ライフサイエンスコーパス: nucleolytic

1 that DNA-PKcs does not protect DNA from the nucleolytic action of WRN.

2 ome integrity tightly controlled by specific nucleolytic activities and central homologous recombinat

3 likely involved in optimized recruitment of nucleolytic activities for the processing and protection

4 a result of this binding, Brca1 inhibits the nucleolytic activities of the Mre11/Rad50/Nbs1 complex,

5 ty and is believed to be responsible for the nucleolytic activities of the RecBCD enzyme.

6 nhibit RecBCD degradation by turning off its nucleolytic activities.

7 sn145 and Glu164, which are crucial for Spd1 nucleolytic activity and shown the active site constella

8 residues in Gre responsible for induction of nucleolytic activity in RNAP.

9 e demonstrate the existence of EndA-mediated nucleolytic activity independent of the competence state

10 indings, we posit a model whereby functional nucleolytic activity is not the feature of Rat1 that ult

11 MUS81 nucleolytic activity is required to activate compensator

12 Cyclophilin nucleolytic activity is stimulated by Ca2+ and/or Mg2+,

13 particles, we have clearly demonstrated that nucleolytic activity is the antiviral mechanism.

14 The nucleolytic activity of animal Argonaute proteins is dee

15 Transcription elongation factor GreA induces nucleolytic activity of bacterial RNA polymerase (RNAP).

16 a member of the RecQ family, stimulates the nucleolytic activity of human exonuclease 1 (hExo1), a 5

17 The nucleolytic activity of MUS81 endonuclease is required f

18 We have now evaluated the nucleolytic activity of recombinant cyclophilins under n

19 nt work, we characterize the RNA-binding and nucleolytic activity of recombinant mouse Ago2.

20 on factors GreA and GreB stimulate intrinsic nucleolytic activity of RNA polymerase (RNAP).

21 The nucleolytic activity of SLX1-SLX4 is negatively regulate

22 ed fusion protein, appeared to have very low nucleolytic activity on single-stranded (ss) DNA, which

23 capsid-associated form of AN which exhibits nucleolytic activity suggests that it may play some role

24 rvations suggest that the exosome may be the nucleolytic activity that degrades the body of the mRNA

25 ly, RPA is also required both to direct Dna2 nucleolytic activity to the 5'-terminated strand of the

26 unit of the MRN complex and orchestrates its nucleolytic activity to the ATR kinase signaling.

27 NTD is responsible for the GreA induction of nucleolytic activity while the CTD determines the bindin

28 stalled replication forks from uncontrolled nucleolytic activity, which otherwise causes irreparable

29 n of TraI that retains the sequence-specific nucleolytic activity.

30 sensitive known substrate for detecting ribo-nucleolytic activity.

31 bute substantially to competence-independent nucleolytic activity.

32 The CTD does not display any nucleolytic activity; however, it substantially increase

33 in which Ku protects chromosome termini from nucleolytic and recombinational activities but is not in

34 ty that Ku protects chromosomal termini from nucleolytic attack and functions as part of a telomeric

35 and U1.2) become reproducibly protected from nucleolytic attack by radicals.

36 important for positioning the nucleotide for nucleolytic attack in the nucleotidyl transfer reaction.

37 shield the natural ends of chromosomes from nucleolytic attack, recognition as double-strand breaks,

38 1 and Ten1, it protects chromosome ends from nucleolytic attack, thus contributing to genome integrit

39 protects stressed DNA replication forks from nucleolytic attack.

40 and plays a critical role in enabling TREX1 nucleolytic attack.

41 essed DNA replication forks against spurious nucleolytic attrition.

42 g, which corresponded to a trypsin-sensitive nucleolytic band of the same size.

43 y bends its DNA substrate using a bipartite, nucleolytic center formed at an N-terminal dimerization

44 DNA duplex that tracks through the enzyme's nucleolytic center.

45 DNA ends followed by 3'-->5' nicking by two nucleolytic centres within a single nuclease molecule th

46 enhances pausing by Pol I and inhibits Pol I nucleolytic cleavage activity.

