ライフサイエンスコーパス: panning

1 hCD20-specific antibodies in four rounds of panning.

2 ive antigens after as few as three rounds of panning.

3 purified from FVB/N mouse stomachs by lectin panning.

4 t sufficiently high to allow purification by panning.

5 raries of rANP-display phage by differential panning.

6 d a 195-fold enrichment after five cycles of panning.

7 minant cells into the supernatant, like gold panning.

8 , was more than 100-fold increased after the panning.

9 ide Y, following screening by solution phage panning.

10 imized by using phage display and cell-based panning.

11 specificity of the interaction identified by panning.

12 After three rounds of subtractive panning, 239 of 673 clones analyzed bound selectively to

13 Nipah virus (NiV) and Hendra virus (HeV) by panning a large nonimmune antibody library against a sol

14 anti-Akt1 single-chain antibodies (scFv) by panning a mouse phage-displayed scFv recombinant antibod

15 fied a TREM2 agonist monoclonal Ab (Ab18) by panning a phage-displayed single-chain variable fragment

16 isplay format and make it ideally suited for panning a wide variety of target antigens.

17 From this panning, a C-terminal fragment of FGF-BP1 (amino acids 1

18 r muscle-targeting capsids by direct in vivo panning after tail vein injection in mice.

19 ainst fresh neutrophils and independently by panning against a fixed cell line.

20 Panning against bovine trypsin resulted in enrichment of

21 Phage were selected by panning against digoxin, gitoxin (16-OH), and 16-acetylg

22 able fragments (scFvs) after three rounds of panning against FAP.

23 high-affinity human mAb, ANA15, selected by panning against fresh neutrophils and independently by p

24 ide phage display libraries using whole-cell panning against human melanoma cell line Me6652/4.

25 eased iRBC cytoadhesion activity by repeated panning against human umbilical vein endothelial cells (

26 (scFv) phage-display library was used in bio-panning against live, native spores of Bacillus subtilis

27 s from peptide-presenting phage libraries by panning against primary patient CLL cancer cells.

28 Panning against rat trypsin resulted in enrichment as we

29 Panning against rat trypsin resulted in enrichment but n

30 In contrast, panning against secreted EBO glycoprotein (SGP) resulted

31 nesis of its heavy-chain variable domain and panning against sGHeV.

32 0 cDNA-bacteriophage library was enriched by panning against solid- and solution-phase PAI-1.

33 cted from a human nonimmune phage library by panning against the chimeric construct N(CCG)-gp41 (whic

34 age-displayed Fab, scFv, and VH libraries by panning against the receptor binding domain (RBD) of the

35 specific cPTMs are significantly enriched in panning against the specific targets.

36 ry binding site of ecotin, was generated for panning against uPA and rat trypsin.

37 Panning against uPA resulted in enrichment as well as th

38 methodological innovation, termed iterative panning and blocking (IPAB), to extend the range of spec

39 the surface of 1264 and BEAS cells used for panning and counter-selection was estimated as 75,000 +/

40 A direct in vitro panning and enzyme-linked immunosorbent assay-based sele

41 d signaling networks with dynamical zooming, panning and linking capabilities.

42 derpin this diverse functionality, molecular panning and mass spectrometry are used to identify prote

43 s that could be missed by conventional phage panning and screening methods.

44 y bound or eluted phage at various stages of panning and screening procedures.

45 We use a solution-based panning and screening strategy conducted in the presence

46 Repeated panning and selection experiments against a 15-mer pepti

47 ystin LR and its selection using competitive panning and two novel panning strategies.

48 ionary dual-view imaging device with optical panning and zooming capabilities.

49 SurfaceSlide users can perform direct panning and zooming operations on digitised slide images

50 was provisionally mapped by peptide display panning, and a peptide sequence matching the epitope was

51 addition, offer the flexibility of zooming, panning, and being adjustable for slow image acquisition

52 lly support Tat transactivation was used for panning, and of the five unique anti-hCyclinT1 sFvs that

53 Here we applied an in vitro whole cell panning approach using antibody phage display technology

54 Through a whole-cell panning approach, we previously identified a panel of an

55 By this "epitope-blocked panning" approach, two novel Fabs, encoded by unique VH

56 an efficiency that exceeds surface-confined panning approaches; and (3) the co-detection of ssDNAs,

57 rovide a intuitive interface for zooming and panning around digital images.

58 and function of CHIP, we report an antibody panning campaign that yielded six recombinant Fabs with

59 Optical zooming and panning capabilities facilitate repositioning of the zoo

60 Thus, phage library panning combined with expression cloning permits identif

61 VVYV) from phage display libraries using six panning conditions.

62 selection by tailoring the stringency of the panning conditions.

