ライフサイエンスコーパス: optical trap

1 out influences from optical gradient forces (optical trapping).

2 ct-induced phase changes being studied in an optical trap.

3 lity but increased force production using an optical trap.

4 he stroke size, as observed with a dual-beam optical trap.

5 sitioned near microtubule plus ends using an optical trap.

6 es under the low-force regime afforded by an optical trap.

7 es them well-suited for manipulation with an optical trap.

8 from the microtubule under the force of the optical trap.

9 ep size under applied load as measured in an optical trap.

10 nating hindering and assisting loads with an optical trap.

11 an elastic lift force upon release from the optical trap.

12 evels when external tension is applied by an optical trap.

13 ment kinetics with varying tensions using an optical trap.

14 rque application and detection in an angular optical trap.

15 .6 degrees C/100 mW in a nonheating (830 nm) optical trap.

16 ulating the input polarization in a standard optical trap.

17 eosomes in a single chromatin fiber using an optical trap.

18 ngraving on a gold film is considered as the optical trap.

19 s of the size typically present in a magneto-optical trap.

20 motors can generate over 8 pN of force in an optical trap.

21 ndering or assisting mechanical loads in the optical trap.

22 en DNA handles that are attached to beads in optical traps.

23 armonic potential trap analogous to atoms in optical traps.

24 sing arrays of time-multiplexed, holographic optical traps.

25 at both ends by microspheres in two separate optical traps.

26 he loading efficiency of (209,210)Fr magneto-optical traps.

27 ached to two beads that were held in the two optical traps.

28 l structure of molecules complicates magneto-optical trapping.

29 ing with 1-nm accuracy (FIONA) and dual-beam optical trapping.

30 , as revealed by atomic force microscopy and optical trapping.

31 ng interferometric scattering microscopy and optical trapping.

32 ing dual-labeled gliding filament assays and optical trapping.

33 apillary electrophoresis, patch-clamping and optical trapping.

34 ingle-molecule mechanical events examined by optical trapping.

35 n, such as rotor bead tracking(1-3), angular optical trapping(4) and magnetic tweezers(5), have helpe

36 ivated by attaching the motor to beads in an optical trap, a situation that may mimic attachment to I

37 ar system that creates a reliable and mobile optical trap, AFMOTs can find potential applications ran

38 The freely movable optical trap allows particles to be trapped in their nat

39 Single-molecule optical trapping allows ClpXP unfolding to be directly v

40 aman scattering from a single vesicle, while optical trapping allows more than hour-long observations

41 Optical trapping allows noninvasive probing of piconewto

42 It was previously shown that optical trapping allows the manipulation of micrometer-s

43 x-ray scattering (SAXS) and single-molecule optical-trap analyses are consistent with the high bendi

44 Using an optical trap and a two-channel flow cell to move single

45 n progressively through the transition by an optical trap and an algorithm is used to extract the ene

46 force required to hold the bacterium in the optical trap and determine the propulsion matrix, which

47 e this problem by alternately modulating the optical trap and excitation beams to prevent simultaneou

48 membranes of various cell types by using an optical trap and fast three-dimensional (3D) interferome

49 upper DPPC bilayer can be manipulated by the optical trap and the shape of the vesicle distorted from

50 r aqueous aerosol droplet is captured in the optical trap and used as a sampling volume, accreting ma

51 Here we employ both optical trapping and biochemical reconstitution with myo

52 nts of various lengths using single-molecule optical trapping and bulk fluorescence approaches in the

53 g cells, thereby extending cell viability in optical trapping and cell manipulation applications.

54 We show optical trapping and chemical identification of sub-20 m

55 Using an in vitro optical trapping and fluorescence assay, we found that K

56 Optical trapping and levitation also maintain optical al

57 t the single particle level using near-field optical trapping and light-scattering techniques.

58 Noncontact optical trapping and manipulation of micrometer- and nan

59 his paper describes a method, which combines optical trapping and microfluidic-based droplet generati

60 hat are arranged by electrodynamic (that is, optical trapping and optical binding) interactions.

61 ucidates the role of convection in plasmonic optical trapping and particle assembly, and opens up new

62 to optical polarization imaging, metrology, optical trapping and quantum information processing.

63 Here we demonstrate simultaneous optical trapping and rotation of a birefringent micropar

64 apable of simultaneous, spatially coincident optical trapping and single-molecule fluorescence.

