ライフサイエンスコーパス: optical mapping

1 Activation was recorded with optical mapping.

2 short-, linked- and long-read sequencing and optical mapping.

3 subtracted) during AF in vivo and in ex vivo optical mapping.

4 tle fine-scale Ca(2+) alternans, captured by optical mapping.

5 multidimensional photography through active optical mapping.

6 ective myocardial conduction was detected by optical mapping.

7 ysis was performed on each preparation after optical mapping.

8 icular conduction velocity, as determined by optical mapping.

9 mice using high-resolution, dual-wavelength optical mapping.

10 typic sequenced strain by using the tools of optical mapping.

11 acteristics of the PVs using high-resolution optical mapping.

12 tential plateau, and DeltaVm was measured by optical mapping.

13 strain 2.4.1 were constructed using shotgun optical mapping.

14 ships were assessed using immunostaining and optical mapping.

15 which utilize molecular fixation, including optical mapping.

16 conformation capture (Hi-C) sequencing, and optical mapping.

17 fingerprinting, radiation hybrid mapping and optical mapping.

18 g electrocardiography, echocardiography, and optical mapping.

19 derived cardiac cell sheets (hiPSC-CCSs) and optical mapping.

20 n heart rate, as measured by ECG and ex vivo optical mapping.

21 d the results by whole genome sequencing and optical mapping.

22 of long-range sequencing and single-molecule optical mapping.

23 ule real-time sequencing and high-resolution optical mapping.

24 al locations on the molecules generated from optical mapping.

25 ce alignment, with modifications specific to optical mapping.

26 ivation spread recorded simultaneously using optical mapping.

27 nesthetized Thy1-GCaMP mice using wide-field optical mapping.

28 recording depolarization in the scar through optical mapping.

29 We addressed this gap with Optical Mapping, a high-throughput, high-resolution sing

30 ip system has drawn from critical aspects of Optical Mapping, a single-molecule system that enables t

31 Here, we describe how optical mapping, a single-molecule system, was used to i

32 In this study, we present the application of optical mapping, a single-molecule, whole-genome analysi

33 hromosome 2 DNA as the starting material for optical mapping, a system for making ordered restriction

34 ides a fast, simple alternative to the large optical mapping analysis programs currently available fo

35 s were removed during the integration of the optical mapping and chromosome conformation capture data

36 Despite their technical differences, optical mapping and chromosome conformation capture perf

37 itored voltage and Ca(2+) transients through optical mapping and compared them to their isogenic cont

38 egrating ultrastructural imaging, long-range optical mapping and computational analysis of whole-geno

39 se assemblies, we generated BioNano Genomics optical mapping and Dovetail Genomics chromosome conform

40 Finally, using optical mapping and electrophysiological analyses, we pr

41 c animals and incorporating information from optical mapping and fiber-FISH.

42 nding (TAB) was used for ex vivo whole heart optical mapping and for Ca(2+) and reactive oxygen speci

43 Here we used optical mapping and Fourier analysis to determine the di

44 ethod relieves a long-standing limitation of optical mapping and has potential to enhance new studies

45 ingle-molecule real-time sequencing, BioNano optical mapping and high-throughput chromosome conformat

46 We applied biatrial optical mapping and immunoblot mapping of various atrial

47 Optical mapping and immunohistochemistry indicate that p

48 amera technology have increased the power of optical mapping and made a major impact on the field of

49 Electrophysiological effects were studied by optical mapping and patch-clamping.

50 in the intact non-failing rabbit heart using optical mapping and pharmacological manipulation of RyRs

51 e isolated rabbit heart experimentally using optical mapping and superconducting quantum interference

52 We perform optical mapping and transmission electron microscopy in

53 Using ratiometric optical mapping and video microscopy, we discovered that

54 ent Na(+)-channel block mathematical models, optical mapping, and action potential recording were use

55 able by pulsed-field gel electrophoresis and optical mapping, and harbored a novel pmrC1A1B allele.

56 ntegrating PacBio, 10x Chromium, BioNano DLS optical mapping, and Hi-C mapping technologies.

57 In vivo heart rhythm telemetry, optical mapping, and isolated cardiac myocyte imaging we

58 d Pitx2c mRNA by electrophysiological study, optical mapping, and patch clamp studies.

