ライフサイエンスコーパス: pI

1 harge isoforms differing by as little as 0.1 pI units.

2 both angiogenic and bactericidal; RNase A-1 (pI 10.2) has neither activity.

3 o the pI of the SP, and pH values of pI - 2, pI, and pI + 2 respectively provided exceptional specifi

4 n solution, has a stoichiometry of 1:1 and a pI of 6.0-6.5.

5 160 kDa, a stoichiometry of 1:1:1:1:1, and a pI of 7.4-8.1.

6 n estimated molecular mass of 77.3 kDa and a pI value of 5.66, characteristics consistent with transf

7 s are used as internal standards to assign a pI value to each individually detected mitochondrial eve

8 nce fully developed, it can potentially be a pI-based sample fractionation tool for proteomic analysi

9 ily oxidized in cultured cells, generating a pI 5.8 isoform, but an artificial C106A mutant was not.

10 dged by native gel electrophoresis and has a pI value of 4.55, as found for most of the fungi of beta

11 ll nucleic acid-interacting proteins, have a pI of 7.94 or higher; virtually all these 12,000 protein

12 the Hbb(dminor) polypeptide, resulting in a pI of 7.85 versus 7.13.

13 ic and spectrophotometric titration showed a pI of 4.22 +/- 0.33 and apparent pK values of 6.74 +/- 0

14 and 45 degrees C, respectively and showed a pI value of 4.6.

15 he pI are often observed for proteins with a pI above 7.0 upon phosphorylation, whereas little change

16 ovo set of peptides/proteins, largely with a pI between 5.0 and 8.0 and a molecular mass distribution

17 sing of the C-terminal Abeta peptides with a pI of 5.3 in the boundary between cSer and Asp-His.

18 tion of ErA, resulting in a main peak with a pI of 7.35 and an acidic species minor peak at 7.0, both

19 ittle change is observed for proteins with a pI of approximately 5.0.

20 Zn was mainly associated to proteins of acid pIs.

21 ed adjuvant polyinosinic:polycytidylic acid (pI:C).

22 to polyriboinosinic:polyribocytidylic acid (pI:C), DCs mount a specific integrated stress response d

23 horesis demonstrated that SWP3 has an acidic pI and a molecular mass of <20 kDa.

24 Only the predominant AMACR IA has an acidic pI and contains the previously identified peroxisomal ta

25 pal tissue exposed to SH exhibited an acidic pI shift of VCP.

26 Despite an overall acidic pI, the asymmetric electrostatic charge profile suggests

27 ass from 50 to 100 kDa and a slightly acidic pI (5.4-6.8).

28 (four) were located in two strongly acidic (pI 4 to 4.9) domains in the distal N- and C-terminal reg

29 dominant B-cell epitopes within the acidic (pI 5.35) ankyrin repeat-containing 200-kDa major immunor

30 Both azurin (P. aeruginosa) (pI = 5.6) and subunit II (Cu(A) domain) of ba(3)-type cy

31 dies revealed that LAMP2 has a more alkaline pI in Pompe compared with control iPSC-CMs due largely t

32 sociated with dissolved proteins of alkaline pIs in surface seawater, while Zn was mainly associated

33 h a molecular weight of 33.38+/-0.34kDa, and pI of 6.30+/-0.15 was confirmed in samples of SOD extrac

34 eins differ widely in buffering capacity and pI and therefore the same PTMs may give rise to quite di

35 ters such as disorder, context, charges, and pI.

36 raction revealed a single spot of 34 kDa and pI 8.8.

37 itive rate associated with accurate mass and pI measurements is presented to demonstrate the utility

38 of the SP, and pH values of pI - 2, pI, and pI + 2 respectively provided exceptional specificity for

39 e., secretion signal, cysteine residues, and pI).

40 However, biases in protein size and pI resolution, as well as limitations in sample volume,

41 antigen features, such as protein weight and pI, and subcellular localizations of bacterial proteins.

