ライフサイエンスコーパス: organotypic

1 Organotypic 3D culture of fresh tumor tissue enables con

2 Here, using organotypic 3D cultures, we found that primary human mes

3 he integration and live behavior of pMGLs in organotypic 3D cultures.

4 Consistently, human organotypic 3D skin models using stable STRA6KD HaCaT ce

5 We recently described a novel organotypic 3xTg-AD mouse brain slice culture model with

6 hymal interactions using a three-dimensional organotypic air-liquid interface coculture system.

7 Organotypic airway cultures pinpoint TAp73 as necessary

8 ired benzodiazepine resistance, using rodent organotypic and acute brain slices.

9 Novel cellular, three-dimensional organotypic, and ex vivo models are considered and the c

10 This is supported by an organotypic assay showing that Mtss1-expressing cells di

11 ponse to HGF both in 2D and 3D physiomimetic organotypic assays; which can be suppressed by inhibitio

12 he invasion of glioblastoma cells through an organotypic brain slice and glioma progression in vivo.

13 Organotypic brain slice culture models provide an altern

14 Using organotypic brain slice cultures generated from embryoni

15 ng cultured cell lines, primary neurons, and organotypic brain slices from an AD mouse model, we foun

16 in the neocortical neural stem cell niche in organotypic brain slices from embryonic mice.

17 an HD mouse striatal cell model and HD mouse organotypic brain slices we found that D(1)R-induced cel

18 been demonstrated in cultured neurons and in organotypic brain slices, but not in acute brain slices

19 cells, as well as in synthetic HA matrix and organotypic brain slices.

20 ]i in individual astrocytes was performed in organotypic brainstem slice cultures and acute brainstem

21 to the aromatase inhibitor anastrazole in an organotypic breast model.

22 volatile-mediated communication between the organotypic bronchiole and cultures of Aspergillus fumig

23 haracterize the inflammatory response of the organotypic bronchiole to infection.

24 To complement direct infection of the organotypic bronchiole, we present a clickable extension

25 s this challenge, researchers have developed organotypic cancer models (organoids) that combine the 3

26 A 3-dimensional organotypic cell culture model of esophageal maturation

27 In this study, we established organotypic cell cultures of AT explants to study the im

28 Using chemical proteomics and an organotypic cell-based drug screening assay, we determin

29 Depleting DOCK10 in organotypic cerebellar cultures resulted in dramatic den

30 ctive in promoting precocious myelination in organotypic cerebellar slice cultures, and in vivo in ea

31 after lysolecithin-induced demyelination of organotypic cerebellar slice cultures.

32 was found to be disturbed, and treatment of organotypic cerebellar slices with ceramide significantl

33 Three-dimensional organotypic co-culture assays revealed that WNT2-mediate

34 ce for our screening-ready three-dimensional organotypic co-culture platform.

35 Organotypic co-cultures bridge the gap between standard

36 Organotypic co-cultures represent an easy, affordable an

37 , SSc-FBs, partially inhibit angiogenesis in organotypic coculture assays, and this is reversed by tr

38 compartments, we adapted a three-dimensional organotypic coculture model, originally developed to pro

39 rray of Wnt ligands in the three-dimensional organotypic coculture revealed increased mesenchymal exp

40 ammatory exposures in a dorsal root ganglion organotypic coculture system.

41 eroxia models were used: A three-dimensional organotypic coculture using primary human lung cells, pr

42 deling of primary airway epithelial cells in organotypic coculture with mast cells, we show that epit

43 ore invasive when grown in three-dimensional organotypic collagen gel cultures.

44 Here we describe the engineering of an organotypic colon cancer model by recellularization of a

45 human bronchial epithelial cells cultured in organotypic conditions closely approximated gene express

46 Organotypic cortical brain slices exposed to ischemic in

47 We used organotypic cortical cultures from rat brains as a biolo

48 Similarly, in organotypic corticoamygdalar slice cultures from immatur

49 l epithelial cells and the application of 3D organotypic culture (OTC), a form of tissue engineering.

