ライフサイエンスコーパス: nucleocapsid

1 gain access to the genome sequestered in the nucleocapsid.

2 n and other viral proteins to form a helical nucleocapsid.

3 and appears to form a core in the center of nucleocapsid.

4 in conserved regions of membrane, spike, and nucleocapsid.

5 ts bullet shaped particle contains a helical nucleocapsid.

6 cription solely upon local disruption of the nucleocapsid.

7 binding-register of viral genomic RNA within nucleocapsids.

8 ired for efficient nuclear egress of progeny nucleocapsids.

9 on reconstructions of complete mononegavirus nucleocapsids.

10 he nucleoprotein (N), giving rise to helical nucleocapsids.

11 in the nuclear release or transport of viral nucleocapsids.

12 large RNA polymerase (L) decorate the viral nucleocapsids.

13 sary for efficient nuclear egress of progeny nucleocapsids.

14 and packaging of the viral genome into viral nucleocapsids.

15 d that these gaps frequently contained viral nucleocapsids.

16 latory mechanisms of nuclear egress of viral nucleocapsids.

17 the circularized DNAs that are packaged into nucleocapsids.

18 s largely exposed in fully assembled measles nucleocapsids.

19 We show that nucleocapsid AC141 associates with the lepidopteran Tric

20 The nucleocapsid acts as a scaffold for virus assembly and a

21 nct HLA-A*02:01/HBV epitope complexes of HBV nucleocapsid and envelope proteins, we mapped their topo

22 ata to generate models of HIV-1 genomic RNA, nucleocapsid and integrase condensed into a mature ribon

23 Finally, nucleic acids interact with both nucleocapsid and matrix domains, and proteolytic process

24 es from 6 patients with COVID-19 showed anti-nucleocapsid and spike protein antibodies appearing betw

25 rements of plasma or serum antibodies to the nucleocapsid and spike proteins were analyzed using luci

26 highly complex structural layer between the nucleocapsid and the envelope of virions.

27 ment, a complex structural layer between the nucleocapsid and the envelope within virus particles.

28 g hemifusion but failing to uncoat the viral nucleocapsid and to replicate in host nuclei.

29 the site of virion assembly and coordinates nucleocapsid and virus formation.

30 These viral inclusions contain the EBOV nucleocapsids and are sites of viral replication and nuc

31 nfected nuclei results in coiling of genomic nucleocapsids and suppression of viral transcription.

32 is necessary for pgRNA packaging into viral nucleocapsids and the initiation of viral reverse transc

33 , packaging of viral RNA with C protein into nucleocapsid, and budding of prM and E proteins into vir

34 spike receptor binding domain (RBD), S1+S2, nucleocapsid, and ORF6 to ORF10 of SARS-CoV-2, to the HC

35 ore analyzed cellular responses to membrane, nucleocapsid, and spike proteins in individuals sufferin

36 zee-adenovirus-155, encoding RSV fusion (F), nucleocapsid, and transcription antitermination proteins

37 r with other antibodies such as RBD, S1, and nucleocapsid antibody nor with proteins such as interleu

38 Specificity was higher using the nucleocapsid antibody tests (99.3% and 99.7%) than using

39 Two nucleocapsid antibody tests (Abbott IgG and Roche total

40 In conclusion, two nucleocapsid antibody tests outperformed a spike protein

41 The two nucleocapsid antibody tests were more sensitive than the

42 tor System for Rapid Detection of SARS-CoV-2 nucleocapsid antigen (Veritor), a chromatographic immuno

43 A recombinant nucleocapsid antigen specifically captures SARS-CoV-2 an

44 ) vectors co-expressing SARS-CoV-2 spike and nucleocapsid antigens, two immunodominant antigens impli

45 The stability of the genomic RNA in the nucleocapsid appears to regulate its accessibility to th

46 on mediated by the viral glycoproteins, HMPV nucleocapsids are released into the cell cytoplasm.

47 Nucleocapsids are too large to diffuse in the cytoplasm

48 nica multiple nucleopolyhedrovirus (AcMNPV), nucleocapsids are transported through the cell.

