ライフサイエンスコーパス: optogenetics

1 cally targetable optical control of neurons (optogenetics).

2 lar functions with light sensitive proteins (Optogenetics).

3 us), P2X(2) (chemogenetics), and CsChrimson (optogenetics).

4 used to control neuronal firing with light (optogenetics).

5 ibition using in vitro electrophysiology and optogenetics.

6 ategies in electrotherapy, gene therapy, and optogenetics.

7 se as biological imaging, photovoltaics, and optogenetics.

8 possible by combining stem cell therapy and optogenetics.

9 ses and are attractive candidates for use in optogenetics.

10 on; that is, this opsin enables transcranial optogenetics.

11 GABA(A) alpha2 receptor knockout mouse with optogenetics.

12 an be used as efficient inhibitory tools for optogenetics.

13 to label D2R+ neurons for calcium imaging or optogenetics.

14 ch-clamp recordings with calcium imaging and optogenetics.

15 a novel tool for flexible, high-conductance optogenetics.

16 netics: a non-invasive, US-based analogue of optogenetics.

17 V1 activation pattern as the one elicited by optogenetics.

18 may be important for CrChR2 applications in optogenetics.

19 ich make them efficient inhibitory tools for optogenetics.

20 ion channels with extensive applications in optogenetics.

21 opy, optical coherence tomography (OCT), and optogenetics.

22 of the alteration of synaptic physiology by optogenetics.

23 ular pathways is an important application of optogenetics.

24 c LOV-based photosensors with application in optogenetics.

25 logy, various neural imaging modalities, and optogenetics.

26 nic and electronic circuitry with those from optogenetics.

27 abodies with the spatiotemporal precision of optogenetics.

28 ut surgery, enabling implant-free deep brain optogenetics.

29 n taste-mediated physiology and behavior via optogenetics.

30 egulatory elements and the conditionality of optogenetics.

31 solar energy harvesting, photochemistry, and optogenetics.

32 elds of photobiology, energy conversion, and optogenetics.

33 Optogenetics, a widely used technique in neuroscience re

34 two decades have witnessed the emergence of optogenetics; a field that has given researchers the abi

35 dual brain microvessels, calcium imaging and optogenetics allow the investigation of pericyte and smo

36 The use of optogenetics allowed us to vary the dynamics and probabi

37 Optogenetics allows for phototactic guidance, steering,

38 Optogenetics allows light-driven, non-contact control of

39 fundamental question by taking advantage of optogenetics, anatomical, and electrophysiology approach

40 between layer 5 pyramidal cells by combining optogenetics and 2-photon calcium imaging in mouse neoco

41 Here we use optogenetics and a combination of viral vectors in adult

42 Here we use optogenetics and a computational simulation to determine

43 , ultrastructural analysis, calcium imaging, optogenetics and behavioral analyses, we uncovered a cir

44 (2020) use optogenetics and biophysical modeling to demonstrate how

45 e also show the superior capacity of BPI for optogenetics and calcium imaging of human neurons.

46 We use cell-type-specific optogenetics and chemogenetics (DREADDs) to modulate act

47 Using a combination of optogenetics and chemogenetics we show the involvement o

48 fundamental question by taking advantage of optogenetics and directly examining the functional effec

49 lving spatially biasing actin nucleation via optogenetics and disruption of mitochondrial distributio

50 Here we compare cell-targeted optogenetics and electrical microstimulation in the maca

51 rst-order) and pulvinar (higher-order) using optogenetics and extracellular electrophysiology in awak

52 Here we combine optogenetics and fMRI to produce a brain-wide 5-HT evoke

53 develop in vivo optical sensors, such as for optogenetics and force transduction.

