ライフサイエンスコーパス: nucleotide incorporation

1 a coli RNAP without altering the fidelity of nucleotide incorporation.

2 ange, which then limited the rate of correct nucleotide incorporation.

3 ll nuclear antigen to achieve rapid rates of nucleotide incorporation.

4 longation complexes after multiple rounds of nucleotide incorporation.

5 TPs to differentiate between right and wrong nucleotide incorporation.

6 ir interactions that lead to highly accurate nucleotide incorporation.

7 ter bond formation in the enzymatic cycle of nucleotide incorporation.

8 ate mechanisms of preserving fidelity during nucleotide incorporation.

9 cence transition matching the rate of single-nucleotide incorporation.

10 imics the state of the RTIC before the first nucleotide incorporation.

11 gh a dramatic decrease in the K(m) value for nucleotide incorporation.

12 ided by the structures of the four states of nucleotide incorporation.

13 hange that precedes the chemical reaction of nucleotide incorporation.

14 has little impact on the kinetics of single-nucleotide incorporation.

15 ect either the efficiency or the fidelity of nucleotide incorporation.

16 e enzyme into position for the next round of nucleotide incorporation.

17 es before and after the chemical reaction of nucleotide incorporation.

18 ream DNA sequences on the kinetics of single nucleotide incorporation.

19 steric requirements on the base-pair during nucleotide incorporation.

20 sphate moiety of the incoming nucleotide, in nucleotide incorporation.

21 ese enzymes to utilize similar mechanisms of nucleotide incorporation.

22 mplex formation from those that occur during nucleotide incorporation.

23 t has been proposed to move at each cycle of nucleotide incorporation.

24 alent metal ion for DNA polymerase-catalyzed nucleotide incorporation.

25 that occur early in the pathway for correct nucleotide incorporation.

26 RNA template, and even perform non-templated nucleotide incorporation.

27 icase DNA unwinding activity, and polymerase nucleotide incorporation.

28 tacking interactions at this position during nucleotide incorporation.

29 ted NTPs, to this site increases the rate of nucleotide incorporation.

30 nd the primer terminus to catalyze efficient nucleotide incorporation.

31 nal changes in RNA polymerase that result in nucleotide incorporation.

32 as incapable of supporting 3D(pol)-catalyzed nucleotide incorporation.

33 efficiency of primer utilization and that of nucleotide incorporation.

34 of an insertion complex capable of accurate nucleotide incorporation.

35 a pyrophosphate ion is generated after each nucleotide incorporation.

36 eaving group affects cognate and non-cognate nucleotide incorporation.

37 ase pair hydrogen bonding recognition during nucleotide incorporation.

38 ring the rate of correct (but not incorrect) nucleotide incorporation.

39 complished only following error-prone purine nucleotide incorporation.

40 DNA polymerases during substrate binding and nucleotide incorporation.

41 to heat-annealed primer-template with single-nucleotide incorporation.

42 ved 10(2)-10(3)-fold decrease in the rate of nucleotide incorporation.

43 e the role of the main channel in regulating nucleotide incorporation.

44 ear to be the preeminent factors controlling nucleotide incorporation.

45 hannel that is involved in the regulation of nucleotide incorporation.

46 crete steps to values consistent with single-nucleotide incorporations.

47 DNA) saturation kinetics for all 16 possible nucleotide incorporations.

48 ve sites simultaneously, although the single nucleotide incorporation (105 s(-1)) was approximately 5

49 e the specificity constant governing correct nucleotide incorporation 150- and 70-fold, respectively,

50 e pre-steady-state rate constant for correct nucleotide incorporation (150 s(-1)) nor on the primary

51 mechanisms: 1) high selectivity for correct nucleotide incorporation, 2) a slowing down of the repli

52 n the DNA helix, where it effectively blocks nucleotide incorporation across the adduct by Dpo4.

53 idues of the NLS in nuclear localization and nucleotide incorporation activities of 3D(pol) We identi

54 Polbeta mouse tissue and KO cells had lower nucleotide incorporation activity.

55 ate than that predicted from the fidelity of nucleotide incorporation alone.

56 ate than that predicted from the fidelity of nucleotide incorporation alone.

