ライフサイエンスコーパス: pancreata

1 stine, 40 kidney, 20 livers, 24 lungs, and 7 pancreata).

2 ing of embryonic day (e) 13.5 and 15.5 mouse pancreata.

3 al neoplasias and PDAs compared with healthy pancreata.

4 yses of the IkappaBalpha- and RelA-deficient pancreata.

5 neutrophil recruitment compared with normal pancreata.

6 antities of insulin were present in most T1D pancreata.

7 alpha-cells in Gcgr(-/-) and Gcgr(Hep)(-/-) pancreata.

8 istologic examination of salivary glands and pancreata.

9 is is induced in postpartum FoxM1(Deltapanc) pancreata.

10 tained primary cilia in both human and mouse pancreata.

11 ding outpouches of ducts in murine and human pancreata.

12 the native insulin B:9-23 sequence in their pancreata.

13 +/- 3.0%) and obese diabetic (18.5 +/- 3.6%) pancreata.

14 tion of PGC-GdDTPA-F in diabetic and control pancreata.

15 P < 0.01) in obese diabetic (0.49 +/- 0.17%) pancreata.

16 rine embryonic stem cells to early endocrine pancreata.

17 n secretion compared to islets from SCS only pancreata.

18 man islets were obtained from research-grade pancreata.

19 ic islets of Langerhans of adult human donor pancreata.

20 et, very few beta-cells persist within their pancreata.

21 ation is an insufficient supply of cadaveric pancreata.

22 fected pancreata but not in CVB3/GA-infected pancreata.

23 ext, we analyzed the oxidative stress in the pancreata.

24 target, was also activated in the Ucp2(-/-) pancreata.

25 glucagon was similar to that from wild-type pancreata.

26 mes of VXM after transplantation of imported pancreata.

27 let development in Neurog3-null mutant mouse pancreata.

28 rgans included 1,779 kidneys, 395 livers, 54 pancreata, 2 lungs, and 1 heart.

29 Imported pancreata accumulate cold ischemia time (CIT), limiting

30 atic Organ Donors With Diabetes (nPOD) human pancreata across a wide range of ages and T1D disease du

31 tic acinar tissue and from fibrous stroma in pancreata affected by chronic pancreatitis.

32 d on studies of human, mouse, and guinea pig pancreata, alcohol disrupts expression and localization

33 long-term high fat diet (HFD), M4K4 iKO mice pancreata also displayed reduced beta cell mass, fewer p

34 revealed mosaic MANF expression in postnatal pancreata and a significant correlation between the numb

35 The ductal system was analyzed in normal pancreata and chronic pancreatitis in humans and mice.

36 Mouse pancreata and clonal beta-cells were examined for expres

37 acceptance criteria for deceased donor organ pancreata and critical raw materials, PHPI product speci

38 Studies of human pancreata and cultured islets have shown significant var

39 ation with infusions from two to three donor pancreata and Edmonton immunosuppression consistently ac

40 xpression was comparable with liver in mouse pancreata and in rat and human islets.

41 ple experimental systems, including perfused pancreata and isolated islets of rodent or human origin.

42 Pancreata and liver tissues were collected and analyzed

43 Pancreata and lung tissues from mice were analyzed by hi

44 Pancreata and lungs were harvested, and histologic secti

45 ssfully and consistently produced from human pancreata and maintain their innate molecular and spatia

46 We isolated islets from 10 human pancreata and measured the expression of Bax mRNA and Bc

47 Numerous studies of isolated pancreata and pancreatic islets support the hypothesis t

48 Pancreata and primary acinar cells were isolated; acinar

49 proves contrast between healthy and diabetic pancreata and provides a potential avenue for achieving

50 llected from C57BL/6 mice and resected human pancreata and were cultured.

51 om three independent hPSC lines, human fetal pancreata, and adult human islets points to two major co

52 s on patient safety and care, prudent use of pancreata, and consolidates PII expertise and experience

53 As were cloned and sequenced from developing pancreata, and expression of a subset of these genes was

54 pproaches, organotypic cultures of embryonic pancreata, and genomics, we found that sphingosine-1-pho

55 as greatly accelerated in Mist1KO/KrasG12D/+ pancreata, and in vitro assays revealed that Mist1KO aci

56 ot used are smaller than numbers of kidneys, pancreata, and livers because intestines, hearts, and lu

57 -fixed paraffin-embedded surgically resected pancreata, and were analyzed by methylation-specific pol

58 crine congenital defects of Prox1(DeltaPanc) pancreata appeared to initiate a gradual process of dete

59 ons at low frequency, and in most cases, the pancreata are histologically normal.