63 Here, phage-peptide library panning coupled with screening using next generation seq

64 the antigenic profile of the cells used for panning determines the specificity of the preponderant p

65 ith a streamlined strategy for phage display panning enable the rapid isolation and identification of

66 Incorporating in silico cross-panning enabled computational prescreening of specificit

67 Phage recovered from this second panning exhibited a motif (Ser-His-Tyr) that was subsequ

68 Sequencing data of the phage panning experiment are deposited at NIH's Sequence Read

69 Using M13qPCR in phage-panning experiments on live leukemia and prostate cancer

70 ior to candidates derived from phage display panning experiments.

71 lection of this peptide in multiple reported panning experiments.

72 kat libraries enabled repeated screening and panning for antigen-reactive TCRs using peptide major hi

73 (NAR) s that bind to the S2 subunit by phage panning from a naive nurse shark V(NAR) phage display li

74 ns to be propagated as either phage for more panning from repertoires or expressed as soluble sdAb fo

75 ptides, Bicycle, inhibitors of human ACE2 by panning highly diverse bacteriophage display libraries.

76 A panning in microtiter plates resulted in 22 unique in vi

77 nique in vitro neutralizing antibodies and a panning in solution combined with a functional neutraliz

78 After five rounds of panning, individual positive scFv clones were used to in

79 ii) selected functional recombinant phage by panning; (iv) purified the ScFv peptide; (v) shown that

80 ociated retinal cells by a modified two-step panning method and were cultured in serum-free medium co

81 Here, we use a novel cysteine panning method to identify the second cleavage site (Sit

82 ostnatal days 7 and 8 by a modified two-step panning method.

83 may not be accessible by conventional B cell panning methods.

84 through a phage display library and by (ii) panning multiple libraries encoding structurally distinc

85 phage-displayed heavy chain-only antibody by panning of a large (size, approximately 1.5x10(10)) huma

86 dra G (sG) which was used as the antigen for panning of a large naive human antibody library.

87 Affinity panning of a library of humanized A4.6.1 antibody mutant

88 dy FC5 was previously isolated by phenotypic panning of a naive llama single-domain antibody phage di

89 To this end, we utilized a sequential panning of a phage library on cultured rat EpiSCs and th

90 in soluble CD4 (sCD4) were alternated during panning of a phage-displayed human antibody library; the

91 G(M3)-derived reagents and their use in the panning of a phage-displayed human single-chain Fv (scFv

92 tence of such a motif was established by the panning of a random peptide library in which peptide seq

93 ssive molecule CD200 were identified by cell panning of an antibody phage display library derived fro

94 Thus, direct in vivo panning of capsid libraries is a simple tool for the de-

95 Panning of IEs on HBMECs led to a more dispersed binding

96 motifs that bind to GroEL by using affinity panning of immobilised GroEL minichaperones for a librar

97 er of structure-based design with sequential panning of large D1 mutant libraries against different H

98 Affinity panning of large libraries is a powerful tool to identif

99 Panning of phage display peptide libraries with mAb 763.

100 Panning of phage Fab libraries against purified antigens

101 regions of arrestin that bind to rhodopsin: panning of phage-displayed arrestin fragments against RP

102 Panning of such libraries enriched strongly peptides wit

103 Panning of the hot spot libraries yielded several mutant

104 Initial panning of the library isolated only non-pathogenic scFv

105 1.7 x 10(4) sequences obtained from repeated panning of the library.

106 Affinity panning of these resulted in the selection of variants w

107 patients to receive phage library for serial panning of tumor targeting ligands.

108 n (within 6 d of availability of antigen for panning) of potent mAbs shows the value of large antibod

109 ingle-chain Fv antibody fragments (ScFvs) by panning on a prothrombin-coated surface.

110 By panning on B chain, we identified binders and have chara

111 Panning on carbonic anhydrase yielded a potent ligand, s

112 antibodies directly from phage libraries by panning on cells.

113 ffinities for digoxin increased 2-4-fold, by panning on digoxin-BSA yet retaining the specificity shi

114 approach involving preclearing steps before panning on HUVECs, we isolated and sequenced 140 individ

115 After three rounds of panning on recombinant mesothelin, a single-chain Fv (sc

116 on of profiles the phage library enriched by panning on the pool of cancer sera.

117 -specific T cells from animals with SGVHD by panning or by flow cytometric sorting.

118 odologies that rely on microdissection, cell panning or cell sorting, the TRAP methodology bypasses t

119 Panning peptide phage display libraries led to the ident

120 A third round of panning performed in the presence of both the fibrinogen

121 trate, S-adenosylmethionine (AdoMet), during panning permitted PIMT to retain aged phage in greater n

122 The CERVIGTGWVRC sequence was identified by panning phage display libraries on the inhibitory CD47 m

123 tended to specific application of M13qPCR in panning phage libraries on tissue sections of prostate a

124 e sera were then enriched using a multi-step panning procedure.

125 riments performed on this library during the panning process, this scFv bound 1200-fold less tightly

126 ealed by blockade of this epitope during the panning process.

127 es a number of aspects of conventional phage-panning protocols.

