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1 64 h for 90Y-citrate and Te = 255 h for 32P-orthophosphate).
2 /- 0.03 mg-P.L(-1) (0.01 +/- 0.02 mg-P.L(-1) orthophosphate).
3 following incubation of cells with [(32)P(i)]orthophosphate.
4 h (15) N-labelled algal necromass and (33) P orthophosphate.
5 from MDA-486 cells labeled in vivo with [32P]orthophosphate.
6 sphate, releasing two molecules of inorganic orthophosphate.
7 from in-line geometry with respect to bound orthophosphate.
8 e and water (or OH-) are in equilibrium with orthophosphate.
9 pyruvate, adenosine triphosphate (ATP), and orthophosphate.
10 was studied by metabolic labeling with (32)P-orthophosphate.
11 eady state exceeded by 3-fold that of [(32)P]orthophosphate.
12 Mv1Lu cells) metabolically labeled with [32P]orthophosphate.
13 e cells were metabolically labeled with [32P]orthophosphate.
14 GA and increased resistance to the inhibitor orthophosphate.
15 ractable phosphorus, but only if measured as orthophosphate.
16 and a decreased sensitivity to the inhibitor orthophosphate.
17 n45.6 and connexin56, were labeled with [32P]orthophosphate.
18 bolically labeled with 32P-labeled inorganic orthophosphate.
19 vivo labeling of PS120/NHE3V cells with [32P]orthophosphate.
20 hydrolyzed concomitantly to produce ADP and orthophosphate.
21 residue(s) when excised shoots were fed [32P]orthophosphate.
22 y in vivo labelling of these cells with 32 P-orthophosphate.
23 by a nucleophilic water molecule to release orthophosphate.
24 ons than the corresponding condition without orthophosphate.
25 o generate flavonoid monophosphates, AMP and orthophosphate.
26 o other conditions at pH 6.5 and pH 9.0 with orthophosphate.
27 the greatest amount of NaOH-EDTA extractable orthophosphate.
28 owed soil contained lowest concentrations of orthophosphate.
29 diacylglycerol (DAG), dihydroxyacetone, and orthophosphate.
30 iphosphates to nucleoside monophosphates and orthophosphates.
31 )), which are more soluble and reactive than orthophosphates.
32 lts in protein precipitation, the effects of orthophosphate (0-64 mM) addition to sodium caseinate so
35 A resolution), in a complex with Mg(2+) and orthophosphate (1.8 A resolution), and in a complex with
38 ne-seeking radiopharmaceuticals, such as 32P-orthophosphate, 89Sr-chloride, 186Re-1,1 hydroxyethylide
41 sed the year-long impacts of full-scale DWDS orthophosphate addition on both the microbial ecology an
44 total bacterial density were observed after orthophosphate addition, likely driven by a 2 log 10 inc
46 sence of different phosphate anions, such as orthophosphate, adenosine triphosphate, and tripolyphosp
48 he similarity in pain relief afforded by 32P-orthophosphate and 89Sr-chloride, this hypothesis is exa
52 anic polyphosphate (polyP) is the polymer of orthophosphate and can be found in all living organisms.
53 vivo by pulse-labeling HeLa cells with [32P]orthophosphate and chasing using three different techniq
55 At neutral pH, products of the reaction (orthophosphate and fructose 6-phosphate) bind to the act
56 used to determine the binding force between orthophosphate and iron (oxy)hydroxide that was coated o
57 in the raw activated sludge was dominated by orthophosphate and long-chain polyphosphates, whereas in
58 lar techniques and supplied them with (33) P-orthophosphate and O. vulgatum sporophytes with (14) CO2
59 C phosphorylation sites incorporated [(33)P]-orthophosphate and showed a progressive decrease with no
60 proteins, RASM cells were labeled with [32P]orthophosphate and stimulated with 100 nmol/L Ang II for
62 t 2B7 incorporated immunoprecipitable [(33)P]orthophosphate and that 2B7His, previously expressed in
63 egates were governed by the concentration of orthophosphate and the aggregates consisted of all casei
65 ic degradation of long-chain phosphates into orthophosphate and trimetaphosphate whereas heating the
68 x formed as a result of the reaction between orthophosphates and molybdates ions where ascorbic acid
69 ntly validated through the quantification of orthophosphates and total dissolved phosphorus in pollut
70 ) by Lp(a)-associated enzymes helps generate orthophosphate, and apolipoprotein(a) blocks plasmin-ind
71 -1beta (10 U/ml, 4 h) were labeled with [32P]orthophosphate, and E-selectin was immunoprecipitated us
72 te, hydrocerussite, chloropyromorphite, lead orthophosphate, and lead oxide solids; however, in the p
73 the other hand, AMP, in the presence of GDP, orthophosphate, and Mg(2+), adopts the binding mode of a
74 itivity to its negative allosteric effector, orthophosphate, and more stable interactions between lar
76 rophosphate, and phosphoenolpyruvate to ATP, orthophosphate, and pyruvate and provides diverse functi
78 tosomes were metabolically labeled with [32P]orthophosphate, and solubilized homogenates were subject
79 the significant clinical experience with 32P-orthophosphate, and the similarity in pain relief afford
80 reveals new insight into the consequences of orthophosphate application on the DWDS microbiome and hi
81 udy, we investigated the mechanistic role of orthophosphate as a corrosion inhibitor in controlling l
84 very low soil solution concentration of free orthophosphate, as they contain high concentrations of s
85 H from 6.5 to 9.0 and the addition of 6 mg/L orthophosphate at pH 6.5 and 9.0 slowed down the copper
86 emoval of two terminal phosphate moieties as orthophosphate (Bacillus subtilis) or pyrophosphate (Esc
88 high concentrations of Ca(2+) and inorganic orthophosphate, but pH and/or other ligands for Ca(2+) ,
89 itates resulted in gradual adsorption of the orthophosphate, causing re-dispersion of the casein-iron
90 d of a hitherto unknown sodalite-type silver orthophosphate cluster (SOC) {(Ag(3) PO(4) )(8) }, remin
91 phosphates, supports an in vivo role for the orthophosphate complex of Mn(2+) in resistance to oxidat
93 ased from sediments, leading to bottom water orthophosphate concentrations increasing by a factor of
94 es isolated from induced plants had a higher orthophosphate content than granules from noninduced con
96 amine the effects of copper dose (0-2 mg/L), orthophosphate corrosion control agent, and water heater
100 t run backward synthesizing ATP from ADP and orthophosphate; (d) that its mechanism is a ping-pong on
101 ng of wild-type and mutant B cells with [32P]orthophosphate demonstrated that each of the RFX subunit
102 aortic endothelial cells labeled with [(32)P]orthophosphate demonstrated that only phosphoserine was
104 avage of triphosphate into pyrophosphate and orthophosphate did not occur, indicating that triphospha
107 e surface soils showed higher proportions of orthophosphate diesters under paddy than under non-paddy
109 emperatures with altered amounts of pyruvate orthophosphate dikinase (PPDK) and Rubisco or altered pr
110 mbined with the cytosolic/plastidic pyruvate orthophosphate dikinase (PPDK) catalyze two key steps du
111 horibosyl transferase (APRTase) and pyruvate orthophosphate dikinase (PPDK) convert adenine to ATP fo
117 included the two known isoforms of pyruvate orthophosphate dikinase (PPDK), large and small subunits
120 se in addition to the reduction of pyruvate, orthophosphate dikinase activity in o2 endosperm is comp
121 specific) and the genes that encode pyruvate orthophosphate dikinase and phosphoenolpyruvate carboxyl
124 g phosphoenolpyruvate carboxylase, pyruvate, orthophosphate dikinase, and the 2'-oxoglutarate/malate
127 Both in vivo radiolabeling (using [(32)P]orthophosphate) followed by thin-layer or high-performan
130 '-labeling of the adducts by transfer of 32P-orthophosphate from [gamma-32P]ATP mediated by polynucle
131 5'-end-dependent RNA degradation by removing orthophosphate from the 5'-diphosphorylated transcripts.
133 hosphatase and atypically releases inorganic orthophosphate from triphosphates instead of pyrophospha
135 e-dispersed soluble complexes of casein-iron-orthophosphate generated using this process could be use
136 delivered by meteorites, can be oxidized to orthophosphate, generating thiophosphate in the process.
137 lyphosphate (polyP) is an anionic polymer of orthophosphate groups linked by high energy bonds that t
138 hibitor blends (Zn+orthophosphate and Zn+NOM+orthophosphate) had stronger inhibitory effects compared
141 iosynthesis of monoselenophosphate, AMP, and orthophosphate in a 1:1:1 ratio from selenide and ATP.
145 rporation of 18O from H218O exclusively into orthophosphate in the overall selenide-dependent reactio
146 equivalent labeling of the protein with [32P]orthophosphate in the presence and absence of cyclohexim
147 endent inhibition of immunodetectable [(33)P]orthophosphate in UGTs and protein kinase Cepsilon (PKCe
148 replication by metabolic labeling with [32P]orthophosphate in vivo and obtained direct evidence that
150 ar UGT1A7 and UGT1A10 activities and of [33P]orthophosphate incorporation into immunoprecipitable pro
151 n of protein kinase A (PKA) increased [(32)P]orthophosphate incorporation into perilipin 5 by 2-fold,
153 mer-requested sampling after the addition of orthophosphate indicated below detection levels for 59%
154 1 followed by metabolic labeling with [(32)P]orthophosphate indicated that p42(mapk/erk2) phosphoryla
156 ctrometry analyses confirmed that the pH and orthophosphate inhibited copper corrosion with different
160 inase C, also enhanced incorporation of [32P]orthophosphate into TTF-1 protein; however, the DNA bind
163 ymatic hydrolysis of these esters to produce orthophosphate is often a required reaction preceding ph
165 cies are long-term labeled in vivo with [32P]orthophosphate, isolated in a highly selective way, enzy
166 tes and eukaryotes synthesize long chains of orthophosphate, known as polyphosphate (polyP), which fo
167 By immunoprecipitation carried out on [(32)P]orthophosphate-labeled AtT-20 pituitary cells stably exp
169 Separating tryptic peptides from [(32)P]orthophosphate-labeled cells and analyzing the phosphope
174 increased in response to TNFalpha in [(32)P]orthophosphate-labeled macrophages, although the level o
175 tion and ODC immunoprecipitation from [(32)P]orthophosphate-labeled NHEK lysates showed that a phosph
176 as confirmed by showing a 2-fold increase in orthophosphate-labeled Sp1 with EGF and okadaic acid.
177 or protein was markedly stimulated when [32P]orthophosphate-labeled Swiss 3T3 cells or CHO-mBR1 cells
189 mass spectrometry, mutagenesis, and [(32)P] orthophosphate labeling to identify that all five hydrox
192 radioimmunoprecipitation of lysates of [32P]-orthophosphate-labelled infected HeLa cells with anti-In
193 osphorylation of hERG was measured by [(32)P]orthophosphate labelling of immunoprecipitated protein w
194 n intact neutrophils was confirmed by [(32)P]orthophosphate loading, followed by fMLP stimulation in
196 consists of an aqueous suspension of silver orthophosphate microparticles under UV illumination, in
198 s research confirmed that nonadherent Pb(II)-orthophosphate nanoparticles were an important form of P
201 After intravenous administration of 32P-orthophosphate or 90Y-citrate in Swiss Webster mice, DNA
202 ubstrate binding was not inhibited by either orthophosphate or glycerol 3-phosphate, indicating that
203 lowing metabolic labeling of cells with [32P]orthophosphate or in vitro in phosphorylation assays wit
204 tory effects compared to each inhibitor (Zn, orthophosphate or NOM) alone, whereas Zn+NOM showed a le
206 sensing protocol allows the determination of orthophosphates over the range from 0.5 to 20 mug L(-1)
207 leaf phosphoenolpyruvate carboxylase [PEPC; orthophosphate:oxaloacetate carboxy-lyase (phosphorylati
208 , and phosphoenolpyruvate carboxylase [PEPC; orthophosphate:oxaloacetate carboxy-lyase (phosphorylati
209 relationships between five leaf P fractions (orthophosphate P, P(i); lipid P, P(L); nucleic acid P, P
210 ur assay, malachite green is used to measure orthophosphate (P(i)) concentrations after degradation b
211 ly TbIP(3)R disclosed that luminal inorganic orthophosphate (P(i)) or pyrophosphate (PP(i)), and neut
212 erichia coli detects environmental inorganic orthophosphate (P(i)) to regulate genes of the phosphate
213 nversion of adenosine 5'-triphosphate (ATP), orthophosphate (P(i)), and pyruvate with adenosine 5'-mo
215 d-enabled Donnan dialysis process to recover orthophosphate (P(V)) from alum-laden waste activated sl
217 dant complexes with small metabolites (e.g., orthophosphate, peptides), which exhibit narrow EPR sign
218 bacteria, P is acquired mainly as inorganic orthophosphate (Pi) and assimilated into adenosine triph
220 olism and physiology, and to the pathways of orthophosphate (Pi) entry into the root, which increase
224 the ability to convert phosphite (Phi) into orthophosphate (Pi) offers an alternative selectable mar
225 However, plants can only acquire inorganic orthophosphate (Pi), meaning global crop production is f
228 oiting the enzymatic reaction of maltose and orthophosphate (PO(4)(3-)) in the presence of maltose ph
229 dominated blooms to nutrient amendments with orthophosphate (PO4) and inorganic and organic forms of
230 kinesis, we labeled eggs in vivo with [(32)P]orthophosphate, prepared cortices, and mapped LC20 phosp
234 haracterization of inorganic polyphosphates [orthophosphate, pyrophosphate, tripolyphosphate, trimeta
235 nts that suggest phosphate species including orthophosphates, pyrophosphates, and adenosine phosphate
236 nversion for various P(V) sources, including orthophosphates, pyrophosphoric acid, Na(3)P(3)O(9) and
237 rgy transfer, co-immunoprecipitation, [(32)P]orthophosphate radiolabeling, and measurement of lipolys
238 ynamics simulations further corroborate that orthophosphates readily cluster in aqueous solutions.
239 iron to sodium caseinate solution containing orthophosphate reduced the diffusible phosphorus content
242 t to rare earth sesquioxides (RE(2)O(3)) and orthophosphates (REPO(4)) using DFT computations with th
243 ions such as maintaining water chemistry and orthophosphate residuals and to ensure comparability to
244 osphates (polyP) consist of linear chains of orthophosphate residues, linked by high-energy phosphoan
247 dition of iron to caseins in the presence of orthophosphate results in the formation of colloidal str
248 ciated GAPDH by in vivo labeling with [(32)P]orthophosphate revealed the presence of multiple phospho
249 stressed HeLa cells, labeled in vivo by [32P]orthophosphate, revealed four major phosphopeptides A to
250 of epimastigotes labeled for 3 h with (32)P-orthophosphate showed a significant incorporation of the
251 est importance, in vivo labeling with [(32)P]orthophosphate showed that the tryptic phosphopeptide ma
252 egions, with a corresponding increase in the orthophosphate signal, as compared to unhydrolyzed extra
253 The addition of different concentrations of orthophosphate solution to the casein-iron precipitates
259 of a novel assay procedure for quantitating orthophosphate that is extremely sensitive, reproducible
260 rganic polyphosphates are linear polymers of orthophosphate that modulate blood clotting and inflamma
261 pared with those obtained previously for 32P-orthophosphate, the radiochemical 117mSn(4+)DTPA yields
262 -01 and NIH 3T3 cells were labeled with [32P]orthophosphate to investigate COX phosphorylation in viv
263 lved in these responses, we used radioactive orthophosphate to pulse-label suspension-cultured cells
264 than 600 Da and the reduced binding force of orthophosphate to WEOM-adsorbed iron (oxy)hydroxide AFM
265 in polyphosphate metabolism and V-ATPase in orthophosphate transport were absent from CAP IB HKU-1.
266 e (Omegacalcite = 13), demonstrated that Zn, orthophosphate, tripolyphosphate, and hexametaphosphate
269 complete digestion of the oligomer sample to orthophosphate using acid at high temperature and subseq
270 , all forms of phosphorus are converted into orthophosphates via sample digestion (heating and acidif
271 ssed in transfected cells labeled with [32P] orthophosphate was phosphorylated following the addition
275 pH buffer with strong complexing properties (orthophosphate) was employed in the background electroly
276 A model distribution system, dosed with orthophosphate, was used to evaluate the effect of corro
279 totic activation is repressed by addition of orthophosphate, which significantly increases cellular p
280 f divalent cations, fructose 6-phosphate and orthophosphate, which together stabilize an R-state conf
282 )] which is achieved by activation of [(32)P]orthophosphate with ethyl isocyanate followed by aminoly
284 W complexes with nitrogenous metabolites and orthophosphate, with negligible EPR signal from Mn(2+) o
285 vailable in Enceladus's ocean in the form of orthophosphates, with phosphorus concentrations at least
287 titation of radiolabeled ATP and radioactive orthophosphate without the generation of large quantitie