42 of a few proteins with molecular weights and pI values typical of cathepsin D, E and pepsin.

43 used to electrostatically stabilize the AOx (pI 4.3) on the anode operating on direct electrochemistr

44 ynthesized AOx-AuNPs conjugate from the AOx (pI 4.5) protein (zeta, -30mV) implied good stability of

45 but precipitates at pH 7.2, its approximate pI value.

46 novel tetrapeptide scaffold [Ac-Xaa(1)-Arg-(pI)DPhe-Xaa(4)-NH2] is reported.

47 s-Arg-(pI)DPhe-Tic-NH2], and 41 [Ac-Arg-Arg-(pI)DPhe-DNal(2')-NH2] were more potent (EC50 < 73 nM) th

48 e most potent MC3R agonists, 18 [Ac-Arg-Arg-(pI)DPhe-Tic-NH2], 1 [Ac-His-Arg-(pI)DPhe-Tic-NH2], and 4

49 he-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Arg-(pI)Phe-NH2, and Ac-Arg-(pCl)DPhe-Tic-(pNO2)DPhe-NH2, rev

50 Ac-Arg-Arg-(pI)DPhe-Tic-NH2], 1 [Ac-His-Arg-(pI)DPhe-Tic-NH2], and 41 [Ac-Arg-Arg-(pI)DPhe-DNal(2')-N

51 plate contains a sequentially reversed "Arg-(pI)DPhe" motif with respect to the classical "Phe-Arg" m

52 idiotypic (Id) peptides can be displayed as pId:MHCII complexes on B cells for recognition by CD4(+)

53 termine pI of mitochondria report an average pI.

54 In general, learning-based pI prediction methods (such as Cofactor, SVM and Branca)

55 mcfp-5 resembles mcfp-3 in its basic pI and abundant 3,4-dihydroxyphenyl-L-alanine (Dopa; 30

56 Both proteins are basic (pI approximately 10) and exhibit Gly-rich peptide repeat

57 oint (pI) is particularly attractive because pI is a well-defined parameter and it is orthogonal to h

58 ntested, motivating our efforts to benchmark pI prediction methods.

59 tallized proteins and the difference between pI and reported pH at which they were crystallized.

60 Both pI and Mr. of BSA were modified upon glycation reaction.

61 g protein and peptide samples covering broad pI range.

62 roteins into the desalinator was followed by pI fractionation with MJ-CIEF in less than 1 h.

63 ation of biological molecules in solution by pI.

64 molecular mass of 51,114 Da and a calculated pI of 4.8.

65 The standard deviation of calculated pI values for unmodified peptides from the theoretically

66 nificant relationship between the calculated pI of successfully crystallized proteins and the differe

67 ed an unusual enrichment in highly cationic (pI > 9) proteins.

68 RNase A-2, the more cationic (pI 11.0), is both angiogenic and bactericidal; RNase A-1

69 In the absence of cDCs, pI:C failed to induce Th1 cell commitment and IgG2c prod

70 ructed with axes of protein mass and charge (pI) and converted to density estimates comparable across

71 C/EBPbeta and C/EBPdelta to endogenous CIITA pI and pIV.

72 a and C/EBPdelta, and their binding to CIITA pI and pIV, in correlation with inhibition of IFN-gamma-

73 Selected proteins and colored pI markers were used as model analytes.

74 omponent ampholyte buffers with well-defined pI cutoff values, controlled separation of protein mixtu

75 Fluorescein-derived pI markers are used as internal standards to assign a pI

76 Previous methods to determine pI of mitochondria report an average pI.

77 most proteins, the experimentally determined pI is very close to that predicted by the databases.

78 The determined pI's were 6.2 for Mahoney, 6.7 for MEF-1, and 5.8 for Sa

79 ides of similar molecular mass but different pI, suggesting the possibility of posttranslational modi

80 sylated protein forms separated to different pI.