60 ween 3.5 and 6.2 in otherwise transplantable pancreata are not associated with different short-term o

61 ese data also suggest that many good quality pancreata are not procured, emphasizing the need for imp

62 After donor cross-clamp in Houston, donor pancreata arrived at DRI and the isolation process began

63 ing was performed on wild-type and MafB(-/-) pancreata at embryonic day 18.5, with candidates evaluat

64 In contrast, the PKC pancreata bearing a Grp78(f/+) allele (PKC78(f/+) mice)

65 The PKC pancreata bearing wild-type Grp78 showed detectable PDAC

66 extend the acceptable preservation period of pancreata before islet isolation and increase islet yiel

67 pothesis that such changes are present in CF pancreata before the development of lipofibrosis.

68 nick end labeling) assay in CVB3/M-infected pancreata but not in CVB3/GA-infected pancreata.

69 both mouse embryonic and adult regenerating pancreata but was absent in normal adult islets.

70 cells are specified normally in Hox6 mutant pancreata, but fail to mature into hormone-producing cel

71 , and delta-cells from human fetal and adult pancreata by intracellular staining for the cell-specifi

72 We have previously shown that rat pancreata can be created from rat pluripotent stem cells

73 etion or heterozygous loss of Bap1 in murine pancreata causes genomic instability, tissue damage, and

74 CPV/49 replicates in murine hearts and pancreata, causing no disease in hearts and a minor panc

75 HEV in wild-type LN and in RIPLT alpha beta pancreata, coincident with HEC-6ST.

76 ient rat blastocysts, we generated rat-sized pancreata composed of mouse-PSC-derived cells.

77 Pancreata developing without beta-catenin are hypoplasti

78 le Gata4/Gata6 mutant mice failed to develop pancreata, died shortly after birth, and displayed hyper

79 histologically similar to WT, whereas K8/K18 pancreata displayed age-enhanced vacuolization and atrop

80 Interestingly, type 1 diabetes pancreata displayed significant reductions in the freque

81 ss was normal in postpartum FoxM1(Deltapanc) pancreata due to a combination of increased beta-cell si

82 ir and resuscitate warm ischemically injured pancreata during preservation, improve pancreas graft su

83 te endocrine progenitor cells from embryonic pancreata (embryonic day [E]13.5 through E17.5).

84 F-alpha, MIP-1alpha, IL-12, and IFN-gamma in pancreata, endotoxin, or anti-CD3-stimulated splenic cel

85 CFRD pancreata exhibit reduced islet density and altered cell

86 CFRD pancreata exhibited greater islet injury with further re

87 RIPLT alpha beta pancreata exhibited massive intra-islet mononuclear infi

88 Pancreata exposed to long CIT yielded lower islet number

89 PDGs and PanIN lesions in rodent and human pancreata express the GLP-1 receptor.

90 gram (HPAP) to procure human type 1 diabetes pancreata for an extensive array of tissue-based, cellul

91 valent to UW solution in the preservation of pancreata for islet isolation.

92 on and could provide a significant source of pancreata for islet transplantation.

93 Pancreata from 1- to 3-day-old pigs were collagenase dig

94 Pancreata from 2- to 4-month-old mice were dissociated,

95 Examination of pancreata from age-matched organ donors with type 2 diab

96 ive (AMY(+)) acinar cells were detectable in pancreata from all study groups, tissues from individual

97 Furthermore, pancreata from animals treated with 10 mg/kg BRD3308 exh

98 Pancreata from ATG5-deficient mice had signs of inflamma

99 chronic pancreatitis (CP) and compared with pancreata from ATG5-deficient mice.

