208 f Pseudovibrio genomes contain a member of a nonribosomal peptide synthetase-polyketide synthase gene

209 he AEST biosynthetic gene cluster encoding a nonribosomal peptide synthetase/polyketide synthase acco

210 ey are synthesized by the action of a hybrid nonribosomal peptide synthetase/polyketide synthase in t

211 A) followed by backbone assembly by a hybrid nonribosomal peptide synthetase/polyketide synthase.

212 s-specific biosynthetic loci encoding hybrid nonribosomal peptide synthetase/polyketide synthases con

213 RifR, a type II thioesterase from the hybrid nonribosomal peptide synthetases/polyketide synthase rif

214 st highly induced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.

215 Nonribosomal peptide synthetases produce diverse natural

216 The C-terminal thioesterase domain of the nonribosomal peptide synthetase producing the lipopetide

217 tested on a 500 microM sample of the 33 kDa nonribosomal peptide synthetase protein EntB.

218 Multiple motifs characteristic of the nonribosomal peptide synthetase protein family were iden

219 The excised TE domain from the nonribosomal peptide synthetase responsible for the prod

220 CP2 thiolation domains of PchE and PchF, the nonribosomal peptide synthetases responsible for the bio

221 Nonribosomal peptide synthetases responsible for the pro

222 ion studies revealed the central role of the nonribosomal peptide synthetase Sln9 in constructing and

223 des harboring covalently modified serines in nonribosomal peptide synthetases, streamlined methodolog

224 All six domains of this nonribosomal peptide synthetase subunit, three phosphopa

225 e is assembled by a four-subunit, ten-domain nonribosomal peptide synthetase system, VibE, VibB, VibH

226 residue is installed into the scaffold by a nonribosomal peptide synthetase that also performs the s

227 denylation (A) domain of the Yersinia pestis nonribosomal peptide synthetase that biosynthesizes the

228 Here, we uncover a nonribosomal peptide synthetase that is induced in male

229 ts that are often produced by enzymes called nonribosomal peptide synthetases that many pathogenic ba

230 clusters resembling bacterial polyketide and nonribosomal peptide synthetases that produce antibiotic

231 esterase domains of polyketide synthases and nonribosomal peptide synthetases to macrocycles via cova

232 synthases and one hybrid polyketide synthase-nonribosomal peptide synthetase together with cytochrome

233 y SgcC1 homologous to adenylation domains of nonribosomal peptide synthetases, was identified as spec

234 s the approximately 450 residue E domains of nonribosomal peptide synthetases were constructed, and t

235 Two biosynthesis gene clusters encoding nonribosomal peptide synthetases were identified, emphas

236 gopeptin and syringomycin are synthesized by nonribosomal peptide synthetases which are encoded by th

237 ntains an unusual hybrid polyketide synthase-nonribosomal peptide synthetase, which resembles iterati

238 We identify a nonribosomal peptide synthetase with an unusual domain a