ライフサイエンスコーパス: oligoribonucleotide

1 are unable to either synthesize or extend an oligoribonucleotide.

2 and 3' flanking RNAs yields the full-length oligoribonucleotide.

3 cleotide substitutions at the 3' pole of the oligoribonucleotide.

4 her synthesize primers de novo nor extend an oligoribonucleotide.

5 ablation of the 5'-most cleavage site on the oligoribonucleotide.

6 ngly in vitro to synthetic poly-U and poly-A oligoribonucleotides.

7 with the formation of several short abortive oligoribonucleotides.

8 roup at specified positions within synthetic oligoribonucleotides.

9 oligonucleotides were bound to unstructured oligoribonucleotides.

10 binds independently to S/R and thiostrepton oligoribonucleotides.

11 times (2 min) for the automated synthesis of oligoribonucleotides.

12 usion of guanosine monophosphate or specific oligoribonucleotides.

13 ical ribonucleotides and their assembly into oligoribonucleotides.

14 on of purine-2',3'-cyclophosphate-terminated oligoribonucleotides.

15 ligoribonuclease, causes the accumulation of oligoribonucleotides 2 to approximately 4 nt in length,

16 Essentially, 2'- O -methyl oligoribonucleotides (2'OMeRNA) were delivered to the nu

17 a 2-O-methylated phosphorothioated antisense oligoribonucleotide (2OMeAO) designed to promote skippin

18 cific 2'-O-methyl phosphorothioate antisense oligoribonucleotide (2OMeAO).

19 Here we show that the 2'-hydroxyl group of oligoribonucleotide-3'-phosphates can be chemoselectivel

20 An oligoribonucleotide (a 27-mer) that mimics the sarcin/ri

21 However, NMR spectroscopy of an oligoribonucleotide, a 29-mer that mimics the sarcin/ric

22 ransfection of enterocytes with an antisense oligoribonucleotide against miR-122a blocked the TNF-alp

23 Indeed, Hfq bound to the oligoribonucleotides (AGG)(8), (AGC)(8), and the shorter

24 chimeric primases catalyze the synthesis of oligoribonucleotides albeit at a reduced rate and DNA de

25 EF-G also binds to an oligoribonucleotide (an 84-mer) that has the thiostrepto

26 A 17 base oligoribonucleotide analog of the T-arm was equivalent t

27 labeling either the 5' or the 3' end of the oligoribonucleotide and by examining the reaction produc

28 The number of nitrogen atoms in the oligoribonucleotide and fragment ions can readily be det

29 phosphodiester/phosphorothioate 2'-O-methyl-oligoribonucleotides and 6-9- and almost 200-fold more e

30 P6 primase incorporates GTP at the 5'-end of oligoribonucleotides and CMP at the second position.

31 ion sufficient for site-specific cleavage of oligoribonucleotides and complex RNAs.

32 onal stabilizing interactions between capped oligoribonucleotides and eIF4E, which do not occur with

33 nity selection with biotinylated 2'-O-methyl oligoribonucleotides and glycerol gradient fractionation

34 n have triggered a high demand for synthetic oligoribonucleotides and have motivated the development

35 n (ATRP) initiator into both short synthetic oligoribonucleotides and natural biomass RNA extracted f

36 for the identification and quantification of oligoribonucleotides and post-transcriptional modificati

37 of 2'-SCF3 pyrimidine nucleoside containing oligoribonucleotides and the comprehensive investigation

38 livery of RNAs (siRNAs, miRNA or other short oligoribonucleotides) and small-molecule drugs.

39 of kissing and duplex dimer forms of related oligoribonucleotides, and nucleotides C1-G4 and C27-G30

40 quence mapping was performed using automated oligoribonucleotide annotation and identifications based

41 the cells with 2'-O-methyl phosphorothioate oligoribonucleotides antisense toward the aberrant donor

42 our in vitro results, we postulate that the oligoribonucleotides are able to prime synthesis of wild

43 2'-O-Ethylene glycol substituted oligoribonucleotides are second-generation antisense inh

44 ological properties of these novel synthetic oligoribonucleotides as antagonists of TLRs 7, 8 and 9 i

45 lthough the latter are effective in cleaving oligoribonucleotides, as predicted.