54 Here, optogenetics and functional mapping reveal cholinergic n

55 these tools (including microbial opsins for optogenetics and genetically encoded Ca(2+) indicators)

56 Using in vivo optogenetics and high-speed Ca(2+) imaging, we show that

57 sing a novel "opto-dialysis" probe to couple optogenetics and in vivo microdialysis, we report that o

58 sms by stimulating directly IT neurons using optogenetics and measured the effect of photo-stimulatio

59 Electrophysiological recordings, optogenetics and molecular biology experiments were perf

60 e dynamics of acetylcholine release, we used optogenetics and paired recordings from CHIs and medium

61 Using optogenetics and pharmacogenetics in combination with in

62 Here we use in vivo calcium imaging, optogenetics and pharmacological approaches to show that

63 our automated training system with wireless optogenetics and pharmacology experiments, expanding the

64 imic the tremorgenic action of the drug with optogenetics and present evidence that highly patterned

65 Among others, promising approaches include optogenetics and prosthetic implants, which aim to bypas

66 application of these photosensing systems in optogenetics and synthetic biology.

67 e use anatomical tracing, electrophysiology, optogenetics, and 2-photon Ca(2+) imaging to determine h

68 f nociception, using novel transgenic lines, optogenetics, and calcium imaging in behaving larval zeb

69 Utilizing genetics, optogenetics, and calcium imaging, we identify a new rol

70 nipulating circuit function using mutations, optogenetics, and drugs.

71 omputational simulation, two-photon imaging, optogenetics, and dual-color uncaging of glutamate and G

72 circuit tracing, morphology, chemogenetics, optogenetics, and electrophysiology to obtain a more com

73 and in vivo electrophysiological recordings, optogenetics, and fiber-photometry-based calcium imaging

74 We used electrophysiology, optogenetics, and fluorescent in situ hybridization to c

75 genetics, Airyscan microscopy, live imaging, optogenetics, and Forster resonance energy transfer to p

76 Using a combination of pharmacology, optogenetics, and linear regression methods, we estimate

77 microscopy, electrophysiology, biochemistry, optogenetics, and molecular biology, have dramatically i

78 ted channelrhodopsins known, long-sought for optogenetics, and more broadly the most red-shifted micr

79 ection of cardiomyocyte subpopulations using optogenetics, and opens new frontiers of exploration int

80 Using transgenic mice, optogenetics, and pharmacogenetics, we studied the role

81 P-16.48 Using a combination of pharmacology, optogenetics, and phenotypic analyses we determine that

82 h embryos together with subcellular imaging, optogenetics, and photopharmacology, we show that, in vi

83 euronal activity by peripheral inflammation, optogenetics, and selective proteasome inhibition of dop

84 Combined electrophysiology, optogenetics, and statistical analysis suggested that S-

85 iod activity modulation via odorant stimuli, optogenetics, and transgenic tetanus toxin neurotransmis

86 Thus, ChR2 optogenetics appears well suited to noninvasively expose

87 Cellular optogenetics applications remain limited with diffusible

88 friendly technology with broad potential for optogenetics applications.

89 in vivo using a combined viral-infection and optogenetics approach to drive expression of channelrhod

90 Here we use the optogenetics approach to selectively stimulate neurons i

91 atomical, electrophysiological, imaging, and optogenetics approaches have established that the audito

92 lectrical microstimulation and more recently optogenetics are widely used to map large-scale brain ci

93 s fields such as in vivo optical imaging and optogenetics, are spearheading their popularity in biolo

94 Moreover, VirChR1s have unique potential for optogenetics as they lack possibly noxious Ca(2+) permea

95 Optogenetics-assisted circuit mapping in brain slices re

96 R", is particularly promising for inhibitory optogenetics because of its combination of larger curren

97 l processes and are promising candidates for optogenetics because of their modular nature and long-ra

98 Recent studies combining optogenetics, behavioral assays, neural tracing, and neu

99 Here, using in vivo electrophysiology, optogenetics, behavioral tasks and mathematical modeling

100 Thus, optogenetics can be used to achieve quantitative and tem

101 of the LGN konio neurons with CamK-specific optogenetics caused selective electrical current inflow

102 Using optogenetics, cell-specific ablation, whole cell patch-c

103 the integration of new technologies, such as optogenetics, chemogenetics, and calcium imaging, manipu