49 esults indicate that the mechanisms by which nucleocapsids are transported to the farthest reaches of

50 ing spacer peptide 1 that connects capsid to nucleocapsid, are intrinsically disordered.

51 l RdRp uses the genomic RNA inside the viral nucleocapsid as the template to synthesize viral RNAs.

52 LK1 is associated with the biogenesis of the nucleocapsid, as BI-2536 leads to its decreased intracel

53 on between VP24 and NP was required for both nucleocapsid assembly and genome packaging.

54 s inhibitors showed intact nuclear stages of nucleocapsid assembly but the cytoplasmic virus maturati

55 ggested that both SBAs and BAs inhibited HBV nucleocapsid assembly by binding to the heteroaryldihydr

56 core protein that play roles in defining the nucleocapsid assembly pathway.

57 rms a structured oligomer that is suited for nucleocapsid assembly, and phosphorylated protein forms

58 ng the organization of the pre-genome during nucleocapsid assembly, facilitating subsequent reverse t

59 es in particular, the mechanistic process of nucleocapsid assembly, RNA encapsidation, and the roles

60 ption and acting as a nucleation complex for nucleocapsid assembly.

61 d protein, SD1, plays a critical role in the nucleocapsid assembly.

62 ion was associated with enhanced exposure of nucleocapsid-associated DNA, the exposed viral DNA indee

63 AC141 is a nucleocapsid-associated protein required for BV egress a

64 in-dependent long-distance transport of EBOV nucleocapsids before budding at the cell surface.

65 e involved in membrane targeting, packaging, nucleocapsid binding, and proton transport.

66 ages of these mutants showed assembled viral nucleocapsids but no completed, mature virions.

67 izes the 5'-triphosphorylated dsRNA on FLUAV nucleocapsids but that polymorphisms at position 627 of

68 it positions the polymerase complex onto the nucleocapsid, but also acts as a chaperone for the nucle

69 act HIV-1 cores or in vitro-assembled capsid-nucleocapsid (CA-NC) complexes.

70 ivity to SUN1, and in vitro-assembled capsid-nucleocapsid (CANC) nanotubes captured SUN1 and SUN2 fro

71 ading frame (dORF) located downstream of the nucleocapsid coding region.

72 r, DRFs also bound in vitro assembled capsid-nucleocapsid complexes and promoted the disassembly of H

73 act HIV-1 cores or in vitro assembled capsid-nucleocapsid complexes, while EB1 did not.

74 Furthermore, coexpression of the nucleocapsid component VP35 overcomes deletion of NP-Ct

75 s reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation

76 in-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and access

77 The nucleocapsid conformation depends on the reverse transcr

78 es have a highly organized structure, with a nucleocapsid core containing the RNA genome surrounded b

79 highly specific and suggest that cognate MP-nucleocapsid core protein interactions are required for

80 hese interactions are able to redirect viral nucleocapsid core proteins from their sites of replicati

81 d that they interact only with their cognate nucleocapsid core proteins.

82 ns of binding sites in cytoplasmic vs. viral nucleocapsids demonstrate that budding causes discrete c

83 avian FLUAV restriction factor and highlight nucleocapsid disruption as an antiviral strategy.

84 cccDNA synthesis from intracellular progeny nucleocapsid DNA.

85 g domain; and the C-terminal, genome-binding nucleocapsid domain.

86 ing the matrix, capsid, spacer peptide 1 and nucleocapsid domains (referred to as DeltaGag) by hetero

87 sport systems may be involved in baculovirus nucleocapsid egress and BV formation.

88 Marginally located nucleocapsids entered filopodia, moved inside, and budde

89 a bacilliform virus containing a rod shaped nucleocapsid enveloped in an elliptical membrane.

90 C protein assembles onto genomic RNA to form nucleocapsid, followed by prM and E envelopment and viri

91 RNA (pgRNA) and DNA polymerase complex into nucleocapsids for reverse transcriptional DNA replicatio

92 lusion bodies (IBs) are cytoplasmic sites of nucleocapsid formation and RNA replication, housing key

93 omains (CTD) of capsid proteins regulate the nucleocapsid formation.