57 iption rates, and the kinetics of initiating nucleotide incorporation among the promoters found in th

58 the polymerase active site, one that permits nucleotide incorporation and another that blocks the RNA

59 t conformational flexibility plays a role in nucleotide incorporation and bypass fidelity opposite (+

60 s have helped define individual steps during nucleotide incorporation and conformational changes that

61 The data for both nucleotide incorporation and excision are used to propos

62 re-steady-state and steady-state kinetics of nucleotide incorporation and excision were used to asses

63 ere we report a detailed kinetic analysis of nucleotide incorporation and exonuclease activity for a

64 Nucleotide incorporation and extension opposite N2-ethyl

65 standard methodologies employing radioactive nucleotide incorporation and gel electrophoresis while o

66 Imaging of fluorescent nucleotide incorporation and GFP-PCNA gave exquisite tim

67 polymerase active site poised for efficient nucleotide incorporation and illustrate how DNA polymera

68 ts of fidelity affected by the exo activity, nucleotide incorporation and mismatch extension frequenc

69 the negative effects of the K65R mutation on nucleotide incorporation and on excision.

70 Here, kinetic parameters governing nucleotide incorporation and PPi release were determined

71 ajor groove gamma-HOPdA adducts using single nucleotide incorporation and primer extension analyses.

72 out the role of sugar modifications for both nucleotide incorporation and primer extension.

73 exes along proposed catalytic pathways for L-nucleotide incorporation and provide the structural basi

74 s were studied to evaluate their kinetics of nucleotide incorporation and removal.

75 Single nucleotide incorporation and RNase H cleavage were exami

76 sequencing methods were also used to monitor nucleotide incorporation and subsequent extension by Fam

77 owing open complex formation such as initial nucleotide incorporation and subsequent promoter escape.

78 ut the mechanism of HCV polymerase-catalyzed nucleotide incorporation and the individual steps employ

79 while differing in relative rates of single-nucleotide incorporation and the putative conformational

80 ound to a lesion, has a strong commitment to nucleotide incorporation and thus repair.

81 Using kinetic measurements of nucleotide incorporations and a fidelity assay with gapp

82 correct nucleotide incorporation, incorrect nucleotide incorporation, and ribonucleotide incorporati

83 r appears as the sole rate-limiting step for nucleotide incorporation, and the rate of phosphoryl tra

84 as rate-limiting so that the average rate of nucleotide incorporation ( approximately 28 s(-1)) was c

85 mations adopted during correct and incorrect nucleotide incorporation are distinct.

86 The steps employed during a single cycle of nucleotide incorporation are identical to those employed

87 Further nucleotide incorporation assays by HCV NS5B RNA-dependen

88 Single nucleotide incorporation assays have been used to probe

89 minators for genotype 1b HCV-pol, and single nucleotide incorporation assays revealed that 2'-C-Me-DA

90 Furthermore, pre-steady-state nucleotide incorporation assays revealed that polD preve

91 lassical molecular dynamics simulations, and nucleotide incorporation assays to investigate the mecha

92 Pre-steady-state single-turnover nucleotide incorporation assays were performed to obtain

93 , processive elongation activity, and single nucleotide incorporation assays.

94 only a large, fast phase was observed during nucleotide incorporation at non-pause sites.

95 at concomitantly increases the efficiency of nucleotide incorporation at normal and transiently slipp

96 ing the elusive transient intermediates, for nucleotide incorporation at the template/primer DNA junc

97 the presence of a DNA trap, the kinetics of nucleotide incorporation at these sites was biphasic in

98 protein conformational change limits correct nucleotide incorporations at 56 degrees C.

99 ion to Eco RNAP, Mtb displays slower initial nucleotide incorporation but faster overall promoter esc

100 platin-d(GpG) adduct affected six downstream nucleotide incorporations, but interestingly the fidelit

101 ied DNA, increased pre-steady-state rates of nucleotide incorporation by >2 orders of magnitude, and

102 The observed rate constant for nucleotide incorporation by 3D(pol) (86 s(-1)) is dictat

103 The observed rate for single nucleotide incorporation by a preformed DNA-Pol delta co

104 While matched nucleotide incorporation by DNA polymerase beta (Pol bet

105 ability of this mutant protein to stimulate nucleotide incorporation by DNA polymerase eta (pol eta)

106 However, the fidelity of nucleotide incorporation by DNA polymerase iota opposite

107 ate genomic information.1-3 The mechanism of nucleotide incorporation by DNA polymerases has been ext

