ライフサイエンスコーパス: ompA

1 The groEL-1, ompA, and omcB genes were used as markers for the early,

2 rological testing, outer membrane protein A (ompA) gene sequencing, and restriction fragment length p

3 he instability sequence also destabilized an ompA-bla fusion construct when fused to its 3'-UTR regio

4 reased half-lives of the rpsO, trxA, lpp and ompA transcripts.

5 dual full-length transcripts such as lpp and ompA varies significantly in wild-type cells.

6 pal-, lppA-, and ompA-deficient K. pneumoniae strains were constructed us

7 roles for Hfq: first, to bring both MicA and ompA into close proximity, and second, to restructure Mi

8 ipt (lpp) and two long transcripts (ompF and ompA).

9 ested PCR assays, targeting the 16S rRNA and ompA genes.

10 Polymorphism around ompA gene was associated with village-level trachomatous

11 t are encoded by different tandemly arranged ompA genes.

12 train-typing using one or two genes, such as ompA, does not correlate with clinical phenotypes.

13 een a history of intragenic recombination at ompA including one instance of interspecies recombinatio

14 the major outer membrane protein, encoded by ompA, has many functional constraints and is under purif

15 rroborate complex developmentally controlled ompA expression in C. trachomatis.

16 oters of late-stage transcribed genes: ctrA, ompA (peptidoglycan-associated lipoprotein), bolA (stres

17 ent, and spacer length of the Flavobacterium ompA promoter by measuring the effects of site-directed

18 For ompA, all but five isolates had sequence identical to th

19 .15% for the housekeeping genes to 12.1% for ompA.

20 The strongest bias was for ompA in strain D (P = 3.3 x 10(-8)).

21 around the C. trachomatis genome apart from ompA.

22 recombination are identified downstream from ompA, which encodes the major outer membrane protein.

23 Analysis of the C. gallinacea ompA phylogeny revealed at least 13 well segregated vari

24 he major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omc

25 served spotted fever group rickettsial gene (ompA) followed by DNA sequencing of the amplicon to iden

26 ysis of the key outer membrane protein genes ompA and ompF revealed that the corresponding gene trees

27 eeping genes and two membrane protein genes (ompA and espA) of 77 isolates that were diverse by PFGE

28 The promoters for two midcycle genes, ompA and pgk, were responsive to alterations in supercoi

29 nderstanding the transcription of gonococcal ompA through a regulatory system known to be important f

30 Greater ompA diversity at the district level was associated with

31 Greater ompA diversity at the district-level was associated with

32 GFP allows for the observation of changes in ompA expression in response to developmental signals.

33 based on nucleotide sequence differences in ompA, the gene that encodes the major outer membrane pro

34 We show that in many instances, ompA is a chimera that can be exchanged in part or as a

35 An isogenic ompA mutant showed reduced adherence compared to the par

36 LGV) recombinant strain possessing a non-LGV ompA genotype.

37 patients overexpressed nonsignificantly more ompA than those from nonbacteremic patients in the unice

38 Isolates from PP overexpressed more ompA than those from colonized patients from the unicent

39 Increased Ct infection in areas with more ompA variants requires longitudinal investigation to und

40 Additionally, we uncovered a new ompA promoter, which we named P3, utilizing the GFP repo

41 oter that is critical for MisR activation of ompA transcription.

42 PCR amplification of ompA followed by Southern blot detection of amplicands r

43 cts could be corrected by complementation of ompA.

44 In addition, expression of ompA during wild-type UTI was sharply increased at time

45 3 from the multicenter cohort) Expression of ompA was determined with quantitative reverse-transcript

46 e of recombination in the downstream half of ompA.

47 Outside of ompA, trachoma strains differed primarily in a very smal

48 etween biofilm formation and the presence of ompA gene among phage susceptible A. baumannii strains (

49 The region of ompA containing 13 tandem repeats was sequenced, reveali

50 Herein, we confirmed MisR/MisS regulation of ompA and report that the MisR response regulator can bin

51 , known to be involved in MicA regulation of ompA, may structurally remodel MicA to reveal the ompA-b

52 ly associated with some sequence variants of ompA and pmpEFGH.

53 genetic characterization of strains based on ompA, however, results in serovar groupings that are inc

54 s genetic diversity comes from serotyping or ompA genotyping, no quantitative assessment of genetic d

55 e encoding the major outer membrane protein (ompA) of Chlamydia trachomatis consists of a -35 hexamer

56 that encode immunodominant surface proteins (ompA and pmpEFGH) have been replaced by those characteri

57 icA is a small non-coding RNA that regulates ompA mRNA translation in Escherichia coli.