46 primase fragment catalyzes the synthesis of oligoribonucleotides at rates similar to those catalyzed

47 oate oligonucleotides containing 2'-O-methyl oligoribonucleotides at the 3' and 5' termini.

48 cture reveals that the single-stranded hepta-oligoribonucleotide binds in a circular conformation aro

49 y the primase fragment at the 5'-ends of the oligoribonucleotides but not at the 3'-ends.

50 termined the structure of the 19-residue SL2 oligoribonucleotide by heteronuclear NMR methods.

51 mechanism involves the production of 4-8 nt oligoribonucleotides by abortive synthesis by the viral

52 eIF4E (eIF4E(P)) with cap analogs and capped oligoribonucleotides by stopped-flow kinetics.

53 peptides slow the annealing of complementary oligoribonucleotides by up to several thousand-fold; how

54 hat contain synthetic genes coding for small oligoribonucleotides called external guide sequences (EG

55 Because the cleavage of the synthetic oligoribonucleotide can be used to monitor the steady-st

56 We also showed that the modified oligoribonucleotide can switch the reverse transcription

57 gment ions in both the unlabeled and labeled oligoribonucleotides can be used to gain further confide

58 n of prepro-nociceptin are identified by an 'oligoribonucleotide-capping' method, but the major one i

59 nd potential improvements in the efficacy of oligoribonucleotides, chemical modification may also pro

60 DNA synthesis was initiated from (i) an oligoribonucleotide complementary to the primer-binding

61 re it is shown that binding of a 2'-O-methyl-oligoribonucleotide complementary to U5 small nuclear RN

62 Antisense 2'-O-methyl oligoribonucleotides complementary to selected SF2/ASF b

63 d the crystal structure of an Sa Hfq-adenine oligoribonucleotide complex.

64 between EF-G and a sarcin/ricin domain (SRD) oligoribonucleotide containing 5-iodouridine.

65 oaches to a dinucleotide, trinucleotide, and oligoribonucleotide containing a photocaged 2'-amino-5'-

66 G, but it cannot catalyze the cleavage of an oligoribonucleotide containing only cytosines.

67 A biotinylated oligoribonucleotide containing the direct repeat was use

68 Moreover, chimeric oligoribonucleotides containing 2'-deoxy- or arabinonucl

69 tability is comparable to that observed with oligoribonucleotides containing 2'-O-methylated residues

70 Oligoribonucleotides containing 3'-S-phosphorothiolate l

71 To facilitate the synthesis of oligoribonucleotides containing 4-thiouridine, we have d

72 We envisioned that oligoribonucleotides containing a 2'-amino-5'-S-phosphor

73 hosphoramidites for solid-phase synthesis of oligoribonucleotides containing a 2'-O-photocaged 5'-S-p

74 Oligoribonucleotides containing a 5'-phosphorothiolate l

75 Oligoribonucleotides containing a photocaged 2'-amino-5'

76 cribes a general method for the synthesis of oligoribonucleotides containing a site-specific nonbridg

77 A series of oligoribonucleotides containing different 3 x 3 internal

78 endent RNA polymerase that synthesizes short oligoribonucleotides containing each of the four canonic

79 cal melting experiments, are reported for 28 oligoribonucleotides containing frequently occurring sin

80 Optical melting experiments on oligoribonucleotides containing internal loops of three

81 By using synthetic oligoribonucleotides containing repeats of identical tar

82 ting studies are reported for a series of 14 oligoribonucleotides containing single I x U pairs adjac

83 o determine the thermodynamic parameters for oligoribonucleotides containing small asymmetric interna

84 105-115 of the 5'-UTR (U5) readily binds to oligoribonucleotides containing the gag start codon (AUG

85 phosphoramidite and synthesized a series of oligoribonucleotides containing this photoswitchable res

86 hotoisomerization in both ribonucleoside and oligoribonucleotide contexts and studied the overall imp