59 ad, strand-specific sequencing technologies, optical mapping, and variant discovery algorithms to com

60 Using a new optical mapping approach, we measured action potentials

61 study, using next-generation sequencing and optical mapping approaches, a 24.1-Mb complete genome of

62 Thanks to its all-passive optical mapping architecture, the ATOM display features

63 oval, our algorithm improved the accuracy of optical mapping, as demonstrated by spatial maps of calc

64 natal rat ventricular myocytes and performed optical mapping at high temporal and spatial resolution.

65 In optical mapping, at 4 mM [K(+)](o), 17 null hearts showe

66 We present an all-passive, transformable optical mapping (ATOM) near-eye display based on the "hu

67 Using in vivo optical mapping/calcium imaging, we determined that the

68 We discuss how optical mapping can be used as a validation tool for gen

69 Given the rapid spread of the use of optical mapping, careful evaluation must be made in term

70 f long QT syndrome type 2 using intact heart optical mapping, cellular electrophysiology and confocal

71 We used epicardial and endocardial optical mapping, chemical subendocardial ablation with L

72 Subsequent optical mapping confirmed a DF gradient from posterior L

73 Optical mapping confirmed that treatment with each compo

74 e and interact with the alignment of Bionano optical mapping data and can be used for in depth explor

75 We generated and used stimulated optical mapping data for loblolly pine and F.tularensis

76 loblolly pine and F.tularensis and used real optical mapping data for rice and budgerigar.

77 Analysis of lacZ expression and optical mapping data highlight important differences bet

78 Optical mapping data is used in many core genomics appli

79 Analysis of optical mapping data reveals that VF excitation frequenc

80 The optical mapping data were in good agreement with theory.

81 from the sequence data were compared to the optical mapping data, and good correspondence was found.

82 for low spatial resolution from downsampled optical mapping data, validated against high-resolution

83 Using the real optical mapping data, we correctly identified 75% of ext

84 akpoints using paired-end sequence reads and optical mapping data.

85 n source computational methods for analyzing optical mapping data.

86 re few publicly available tools for aligning optical mapping datasets.

87 uent ventricular ectopy, and/or bigeminy and optical mapping demonstrated high prevalence of spontane

88 Optical mapping demonstrated that current delivered with

89 sing CLC Genomics Workbench read mapping and Optical mapping developed by OpGen.

90 Simultaneous endocardial and epicardial optical mapping (di-4-ANEPPS) was performed in isolated,

91 Optical mapping (Di-4-ANEPPS, 300 frames/sec) of the pos

92 In preparations with z(0) <700 microm, optical mapping during epicardial stimulation revealed u

93 Optical mapping during VF showed that activity often beg

94 ufficient mice on which patch-clamp studies, optical mapping, electrocardiogram analyses, and ischaem

95 High-resolution optical mapping employing seven enzymes places these clo

96 Optical mapping enabled spatial characterization of exci

97 assive number of individual DNA molecules by optical mapping enables assembly of physical maps spanni

98 Optical mapping experiments allow investigators to view

99 METHODS AND Biochemical, patch clamp, and optical mapping experiments demonstrate that PKP2 associ

100 Finally, optical mapping experiments in AnkG-silenced cells demon

101 We performed optical mapping experiments in isolated Langendorff-perf

102 Here, we simulate dual voltage-calcium optical mapping experiments using a monodomain-Luo-Rudy

103 Optical mapping experiments were performed on Langendorf

104 In simulations and optical mapping experiments, virtual PentaRay recordings

105 maging and enriched the model with data from optical mapping experiments.

106 combination of microelectrode recordings and optical mapping experiments.

107 egration locus, demonstrating the utility of optical mapping for the analysis of genomic regions that

108 and by abolishing contraction, also prevent optical mapping from being used to study coupling betwee

109 Optical mapping has become an indispensible tool for stu

110 In this study, optical mapping has been used to elucidate LCR22 structu

111 Cardiac optical mapping has proven to be a powerful technology f

112 Recent advances in the automation of optical mapping have enabled us to map a set of 16 BAC c

113 microelectrode recordings and more recently optical mapping have ushered in new periods of significa

114 and in situ, and mechanisms were defined by optical mapping, histochemistry, and biochemistry.

115 racardiac electrophysiology, high-resolution optical mapping in atrial preparations, and patch clampi

116 estion, we conducted simultaneous voltage/Ca optical mapping in atrial tissue and one-/two-dimensiona

117 mon understanding of the basic principles of optical mapping in complex 3D anatomic structures.

