ライフサイエンスコーパス: oligo(dT)

1 croscopic translocation rates of 3 nt s(-1) (oligo(dT)), 35 nt s(-1) (oligo(dU)), and 42 nt s(-1) (ol

2 Sequences have been generated from 26 oligo(dT) primed directionally cloned libraries, of whic

3 Using 4 oligo(dT) 3' primers and 5 arbitrary decamers as 5' prim

4 described, employing multiple primers with 5'oligo(dT) sequences (MassTags) which serve to mass discr

5 f hepatitis C virus polymerase using poly(A)/oligo(dT(12)) that were or were not preannealed.

6 temperatures (T(m) values), however, poly(A)/oligo(dT(12-18)) is not expected to form stable duplexes

7 The conversion of D5 to a high affinity oligo(dT)-binding Fab occurred only in the presence of D

8 An oligo(dT)-primed target and three LCRs detect twice as m

9 s detected in reactions containing either an oligo(dT) primer alone, or in reactions supplemented wit

10 rst consists of an elongation reaction of an oligo(dT)(15) primer into poly(U).

11 to incorporate hundreds of nucleotides on an oligo(dT)-primed poly(dA) template with limiting amounts

12 recognizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, terminates 3' oligo

13 the sense primer, and a single base anchored oligo(dT) primer is used as an antisense primer in PCR,

14 the oligo(dT) primer with a set of anchored oligo(dT) primers for reverse transcription.

15 should be synthesized by use of the anchored oligo(dT) primers, rather than the oligo(dT) primers, to

16 omopolymer RNA template-primer, poly(A), and oligo(dT), the RT with altered Leu74Val mutation was les

17 etic oligonucleotides, such as oligo(dA) and oligo(dT), also stimulated the ATPase activity of the Mc

18 rform preannealing reactions for poly(A) and oligo(dT(12)), making it possible to characterize mechan

19 ity-determining region 3 (HCDR3) of the anti-oligo(dT) recombinant antibody fragment, DNA-1, contribu

20 ed with the structure of the complex between oligo(dT) and the D355A/E357A KF mutant.

21 The structure shows that DNA-1 binds oligo(dT) primarily by sandwiching thymine bases between

22 In this assay, a biotinylated oligo(dT(12)) primer was immobilized onto streptavidin-c

23 rted to blunt-ended, double-stranded cDNA by oligo(dT)-mediated reverse transcription followed by lin

24 ochondrial extract of rat cerebral cortex by oligo(dT)-cellulose affinity chromatography.

25 oli poly(A) polymerase I and purifying it by oligo(dT) chromatography.

26 the patterns of internal poly(A) priming by oligo(dT) primer.

27 ed or underrepresented after purification by oligo(dT).

28 e populations of mammalian mRNAs purified by oligo(dT) and 5' cap selection with oligonucleotide micr

29 poly(A)+ RNA from a starting RNA solution by oligo(dT)30 covalently linked to latex particles, buffer

30 ding of locked nucleic acid (LNA)-containing oligo(dT) probes to poly(A) tails to maximize RNA captur

31 capture (RIC) uses in vivo UV crosslinking, oligo(dT) capture, and proteomics to identify RNA-bindin

32 the three-subunit complex 4-fold on poly(dA)-oligo(dT) template-primer but had no effect on the activ

33 o[d(A-T)]), or straight and rigid [oligo(dA).oligo(dT)].

34 ant 125-kDa catalytic subunit using poly(dA).oligo(dT) as a template in the absence of proliferating

35 ta* showed a processivity of 2-3 on poly(dA).oligo(dT) compared with 5-10 for Poldelta.

36 -subunit enzyme retains activity on poly(dA)/oligo(dT) templates but is impaired in its ability to ex

37 x) is similar to that of gp5/trx on poly(dA)/oligo(dT).

38 tro processivity assays with excess poly(dA):oligo(dT) as a template, PF-8 stimulated the production

39 e by either anti-beta antibodies or poly(dA):oligo(dT).

40 With poly(dA):oligo(dT)50 as substrate, the exonucleolytic products fo

41 l gamma to extend a primer through a defined oligo(dT) tract.

42 oters but must rely on fortuitous downstream oligo(dT) tracts for terminator function.

43 constrain productivity and rely on expensive oligo(dT) ligands for target ss-mRNA poly(A) tail hybrid

44 group possesses relatively high affinity for oligo(dT) and may recognize single-stranded DNA ligands

45 rated that DNA-1 had a marked preference for oligo(dT) (100 nM dissociation constant) and required ol

46 te and the resultant fluorescent, hybridized oligo(dT) was tracked using high-speed imaging microscop

47 d that a large fraction of both intranuclear oligo(dT) (43%) and oligo(dA) (77%) moves rapidly with a

48 d a large fraction (45%) of the intranuclear oligo(dT), but not oligo(dA), diffusing at slower rates

49 ined further by a useful technique involving oligo(dT)-cellulose chromatography and antisense oligonu

50 Fluorescein-labeled oligo(dT) was introduced into HeLa cells stably expressi

51 g studies in which caged fluorescein-labeled oligo(dT) was used as hybridization probe, and the rate

52 late intermediate and is capable of ligating oligo(dT) strands annealed to poly(rA).

