ライフサイエンスコーパス: one step

1 e stereogenic centers in yields up to 75% in one step.

2 dividual cells as well as their phenotype in one step.

3 ages and solving the Schrodinger equation in one step.

4 access to the densely substituted oxetane in one step.

5 analogues were prepared in 41-96% yields in one step.

6 to publication-ready results and figures in one step.

7 1-alkyl-1,2,3-triazoles ( rr up to 99:1) in one step.

8 kout (KIKO CAR-T cell) at high efficiency in one step.

9 ded a highly conjugated organic framework in one step.

10 chiral alpha-hydroxyaldehyde derivatives in one step.

11 nsitions shifting the cleavable phosphate by one step.

12 with control of three-dimensional shape, in one step.

13 isolate microorganisms in axenic culture in one step.

14 ld, and achieving a 99.7 % pure Dy sample in one step.

15 ubstituted indoles (up to 70% yield) in just one step.

16 c intermediate 8 as a single diastereomer in one step.

17 S can quantify IL-6 with high sensitivity in one step.

18 nes, and nitrogen-containing heterocycles in one step.

19 iminate unambiguously the fucose position in one-step.

20 LOV) methodologies is accomplished via rapid one-step 3D prototyping from digital models by user-frie

21 romiscuous biotin ligases in comparison with one-step affinity purification approaches.

22 la embryos to near homogeneity using a novel one-step affinity technique.

23 g of the guard allows the pathogen to remain one step ahead of the acquired immune response, enabling

24 Current models for DNA loop formation are one-step, all-or-nothing models with a looped state and

25 Besides, in one step, an antipode of the cephalosol intermediate nat

26 The azetidinols are easily synthesized in one step and can act as protecting groups for these phar

27 ylmethyl)amine (TPMA(3Cl) ) were replaced in one step and high yield by electron-donating cyclic amin

28 les the formation of 2D and 3D structures in one step and high yield.

29 quinoline alkaloids were readily prepared in one-step and high yields, highlighting the general appli

30 Herein, we demonstrate an easy operating one-step and low-cost way to synthesize egg-derived hete

31 Herein, we present a one-step and metal-free method for carbonyl deoxy-boryla

32 Both one-step and multistep well-designed quasistatic compres

33 cyclic iodonium ylide (SCIDY) precursors for one-step and regioselective radiofluorination, as well a

34 For this systematic review and one-step and two-step meta-analysis of individual partic

35 Here we introduce a contamination-free, one-step and universal Au-assisted mechanical exfoliatio

36 Each section is subdivided into direct (or one-step) and two-step labeling approaches and will star

37 5,7-fused ring system with an oxa-bridge in one step, and a stereoselective exo-Diels-Alder reaction

38 A one-step anion-exchange chromatography method (NaCl grad

39 g with rich edge sites are synthesized via a one-step approach by harnessing geometric constraints.

40 Here, a simple, cost-effective, and one-step approach that enables the control of cell orien

41 ere, we develop a facile, cost-effective and one-step approach to create an artificial lithium metal/

42 Herein we report a simple, one-step approach to prepare a low-cost and binder free

43 drogels has shown great promise, as a simple one-step approach, to generate multifunctional scaffolds

44 use of generalised linear mixed models in a one-step approach.

45 and bulk carbon nitride, was fabricated by a one-step approach.

46 ptimized lateral flow immunoassay strip, and one-step aqueous extraction.

47 ated, this primer concept was developed as a one-step assay, driven by PCR-amplified DNAzymes, for FO

48 quantify the levels of the two bacteria in a one-step assay, without the need for DNA extraction or a

49 ad and sample buffer, enabling essentially a one-step assay.

50 igh sensitivity is developed based on facile one-step assembled bio-nanocomposites with enzymes-loade

51 But these programs 'think' only one step at a time and have so far been limited to relat

52 nce dynamic programing enables the detection one step at a time, it is easy for researchers to trace

53 ce of alkali presents an efficient, general, one-step, atom-economic, and eco-friendly method for the

54 ia a facile pyrolysis-free route involving a one-step ball milling of the solid-phase-synthesized pol

55 correlations, which are degree correlations one step beyond those of neighboring nodes, we accuratel

56 Herein, a one-step build/couple/pair strategy that uniquely allows

57 naphtho-1,4-oxazinones have been achieved in one step by the formation of C-N, C-C, and C-O bonds.

