ライフサイエンスコーパス: optogenetic

1 us), P2X(2) (chemogenetics), and CsChrimson (optogenetics).

2 nic and electronic circuitry with those from optogenetics.

3 abodies with the spatiotemporal precision of optogenetics.

4 ut surgery, enabling implant-free deep brain optogenetics.

5 n taste-mediated physiology and behavior via optogenetics.

6 egulatory elements and the conditionality of optogenetics.

7 two decades have witnessed the emergence of optogenetics; a field that has given researchers the abi

8 Optogenetic activation and inactivation of STN-projectin

9 N 5-HT neurons, cerebral 5-HT depletion, and optogenetic activation and silencing were performed.

10 Drosophila melanogaster central brain whose optogenetic activation increased, and genetic inactivati

11 cus on mechanosensory bristle neurons, whose optogenetic activation induces a similar sequence.

12 Pairing optogenetic activation of a single glomerulus with foot

13 Here, we show that optogenetic activation of AON neurons in awake male and

14 Here, we use optogenetic activation of beta-catenin signaling to prob

15 Optogenetic activation of CeA(GA) potently suppressed bo

16 nduced cortical gamma oscillations by either optogenetic activation of cortical parvalbumin-positive

17 mics this SSRI-induced hypolocomotion, while optogenetic activation of dopamine neurons reverses this

18 Single-unit MTC recordings revealed that optogenetic activation of glutamatergic AON terminals in

19 Here, we tested whether optogenetic activation of glutamatergic projections from

20 aumatic stress disorder, in combination with optogenetic activation of hypothalamic melanin-concentra

21 is circuit bidirectionally controls feeding: optogenetic activation of NAc projections to the VTA inh

22 We tested this idea using unilateral optogenetic activation of neurons in the dorsal striatum

23 Similar to optogenetic activation of PeF, panic-prone rats also exh

24 Optogenetic activation of PNOC(ARC) neurons in the ARC a

25 consisting of a lick behavior in response to optogenetic activation of predominantly nociceptive peri

26 Here we show that optogenetic activation of PV+ neurons in the basal foreb

27 causal significance, we found that rhythmic optogenetic activation of retrosplenial cortex layer 5 n

28 to drive behaviour with targeted two-photon optogenetic activation of small ensembles of L2/3 pyrami

29 Here we found that in vivo optogenetic activation of the basolateral amygdala-nucle

30 In contrast, optogenetic activation of the dFB promotes sustained wak

31 We find that optogenetic activation of the dopaminergic neuron DAN-i1

32 ial cells and nerve fibers and studies using optogenetic activation of the epithelium showed initiati

33 s are strongly inhibited by GABA(B)Rs, while optogenetic activation of the interneurons shows that th

34 Optogenetic activation of these neurons, referred to her

35 Despite loss of the DA signal, optogenetic activation of VTA glutamate cell bodies or a

36 Optogenetic activation or inhibition of the responsible

37 With genetic silencing and optogenetic activation, we show that LC11 is necessary f

38 esis recapitulates the therapeutic effect of optogenetic activation.

39 an have a strong influence on the effects of optogenetic activation/inhibition and must be considered

40 Two-photon activation of optogenetic actuators and calcium (Ca(2+)) imaging with

41 dual brain microvessels, calcium imaging and optogenetics allow the investigation of pericyte and smo

42 Optogenetics allows light-driven, non-contact control of

43 Here, we combined structural, functional and optogenetic analyses of the mouse retina to discover tha

44 dopamine receptors and received support from optogenetic and chemogenetic studies, it has been rarely

45 animal models, facilitated in large part by optogenetic and chemogenetic techniques.

46 Recently developed optogenetic and chemogenetic tools that allow high-fidel

47 Translational studies in rodents have used optogenetic and chemogenetic tools to identify not just

48 We combined optogenetic and electrophysiological approaches to inves

49 Using optogenetic and pharmacological manipulations in male mi

50 Here we use optogenetics and a combination of viral vectors in adult

51 (2020) use optogenetics and biophysical modeling to demonstrate how

52 e also show the superior capacity of BPI for optogenetics and calcium imaging of human neurons.