104 in the brain, and enable the application of optogenetics, chemogenetics, calcium imaging and related

105 Optogenetics combines optical and molecular biology (gen

106 e, we present a suite of technologies to use optogenetics effectively in primates and apply these too

107 Furthermore, combining immunohistochemistry, optogenetics, electrophysiology, and fast-scan cyclic vo

108 Moreover, two-photon optogenetics enable the possibility of artificially impr

109 Alternatively, a sister approach, synthetic optogenetics, enables photocontrol over proteins of mamm

110 We then describe how to perform a typical optogenetics experiment using the optoPlate-96 to stimul

111 This workflow thus allows complex optogenetics experiments (independent control of stimula

112 , a platform for high-throughput three-color optogenetics experiments that allows simultaneous manipu

113 tuning can further broaden their utility in optogenetics experiments.

114 Thus, synthetic optogenetics facilitates interrogation of native neurona

115 Finally, we employed CaMPARI and optogenetics for functional circuit mapping in ex vivo a

116 brane potential with light is fundamental to optogenetics for research and clinical applications.

117 Despite common use of ChR2 in optogenetics for selective control and monitoring of ind

118 conclude with a discussion of the utility of optogenetics for treating sensorimotor hearing loss and

119 By adapting optogenetics for use in non-neural cells in embryos, we

120 Here, we use pharmacology, optogenetics, genetics, and electrophysiology to investi

121 ever, outside of neuroscience, the impact of optogenetics has been limited by a lack of user-friendly

122 r, the application of allosteric switches in optogenetics has been scarce and suffers from critical l

123 Optogenetics has been successfully implemented in the he

124 KEY POINTS: Optogenetics has emerged as a potential alternative to e

125 ABSTRACT: Optogenetics has emerged as a potential alternative to e

126 TATEMENT Recent developments in the field of optogenetics has enabled researchers to probe the neuron

127 Chemo-optogenetics has produced powerful tools for optical con

128 Optogenetics has revolutionized neuroscience in small la

129 Optogenetics has revolutionized the neurosciences, incre

130 rial version phycocyanobilin, often used for optogenetics, has a dramatically stabilized Pfr state.

131 vent of powerful perturbation tools, such as optogenetics, has created new frontiers for probing caus

132 Recent advances in optogenetics have enabled simultaneous optical perturbat

133 Recent advances in optogenetics have opened new routes to drug discovery, p

134 ce in-situ hybridization, microfluidics, and optogenetics, have opened the door to a large number of

135 Here, we use intersectional genetics, optogenetics, high-throughput behavioral analysis, singl

136 nding questions in primate neuroscience, but optogenetics holds the promise to overcome this hurdle.

137 t only demonstrate, for the first time using optogenetics, how the spinal modules follow linearity in

138 We used calcium imaging and optogenetics in a sequential decision task for mice to s

139 in the SNc controls mouse behavior, we used optogenetics in awake behaving mice and found that activ

140 Recent advances in the use of optogenetics in awake behaving rodents has added an addi

141 Using optogenetics in Caenorhabditis elegans, we solved the pr

142 rich virtual reality set-ups and the use of optogenetics in freely moving animals.

143 probes for advanced in vivo pharmacology and optogenetics in freely moving rodents.This protocol is a

144 le-cell patch-clamp recordings combined with optogenetics in male and female transgenic mice, we show

145 address this issue, we used activity-guided optogenetics in male Sprague Dawley rats to silence IL p

146 in vitro and in vivo electrophysiology with optogenetics in mice and found the following: (1) the IG

147 In vivo recording combined with optogenetics in mice revealed that these two populations

148 Using optogenetics in mice, we show that orbitofrontal and ant

149 Using DREADDs and optogenetics in mice, we show that the output of the bas

150 ied by in-vivo ECoG recordings combined with optogenetics in mice.