58 feature of this biosensing platform is that one step calcination process is enough to create the fun

59 low carbon onions have been synthesized by a one-step carbonization of metal-organic-framework ZIF-67

60 Ferrocenes undergo one-step carboxamidation by reaction with isocyanates in

61 desired NAc-oligosaccharides by an efficient one-step chemical transformation.

62 ore/shell structures are synthesized using a one-step chemical vapor deposition process with rapid co

63 ng ARMC9 mutations as a cause of JS takes us one step closer to a full genetic understanding of this

64 amolecular (co)polymers and brings the field one step closer to applications.

65 his work takes microorganism-based analytics one step closer to field application in decentralized lo

66 k for haplotype-resolved SV calls that moves one step closer to fully resolved, diploid genomes.

67 This brings metabolomics one step closer to implementation into the clinic.

68 This approach takes us one step closer to mass production of macroscopic monola

69 This study brings us one step closer to new therapies intended to disable P.

70 s have recently been presented that bring us one step closer to this goal.

71 in single-cell omics has brought researchers one step closer to understanding the biological mechanis

72 role as a novel fibrotic-switch, bringing us one step closer to understanding the complex biological

73 brings the mainstream nitrite shunt and PN/A one step closer to wide applications.

74 functionalities and takes the nanocomposites one step closer toward future nanoscale device integrati

75 es with the current recommended dual therapy one step closer.

76 This work places the halide perovskite NCs one-step closer in designing the environmentally benign

77 metric wettability are synthesized through a one-step compartmentalized growth of a mesoporous organo

78 ions and candidate reaction paths point to a one-step concerted process, with the nucleophile playing

79 used for this novel synthesis is the facile one-step construction of a spiroketal trimer intermediat

80 he proposed cascade reaction allows a facile one-step construction of a tetracyclic isoindoloindole s

81 Moreover, one-step construction of imide and amide bonds with a lo

82 Direct C-H oxyfunctionalization, or the one step conversion of a C-H bond to a C-O bond, could b

83 Here we report an efficient one-step conversion of isolated mouse and human astrocyt

84 O/Al2O3@ZSM-5 core@shell catalyst active for one-step conversion of synthesis gas to dimethyl ether (

85 e, lysis, and assay workflow, we introduce a one-step copolymerization process that creates protein-d

86 ip test offered rapid detection as a simple (one-step), cost-effective, instrument-free assay and no

87 Here, we developed a one-step, cost-effective route to produce a novel biocom

88 A metal-free one step coupling reaction between various N-azole rings

89 Moreover, ZW800-1 is engineered for one-step covalent conjugatability, creating possibilitie

90 Application of our strategy using one-step CRISPR-Cas9 genome editing restructured vine-li

91 Here we report a one-step CRISPR/Cas9 KI methodology to combine the inser

92 likely owing to reduced inhibition with the one-step ddRT-PCR assay.

93 nditional and targeted-insertion alleles via one-step delivery of targeting components directly to zy

94 were enriched simultaneously, followed by a one-step denaturation, reduction, and alkylation.

95 We find that one-step deposition of the perovskite in the presence of

96 spersion, and 3) limitations associated with one-step deposition.

97 In contrast, we developed a one-step design strategy using phylogenetic analysis and

98 ctured electrodes and demonstrate the rapid, one-step detection of HIV-1 antibodies directly in clini

99 s the potential to be used for point-of-care one-step detection of norovirus in clinical samples.

100 Herein we report a simple and efficient one-step development of ultrasmall Cu(5.4)O nanoparticle

101 nti-HCV antibody-positive patients (known as one-step diagnosis) by analyzing all diagnostic tests pe

102 NA was more readily detected in solids using one-step digital droplet (dd)RT-PCR than with two-step R

103 Here, the one-step direct synthesis of 2D Mo2 C-on-graphene film b

104 ed hollow fiber membrane was developed via a one-step dry-jet wet spinning.