65 We use single-molecule optical trapping and small-angle x-ray scattering, combi

66 We combine optical trapping and surface-enhanced Raman scattering t

67 Here, we employ simultaneous optical trapping and total internal reflection fluoresce

68 beyond what is ordinarily used in a magneto-optical trap, and being most sensitive to fields of the

69 integrin-molecule adhesion kinetics using an optical trap, and diffusion using fluorescence correlati

70 of the diffusion coefficient of a bead in an optical trap, and to demonstrate that it is not in gener

71 Here, we employ an optical-trap- and total internal reflection fluorescence

72 An ultrastable optical trapping apparatus capable of base pair resoluti

73 Here, we used an automated optical trapping apparatus in conjunction with a novel m

74 transcription elongation complexes, using an optical trapping apparatus that allows for the detection

75 Here, by combining ultrastable optical trapping apparatus with a novel two-bead assay t

76 Using a custom-modified optical trapping apparatus, we used a tightly focused in

77 chniques currently available, those based on optical trapping are promising.

78 The tight focus excitation requirements of optical trapping are well suited to confocal Raman micro

79 been an experimental challenge, as standard optical traps are too weak.

80 f tension applied with a feedback-controlled optical trap as the MTs lengthened approximately 1 micro

81 A molecules have previously been observed in optical traps as sudden changes in molecular extension.

82 rm the reader about recent progress in axial optical trapping, as well as the potential for these dev

83 Using a single-molecule optical trap assay, we found that vinculin forms a force

84 ion, applied load, and temperature, using an optical trapping assay capable of distinguishing pauses

85 We employ an optical trapping assay to investigate the behaviors of t

86 We employed an optical trapping assay to probe the motions of individua

87 ium of a DNA hairpin using a single-molecule optical trapping assay, in which the unfolded state is c

88 Using a unique real-time angular optical trapping assay, we found that RNAP working again

89 Using a three-bead optical trapping assay, we recorded NMIIB interactions w

90 With the use of an optical-trapping assay based on in situ transcription by

91 sphate (ATP) (2-4 muM) was measured using an optical-trapping assay featuring 1 base-pair (bp) precis

92 We developed an optical-trapping assay to follow the cotranscriptional f

93 We employed an ultrastable optical-trapping assay to follow the motion of individua

94 Here, we developed an optical-trapping assay to monitor the translocation of i

95 Here, we describe a single-molecule optical-trapping assay to study transcription initiation

96 Here, we used an optical-trapping assay with high spatiotemporal resoluti

97 recision to the widely used, surface-coupled optical-trapping assay.

98 duced force output and inability to stall in optical trap assays but exhibited increased speeds, run

99 the bead rotational fluctuations inherent in optical trapping assays where beads are used to apply th

100 The most commonly used optical-trapping assays are coupled to surfaces, yet suc

101 Such enhanced optical-trapping assays are revealing the fundamental st

102 Using an optical trap-based assay, we showed that the minimal cad

103 O) at the single-molecule level, we utilized optical trap-based force spectroscopy to measure the str

104 Using site-directed mutagenesis, optical trap-based force spectroscopy, and molecular mod

105 Here we used an optical trap-based system to measure the binding of sing

106 nally constrained DNA when measured using an optical-trap-based DNA-overstretching assay.

107 Employing an optical-trap-based electronic force clamp, we studied th

108 ity of myosin to produce force using a novel optical-trap-based isometric force in vitro motility ass

109 es, labeling with beads remains critical for optical-trapping-based investigations of molecular motor

110 ich are highly sought after in the fields of optical trapping, biological sensing and quantum informa

111 ing in the well-controlled environment of an optical trap but also for spores germinating when adhere

112 s from such cells by pulling on them with an optical trap but failed, even when we used forces large

113 using multifrequency excitation and in situ optical trap calibration.

114 dular optical tweezers (AFMOTs), in which an optical trap can be reliably created and freely moved on

115 Here we show that an infrared single-beam optical trap can be used to individually trap, transfer

116 We show that optical trapping can efficiently deform cell-cell interf

117 While optical traps can manipulate objects in three dimensions

118 rpin, recorded under constant force using an optical trap, can be used to reconstruct the energy land

119 We found that, on manipulation with an optical trap, cilia deflect by bending along their lengt

120 We demonstrate that optical trapping combined with confocal Raman spectrosco

121 Optical-trapping confocal Raman microscopy is developed

122 ly 0.6 microm in size) have been acquired by optical-trapping confocal Raman microscopy over the 900-

123 work with laser tweezers has suggested that optical traps could be used to create novel spatial prob

124 by changing the types of fibers for both the optical trap creation and particle position detection.