47 indicating susceptibility of nascent DNA to nucleolytic cleavage and resection when SNF2L is removed

48 avage by tRNA and protection of tRNA against nucleolytic cleavage by MTF.

49 in a specific DNA sequence protects DNA from nucleolytic cleavage by restriction enzymes recognizing

50 y RET1-catalyzed uridylylation, may direct a nucleolytic cleavage of multicistronic precursors.

51 ndonuclease subsequently induces large-scale nucleolytic cleavage of persistent DNA replication inter

52 plication-associated DNA damage arising from nucleolytic cleavage of stalled replication forks.

53 he two MUS81 complexes may promote different nucleolytic cleavage reactions in vivo.

54 mode compared to the product of the initial nucleolytic cleavage.

55 e site, are accessible and can be removed by nucleolytic cleavage.

56 almost all eukaryotic RNAs are generated by nucleolytic cleavage.

57 ge helix, which acts as a pivot, facilitates nucleolytic cleavage.

58 In yeast, resection is carried out by three nucleolytic complexes: Mre11-Rad50-Xrs2, which functions

59 This protein protects ssDNA from nucleolytic damage, prevents hairpin formation and block

60 Following deadenylation, nucleolytic decay of the 3'-UTR occurs generating 3' dec

61 Since the DSB ends undergo nucleolytic degradation (resection) of their 5'-ending s

62 s with BRCA2 and RAD51 to protect forks from nucleolytic degradation and (ii) it recruits the BLM hel

63 oteins protect the chromosomal terminus from nucleolytic degradation and end-to-end fusion, and they

64 ty in genetic crosses but loss of detectable nucleolytic degradation as judged by the growth of mutan

65 ks generated by the fork remodeler HLTF from nucleolytic degradation by MRE11 and CtIP.

66 he BLM helicase, while protecting forks from nucleolytic degradation by MRE11.

67 Here we report that BRCA proteins prevent nucleolytic degradation by protecting replication forks

68 ty to protect stalled replication forks from nucleolytic degradation drives genome instability and un

69 ed to protect stalled replication forks from nucleolytic degradation during response to hydroxyurea (

70 atural or artificial chromosomal ends, or by nucleolytic degradation from induced breaks, or nicks.

71 Chi-containing strand at Chi or switching of nucleolytic degradation from the Chi-containing strand t

72 ctopic sites of microhomology and implicated nucleolytic degradation in illegitimate DSB repair in T4

73 J when Ku70/80 is absent and are attacked by nucleolytic degradation in the absence of 53BP1.

74 HR requires nucleolytic degradation of 5' DNA ends to generate tract

75 if product is made due to template-dependent nucleolytic degradation of an internally quenched probe

76 regulator of genome integrity that restrains nucleolytic degradation of damaged replication forks.

77 preceded by DNA end resection, the 5' to 3' nucleolytic degradation of DNA away from the DSB.

78 Nucleolytic degradation of DNA by Exo1 is inhibited by t

79 ay maintains genome stability by suppressing nucleolytic degradation of DNA ends at double-strand bre

80 pathway selection is the initiation of 5' 3' nucleolytic degradation of DNA ends, a process referred

81 We conclude that RecBCD enzyme's nucleolytic degradation of DNA is not necessary for intr

82 ally relies on sequence-specific binding and nucleolytic degradation of foreign genetic material.

83 ults in DNA2-dependent but MRE11-independent nucleolytic degradation of nascent DNA at stalled replic

84 talled forks results in severe FAN1-mediated nucleolytic degradation of nascent DNA strands.

85 acting at stalled forks with a focus on the nucleolytic degradation of nascent DNA, a process common

86 mbination relies on an initial MRN-dependent nucleolytic degradation of one strand at DNA ends.

87 n of a subset of variants led to deleterious nucleolytic degradation of stalled DNA replication forks

88 g to robust checkpoint signalling and slower nucleolytic degradation of the 5' strand.

