ライフサイエンスコーパス: oligonucleotide primer

1 enched once they are incorporated onto a DNA oligonucleotide primer.

2 tely 2.1 kb) by 'gene walking' using several oligonucleotide primers.

3 a polymerase chain reaction using degenerate oligonucleotide primers.

4 the array of PCR products starting from the oligonucleotide primers.

5 psis genome sequences to develop appropriate oligonucleotide primers.

6 e cDNA generated with oligo-dT and/or random oligonucleotide primers.

7 ersatile method to amplify specific DNA with oligonucleotide primers.

8 t any labeling requirements of the ddNTPs or oligonucleotide primers.

9 ng various combinations of randomly selected oligonucleotide primers.

10 merase chain reaction using random arbitrary oligonucleotide primers.

11 sing an Rh cE cDNA as template and mutagenic oligonucleotide primers.

12 y(A)+ mRNA was performed by using degenerate oligonucleotide primers.

13 nalysis greatly benefits from removal of the oligonucleotide primers (15- and 17-mers in this instanc

14 eoxyribo or ribonucleotide), embedded within oligonucleotide primers 29-30 nucleotides (nt), or great

15 T-PCR experiments using murine gene-specific oligonucleotide primers analyzed the scope and variety o

16 wo species by polymerase chain reaction with oligonucleotide primers anchored in the conserved 5S cod

17 BI Prism 7700 Sequence Detection System with oligonucleotide primers and a fluorescence-labeled probe

18 This assay used oligonucleotide primers and a TaqMan probe targeting the

19 erization of nucleotide precursors using two oligonucleotide primers and an amplification enzyme, as

20 PCR was performed with degenerate oligonucleotide primers and C. subterminale SB4 chromoso

21 Oligonucleotide primers and FAM- and TAMRA-labeled WN vi

22 he Primer3 utility exists for development of oligonucleotide primers and fills that role effectively.

23 Serotype- and group-specific oligonucleotide primers and fluorogenic probes were desi

24 esized HIV-1 (-)-strand strong-stop DNA from oligonucleotide primers and had minimal effect on RNase

25 We designed gene-specific oligonucleotide primers and probes for the measurement o

26 Oligonucleotide primers and probes were designed to dete

27 Oligonucleotide primers and probes were designed to targ

28 erase chain reaction assay and 5-LO-specific oligonucleotide primers and their mutated internal stand

29 nce of the CF-chitinase to design degenerate oligonucleotide primers and to amplify and sequence a PC

30 on into the standing start point of 5'-[32P]-oligonucleotide primer annealed with M13mp19 phage DNA b

31 f macaque with a consensus degenerate hybrid oligonucleotide primer approach.

32 Traditionally, oligonucleotide primers are 5'-(32)P labeled using T4 ki

33 Oligonucleotide primers are predicted automatically, usi

34 During the initiation of DNA replication, oligonucleotide primers are synthesized de novo by prima

35 templates by 1b-42 NS5B did not require the oligonucleotide primer at high concentrations (>/=50 mic

36 he convenient approaches of either designing oligonucleotide primers at the splice junction or differ

37 eir activity-dependent modification of a DNA oligonucleotide primer attached to the same phage partic

38 A degenerate oligonucleotide primer based upon internal amino acid se

39 Degenerate oligonucleotide primers based on conserved amino acid re

40 actions (PCRs) performed with mixed sequence oligonucleotide primers based on conserved regions.

41 Oligonucleotide primers based on exon-flanking sequences

42 Using PCR and degenerate oligonucleotide primers based on internal peptide sequen

43 he polymerase chain reaction with degenerate oligonucleotide primers based on sequences common to thr

44 Two pairs of oligonucleotide primers based on the 16S rDNA gene were

45 Using oligonucleotide primers based on the deduced DNA sequenc

46 Oligonucleotide primers based on the human heart monocar

47 Oligonucleotide primers based on the murine sequence wer

48 Oligonucleotide primers based on the partial amino acid

49 Nondegenerate oligonucleotide primers based on the porcine cDNA were s

50 Using oligonucleotide primers based on the reported human CLN3

51 With a set of oligonucleotide primers based on the same primer binding

52 creen a mouse pcDNA3 expression library with oligonucleotide primers based on the translated human le

53 Degenerate oligonucleotide primers based upon this information were

54 Degenerate oligonucleotide primers, based on a partial internal ami

55 Degenerate oligonucleotide primers, based on conserved sequences in

56 rases, which are able to extend the attached oligonucleotide primer by incorporating ribonucleoside t