100 lipase, and/or trypsinogen in 78 organ donor pancreata from birth through adulthood in control subjec

101 3) Do recipients of whole pancreata from cadaveric donors have twice the amount of

102 Levels of BCL3 were higher in pancreata from control mice given cerulein than from mic

103 es, and the histopathological examination of pancreata from diabetic organ donors with the goal of pr

104 A substantial nationwide underutilization of pancreata from donor procurements is demonstrated in the

105 adopted a new allocation algorithm in which pancreata from donors >30 kg/m or >50 years of age are,

106 ficity for type 2 diabetes, we also examined pancreata from eight cases of type 1 diabetes.

107 Pancreata from extended criteria donors were flushed and

108 Furthermore, pancreata from MafB deficient (kr(ENU)/kr(ENU)) mice exh

109 Postmortem analysis of pancreata from Medalists revealed the presence of insuli

110 Pancreata from mice exposed to radiation had fewer CD8(+

111 xpression of hepatic markers was examined in pancreata from mice genetically modified to secrete elev

112 Pancreata from mice, rats, and nonhuman primates were ex

113 These data support that pancreata from mildly obese donors (BMI, 30-35 kg/m) can

114 onist enhanced insulin secretion in perfused pancreata from neonatal streptozotocin-induced diabetic

115 Here, we demonstrate that pancreata from NOD mice with islet infiltration have enh

116 Pancreata from NOD-gld/+ mice contained B cells that exp

117 f preneoplastic proliferative lesions in the pancreata from nonhuman primates was performed.

118 Consideration of pancreata from obese donors may decrease the pancreas di

119 Pancreata from older and more obese donors were used mor

120 udies in human acini isolated from cadaveric pancreata from organ donors.

121 Finally, examination of human pancreata from patients with T1D revealed that VEGFR-2 w

122 lopment of beta-cells, we analyzed embryonic pancreata from Pax6- and MafB-deficient mice.

123 In contrast, pancreata from pdx-1(-/-) mice expressing the mutant PDX

124 Pancreata from Ptf1a-Cre(ERTM);LSL-Kras(G12D) mice conta

125 Pancreata from Ptf1a-Cre(ERTM);LSL-Kras(G12D);Klf5(fl/fl

126 Since recent studies on human pancreata from T1D patients have indicated that both lig

127 recise tissue distribution of CD8 T cells in pancreata from T1D, T2D, autoantibody-positive, and heal

128 roximately 30% decrease in beta cell mass in pancreata from Tcfl2(fl/fl)::Ins1Cre mice.

129 in or sodium taurocholate than control mice; pancreata from the Bcl3(-/-) mice with AP had greater nu

130 Pancreata from these mice had reduced autophagy, increas

131 Pancreata from transgenic mice display early-onset acina

132 Results: In KPC mouse pancreata, gammaH2AX expression was increased in high-gr

133 Results: In KPC mouse pancreata, gammaH2AX expression was increased in high-gr

134 Thus, in exocrine pancreata, Grp78 heterozygosity regulates ER chaperone b

135 ntitative RT-PCR showed that the Pax6(PE/PE) pancreata had a significant decrease in Pax6, glucagon,

136 More than 50% of recovered pancreata had at least one injury, most commonly a short

137 Persufflated pancreata had reduced SCS time, which resulted in islets

138 Ninety-seven percent of pancreata had three lobes: duodenal lobe (DL), connectin

139 lpha-specific gene expression, and Grg3(+/-) pancreata have more alpha-cells and more polyhormonal ce

140 -term functional graft survival data for DCD pancreata have not been reported.

141 Numerous pancreata have not been used because of long ischemia ti

142 RNA sequencing of islets from persufflated pancreata identified reduced inflammatory and greater me

143 elded significantly more islets than control pancreata (IEQ/g: 2134+/-297 vs. 1477+/-145 IEQ/g or 65,

144 For pancreata imported from United Network of Organ Sharing

145 Pancreata in DM treated with incretin therapy were notab

146 turation and might be used to repair damaged pancreata in patients with pancreatic disorders.