53 Using a combination of optogenetics and chemogenetics we show the involvement o

54 rst-order) and pulvinar (higher-order) using optogenetics and extracellular electrophysiology in awak

55 Here, optogenetics and functional mapping reveal cholinergic n

56 these tools (including microbial opsins for optogenetics and genetically encoded Ca(2+) indicators)

57 Using optogenetics and pharmacogenetics in combination with in

58 our automated training system with wireless optogenetics and pharmacology experiments, expanding the

59 Among others, promising approaches include optogenetics and prosthetic implants, which aim to bypas

60 Using behavioral, electrophysiological, optogenetic, and biochemical tools in adult male Wistar

61 Utilizing genetics, optogenetics, and calcium imaging, we identify a new rol

62 ted channelrhodopsins known, long-sought for optogenetics, and more broadly the most red-shifted micr

63 h embryos together with subcellular imaging, optogenetics, and photopharmacology, we show that, in vi

64 euronal activity by peripheral inflammation, optogenetics, and selective proteasome inhibition of dop

65 Combined electrophysiology, optogenetics, and statistical analysis suggested that S-

66 Cellular optogenetics applications remain limited with diffusible

67 Here, we report an optogenetic approach that utilizes an engineered Light-R

68 ributions affect cellular functions, several optogenetic approaches enable organelle repositioning th

69 Optogenetic approaches for controlling Ca(2+) channels p

70 Here, by using optogenetic approaches in mice (both males and females),

71 ers of either population, for example, using optogenetic approaches or photoablation, has confirmed t

72 Here we develop different optogenetic approaches that use light to activate TrkB s

73 lls in adult mice using paired recording and optogenetic approaches.

74 Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca(2+) permea

75 Optogenetic-assisted circuit mapping revealed functional

76 Optogenetic-assisted circuit mapping reveals a preferent

77 Optogenetics-assisted circuit mapping in brain slices re

78 l processes and are promising candidates for optogenetics because of their modular nature and long-ra

79 Here, using in vivo electrophysiology, optogenetics, behavioral tasks and mathematical modeling

80 Here, we present an ultra-light-sensitive optogenetic Ca(2+) modulator, named monSTIM1 encompassin

81 Thus, optogenetics can be used to achieve quantitative and tem

82 of FLiCRE's modular design, we expressed an optogenetic channel selectively in this cell type and sh

83 We used optogenetic channelrhodopsin (ChR2) excitations at the s

84 ns are created in laboratory rats by pairing optogenetic channelrhodopsin (ChR2) stimulation of centr

85 the integration of new technologies, such as optogenetics, chemogenetics, and calcium imaging, manipu

86 in the brain, and enable the application of optogenetics, chemogenetics, calcium imaging and related

87 Anatomic and optogenetic circuit mapping indicated that MSDB GABA neu

88 inding a lack of local connectivity, we used optogenetic circuit mapping to study the strength of LH

89 Optogenetics combines optical and molecular biology (gen

90 We test this prediction using an optogenetic competition assay whereby two targeted light

91 The first hypothesis is supported by our optogenetic competition experiments: iterative spatial c

92 ns are increasingly being adopted for use in optogenetic constructs.

93 We established rapid and reversible optogenetic control for Set2, the sole H3K36 methyltrans

94 neuron activity and muscle contraction under optogenetic control for the study of normal physiologica

95 nstrate exactly this capability by combining optogenetic control of Ras/extracellular signal-related

96 to placing a wide variety of proteins under optogenetic control with Z-lock.

97 iplex protein regulation via dual-wavelength optogenetic control.

98 aevis embryos when using such a conventional optogenetic design for the fibroblast growth factor rece

99 Prolonged optogenetic dFB activation nevertheless achieved a key s

100 ecially appealing candidates for control via optogenetic dimerization methods.

