ライフサイエンスコーパス: nucleotidyl

1 These findings highlight nucleotidyl activation as a predominant chemical logic i

2 he B-LNP was encapsulated with diABZI, a non-nucleotidyl agonist for stimulator of interferon genes.

3 Free radical attack on the nucleotidyl C-1' carbon yields 2-deoxyribonolactone (dL)

4 Recent studies have revealed that the nucleotidyl cyclase activity of ExoY is stimulated by ac

5 undling, and indirectly, via actin-activated nucleotidyl cyclase activity.

6 nylyl cyclase, we constructed a model of the nucleotidyl cyclase domain and mutagenized several resid

7 50, which encodes a protein with a class III nucleotidyl cyclase domain, is required for cyclic GMP s

8 volved in blue-light sensing using FAD and a nucleotidyl cyclase domain.

9 ion but also for other members of the larger nucleotidyl cyclase family.

10 Although it is known that ExoY is a soluble nucleotidyl cyclase that increases the cytoplasmic level

11 The promiscuous nucleotidyl cyclase, exoenzyme Y (ExoY), is one of the m

12 a permuted histidine-aspartate domain and a nucleotidyl cyclase-like domain, both of which contain s

13 ultracentrifugation, whereas other class III nucleotidyl cyclases are functional dimers.

14 ations in the corresponding regions of human nucleotidyl cyclases disrupt the normal helical domain s

15 ring, and characterization of photoactivated nucleotidyl cyclases that can be used to manipulate cAMP

16 Nucleotidyl cyclases, including membrane-integral and so

17 own to be involved in defining sub-types for nucleotidyl cyclases, protein kinases, lactate/malate de

18 biochemically distinct from other mammalian nucleotidyl cyclases.

19 NAD+-dependent nucleotidyl diphosphohexose 4,6-dehydratases which trans

20 osphohexose 4,6-dehydratases which transform nucleotidyl diphosphohexoses into corresponding 4-keto-6

21 echanism through the formation of a covalent nucleotidyl-enzyme intermediate and overall retention of

22 a catalytic domain similar to that of other nucleotidyl-glucose pyrophosphorylases with a carboxy-te

23 es as the nucleophilic catalyst to which the nucleotidyl group is bonded covalently in the covalent i

24 ter, thereby eliminating the 3'-terminal TA4 nucleotidyl group.

25 r catalysis, thus unifying the HIT family as nucleotidyl hydrolases, transferases, or both.

26 NSP2 and the histidine triad (HIT) family of nucleotidyl hydrolases, which in turn has suggested the

27 tous cellular histidine triad (HIT) group of nucleotidyl hydrolases.

28 to the nonbridging phosphoryl oxygens of the nucleotidyl intermediate appear crucial for the formatio

29 ate into its cyclic diphosphate proceeds via nucleotidyl intermediates and is catalyzed by the produc

30 hough the product is analogous to the enzyme-nucleotidyl intermediates isolated during the reactions

31 them to interact with substrates containing nucleotidyl moieties.

32 pectively, intact and processed McC with the nucleotidyl moiety.

33 dRp) contains two active sites that catalyze nucleotidyl-monophosphate transfer (NMPylation).

34 ultifunctional RNA-binding NSP2 octamer with nucleotidyl phosphatase activity is central to viroplasm

35 bilizing weak interactions that occur during nucleotidyl-protein-primed initiation events in the vira

36 tral domain that contains the active site of nucleotidyl transfer (Lys-231); (iii) a protease-resista

37 structures illuminate the stereochemistry of nucleotidyl transfer and reveal how remodeling of active

38 vitro by D1(1-545)-K260A, which is inert in nucleotidyl transfer but active in gamma-phosphate cleav

39 ranslocated register allowed NTP binding and nucleotidyl transfer but inhibited pyrophosphorolysis an

40 Nucleotidyl transfer by hRev1 is not necessary for mecha

41 common ancestral mechanism of phosphoryl and nucleotidyl transfer can be harnessed to perform seeming

42 transcript retention, substrate loading, and nucleotidyl transfer catalysis.