89 (DSB) repair, relies on DNA end resection by nucleolytic degradation of the 5'-terminated ends.

90 aks by homologous recombination commences by nucleolytic degradation of the 5'-terminated strand of t

91 by the addition of cycloheximide, slows the nucleolytic degradation of the OLE1 mRNA and blocks the

92 chromatin leading to rapid fork stalling and nucleolytic degradation of unprotected forks by MRE11, r

93 Unlike Pds5, BRCA2 protects forks from nucleolytic degradation only in the presence of genotoxi

94 rotects the 5' terminal viral sequences from nucleolytic degradation or from inducing innate immune r

95 of double-strand breaks (DSBs) undergo 5'-3' nucleolytic degradation to generate single-stranded DNA,

96 cleotides that exhibit partial resistance to nucleolytic degradation was dependent on both oligonucle

97 d within a substrate DNA strand impedes Exo1 nucleolytic degradation, and a 5'-terminal abasic residu

98 BRCA proteins protect reversed forks from nucleolytic degradation, and their loss leads to chemose

99 (ss) DNA on both strands that are exposed to nucleolytic degradation, potentially compromising genome

100 and protects stalled replication forks from nucleolytic degradation, thus representing an attractive

101 se (TERT/EST2) can protect telomeres against nucleolytic degradation.

102 structure, which protects the products from nucleolytic degradation.

103 by protecting stalled replication forks from nucleolytic degradation.

104 RCA2 protects stalled replication forks from nucleolytic degradation.

105 RAD51 protect these structures from extended nucleolytic degradation.

106 hat the nucleic acids are stabilized against nucleolytic degradation.

107 horten due to incomplete DNA replication and nucleolytic degradation.

108 fail to protect their RNA/DNA contents from nucleolytic destruction.

109 However, they typically suffer from nucleolytic digestion and fast clearance from the bloods

110 e-stranded DNA strands, protecting them from nucleolytic digestion and unauthorized transactions.

111 expectedly, stalled forks are susceptible to nucleolytic digestion during late replication resulting

112 homologous recombination requires extensive nucleolytic digestion of DNA ends in a process known as

113 t the Mre11 nuclease complex may mediate the nucleolytic digestion of the 5' strand at secondary stru

114 r, reversed forks are in turn susceptible to nucleolytic digestion of the regressed nascent DNA arms

115 e benefits from both strand displacement and nucleolytic digestion, thus providing guidance for the d

116 nulus of NurA, leading to duplex melting and nucleolytic digestion.

117 Herein, we chemically evolved the nucleolytic DNA enzyme (DNAzyme) 10-23, by incorporating

118 odified Cas9 containing only a single active nucleolytic domain (nCas9).

119 duce a foreshortened protein lacking a RuvCI nucleolytic domain, effectively a 'nickase'.

120 The roles of these proteins in nucleolytic DSB resection are well characterized, but th

121 are coding joints and ends demonstrates that nucleolytic end processing is dramatically reduced in jo

122 for a direct interaction of BLM with a human nucleolytic enzyme.

123 de novo synthesis and secretion of a set of nucleolytic enzymes for scavenging phosphate from extrac

124 activities of phosphate starvation-inducible nucleolytic enzymes, including ribonuclease, phosphodies

125 sting that there may be other RNA-guided non-nucleolytic enzymes.

126 at RNA decay can be triggered by a prior non-nucleolytic event that marks transcripts for rapid turno

127 thin the coding region of an mRNA can effect nucleolytic events that occur at both the 5'- and 3'-end

128 d MSH3 (forming MutSbeta), which can inhibit nucleolytic excision of CAG slip-outs by FAN1.

129 revent mismatch incorporation or promote its nucleolytic excision.

130 namically regulated and how tRNAs (and their nucleolytic fragments) are centrally involved in stress

131 This occurs through a non-nucleolytic function of MRE11 that is important for R-lo

132 ription factors, by inhibiting miR171-guided nucleolytic function.