53 termini annealed to a template with a longer oligo(dT) tract are not efficiently extended by pol gamm

54 The amount of this slower-moving oligo(dT) was greatly reduced if the oligo(dT) was prehy

55 tensities were obtained with only 1% as much oligo(dT)-purified mRNA as total RNA, and hence in vitro

56 mRNAs could be detected on Northern blots of oligo(dT)-selected RNA from receptor-positive HeLa cells

57 to differential display with combinations of oligo(dT) and various arbitrary PCR primers.

58 Analyses of the protein composition of oligo(dT)-selected UV photocross-linked ER protein-RNA a

59 both subunits are able to bind an 18-mer of oligo(dT).

60 n was confirmed by screening the products of oligo(dT)-dependent cDNA synthesis.

61 poly(A) lengths or in the elution profile of oligo(dT)-bound targets.

62 We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without conside

63 ed the diffusion rate in aqueous solution of oligo(dT) hybridized to a large polyadenylated RNA (1.0

64 A subfraction of oligo(dT) (15%) moved over 10-fold more slowly, suggesti

65 efficiency of the thymidine oligonucleotide, oligo(dT)25, and providing a reliable surface for the am

66 a potential problem: amplifications based on oligo(dT) priming could be sensitive to RNA degradation;

67 ive stimulators than either oligo(dA)( )()or oligo(dT).

68 s mixed together with a mixture of 12 phased oligo(dT)25 antisense primers.

69 gainst either template RNA poly(A) or primer oligo(dT(12)) independently.

70 V-1 reverse transcriptase (RT) on a poly(rA).oligo(dT) template-primer (TP).

71 th poly(rC).oligo(dG), but not with poly(rA).oligo(dT), as the template/primer.

72 NTP were disrupted by adding excess poly(rA)/oligo(dT) or heparin just prior to electrophoresis.

73 In vivo UV-crosslinking of RNA to RBPs, oligo(dT) capture and mass spectrometry yielded 1,145 di

74 RNase H can even effectively replace oligo(dT)-based methods for standard RNA-seq.

75 (100 nM dissociation constant) and required oligo(dT) >5 nucleotides in length.

76 at 50 degrees C for 10 min by using the RNA-oligo(dT)30 hybrid on the latex particles as the templat

77 to the supernatant are separated by a second oligo(dT) selection.

78 using a number of oligonucleotide sequences: oligo(dT)(25) and a 30 bp oligonucleotide derived from b

79 ation within the pol III termination signal, oligo(dT).

80 so with precision and efficiency on a simple oligo(dT) tract, independent of other cis-elements or tr

81 asured diffusion rate for much of the slower oligo(dT) population approximated the diffusion rate in

82 These include- (a) use of standardized oligo(dT)-primed cDNA pools rather than total RNA as the

83 elements was replaced by two single-stranded oligo(dT)s is unwound by the UL9 protein-ICP8 complex.

84 These results demonstrate that oligo(dT) primed 5' capture scRNAseq can detect DENV RNA

85 ntroduction to cells, presumably because the oligo(dT) was then unavailable for subsequent hybridizat

86 regulated genes as could be detected by the oligo(dT)-primed target alone, in an experiment in which

87 oly(dT) sequences that are remnants from the oligo(dT)-primed reverse transcription of polyadenylated

88 e current system of gene identification: the oligo(dT) primer widely used for cDNA synthesis generate

89 -moving oligo(dT) was greatly reduced if the oligo(dT) was prehybridized in solution with (unlabeled)

90 n be effectively diminished by replacing the oligo(dT) primer with a set of anchored oligo(dT) primer

91 anchored oligo(dT) primers, rather than the oligo(dT) primers, to diminish the generation of truncat

92 B1/2 from glioma C6 cells do not bind to the oligo(dT)-based separation techniques efficiently, sugge

93 Laser spot photolysis then uncaged the oligo(dT) at a given intranuclear site and the resultant

94 the presence of poly(A) tracts by binding to oligo(dT) cellulose followed by hybridization with speci

95 nfirmed by immunoblotting fractions bound to oligo(dT)-cellulose and separated by rate sedimentation

96 rming ion pairs with dT5, Arg contributes to oligo(dT) recognition by helping to maintain the structu

97 that single mtSSB tetramers bind tightly to oligo(dT) single strands containing 32 to 48 residues.

98 f the H-chain of D5 Fab, restored binding to oligo(dT)15 to 60% of DNA-1 levels, whereas H-chain CDR

99 Using ITC, we find that DrSSB binding to oligo(dT)s with lengths close to the determined site siz

100 present its 3.5 angstrom structure bound to oligo(dT)9, (iii) provide data that implicate the HTH mo

101 was probed by photochemical cross-linking to oligo(dT)16.

102 both Hoechst-labeled chromosomes and uncaged oligo(dT) showed that, excluding nucleoli, the poly(A) R

103 e achieved by first synthesizing cDNAs using oligo(dT) coupled with magnetic beads which are then rec

104 changes that are difficult to measure using oligo(dT) priming for target synthesis.

105 s on the isolation of polyadenylated RNA via oligo(dT), it will not provide RNA-binding information o

106 ATPase activity of the Mcm467 complex, where oligo(dT) was more effective than oligo(dA).

107 HCDR3 residues of DNA-1 in interaction with oligo(dT) was elucidated.

108 migration were shown by RNase H mapping with oligo(dT) to be due to poly(A) shortening.

109 nked protein-RNA complexes are purified with oligo(dT) magnetic beads.

110 driving the first reverse transcription with oligo(dT) with a T7 RNA polymerase promoter (T7dT) on th

111 taining pcna-79 was unaffected on poly(dA) x oligo(dT) but was dramatically reduced on a natural temp