105 rchical bio-composite sensing film by facile one-step electro-deposition of 0D enzyme-polymer nanopar

106 Such ready-to-use biosensor would lead to a one-step electrochemical detection.

107 ous GaN layers that are obtained by a facile one-step electrochemical etching method without any extr

108 sulation approaches (layer-by-layer (LbL) or one-step electrodeposition (EcD)), layers composition an

109 re coated onto a screen-printed electrode by one-step electrodeposition and used to conjugate the HA

110 on films directly from silicon dioxide via a one-step electrodeposition process in molten salt for po

111 nosheets-polypyrrole (rGO@PPy) electrode via one-step electrodeposition technique.

112 Here, we develop and characterize a one-step enzymatic method to modify RNA 3' termini using

113 an alternative approach for an automated and one-step enzyme-linked immunosorbent assay (ELISA).

114 tor und molecular photocatalysts focusing on one-step excitation processes via single component photo

115 C) electrode with analysis following a short one-step extraction using ethanol.

116 phene artificial throat has the advantage of one-step fabrication, high efficiency, excellent flexibi

117 The TBP@PFBT PDs is a simple, one-step, fast, non-invasive, label-free, and inexpensiv

118 One-step fermentation proved to be a promising strategy

119 A one step formal [3+2]-annulation protocol for the synthe

120 This novel approach enables the one-step formation of CsPbI3/PEDOT composite, which coul

121 ose that the R213I mutation in RF2 brings us one step forward toward engineering an omnipotent RF in

122 single cell capture efficiency, we employ a one-step FRET-based biosensor which monitors the single

123 prepared under ambient conditions in one pot/one step from alkynes.

124 m sulfenamides, themselves readily formed in one step from amines and disulfides.

125 Thus, in one step from commercially available comonomers, our met

126 lized gamma-butyrolactones to be prepared in one step from simple tertiary alcohol-derived homoallyli

127 il and Cinnarizine, have been synthesized in one step from the commercial amines under this Mitsunobu

128 , Fe(II) clusters can be easily assembled in one step from the ligand-based reduction of the [Fe(II)(

129 In this work, we go one step further and search for links-entangled structur

130 In this work, we go one step further and we introduce chemical intuition in

131 We propose that this one step gene editing approach can be used to improve th

132 The one-step generated 0D-2D bio-composite sensing film demo

133 ody on the catalyst surface follows a simple one-step grafting from approach through radical polymeri

134 p synthesis of positively charged Pd NPs and one-step green synthesis of GQDs directly from graphite

135 One-step growth, reverse transcription-quantitative PCR,

136 We demonstrated its utility with one-step HHH purification of a wide range of traditional

137 -value products that can be obtained through one-step, high-yielding, catalytic transformations of th

138 A one-step, highly-efficiency, and low-cost cold atmospher

139 Here, we present a simple, yet efficient, one-step immobilization approach that is common for both

140 art microfluidic technology to demonstrate a one-step immunoassay for the detection of the cardiac ma

141 A one-step immunochromatographic strip test for LMG detect

142 structures into CQD films is demonstrated by one-step imprinting with well-controlled precision in bo

143 l drugs were prepared through this method in one step in 81-96% yield.

144 One step in disassembly is the release of the general tr

145 One step in this assembly in budding yeast is the associ

146 isubstituted 2,5-dihydrofurans are formed in one step in typically good yields and with good to excel

147 products with acetyl and carboxyl groups in one step, in good yields, and with short reaction times.

148 2v)-symmetric resorcin[4]arene tetraether in one step; in most cases, the single isomer can be precip

149 Our strategy entailed one-step injection of two gRNAs, Cas9 protein and a long

150 The development of practical, one-step installation and removal protocols further adds

151 introduce linked mutations simultaneously in one step into the mammalian germline has implications fo

152 This approach will facilitate the one-step introduction of gene constructs at a specific l

153 ation of the intermediates, whereby at least one step is catalyzed by an enzyme.