50 ering RNA (siRNA)-mediated knockdown in skin organotypic culture and functional assessment of barrier

51 Organotypic culture and human tumour explants were allow

52 We then developed a cochlear organotypic culture and video-microscopy approach to mon

53 We show that SCN slices in organotypic culture demonstrate rapid and sustained circ

54 ells by either methylcellulose suspension or organotypic culture induces LMP1 expression prior to Z a

55 siRNA-knockdown of EMSY in organotypic culture leads to enhanced development of bar

56 e observations illustrate the utility of the organotypic culture model for the investigation of intra

57 ection of S631G variant into a 3-dimensional organotypic culture model of normal esophageal squamous

58 The rat hippocampal organotypic culture model of post-traumatic epilepsy was

59 This 3D organotypic culture model reproduced key features of des

60 re mutant (Kv2.1W365C/Y380T: Kv2.1 DN) in an organotypic culture model to manipulate channel expressi

61 A unique three-dimensional (3D) organotypic culture model was established; within a dilu

62 idering the physiological relevance of human organotypic culture models and their utility for high-th

63 have all led to valuable clinical insights, organotypic culture models have emerged as tractable sys

64 The alginate scaffold-based organotypic culture system is a promising, reliable, and

65 We used an organotypic culture system of human fetal testes explant

66 Analysis of spheroid invasion in an organotypic culture system revealed that these "trailbla

67 engineered an intestinal organotypic culture system that is capable of long-term

68 isms, we present a novel breathing brainstem organotypic culture that generates rhythmic neural netwo

69 d stabilized cysts in three-dimensional (3D) organotypic culture with E-cadherin fragment expression

70 nicotinic activation of alpha7-nAChRs in WT organotypic culture, as well as cell culture, increases

71 Here, using spatially defined organotypic culture, intravital microscopy of mammary tu

72 sponses as modeled in three-dimensional (3D) organotypic culture.

73 rized architecture in three dimensional (3D) organotypic culture.

74 at postnatal days (P7-P14) and maintained in organotypic culture.

75 e of Herpes simplex virus (HSVTK) cerebellar organotypic cultured slices markedly aggravated prion-in

76 analysis procedure for structurally complex organotypic cultures (CALYPSO) applied to fluorescence-b

77 5-positive tumor biopsy specimens (plugs) or organotypic cultures (rafts) consisting of ChHV5-positiv

78 ECs in rigid and compliant three-dimensional organotypic cultures and 2) restores the induction of th

79 is model for monitoring CMA in live animals, organotypic cultures and cell cultures from these mice,

80 use of biomechanically compliant or rigid 3D organotypic cultures and combined them with global micro

81 Furthermore, by using skin organotypic cultures as a model system to monitor the co

82 cellular P. gingivalis in both monolayer and organotypic cultures compared to free antibiotic or poly

83 In this study, we use organotypic cultures derived from transgenic mice induci

84 nd rescues gap junction coupling in cochlear organotypic cultures from connexin-deficient mice that a

85 in IPF fibroblasts and collagen secretion in organotypic cultures from IPF patients, thus suggesting

86 In the present study, we further exploit organotypic cultures from wild-type and SOD1(G93A) mice

87 Controlling the microenvironment of such organotypic cultures has impact in tissue engineering, c

88 potential decline and is rescued in cochlear organotypic cultures in low potassium milieu, indicating

89 agging of endogenous proteins in primary and organotypic cultures in vitro and developing, adult, age

90 elium exists as a stratified tissue in vivo, organotypic cultures may serve as a better model of EBV

91 NA-mediated knock down of Ano1 expression in organotypic cultures of Ano1 WT small intestine.

92 nisms, we used microscopy-based assays on 3D organotypic cultures of Caco-2 cysts as a model system.

93 In organotypic cultures of cerebellar slices, brain-derived

94 Finally, organotypic cultures of darkly pigmented human keratinoc

95 Using a combination of genetic approaches, organotypic cultures of embryonic pancreata, and genomic

96 e, we demonstrate that EBV is able to infect organotypic cultures of epithelial cells to establish a

97 rmaceutical agents and treatment regimens on organotypic cultures of human disease to expedite drug a

98 Using organotypic cultures of Madin-Darby canine kidney cells

99 Organotypic cultures of murine outer ear and vibrissal s

100 d Ca(2+) signaling in astrocyte processes in organotypic cultures of rat hippocampus.

101 Here, we show that when organotypic cultures of rodent hippocampal slices were t

102 rrier in Pelo-deficient mice, we made use of organotypic cultures of skin explants from control and m

103 enyl 153 (PCB153) on the proteome of primary organotypic cultures of the mouse mammary gland.