94 and is membrane enveloped, while SSRV1 has a nucleocapsid formed by a homodimer and is not enveloped.

95 he size of the RNP globule, and exclusion of nucleocapsid from regions with RNA secondary structure d

96 gress complex (NEC) for efficient transit of nucleocapsids from the nucleus to the cytoplasm.

97 Viral titer estimation, nucleocapsid gene amplification, and viral antinucleocap

98 s packaged and protected by long filamentous nucleocapsid-helix structures (RNPs).

99 ht into the architecture of the rabies virus nucleocapsid highlights the surprising structural diverg

100 The presence of anti-spike or anti-nucleocapsid IgG antibodies was associated with a substa

101 Rate ratios were similar when the anti-nucleocapsid IgG assay was used alone or in combination

102 ed by anti-spike (primary analysis) and anti-nucleocapsid IgG assays, and staff members were followed

103 earman rho = 0.386; P < .001) than with anti-nucleocapsid IgG avidity (Spearman rho = 0.211; P = .026

104 SARS-CoV-2 anti-spike and anti-nucleocapsid IgG titers and avidity were measured in a l

105 Gag domains outside the CA (e.g., matrix and nucleocapsid) impact Gag oligomerization as well as imma

106 st the S-protein, its RBD-subunit, and viral nucleocapsid in a cohort of COVID-19 convalescent patien

107 r CLPs in bacteria but failed to form stable nucleocapsids in hepatoma cells.

108 ribution of vesicular stomatitis virus (VSV) nucleocapsids in the cytoplasm of infected cells was ana

109 in filaments are responsible for mobility of nucleocapsids in the cytoplasm, but that actin filaments

110 ier for most DNA viruses that assemble their nucleocapsids in the nucleus.

111 ncy entry of BV and the retention of progeny nucleocapsids in the perinuclear space during egress.

112 rogeny, which results in the accumulation of nucleocapsids in the T cell nucleus.

113 ered, resulting in the accumulation of viral nucleocapsids in the T cell nucleus.

114 was readily detectable in the densely packed nucleocapsids inside perinuclear viral inclusions and in

115 In this study, we examined whether nucleocapsids interact with lepidopteran kinesin-1 motor

116 status of the genome, which in turn controls nucleocapsid interaction with the envelope proteins for

117 to membrane fusion and delivery of the viral nucleocapsid into the cellular cytoplasm.

118 ution in the cytoplasm, the incorporation of nucleocapsids into virions as determined in pulse-chase

119 r role than microtubules in incorporation of nucleocapsids into virions.

120 Prior studies have shown that the entry of nucleocapsids involves the polymerization of actin to pr

121 Formation of the hepatitis B virus nucleocapsid is an essential step in the viral lifecycle

122 The SIFV nucleocapsid is formed by a heterodimer of two homologou

123 sttranslational processing of the spikes and nucleocapsid is necessary to produce infectious virus.

124 a common mechanism of how the growth of the nucleocapsid is orchestrated, and highlight an interacti

125 nd E3 glycoproteins before assembly with the nucleocapsid is the key to producing fusion-competent ma

126 oteolytic processing at the spacer peptide 1|nucleocapsid junction by HIV-1 protease is accelerated i

127 Nucleocapsids labeled with VP39 fused with three copies

128 capsid within intact viruses and recombinant nucleocapsid-like assemblies.

129 c acid, is thereby shown to be essential for nucleocapsid-like particle assembly.

130 The structure of an empty mumps virus nucleocapsid-like particle is determined to 10.4 A resol

131 Previous studies of NCs or nucleocapsid-like particles (NCLPs) from RSV and other n

132 developed approach to assemble measles virus nucleocapsid-like particles on specific sequences of RNA

133 After aggregation to form nucleocapsid-like particles upon incubation with an olig

134 cryo-electron microscopy of nucleocapsid or nucleocapsid-like structures.