154 Here, we show how live E coli cell PCR and one-step LiCl-isopropanol purification can streamline DN

155 ion of antimicrobial nanoparticle systems, a one-step, light-driven procedure was developed to produc

156 ial derived quantum dots are synthesized via one-step liquid exfoliation method and biosurfactant was

157 tive electrokinetic concentration, we report one-step, liquid-phase NA purification that is simpler a

158 lity of DNA as a scaffolding material with a one-step lithographic process, we demonstrate the patter

159 Here, a one-step, low-cost, and mass-producible synthesis of pol

160 gold film electrode (GFE) are developed by a one-step, low-cost, straightforward, and mass-producible

161 , providing an ideal platform to explore the one-step magnetization reversal that is still conceptual

162 Transposon Analysis in Yeast (SATAY) allows one-step mapping of all genetic loci in which transposon

163 be better understood in terms of a two-stage one-step mechanism beyond molecular orbital consideratio

164 The one-step mechanism of these lithium cation Li(+)-catalyz

165 the reaction to proceed via an asynchronous one-step mechanism through a zwitterionic species.

166 of the primary and secondary endpoints using one-step meta-analysis only.

167 FAIRS is a general, one-step method and is naturally specific in detection.

168 Recently, a unique one-step method for directly fabricating green and brown

169 his novel design of PNA microcapsule and the one-step method of cell encapsulation can be a versatile

170 Here we describe an IP-free one-step method that enables stable genome integration i

171 nocomposites were synthesized by an in-situ, one-step method without the use of a reducing agent.

172 We also provide a one-step-method to achieve high quality predictions for

173 the lipid extraction step, these simplified one-step methods offer a much higher throughput and a re

174 is of BMP and PG species was developed after one-step methylation of lipid extracts in combination wi

175 s the main subject of this paper, features a one-step Michael addition of t-butyl 2-((diphenylmethyle

176 th amine moieties were synthesized using the one-step microwave pyrolysis of citric acid in the prese

177 Here, we show that homogeneous, one step (mix-and-read) TFA methods can be extended to t

178 ytidine was used as an internal standard and one-step multi-enzyme digestion was used to release the

179 tegrated into a microfluidic chip, achieving one-step multiplex analysis within 40 min.

180 pecialized bis-PNP macrochelate that enables one-step Ni(II) binding to a p-Si(111) substrate.

181 (18)F-JNJ-64413739 was radiolabeled via a one-step nucleophilic aromatic substitution.

182 cuticles was obtained biotechnologically in one step of enzymatic deproteinization and after deacety

183 One step of the mating sequence regulated by these neuro

184 N-sulfinyltritylamine (TrNSO), available in one step on 10 gram scale, as a linchpin.

185 d VT behavior for the four cobalt complexes; one-step one-electron partial VT, two-step VT, incomplet

186 he coexistence of sexual recombination and a one-step operation in the domestication of clonally prop

187 xture, while 'Singapore Spanish' supported a one-step operation of domestication.

188 from South America was hypothesized to be a 'one-step operation'.

189 ents and can be carried out through a simple one-step operation.

190 Cas12a, and nCas9-Traget AID) using either a one-step or a two-step transfection platform to obtain t

191 eous FAU-type zeolite, HOU-3, prepared via a one-step organic-free synthesis route.

192 ed with either the CRM-based adhesive (CBA), One-Step (OS; Bisco, Inc.), or a negative adhesive (NA)

193 We observe classical one-step pathways and nonclassical two-step pathways tha

194 tion of the device, which could be used as a one-step, portable, disposable, low-cost, simple, instru

195 Herein, we report a biomimetic, one-step preparation of archazolid F, the most potent an

196 The novel, simple and straightforward one-step printing process for fabrication of a biosensin

197 xide electrocatalysts, which were derived in one-step procedure from oriented surface-mounted metal-o

198 This one-step procedure involves treatment of anilines with N

199 We have developed a one-step procedure to a variety of chiral lipophilic and

200 We report a one-step procedure to directly reduce unactivated aryl e

201 odels (GLMMs), straightforward and efficient one-step procedures for fitting SSFs with random coeffic

202 This efficient one-step process addresses several limitations of the tr

203 We found that large-scale contraction is a one-step process, resulting in a median loss of ~60 trip

204 ctional antibody-drug conjugates (ADCs) in a one-step process.