104 with morpholino antisense oligos in cochlear organotypic cultures revealed a negative correlation bet

105 ting the growth of breast cancer cells in 3D-organotypic cultures that recapitulated pulmonary microe

106 ive protocols for downstream applications of organotypic cultures that will allow users to (i) test t

107 this study, we used isogenic benign mammary organotypic cultures to interrogate the role of mTOR-med

108 , FN enhanced outgrowth in three-dimensional organotypic cultures via a mechanism that is dependent u

109 pression of differentiation markers in these organotypic cultures were analyzed by using immunohistol

110 Treatment of UVB-exposed monolayer or organotypic cultures with trichostatin A, a histone deac

111 alization of beta-catenin in vitro and in 3D organotypic cultures, accompanied by upregulation of WNT

112 tested in three-dimensional tracheospheres, organotypic cultures, air-liquid interface cultures, and

113 Here, by using NP cells, brain organotypic cultures, and in vivo animal models, we inve

114 orted long-term epidermal regeneration in 3D organotypic cultures, and resulted in the manifestation

115 ian cells lines, as well as mouse cerebellar organotypic cultures, and we also show that they exhibit

116 induced in primary astrocytes, neurons, and organotypic cultures, demonstrating that this phenomenon

117 In cell and organotypic cultures, Dkk4 inhibition is eliminated by e

118 solated cells of the ICC lineage and gastric organotypic cultures, hyperglycemia stimulated prolifera

119 In thymic organotypic cultures, the thymocyte-induced reduction in

120 inflammation in mouse primary astrocyte and organotypic cultures, through the AMPK signaling pathway

121 In organotypic cultures, UVB treatment also resulted in inc

122 anized knockin (KI) mouse model and in vitro organotypic cultures, we found that reoxygenation or cal

123 emistry in murine cells, tissues, or retinal organotypic cultures, we report that a naphthoquinone/qu

124 Using both mammary organoids and 3D organotypic cultures, we show that MMP activity is neces

125 In 3D organotypic cultures, we showed that a spatially distinc

126 rin beta1; and increased cell invasion in 3D organotypic cultures.

127 ammary epithelia in vivo, ex vivo, and in 3D organotypic cultures.

128 h two-colour Ca(2+) and glutamate imaging in organotypic cultures.

129 ld be unlike other herpesviruses examined in organotypic cultures.

130 opment and also upon stimulation in cortical organotypic cultures.

131 cer diagnosis was further demonstrated in an organotypic cutaneous squamous cell carcinomas model, al

132 t collagen IV production were examined in an organotypic dento-epithelial (OD-E) model.

133 oliferation, indicating that within the CNS, organotypic differences in EC metabolism exist.

134 regenerating tissues and acquire features of organotypic ECs.

135 thus creating an AP-1 gradient and enabling organotypic endothelial cell specialization of the vascu

136 nd screens to decipher the crosstalk between organotypic endothelial cells and parenchymal cells for

137 Uncovering the mechanisms by which organotypic endothelium distributes physiological levels

138 blished an in vitro model of rMS using mouse organotypic entorhino-hippocampal slice cultures.

139 icient to reduce terminal differentiation of organotypic epidermal skin equivalents, indicating overl

140 d a highly productive infection of HPV-18 in organotypic epithelial cultures.

141 helial barrier function, as documented in 3D organotypic epithelial cultures.

142 ed the gene signature, which was verified in organotypic ex vivo culture of patient-derived fibrotic

143 Ex vivo transduction of mouse organotypic explants identified Anc80L65 from a set of o

144 entify attributes that confer ECs with their organotypic features.

145 l programs generate cell diversity within an organotypic framework, enabling the later physiological

146 -derived cardiomyocytes and fibroblasts with organotypic functionality under serum-free conditions.

147 rt, and liver, among others, with increasing organotypic functionality.