81 known if naive B cells spontaneously display pId:MHCII in vivo or if BCR ligation is required for exp

82 mammals, other properties of Spot 14 (i.e., pI, subcellular localization, transcriptional control an

83 roducible fractionation of model fluorescent pI markers and proteins is achieved using single membran

84 isoelectric focusing of several fluorescent pI markers and proteins is demonstrated across pH 3.8-7.

85 strating the applicability of the method for pI determination in micropreparative procedures within a

86 rmodynamics for adding and removing HCN from pI under Titan conditions suggests that such dynamics is

87 in the discovery of compound 42 (Ac-Val-Gln-(pI)DPhe-DTic-NH(2)), a full MC3R agonist that is 100-fol

88 ermined as negatively charged glycoproteins (pI < 6) with molecular masses between 100 and 300 kDa.

89 e algae identified in the community also had pI distributions similar to 'salt-in' strategists.

90 binding of the anionic phage coat to a high pI target protein.

91 lay-nonspecific binding by the phage to high pI target proteins.

92 noantennary glycans associated with the high-pI fractions, nor were predominantly tri- and tetra-ante

93 vis-a-vis quaternary structure of this high-pI protein.

94 Higher pI peaks were found to contain N- and C-terminal heavy-c

95 ved interconversion between the dark, higher pI and bright, lower pI GFP populations is tightly contr

96 tylcarboxylic acid, Anc, JRH420-12), Ac-His-(pI)DPhe-Arg-Trp-NH2 (JRH322-18), chimeric AGRP-melanocor

97 unds at all these hMC4R SNPs include Ac-His-(pI)DPhe-Tic-(pNO2)DPhe-NH2, Ac-His-(pCl)DPhe-Tic-(pNO2)D

98 to pt-DNA versus ds-DNA, and a difference in pI for these two complexes, whereas Klentaq does not, su

99 proteins uncovered unexpected differences in pI and/or size of specific proteins thought to be highly

100 rane proteins, and proteins with extremes in pI and MW.

101 variants of monocolonal antibodies (mAbs) in pI range of 7-10.

102 yield of protein that has a broader range in pI, and reducing experimental time compared to conventio

103 DJ-1 isoforms, of which the acidic isoforms (pI 5.5 and 5.7) of DJ-1 monomer and the basic isoforms (

104 5.7) of DJ-1 monomer and the basic isoforms (pI 8.0 and 8.4) of SDS-resistant DJ-1 dimer are selectiv

105 he 4-helix bundle has a unique role, and its pI (7.8) is higher than other rod domains.

106 lubility properties, with one exception: Its pI is higher by nearly one pH unit.

107 creasing pHs, especially at pHs close to its pI (pH 4.0), indicating the occurrence of co-precipitati

108 were identified in CIITA promoters I and IV (pI and pIV).

109 exon 3 and are designated as IB (Mw 22 kDa, pI 10.31) and IIB (Mw 31 kDa, pI 9.44).

110 horter variant form of IIA (IIAs, Mw 28 kDa, pI 9.65), which lacks the 5' half of the alternative fif

111 IB (Mw 22 kDa, pI 10.31) and IIB (Mw 31 kDa, pI 9.44).

112 encodes a 288-amino acid protein (Mw 32 kDa, pI 9.6).

113 ly a single 39-kDa band and a single 39-kDa, pI 5.0 spot on one- and two-dimensional immunoblots of B

114 encodes a 382-amino acid protein (Mw 42 kDa, pI 6.07).

115 An immunoreactive protein spot (MW: 44 kDa, pI: 4.5) was microsequenced and the related cDNA was clo

116 trol the assembly of cytochrome c (12.5 kDa, pI 10.5) and azurin (13.9 kDa, pI 5.7) proteins into sep

117 c (12.5 kDa, pI 10.5) and azurin (13.9 kDa, pI 5.7) proteins into separate 3D DNA nanocages, in a pr

118 kinase, was straightforward due to the large pI shift upon multiple phosphorylation events.