151 Using in vitro electrophysiology and optogenetics in mouse brain slices, we found that ACh ge

152 Using two-photon holographic optogenetics in mouse primary visual cortex, we tested w

153 Applications of optogenetics in multicellular organisms, however, have n

154 ound that selective stimulation of SChIs via optogenetics in normal mice robustly and reversibly ampl

155 is question using patch-clamp recordings and optogenetics in olfactory bulb slices from transgenic mi

156 However, implementing optogenetics in plants has been less straightforward, gi

157 emented to overcome the challenges for using optogenetics in plants.

158 We used trans-synaptic viral tracing, optogenetics in slice preparations, and bouton size anal

159 re we combine two-photon calcium imaging and optogenetics in tethered flying flies with circuit model

160 Here, we used optogenetics in Th::Cre rats to selectively stimulate VT

161 bining quantitative behavioral analysis with optogenetics in the head-fixed setup, we established a n

162 empts, successful and unsuccessful, of using optogenetics in the primate brain.

163 Using optogenetics in unanesthetized rats, we found that selec

164 e identified with single unit recordings and optogenetics in vivo.

165 By using a combination of optogenetics, in vivo electrophysiology, and machine lea

166 Optogenetics, in vivo ganglion imaging, and genetically

167 rospects for clinical translation of cardiac optogenetics, including new optical therapies for rhythm

168 iguration of cortical circuits by two-photon optogenetics into neuronal ensembles that can perform pa

169 Optogenetics is a powerful research approach that allows

170 Optogenetics is a revolutionary tool to assess the roles

171 Optogenetics is among the most widely employed technique

172 Optogenetics is an emerging biotechnology armed with the

173 Optogenetics is an optical technique that exploits visib

174 Cell assemblies manipulation by optogenetics is pivotal to advance neuroscience and neur

175 utilizing upconversion-nanoparticle-mediated optogenetics is presented.

176 A key assumption of optogenetics is that light only affects opsin-expressing

177 Optogenetics is the genetic approach for controlling cel

178 While the usefulness of optogenetics is unquestionable, we argue that this metho

179 Despite recent advances in optogenetics, it remains challenging to manipulate gene

180 Using optogenetics, it was confirmed that basal forebrain affe

181 With the rise of ChR2 use in optogenetics, it will be critical to identify residues t

182 However, optogenetics lags in plant research because ambient ligh

183 ce that the described mitochondrial-targeted optogenetics may have a broad application for studying t

184 eyond 700 nm would generate new prospects in optogenetics, membrane sensor technology, and complement

185 tes at multiple sites, coupled to the use of optogenetics, not to globally turn on or off neurons of

186 t that repetitive activation with two-photon optogenetics of neuronal populations from ensembles in t

187 While optogenetics offers great potential for linking brain fu

188 Techniques such as optogenetics or sensory stimulation appear to engage can

189 w the progress that has been made in primate optogenetics over the past 5 years.

190 We used chemogenetics, optogenetics, pharmacology, and a translationally analog

191 Specifically, an integrative approach of optogenetics, pharmacology, electrophysiology, and behav

192 pconversion applications, in particular, for optogenetics, photodynamic therapy, and photochemistry.

193 custom tracking, selective illumination, and optogenetics platform to compare two mechanosensory syst

194 Optogenetics promises precise spatiotemporal control of

195 Optogenetics promises to deepen our understanding of how

196 tion of glioma membranes assessed by in vivo optogenetics promotes proliferation, whereas pharmacolog

197 -vessel fMRI method and its combination with optogenetics provide a platform for mapping the hemodyna

198 In vivo optogenetics provides unique, powerful capabilities in t

199 In optogenetics, researchers use light and genetically enco

200 e frequency of slow waves for one month with optogenetics resulted in increased amyloid beta - depend

201 nd blockage of pericytes in combination with optogenetics, reveal that pericyte blockage facilitates

202 Channelrhodopsin-based optogenetics shows the feasibility of stimulating neural

203 20 marks a decade of developments in cardiac optogenetics since this technology was adopted from neur

204 The wireless optogenetics stimulation in the subcutaneous adipose tis

205 tral to vision and emerging opportunities in optogenetics, super-resolution microscopy, and photoacti

206 RNA regulator system will be widely used for optogenetics, targeted cell ablation, subcellular manipu

207 We conclude that non-neuronal optogenetics targets damaged neurons and signaling subci

208 Recent optogenetics techniques have enabled patterned perturbat

209 scribe strategies for single- and two-photon optogenetics that allow manipulation of the activity of

210 as mutagenesis, chemical modifications, and optogenetics that have been used to re-engineer existing

211 Using in vivo optogenetics, the brain region-specific inputs to the NA

212 eation of new proteins for illuminating, via optogenetics, the fundamentals of brain function.