135 In addition to NP, other components of the nucleocapsid localize to IBs, including VP35, VP24, VP30

136 ral inclusions and in the dispersed rod-like nucleocapsids located outside of viral inclusions.

137 psids and are sites of viral replication and nucleocapsid maturation.

138 in vitro trackable assembly of RSV-specific nucleocapsids may enable in-depth mechanistic analyses o

139 The role of actin filaments in nucleocapsid mobility was also confirmed by live-cell im

140 here that, somewhat surprisingly, the viral nucleocapsid (N) and phosphoprotein (P) genes together c

141 CoV antigens, mostly derived from SARS-CoV-2 nucleocapsid (N) and spike (S).

142 utralizing titers and antibodies against the nucleocapsid (N) and the receptor binding domain (RBD) o

143 Here, we report a unique phenomenon of PEDV nucleocapsid (N) cleavage by the PEDV-encoded 3C-like pr

144 IgG, IgM and IgA antibodies, as well as anti-nucleocapsid (N) IgG antibody, while children with and w

145 onstrate that the 3'-terminal portion of the nucleocapsid (N) mRNA of Rift Valley fever virus, a phle

146 for NF-kappaB regulation and determined the nucleocapsid (N) protein as the NF-kappaB activator.

147 lation of plasmid DNA vaccine expressing IBV nucleocapsid (N) protein by the QAC adjuvant system (pQA

148 assay was conceived for Spike (S) protein or Nucleocapsid (N) protein detection using magnetic beads

149 d interactions of M with itself and with the nucleocapsid (N) protein drive virus assembly and buddin

150 The SARS-CoV-2 nucleocapsid (N) protein is highly homologous to the N p

151 ctive and respiratory syndrome virus (PRRSV) nucleocapsid (N) protein is the main component of the vi

152 The nucleocapsid (N) protein of coronaviruses serves two maj

153 sor was fabricated by immobilizing the virus nucleocapsid (N) protein on carbon nanofiber-modified sc

154 eading frame that overlaps that of the viral nucleocapsid (N) protein thus limiting options for mutag

155 reover, GFP-MxA condensates included the VSV nucleocapsid (N) protein, a protein previously shown to

156 matitis virus (VSV)-infected Huh7 cells, the nucleocapsid (N) protein, which participates in forming

157 Our analysis identified the spike (S) and nucleocapsid (N) proteins as promising targets for deopt

158 Since the nucleocapsid (N) proteins of these viruses show the grea

159 e, we highlight the use of VIStEDD for nsp6, nucleocapsid (N), and spike (S) surface glycoprotein.

160 r PEDV structural proteins, i.e., spike (S), nucleocapsid (N), membrane (M), and envelope (E).

161 V-2 S1 subunit of the spike glycoprotein and nucleocapsid (N)-based ELISAs not only showed high speci

162 k of cellular and spliced viral RNAs via its nucleocapsid (NC) domain and drives gRNA encapsidation a

163 Disruption of the viral nucleocapsid (NC) domain integrity affects HIV-1 budding

164 The primary NLS is in the nucleocapsid (NC) domain of Gag and binds directly to im

165 lecules but is facilitated by binding of the nucleocapsid (NC) domain to nucleic acid.

166 teractions of the highly flexible NA-binding nucleocapsid (NC) domain.

167 5'-leader of the unspliced viral RNA and the nucleocapsid (NC) domains of a small number of assemblin

168 ediated predominantly by the capsid (CA) and nucleocapsid (NC) domains, which have conserved structur

169 ed domains-the matrix (MA), capsid (CA), and nucleocapsid (NC) domains-drives different phases of vir

170 tion of the N-terminal MA and the C-terminal nucleocapsid (NC) of Gag with the bilayer, since both ar

171 In contrast, HIV-1 nucleocapsid (NC) promotes formation of the extended dim

172 gion (5' UTR) of HIV-1 RNA that are bound by nucleocapsid (NC) protein, which is derived from Gag dur

173 tive proteins: the matrix (MA), capsid (CA), nucleocapsid (NC), and p6 proteins.