118 tive nNav1.6 blockade during high-resolution optical mapping in explanted human hearts depress intran

119 ls) cells, biochemistry, dual Ca(2+)/voltage optical mapping in intact hearts from alcohol-exposed or

120 METHODS AND Using optical mapping in isolated perfused canine atrial prepa

121 rther confirmed by electric measurements and optical mapping in Langendorff-perfused hearts.

122 propagation was assessed by high-resolution optical mapping in monolayers of neonatal rat ventricula

123 By allowing high-resolution optical mapping in the absence of electromechanical unco

124 iefly look into the possible future roles of optical mapping in the development of regenerative cardi

125 A good example of this is the use of optical mapping in the sinoatrial node (SAN): when micro

126 Optical mapping is a new tool that creates a high-resolu

127 Optical mapping is a technique for capturing fluorescent

128 Bionano optical mapping is a technology that can assist in the f

129 Optical mapping is an accessible and relatively mature t

130 Optical mapping is an emergent technology for genomic an

131 r study that may be used, until such time as optical mapping is clinically feasible, to improve mecha

132 lysis which may facilitate the automation of optical mapping is presented.

133 An important limitation of conventional optical mapping is that contraction is usually arrested

134 Panoramic optical mapping is the primary method for imaging electr

135 Traditional optical mapping lacks the spatial resolution to assess c

136 but MI differences are amplified by current optical mapping methods.

137 In optical mapping, molecules are elongated and fixed onto

138 long-read re-sequencing (N = 31), as well as optical mapping (N = 16), we apply both assembly- and re

139 gated using a combination of high-resolution optical mapping (n = 5) and extracellular bipolar and in

140 underlying PV tachycardias was confirmed by optical mapping (n = 5).

141 man atria (n=11) by subsurface near-infrared optical mapping (NIOM; 0.3 mm(2) resolution) and 64-elec

142 By using high-resolution optical mapping, numerical simulations and metaanalysis

143 We used optical mapping of action potentials and ratiometric cal

144 Notably, optical mapping of action potentials in atrial fibrillat

145 Here, we make progress by combining optical mapping of action potentials with concurrent act

146 Optical mapping of arrhythmias and defibrillation provid

147 Optical mapping of Ca(2+) transients revealed that rat A

148 t this hypothesis, we performed simultaneous optical mapping of Ca(i) and membrane potential (V(m)) i

149 Optical mapping of cardiac electrical activity using a v

150 So far, the optical mapping of cardiac electrical signals using volt

151 he excitation and emission of photons during optical mapping of cardiac tissue.

152 Optical mapping of chimeric hearts by use of voltage-sen

153 Optical mapping of coronary-perfused atria (n=24) reveal

154 Novel intramural optical mapping of coronary-perfused right atrial prepar

155 Optical mapping of cytosolic calcium transients in intac

156 Optical mapping of DeltaSIV hearts revealed that ventric

157 diagnostic applications, DNA sequencing and optical mapping of epigenetic markers.

158 While over the last two decades optical mapping of excitation (voltage and calcium dynam

159 This enables the optical mapping of fluidity and porosity patterns in tar

160 Thus, optical mapping of genetically engineered mice may help

161 Optical mapping of HFpEF hearts demonstrated prolonged a

162 for persistent atrial fibrillation (AF) from optical mapping of human atria and clinical studies of A

163 Optical mapping of intact cardiac tissue reveals that, i

164 or improved interpretation of the results of optical mapping of intact heart preparations.

165 In vivo ECG recording and whole heart optical mapping of intact hearts was performed before an

166 Optical mapping of intracellular Ca (Ca(i)) and voltage

167 Simultaneous optical mapping of intracellular Ca(2+) and membrane pot

168 At 4 weeks, optical mapping of intracellular Ca2+ and ROS was perfor

169 In vitro, high-resolution near-infrared optical mapping of intramural SAN activation was perform

170 neonatal cell pairs from mutant hearts, and optical mapping of isolated-perfused hearts with voltage

171 Simultaneous voltage-calcium optical mapping of Langendorff-perfused SHR hearts revea

172 Optical mapping of linearized DNA molecules is a promisi

173 Optical mapping of membrane voltage (V(m)) and intracell

174 y ganglia in vivo, opening the door to rapid optical mapping of neuronal excitability.

175 s of AF drivers in a 2-dimensional model and optical mapping of ovine atrial scar-related AF.