87 Using a 17 residue oligoribonucleotide corresponding to the anticodon arm o

88 A 17-base oligoribonucleotide corresponding to the anticodon stem-

89 rected mutants using DMAPP and a 17-base RNA oligoribonucleotide corresponding to the stem-loop regio

90 ynthetic pre-miRNAs and even single-stranded oligoribonucleotides corresponding to miRNA loops, we re

91 ligodeoxyribonucleotide (MPO) and its target oligoribonucleotide, d(T(MP)CC(MP)-TT(MP)AG(MP)CT(MP)CC(

92 Extracellularly delivered ssRNA40, an oligoribonucleotide derived from HIV and an established

93 preparation of branched and branched cyclic oligoribonucleotides derived from adenosine.

94 tic, template-directed ligation reactions of oligoribonucleotides display high selectivity for the fo

95 While other cellular RNases process oligoribonucleotides down to diribonucleotide entities,

96 e derived from optical melting studies of 90 oligoribonucleotide duplexes containing only Watson-Cric

97 Oligoribonucleotide duplexes containing small internal l

98 Furthermore, oligoribonucleotide duplexes with parallel strand orient

99 chemical stability of these highly activated oligoribonucleotides during synthesis and long-term stor

100 gonucleotides were prehybridized with 17-mer oligoribonucleotides, extracts prepared from T24 cells,

101 DNA primases provide oligoribonucleotides for DNA polymerase to initiate lagg

102 The primase catalyzes the synthesis of oligoribonucleotides for the initiation of lagging stran

103 TTP, and catalyze the synthesis of short RNA oligoribonucleotides for use as primers by T7 DNA polyme

104 nder them superior to corresponding 2'-deoxy oligoribonucleotides for use in assays that detect RNA t

105 yme, Dcs1, catalyses cleavage of 5'end m(7)G-oligoribonucleotide fragments generated by 3'-->5' exonu

106 Reduction in Ha-Ras RNA was dependent on the oligoribonucleotide gap size with the minimum gap size b

107 osol, and nuclei resulted in cleavage in the oligoribonucleotide gap.

108 thoxy 5' and 3' "wings" on either side of an oligoribonucleotide gap.

109 Synthesis of partially 2'/3'-O-acetylated oligoribonucleotides has been accomplished by using a 2'

110 petitions in Xenopus oocytes with 2'O-methyl oligoribonucleotides have confirmed this region as a fun

111 Incorporation of 4'-thiocytidines into oligoribonucleotides improved the thermal stability of t

112 A random coil 17-base oligoribonucleotide in which the loop sequence of E. col

113 ective than the phosphorothioate 2'-O-methyl-oligoribonucleotides in free uptake from the culture med

114 T7 gene 4 protein catalyzes the synthesis of oligoribonucleotides in the presence of ATP, CTP, Mg(2+)

115 purified recombinant D5 protein synthesized oligoribonucleotides in vitro.

116 sis of the effect of alpha-sacrin on variant oligoribonucleotides in which additional bases were inse

117 tes the rapid and accurate quantification of oligoribonucleotides, including cyclic phosphate interme

118 Here we provide a study of oligoribonucleotide interaction kinetics and show that i

119 Our studies show that the 5' GG of the bound oligoribonucleotide interacts extensively with highly co

120 s a demonstration, invasion of a 2'-O-methyl oligoribonucleotide into an RNA hairpin model (HIV-1 TAR

121 trate that injection of 2'O-methyl antisense oligoribonucleotides into early Drosophila embryos leads

122 that can be used to address anti-replicative oligoribonucleotides into human mitochondria and thus im

123 dicate that the 5'-most cleavage site on the oligoribonucleotide is positioned 7 bp from the first 3'

124 ound to ribosomes and the analog of G2655 in oligoribonucleotides is critical for recognition by the

125 thesis and its subsequent incorporation into oligoribonucleotides is described.