148 and similarly decreasing endogenous TRIO in organotypic hippocampal brain slices significantly incre

149 onal networks were successfully grafted into organotypic hippocampal brain slices, showing an approxi

150 nt of epilepsy after brain injury, using the organotypic hippocampal culture model of post-traumatic

151 lusions of the study are as follows: (1) the organotypic hippocampal culture model of post-traumatic

152 in vitro, transfection of pyramidal cells in organotypic hippocampal cultures, and imaging of evoked

153 Using rat organotypic hippocampal cultures, we found that IL-1beta

154 the pattern of synaptic connectivity in the organotypic hippocampal cultures.SIGNIFICANCE STATEMENT

155 We induced tau hyperphosphorylation in organotypic hippocampal slice cultures (OHSC) and applie

156 mage stemming from ROS and RNS production in organotypic hippocampal slice cultures (OHSC), as well a

157 Extracellular fluid collected from rat organotypic hippocampal slice cultures (OHSCs) by electr

158 We have tested this by examining organotypic hippocampal slice cultures (OHSCs) exposed t

159 ethine and CoA in the extracellular space of organotypic hippocampal slice cultures (OHSCs).

160 metric or intensiometric Ca(2+) indicator in organotypic hippocampal slice cultures (one- and two-pho

161 dary assays (i.e. post-treatment protocol in organotypic hippocampal slice cultures and cortical neur

162 Using organotypic hippocampal slice cultures and primary neuro

163 Oxygen/glucose deprivation-induced injury in organotypic hippocampal slice cultures confirmed that ca

164 e of ASIC1a in AMPAR ischaemic plasticity in organotypic hippocampal slice cultures exposed to oxygen

165 ays can be regulated, in cortical neuron and organotypic hippocampal slice cultures from rat, either

166 mbrane in both primary neuronal cultures and organotypic hippocampal slice cultures from wild-type mi

167 We imaged organotypic hippocampal slice cultures of rat, in which

168 whole-cell recordings of pyramidal cells in organotypic hippocampal slice cultures that were generat

169 functional microglia-neuron interactions in organotypic hippocampal slice cultures, i.e., postnatal

170 ctions in vivo in EAE animals and ex vivo in organotypic hippocampal slice cultures.

171 In addition, acute treatment of an organotypic hippocampal slice epilepsy model with Sema4D

172 avior after deleting Pten from neurons in an organotypic hippocampal slice network.

173 use high-resolution two-photon microscopy in organotypic hippocampal slices from GAD65-GFP mice of bo

174 to mitochondrial and synaptic dysfunction in organotypic hippocampal slices of rats.

175 mia in vivo or oxygen-glucose deprivation in organotypic hippocampal slices or neurons, p75(NTR) is r

176 We show in rat organotypic hippocampal slices that prolonged network si

177 Using live-cell confocal microscopy of rat organotypic hippocampal slices, we find that enhancing n

178 Here, using mouse organotypic hippocampal slices, we show that the extrace

179 In addition, using organotypic human midgestation chorionic villous explant

180 ediated knockdown and phenotypic analysis in organotypic human tissue.

181 els in vivo and enhances leptin signaling in organotypic hypothalamic slices.

182 3D-organotypic invasion assays reveal that CCB02 has broad

183 ances in the design and assembly of in vitro organotypic liver and gastrointestinal (GI) models can a

184 Organotypic liver culture models for hepatotoxicity stud

185 ells, which do not vascularize tissues in an organotypic manner.

186 In this study, we used two-dimensional and organotypic melanoma culture models in combination with

187 f intercellular signalling networks requires organotypic microscale systems that facilitate the cultu

188 Here, we have engineered a unique organotypic model of angiogenic sprouting and neovessel

189 three-dimensional tissue culture to build an organotypic model of bronchial dysplasia.

190 on remyelination was verified in an ex vivo organotypic model of demyelination.

191 Here we use an engineered organotypic model of perfused microvessels to show that

192 Here we introduce a microscale organotypic model of the human bronchiole for studying p

193 An organotypic model of tumor-stroma interactions on a micr

194 veloped microfluidic device as a 3D in vitro organotypic model to mechanistically study tumor-stroma

195 ect comparison between these next generation organotypic models and more traditional methods, we obse

196 ensive and resource intensive, the design of organotypic models has renewed significance.

197 nd 3-dimensional cell culture models and new organotypic models that better replicate tissue structur

198 to inhibit local cancer cell invasion in 3D organotypic models.

199 , as important regulators of this process in organotypic models.