205 rting molecules of different ring sizes in a one-step process.

206 i-step process to loop the DNA rather than a one-step process.

207 for harmful chemicals and offering simpler, one-step, process control.

208 ynergistic sorbent separation method for the one-step production of polymer-grade C(2)H(4) from terna

209 lid-phase assay, or microfluidic assays) for one-step protein quantification.

210 Subsequently, a simple one-step protocol was established for the purification a

211 ction is another practical advantage of this one-step protocol.

212 panga reagent not only allows its convenient one step purification but also extends its use for detec

213 using a bacterial overexpression system and one-step purification.

214 -scaled ADMS-decorated carbon networks via a one-step pyrolysis.

215 separation efficiency of Nd/Sm system and a one-step quantitative separation of Nd/Dy with the aid o

216 The new one-step QuEChERS strategy was compared to other methodo

217 A one-step QuEChERS-based approach to extraction and clean

218 are distinguished rapidly and accurately via one-step radical-induced dissociation.

219 aceuticals and PET radioligands, including a one-step radiosynthesis of the clinically used compounds

220 terial, and the enzyme was immobilized via a one step rapid and simple procedure that does not requir

221 The procedure utilizes a simple one-step reaction, with the derivatising agent N-methyl-

222 This method achieves one-step, redox-neutral synthesis of catechols with dive

223 anoparticles (Tyr-Au NPs) were fabricated by one-step reduction of Au(3+) ion using Tyr as a reducing

224 ane separation could lower the energy input, one-step removal of multiple impurities, especially trac

225 the mineralization mechanism allows a simple one-step route for fabricating functionally graded mater

226 We report a one-step route to [(18)F]CF(3)SO(2)NH(4) from [(18)F]flu

227 The method provides a mild, one-step route to [carbonyl-(13/14)C] alkyl amides, ther

228 vskite reprecipitation at low energy cost, a one-step scalable method is developed to produce freesta

229 iodic table, their compounds, or alloys by a one-step, scalable, low-cost, and eco-friendly process.

230 II secretion system (T3SS), which engages in one-step secretion of effectors(4), despite possessing a

231 e surface of infected cells, allowing facile one-step selection with streptavidin-conjugated magnetic

232 ne Oxide (rGO) based impedimetric sensor for one step sensing of DNA.

233 h, and the process represents an interesting one step separation that could be used in an (225)Ac gen

234 Finally, a one-step separation method (SF > 3 x 10(6) and radiopuri

235 cone symmetry restricted to be two-fold, the one-step sharp magnetization reversal is realized and gi

236 This one-step "signal on" biosensor takes 20min for the sensi

237 rk (magOTfP5SOF-Ga(3+)) was designed for the one-step simultaneous enrichment of glycopeptides and ph

238 Here, SNZVI synthesized by either a one-step (SNZVI-1) or two-step (SNZVI-2) process were ch

239 TPMCalculator is a one-step software to process RNA-seq alignments in BAM f

240 was aimed at investigating the use of a fast one-step solid-liquid extraction procedure followed by a

241 lly aligned polymer chains by using a simple one-step solution-shear/bar coating technique.

242 rganic frameworks (sMOFs) are obtained via a one-step solvothermal approach rather than in a layer-by

243 re synthesized in situ on graphite felt by a one-step solvothermal process, can significantly boost e

244 ntrast agent (perfluoropentane, PFP) using a one-step sonoporation method to create ELTSLs and ENTSLs

245 The one-step spin-coating process of perovskites is investig

246 on mechanism of 2D halide perovskites during one-step spin-coating.

247 Here, a one-step strategy for fabricating core/shell poly(vinyli

248 Thus, oligolysine-PEG is a one-step, structure-independent approach that provides l

249 In this work, a simple one-step surface modification method using 3-aminopropyl

250 s were simply immobilized on paper through a one-step surface modification process.