108 Two divalent metal ions are required for nucleotide incorporation by DNA polymerases.

109 DNA replication achieve high processivity of nucleotide incorporation by forming a complex with proce

110 The efficiency and fidelity of nucleotide incorporation by high-fidelity replicative DN

111 For G[8,5-Me]T, nucleotide incorporation by hpol eta was significantly d

112 DNA, the efficiency (V(max)/K(m)) of correct nucleotide incorporation by hPoliota is increased approx

113 The efficiency (V(max)/K(m)) of correct nucleotide incorporation by hPolkappa is enhanced approx

114 rect sequence of post-chemistry steps during nucleotide incorporation by human DNA polymerase beta (h

115 fficient bypass of these lesions may require nucleotide incorporation by other DNA polymerases follow

116 WRN increases the rate of nucleotide incorporation by pol delta in the absence of

117 Steady-state kinetic analyses for nucleotide incorporation by pol eta showed that the 3'-c

118 hes present only a modest kinetic barrier to nucleotide incorporation by pol kappa.

119 ate kinetics to investigate the mechanism of nucleotide incorporation by Poleta and show that it util

120 we found that the efficiency and accuracy of nucleotide incorporation by Poleta are severely impaired

121 ere, we use steady-state kinetics to examine nucleotide incorporation by Rev1 opposite undamaged and

122 hanistic impact of protein-template-directed nucleotide incorporation by Rev1p.

123 Steady-state kinetics of single nucleotide incorporation by RT and T7(-) showed a decrea

124 y accurate, with a higher fidelity of single nucleotide incorporation by the active site than that of

125 Nucleotide incorporation by the herpes simplex virus typ

126 ffector that increases the intrinsic rate of nucleotide incorporation by the polymerase.

127 Steady-state kinetic analyses for nucleotide incorporation by yeast pol eta showed that th

128 oles of Mg(2+) by kinetic analysis of single nucleotide incorporation catalyzed by HIV reverse transc

129 s of kinetic evidence suggested that correct nucleotide incorporation catalyzed by PolB1 exo- was lim

130 nucleotide to examine the kinetics of single nucleotide incorporation catalyzed by recombinant human

131 KM-1 on the parameters governing the single nucleotide incorporation catalyzed by RT.

132 d the complete kinetic mechanism for correct nucleotide incorporation catalyzed by the RNA-dependent

133 ed the complete kinetic mechanism for single nucleotide incorporation catalyzed by the RNA-dependent

134 led studies of the kinetics and mechanism of nucleotide incorporation catalyzed by the RNA-dependent

135 Consequently, the erroneous nucleotide incorporations catalyzed by DNA polymerases l

136 ing to the decrease in the rate of incorrect nucleotide incorporation compared with correct insertion

137 This specificity of nucleotide incorporation correlates well with the known

138 In general, the efficiency of nucleotide incorporation does not depend on the hydrogen

139 about the reaction pathway of NS5B-catalyzed nucleotide incorporation due to the lack of a kinetic sy

140 have dissected the kinetic pathway of single nucleotide incorporation during elongation.

141 ns across the chromosome by mapping sites of nucleotide incorporation during hydroxyurea arrest.

142 hundreds of germ-line gene segments, random nucleotide incorporation during joining of gene segments

143 The kinetics of multiple nucleotide incorporation during processive elongation al

144 Nucleotide incorporation during transcription by RNA pol

145 r measuring the real-time kinetics of single-nucleotide incorporation during transcription elongation

146 its 5'-phosphate were both found to increase nucleotide incorporation efficiency (kp/Kd) by 15 and 11

147 Both nucleotide incorporation efficiency and fidelity decreas

148 The single nucleotide incorporation efficiency of the altered nucle

149 Although the small subunits enhanced correct nucleotide incorporation efficiency, there was a wide ra

150 ses were because of a 1,000-fold decrease in nucleotide incorporation efficiency.

151 is characterized by a strong stimulation in nucleotide incorporation either directly opposite a lesi

152 s a critical role in prevention or repair of nucleotide incorporation errors during genome replicatio

153 with a frequency from 10 to 80% during each nucleotide incorporation event.

154 ariability with the resolution of individual nucleotide incorporation events.

155 d tracking) of RNAP along DNA between single-nucleotide incorporation events.