126 ase are crucial for the stabilization of the oligoribonucleotide, its transfer to the polymerase, and

127 nt of an efficient chemical strategy to make oligoribonucleotide-ligand conjugates using the copper-c

128 f pyrophosphate-activated, template-directed oligoribonucleotide ligation has been investigated.

129 An increase in the stability of the mutant oligoribonucleotides may be the basis of the impairment

130 eterohexamers synthesize a reduced amount of oligoribonucleotides, mediated predominately by the 63-k

131 thin the intron substrates (guanosine and an oligoribonucleotide mimic of the 5'-exon) that coordinat

132 Short synthetic oligoribonucleotide mimics of the RNA-1 TABS and the RNA

133 rization of RNA mimetics, uniformly modified oligoribonucleotide N3'-->P5' phosphoramidates containin

134 The properties of the oligoribonucleotide N3'-->P5' phosphoramidates indicate

135 thesis and properties of novel RNA mimetics, oligoribonucleotide N3'-->P5' phosphoramidates, are desc

136 ce for binding to poly(A) RNA relative to an oligoribonucleotide of the same length and a random sequ

137 apid purification and structural analysis of oligoribonucleotides of 19 and 20 nt is applied to RNA h

138 Titration experiments with oligoribonucleotides of varying lengths and compositions

139 onucleotide analogues, namely, a 2'-O-methyl oligoribonucleotide (OMe), a chimeric oligonucleotide co

140 imase of phage T7 catalyzes the synthesis of oligoribonucleotides on single-stranded DNA templates.

141 tivity, catalysing the synthesis of unprimed oligoribonucleotides on single-stranded DNA templates.

142 otides, but T7 primase can synthesize longer oligoribonucleotides on templates containing long stretc

143 Recombinant Mjpri is able to synthesise oligoribonucleotides on various pyrimidine single-strand

144 identifies a novel class of single-stranded oligoribonucleotides (ORN) containing unmethylated CpG m

145 art from foreign and host RNA, synthetic RNA oligoribonucleotides (ORN) or small molecules of the imi

146 oducing precise 2' sugar-modified bases into oligoribonucleotides (ORNs) containing known TLR7 and TL

147 bit TS expression, antisense 2'-O-methyl RNA oligoribonucleotides (ORNs) were designed to directly ta

148 by short, nuclease-resistant, non-extendable oligoribonucleotides (ORNs).

149 R-17-92 knockdown with antisense 2'-O-methyl oligoribonucleotides partly restored Tsp1 and CTGF expre

150 Several mixed base 9-13mer oligoribonucleotide phosphoramidates were synthesized wi

151 ge T7 primase catalyzes the synthesis of the oligoribonucleotides pppACC(C/A) and pppACAC from the si

152 igated whether lexaptepid, an antihepcidin l-oligoribonucleotide, prevents the decrease in serum iron

153 mediated extension of abortively synthesized oligoribonucleotide primers complementary to the 3' end

154 Synthesis of oligoribonucleotide primers for lagging-strand DNA synth

155 te-dependent RNA polymerases that synthesize oligoribonucleotide primers that can be extended by DNA

156 d (ss) DNAs as templates to synthesize short oligoribonucleotide primers that initiate lagging strand

157 nt RNA polymerases have been reported to use oligoribonucleotide primers to initiate nucleic acid syn

158 n of rRNA whereas the same 17 base 2'- deoxy oligoribonucleotide probe did not.

159 A 12 base 2'-O-methyl oligoribonucleotide probe had the same Tm as a 19 base 2

160 A 17 base 2'-O-methyl oligoribonucleotide probe was able to bind a double-stra

161 probe had the same Tm as a 19 base 2'-deoxy oligoribonucleotide probe when bound to a matched RNA ta

162 much larger decrease in Tm than the 2'-deoxy oligoribonucleotide probe when bound to an RNA target co

163 ures (Tm values) than corresponding 2'-deoxy oligoribonucleotide probes at all lengths tested (8-26 b

164 In contrast to RNA targets, 2'-O-methyl oligoribonucleotide probes bound more slowly and with th

165 2'-O-Methyl oligoribonucleotide probes bound to RNA targets faster a

166 en bound to RNA targets, shorter 2'-O-methyl oligoribonucleotide probes can be used in assays in plac