57 Nested PCR with patient IgH allele-specific oligonucleotide primers can detect 1 tumor cell in 10(4)

58 Using a panel of 19 V beta-specific oligonucleotide primers, changes in the level of TCR V b

59 s for the detection of parasite DNA by using oligonucleotide primers COC-1 and COC-2 for PCR amplific

60 eospora spp. and other apicomplexa coccidia, oligonucleotide primers COC-1 and COC-2 were used for PC

61 This COnsensus-DEgenerate Hybrid Oligonucleotide Primer (CODEHOP) strategy has been imple

62 vel HHVs, using consensus-degenerated hybrid oligonucleotide primers (CODEHOP) PCR: no sequence indic

63 lification using COnsensus DEgenerate Hybrid Oligonucleotide Primers (CODEHOPs) has proven to be high

64 quences based on consensus-degenerate hybrid oligonucleotide primers (CODEHOPs).

65 rally occurring microbial populations, using oligonucleotide primers complementary to highly conserve

66 Oligonucleotide primers complementary to the flanking un

67 ntly elongating completely non-telomeric DNA oligonucleotide primers, consisting of natural telomere-

68 s synthesized by a method that uses two long oligonucleotide primers containing primer binding sites

69 CAPS markers for this study, eight pairs of oligonucleotide primers corresponding to five previously

70 1 gene was isolated using PCR and degenerate oligonucleotide primers corresponding to highly conserve

71 Oligonucleotide primers corresponding to Sp3 messenger R

72 mplification of Mortierella alpina cDNA with oligonucleotide primers corresponding to the conserved r

73 reactive 5-iododeoxypyrimidines into the DNA oligonucleotide primer d(T4G4T4G4T4G2) and, upon irradia

74 ymerase chain reaction with isoform-specific oligonucleotide primers demonstrated the presence of the

75 a deduced 36-kDa protein by using degenerate oligonucleotide primers derived from a 30-residue peptid

76 olymerase chain reaction amplification using oligonucleotide primers derived from conserved regions o

77 by polymerase chain reaction with degenerate oligonucleotide primers derived from conserved segments

78 Using oligonucleotide primers derived from rat CD13 cDNA, a mo

79 y polymerase chain reaction using degenerate oligonucleotide primers derived from regions of sequence

80 Oligonucleotide primers derived from sequences shared by

81 T-PCR with mRNA from wheat crown tissue, the oligonucleotide primers derived from the barley cognate

82 The consensus-degenerate hybrid oligonucleotide primer design and seminested or nested P

83 PROBER is an oligonucleotide primer design software application that

84 A single oligonucleotide primer, designated iRepl, was designed t

85 eaction-based approach relying on degenerate oligonucleotide primers designed according to the amino

86 Oligonucleotide primers designed according to the Rx1 ps

87 PCR using oligonucleotide primers designed for a conserved protein

88 In this study, oligonucleotide primers designed for conserved sequences

89 Oligonucleotide primers designed for conserved sequences

90 p samples were analyzed by nested PCR, using oligonucleotide primers designed for the gene encoding t

91 By using oligonucleotide primers designed from peptide sequence i

92 encoding the proteinase was amplified using oligonucleotide primers designed from the 45 kDa N-termi

93 ed with the inclusion in the PCR reaction of oligonucleotide primers designed to amplify a 3016-bp fr

94 eaction (PCR) in the presence of a series of oligonucleotide primers designed to amplify all 7 exons

95 in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTA

96 Degenerate oligonucleotide primers designed to the putative calmodu

97 aining a polymorphic locus is incubated with oligonucleotide primers (designed to hybridize to the DN

98 y in the mutant hpt gene product, degenerate oligonucleotide primers, designed according to the N- an

99 ples with IsoQuick was amplified by PCR with oligonucleotide primers directed to the DNA polymerase g

100 n RdRp and sensitive to the concentration of oligonucleotide primer directing RNA synthesis.

101 ducing genome complexity by using degenerate oligonucleotide primer (DOP)-PCR and applied this strate

102 e 'random' sequences amplified by degenerate oligonucleotide primer (DOP)-PCR can be precisely mapped

103 Initially, multiple degenerate oligonucleotide primers (DOP) and probes were designed f

104 amage tolerance, PrimPol synthesises de novo oligonucleotide primers downstream of polymerase-stallin

105 Degenerate oligonucleotide primers encoding two of the polypeptide

106 ry to accurately measure the masses of short oligonucleotide primers extended by a single dideoxynucl

107 subjected to polymerase chain reaction with oligonucleotide primers for 12 different viruses (cytome

108 ed a method using a single set of degenerate oligonucleotide primers for amplification of the conserv

109 We have designed a series of intronic oligonucleotide primers for amplifying the entire coding

110 Oligonucleotide primers for bile salt-stimulated cholest

111 flower cDNA library by PCR using degenerate oligonucleotide primers for conserved domains of protein

112 In contrast, oligonucleotide primers for hormone-sensitive lipase yie

113 rase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the reaction.