147 e disposition and outcomes of deceased donor pancreata in the United States between January 1, 2000 a

148 The pancreata in wild-type (WT) mice (n = 64) were scanned a

149 , insulin-producing cell lines, and perfused pancreata indicated xenin-25 does not enhance GIP-mediat

150 ed with NKX6-1 and PTF1A in human developing pancreata, indicating that it marks the multipotent panc

151 ic ghrelin-producing cells are maintained in pancreata lacking the essential endocrine lineage regula

152 ficiency in both the PKC78(f/+) and c78(f/+) pancreata leads to reduction of epidermal growth factor

153 proach are an inadequate supply of cadaveric pancreata, lifelong immunosuppression, and chronic graft

154 p38IH treatment of human pancreata may improve islet yield for use in clinical tr

155 dichotomy raises the hypothesis that K8-null pancreata may undergo compensatory cytoprotective gene e

156 measured via immunohistochemical analysis of pancreata measuring loss of acini, fibrosis, inflammatio

157 ceased donor (n=30) and chronic pancreatitis pancreata (n=30).

158 ived cells were observed in the nonendocrine pancreata, no pancreatic beta-cells were identified that

159 We addressed this in 31 human pancreata obtained at autopsy from hematopoietic stem ce

160 diabetic, and 14 obese type 2 diabetic human pancreata obtained at autopsy.

161 Concerns exist regarding the utilization of pancreata obtained from DCD donors.

162 we observed no abnormal pathology within the pancreata of 23 Pdx1-Cre;Smad4(lox/lox) animals that wer

163 Islet size and insulin content in pancreata of A2AAR-deficient mice were decreased compare

164 ls and profiled more than 120,000 cells from pancreata of adult and neonatal human donors.

165 Pancreata of Bmi1(-/-) mice were hypoplastic, and the ex

166 f the CCR2-ligand (CCL2) was observed in the pancreata of cadaveric donors, suggesting that beta-cell

167 In contrast, the pancreata of CD1d-null mice harbored significantly highe

168 y qRT-PCR using RNA derived from spleens and pancreata of cured mice validated the suppression of mos

169 peri-insulitic infiltrate were found in the pancreata of cured NOD mice.

170 greater accumulation of PGC-GdDTPA-F in the pancreata of diabetic animals compared with controls.

171 Previously, pancreata of dying alcoholics and pancreatic necrosis in

172 Pancreata of elastase sshIL-1beta mice were analyzed for

173 rx(+) cells was significantly reduced in the pancreata of embryos deficient for the Islet-1 (Isl-1) t

174 diated insulin release from in situ perfused pancreata of GIP/DT mice.

175 re significantly decreased in the developing pancreata of Glis3(zf/zf) mutant mice.

176 Pancreata of KC mice exposed to radiation had a higher f

177 i, we electroporated oncogenic plasmids into pancreata of LSL-KrasG12D x p53fl/fl mice; mutant Kras w

178 We disrupted Acvr1b specifically in pancreata of mice (Acvr1b(flox/flox);Pdx1-Cre mice) and

179 NETs form in the pancreata of mice during the development of AP, and NET

180 Loss of ACVR1B from pancreata of mice increased the proliferation of pancrea

181 verse relaxation time (T2) shortening in the pancreata of mice injected with the MN-Ex10-Cy5.5 probe

182 n of an activated form of KRAS (KrasG12D) in pancreata of mice is sufficient to induce formation of p

183 gnificant up-regulation of the Hh pathway in pancreata of mice overexpressing GLI2.

184 plex II(+)CD11c(+) DCs increased 100-fold in pancreata of mice with acute pancreatitis to account for

185 Pancreata of mice with Sbds mutations had decreased mass

186 Defects developed specifically in the pancreata of mice, reducing growth of mice and productio

187 contrast-induced activation of NF-kappaB in pancreata of mice, this was observed by live imaging of

188 alpha (eIF-2 alpha[P]) levels in nonstressed pancreata of mice.

189 Although the pancreata of Mist1(KrasG12D/+) mutant mice predictably e

190 imulated insulin secretion from the perfused pancreata of MKR mice and reduced the beta-cell mass.

191 The pancreata of Mmp9(-/-) mice did not differ from wild typ

192 leakage, as an indicator of inflammation, in pancreata of mouse models of type 1 diabetes ex vivo or

193 educed immune infiltration and activation in pancreata of NOD mice as well as humanized NOD Scid IL2

194 ivation, while increasing Treg activation in pancreata of NOD mice.

195 scaffolds that were also expanded within the pancreata of NOD mice.

196 4 and IL-10 were significantly higher in the pancreata of OGG-1(-/-) mice as compared with the levels

197 Pancreata of Pdx-Shh mice (in which Shh is misexpressed

198 In the pancreata of Pdx1-Cre; LSL-Kras(G12D) mice, exendin-4 le

199 Pancreata of Perk(-/-) mice are morphologically and func

200 ouse homologues of HIP/PAP was determined in pancreata of prediabetic and diabetic NOD mice.