167 y enhanced Tm when bound to RNA, 2'-O-methyl oligoribonucleotide probes can efficiently bind to doubl

168 ed various kinetic and melting properties of oligoribonucleotide probes containing 2'-O-methylnucleot

169 ences in Tm between 2'-O-methyl and 2'-deoxy oligoribonucleotide probes observed at lengths of 16 bas

170 ets and increased specificity of 2'-O-methyl oligoribonucleotide probes render them superior to corre

171 e used in assays in place of longer 2'-deoxy oligoribonucleotide probes, resulting in enhanced discri

172 Tm to DNA targets as corresponding 2'-deoxy oligoribonucleotide probes.

173 a single-step deprotection of the resulting oligoribonucleotide product using 1,2-diamines under anh

174 fraction of the resulting products is small oligoribonucleotides rather than the mononucleotides gen

175 neral semisynthetic strategy to obtain these oligoribonucleotides reliably and relatively efficiently

176 l proteins (nsPs) and a (32)P-labeled 24-mer oligoribonucleotide representing the minimal sequence wi

177 tro and in vivo, and inclusion of competitor oligoribonucleotides representing the USEs specifically

178 DNA primases catalyze the synthesis of oligoribonucleotides required for the initiation of lagg

179 DNA primases catalyze the synthesis of the oligoribonucleotides required for the initiation of lagg

180 introducing a synthetic 2'-O-methyl tRF-1001 oligoribonucleotide resistant to the siRNA.

181 udies with RNA homopolymers and a variety of oligoribonucleotides revealed that RNase T displays an u

182 and have a modified oligodeoxynucleotide or oligoribonucleotide segment located in the central porti

183 The corresponding oligoribonucleotide sequences (5' rGCGAAUUCGC)2, (5' rGC

184 These methods produce branched oligoribonucleotide sequences of arbitrary length, base

185 This means that phosphorothioate-containing oligoribonucleotides should also be useful for mapping p

186 The rate constant for association of the oligoribonucleotide substrate (S) increased 12-fold from

187 amino-5'-S-phosphorothiolate linkage into an oligoribonucleotide substrate for the hepatitis delta vi

188 No base specificity was observed with an oligoribonucleotide substrate.

189 e recognition of AREs by AUF1 in vitro using oligoribonucleotide substrates.

190 y, indicates amino acids likely critical for oligoribonucleotide synthesis as well as a putative Cys(

191 However, oligoribonucleotide synthesis by SP6 primase is not stim

192 T7 DNA primase to catalyze template-directed oligoribonucleotide synthesis is eliminated by substitut

193 keeping with this property, Mcm10p supported oligoribonucleotide synthesis of short RNA primers (pref

194 itate DNA binding, leading to more efficient oligoribonucleotide synthesis on short templates.

195 to the 5'-end have no effect on the rate of oligoribonucleotide synthesis or the affinity of the pri

196 ing d2APy in place of the cryptic dC support oligoribonucleotide synthesis whereas those containing 3

197 oxyuridine (dU) substitutions for dT support oligoribonucleotide synthesis whereas those containing 5

198 a primase recognition site does not inhibit oligoribonucleotide synthesis, suggesting that the prima

199 2'-deoxyinosine (dI) in place of dG support oligoribonucleotide synthesis.

200 CTP are both required for the initiation of oligoribonucleotide synthesis.

201 are required for tight DNA binding and rapid oligoribonucleotide synthesis.

202 , in part, responsible for template-directed oligoribonucleotide synthesis.

203 conformation leads to loss of DNA-dependent oligoribonucleotide synthesis.

204 domain is responsible for template-directed oligoribonucleotide synthesis.