128 sequences that reacted specifically to their panning rAb by enzyme-linked immunosorbent assay.

129 we have demonstrated that a synthetic G(M3) panning reagent can be used to isolate a fully human scF

130 The initial panning recovered phage that bore the consensus motif Gl

131 However, panning rounds are followed by the tedious re-screening

132 body binding phages was observed after three panning rounds, and sequence analysis of randomly select

133 ycoproteins (Envs) dubbed sequential antigen panning (SAP).

134 on of binders to conserved Env structures by panning sequentially against Envs from different isolate

135 Six phage clones identified from S-20-4 panning showed significant binding to both S-20-4 and A-

136 to identify drivers of vascular invasion by panning small hairpin RNA (shRNA) library-transduced non

137 hlight the importance of exploring different panning strategies to optimize the selection of antihapt

138 against DT from two different phage display panning strategies using a human immune library.

139 om a panel of 11 Fabs recovered by different panning strategies, three highly potent neutralizing ant

140 tion using competitive panning and two novel panning strategies.

141 We utilised a novel phage display panning strategy to isolate a high affinity pool (KD ran

142 e positive clones and by using a subtractive panning strategy to remove crossreactivity with B. liche

143 ntigen receptors (PC-CARs) through a counter panning strategy using predicted potentially cross-react

144 ric antigen receptors (CARs) using a counter-panning strategy with predicted potentially cross-reacti

145 rized nonimmune human Ab-phage library and a panning strategy with proteoliposomes and cells to ident

146 play antibody library by using an exhaustive-panning strategy.

147 en, and failure of clonal enrichment by cell panning suggested that the neoantigen was not membrane e

148 of the scFv fragments that were isolated by panning synthetic Ab libraries with different melanoma c

149 the variable region (scFvs) were isolated by panning synthetic scFv library 1 on purified HMW-MAA.

150 This was the first report of a modified panning technique to isolate adult pig RGCs.

151 ated from other retinal tissue by a modified panning technique using Thy 1.1 antibody.

152 The antigens used for panning the antibodies comprised two soluble, disulfide-

153 cFv library of the melanoma patient and then panning the libraries against melanoma cells to enrich f

154 ce homologies between peptides obtained from panning the library against the antibodies and the nativ

155 ice, measuring Ab responses to immunization, panning the phage library, high-throughput screening of

156 fragment of the V region (scFv) fragments by panning the synthetic phage Ab library (#1) with the hum

157 n any detectable MV Ags; after six rounds of panning, the positive clones comprised 34% of all phage

158 Panning this library against PAb1620 resulted in three u

159 in Java that supports real-time zooming and panning through a genome; layout of genomic features and

160 bs, a representative Fab was included during panning to block this common epitope on the F Ag.

161 By applying phage display panning to hundreds of bacterial genotypes and analyzing

162 We screened a phage library in vivo by panning to identify peptides that bound the vascular end

163 Subsequent panning trials using this library yielded promising hits

164 n of VHHs recognizing BDE-47 was achieved by panning under carefully modified conditions.

165 stable C(H)3 heterodimers were recovered by panning using an anti-flag antibody.

166 A second round of panning was performed against the same target mixture in

167 ner(s) for sperm acrosomal ligands, affinity panning was performed with mouse oocyte lysates using sp

168 In three patients, repeat panning was possible using phage recovered and amplified

169 rified by counterflow elutriation and lectin panning, was validated by real-time quantitative reverse

170 Using phage panning we have also identified several short peptide se

171 wing rounds of selection and deep mutational panning, we identified a high-fidelity peptide consensus

172 ens from immunized mice and in vitro library panning, we identified two antibodies, 2A4 and 1G12.2A4

173 The tool offers smooth zooming and panning which is more flexible than seen in other browse

174 d to search for tumor-associated antigens by panning with a lung adenocarcinoma cell line, 1264, and

175 Yeast panning with a nonimmune human single-chain antibody lib

176 Panning with an allogeneic breast cancer cell line enric

177 population in the spleens enriched either by panning with anti-guinea pig immunoglobulin G-coated pla

178 However, panning with autologous tumor tissue lysate increased bi

179 By panning with DNA, the SLE libraries each yielded IgG ant

180 Selection of phage by panning with fixed neutrophils yielded a 195-fold enrich

181 from 7- to 15mer phage display libraries by panning with hyaluronan-Sepharose beads and assayed for

182 elected from randomly generated libraries by panning with recombinant SCR1-2, followed by specific li

183 ated from phage display peptide libraries by panning with self-tumor-associated Ag (TAA)-specific mAb

184 r EGFRvIII was isolated from this library by panning with successively decreasing amounts of syntheti

185 quid cultures 24 hours after HIV exposure by panning with the ICH3 CD34 MoAb (ICH3/CD34+) and maintai

186 In the last round (round two) of panning with this library, none of the phage clones that

187 nsitions, instead offering smoothly animated panning, zooming, navigation, and track selection.