114 N-terminal sequence enabled construction of oligonucleotide primers for PCR and RACE-derived cDNAs f

115 of C57BL/6 mice through an optimized set of oligonucleotide primers for qRT-PCR assays and cloned cD

116 Using degenerate oligonucleotide primers for the NBS region of N and RPS2

117 Oligonucleotide primers for this sequence were used to a

118 loyed 3' RACE PCR, using a highly degenerate oligonucleotide primer, for the facile isolation of a C2

119 Oligonucleotide primers from sequences that flank 224 of

120 ucleic acids by amplification with arbitrary oligonucleotide primers has become popular because it ca

121 se has been observed to add repeats to a DNA oligonucleotide primer in a processive manner, leading t

122 s indicate that telomerase may interact with oligonucleotide primers in a bipartite manner, with the

123 By utilizing degenerate oligonucleotide primers in a reverse transcriptase-coupl

124 d bacterial 16S ribosomal RNA using specific oligonucleotide primers in polymerase chain reaction (PC

125 on of the genetic material by using specific oligonucleotide primers in polymerase chain reaction.

126 Telomerase can extend oligonucleotide primers in vitro in a processive fashion

127 of rat liver poly(A)(+) RNA using 5'-derived oligonucleotide primers indicated that the 5' end sequen

128 Degenerate oligonucleotide primers inferred from peptide sequence d

129 An oligonucleotide primer is annealed to the target DNA imm

130 " In immunoRCA, an oligonucleotide primer is covalently attached to an Ab;

131 Primer extension analysis using two 30-mer oligonucleotide primers known to be contained within the

132 This method utilizes oligonucleotide primers labeled with one of three fluore

133 However, the DNA oligonucleotide primers needed for ChIP-qPCR are more ch

134 y conventional synthesis at the 5'-end of an oligonucleotide primer of any sequence.

135 , in which we modify the immobilized lawn of oligonucleotide primers of a next-generation DNA sequenc

136 hain reaction of genomic DNA with degenerate oligonucleotide primers, one based on the N-terminal ami

137 -OddCMP from the 3'-end of a single-stranded oligonucleotide primer or a primer annealed with complem

138 es not copy these RNAs unless a suitable DNA oligonucleotide primer or DNA target site is provided.

139 rmed using template mRNA from T560 cells and oligonucleotide primer pairs designed from the published

140 ay consists of six molecular beacons and two oligonucleotide primer pairs, allowing for detection and

141 ypanosoma cruzi proteins by using degenerate oligonucleotide primers prepared against conserved domai

142 ed at the 5' end of a 5' fluorophore-labeled oligonucleotide primer, quenching the primer's fluoresce

143 of a tung seed cDNA library using degenerate oligonucleotide primers resulted in identification of tw

144 ens RNA and casein kinase I isoform-specific oligonucleotide primers resulted in the amplification of

145 Using an oligonucleotide primer set specific for MN-complimentary

146 An oligonucleotide primer specific for a conserved amino ac

147 of the organisms was confirmed by PCR using oligonucleotide primers specific for E. equi and the HGE

148 ial 16S rDNA was amplified using 2 synthetic oligonucleotide primers specific for eubacteria.

149 liver and human cells to perform RT-PCR with oligonucleotide primers specific for nucleus-encoded tRN

150 PCR) using RNA isolated from WIF-B cells and oligonucleotide primers specific for rat ntcp or human N

151 eudogene was identified by inverse PCR using oligonucleotide primers specific for the 5' region of th

152 A set of universal oligonucleotide primers specific for the conserved regio

153 Using RT-PCR with pairs of sense-antisense oligonucleotide primers specific for the various regions

154 and reverse transcriptase PCR (RT-PCR) with oligonucleotide primers specific to the major subunit, a

155 96 and PCR amplification using dual-priming oligonucleotide primers specific to the V1-V3 region of

156 Oligonucleotide primers targeting prp and cps were combi

157 on of a single nucleotide into a short 25/45 oligonucleotide primer-template by pol beta was used to

158 An oligonucleotide primer-template was designed with an 18-

159 of DNA polymerase I and a series of defined oligonucleotide primer/templates.