201 Furthermore, DeltaT2 of the pancreata of prediabetic NOD mice was significantly high

202 Exocrine pancreata of RD-fed Grp78(+/-) mice in an outbred C57BL/

203 e mainly found in pancreatic lymph nodes and pancreata of recipients, indicating that the transferred

204 eased islet cell number and proliferation in pancreata of rPdx1-treated mice.

205 entage of polyhormonal cells in the neonatal pancreata of Snord116p-/m+ mice, due primarily to an inc

206 Histological examination of the pancreata of the control mice revealed a severe and dest

207 ed PDX1 rescues the Pdx1-null phenotype; the pancreata of these mice develop and function normally.

208 In pancreata of transgenic mice, marked hyperplasia of cell

209 c periductal lymphocytic infiltration in the pancreata of transgenic mice, predominantly CD4+ T lymph

210 as IFN-gamma production was increased in the pancreata of treated animals, insulin expression was str

211 The pancreata of treated mice showed significantly lower lev

212 c patients directly, we isolated islets from pancreata of type 2 diabetic cadaveric donors (n = 14) a

213 esions and more foci of invasive cancer than pancreata of unexposed mice (controls); radiation reduce

214 by comparing the gene expression profile in pancreata of wild-type and K8-null mice.

215 ransgenic mice compared with normal, control pancreata of WT mice (mean intensity, 0.25 lau +/- 0.25)

216 - and beta-cell subsets from fetal and adult pancreata offers clear implications for strategies that

217 ed at any of the 15 CpG islands in 15 normal pancreata or in an immortalized normal pancreatic duct e

218 number of islets of Langerhans (two to four pancreata per recipient) into patients with type I diabe

219 8.8%) kidneys, 3/5(60%) livers and 1/2 (50%) pancreata performed.

220 In type 1 diabetic pancreata, perinuclear CHOP was rare (2.5 +/- 2.3%) and

221 lls detected in heterozygous and hypomorphic pancreata, possibly those that express low levels of Neu

222 Pancreata preserved in the TLM are oxygenated through th

223 ess-than-ideal donors, the ratio of liver to pancreata procured can be reduced to 1.25:1.

224 Islets were isolated from pancreata procured from 12 cadaveric organ donors and cu

225 All pancreatic islets were isolated from pancreata procured in Houston and subsequently transport

226 scard rate in 2011 was 27.7%, and higher for pancreata recovered from older donors.

227 btypes, and beta-cell and alpha-cell mass in pancreata recovered from organ donors with type 1 diabet

228 Pancreata removed from Beagle dogs were infused with Uni

229 cent analyses of transgenic rodent and human pancreata reveal a number of novel heterogeneity markers

230 Histologic analyses of RARdn-expressing pancreata revealed a decrease in beta-cell mass and insu

231 We found that perfused Sur1 null pancreata secreted insulin in response to the cholinergi

232 peri-islet cell accumulations in RIPLT alpha pancreata: separation into T and B cell areas was more d

233 Adult DKO pancreata showed no histological abnormalities, and immu

234 Compared with PKC, the PKC78(f/+) pancreata showed substantial reduction of ADM as well as

235 observations, immunohistological analysis of pancreata showed that CD105(+) cells are restricted to t

236 This allows pancreata stored in the TLM to generate adenosine tripho

237 techniques for isolating islets require that pancreata stored with University of Wisconsin solution (

238 negative constructs and isolated human fetal pancreata suggest that c-Src is at least partially respo

239 /progenitor cells, prepared from human adult pancreata survive engraftment and produce tissue chimeri

240 Vessel density was less in IUGR pancreata than in controls.

241 Because donor pancreata that are unsuitable for WOP can often be used

242 Of whole organ pancreata that were transplanted, 90% were from donors w

243 y distinct from normal ducts; even in normal pancreata they display PanIN and metaplastic features, s

244 t isolation and reduce the need for multiple pancreata to achieve insulin independence in the recipie

245 ith placental defects, so we explanted E12.5 pancreata to allow endocrine differentiation to occur in

246 or influencing islet function is exposure of pancreata to cold ischemia during unavoidable windows of

247 ison with UW organ preservation, exposure of pancreata to the TLM result in greater islet yields and

248 We analyzed the ratio of livers to pancreata transplanted at our center and compared this t

249 ignaling was inhibited in cultured embryonic pancreata using morpholine-ring antisense against GIP li

250 Collagenase digestion of pancreata was carried out using standard procedures.

251 ration index of acinar cells from Mist1 null pancreata was evaluated by immunohistochemistry.