101 We find using the CIBN-CRY2 optogenetic dimerization system that the phosphoryl grou

102 We find that optogenetic disruption of this MN activity profoundly im

103 Our optogenetic engineering approach can be broadly applied

104 Furthermore, we provide optogenetic evidence for an inhibitory GABAergic Agrp->o

105 Intriguingly, both optogenetic excitation and inhibition of cerebellar cort

106 In a response-based task, brief optogenetic excitation at the onset of runs decreased ru

107 On-demand optogenetic excitation of glutamatergic fastigial neuron

108 Intriguingly, optogenetic excitation of these AT1aR neurons of the lam

109 Optogenetic excitation of these neurons shows a linear r

110 Optogenetic excitation robustly elevated blood pressure,

111 ach combining cell-type-specific anterograde optogenetic excitation with single-cell recordings targe

112 Same-site NAc optogenetic excitations also attenuated recruitment of F

113 titive or defensive motivations, using local optogenetic excitations to oppose putative DNQX-induced

114 Optogenetic experiments are challenging because CsChrims

115 in Drosophila, introducing chemogenetic and optogenetic experiments for the first time.

116 its, which can be distinguished by simulated optogenetic experiments.

117 Optogenetic genome engineering tools enable spatiotempor

118 Optogenetics has revolutionized neuroscience in small la

119 Optogenetics has revolutionized the neurosciences, incre

120 Using cell class-specific optogenetic identification and inhibition during cortica

121 We used calcium imaging and optogenetics in a sequential decision task for mice to s

122 Using optogenetics in Caenorhabditis elegans, we solved the pr

123 In vivo recording combined with optogenetics in mice revealed that these two populations

124 empts, successful and unsuccessful, of using optogenetics in the primate brain.

125 Using optogenetics in unanesthetized rats, we found that selec

126 By using a combination of optogenetics, in vivo electrophysiology, and machine lea

127 Optogenetic inactivation of LO dramatically disrupted ch

128 Indeed, we observed that optogenetic inactivation of PV neurons during one tone c

129 ar expression in LB mice was rescued through optogenetic inactivation of PV-positive cells in the BLA

130 Optogenetic inactivation showed that the somatosensory c

131 tion imaging of projection neurons and focal optogenetic inactivation, we studied mice performing a w

132 rospects for clinical translation of cardiac optogenetics, including new optical therapies for rhythm

133 his is reversed by extinction training or by optogenetic induction of in vivo long-term depression (L

134 Using a combination of optogenetic inhibition and excitation in vivo and in sit

135 Both epidermal cell optogenetic inhibition and interruption of ATP-P2X4 sign

136 Conversely, tonic optogenetic inhibition of BF-PV neurons partially rescue

137 of PV GPe neuron activity was ameliorated by optogenetic inhibition of coincident D2-SPN activity, (3

138 Optogenetic inhibition of DA neurons in either region sl

139 On-demand optogenetic inhibition of glutamatergic neurons in the f

140 arning was unaffected by iSPN manipulations, optogenetic inhibition of iSPNs, but not dSPNs, impaired

141 ceptors inhibits DMV cholinergic neurons and optogenetic inhibition of liver-projecting parasympathet

142 Pharmaco- and optogenetic inhibition of MEC led to a disruption of bor

143 Conversely, optogenetic inhibition of OVLT neurons attenuated thirst

144 t to fastigial neurons is inhibitory, direct optogenetic inhibition of the fastigial nucleus had no e

145 hyperactive, despite being disinhibited, (5) optogenetic inhibition of the STN exacerbated abnormal G

146 eking and food intake (in both sexes), while optogenetic inhibition of this circuit potentiates food-

147 y of visual input during motor training, and optogenetic inhibition of visual cortex activity impairs

148 Next, we used optogenetic inhibition of VTA TH neurons to show distinc

149 Optogenetic inhibition paused force generation or produc

150 sure, water intake, and sodium intake, while optogenetic inhibition produced the opposite effects.

151 Here, we combined this approach with in vivo optogenetic inhibition to identify and interrupt the act

152 Reversible NAcc dysfunction, via optogenetic inhibition, specifically impaired rapid disc

153 pression of inhibitory firing in response to optogenetic inhibitory stimulation.