160 omparable to radiolabeling is achieved using oligonucleotide primers that are 5'-end labeled with inf

161 Specific oligonucleotide primers that differentiate 1302A from RJ

162 In this method, oligonucleotide primers that have different molecular we

163 om these three groups of mutants by PCR with oligonucleotide primers that hybridize to flanking regio

164 ed the binding properties of single-stranded oligonucleotide primers that serve as telomerase substra

165 cDNA and by RT-PCR analysis with a series of oligonucleotide primers that span the entire cDNA for ap

166 Using oligonucleotide primers that specifically amplify human

167 of different Abs, each labeled with a unique oligonucleotide primer, that can be discriminated by a c

168 olymerase chain reaction with two degenerate oligonucleotide primers, the design of which was based o

169 We show here, using only 4 nested oligonucleotide primers, the successful amplification an

170 plished by hybridizing a short complementary oligonucleotide primer to the target and extending the r

171 GSTs are shown to be long enough for use as oligonucleotide primers to amplify adjacent segments of

172 olymerase chain reaction (PCR) cloning using oligonucleotide primers to DNA sequences conserved withi

173 d a PCR approach employing highly degenerate oligonucleotide primers to isolate several Arabidopsis g

174 rformed using previously reported degenerate oligonucleotide primers to the ligand binding domain (LB

175 Degenerate oligonucleotide primers to the N-terminal sequence of th

176 Oligonucleotide primers to the S2 segment of CTF (Florio

177 by PCR of human brain cDNA using degenerate oligonucleotide primers to transmembrane (TM) domains 3

178 ion, in conjunction with a set of degenerate oligonucleotide primers, to identify a subset of HOX gen

179 SOP3v2-generated oligonucleotide primer trios enable analysis of single n

180 polymerase catalytic subunit, interacts with oligonucleotide primers via two uridylate recognition si

181 The PLC-gamma 1 specific antisense oligonucleotide primer was able to inhibit cell prolifer

182 ucleotide at the 3'-end of each CFET-labeled oligonucleotide primer was complementary to a particular

183 A pair of oligonucleotide primers was designed based on the potato

184 tion-based strategy combined with degenerate oligonucleotide primers was employed.

185 A library of CFET-labeled oligonucleotide primers was mixed with biotin-ddNTPs, DN

186 e chain reaction (RT-PCR) with gene-specific oligonucleotide primers was performed to characterize th

187 Using rat sex-determining region-Y-specific oligonucleotide primers, we determined the donor DNA con

188 Using exon-4-specific oligonucleotide primers, we have amplified, cloned, and

189 nucleotides (dNTPs) at the 3'-OH group of an oligonucleotide primer; we term this methodology surface

190 In a complementary strategy, degenerate oligonucleotide primers were designed against highly con

191 Degenerate oligonucleotide primers were designed based on sequence

192 om the purified 175-kDa HARE, and degenerate oligonucleotide primers were designed for reverse transc

193 Consensus-degenerate hybrid oligonucleotide primers were designed from an internal V

194 In this study, oligonucleotide primers were designed from regions of th

195 Single-stranded oligonucleotide primers were designed to allow loop form

196 Oligonucleotide primers were designed to amplify each ex

197 Oligonucleotide primers were designed to amplify specifi

198 5' and 3' rapid amplification of cDNA ends, oligonucleotide primers were designed to amplify the ent

199 acid sequences derived from the 20-kDa band, oligonucleotide primers were designed, two of which prod

200 Oligonucleotide primers were developed for nested PCR ba

201 32P-Radiolabeled RNA oligonucleotide primers were incubated with yPAP in the

202 For each repeat, flanking oligonucleotide primers were synthesized and the polymer

203 Degenerate oligonucleotide primers were used in PCR to amplify a re

204 s obtained, and inosine-containing redundant oligonucleotide primers were used to amplify an 815-base

205 Degenerate oligonucleotide primers were used to amplify by polymera

206 Degenerate oligonucleotide primers were used to amplify conserved p

207 Degenerate oligonucleotide primers were used to obtain a polymerase

208 ide sequences were used to design degenerate oligonucleotide primers which were then used as a first

209 peptides allowed construction of degenerate oligonucleotide primers, which amplified a 551-base pair

210 es the development of appropriately targeted oligonucleotide primers, which necessitates the identifi

211 Three unique SBE oligonucleotide primers, which probe for mutations of cl

212 potato (Solanum tuberosum) cv. Desiree using oligonucleotide primers with sequences which are highly