252 al recovered islet mass from type 2 diabetic pancreata was significantly less than that from nondiabe

253 tablish the functional role of PERK in adult pancreata, we generated mice harboring a conditional PER

254 s of thousands of cells in human organ donor pancreata, we show that quantitative graph characteristi

255 Zymogen granules in K8/K18 pancreata were 50% smaller and more dispersed than their

256 ross and histologic findings in the resected pancreata were also assessed.

257 Pancreata were analyzed by histology and immunohistochem

258 Pancreata were analyzed by terminal deoxynucleotide tran

259 Morphological defects in Gata4/Gata6 mutant pancreata were apparent during embryonic development, an

260 Pancreata were collected and analyzed by histology, immu

261 Pancreata were collected and analyzed by immunohistochem

262 Pancreata were collected and extracellular DNA was detec

263 Pancreata were collected and histologically analyzed for

264 Pancreata were collected and histology, immunohistochemi

265 Pancreata were collected at different stages of tumor de

266 Pancreata were collected from mice and analyzed by histo

267 Pancreata were collected from mice and analyzed for Kras

268 Pancreata were collected when mice were 28 weeks to 14.5

269 To achieve our goal, human pancreata were decellularized with Triton-based solution

270 or resveratrol (HFS+Resv) for 24 months, and pancreata were examined before overt dysglycemia occurre

271 Pancreata were exposed to doses of radiation ranging fro

272 Islets obtained from long-CIT pancreata were functionally impaired after transplantati

273 beta1 Integrin-null pancreata were highly susceptible to cerulein-induced ac

274 K8- and K18-overexpressing pancreata were histologically similar to WT, whereas K8/

275 Four pancreata were maintained as a control group in UW alone

276 In the second experimental set, six pancreata were maintained for an average of 6.4+/-1.8 ho

277 In the first experimental set, eight pancreata were maintained for an average of 8.3+/-1.2 ho

278 Degenerating beta1 integrin-null pancreata were marked by disruption of acinar cell polar

279 composed of rat-derived cells, the resulting pancreata were of mouse size, rendering them insufficien

280 Human pancreata were preserved by SCS or by persufflation in c

281 tween January 16, 2002 and June 30, 2003, 39 pancreata were procured and processed for PIT at a dista

282 All pancreata were procured by a single surgeon, and special

283 Twelve hundred ninety-six pancreata were procured from donation after brain death

284 Pancreata were removed by en bloc viscerectomy from 65 f

285 Rat pancreata were stored in cold University of Wisconsin pr

286 ntly prepared from these mouse-rat chimaeric pancreata were transplanted into mice with streptozotoci

287 When pancreata were transplanted under the kidney capsules of

288 15 patients with PDAC; histologically normal pancreata were used as controls (n = 18).

289 6,000 organ donors, only approximately 1,500 pancreata were used for clinical transplantation.

290 ntial donors during this period, 6,140 donor pancreata were used for whole organ transplant.

291 insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta

292 This damage may be prevented by treating pancreata with a p38MAPK inhibitor (p38IH) before cold p

293 raft loss was significantly more frequent in pancreata with arterial damage (p = 0.04) and in those w

294 underwent single-pass perfusion of isolated pancreata with low and high glucose concentration (n = 4

295 Infusion of pancreata with University of Wisconsin solution containi

296 t NKG2D and its ligands are present in human pancreata, with expression of NKG2D and its ligands incr

297 -16 mice developed dedifferentiated exocrine pancreata within 2 months of age and became malnourished

298 All but one of the PanINs from the 14 pancreata without pancreatic carcinoma was unmethylated

299 Twenty-six of 39 (67%) procured pancreata yielded more tha 5,000 islet equivalents (IEQ)

300 p38IH-treated pancreata yielded significantly more islets than control