176 Optical mapping of perfused hearts confirmed slowed cond

177 High-throughput optical mapping of several hundred intra-chromosomal int

178 Optical mapping of the epicardial electrical activation

179 ion potential duration using high-resolution optical mapping of the epicardial surface in 8 isolated,

180 Optical mapping of the epicardial surface in ventricles

181 Detailed optical mapping of the epicardial surface of guinea pig

182 Optical mapping of the first extrabeat with a coupling i

183 ultaneous voltage and intracellular Ca(+)(2) optical mapping of the left ventricular epicardial surfa

184 Conduction velocity measurements from optical mapping of the RBB revealed slow conduction in C

185 We used simultaneous optical mapping of the right and left atria (RA and LA)

186 Optical mapping of the SQTS-hiPSC-CCSs revealed shortene

187 cts of ISO on LQT1 and LQT2 were verified by optical mapping of the whole heart, suggesting that ISO-

188 Simultaneous optical mapping of transmembrane potential and Ca(2+) tr

189 To validate this model, we used optical mapping of transmembrane potential and calcium t

190 Optical mapping of transmembrane potential and intracell

191 ocity, measured by multisite high-resolution optical mapping of transmembrane potential in strands of

192 ping ventricular myocardium using high-speed optical mapping of transmembrane potentials and calcium

193 Optical mapping of transmembrane voltage and pacing stud

194 formed, and a 4-cm2 area of the PV underwent optical mapping of transmembrane voltage to obtain 256 s

195 In addition, comparative optical mapping of two other inbred maize lines revealed

196 Optical mapping of uniformly Kir2.1 gene-modified monola

197 Optical mapping of V(m) and Ca(i) in paced rabbit hearts

198 We used optical mapping of V(m) in patterned cultures of neonata

199 Simultaneous optical mapping of Vm (with RH237) and [Ca(2+)]SR (with

200 Simultaneous optical mapping of voltage and [Ca(2+)]i in CPVT hearts

201 Programmed electric stimulation ex vivo and optical mapping of voltage transients indicated that pep

202 AmpliconReconstructor (AR), for integrating optical mapping (OM) of long DNA fragments (>150 kb) wit

203 ong-range genome analysis platforms, such as optical mapping, one can identify large SVs (>2 kb) acro

204 g in vivo electrophysiology, high-resolution optical mapping, patch clamping, and molecular biology.

205 e analysis and further tested in previous VF optical mapping recordings of coronary perfused donor he

206 nformation from the shape of the upstroke in optical mapping recordings.

207 egmentation and tracking of cardiac waves in optical mapping recordings.

208 Optical mapping revealed a loss of the normal elliptical

209 Optical mapping revealed cardiac refractoriness with los

210 Optical mapping revealed prolonged action potential dura

211 Optical mapping revealed significant rate-dependent RA c

212 solated, Langendorff-perfused rabbit hearts, optical mapping revealed that dofetilide-induced arrhyth

213 Optical mapping revealed that selectively the transverse

214 ifferences similar to variations in I(Ca,L); optical mapping revealed that the earliest EADs fired at

215 and long-read sequencing in conjunction with optical mapping, revealed a compact genome of approximat

216 First, we explore key components of a modern optical mapping set-up, focusing on: (1) new camera tech

217 Optical mapping showed increased activation in the borde

218 Optical mapping showed significant conduction slowing in

219 High-resolution optical mapping showed that epicardial wavefront expansi

220 Optical mapping showed that this effect also applied to

221 phasic action potentials and to conventional optical mapping signals.

222 f electrical shocks within the heart, recent optical mapping studies have revealed two major discrepa

223 Optical mapping studies have suggested that intracellula

224 Optical mapping studies in mice demonstrate that ANP spe

225 We then look at recent optical mapping studies in single cells, cardiomyocyte m

226 Electrophysiological and optical mapping studies indicated high-grade atrioventri

227 Recent optical mapping studies of cardiac tissue suggest that m

228 Moreover, optical mapping studies of embryonic hearts demonstrate

229 We performed optical mapping studies of VF in isolated swine right ve

230 Optical mapping studies showed that during rapid ventric

231 zation of the large-insert clones, e.g., for optical mapping studies.

232 y, intracardiac electrograms and pacing, and optical mapping studies.

233 Optical mapping surfaces have been shown to be compatibl

234 The Optical Mapping System discovers structural variants and

235 The optical mapping system reported here is based on our phy

236 A multisite optical mapping system was used to obtain 17 or 25 micro

237 tin using either a confocal microscope or an optical mapping system.

238 Tandem-lens optical mapping systems facilitated simultaneous recordi

239 dvances, we developed and validated low-cost optical mapping systems for panoramic imaging using Lang

240 Optical mapping technique was used to assess effects of

241 ed preparations of left ventricle (LV) by an optical mapping technique.