213 In combination with stimulation using optogenetics, the reporters revealed changes in neuronal

214 To make evidence-based decisions in primate optogenetics, the scientific community would benefit fro

215 Using perforated patch recordings and optogenetics, they show that dopamine release persistent

216 Using a combination of in vitro and in vivo optogenetics, this work demonstrates that interglomerula

217 KR2 is a promising tool for optogenetics, thus directed engineering to modify ion se

218 Here we used optogenetics to activate or inhibit mouse STN to test it

219 affect the local circuits, we use two-photon optogenetics to activate them individually in mouse visu

220 Using optogenetics to augment dopamine concentration, we found

221 anslational opportunities and challenges for optogenetics to be fully embraced in cardiology are also

222 Here, we use optogenetics to characterize responses to pulsatile RhoA

223 Using optogenetics to control both the location and the timing

224 ion-specific circuit mechanisms, we employed optogenetics to control mesopontine cholinergic neurons

225 ion of whole-cell patch-clamp recordings and optogenetics to demonstrate that ethanol potently depres

226 We used optogenetics to demonstrate that the pedunculopontine te

227 use genetics, electrophysiology, imaging and optogenetics to directly target major classes of spinal

228 We used optogenetics to drive individual mouse CA1 hippocampal n

229 Here, we used optogenetics to examine the involvement of parvalbumin-e

230 Here we combine targeted recordings and optogenetics to examine the synaptic underpinnings of th

231 ns of ventral pallidal neurons, we next used optogenetics to examine whether changes in synaptic plas

232 ArchaerhodopsinT3.0 (ArchT) loss-of-function optogenetics to explore BP regulation by C1 neurons in i

233 ve circuit, we used AgRP-neuron ablation and optogenetics to explore connectivity in acute slice prep

234 used Archaerhodopsin-based loss-of-function optogenetics to explore the contribution of these neuron

235 se anterior cingulate cortex (ACC), by using optogenetics to induce oscillations in activity, can pro

236 A new study deploys optogenetics to induce the yeast bud on demand, at a sit

237 To this end, the current study used optogenetics to inhibit the BLA during specific task pha

238 dy combines neuronal ensemble recordings and optogenetics to map a functional gradient in rodent pref

239 y somatosensory cortex (S1) of mice by using optogenetics to map the connections between parvalbumin

240 While they are widely used in optogenetics to optically control neuronal activity, rho

241 egrating personalized immunoengineering with optogenetics to overcome critical hurdles in cancer immu

242 cium imaging in head-fixed flying flies with optogenetics to overwrite the existing population repres

243 Using inducible gene expression and optogenetics to perturb the network at different levels,

244 of T cell signaling and extend the reach of optogenetics to probe pathways where the individual mole

245 We used optogenetics to selectively activate the GABAergic nigro

246 ere we test this hypothesis in mice by using optogenetics to selectively reactivate neural ensembles

247 Electrophysiological assays using optogenetics to stimulate activated versus nonactivated

248 ascertain this underlying mechanism, we used optogenetics to stimulate cholinergic afferents in prefr

249 To test this, we used optogenetics to stimulate VTA glutamate neurons in which

250 the Cre-recombinase/loxP system in mice with optogenetics to structurally and functionally characteri

251 Here we use slice physiology and optogenetics to study vHPC-evoked feed-forward inhibitio

252 stigates short-term recognition memory using optogenetics to target glutamatergic neurons within the