174 ional domains: matrix (MA), capsid (CA), and nucleocapsid (NC).

175 of the pregenomic RNA (pgRNA) into immature nucleocapsids (NC), which are converted to mature NCs co

176 facilitate viral RNA packaging into immature nucleocapsids (NCs) and the early stage of viral DNA syn

177 syncytial virus (RSV) polymerase are helical nucleocapsids (NCs), formed by viral RNAs that are encap

178 the host cell and (ii) egress and budding of nucleocapsids newly produced from the plasma membrane.

179 M proteins interact with the nucleocapsid (NP or N) components of vRNPs, and these in

180 f full-length recombinant metastable helical nucleocapsid of Hantaan virus (Hantaviridae family, Buny

181 In addition, the nucleocapsid of the immature virus is more compact than

182 onfirmed by live-cell imaging of fluorescent nucleocapsids of a virus containing P protein fused to e

183 The mechanism by which nucleocapsids of Autographa californica multiple nucleop

184 nt entry of BV and nuclear egress of progeny nucleocapsids of baculoviruses.

185 Nucleocapsids of nonsegmented negative-strand viruses li

186 erized an intriguing phenomenon in which the nucleocapsids of some PEDV strains are proteolytically p

187 Assembly of paramyxoviral nucleocapsids on the RNA genome is an essential step in

188 cleoprotein, and cryo-electron microscopy of nucleocapsid or nucleocapsid-like structures.

189 were derived from the C-terminus of the HIV nucleocapsid p7 protein (NCp7-F2) and finger 3 of the Sp

190 The resulting synthetic nucleocapsids package one full-length RNA genome for eve

191 e in host cells to initiate viral fusion and nucleocapsid penetration into the cytoplasm.

192 therapy to the clinic), membrane, spike, and nucleocapsid peptides elicited interferon-gamma producti

193 cessary transcriptional machinery to the HBV nucleocapsid promoter to modestly enhance viral pregenom

194 Here, we demonstrate that CCHFV nucleocapsid protein (CCHFV-NP) augments mRNA translatio

195 ure of the N-terminal domain of the MERS-CoV nucleocapsid protein (MERS-CoV N-NTD).

196 ts with PD and determined that measles virus nucleocapsid protein (MVNP) was expressed in 70% of thes

197 Hantavirus nucleocapsid protein (N protein) binds to the 5' caps of

198 The multifunctional CCHFV nucleocapsid protein (N protein) plays a crucial role in

199 e-sense genomic RNA completely coated by the nucleocapsid protein (N) and associated by a phosphoprot

200 possibility that the multivalent RNA-binding nucleocapsid protein (N) from severe acute respiratory s

201 e interpret to reflect the lack of the viral nucleocapsid protein (N) on the template.

202 rovirus genome are encapsidated by the viral nucleocapsid protein (N) to form ribonucleoprotein (RNP)

203 Interaction of viral nucleocapsid protein (N) with this conserved sequence fa

204 c RNA is sequestered within a homopolymer of nucleocapsid protein (N).

205 We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid

206 in (L) and the phosphoprotein (P), while the nucleocapsid protein (NP) encapsulates the viral RNA gen

207 Hantavirus encodes a nucleocapsid protein (NP) to encapsidate the genome and

208 must require a conformational change in the nucleocapsid protein (NP) to make the RNA accessible by

209 Both regions interact with the nucleocapsid protein (NP), an essential component of the

210 benzamides (DISeBAs) as novel HIV retroviral nucleocapsid protein 7 (NCp7) inhibitors.

211 genome (open reading frame 1ab [ORF1ab] and nucleocapsid protein [N]).

212 g assay could be demonstrated before day 12; nucleocapsid protein Abs emerged less consistently.