154 Furthermore, optogenetic interrogations demonstrate that these neuron

155 Optogenetics is among the most widely employed technique

156 Optogenetics is the genetic approach for controlling cel

157 However, optogenetics lags in plant research because ambient ligh

158 to a stressor and the anxiogenic effects of optogenetic LC activation.

159 Here we report that tonic optogenetic manipulation of an ascending arousal system

160 Applying 2-photon calcium imaging and optogenetic manipulation of anatomically defined PNs, we

161 ng with high-resolution behavioral tracking, optogenetic manipulation of individually identified neur

162 ordings, in vivo single-unit recordings, and optogenetic manipulation of OVLT neurons, was used in ad

163 Es predicted changes in task engagement, and optogenetic manipulation of the VP during reward deliver

164 n, was not observed, and the efficacy of the optogenetic manipulation on behavior was maintained acro

165 Using behavioral tests in combination with optogenetic manipulation, we show that, in contrast to w

166 ions by parametrically varying the timing of optogenetic manipulations in a Pavlovian conditioning ta

167 of stress hormones, immediately followed by optogenetic manipulations of either the avBST or its pro

168 Using activity-dependent optogenetic manipulations of natively active ensembles,

169 Importantly, optogenetic manipulations revealed that while activation

170 Bidirectional optogenetic manipulations were targeted to the principal

171 hysiological recordings, pharmacological and optogenetic manipulations, and an ad hoc behavioral prot

172 Using multielectrode recording and optogenetic manipulations, we show that VIP neurons prov

173 To develop a minimally invasive optogenetic method that overcomes this challenge, we eng

174 We demonstrate an optogenetic method to control gene expression and metabo

175 dal-interneuron connections triggered by the optogenetic modification of the firing rate and spike sy

176 We measured the effects of selective optogenetic modulation of cortico-collicular feedback pr

177 therapies for seizure control, and on-demand optogenetic modulation of the cerebellar cortex was show

178 these effects are observed in two different optogenetic mouse lines.

179 rt a neurotechnology for selective epineural optogenetic neuromodulation of nociceptors and demonstra

180 Techniques such as optogenetics or sensory stimulation appear to engage can

181 Using a dexterity task, calcium imaging, optogenetic perturbations, and behavioral manipulations,

182 Optogenetic perturbations, live imaging, and time-resolv

183 Optogenetic photosilencing was employed to assess the fu

184 ther generalized this strategy by developing optogenetic platforms to control three neurotrophic trop

185 Here, using an optogenetic probe that rapidly and robustly activates Rh

186 ement unit and an LED driver for an external optogenetic probe.

187 ral progenitor cell differentiation using an optogenetic RAF1 system (OptoRAF1).

188 We found that optogenetic reactivation of these socially active neuron

189 Optogenetic rescue was possible even using a simple, all

190 circuit mapping to study the strength of LH optogenetic responses and network oscillations, which we

191 Optogenetic responses are recorded more than one year fo

192 sent electrophysiological, chemogenetic, and optogenetic results demonstrating the key role of GC in

193 We envision that these ligand-independent optogenetic RTKs will provide useful toolsets for the de

194 ither genetically directed deletion or acute optogenetic silencing of DR(Sert) neurons dramatically i

195 REM sleep-specific optogenetic silencing of LH(vgat) cells induced a reorga

196 Optogenetic silencing of this sparse ABN activity during

197 Optogenetic silencing of ventral hippocampal (VH) termin

198 In our laboratory, optogenetic stimulation after trauma exposure was suffic

199 We therefore combined cerebellar optogenetic stimulation and CA1 calcium imaging with an

200 sequence of impaired motor function, because optogenetic stimulation did not alter motor performance.

201 These results demonstrate that optogenetic stimulation driven coherent neuronal dynamic

202 Our results demonstrate that optogenetic stimulation during neural differentiation ca

203 ne how coherent neuronal dynamics arise from optogenetic stimulation in the primate brain.