125 we develop an in silico model, supported by optical trapping data, suggesting that the motors' diffu

126 Here, we report a new class of on-chip optical trapping devices.

127 sed on scanning force microscopy imaging and optical trap-driven unzipping assays, it has recently be

128 at probe the H-NS-DNA interaction: a dynamic optical-trap-driven unzipping assay and an equilibrium H

129 When moving against load applied by an optical trap, dynein can decrease step size to 8 nm and

130 An ultra-high-speed optical trap enabled direct observation of the timing an

131 The optical trapping enables capturing of individual bacteri

132 s interaction with the anisotropic fluid and optical trap environment.

133 orough characterization of cell viability in optical trapping environments was performed.

134 resonance energy transfer (smFRET), using an optical trap equipped for simultaneous smFRET.

135 Recent optical trap experiments have applied resisting, assisti

136 Specifically, optical trap experiments revealed that force promotes a

137 Optical trapping experiments indicate that dynein bound

138 Optical trapping experiments reveal details of molecular

139 to perform high-precision and high-bandwidth optical trapping experiments to study motor regulation i

140 Optical trapping experiments were used to measure direct

141 n be used in both 3D confocal microscopy and optical trapping experiments while carefully tuning the

142 ) = 6.2 s(-1)) determined in single molecule optical trapping experiments, indicating that myosin 15-

143 Yet, as in previous optical trapping experiments, the forces imposed on carg

144 In optical trapping experiments, we found that increasing t

145 Single-molecule optical-trapping experiments are now resolving the small

146 gyroscopic directional stabilization and the optical trapping field.

147 Using an optical trap for spatial and temporal control over targe

148 Using optical trap force spectroscopy, we investigated the res

149 d concentrations and different directions of optical trap forces.

150 ams in these fluids can generate anisotropic optical trapping forces, even for particles larger than

151 rties, and the organelles manipulated by the optical trap frequently vary in size and shape.

152 Here by using a DNA-tethered optical trapping geometry, we find that the force-genera

153 tal advances are complemented by insights in optical-trapping geometry and single-molecule motility a

154 3 muK in a micro-fabricated grating magneto-optical trap (GMOT), enabling future mass-deployment in

155 Optical trapping has been implemented in many areas of p

156 While optical trapping has been the most explored method of le

157 The application of optical traps has come to the fore in the last three dec

158 pplying a slow triangle-wave movement to the optical traps holding a bead-actin-bead dumbbell.

159 e bio-chemical detection, reflective filter, optical trapping, hot-electron generation, and heat-assi

160 Optical trapping immobilizes the particle while maintain

161 hods that provide in situ calibration of the optical trap in the complex cellular environment, taking

162 We describe the exciting advances of using optical trapping in the field of analytical biotechnolog

163 hough our magnetic moulds currently resemble optical traps in that they are limited to the manipulati

164 molecular oxygen is generated locally by the optical traps in the presence of a sensitizer, which we

165 to be realized in miniature systems, but the optical traps in these systems lack reliability or mobil

166 al calibrations, results in a more versatile optical trapping instrument that is accurately calibrate

167 Using a high-resolution optical trapping instrument, we directly observed the pr

168 Miniaturizing optical trap instruments onto optofluidic platforms hold

169 ipulation of live cells in a dual-beam fibre-optical trap integrated into a modular lab-on-chip syste

170 stretching force is applied with a dual-beam optical trapping interferometer.

171 ines a time-shared ultrahigh-resolution dual optical trap interlaced with a confocal fluorescence mic

172 The force-field generated by a near-field optical trap is analyzed.

173 to apply forces on single molecules with an optical trap is combined with the endogenous structural

174 A type of dark-spot optical trap is devised that can cool large numbers of a

175 nanoparticle in the vicinity of a near-field optical trap is modeled using the Fokker-Planck equation

176 Optical trapping is a powerful manipulation and measurem

177 Plasmon-enhanced optical trapping is being actively studied to provide ef

178 technique in which cellular indentation via optical trapping is performed on cells at a high spatial

179 Optical trapping is potentially a powerful technique in

180 e of processive myosin motors as measured by optical trapping is similarly uncertain.