156 Single nucleotide incorporation experiments indicated that alth

157 Single nucleotide incorporation experiments indicated that Kf e

158 The collective data set encompassing nucleotide incorporation, extension, and excision is use

159 ic assays were employed to determine the low nucleotide incorporation fidelity and establish a minima

160 s of motif D can be targeted when changes in nucleotide incorporation fidelity are desired.

161 Using NMR, we show that this change in nucleotide incorporation fidelity correlates with a chan

162 The nucleotide incorporation fidelity of the viral RNA-depen

163 al867 hTERT mutants also displayed increased nucleotide incorporation fidelity, implicating Val867 as

164 Nucleotide incorporation fidelity, mismatch extension, a

165 time-resolved experiment monitoring a single-nucleotide incorporation followed by primer extension by

166 sequence context indicated that the rate of nucleotide incorporation followed the order: dAMP > dGMP

167 We examined the rate of single-nucleotide incorporation for all four tNTPs and dNTPs fr

168 e rate-limiting step in the overall cycle of nucleotide incorporation for the labeled KF-DNA system.

169 e conformational dynamics during the correct nucleotide incorporation forward and reverse reactions u

170 cosity, we have decoupled the rate of single-nucleotide incorporation from the rate of the slow fluor

171 nly change observed at the rate expected for nucleotide incorporation had a very small amplitude, sug

172 different from those observed during correct nucleotide incorporation, implying that the conformation

173 elongation, as determined by pulse-labeling nucleotide incorporation in replication foci and DNA fib

174 m-Tipin-deficient cells completely abrogates nucleotide incorporation in S phase, indicating that the

175 tic data for the chemistry step with correct nucleotide incorporation in T7 DNA polymerase.

176 phage T7 RNA polymerase (RNAP), which allows nucleotide incorporation in the growing RNA with the sel

177 sts that these residues are also crucial for nucleotide incorporation in the other members of the fam

178 The rate-limiting step for nucleotide incorporation in the pre-steady state for mos

179 seven was observed on the rate constant for nucleotide incorporation in the pre-steady state; none w

180 ) to 10(-8), or one error per 10(6) to 10(8) nucleotide incorporations in vivo.

181 kinetic parameters, kpol and Kd, for correct nucleotide incorporation, incorrect nucleotide incorpora

182 Steady-state kinetic analysis of single nucleotide incorporation indicates that dCMP is most fre

183 The Polmu specificity of nucleotide incorporation indicates that the deletion res

184 The kinetics of nucleotide incorporation into 24/36-mer primer/template

185 The fidelity of correct nucleotide incorporation into damaged DNA is essential f

186 all phosphorothioate effect of 2 for correct nucleotide incorporation into DNA by pol delta.PCNA indi

187 By measuring nucleotide incorporation into HCV genomes, we found that

188 directly measure the kinetics of single-base nucleotide incorporation into primed DNA templates coval

189 the rate of noncomplementary or illegitimate nucleotide incorporation into the palindrome.

190 Relative to nucleotide incorporation into undamaged DNA, three of th

191 Pyrophosphate ion (PP(i)) release after nucleotide incorporation is a necessary step for RNA pol

192 orrectness of the bound nucleotide, faithful nucleotide incorporation is achieved.

193 These data indicate that nucleotide incorporation is an early and critical event

194 In this system nucleotide incorporation is dependent on the HSV-1 uraci

195 Nucleotide incorporation is hindered because key residue

196 fter nucleotide binding and fingers-closing, nucleotide incorporation is overwhelmingly likely to occ

197 thionine, conditions under which the rate of nucleotide incorporation is reduced, we observe a signif

198 te of P-O bond cleavage and formation during nucleotide incorporation is still unclear.

199 Correct nucleotide incorporation kinetics revealed a relatively

200 examine DNA polymerase activities including nucleotide incorporation kinetics, strand displacement s

201 ng was substantially faster than the rate of nucleotide incorporation measured in chemical quench exp

202 riments, closely matched the rate of correct nucleotide incorporation, measured in rapid quench-flow

203 state kinetic approaches to characterize the nucleotide incorporation mechanism of Pol I.

204 Two different nucleotide incorporation methods were used to evaluate r

205 ing the template position in the DNA impacts nucleotide incorporation more at the nucleotide-binding

206 ir of damaged bases and AP sites involving 1-nucleotide incorporation, named single nucleotide (SN)-B

207 s nucleotides, and a 'closed' state in which nucleotide incorporation occurs.