43 e mechanism of DNA polymerase beta-catalyzed nucleotidyl transfer consists of chemical steps involvin

44 Here we show that nucleotidyl transfer depends on two ionizable groups wit

45 uld help define the catalytic mechanisms for nucleotidyl transfer during RNA and DNA synthesis and th

46 Neither nucleobase modification nor nucleotidyl transfer has previously been reported for a

47 Non-template-directed nucleotidyl transfer is also observed when pol beta-DNA

48 , one of the three carboxylates required for nucleotidyl transfer is located on a different beta stra

49 CCl2 to explore leaving-group effects on the nucleotidyl transfer mechanism and fidelity of DNA polym

50 g Rh.dTTP opposite dAP, the templating base, nucleotidyl transfer occurred, but the rate of product f

51 Ribozymes can catalyze phosphoryl or nucleotidyl transfer onto ribose hydroxyls of RNA chains

52 e that C-site metal ion binding preceded the nucleotidyl transfer reaction and demonstrate that the C

53 mimic the pyrophosphate leaving group of the nucleotidyl transfer reaction and effectively inhibit RN

54 of DNA polymerases both for catalysis of the nucleotidyl transfer reaction and for base excision.

55 promoter DNA complex crystals to trigger the nucleotidyl transfer reaction and freezing crystals at d

56 Asn564 contact the incoming dNTP during the nucleotidyl transfer reaction as judged by variations in

57 ctural model based on the stereochemistry of nucleotidyl transfer reaction as well as recent crystall

58 The nucleotidyl transfer reaction catalyzed by DNA polymeras

59 rmined the entire free energy profile of the nucleotidyl transfer reaction catalyzed by Pol kappa and

60 unveil the mechanism and free energetics of nucleotidyl transfer reaction in an SNT called GlmU thro

61 ular mechanics calculations for modeling the nucleotidyl transfer reaction in RNase H, clarifying the

62 arried out an extensive investigation of the nucleotidyl transfer reaction mechanism in the well-char

63 tide and a 3' splice site oligonucleotide, a nucleotidyl transfer reaction occurs that mimics the sec

64 gases, play critical roles in the subsequent nucleotidyl transfer reaction that produces the DNA-aden

65 2+) which restored the k(pol) values for the nucleotidyl transfer reaction to near wild-type levels.

66 metal ion to the A site is required for the nucleotidyl transfer reaction to occur, this metal bindi

67 at the catalytic site, thereby allowing the nucleotidyl transfer reaction to take place with little

68 of Arg-61 synergistically contribute to the nucleotidyl transfer reaction, with additional influence

69 the nucleotide for nucleolytic attack in the nucleotidyl transfer reaction.

70 the incoming dNTP of RB69 gp43 prior to the nucleotidyl transfer reaction.

71 of the reactive groups of substrates for the nucleotidyl transfer reaction.

72 ic metal binding is the last step before the nucleotidyl transfer reaction.

73 ement prechemistry step occurring before the nucleotidyl transfer reaction.

74 ates of the ternary EDN complex precedes the nucleotidyl transfer reaction.

75 ate of the dNTP, followed by the associative nucleotidyl transfer reaction; this is facilitated by a

76 isotope effects to investigate mechanisms of nucleotidyl transfer reactions in nucleic acids.

77 ments and the stereochemical course of these nucleotidyl transfer reactions.

78 g features are likely requisite elements for nucleotidyl transfer reactions.

79 -PO4 and 3'-OH polynucleotide ends via three nucleotidyl transfer steps involving ligase-adenylate an

80 ently seals 3'-OH/5'-PO4 RNA nicks via three nucleotidyl transfer steps.

81 i at DNA nicks by means of a series of three nucleotidyl transfer steps.

82 '-OH and 5'-PO(4) ends via a series of three nucleotidyl transfer steps.

83 ia an ATP-dependent pathway comprising three nucleotidyl transfer steps: reaction of Rtc with ATP to

84 ylation reactions follow the same pathway of nucleotidyl transfer through a covalent aprataxin-(His14

85 d Mn2+, X-ray structural analysis shows that nucleotidyl transfer to the primer 3'-OH takes place dir

86 merases, by probing leaving group effects on nucleotidyl transfer using a series of dGTP bisphosphona

87 of triphosphate RNA ends as an acceptor for nucleotidyl transfer when gamma-phosphate cleavage is ra

88 mation of the DNA substrate to orient it for nucleotidyl transfer, although this is not coupled to la

89 lytic residue Asp192, dNTP, and template for nucleotidyl transfer, effectively assembling the active

90 termediate, they illuminate the mechanism of nucleotidyl transfer, especially the stereochemical tran

91 ) complex-highlight a two-metal mechanism of nucleotidyl transfer, whereby (i) an enzyme-bound "catal

92 ive site for the subsequent chemical step of nucleotidyl transfer--in contrast to an opening trend wh

93 only proteins known to catalyze 2'-specific nucleotidyl transfer.