181 ended between two beads and held in separate optical traps is brought close to a surface that is spar

182 n array of stable, three-dimensional on-chip optical traps is formed at the antinodes of a standing-w

183 At low optical trap loads, we observed staircase-like processiv

184 Our cooling technique, in combination with optical trap manipulation, may enable otherwise unachiev

185 These results indicate that typical optical trap measurements of kinetics reflect the dynami

186 This method was applied to optical trap measurements of power strokes of the Drosop

187 Optical trap measurements revealed that the heterodimer

188 iate states was compared to the results from optical trapping measurements on the same dimer to disce

189 lecule solution kinetics and single-molecule optical trapping measurements provided in-depth insights

190 We propose and demonstrate a new optical trapping method for single cells that utilizes m

191 Using a modified optical-trapping method, we examined the group function

192 We report on optical trapping methodology capable of making precise i

193 in molecules in real time using magnetic and optical trapping micromanipulation techniques.

194 alize a two-stage, hexagonal pyramid magneto-optical trap (MOT) with strontium, and demonstrate loadi

195 er cooled atoms can be produced in a magneto-optical trap (MOT), in the absence of other vacuum pumpi

196 echnique in cold-atom physics is the magneto-optical trap (MOT), which combines laser cooling with a

197 from the vapour can be captured in a magneto-optical trap (MOT).

198 We tested this proposal by comparing optical trapping motility measurements of cover strand m

199 the main advantage of acoustic trapping over optical trapping, namely the ability of sound to propaga

200 Here, we use optical-trapping nanometry to probe the mechanics of enz

201 ated polystyrene beads, which are held in an optical trap near the cell membrane of a macrophage.

202 ere we demonstrate three-dimensional magneto-optical trapping of a diatomic molecule, strontium monof

203 by facilitating stable atmospheric-pressure optical trapping of individual particles and spectroscop

204 The energy and forces involved in optical trapping of lipid vesicles were derived in terms

205 Optical trapping of liposomes is a useful tool for manip

206 Successful optical trapping of magnetic beads was found to be depen

207 Magneto-optical trapping of molecules could provide a similarly

208 The results are consistent with the optical trapping of particles at or near the excitation

209 arming motility assays, video microscopy and optical trapping of single cells.

210 Optical trapping of single molecules in three-bead assay

211 emonstrate a quantum interface that combines optical trapping of solids with cavity-mediated light-ma

212 The next generation of single-beam optical traps offers revolutionary new opportunities for

213 ic device may find potential applications in optical trapping, optical data storage and many other re

214 Optical traps or "tweezers" use high-power, near-infrare

215 e found applications in material processing, optical trapping, or cell microscopy.

216 Optical catapulting (OC) and optical trapping (OT) have recently been combined with l

217 mic-force microscopy, magnetic tweezers, and optical traps (OTs) have been employed to probe the many

218 Here we demonstrate an indirect optical trapping platform which circumvents these limita

219 ic field enhancement in the plasmon-assisted optical trapping process.

220 ited for characterizing and aligning magneto-optical traps, requiring little or no additional equipme

221 Optical trapping results demonstrated that S217A does no

222 xciton confinement layer, and a conventional optical trapping scheme, we show a peak external quantum

223 plets for high-resolution measurements in an optical trap showed that they compare well with plastic

224 Single-molecule analysis of myosin by optical trapping showed a comparable 2-fold reduction in

225 include enabling high fractional filling of optical trap sites within PCWs, calibration of optical f

226 The model reproduces key signatures found in optical trapping studies of structurally defined complex

227 s work, we improved the spatial precision of optical trapping studies of transcription to approximate

228 e probability of multiple-motor transport in optical trapping studies.

229 A new optical trapping study shows that the stepsize of cytopl

230 We present a single-molecule optical-trapping study of the interactions of RNAPII wit

231 Using a dual optical trap system, we observed tether formation betwee

232 e present the development of an ultra-stable optical trapping system with angstrom-level resolution,

233 accuracy is crucial for force calibration of optical trapping systems.

234 In this study, we established the optical trapping technique for determining membrane mech

235 A novel optical trapping technique is described that combines an

236 Here, we use our recently developed optical trapping technique to characterize the swimming

237 e under tension and torque using the angular optical trapping technique.

238 Here we employ optical trapping techniques to investigate the structure

239 anscription elongation using single-molecule optical trapping techniques.