208 We evaluated the impact of (S)- or (R)-GNA nucleotide incorporation on RNA duplex structure by dete

209 ymidine glycol does not significantly affect nucleotide incorporation opposite 2-deoxyribonolactone i

210 was found to be a more essential factor for nucleotide incorporation opposite 8-oxoG adducts than un

211 kinetic analyses to examine the mechanism of nucleotide incorporation opposite a cis-syn thymine-thym

212 thymine, whereas its efficiency for correct nucleotide incorporation opposite a template guanine or

213 First, Polzeta catalyzes nucleotide incorporation opposite AAF-guanine and TT (6-

214 ntroduced into B7528 or its derivatives, and nucleotide incorporation opposite abasic sites was analy

215 ly, the data indicate that the efficiency of nucleotide incorporation opposite an abasic site is cont

216 Poleta displayed "burst" kinetics for nucleotide incorporation opposite both the damaged and n

217 ted to catalyze stable, yet often erroneous, nucleotide incorporation opposite damaged template bases

218 ly than G3*:C pair, but it is inefficient at nucleotide incorporation opposite dG-C8-IQ.

219 To understand the mechanisms of nucleotide incorporation opposite different template bas

220 ly equivalent efficiencies and fidelities of nucleotide incorporation opposite each of the four templ

221 ly equivalent efficiencies and fidelities of nucleotide incorporation opposite each of the four templ

222 mine the mechanisms of correct and incorrect nucleotide incorporation opposite G and 8-oxoG by Saccha

223 Nucleotide incorporation opposite G-AF is achieved in so

224 vides a facile way to quantify the extent of nucleotide incorporation opposite non-instructional DNA

225 observations, we conclude that polbeta slows nucleotide incorporation opposite O6MeG by inducing an a

226 air, contribute to the efficiency of correct nucleotide incorporation opposite template purines by Po

227 ied out pre-steady-state kinetic analyses of nucleotide incorporation opposite templates A and T.

228 tential role of pi-electron surface area for nucleotide incorporation opposite templating and nontemp

229 Interestingly, nucleotide incorporation opposite the (R)-butadiene mono

230 Steady-state kinetic measurements for nucleotide incorporation opposite the 3'-cytosine of the

231 PCNA, RFC, and RPA also stimulate nucleotide incorporation opposite the 3'-T of the (6) th

232 ion, but with markedly reduced efficiency in nucleotide incorporation opposite the 5'-guanine of the

233 to a high fidelity DNA polymerase than does nucleotide incorporation opposite the adduct because the

234 structure-function relationship involved in nucleotide incorporation opposite the bulky 10S (+)-tran

235 n that poliota is also capable of error-free nucleotide incorporation opposite the bulky major groove

236 olute block to human RNAP II elongation, and nucleotide incorporation opposite the lesion is not obse

237 ions are consistent with the kcat values for nucleotide incorporation opposite the lesion studied, pr

238 are presented that reveal relatively facile nucleotide incorporation opposite the lesion, but very i

239 s stop precisely at the damaged site without nucleotide incorporation opposite the lesion, while exte

240 We have investigated nucleotide incorporation opposite the major adduct of 2-

241 air, which can contribute to the promiscuous nucleotide incorporation opposite this lesion.

242 with PCNA greatly stimulates its ability for nucleotide incorporation opposite this lesion.

243 e methylation could either retain the normal nucleotide incorporation or completely inhibit the DNA s

244 Therefore, the nucleotide incorporation pattern by hpol eta was not con

245 on from mismatched primer termini and on the nucleotide incorporation pattern was altered upon additi

246 d holoenzyme both selected against incorrect nucleotide incorporation predominantly at the level of n

247 d that the rate-limiting step in the overall nucleotide incorporation process for matched as well as

248 transiently misaligned DNA intermediates and nucleotide incorporation products formed by DNA polymera

249 PCNA enhanced the nucleotide incorporation rate by >10 fold.

250 h the increase procured by the effect on the nucleotide incorporation rate constant kp rather than th

251 (-7)) resulting from large decreases in both nucleotide incorporation rate constants and ground-state

252 site are hypothesized to explain the slower nucleotide incorporation rate of the RTIC.