58 encing of six genes; 17-kDa, gltA, 16S rRNA, ompA, ompB, and sca4.

59 rollment and follow-up, 7 (20%) had the same ompA sequence at both visits, while 28 (80%) had discord

60 ate analysis in both cohorts together showed ompA overexpression as independent risk factor for pneum

61 mydia pneumoniae by a C. pneumoniae-specific ompA-based real-time PCR assay and 16S rRNA and 23S rRNA

62 signal sequences of beta-lactamase (bla SS), ompA, and phoA and the signal sequence and C-terminal pe

63 y PCR revealed that possession of two tandem ompA genes was widespread among this genus.

64 red to otherRickettsia-specific PCR targets (ompA,gltA, and the 17-kDa protein gene).

65 Given that ompA is highly conserved among Gram-negative pathogens,

66 Earlier work indicated that ompA is part of the MisR/MisS regulon and suggested that

67 These data suggest that ompA overexpression is an associated factor for pneumoni

68 Polymorphism around the ompA gene was associated with village-level trachomatous

69 OmpA- E. coli with a plasmid containing the ompA gene restored the ability of OmpA- E. coli to inhib

70 1% of all case samples) was positive for the ompA gene.

71 which has a topology quite distinct from the ompA tree.

72 tissue culture-based analyses implicate the ompA RNA thermometer as a bona fide S. dysenteriae virul

73 dependent self-association that occludes the ompA-recognition region.

74 can be accounted for by recombination of the ompA gene between different genomic backgrounds.

75 Disruption of the ompA gene in the tdcA mutant strain abolished the hypera

76 signed to target two variable domains of the ompA gene, VD2 and VD4.

77 into the 3' untranslated region (UTR) of the ompA gene.

78 e ppk gene showed increased stability of the ompA mRNA.

79 This defect in epithelial persistence of the ompA mutant was enhanced in competitive infections with

80 acer separating the -33 and -7 motifs of the ompA promoter also had a pronounced effect on promoter a

81 ntribution of a DNA sequence upstream of the ompA promoter that is critical for MisR activation of om

82 alter the temperature responsiveness of the ompA RNA thermometer has predictable consequences for bo

83 response regulator can bind upstream of the ompA translational start codon.

84 to restructure MicA to allow exposure of the ompA-binding site for pairing, thereby demonstrating the

85 insert had no effect on the half-life of the ompA-cat chimeric transcript.

86 may structurally remodel MicA to reveal the ompA-binding site for cognate pairing.

87 ains can be differentiated by sequencing the ompA gene encoding the outer membrane protein A (OmpA).

88 A primer set targeting the ompA gene (CP1-CP2/CPC-CPD) was used to perform a nested

89 hlamydiaceae: a multiplex test targeting the ompA gene and the rRNA intergenic spacer and a TaqMan te

90 By targeting the ompA gene, a real-time PCR assay has been developed to r

91 Microscopic examinations revealed that the ompA mutant formed significantly fewer IBCs, and those t

92 genetic analyses have demonstrated that the ompA RNA thermometer is a functional riboregulator suffi

93 site and increased ribosomal binding to the ompA transcript at permissive temperatures.

94 aumannii was decreased 2- to 3-fold when the ompA(Ab) gene was deleted.

95 c the conformational state of MicA where the ompA-binding site is exposed.

96 me extremely unstable in comparison with the ompA mRNA only when MazF(Sa) was induced in E. coli.

97 membrane protein (porB; 1,023 bp), with the ompA sequence (1,194 bp) used for reference.

98 ytophilum and Ehrlichia chaffeensis with the ompA, 17-kDa surface antigen gene, tsa56, msp2 (p44), an

99 newly identified RNA thermometer within the ompA transcript of Shigella dysenteriae First identified

100 ding amplification tests for C. trachomatis, ompA genotyping, and interviews and diary entries to doc

101 tain CtrA-binding motifs, and transactivated ompA, surE and bolA promoter-lacZ fusions in Escherichia

102 ng of the coding region of five transcripts, ompA, lpp, ompF, rpsA, and tufA.

103 The P. trehalosi ompA genes are highly diverged from those of M. haemolyt

104 emonstrate that whereas deletion of the UPEC ompA gene did not disrupt initial epithelial binding and

105 ing sequence and phylogenetic analysis using ompA and gltA gene fragments.

106 that predicting phylogenetic structure using ompA, which is traditionally used to classify Chlamydia,

107 izing element and was dominant to the 5'-UTR ompA and REP stability elements.

108 Transcriptional fusions with ompA and bla have been used to identify a novel mRNA ins