242 in geometrically defined cell cultures using optical mapping technique.

243 hock-induced DeltaV(m) was measured using an optical mapping technique.

244 s in DNA repair, were directly visualized by optical mapping techniques after gamma irradiation.

245 studied ventricular defibrillation by use of optical mapping techniques failed to observe an initial

246 We used optical mapping techniques to study the effects of propr

247 Optical mapping techniques were used to determine the pa

248 To address these mechanisms, high-resolution optical mapping techniques were used to measure action p

249 Imaging methods utilizing optical mapping techniques will need to account for thes

250 By optical mapping techniques, action potential duration (A

251 Using optical mapping techniques, calcium transients and actio

252 g voltage-sensitive dyes and high resolution optical mapping techniques, we found that uncoupling of

253 ion and contraction; (6) new multiparametric optical mapping techniques; and (7) photon scattering ef

254 ating the potential benefit of more accurate optical mapping technologies, such as nano-coding.

255 Previously, we used new optical mapping technology to image VF wavefronts from n

256 dated (AGPv4) using long-read sequencing and optical mapping technology.

257 e compared to sham mice by echocardiography, optical mapping, telemetry electrocardiographic monitori

258 onstrate the use of technology developed for optical mapping to acquire DNA fingerprints from single

259 , we first use 10x Genomics linked-reads and optical mapping to assemble and annotate a nearly chromo

260 icroscopy, immunochemistry, patch-clamp, and optical mapping to assess the interactions between AnkG,

261 We used Optical Mapping to create genome-wide restriction maps o

262 We used optical mapping to measure the effects of baroreflex, ch

263 We used optical mapping to reconstruct patterns of activation du

264 The applicability of optical mapping to this problem was enhanced by advances

265 This article highlights the use of optical mapping, to detect and correct assembly errors i

266 Cardiac optical mapping uses potentiometric fluorescent dyes to

267 Optical mapping using voltage-sensitive dye revealed slo

268 Optical mapping using voltage-sensitive fluorescent dyes

269 By contrast, panoramic optical mapping, using fluorescent dyes, offers high spa

270 Thus optical mapping was advanced to use simply extracted, un

271 Optical mapping was performed in 44 rat ventricular myoc

272 Ventricular fibrillation (VF) optical mapping was performed in Langendorff-perfused Sp

273 Optical mapping was performed in zebrafish hearts to det

274 Optical mapping was performed on epicardial, endocardial

275 Optical mapping was performed to evaluate diastolic spon

276 Optical mapping was used to assess bidirectional conduct

277 Optical mapping was used to measure action potential dur

278 High-resolution optical mapping was used to measure conduction velocity

279 High-resolution optical mapping was used to study conduction in the righ

280 Optical mapping was used to study the electrophysiologic

281 Using optical mapping we observe increased dispersion of actio

282 Using fiber FISH and Bionano optical mapping, we assembled LCR22 alleles in 187 cell

283 Through active optical mapping, we demonstrate our approach in two nich

284 using optical mapping, we investigated the effects of I(Kr) up

285 Illumina, 454 sequencing, and optical mapping were used to obtain a complete genome se

286 Whole-genome optical mapping (WGM) (OpGen, Inc.) was performed on sel

287 Whole-genome mapping (formerly optical mapping), which is a high-resolution ordered res

288 pplication of spaced seeds in the context of optical mapping, which allows for spurious and deleted c

289 ventricular arrhythmias (VA) were probed by optical mapping, whole-cell patch clamp to measure actio

290 High-resolution optical mapping with correlative histological analysis o

291 Can the integration of panoramic optical mapping with electrical mapping overcome the lim

292 etic and optogenetic fluorescent probes; (5) optical mapping with motion and contraction; (6) new mul

293 Chromosome 2 was found to be 976 kb by optical mapping with NheI, and 946 kb with BamHI, which

294 Optical mapping with the voltage-sensitive dye di-4 ANEP

295 d variation by integrating our findings from optical mapping with those from DNA sequencing-based gen

296 Dual optical mapping with voltage- and Ca(2+) -sensitive dyes

297 All hearts were Langendorff-perfused for optical mapping with voltage- and Ca(2+)-sensitive dyes

298 Optical mapping with voltage-sensitive dyes provides a h

299 Using high-resolution optical mapping with voltage-sensitive dyes, we measured

300 ing of impulse propagation, as determined by optical mapping with voltage-sensitive dyes.