200 organoids derived from embryonic stem cells, organotypic mouse cerebellar slices, a xenotypic human-z

201 this study, using oral epithelial cultures, organotypic oral mucosal constructs, and a mouse model o

202 valis within monolayers of keratinocytes and organotypic oral mucosal models.

203 Here, we create three-dimensional organotypic pancreatic cultures, named pancreatoids, com

204 Thus, organotypic pancreatoids provide a useful tool for devel

205 mouse ES cells (ES-NS) and co-cultured with organotypic preparations of gut tissue consisting of the

206 HPV16-immortalized tonsillar cells grown in organotypic raft culture, we showed robust EBV replicati

207 Tumor cell invasion was studied using organotypic raft cultures and in vivo significance was a

208 Our deep-sequencing analysis of organotypic raft cultures identified microRNA 145 (miR-1

209 specifically regulated by HPV16 and HPV18 in organotypic raft cultures of foreskin and vaginal kerati

210 Using organotypic raft cultures of primary human keratinocytes

211 When grown as organotypic raft cultures, keratinocytes infected with w

212 l interactions in the HPV16 life cycle using organotypic (raft) cultures as a model.

213 ytes in monolayer cultures and their derived organotypic (raft) cultures, although it exhibits only a

214 ntiated into ESPCs and PSC-derived epidermal organotypic rafts (PSC-EORs).

215 In addition, HPV16-low folate-organotypic rafts contained fewer HPV16 viral particles,

216 host cell transcriptomes of natural lesions, organotypic rafts, and human papillomavirus (HPV)-immort

217 mic DNA when compared with HPV16-high folate-organotypic rafts.

218 g human (BC-1-Ep/SL) keratinocytes and HPV16-organotypic rafts.

219 infection of cervical, foreskin, and tonsil organotypic rafts.

220 er cells and glutamate-induced cell death in organotypic rat brain slices, suggesting similarities be

221 In organotypic rat hippocampal slices, we find that a nonph

222 Treatment of three-dimensional organotypic RDEB skin cultures with inhibitors of TGFbet

223 and mesenchymal stem cell-derived factors in organotypic retinal explant culture and an in vivo model

224 ofiles of specific BC types of mouse and rat organotypic retinal explants after 1 to 3 weeks in tissu

225 duced phase advances of PER2::LUC rhythms in organotypic SCN cultures from GIRK2 KO mice.

226 mCry1-luc organotypic SCN slices exhibited stable circadian biolum

227 tion, and differentiation gene expression in organotypic skin culture.

228 ocalized to the dermal-epidermal junction in organotypic skin culture.

229 entiation, and migration in large numbers of organotypic skin cultures comprising epidermis and dermi

230 We used human 3-dimensional organotypic skin cultures consisting of atopic fibroblas

231 lopment by using a genetic model of RDEB and organotypic skin cultures.

232 Cultured keratinocytes and organotypic skin equivalents depleted of Dsg1 exhibited

233 We develop a human organotypic skin melanoma culture (OMC) that allows real

234 es and in abnormal epidermal architecture in organotypic skin models recovering from UVB exposure.

235 histamine to human keratinocyte cultures and organotypic skin models reduced the expression of the di

236 Using organotypic skin models stimulated with Th2 cytokines IL

237 We used human 3-dimensional organotypic skin models with either primary keratinocyte

238 Using organotypic skin models with primary keratinocytes from

239 T cells elicited a differentiation defect in organotypic skin models, associated with reduced epiderm

240 Using 3-dimensional organotypic skin models, we show that NAM inhibits diffe

241 pression was determined in three-dimensional organotypic skin models.

242 ng bacterial growth on the three-dimensional organotypic skin models.

243 pigment to surrounding keratinocytes in a 3D organotypic skin reconstruct.

244 ative lipid analysis, electron microscopy of organotypic skin, and immunohistochemistry of human skin

245 function of BLA principal neurons in a novel organotypic slice culture (OTC) model of male rat BLA, a

246 sible mechanisms were analyzed both in mouse organotypic slice culture and in rat cell culture by ind

247 This hybrid Organotypic Slice Culture Assay coupled with RT-QuIC (OS

248 We recently developed an organotypic slice culture assay for sensitive detection

249 We used a novel organotypic slice culture model of BLA, complemented wit

250 screening system coupled to an ex vivo prion organotypic slice culture model to rapidly advance ratio

251 ta2*-nAChRs by nicotine either in vivo or in organotypic slice culture quickly elevates the number of

252 in a chemical-induced demyelination model on organotypic slice culture, in a BDNF-dependent manner.

