ライフサイエンスコーパス: p115RhoGEF

1 ression of constitutively active Galpha(13), p115RhoGEF, or RhoA mimicked lysophosphatidic acid (LPA)

2 naling by directly inhibiting the Galpha(13)-p115RhoGEF interaction.

3 The mechanism for the G alpha(13)/p115RhoGEF interaction is not well understood.

4 S interaction are preserved in the alpha(13)/p115RhoGEF interaction, there are also significant diffe

5 l cross-talk exerted from the LPA/Galpha(13)/p115RhoGEF/RhoA pathway to the beta(2)-adrenergic recept

6 IV collagen, whereas a constitutively active p115RhoGEF restored the arachidonic acid stimulation in

7 nstream mediators Galpha(12), Galpha(13) and p115RhoGEF regulated cell viability and was required for

8 eam RhoA activators including Galpha(13) and p115RhoGEF, with Galpha(13)-induced Rho.GTP loading inhi

9 beta(2)AR activation, both betaArrestin2 and p115RhoGEF translocate to the plasma membrane, with conc

10 unoblot analyses indicate that both CD44 and p115RhoGEF are expressed in MDA-MB-231 cells and that th

11 p38 MAPK-dependent association of HSP27 and p115RhoGEF.

12 s, PDZ RhoGEF, which, together with LARG and p115RhoGEF, links the G(12/13) family of heterotrimeric

13 oGEF, leukemia-associated RhoGEF (LARG), and p115RhoGEF.

14 PDZ-RhoGEF, LARG, and p115RhoGEF are members of a newly identified family of R

15 for Rho that includes PDZ-RhoGEF, LARG, and p115RhoGEF exhibits a unique structural feature consisti

16 olling the activity of PDZ-RhoGEF, LARG, and p115RhoGEF, which involves homo- and hetero-oligomerizat

17 tide exchange factor (GEF)), PDZ-RhoGEF, and p115RhoGEF augmented interaction between activated Galph

18 GEF within 1 min of thrombin stimulation and p115RhoGEF phosphorylation was dependent on PKCalpha.

19 t RhoA and JNK signaling components, such as p115RhoGEF and MKK4.

20 ates the GEF activity of RH-RhoGEFs, such as p115RhoGEF, has not yet been fully elucidated.

21 12/13 and PKCalpha pathways that converge at p115RhoGEF.

22 tly stimulates the exchange activity of both p115RhoGEF and leukemia-associated RhoGEF but not PDZ-Rh

23 Inhibition of PKCalpha-dependent p115RhoGEF phosphorylation prevented the thrombin-induce

24 Through its RH domain, p115RhoGEF also functions as a GAP for G alpha(13).

25 hrombin-mediated signaling or their effector p115RhoGEF involved in Rho activation caused MT disassem

26 on of the guanine nucleotide exchange factor p115RhoGEF, which contains a regulator of G-protein sign

27 rough the guanine nucleotide exchange factor p115RhoGEF.

28 specific guanine nucleotide exchange factor (p115RhoGEF) in human metastatic breast tumor cells (MDA-

29 ain of a guanine nucleotide exchange factor, p115RhoGEF, inhibited thrombin-dependent phosphorylation

30 specific guanine nucleotide exchange factors p115RhoGEF and LARG (leukemia-associated RhoGEF), fails

31 regulator of G protein signaling (RGS) from p115RhoGEF or a dominant negative RhoA(T19N).

32 Recently, we identified p115RhoGEF, one of the guanine nucleotide exchange facto

33 activation of p115RhoGEF cascades, including p115RhoGEF itself, RhoA, Cdc42, and Rac1.

34 ed its ability to bind to p115RhoGEF, induce p115RhoGEF recruitment to the PM, and activate Rho-depen

35 h the RH domain of p115RhoGEF or full-length p115RhoGEF.

36 more dramatic in the context of full-length p115RhoGEF.

37 ns (phospholipase C- inverted question mark, p115RhoGEF) and a growing family of regulators of G prot

38 Expression of the dominant negative p115RhoGEF was able to inhibit activation of both RhoA a

39 ues in Galpha(13) that mediate activation of p115RhoGEF are identified.