240 Using single-molecule optical-trapping techniques, we examined the force-induc

241 Using single molecule optical-trapping techniques, we found that betaCM has a

242 education, as well as helping transition the optical trapping technology from the research lab to the

243 We built an optical trap that can be calibrated in vivo during data

244 g pathways under the low-force regime of the optical trap; the specific unfolding pathway depends on

245 to freely control the number and position of optical traps, thus facilitating the unrestricted manipu

246 old-coated liposomes are maneuvered using an optical trap to achieve precise delivery of encapsulated

247 We used an optical trap to deflect bullfrog hair bundles and to mea

248 use measurements of swimming bacteria in an optical trap to determine fundamental properties of bact

249 We have used an optical trap to directly measure the forces generated by

250 We used an optical trap to directly probe the molecular determinant

251 l properties of a primary cilium by using an optical trap to induce resonant oscillation of the struc

252 We further use the optical trap to measure in vivo the detachment rates fro

253 Here, we used an optical trap to measure the bending rigidity of live Esc

254 We utilized an optical trap to measure the mechanical force to rupture

255 stigate these questions, we used a precision optical trap to measure the single-molecule kinetics of

256 The methodology uses the optical trap to probe force-free association of individu

257 We used a feedback-controlled optical trap to probe the velocity, run length, and unbi

258 Here, we use an optical trap to quantify motion of polystyrene beads dri

259 Here, we use single-molecule optical trapping to determine the mechanochemistry of tw

260 Here, we used optical trapping to determine the unitary and ensemble f

261 ngle-molecule gold nanoparticle tracking and optical trapping to examine the mechanism of coordinatio

262 ce (flow cell) used in conjunction with dual optical trapping to manipulate DNA dumbbells and to visu

263 Using dual optical trapping to manipulate DNA, and single-molecule

264 erties of microtubule cross-links we applied optical trapping to mitotic asters that form in mammalia

265 owever, questions remain over the ability of optical trapping to position objects for X-ray diffracti

266 d confocal Raman microscope is combined with optical trapping to probe and analyze bacterial spores i

267 Here we use single-molecule imaging and optical trapping to show that Lis1 does not directly alt

268 We used high-resolution optical trapping to study individual RecBCD molecules mo

269 Optical fiber-based trapping systems allow optical traps to be realized in miniature systems, but t

270 w vancomycin affects cell stiffness, we used optical traps to bend unflagellated mutants of B. burgdo

271 Here, we demonstrate the use of optical traps to manipulate, align, and assemble metal-s

272 hoice of wavelength and polarization for the optical trap, two electronic states of an atom can exper

273 cal and ensemble-level experiments involving optical trapping using a photonic force microscope and p

274 ith a fluorophore at high efficiency, and 3) optical trapping virometry to measure the number of gp12

275 A two-beam optical trap was used to measure the bending stiffness o

276 ally, by measuring the cargo dynamics in the optical trap, we find that there is memory: it is more l

277 ules held under tension in a high-resolution optical trap, we found that the native folding pathway i

278 of nitrogen-vacancy centers suspended in the optical trap, we observe distinct peaks in the measured

279 Using a high-precision optical trap, we show that an individual monomer of PCDH

280 With the optical trap, we stretched VWF multimers and a poly-prot

281 Using an improved application of an optical trap, we were now able to demonstrate that cytop

282 Using optical trapping, we observed myosin VI stepping against

283 ther, by integrating these enhancements with optical traps, we demonstrate how efficient bioconjugati

284 Using optical traps, we determine physicochemical triggering t

285 toms confined in an array of two-dimensional optical traps; we studied the spin-orbital quantum dynam

286 ingle living cells and beads suspended in an optical trap were recorded with 30-ms time resolution.

287 A classic example of this is an optical trap, which can hold a particle in a tightly foc

288 living microalgal cell held in place with an optical trap while simultaneously collecting Raman data.

289 e living bacterial cells held in place by an optical trap while simultaneously collecting Raman spect

290 d droplets (LDs) in COS1 cells respond to an optical trap with a remarkable enhancement in sustained

291 low laser power by combining a standing-wave optical trap with confocal Raman spectroscopy.

292 n of the biopolymer's elasticity by using an optical trap with nanometer-scale position resolution.

293 Combining optical trapping with hard X-ray microscopy techniques,

294 Combined optical trapping with single-molecule fluorescence imagi

295 Here, we combine optical trapping with TIRF-based microscopy to measure t

296 Finally, we demonstrate an array of magneto-optical traps with a single laser beam, which will be ut

297 we show that the spatial resolution of dual optical traps with dual-trap detection is always superio

298 usand times more atoms than previous magneto-optical traps with microfabricated optics and, for the f

299 ayer from the same vesicle is drawn into the optical trap, with an energy of approximately 6 x 10(-13

300 By setting up a well-calibrated single-beam optical trap within a fluorescence microscope system, on