119 tly all recombinant basic proteins (at least pI-1 > or = 6.94) may be purified from E. coli in a sing

120 resses GA-induced expression of a barley low pI alpha-amylase gene (Amy32b).

121 nthesized and used to selectively enrich low pI peptides from complex mixtures for MALDI-MS detection

122 s, not more than one O-glycosylated Ser, low pI and membrane location.

123 We show that enrichment of these low pI peptides allows acidic proteins to be selectively tar

124 f apoptosis protein-binding protein with low pI (Diablo) protein translocates into the cytosol during

125 ibitor of apoptosis-binding protein with low pI (Smac/DIABLO) into the cytoplasm and substantial incr

126 caspases/direct IAP binding protein with low pI from mitochondria.

127 ibitor of apoptosis-binding protein with low pI may be differentially released from the mitochondria,

128 ibitor of apoptosis-binding protein with low pI were released following the addition of TNF-alpha.

129 caspase/direct IAP-binding protein with low pI) and caspase-9 bind, is a promising strategy for inhi

130 caspases/direct IAP-binding protein with low pI), a factor that neutralizes the protective actions of

131 caspases/direct IAP-binding protein with low pI), caspase activation, and neuronal death.

132 f apoptosis protein-binding protein with low pI), caspase-3 activity (but not procaspase-3 cleavage),

133 ibitor of apoptosis-binding protein with low pI); anti-apoptotic: XIAP (X-linked inhibitor of apoptos

134 caspases/direct IAP binding protein with low pI, and apoptosis-inducing factor release), caspase acti

135 the electrostatic repulsion between the low-pI ACE2 and the heparin segments not accommodated on the

136 tetra-antennary glycans associated with low-pI fractions.

137 Acidic (lower pI) peaks were found to represent deamidated and sialyat

138 etween the dark, higher pI and bright, lower pI GFP populations is tightly controlled by differential

139 identifying peptides that tend to have lower pI values.

140 wnregulated protein in A3HtrAOE with a mass, pI and MALDI-TOF spectrum consistent with outer membrane

141 rmulation offered higher resolution (minimum pI difference ~0.07 +/- 0.02) than unmodified 6%T, 3.3%C

142 ted mitochondrial event, and a mitochondrial pI distribution is determined.

143 le distributions of individual mitochondrial pI, accurate determination of the pI of individual mitoc

144 ses of redox proteins with acidic-to-neutral pI values have been obtained on pure alkanethiol as well

145 These shift the normal pI of insulin from 5.4 to approximately 7.

146 Obtained pI's were generally in good agreement with known data, d

147 proteins show a substantial acidification of pI and require high salt concentrations for cooperative

148 dard, we have undertaken the benchmarking of pI calculation methods.

149 e of IEF markers for internal calibration of pI.

150 We speculate that the directionality of pI's intermolecular and intramolecular =N-H(...)N hydrog

151 ine protein pI followed by immobilization of pI-resolved proteins.

152 Inhibition of pI-kappaBalpha by BAY 11-7085 resulted in decreased Bcl-

153 onstrated a separation of binary mixtures of pI markers and proteins without applying any external fi

154 may give rise to quite different patterns of pI shifts in different proteins.

155 RPLC-MS data are used to construct a plot of pI vs MW for a protein mixture.

156 Therefore accurate theoretical prediction of pI would expedite such analysis.

157 n molecular weight that possessed a range of pI values were represented by molecular forms of CgA and

158 and reported, and thus literature reports of pI abound.

159 (pI) selection, but in the specification of pI progeny.

160 lative to the pI of the SP, and pH values of pI - 2, pI, and pI + 2 respectively provided exceptional

161 g dramatically increases physical display of pId:MHCII, leading to activation of Id-specific CD4(+) T

162 do not express readily detectable levels of pId:MHCII.

163 l fractionation of peptide mixtures based on pI can be accomplished by varying the pH of the aqueous

164 nd throughput for protein profiling based on pI vs MW.

165 regardless of protein characteristics (MW or pI) or abundance.