253 s two-photon imaging of graded potentials in organotypic slice cultures and in Drosophila.

254 Spinal organotypic slice cultures from a mutant form of human s

255 s developed using z-stack images from murine organotypic slice cultures from central nervous system t

256 We employed hippocampal organotypic slice cultures from mice lacking key presyna

257 Using organotypic slice cultures from the forebrain and cerebe

258 We have used organotypic slice cultures from wild type and G93A SOD1

259 We show in vivo in rats and ex vivo in mouse organotypic slice cultures that posttraumatic GABA(A)-me

260 Circadian oscillation of mouse SCN organotypic slice cultures was monitored as PER2::LUC bi

261 Organotypic slice cultures were prepared from male and f

262 iated knockdown in rat and mouse hippocampal organotypic slice cultures, a postsynaptic density-95 (P

263 , we use advanced live imaging techniques of organotypic slice cultures, clonal analysis, and mathema

264 In organotypic slice cultures, repeated NPY treatment reduc

265 odotoxin or AP5 treatment in rat hippocampal organotypic slice cultures.

266 ppocampal cell death in mechanically injured organotypic slice cultures.

267 imited migration in a subset of human glioma organotypic slice cultures.

268 ngle-trial detection of action potentials in organotypic slice cultures.

269 We have developed an organotypic slice preparation of the normal portions of

270 nfocal time-lapse imaging in rat hippocampal organotypic slices and assessed the role of GluN3A-conta

271 noid 3 receptors (EP3R), breathing brainstem organotypic slices and optogenetic inhibition of EP3R(+/

272 of new therapies in human heart tissue using organotypic slices isolated from donor and end-stage fai

273 h3A interaction with interfering peptides in organotypic slices or in vivo induces a decrease of the

274 mpal neurons, dentate granule cells in mouse organotypic slices, and layer 2/3 pyramidal neuron in so

275 ical neurons, dentate granule cells in mouse organotypic slices, and layer 2/3 pyramidal neurons in t

276 In organotypic slices, NMDAR-dependent LTD of AMPAR excitat

277 In cultured neurons and hippocampal organotypic slices, synaptic release was reduced up to 1

278 KO hippocampal organotypic slices, which lack an intact peripheral immu

279 ing structural plasticity in rat hippocampal organotypic slices, which retain the diversity of neuron

280 sed motility and increased dissociation from organotypic spheroids.

281 on of ThT-negative, acylated SOD1 fibrils to organotypic spinal cord failed to produce the SOD1 inclu

282 onal outgrowth across the lesion gaps in the organotypic spinal cord slices, compared with controls.

283 In a previous study, we developed organotypic spinal cultures from an ALS mouse model expr

284 st decade, the generation and maintenance of organotypic structures has been propelled to the center

285 Cultivation of tumour slices required organotypic support materials and atmospheric oxygen for

286 Here, using an organotypic system that recapitulates distinct stages of

287 ct of deregulated SOX2 expression in a novel organotypic system that recreates the molecular and micr

288 pose a pathway to prevention that uses human organotypic systems that recapitulate hallmarks of aging

289 In organotypic three-dimensional cell cultures, endogenous

290 ethyl maleate-induced oxidative stress in an organotypic three-dimensional culture assay.

291 Using the mouse mammary gland, we designed organotypic three-dimensional culture models that permit

292 ere defined in monolayer and differentiated, organotypic three-dimensional cultures of rat epidermal

293 gulated alternative splicing (AS) targets in organotypic three-dimensional MCF-10A cell cultures that

294 In human organotypic three-dimensional skin and skin reconstituti

295 Three-dimensional organotypic tissue culture (OTC) is an innovative three-

296 ging of SP-transgenic granule cells in mouse organotypic tissue cultures we found that spines contain

297 en modest success in producing hiPSC-derived organotypic tissues or organoids.

298 arget proteins in confined intercellular and organotypic tissues, with reduced steric hindrance and n

299 ture of bone marrow-derived macrophages with organotypic tumour slices results in an upregulation of

300 ional mechanisms that drive angiogenesis and organotypic vascular endothelial cell specialization are