205 The T7 DNA polymerase can use oligoribonucleotides synthesized by SP6 primase as prime

206 he lagging-strand DNA polymerase requires an oligoribonucleotide, synthesized by DNA primase, to init

207 e incorporated into an antisense 2'-O-methyl oligoribonucleotide targeted to the 3' region of the PKC

208 r, treatment of the cells with a 2'-O-methyl-oligoribonucleotide targeted to the aberrant splice site

209 ngle-stranded antisense 2'-O-methyl (2'-OMe) oligoribonucleotides targeting microRNA (miRNA).

210 ti-hepcidin compound NOX-H94, a structured L-oligoribonucleotide that binds human hepcidin with high

211 nds YS11 with a Kd value of 230 nM; a second oligoribonucleotide that contains only the kink-turn mot

212 In the presence of a specific competitor oligoribonucleotide that inhibits MnSOD RNA protein-bind

213 An oligoribonucleotide that mimics helix 11, a phylogenetic

214 317 and C4331, and to U4316 and C4332, in an oligoribonucleotide that mimics the sarcin/ricin domain

215 The oligoribonucleotides that accumulate are 2-5 residues in

216 ase L can catalyze the cleavage of synthetic oligoribonucleotides that contain dyad sequences of the

217 ve studied its interactions with a series of oligoribonucleotides that contain one or more of the Psi

218 n processing long, structured RNA into short oligoribonucleotides that engage TLR7 or TLR8.

219 A series of oligoribonucleotides that form purine-rich internal loop

220 mal protein, in a filter retention assay, to oligoribonucleotides that reproduce regions of 18 S rRNA

221 t a replication fork DNA primase synthesizes oligoribonucleotides that serve as primers for the laggi

222 Deletion from oligoribonucleotides, that reproduce the sarcin/ricin do

223 Although it is not copied into the oligoribonucleotides, the cytosine at the 3'-position is

224 8 and G2663 decreased binding of EF-G to SRD oligoribonucleotides; the same mutations in 23 S rRNA de

225 teractions that occur during delivery of the oligoribonucleotide to DNA polymerase, we have used four

226 sense sequence hybridized to a complementary oligoribonucleotide to evaluate both the binding affinit

227 nvolves use of RNA ligase to link a specific oligoribonucleotide to the 5' ends of cellular RNAs, fol

228 re conducted with chemically synthesized RNA oligoribonucleotides to determine the essential elements

229 DNA primases catalyze the synthesis of oligoribonucleotides to initiate lagging strand DNA synt

230 on that does not require base pairing of the oligoribonucleotides to the mutant, positive-strand RNA

231 clear magnetic resonance of synthetic G-rich oligoribonucleotide tracts derived from this region show

232 eak ends and a phosphoesterase that trims 3'-oligoribonucleotide tracts until only a single 3'-ribonu

233 rhead ribozymes were synthesized from linear oligoribonucleotides using T4 RNA ligase.

234 RNA contact was not detected after a labeled oligoribonucleotide was released from the complex by nas

235 We found that the 27-base HIPBS oligoribonucleotide was sufficient to bind the protein i

236 3'-to-5' exoribonuclease specific for small oligoribonucleotides, was purified to homogeneity from e

237 ucleotides (dangling ends) at the termini of oligoribonucleotide Watson-Crick helixes (DeltaG(0)37,st

238 '- or 3'-hydroxyls of the terminal ribose in oligoribonucleotides, we have performed an extensive set

239 veral deletion RNA derivatives and synthetic oligoribonucleotides were constructed from the S segment

240 Thiouridine-containing oligoribonucleotides were used as 350 nm UV crosslinking

241 Three oligoribonucleotides were used to measure binding affini

242 The initiation site binds ATP or oligoribonucleotides, whereas the elongation site binds

243 Using a short oligoribonucleotide which contains all the necessary seq

244 The binding of EF-G to oligoribonucleotides with a U2653/C2667 double deletion

245 MicroRNAs (miRNAs) are endogenous oligoribonucleotides with exciting therapeutic potential

246 hat allows high-yield automated synthesis of oligoribonucleotides with pyrene incorporated at a speci

247 h ribozyme and substrate being two different oligoribonucleotides with regions of complementarity.

248 cleavage resynthesis steps, producing short oligoribonucleotides with uridine residues at the 3' ter