166 A protein's isoelectric point or pI corresponds to the solution pH at which its net surfa

167 ic point and (2) the introduction of peptide pI is effective in minimizing both false positive and fa

168 on for measurable adsorption and the peptide pI, and proximity of cationic groups to the surface in t

169 Panulirin is a cationic peptide (pI 9.5) composed of 48 amino acid residues (5.3 kDa), wi

170 The K(D)(2-1) increased as |pH-pI| increased, indicating that the hydrophobic effect is

171 It has a low isoelectric point (pI 1.1) and, therefore, tends to be negatively charged a

172 5) and below (pH 2.5) the isoelectric point (pI approximately 5.3).

173 cations (PTMs) on protein isoelectric point (pI) and molecular weight and displays the calculated pat

174 ted by differences in the isoelectric point (pI) and the ion exchange properties of the printed subst

175 s based on differences in isoelectric point (pI) and then identifies protein targets through immunopr

176 accurate mass and peptide isoelectric point (pI) as identification criteria, and represents a change

177 The isoelectric point (pI) cutoff in these extractions depends on the pH of the

178 luidic platforms for fast isoelectric point (pI) determinations via free-flow electrophoresis with in

179 fe style and a low median isoelectric point (pI) for all predicted proteins, suggesting a 'salt-in' s

180 ng protein with low pH of isoelectric point (pI) from the mitochondria, and the activation of caspase

181 ractionation based on the isoelectric point (pI) is particularly attractive because pI is a well-defi

182 l protein (ORF3), with an isoelectric point (pI) of 10.0 and a calculated molecular mass of 22.8 kDa.

183 tein Data Bank (PDB), the isoelectric point (pI) of a protein has been explored as a useful indirect

184 olecular weight and lower isoelectric point (pI) of Hu-recA1PI than pd-A1PI.

185 ely one pH unit below the isoelectric point (pI) of the recombinant proteins, most of the latter bind

186 uled out by comparing the isoelectric point (pI) values to the adsorption of proteins to lignin surfa

187 mine sCD44 concentration, isoelectric point (pI), and phosphorylation, respectively.

188 such as applied voltage, isoelectric point (pI), bulk pH, and bulk conductivity, on the protein trap

189 A novel continuous-flow isoelectric point (pI)-based sorting technique has been developed for prote

190 estimation of the protein isoelectric point (pI).

191 o charge inversion at its isoelectric point (pI=5.3) but remained anionic.

192 containing proteins with isoelectric points (pI values) higher than the pI of albumin, a second fract

193 ill-resolved peaks with isoelectric points (pI values) in the range 7.4 to 8.5.

194 g compounds with varying isoelectric points (pI) and pK(a) values over a range of aqueous solution pH

195 rved to exhibit distinct isoelectric points (pI) and, thus, distinct formal electrostatic charges.

196 (CIEF-WCID) method, the isoelectric points (pI) of complete intact polioviruses were determined.

197 fractions based on their isoelectric points (pI) without the use of carrier ampholytes.

198 es (i.e., those with low isoelectric points (pI)) by matrix-assisted laser desorption/ionization-mass

199 that likely have unique isoelectric points (pI), which are related to their surface compositions and

200 eawater exhibit alkaline isoelectric points (pIs of 8.10 and 8.37) and also acid Ips (4.82, 5.13, 5.4

201 the difference in their isoelectric points (pIs) after methyl esterification.

202 tigate the chain conformations of polyimine (pI), a polymer identified as one major component of poly

203 s Senseless not in the primary SO precursor (pI) selection, but in the specification of pI progeny.

204 ed peptides from the theoretically predicted pI values was on average 0.21 pH units, which is more ac

205 k at 7.0, both comparing well with predicted pI values based on the sum of protein residue pKa values

206 te to pI values consistent with predictions (pI 7.0 for one deamidation).

207 copies regarding their selection pressures, pI values and tissue expression patterns, suggesting the

208 is expressed from three distinct promoters (pI, pIII, and pIV) in a developmental and cell type-spec

209 tudy the influence of solvent pH and protein pI on the ionization responses of proteins.