204 An optogenetic stimulation mimicking this LC-CA1 activity i

205 Remarkably, repeated M2-DLS optogenetic stimulation normalized motor behavior in HD

206 Further, within the PVN, selective optogenetic stimulation of afferents that arise from the

207 ly, working memory deficits were restored by optogenetic stimulation of astrocytes with melanopsin.

208 Constant, low wattage optogenetic stimulation of basal forebrain (BF) neurons

209 Prior evidence indicates that optogenetic stimulation of BLA projections to the medial

210 It involves optogenetic stimulation of cells stably transfected to e

211 cits a flavor aversive effect, and selective optogenetic stimulation of ChR2-expressing SF1 projectio

212 Here we show that optogenetic stimulation of cortical glutamatergic affere

213 assembly and display calcium activity after optogenetic stimulation of cortical neurons.

214 Glutamate uncaging or optogenetic stimulation of cortical spheroids triggers r

215 We investigated the effects of optogenetic stimulation of D1+ (direct) and A2A+ (indire

216 We used optogenetic stimulation of either glutamatergic or choli

217 Optogenetic stimulation of GAD65(+) TBCs increased chord

218 Optogenetic stimulation of GAD65(+) TBCs on the anterior

219 onditions to assess the behavioral impact of optogenetic stimulation of GAD65(+) TBCs.

220 the first definitive evidence that selective optogenetic stimulation of glial-like GAD65(+) TBCs evok

221 tions of SNr neurons, which we confirm using optogenetic stimulation of GPe terminals within the SNr.

222 Moreover, optogenetic stimulation of graft-derived axons extending

223 Optogenetic stimulation of LN-innervating sensory fibers

224 Chemogenetic or optogenetic stimulation of mPFC->pPVT neurons in adultho

225 Intersectional optogenetic stimulation of MPOA neurons that express ESR

226 Reality (PiVR) system to conduct closed-loop optogenetic stimulation of neural functions in unrestrai

227 forated patch-clamp recording, we found that optogenetic stimulation of nigrostriatal dopamine axons

228 We also demonstrate that chemogenetic or optogenetic stimulation of npvf-expressing neurons induc

229 y, and latency) using holographic two-photon optogenetic stimulation of olfactory bulb neurons with c

230 cked endogenous oscillatory activity through optogenetic stimulation of parvalbumin-expressing intern

231 In this study, we show that in vivo optogenetic stimulation of prelimbic (PrL) and infralimb

232 Optogenetic stimulation of projections from these neuron

233 arkinson's disease, where cell type-specific optogenetic stimulation of PV(+) neurons over other neur

234 Finally, closed-loop optogenetic stimulation of SOM, but not PV, terminated s

235 Direct optogenetic stimulation of STN neurons was effective in

236 motility patterns produced by electrical and optogenetic stimulation of sympathetic pathways.

237 in the cortex in response to repeated brief optogenetic stimulation of thalamocortical afferents.

238 Postspatial training optogenetic stimulation of the BLA-mEC pathway altered t

239 In vitro optogenetic stimulation of the claustrum induced excitat

240 aventricular thalamus (PVT), selective chemo/optogenetic stimulation of the PVT-projecting SF1 neuron

241 Optogenetic stimulation of this liver-projecting melanoc

242 Optogenetic stimulation or inhibition of bursty subicula

243 Optogenetic stimulation promises to provide new ways to

244 The reduction in MTC firing during optogenetic stimulation was confirmed in recordings in a

245 effects of STN DBS using cell type-specific optogenetic stimulation with a much faster opsin, Chrono

246 ns have a strong influence on the effects of optogenetic stimulation.SIGNIFICANCE STATEMENT Whether S

247 The wireless optogenetics stimulation in the subcutaneous adipose tis

248 ition controls spike timing, suggesting that optogenetic-stimulation-driven coherence may arise from

249 issue by using extracellular recordings and optogenetic stimulations in mice across postnatal develo

250 Optogenetic STN DBS at 130 pulses per second (pps) reduc

251 High-rate optogenetic STN DBS can indeed ameliorate parkinsonian m

252 ified the behavioral and neuronal effects of optogenetic STN DBS in female rats following unilateral