133 repair of deaminated base damage by making a nucleolytic incision one nucleotide away from the 3' sid

134 ree critical DNA repair processes, including nucleolytic incision, translesion DNA synthesis (TLS), a

135 emoval of FANCD2 from extracts inhibits both nucleolytic incisions near the ICL and translesion DNA s

136 ir, cross-links are resolved ("unhooked") by nucleolytic incisions surrounding the lesion.

137 h unhooks the interstrand cross-link through nucleolytic incisions.

138 Thus, BRCA2 prevents rather than repairs nucleolytic lesions at stalled replication forks to main

139 sm of SAMHD1 restriction and argue against a nucleolytic mechanism, which would not be reversible.

140 of damage to coordinate repair that includes nucleolytic modification of the DNA surrounding the lesi

141 ear the 3' end of truncated mRNA; therefore, nucleolytic mRNA processing is required before paused ri

142 downregulation of Reg-1 in glioma cells in a nucleolytic NYN/PIN domain-dependent manner.

143 airpin-sealed DNA (coding) ends that require nucleolytic opening before their repair by classical non

144 istinguish among several recombinational and nucleolytic pathways, we developed a novel physical assa

145 The DDC mediator 53BP1/Rad9 limits the nucleolytic processing (resection) of a DSB, controlling

146 ted constructs, we demonstrate that both the nucleolytic processing and the ligation of Okazaki fragm

147 ATP-dependent DNA end recognition and nucleolytic processing are central functions of the Mre1

148 Increased nucleolytic processing by deletion of Rad9, the ortholog

149 Therefore, nucleolytic processing by Mre11 is an essential function

150 e-like structural intermediate that requires nucleolytic processing by SbcC-SbcD and ExoI before chro

151 sis and kataegis arise from a combination of nucleolytic processing by TREX1 and cytosine editing by

152 the roles of Mre11 in both DNA bridging and nucleolytic processing during initiation of DSB repair,

153 both facilitated diffusion on chromatin and nucleolytic processing of a DNA substrate.

154 Biogenesis of miRNAs involves nucleolytic processing of a precursor transcript with ex

155 tranded DNA (ssDNA) that is derived from the nucleolytic processing of a primary lesion.

156 DNA resection-the nucleolytic processing of broken DNA ends-is the first s

157 Nucleolytic processing of chromosomal DNA is required in

158 Nucleolytic processing of DNA double-strand breaks (DSBs

159 e RAD50 and MRE11 genes are required for the nucleolytic processing of DNA double-strand breaks.

160 g conserved protein factors that mediate the nucleolytic processing of DNA ends in preparation for ho

161 While RIF1/shieldin blocks 5'-3' nucleolytic processing of DNA ends, it remains unclear h

162 ng DNA ends and ATM activation, but prevents nucleolytic processing of DNA ends, while ATP hydrolysis

163 ling complex DNA end structures by localised nucleolytic processing of DNA termini in preparation for

164 in 1 (53BP1) protects the genome by limiting nucleolytic processing of DSBs by a mechanism that requi

165 lex nicks 5'-terminated DSB ends to initiate nucleolytic processing of DSBs for repair by homologous

166 , RET1's TUTase activity is required for the nucleolytic processing of gRNA, rRNA, and mRNA precursor

167 ANCD2 monoubiquitination to determine if the nucleolytic processing of ICLs is required for the activ

168 uggest that polyadenylation occurs after the nucleolytic processing of primary transcripts and in som

169 Here, we describe a multi-step nucleolytic processing of replication stress-induced ssD

170 s occurs as a consequence of MRE11-dependent nucleolytic processing of reversed forks generated by fo

171 and that this may involve, at least in part, nucleolytic processing of telomeric G4.