210 size polyacrylamide gel to determine protein pI followed by immobilization of pI-resolved proteins.

211 was roughly 5 units higher than the protein pI.

212 olution was 4-5 units lower than the protein pI.

213 To effect this protein pI separation, chromatofocusing(CF) is employed whereby

214 liquid separation technique in which protein pI is used as the first-dimension separation parameter.

215 relate, at least qualitatively, with protein pI.

216 Many basic proteins (pI>7) and putative disease biomarkers are not identified

217 Four novel 17 kDa proteins (pI 6.5-6.8) were recognized in a proteomic screen of lys

218 ot analysis revealed that the representative pI of the 32-kDa sCD44 was 6.96 +/- 0.07 in POAG versus

219 an be optimized to generate highly resolved, pI-based fractions in solution which can be used for suc

220 The expressed protein's pI value thus becomes an important parameter together wi

221 the conjugation site, surrounding residue's pI, and electrostatic charge, may directly impact the dr

222 rboxyl to lysine substitutions, 5K RNase Sa (pI=10.2), have been determined by NMR at 20 degrees C in

223 atable groups in ribonuclease Sa (RNase Sa) (pI=3.5), and a charge-reversed variant with five carboxy

224 ified forms being fractionated into separate pI compartments.

225 that the intrinsic positive charges of SEVI (pI = 10.21) facilitate virion attachment to and fusion w

226 The enzyme showed pI of 4.1 and a single polypeptide band of 83.1kDa after

227 Several proteins with a significant pI shift between their isoforms in the patient and contr

228 other hand, required the addition of a small pI range (4-6.5) of ampholytes to improve resolution and

229 Of these, the SOD1 pI 6.0 isoform is oxidatively modified by carbonylation,

230 ration and isolated peptides within specific pI ranges from sample mixtures, where the pI range was s

231 equence analysis of a 67.8 kDa protein spot (pI 5.7) on a Coomassie-stained two-dimensional (2D) gel

232 A major protein spot (pI of 5.9 and molecular weight of 37 kDa) was seen in LN

233 e the same for both groups of these strains, pI = 3.4.

234 While such pI calculation is widely used, it remains largely untest

235 rency in Titan's atmosphere, indicating that pI could be photochemically active and drive chemistry o

236 The pI of the protein, and optimum pH/temperature of enzyme

237 The pI vs MW profile obtained from CIEF-RPLC-MS compares fav

238 The pI's of Sabin types 1, 2, and 3 viruses were 7.4, 7.2, a

239 s developed to calculate more accurately the pI values of peptides identified from experimental data.

240 idatively modified by carbonylation, and the pI 5.0 isoform is selectively accumulated in AD and PD.

241 ), and Lys (11.3 and 10.6) peptides, and the pI of BSA (4.8) and FN (5.7), were consistent with publi

242 y to the multi-lectin column (M-LAC) and the pI profiles in IEF separation.

243 eins with pI values in the same range as the pI of albumin, and a third fraction containing proteins

244 soelectric point-commonly referred to as the pI-can be defined as the point of singularity in a titra

245 At a pH value below the pI of the antibody, a nonmonotonic relationship of T(c)

246 Below the pI, the protein molecules were completely and permanentl

247 tigate the disputed relationship between the pI of a protein and the pH at which it crystallizes.

248 the early days of solution biochemistry, the pI has been recorded and reported, and thus literature r

249 inylated oligodeoxynucleotide containing the pI or pIV binding sites, and chromatin immunoprecipitati

250 These devices allow one to determine the pI of a biomolecule from a sample mixture with moderatel

251 the improved device, it was applied for the pI fractionation of yeast proteome digest into 18 fracti

252 Thus, cDCs are required for the pI:C-driven Th1 cell fate commitment but have no crucial

253 Moreover, large changes in the pI are often observed for proteins with a pI above 7.0 u

254 acterized by posttranslational change in the pI of sCD44 and hypophosphorylation, which clearly disti

255 These modifications cause changes in the pI of the protein by adding, removing or changing titrat

256 configuration allows seven fractions in the pI range 3-10 to be analyzed by RPLC-MS in 2 h.