213 mic RNA segments, each sheathed by the viral nucleocapsid protein and bound by the RNA-dependent RNA-

214 t of the nuclear localization signals in the nucleocapsid protein and distinct inserts in the spike g

215 Tightly packed complexes of nucleocapsid protein and genomic RNA form the core of vi

216 vestigation and characterization of the PEDV nucleocapsid protein and its possible link to cell cultu

217 genes to assess the abilities of Phlebovirus nucleocapsid protein and RNA-dependent RNA polymerase to

218 leavage site exposed on the apex of the HAZV nucleocapsid protein arm domain that is cleaved during H

219 s that a helix structural element in the MuV nucleocapsid protein becomes open when the sequestered R

220 trand, and the interaction is independent of nucleocapsid protein binding.

221 derived from either the PIV5 or Nipah virus nucleocapsid protein C-terminal ends are sufficient to d

222 ies reported here reveal that the hantavirus nucleocapsid protein counteracts the PKR antiviral respo

223 tes in a critical interaction with the viral nucleocapsid protein early in infection.

224 Sera were screened by MERS-CoV nucleocapsid protein enzyme-linked immunosorbent assay a

225 ce that seroreactivity using SARS-CoV-2 anti-nucleocapsid protein IgG and anti-spike IgM assays are g

226 Abs to the receptor-binding domain (RBD) and nucleocapsid protein in addition to conventional isotype

227 Although structures are available for the nucleocapsid protein in complex with RNA, and also for p

228 significant response to SARS-CoV-2 spike or nucleocapsid protein in the ELISPOT assay.

229 Further studies revealed that Andes virus nucleocapsid protein inhibited PKR dimerization, a criti

230 of paramyxovirus particles depends on matrix-nucleocapsid protein interactions which enable efficient

231 tobenzamide thioester that targets the viral nucleocapsid protein NCp7, causing zinc ejection and pre

232 stem-loop structure brought on by the HIV-1 nucleocapsid protein NCp7.

233 Antibody to the nucleocapsid protein of SARS-CoV-2 is more sensitive tha

234 structure, even in the absence of the HIV-1 nucleocapsid protein or other RNA chaperones.

235 interactions between the RNA genome and the nucleocapsid protein regulate the activity of vRdRp, whi

236 symptom onset, antibodies against SARS-CoV-2 nucleocapsid protein showed 100% sensitivity and 100% sp

237 caspase cleavage site, DQVD, within the HAZV nucleocapsid protein that is also conserved in CCHFV.

238 or target for neutralizing Abs) or the viral nucleocapsid protein that is known to be highly immunoge

239 e Abbott Architect immunoassay targeting the nucleocapsid protein were run in 3 SARS-CoV-2 IgG immuno

240 deficient nuclear interactions with the SYNV nucleocapsid protein were unable to suppress transcripti

241 D-19 antigens (spike S1, spike S1S2, and the nucleocapsid protein).

242 ntly, we detected an expansion of SARS-CoV-2 nucleocapsid protein-specific Ab-secreting cells in all

243 on between the coronavirus M protein and the nucleocapsid protein.

244 most epitopes were located in ORF1ab or the nucleocapsid protein.

245 G (IgG) antibodies specific for recombinant nucleocapsid proteins (recN) from hCoVs 229E, NL63, OC43

246 ing cells specific for the hemagglutinin and nucleocapsid proteins appeared in circulation in multipl

247 Emerging models indicate that nucleocapsid proteins of other viruses can form biomolec

248 AC141 or VP39, suggesting that either other nucleocapsid proteins or adaptor proteins may be require

249 ns near the C-terminal ends of paramyxovirus nucleocapsid proteins that are important for matrix prot

250 In addition, the nucleocapsid proteins VP39, FP25, and BV/ODV-C42 were al

251 ond to peptides from the membrane, spike, or nucleocapsid proteins were more common in subjects who d

252 T cell responses to SARS-CoV-2 spike and nucleocapsid proteins were present in only 1 participant

253 rocess that starts with the formation of the nucleocapsid providing a confined space where the viral

254 gamma134.5 gene product of HSV-1 facilitates nucleocapsid release to the cytoplasm through bridging t

255 Consequently, nucleocapsids released by potential superinfectors are s

256 ctions either coils or prevents uncoiling of nucleocapsids released by the superinfecting SYNV virion

257 , and the N- and C-terminal zinc knuckles of nucleocapsid) retain their fold and reorient semi-indepe

258 The structure reveals a 13-mer nucleocapsid ring whose diameter, cavity, and pitch/heig

259 ic, non-infectious virions without affecting nucleocapsid-RNA interactions.