40 om G alpha(13) to JNK requires activation of p115RhoGEF cascades, including p115RhoGEF itself, RhoA,

41 e data suggest that the exchange activity of p115RhoGEF is stimulated allosterically by Galpha(13) an

42 ation of the nucleotide exchange activity of p115RhoGEF on Rho by Galpha(13).

43 ot G alpha(i/12), stimulated the activity of p115RhoGEF.

44 With the aid of p115RhoGEF-RGS, G(12) and G(13) minigenes to inhibit G(1

45 sing shortened RH-RhoGEF DH/PH constructs of p115RhoGEF/ARHGEF1, PDZ-RhoGEF (PRG)/ARHGEF11, and LARG/

46 C-epsilon nor inhibited by the RGS domain of p115RhoGEF but was blocked by expression of the RGS doma

47 ture of Galpha(13) bound to the RH domain of p115RhoGEF is also presented, which differs from a previ

48 of activated alpha(13) with the RH domain of p115RhoGEF or full-length p115RhoGEF.

49 e regulator of G protein signaling domain of p115RhoGEF to inhibit Galpha12 and Galpha13 during thymo

50 e regulator of G-protein signaling domain of p115RhoGEF, a GTPase activating protein for G12/13, also

51 lator of G protein signaling (RGS) domain of p115RhoGEF, a GTPase-activating protein for Galpha(12/13

52 for Galpha12 binding with the RGS domain of p115RhoGEF, a known G12-interacting protein that links G

53 or of G protein signaling (rgRGS) domains of p115RhoGEF and homologous exchange factors differ from t

54 of the constitutively active mutant form of p115RhoGEF (guanine nucleotide exchange factor) was foun

55 olving PKCalpha-dependent phosphorylation of p115RhoGEF.

56 ed downstream of PKCalpha phosphorylation of p115RhoGEF.

57 tivation secondary to the phosphorylation of p115RhoGEF.

58 vated alpha(13) to induce the recruitment of p115RhoGEF to the plasma membrane (PM) and to activate R

59 scaffold protein that interacts with Net1 or p115RhoGEF, two Rho-specific guanine nucleotide exchange

60 pression of dominant negative p115-RhoGEF or p115RhoGEF-specific siRNA inhibited both RhoA activation

61 RGL-RhoGEFs) that includes PDZ-RhoGEF (PRG), p115RhoGEF, and LARG, thereby regulating cellular functi

62 anine nucleotide exchange factors (RhoGEFs) (p115RhoGEF, leukemia-associated RhoGEF, and PDZ-RhoGEF)

63 binding of HA to MDA-MB-231 cells stimulates p115RhoGEF-mediated RhoA signaling and Rho kinase (ROK)

64 mutations, when placed in the context of the p115RhoGEF molecule, produce deficiencies in GAP activit

65 An extensive mutagenesis analysis of the p115RhoGEF rgRGS domain was undertaken to determine its

66 gnificantly impairs binding of Galpha(13) to p115RhoGEF as well as stimulation of GEF activity.

67 Ser (G205S) retained its ability to bind to p115RhoGEF, induce p115RhoGEF recruitment to the PM, and

68 ctor proteins and exhibited GAP responses to p115RhoGEF and leukemia-associated RhoGEF.

69 nt of G12/13 in signaling Rho activation via p115RhoGEF.

70 ho activation caused MT disassembly, whereas p115RhoGEF-specific negative regulator RGS preserved MT

71 can form homo- and hetero-oligomers, whereas p115RhoGEF can only homo-oligomerize, and that this inte

72 vide new insight into the mechanism by which p115RhoGEF is activated by Galpha(13).

73 HSP27 associated with p115RhoGEF in fatty acid-treated cells, and this associa

74 We observed that PKCalpha associated with p115RhoGEF within 1 min of thrombin stimulation and p115

75 show that betaArrestin2 forms a complex with p115RhoGEF in the cytosol in resting cells.

76 betaArrestin2 forms a complex with p115RhoGEF, a guanine nucleotide exchange factor for Rho

77 strongly suggest that CD44 interaction with p115RhoGEF and ROK plays a pivotal role in promoting Gab

78 residues important for its interaction with p115RhoGEF.