251 affolds can be implanted via a cell/GF-free, one-step surgery for in situ bone regeneration, thus dem

252 The one-step syntheses of exotine A and exotine B, which com

253 The one-step syntheses, X-ray structures, and spectroscopic

254 Herein, a novel one step synthesis of multicomponent three dimensional p

255 tive polymer nanocomposite was obtained by a one-step synthesis approach based on room temperature di

256 aining three stereocenters were prepared via one-step synthesis in yields ranging from 88 to 96% and

257 a novel method for the simple and efficient one-step synthesis of 1,2,4-triazines under mild conditi

258 A one-step synthesis of 4-alkyl phthalates was derived fro

259 We designed and developed a one-step synthesis of 5-amino-4-carboxamidothiazoles 1 b

260 In this work, we present a highly selective one-step synthesis of a formaldehyde derivative starting

261 A one-step synthesis of a nanographene propeller with a D3

262 This methodology was adapted for the one-step synthesis of a series of clinically active topo

263 A novel one-step synthesis of anthraquinones and chloro anthraqu

264 A general asymmetric route for the one-step synthesis of chiral beta-branched amides is rep

265 Now, a one-step synthesis of metal-inorganic frameworks Hf(4) N

266 Herein, we describe a one-step synthesis of N,N-dicarbamoyl 2-iminothioimidate

267 In this paper, we report a simple one-step synthesis of well-dispersed amorphous cobalt hy

268 that the properties of the SNZVI made from a one-step synthesis procedure may provide better reactivi

269 This study reports a room-temperature and one-step synthesis technique that leads to large-scale a

270 Utmost availability (one-step synthesis without chromatographic purification)

271 This practical and scalable method provides one-step synthetic access to primary anilines and phenol

272 ) as a catalyst was found to be an efficient one-step synthetic route to 5-iodo-4-ethynyltriazoles.

273 BLI-SELEX is a one-step technique for rapidly generating aptamers again

274 Here, we firstly developed a "one-step" tetra-primer amplification-refractory mutation

275 a wide range from 20 to 1000 nm followed by one-step thermal activation.

276 Here we report a one-step three-component radical coupling of [1.1.1]prop

277 In Experiment 1, we constrained one step to be markedly shorter or longer than preferred

278 simple and scalable, and provides access in one step to high-value building blocks for application i

279 the E2 or E3 enzyme and then transferred in one step to the substrate.

280 s have been successfully combined in one-pot one-step to allow functionalization of a large variety o

281 eicosanoic acids caused a transition from a one-step to two-step process.

282 ing the age to start screening are imminent, one step toward integrating screening and vaccination.

283 wo models for this process: "gobbling," i.e. one-step transfer of all HDL-CE to the cell and "nibblin

284 magnetic bead-based extraction of NAs with a one-step transfer to dNAAT.

285 system I(2)/dimethyl sulfoxide mediates the one-step transformation of alpha-isopropylidene ketones

286 phosphonates were demonstrated in different one-step transformations.

287 four [(C-C)-(C-N) and (C-C)-(C-O)] bonds in one step under single catalytic conditions.

288 active template synthesis of [2]rotaxanes in one step, up to 95% yield and >100:1 rotaxane:axle selec

289 -purin-2-(3H)-ones 25-26 were synthesized in one step using formic acid, urea, guanidine carbonate, a

290 o obtain seven-membered ring benzosultams in one step, using saccharin anion as starting material.

291 nic droplet screen approach is developed for one-step washing-free multiplex detection of single-cell

292 As one step, we have addressed the feasibility of detecting

293 Herein, a novel one-step wet-chemical method is first proposed for prote

294 -value molecules from feedstock chemicals in one step while consuming only photons.

295 the chirality of five stereogenic centers in one step with 96% ee.

296 self-healing materials were manufactured in one step without any needs for any sequential stages, i.

297 ve-cell membrane proteins can be achieved in one step without any pre-treatment.

298 ee-dimensional paper microfluidic devices in one step, without the need of stacking layers of paper,

299 This study proposed a one-step wood modification method by combining the depos

300 Altogether, our study confirms that one-step zygotic injections of CRISPR/Cas gene editing c