253 stepping similarly to electrical DBS, while optogenetic STN DBS with ChR2 did not produce behavioral

254 The optogenetic strategies presented are promising tools to

255 Using chemogenetic and optogenetic strategies, we show that these neurons and t

256 Using an optogenetic strategy combined with in vivo electrophysio

257 This optogenetic strategy highlights the impact of Nlgn1 intr

258 Additionally, genetic and optogenetic studies demonstrate that CCAP signaling is n

259 Optogenetic studies in vivo and dynamic clamp studies in

260 Optogenetic studies support a causal relationship betwee

261 tral to vision and emerging opportunities in optogenetics, super-resolution microscopy, and photoacti

262 This chemo-optogenetic system will be particularly applicable in sy

263 we present a near-infrared light-activatable optogenetic system, which combines the specificity and o

264 lm formation, automated in silico control of optogenetic systems, and readout of multiple orthogonal

265 Here, we utilized optogenetic systems, optoRaf and optoAKT, to delineate t

266 RNA regulator system will be widely used for optogenetics, targeted cell ablation, subcellular manipu

267 We conclude that non-neuronal optogenetics targets damaged neurons and signaling subci

268 Optogenetic techniques for neural inactivation are valua

269 BF parvalbumin (PV) neurons in arousal using optogenetic techniques in mice.

270 Recent optogenetics techniques have enabled patterned perturbat

271 scribe strategies for single- and two-photon optogenetics that allow manipulation of the activity of

272 as mutagenesis, chemical modifications, and optogenetics that have been used to re-engineer existing

273 To make evidence-based decisions in primate optogenetics, the scientific community would benefit fro

274 Using perforated patch recordings and optogenetics, they show that dopamine release persistent

275 bition and must be considered when employing optogenetic to study high-rate neural stimulation.

276 anslational opportunities and challenges for optogenetics to be fully embraced in cardiology are also

277 To test this, we used optogenetics to stimulate VTA glutamate neurons in which

278 Here we use slice physiology and optogenetics to study vHPC-evoked feed-forward inhibitio

279 (NAMs), transgenic mice, and viral-assisted optogenetics to test the hypothesis that selective inhib

280 uctance, making it an ideal target for chemo-optogenetic tool development.

281 ant usable light-switch elements (PULSE), an optogenetic tool for reversibly controlling gene express

282 evance of GluD2 ion channel and introduce an optogenetic tool that will provide a novel and powerful

283 tics in vivo, we used a rapid and reversible optogenetic tool, the light-inducible nuclear exporter,

284 tral cross talk with visible light excitable optogenetic tools and fluorescent probes, and decreased

285 A growing number of optogenetic tools have been developed to reversibly cont

286 Optogenetic tools have revolutionized the study of recep

287 ribe a nanobody-based targeting approach for optogenetic tools in mammalian cells and in vivo in zebr

288 ck sufficient spatiotemporal accuracy, while optogenetic tools suffer from non-physiological response

289 abodies should make their incorporation into optogenetic tools the versatile technology to regulate e

290 use optogenetic tools to investigate whether the splenic imm

291 driving the use of causal manipulations with optogenetic tools to study higher cognitive functions in

292 Capitalizing on these optogenetic tools, we identify the molecular determinant

293 with the use of green fluorescent probes and optogenetic tools.

294 Here, we combine fiber photometry, chemo/optogenetics, virus-assisted retrograde tracing, ChR2-as

295 -clamp electrophysiology, and input-specific optogenetics, we employed a targeted pharmacological app

296 Using in vitro electrophysiology and optogenetics, we examined how Delta(9)-THC alters cortic

297 ing behavior, in vivo electrophysiology, and optogenetics, we first show that the primary visual cort

298 al tracing, single-cell transcriptomics, and optogenetics, we identified and functionally tested a se

299 Combining optogenetics with nanobody-based targeting will pave the

300 propose an experimental scheme that combines optogenetics with single-vesicle membrane fusion, aiming