260 nosorbent assay based on the recombinant NiV nucleocapsid (rNiV-N) protein.

261 s in that the genomic RNA sequestered in the nucleocapsid serves as the template.

262 o transcription run-on assay, containing RSV nucleocapsids, showed that AZ-27 inhibits synthesis of t

263 ta analysis indicated the formation of virus-nucleocapsid-sized (or wider) channels extending through

264 ults for the impact of CTD truncation on the nucleocapsid stability.

265 ts of variation were identified in spike and nucleocapsid structural proteins as well as the 3' untra

266 Our thermodynamic analysis of the nucleocapsid structure and stability indicates that appr

267 ase activity without major disruption of the nucleocapsid structure.

268 series of CRISPR-Cas13a-based antibacterial nucleocapsids, termed CapsidCas13a(s), capable of sequen

269 A viruses condense their genome into helical nucleocapsids that constitute essential templates for vi

270 help elucidate the assembly mechanism of the nucleocapsid (the viral RNA genome packaged by the nucle

271 he template RNA is always sequestered in the nucleocapsid, the viral RdRp must find a way to open it

272 viruses likely involves the passage of viral nucleocapsids through MP-gated plasmodesmata, but the mo

273 gy and might help the polymerase remodel the nucleocapsid to allow RNA synthesis to occur efficiently

274 y packages genomic RNA (gRNA) into the viral nucleocapsid to produce infectious virus.

275 We explore the ability of these nucleocapsids to evolve virus-like properties by generat

276 This is likely due to the ability of nucleocapsids to follow shorter paths to the plasma memb

277 volves the polymerization of actin to propel nucleocapsids to nuclear pores and entry into the nucleu

278 lating at the plasma membrane and recruiting nucleocapsids to the budding sites.

279 ubules were responsible for migration of VSV nucleocapsids to the plasma membrane for virus assembly.

280 Although the UL31 and UL34 proteins control nucleocapsid transit in infected cells, the molecular in

281 Until now, no live-cell studies on EBOV nucleocapsid transport have been performed, and particip

282 Nucleocapsid transport was arrested upon depolymerizatio

283 ss, as well as the trajectories and speed of nucleocapsid transport, remain unknown.

284 ication of cellular proteins involved in the nucleocapsid transport.

285 me, viral ribonucleoprotein (RNP) particles (nucleocapsids) travel from their initial sites of synthe

286 ted cells, but the mechanism by which AcMNPV nucleocapsids traverse the cytoplasm is unknown.

287 The intrinsic flexibility of nucleocapsids usually prevents their full-length structu

288 n of green fluorescent protein (GFP)-labeled nucleocapsid viral protein 30 (VP30) in EBOV-infected ce

289 lization studies demonstrated that the Sf-RV nucleocapsid was targeted to plasmodesmata, while two fo

290 Since the core of NP is rigid in the nucleocapsid, we suggest that interactions between this

291 whereas functional antibody responses to the nucleocapsid were elevated in deceased individuals.

292 Nucleocapsids were located near the cell nucleus at earl

293 ells expressing DN NSF revealed that progeny nucleocapsids were retained in a perinuclear space surro

294 EBOV nucleocapsids were visualized by expression of green flu

295 virions, resulting in defective uncoating of nucleocapsid when infecting new cells.

296 s, the genomic RNA is sequestered inside the nucleocapsid when the viral RNA-dependent RNA polymerase

297 The viral nucleocapsid, which is minimally composed of the protein

298 Here we create synthetic nucleocapsids, which are computationally designed icosah

299 inalized by the enclosure of the icosahedral nucleocapsid within a heterogeneous envelope.

300 ng to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucle