ライフサイエンスコーパス: oxygen affinity

1 the modification and restored the predicted oxygen affinity.

2 anion binding site could result in increased oxygen affinity.

3 ses the hypothermia-dependent increase in Hb oxygen affinity.

4 reas alpha+M exhibits a significantly higher oxygen affinity.

5 emoprotein, named cyanoglobin, that has high oxygen affinity.

6 asparagine in an attempt to engineer higher oxygen affinity.

7 s, despite greater than 40-fold increases in oxygen affinity.

8 maintain cooperativity, but possess lowered oxygen affinity.

9 thus reduce sulfhydryl reactivity as well as oxygen affinity.

10 blood cell (RBC) sickling is to increase HbS oxygen affinity.

11 rm and that dissociation would lead to lower oxygen affinity.

12 mal hemoglobin and DecHb have a very similar oxygen affinity.

13 eutically effective than drugs that increase oxygen affinity.

14 n (HbF) with adult Hb (HbA), which has a low oxygen affinity.

15 ickling is largely offset by the increase in oxygen affinity.

16 d haemolysis, an effect not due to increased oxygen affinity.

17 ed dynamics has been associated with lowered oxygen affinity.

18 tion in the full-length enzyme did not alter oxygen affinity.

19 oes not show an inverse correlation with the oxygen affinity.

20 olecular modification, molecular volume, and oxygen affinity.

21 HP causing a more pronounced decrease in its oxygen affinity.

22 viously studied rHb(alphaL29F) exhibits high oxygen affinity.

23 oglobin Jamaica Plain," had severely reduced oxygen affinity.

24 state, along with a significant increase in oxygen affinity.

25 cs of reduction are directly proportional to oxygen affinity.

26 inant hemoglobin had significantly different oxygen affinities.

27 ssessment of measures of fetal and placental oxygen affinities.

28 and two hemoglobin solutions with different oxygen affinities.

29 ponded to DO levels in accordance with their oxygen affinities.

30 igand, which accounts for its unusually high oxygen affinity (222 microm(-1)).

31 synthetic allosteric modifier of hemoglobin-oxygen affinity, a 2-[4-[[(3,5-disubstituted anilino)car

32 ies of cell-free Hb, including cost, overall oxygen affinity, alterations in cooperativity, and ready

33 lpha V96W, beta N108K), which has the lowest oxygen affinity among the hemoglobins studied, has the g

34 oxygenases (2-OGDDs), but they vary in their oxygen affinities and hence their ability to sense oxyge

35 n leads to approximately 3-fold increases in oxygen affinity and 4-6-fold decreases in CO affinity.

36 s 50% saturated (P50 ) is a measure of blood oxygen affinity and a gauge of the tolerance of animals

37 This results in an 8-fold increase in oxygen affinity and a marked decrease in cooperativity.

38 beta 1 subunit interface can affect both the oxygen affinity and cooperativity of the oxygenation pro

39 unpolymerized sickle hemoglobin (HbS), whose oxygen affinity and cooperativity profile are equal to t

40 of the heme group, and substantially reduces oxygen affinity and cooperativity.

41 en binding experiments reveal an increase in oxygen affinity and decrease in cooperativity as the con

42 in response to S-nitrosation, with increased oxygen affinity and decreased cooperativity.

43 detectable amounts of polymer have decreased oxygen affinity and decreased deformability.

44 ickle cell disease (SCD), thereby increasing oxygen affinity and decreasing HbS polymerization and RB

45 The dual activities of increasing HbS oxygen affinity and directly inhibiting deoxy HbS polyme

46 These surface substitutions markedly reduce oxygen affinity and even confer cooperativity, because a

47 lphaV96W, betaN108K), but still exhibits low oxygen affinity and good cooperativity.

48 rHb(alphaV96W, betaN108K) exhibits low oxygen affinity and high cooperativity and also ease of

49 t hemoglobins (rHbs) with moderately lowered oxygen affinity and high cooperativity compared to human

50 uced such a mutation into our rHb having low oxygen affinity and high cooperativity.

51 tructure, we will have a hemoglobin with low oxygen affinity and high cooperativity.

52 important functional properties, such as low oxygen affinity and high cooperativity.

53 arate; the modified hemoglobin has increased oxygen affinity and lacks cooperativity.

54 b(beta L105W) does indeed possess a very low oxygen affinity and maintains normal cooperativity (P(50

55 he monomeric hemoglobins with relatively low oxygen affinity and one monomeric hemoglobin of intermed

56 both binding sites, there are differences in oxygen affinity and oxyferrous state lifetime that may b

57 They differ from each other only in oxygen affinity and oxygen dissociation rate constants,

58 tramolecular disulfide bond that affects its oxygen affinity and protein stability.

59 The increased oxygen affinity and R-state character that result from S

60 etaL28F), and rHb(betaL28W) exhibit very low oxygen affinity and reduced cooperativity compared to th

61 of C-terminal residues in hemoglobin raises oxygen affinity and reduces both cooperativity and the B

62 meric structure of hemoglobin and its normal oxygen affinity and vasoactivity.

63 ccurring abnormal human hemoglobin with high oxygen affinity and very low cooperativity, are quite di

64 pentacoordinate ferrous heme iron, moderate oxygen affinity, and a relatively rapid oxygen dissociat

65 ned in a reduced state, necessary for normal oxygen affinity, and incapable of oxygen-linked NO stora

66 ibits increased anion sensitivity, increased oxygen affinity, and increased ease of oxidation, but wi

67 globin with a hexacoordinate heme iron, high oxygen affinity, and slow oxygen dissociation rate const

68 noble metal atoms on their surface, refining oxygen affinity, and tuning adsorption of the critical o

69 depends on which OTU predominates given that oxygen affinities are species-specific, and this should

70 Oxygen affinity, as measured by P(50), did not respond t

71 esence of a transition between two different oxygen affinities at the beginning and end of the isothe

72 ltaneously maintaining thermal stability and oxygen affinity at levels well suited to respiratory gas

73 ared to rHb (beta N108Q), but exhibits lower oxygen affinity at pH below 7.4 and good cooperativity a

74 n and nitrite-oxidizing Nitrospina with high oxygen affinity (based on isotopic and omic data).

75 tribute to adaptive differentiation in blood-oxygen affinity between high- and low-altitude populatio

76 a large and significant part of the shift in oxygen affinity brought about by anion binding occurs as

77 the first PAS domain strongly increased the oxygen affinity but essentially prohibited autophosphory

78 bin are too low to affect overall hemoglobin oxygen affinity, but augmented NO transport to the micro

79 al human hemoglobin variant that has lowered oxygen affinity, but unaltered cooperativity and anion s

80 trength and if changes in fetal or placental oxygen affinities can be detected in time to allow for e

81 modifications are introduced that lower the oxygen affinity, can limit the safety of these proteins.

82 While R state haemoglobin has an oxygen affinity close to that of isolated subunits, the

83 ately 10(-)3 Torr) while Hb Lucina II has an oxygen affinity comparable to that of Mb (P50 = 0.13 Tor

84 ypoxic placentae exhibited higher hemoglobin-oxygen affinity compared to the control.

85 ess, conversion of this Tyr to Phe increases oxygen affinity considerably, suggesting that hydrogen b

86 This taxon had an apparent oxygen affinity constant lower than that of the full-sca

87 The increase in SS RBC oxygen affinity correlated with the NO concentration.

88 In contrast to the very high oxygen affinities displayed by most hexacoordinate hemog

89 that greater mass in the heme pocket lowers oxygen affinity due to impaired movement of the heme iro

90 Meanwhile, high oxygen-affinity elements of Mg and Ti well stabilize the

91 The decrease in oxygen affinities for Hb F gammaG1V, gammaS143H with inc

92 features collectively suggest a low apparent oxygen affinity for the comammox Nitrospira-dominated bi

93 d that there are several regions of enhanced oxygen affinity for the protein both on the surface and

94 oxygenated T-state tetramer to assume higher oxygen affinity forms (T', T", T"'...) with less steric

95 previously reported), Val, and Tyr increases oxygen affinity from 8- to 100-fold over that of the wil

96 the engineered Trp residue, and the lowered oxygen affinity had been attributed to a stabilization o

97 The combination of low oxygen affinity haemoglobin and reduced haemoglobin conc

98 served P(50) values for high, normal and low oxygen-affinity haemoglobin variants (13, 26 and 39 mmHg

99 disease with drugs that increase hemoglobin oxygen affinity has come to the fore with the US Food an

100 Membrane modulation of hemoglobin oxygen affinity has particularly interesting implication

101 -phospho propylation first to generate a low oxygen affinity Hb, HPPr-HbA.

102 globin concentration and P(50) , an index of oxygen affinity [Hb = -0.3135(P(50) ) + 23.636].

103 minoethanethiol) dioxygenase (ADO), as a low oxygen affinity (high-K (m)O(2)) amino-terminal cysteine

104 On the basis of its low oxygen affinity, high cooperativity, and stability again

105 rHb (beta N108Q) exhibits low oxygen affinity, high cooperativity, enhanced Bohr effec

106 Tuna species display a wide range of blood oxygen affinities (i.e., P50 values) and therefore may b

107 gest that RSR13-BC, by decreasing hemoglobin oxygen affinity, improves oxidative metabolism and prese

108 Increased osmotic pressure, which lowers the oxygen affinity in human hemoglobin, raises the oxygen a

109 Thus, high oxygen affinity in leghemoglobin is established by a fav

110 ans possess Hb that shows a profound drop in oxygen affinity in the presence of bicarbonate ions.

111 e that the cross-link introducing the lowest oxygen affinity in the two singly cross-linked species a

112 en side chain packing in the heme pocket and oxygen affinity, indicating that greater mass in the hem

113 of distinct hemoglobin isoforms with graded oxygen affinities is expected to broaden the permissible

114 loride normal cooperativity is restored, and oxygen affinity is further lowered because the shape of

115 as compared to Hb A, suggesting that higher oxygen affinity is likely to be determined by the heme p

116 lain the fact that no comparable reversal of oxygen affinity is observed under identical conditions.

117 ture therapies, particularly those where the oxygen affinity is perturbed.

118 In addition, single-cell oxygen affinity is positively correlated with Hb concent

119 xplain why Hb Ascaris has one of the highest oxygen affinities known (P50 approximately 10(-)3 Torr)

120 GWS1B under oxygenic conditions by subduing oxygen affinity, leading to 25% (E40K), 11% (T29A), and

121 d Hb Felix) retains cooperativity and has an oxygen affinity like that of HbA both in the presence an

122 variants at the beta93 position show higher oxygen affinity, lower cooperativity, and reduced Bohr e

123 The presence of 0.9 g/dL of high oxygen affinity MalPEG-Hb improves microvascular blood f

124 synthetic allosteric reduction in hemoglobin-oxygen affinity may be a new and important therapeutic s

125 Functional characterization included oxygen affinity measurements, CO combination rates, and

126 The rationale was that the high oxygen affinity MHOAH Hb, the lower oxygen affinity of H

127 gesting that hydrogen bonding is involved in oxygen affinity modulation.

128 n of Thr-72-->Val in the interface increases oxygen affinity more than 40-fold with a surprising enha

129 ult recombinant hemoglobin (rHb A) and a low-oxygen-affinity mutant recombinant hemoglobin, rHb(alpha

130 The overlap in the oxygen affinities of ammonia oxidation and denitrificati

131 In contrast, oxygen affinities of Hb F gammaG1V, gammaE5P, gammaS143H

132 The recombinant double mutant has an oxygen affinity of 10 mm Hg, which is identical to that

133 Although the oxygen affinity of blood is well-understood and routinel

134 The extremely high oxygen affinity of Ctb makes it unlikely to function as

135 The basis for the high oxygen affinity of cyanoglobin was investigated through

136 constraints that are responsible for the low oxygen affinity of deoxyhemoglobin.

137 We measured the oxygen affinity of each oxidase, using a highly sensitiv

138 Using the strong oxygen affinity of gadolinium, we design a model system

139 Oxygen affinity of Hb F gammaG1V, gammaS143H in the abse

140 the high oxygen affinity MHOAH Hb, the lower oxygen affinity of Hb S Antilles than Hb S (P50 approxim

141 To further modulate the oxygen affinity of Hb, the alpha alpha-fumaryl cross-bri

142 gle Tyr-41 and Asp-82 constructs lowered the oxygen affinity of HbS but had little or no effects on a

143 ing 5-hydroxymethyl-2-furfural, increase the oxygen affinity of hemoglobin (Hb) and strongly inhibit

144 minal 11-residue synthetic peptide lower the oxygen affinity of hemoglobin but effects on cooperativi

145 The method takes advantage of the reduced oxygen affinity of hemoglobin polymer to infer polymer c

146 low serum erythropoietin (EPO) level, normal oxygen affinity of hemoglobin, and typically autosomal d

147 are primarily responsible for modulating the oxygen affinity of hemoglobin.

148 is physiologically relevant in lowering the oxygen affinity of heterodimeric sGC and, therefore, sta

149 ructural insights led to the hypothesis that oxygen affinity of lamprey hemoglobin is distally regula

150 specific features of various NOB (e.g., high oxygen affinity of Nitrospira, tolerance to chemical inh

151 anges in chloride concentration, whereas the oxygen affinity of r Hb Presbyterian exhibits a pronounc

152 The oxygen affinity of r Hb Yoshizuka is insensitive to chan

153 The oxygen affinity of R206A BjFixL (Kd approximately 350 mi

154 s of nitric oxide (NO) gas would augment the oxygen affinity of RBCs containing homozygous HbS (SS).

155 nds are the structural basis for the lowered oxygen affinity of rHb(alpha 96Val-->Trp), and discuss t

156 gen affinity in human hemoglobin, raises the oxygen affinity of Scapharca hemoglobin regardless of wh

157 inhalation has been reported to increase the oxygen affinity of sickle cell erythrocytes.

158 Thus, low concentrations of NO augment the oxygen affinity of sickle erythrocytes in vitro and in v

159 ge influence on the chemical composition and oxygen affinity of supported nanoparticles under X-ray i

160 explanation for the difference in hemoglobin oxygen affinity of the two quaternary structures.

161 alassemia have a significant decrease in the oxygen affinity of their hemoglobin, that is an increase

162 The oxygen affinity of these mutants is lower than that of h

163 e, indicating a possible reason for the high oxygen affinity of this derivative.

164 The oxygen affinity of this Hb is insensitive to DPG and IHP

165 The principal effect controlling the oxygen affinity of vertebrate haemoglobins (Hbs) is the

166 derstand the variability and determinants of oxygen affinity on a cellular level, we have developed a

167 ls that inhibit polymerization by increasing oxygen affinity, one of them (GBT440) also inhibits sick

168 iometry (ADP/O ratios), coupling efficiency, oxygen affinity, or ADP respiratory responses, acidosis

169 The oxygen affinity (P(50) = 0.45 Torr) is similar to that o

170 ve cation exchange chromatography, exhibited oxygen affinity (P(50)) values of 14.5, 12.1, and 15.5 T

171 ecies binds oxygen cooperatively with a high oxygen affinity (P50 = 4.8 torr at pH 7.4).

172 were bred into MHOAH mice that express high oxygen affinity (P50 approximately 24.5 mm Hg) rather th

173 Des-alpha-Arg141 hemoglobin had a higher oxygen affinity (P50, 5.51 mm Hg; control, 19.94 mm Hg [

174 ized to have a central role in regulation of oxygen affinity, plays at most only a small role in dict

175 e concentration of the nonpolymerizing, high oxygen affinity R (oxy) conformation of hemoglobin S (Hb

176 The oxygen affinity reflects the average of the rapidly equi

177 ansport is enabled by their remarkably lower oxygen affinity relative to human alpha chains.

178 n R and T forms with relatively high and low oxygen affinity respectively.

179 67I) and rHb (betaV67F) exhibit low and high oxygen affinity, respectively.

180 Significant reduction in oxygen affinity resulting from interactions between hete

181 senide FTJ is due to gallium arsenide's weak oxygen affinity, resulting in fewer oxygen vacancies.

182 Increased oxygen affinity results from the absence of the Phe97 si

183 he Fe(2+) to the Fe(3+) state than other low-oxygen-affinity rHbs developed in our laboratory, e.g.,

184 rcuribenzoate, and our results show that low-oxygen-affinity rHbs have a more reactive beta93Cys than

185 ore, the cooperativity index and the overall oxygen affinity seem to be correlated to the positive en

186 gammaE5P, gammaS143H, which exhibit reduced oxygen affinity, should facilitate design of efficient a

187 th more rapid kinetics occurring in the high oxygen affinity state (i.e. modification of the Cysbeta-

188 ification of the Cysbeta-93) than in the low oxygen affinity state (i.e. treatment with inositol hexa

189 lay an important role in maintaining the low-oxygen-affinity state (T state) in deoxy HbA, but a late

190 These proteins often have very high oxygen affinities stemming from very slow ligand off rat

191 ral pH range according to the proton NMR and oxygen affinity studies presented here.

192 B- and C-families also have higher apparent oxygen affinities than the A-family.

193 e of the central cavity and exhibits a lower oxygen affinity than wild-type HbA in the presence of ch

194 evolved-a noncooperative homodimer with high oxygen affinity that existed before the gene duplication

195 HbAS3 binds oxygen cooperatively and has an oxygen affinity that is comparable with fetal hemoglobin

196 of synthetic peptide to Hb, resulting in an oxygen affinity that is moderately decreased and a parti

197 tion of the second cross-linking reduces the oxygen affinity to 15.9 torr, which compares with 13.0 t

198 Monomeric invertabrate hemoglobins with high oxygen affinity usually contain a tyrosine in the distal

199 icity in the distal heme pocket can decrease oxygen affinity via steric hindrance effects while incre

200 Convergent increases in hemoglobin-oxygen affinity were pervasive among high-altitude taxa,

201 al for viability and exhibited a much higher oxygen affinity, with a K(m) value of 40 nM and a V(max)