172 ster chromatid exchange, both arising out of nucleolytic processing of telomeric homologous recombina

173 group D2 (FANCD2) for the initiation of the nucleolytic processing of the DNA cross-links and stabil

174 BLM is involved in the nucleolytic processing of the ends of DNA double-strand

175 We propose that nucleolytic processing of unusual replication intermedia

176 is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repai

177 uggest that the NHEJ alignment step precedes nucleolytic processing steps in a significant fraction o

178 combination reaction is composed of multiple nucleolytic processing steps mediated by the recombinati

179 spontaneous fork reversal and regulates fork nucleolytic processing thereby promoting sensitivity to

180 staggered in the T tract and is followed by nucleolytic processing to generate the mature 3' end.

181 only at coding ends that have not undergone nucleolytic processing, this observation being the basis

182 ay have previously unrecognized roles in DNA nucleolytic processing.

183 epeats can occur at ends that have undergone nucleolytic processing.

184 esults from lagging strand DNA synthesis and nucleolytic processing.

185 BRCA1 protects single-stranded DNA gaps from nucleolytic processing.

186 were found at these junctions, demonstrating nucleolytic procession of the viral DNA; however, the la

187 blunt ends can be reconciled with the other nucleolytic properties of both Artemis and Artemis.DNA-P

188 For this reason, we further studied the nucleolytic properties of PALF, and we searched for any

189 rocessed by the Mre11-Rad50-Nbs1(Xrs2) (MRN) nucleolytic protein complex in association with the Tel1

190 or other DNA adducts via an Mre11-dependent nucleolytic reaction.

191 , purified RecBCD enzyme has two alternative nucleolytic reactions, depending on the reaction conditi

192 eaving engineered synthetic ribozymes as RNA nucleolytic reagents and as subjects for enzyme kinetics

193 on complexes, patrolling for those requiring nucleolytic rescue, and its short residence time minimiz

194 Nucleolytic resection of DNA double-strand breaks (DSBs)

195 Nucleolytic resection of DNA double-strand breaks is the

196 Nucleolytic resection of DNA ends is critical for homolo

197 ng this process, BRCA1-BARD1 facilitates the nucleolytic resection of DNA ends to generate a single-s

198 r suppressor proteins to mediate the initial nucleolytic resection of DNA lesions and the recruitment

199 e show that RecJ nuclease alone can initiate nucleolytic resection of DNA with 5'-ssDNA overhangs, an

200 coupling factor between presynapsis and the nucleolytic resection of double-stranded DNA ends.

201 The nucleolytic resection of DSB-adjacent DNA is a key step

202 Nucleolytic resection of DSBs generates long 3' single-s

203 donuclease 2) nuclease participates in 3'-5' nucleolytic resection of oxidative DNA damage and activa

204 pair by homologous recombination begins with nucleolytic resection of the 5' DNA strand at the break

205 k repair by homologous recombination entails nucleolytic resection of the 5' strand at break ends.

206 n contrast, homologous recombination entails nucleolytic resection of the 5'-strands, forming 3'-ssDN

207 nclude all of the features observed in vivo (nucleolytic resection, P nucleotides, and N nucleotide a

208 proteins have been performed to evaluate the nucleolytic resection, polymerization, and ligation step

209 shields double-strand DNA breaks (DSBs) from nucleolytic resection.

210 esting a requirement of the SLX4 complex for nucleolytic resolution of branched intermediates during

211 y step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate

212 The possible role of nucleolytic retraction in disengaging pol III from TFIII

213 The VS nucleolytic ribozyme has a core comprising five helices

214 The twister RNA is a recently discovered nucleolytic ribozyme that is present in both bacteria an

215 Ribonucleases and small nucleolytic ribozymes are both able to catalyze RNA stra

216 Nucleolytic ribozymes catalyze site-specific cleavage of

217 Small self-cleaving nucleolytic ribozymes contain catalytic domains that acc

218 dly, this brings us to the four latest small nucleolytic ribozymes termed twister, twister-sister, pi

219 own mechanistic strategies employed by small nucleolytic ribozymes.

220 ibozymes are an extensively studied class of nucleolytic RNAs.

221 esis of primary piRNAs involves at least two nucleolytic steps.

222 intermediates that are subject to additional nucleolytic trimming to render mature crRNAs of specific