94 sp532, Lys533, and Asn537 in GTP binding and nucleotidyl transfer.

95 synthesis utilizing two metals to facilitate nucleotidyl transfer.

96 active site are not properly positioned for nucleotidyl transfer.

97 of active-site residues in the chemistry of nucleotidyl transfer.

98 /molecular mechanical (QM/MM) studies on the nucleotidyl-transfer reaction catalyzed by the lesion-by

99 The nucleotidyl-transfer reaction coupled with the conformat

100 s of PrimPol are critically dependent on the nucleotidyl-transfer reaction to incorporate deoxyribonu

101 sing substrate and increasing product of the nucleotidyl-transfer reaction.

102 transfers occur in the transition state for nucleotidyl-transfer reactions catalyzed by RB69 DNA-dep

103 ater molecule is the rate-limiting step, the nucleotidyl-transfer step is associative with a metastab

104 The kinase-like nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain of nsp12 in SARS-

105 which resides in a Nidovirus RdRp-Associated Nucleotidyl transferase (NiRAN) domain, is poorly charac

106 strate for Nsp12's Nidovirus RdRp-Associated Nucleotidyl transferase (NiRAN) domain.

107 ition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domain

108 losis (Mt-Lig) possesses a unique variety of nucleotidyl transferase activities, including gap-fillin

109 onspecific and template-independent terminal nucleotidyl transferase activity was observed with the B

110 HESO1 exhibits terminal nucleotidyl transferase activity, prefers uridine as the

111 possesses a template-independent 3'-terminal nucleotidyl transferase activity.

112 Here we report such a non-canonical 3'-5' nucleotidyl transferase activity.

113 R771W mutations, respectively located in the nucleotidyl transferase and oligonucleotide binding doma

114 ted XTUT7 enzyme, which contained solely the nucleotidyl transferase and poly(A) polymerase-associate

115 otype Toprim enzyme that might have had both nucleotidyl transferase and polynucleotide cleaving acti

116 ures reveal a tight docking of the conserved nucleotidyl transferase bi-domain module with a RET1-spe

117 alian nuclear enzyme functions not only as a nucleotidyl transferase but also has a dRP lyase activit

118 The nucleotidyl transferase cGAS, its second-messenger produ

119 complex reveals a unique docking site on the nucleotidyl transferase domain for an 8-amino-acid Pol2

120 cking sites localized to the Cgt1 N-terminal nucleotidyl transferase domain.

121 a), a recently identified, essential nuclear nucleotidyl transferase encoded by two redundant genes,

122 miRNA 3' additions are regulated by multiple nucleotidyl transferase enzymes.

123 e NHEJ activity of Pol4 was dependent on its nucleotidyl transferase function, as well as its unique

124 Here we examined the role of nucleotidyl transferase motif V ((184)LLKMKQFKDAEAT(196)

125 f an N-terminal domain (domain 1, containing nucleotidyl transferase motifs I, III, IIIa and IV) and

126 atalytic residues of Rnl2 are located within nucleotidyl transferase motifs I, IV, and V that are con

127 ctuated by a surface-accessible loop between nucleotidyl transferase motifs III and IIIa.

128 Ceg1p is bound to Cet1p are located between nucleotidyl transferase motifs V and VI.

129 encodes a protein that is classified in the nucleotidyl transferase protein family and was previousl

130 Significantly, the crucial nucleotidyl transferase reaction distance (P(alpha)-O3')

131 DNA polymerases catalyze a metal-dependent nucleotidyl transferase reaction during extension of a D

132 This nucleotidyl transferase reaction required a divalent cat

133 NA capping enzymes are members of a covalent nucleotidyl transferase superfamily defined by a common

134 divergent member of the DNA polymerase beta nucleotidyl transferase superfamily, which includes CCA-

135 nzyme is the smallest member of the covalent nucleotidyl transferase superfamily, which includes the

136 is also the smallest member of the covalent nucleotidyl transferase superfamily, which includes the

137 in the sequence motifs characteristic of the nucleotidyl transferase superfamily.