253 in single-cell calcium imaging combined with optogenetics to test the capacity of PVs and CHIs to aff

254 We used cell-type-specific optogenetics to test the functional relationship between

255 (NAMs), transgenic mice, and viral-assisted optogenetics to test the hypothesis that selective inhib

256 oduces the precise spatiotemporal control of optogenetics to the molecular control of synaptic functi

257 tion discrimination task in mice while using optogenetics to transiently silence adult-born neurons a

258 rimary visual cortex (V1), we use two-photon optogenetics to trigger action potentials in a targeted

259 Here we apply optogenetics to understand how subpopulations of beta-ce

260 the observed behaviors, and used functional optogenetics to validate selected model predictions.

261 on two-photon calcium imaging and two-photon optogenetics, to detect, characterize, and manipulate ne

262 It has generated excitement as an optogenetics tool for the manipulation of cyclic nucleot

263 illardia theta is a potent neuron-inhibiting optogenetics tool.

264 Here, we expand the optogenetics toolbox in the form of a tunable, high-cond

265 aging technologies, fluorescent sensors, and optogenetics tools for cell biology are advancing.

266 Through the use of optogenetics, viral tracing, and electrophysiological re

267 Here, we combine fiber photometry, chemo/optogenetics, virus-assisted retrograde tracing, ChR2-as

268 ere, using mathematical modeling and in vivo optogenetics we reveal for the first time how this neura

269 vivo electrophysiology, calcium imaging, and optogenetics, we demonstrate a novel function of VTA(Vga

270 Using optogenetics, we demonstrate that activation of 5-HT ter

271 Using optogenetics, we demonstrate that adult-born granule cel

272 -clamp electrophysiology, and input-specific optogenetics, we employed a targeted pharmacological app

273 Using in vitro electrophysiology and optogenetics, we examined how Delta(9)-THC alters cortic

274 Using optogenetics, we examined the role of the basal ganglia

275 ing behavior, in vivo electrophysiology, and optogenetics, we first show that the primary visual cort

276 By harnessing the temporal specificity of optogenetics, we found that FEF contributes to memory-gu

277 al tracing, single-cell transcriptomics, and optogenetics, we identified and functionally tested a se

278 us two-photon calcium imaging and two-photon optogenetics, we identified and selectively activated pl

279 Using optogenetics, we probe yeast polarization and find that

280 Using optogenetics, we show that modulation of firing rates by

281 atic reactions, bioorthogonal chemistry, and optogenetics, we tether fluorescent proteins, model enzy

282 , by combining in vivo neural recordings and optogenetics, we unexpectedly find that both suppressing

283 ly useful in biomedical applications such as optogenetics where a light source is used to trigger a c

284 ve used a brain stimulation technique called optogenetics, which allowed targeting a specific type of

285 Optogenetics, which uses visible light to control the ce

286 chronized Kiss1(ARH) neuronal activity using optogenetics, whole-cell electrophysiology, molecular ph

287 Combining optogenetics with a closed-loop stimulation approach in

288 We combined optogenetics with calcium imaging and pharmacology to de

289 Here, we combined optogenetics with electrophysiology to characterize the

290 We detail how to combine 2P-DH and synthetic optogenetics with electrophysiology, or with red fluores

291 Using optogenetics with extracellular and whole-cell electroph

292 the conventional "outside-in" approaches of optogenetics with fiber implantation, our method provide

293 voltammetry, electrophysiology, and in vivo optogenetics with localized pharmacology to identify neu

294 Combining optogenetics with nanobody-based targeting will pave the

295 tridges for chronic in vivo pharmacology and optogenetics with selective manipulation of brain circui

296 propose an experimental scheme that combines optogenetics with single-vesicle membrane fusion, aiming

297 ermed three-dimensional scanless holographic optogenetics with temporal focusing (3D-SHOT), which all

298 By combining optogenetics with voltage-sensing microelectrodes, we de

299 To facilitate expansive optogenetics without the need for invasive implants, our

300 o generate localized photon flux in vivo for optogenetics would greatly benefit from such an approach