257 V light was demonstrated by the shift in the pI value of Bax on two-dimensional gels and confirmed by

258 on of sCD44 resulted in a basic shift in the pI.

259 alitatively with trends in B except near the pI of amylase, where physical stability was minimal.

260 found that a mobile phase pH value near the pI of the zwitterionic adsorbent surface leads to full r

261 ochondrial pI, accurate determination of the pI of individual mitochondria by the use of internal sta

262 d whey was explained by the proximity of the pI of whey protein (4.9-5.2), where proteins were found

263 proteins are mostly acidic, and based on the pI determined theoretically, apparently all recombinant

264 , and a selected buffer pH, depending on the pI of the recombinant protein.

265 he electrophoretic field required to run the pI-based sorting is generated by the diffusion of buffer

266 cells or phosphorylations that can shift the pI by several pH units.

267 Using this adjusted CIEF-WCID technique, the pI of biologically hazardous components like toxins or v

268 oelectric points (pI values) higher than the pI of albumin, a second fraction containing proteins wit

269 ining proteins with pI values lower than the pI of albumin.

270 It is demonstrated that the pI for expressed intact proteins as generated by CF is a

271 ed at acidic pH, with a maximum close to the pI of actin.

272 At pH 7.1, close to the pI of the antibody, a decrease of T(c) versus ionic stre

273 ly associated with buffer pH relative to the pI of the SP, and pH values of pI - 2, pI, and pI + 2 re

274 In other cases, however, where the pI is observed to be shifted from the expected value, it

275 ic pI ranges from sample mixtures, where the pI range was simply set by the pH values of the buffer s

276 al muscle mitochondria) and to determine the pIs of other biological or nonbiological particles.

277 based program, pIMethylation, to predict the pIs for peptides with and without methyl esterification.

278 through the avidin zone regardless of their pI values.

279 This allowed the determination of their pI via multispectral imaging of protein and pH sensor fl

280 modified insulins, demonstrating that their pI values were shifted in the expected manner.

281 bunits traveled primarily according to their pI and apparently were not associated with other protein

282 Peptide fractionation according to their pI is also widely used in current proteomics sample prep

283 tionating protein samples according to their pI values without using ampholytes.

284 peptides left the focusing gel because their pIs are higher than the upper limit of the immobilized p

285 molecular mass of 72.3 kD and a theoretical pI of 8.78.

286 IEF results were consistent with theoretical pIs of methylated peptides calculated by pIMethylation.

287 type cytochrome c oxidase (T. thermophilus) (pI = 6.0) exhibit optimal voltammetric responses on 1:1

288 These pI values measured with the developed method were close

289 mutants were also demonstrated to migrate to pI values consistent with predictions (pI 7.0 for one de

290 observed post-translational modifications to pI shifts relative to the unmodified protein of interest

291 l sequence-based characteristics relating to pI, hydrophobicity, Ser abundance, and Phe bilayer asymm

292 More recently, an off-line method that uses pI-based separation on immobilized pH gradients was deve

293 ed VCP expression and an acidic shift in VCP pI, which was inhibited by protein phosphatase 2A treatm

294 analysis of other acidic polypeptides, whose pI< or =4.

295 entified four human brain SOD1 isoforms with pI values of 6.3, 6.0, 5.7, and 5.0, respectively.

296 longs to the class of neutral proteases with pI value of 6.8, optimal proteolytic activity displayed

297 ization, and identification of proteins with pI values greater than 7.

298 , a second fraction containing proteins with pI values in the same range as the pI of albumin, and a

299 nd a third fraction containing proteins with pI values lower than the pI of albumin.

300 When used with pI-based fractionation as a first dimension, it provides