138 tem that adds CCA to tRNAs in a eukaryote; a nucleotidyl transferase that adds nucleotides to RNA wit

139 HESO1 encodes a terminal nucleotidyl transferase that prefers to add untemplated

140 nucleotide selection by human Rev1, a unique nucleotidyl transferase that uses a protein template-dir

141 rocessing and 3' nucleotide addition by tRNA nucleotidyl transferase to yield a discrete tRNA-sized m

142 abidopsis, HEN1 suppressor 1 (HESO1, a miRNA nucleotidyl transferase) uridylates 5' fragments to trig

143 n and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cy

144 We also observed that suppression of one nucleotidyl transferase, TUT1, resulted in a global decr

145 iac apoptosis was measured by terminal deoxy-nucleotidyl transferase-mediated dUTP nick end-labeling

146 iac apoptosis was measured by terminal deoxy-nucleotidyl transferase-mediated dUTP nick end-labeling

147 Apoptosis was assessed with terminal nucleotidyl transferase-mediated nick end labeling (TUNE

148 antigen and induction of apoptosis (terminal nucleotidyl transferase-mediated nick end labeling and c

149 sessed by Annexin V, caspase-3, and terminal nucleotidyl transferase-mediated nick end labeling) and

150 angiogenesis (CD31), and apoptosis (terminal nucleotidyl transferase-mediated nick end labeling) were

151 randed cDNA product by use of terminal deoxy-nucleotidyl transferase; (iii) ligation of a DNA linker

152 Ribonuclease H (RNase H) belongs to the nucleotidyl-transferase (NT) superfamily and hydrolyzes

153 RNA interference screen identified Terminal Nucleotidyl-transferase 4b (TENT4b/Papd5) as an essentia

154 and a monomer fold common to members of the nucleotidyl-transferase alpha/beta phosphodiesterase sup

155 nylation complex that includes the conserved nucleotidyl-transferase core of GLD-2 and the N-terminal

156 Arg-155, and Ser-170) within the N-terminal nucleotidyl-transferase domain of Rnl2 and impute specif

157 D-2 thus appears to have evolved specialized nucleotidyl-transferase properties that match the 3' end

158 Ribonuclease H (RNase H) belongs to the nucleotidyl-transferase superfamily and hydrolyzes the p

159 RNase H belongs to a nucleotidyl-transferase superfamily, which includes tran

160 onserved domains with sequence similarity to nucleotidyl transferases (NTs) and acyl transferases and

161 Sugar nucleotidyl transferases (SNTs) catalyze nucleotidyltran

162 A substrates for isotope effect studies with nucleotidyl transferases and hydrolases.

163 e implications for the evolution of covalent nucleotidyl transferases and virus-host dynamics based o

164 ion blocking the action of 4'-aminoglycoside nucleotidyl transferases by the use of recombinant E. co

165 The TOPRIM domain found in many nucleotidyl transferases contains a DxD motif involved i

166 es, which comprise a superfamily of covalent nucleotidyl transferases defined by a constellation of c

167 e to screen a panel of eight candidate miRNA nucleotidyl transferases for 3' miRNA modification activ

168 NTA following the suppression of a panel of nucleotidyl transferases in cancer cell lines.

169 onserved motifs that define a superfamily of nucleotidyl transferases that act via enzyme-(lysyl-N)-N

170 ars, the characterization and mutagenesis of nucleotidyl transferases that can recognize and couple s

171 These enzymes, unlike other nucleotidyl transferases, catalyze 2'-5', not 3'-5', pho

172 As nucleotidyl transferases, formation of a covalent enzyme

173 RNA polymerase in the Pol ss superfamily of nucleotidyl transferases, Trf4p, and a zinc knuckle prot

174 alytic mechanism for the 2'- and 3'-specific nucleotidyl transferases.

175 dylation and thus relies on exonucleases and nucleotidyl transferases.

176 otein has domain similarity with other known nucleotidyl transferases.

177 le-domain proteins or fused with the pathway nucleotidyl transferases; the fusion of KDO8PP with the

178 The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the cons

179 The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases], which catalyze synthesis of t

180 ediates of cA6 synthesis suggests a 3'-to-5' nucleotidyl transferring process.

181 t the ribozyme catalyzes both phosphoryl and nucleotidyl transfers.