253 stantially less stimulation of the Pol delta nucleotide incorporation rate, identifying the face of P

254 nd V differ from one another, and Pol II, in nucleotide incorporation rate, transcriptional accuracy

255 While nucleotide incorporation rates (k(pol)) were generally h

256 It possesses both unusually high nucleotide incorporation rates and high-error rates allo

257 ground-state nucleotide binding affinity and nucleotide incorporation rates between correct and incor

258 ctly measuring released hydrogen ions during nucleotide incorporation rather than relying on indirect

259 e collected time courses for single turnover nucleotide incorporation reactions over a range of subst

260 ended in sequential DNA polymerase-catalyzed nucleotide incorporation reactions, each with a single f

261 Moreover, we found that following nucleotide incorporation, Rev1 converts the pyrophosphat

262 a global fit of the data over six sequential nucleotide incorporations revealed that the overall poly

263 single-stranded template beyond the site of nucleotide incorporation, revealing contacts with the te

264 d by our observations that during processive nucleotide incorporation, sequential rounds of RNA cleav

265 ophosphate (PPi) dissociation was fast after nucleotide incorporation so that it did not contribute t

266 and tCTP have comparable rates for the first nucleotide incorporation step (kobs1).

267 eatures that impose high fidelity during the nucleotide-incorporation step and those that accommodate

268 cleotide-binding step, not at the subsequent nucleotide-incorporation step.

269 e at the nucleotide-binding step than at the nucleotide-incorporation step.

270 he human enzyme has a 50-fold-faster rate of nucleotide incorporation than the yeast enzyme but binds

271 is too slow to account for the rapid rate of nucleotide incorporation that occurs during processive t

272 which can bind to the EC but cannot lead to nucleotide incorporation, the analysis of the hyperbolic

273 During processive nucleotide incorporation, the first nucleotide (TTP) was

274 Following nucleotide incorporation, the hydrogen bonds between the

275 also decreased pronouncedly the fidelity of nucleotide incorporation; the insertion of dAMP and dGMP

276 uced both the efficiency and the fidelity of nucleotide incorporation; the insertion of dGMP or dAMP

277 we showed that for correct versus incorrect nucleotide incorporation, there are significant differen

278 As in our studies of nucleotide incorporation, (+)-trans-anti-[BP]-N(2)-dG wa

279 uplex relative to the active site after each nucleotide incorporation (type I or nucleotide addition

280 ured saturation kinetics for all 16 possible nucleotide incorporations under single turnover conditio

281 te kinetic methods to investigate individual nucleotide incorporations upstream, opposite, and downst

282 tion of the catalytic mechanism of incorrect nucleotide incorporation using molecular dynamics simula

283 Time courses in nucleotide incorporation using several DNA substrates we

284 Although the degree of infidelity in nucleotide incorporation varies according to the mispair

285 e residues reduces the efficiency of correct nucleotide incorporation very considerably.

286 ead, PCNA binding improves the efficiency of nucleotide incorporation via a reduction in the apparent

287 ce decrease with a rate equal to the rate of nucleotide incorporation was observed with both 0 and +1

288 the nondamaged template, the rate (kpol) of nucleotide incorporation was the same whether the templa

289 hanism on the thermodynamics and kinetics of nucleotide incorporation, we have carried out pre-steady

290 Pre-steady-state bursts of nucleotide incorporation were observed for pol delta in

291 we examine the effects on Pol eta-catalyzed nucleotide incorporation when 3-deazaguanine, a base ana

292 fined as the ratio of right (R) to wrong (W) nucleotide incorporations when dRTP and dWTP substrates

293 nfers a large reduction in the efficiency of nucleotide incorporation, whereas the remaining five Rad

294 that Rev1p utilizes an unusual mechanism of nucleotide incorporation whereby the template residue is

295 the mutation increases the probability that nucleotide incorporation will occur.

296 erlying mechanism by which PrimPol catalyzes nucleotide incorporation with two common metal cofactors

297 ghts into the effects of the bulky adduct on nucleotide incorporation within the polymerase active si

298 ociated with an ability to induce precocious nucleotide incorporation within the somatic partner nucl

299 Notably, by accelerating the rate of nucleotide incorporation, WRN increases mutagenesis by P

300 retain high-catalytic efficiency for correct nucleotide incorporation, yet have increased error rates