ライフサイエンスコーパス: parapertussis

1 saccharide plus an O-antigen-like repeat (B. parapertussis).

2 hooping cough in humans (B. pertussis and B. parapertussis).

3 ays for the detection of B. pertussis and B. parapertussis.

4 rger than that induced by B. pertussis or B. parapertussis.

5 human-adapted subspecies B. pertussis and B. parapertussis.

6 lla bronchiseptica and the human pathogen B. parapertussis.

7 nchiseptica (RB50), and other isolates of B. parapertussis.

8 i) phase Bordetella pertussis and Bordetella parapertussis.

9 hinzii, Bordetella pertussis, or Bordetella parapertussis.

10 . pertussis and B. bronchiseptica but not B. parapertussis.

11 trains of B. pertussis and two strains of B. parapertussis.

12 d 82% (95% CI, 69-90%) effectiveness against parapertussis.

13 parapertussis and cloned part of it from B. parapertussis.

14 is and also to a lesser extent by Bordetella parapertussis.

15 for both Bordetella pertussis and Bordetella parapertussis.

16 nd B. holmesii and 68% and 72% identified B. parapertussis.

17 rtussis, Bordetella holmesii, and Bordetella parapertussis.

18 of two palmitate acyl chains is unique to B. parapertussis.

19 PCR for Bordetella pertussis and Bordetella parapertussis.

20 sitive samples, 13.99% were identified as B. parapertussis.

21 lates, which were positive with IS1001 of B. parapertussis.

22 lated pathogens, Bordetella pertussis and B. parapertussis.

23 y of the acellular vaccine Adacel against B. parapertussis.

24 efficiently mediate opsonophagocytosis of B. parapertussis.

25 tion against B. pertussis but not against B. parapertussis.

26 tor is a potential protective antigen for B. parapertussis.

27 urface and complement-mediated killing of B. parapertussis.

28 50 (5,338,400 bp; 5,007 predicted genes), B. parapertussis 12822 (4,773,551 bp; 4,404 genes) and B. p

29 and 1,500 CFU/ml or 10 fg/mul of DNA for B. parapertussis A total of 1,103 fresh and residual frozen

30 ctiveness of pertussis vaccine in preventing parapertussis among Oregon children 2 months to 10 years

31 se are the first LPS mutants generated in B. parapertussis and B. bronchiseptica and the first deep r

32 Resistance is not efficiently acquired by B. parapertussis and B. bronchiseptica mutants lacking O an

33 to PT, we examined the ptx genes of both B. parapertussis and B. bronchiseptica to determine whether

34 We have found that B. parapertussis and B. bronchiseptica, unlike B. pertussis

35 Our analysis indicates that B. parapertussis and B. pertussis are independent derivativ

36 Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica are closely

37 pertussis was differentiated from Bordetella parapertussis and Bordetella bronchiseptica by hybridiza

38 The pathogenic bacteria Bordetella parapertussis and Bordetella bronchiseptica express a li

39 Bordetella parapertussis and Bordetella bronchiseptica resist killi

40 by the closely related pathogens Bordetella parapertussis and Bordetella bronchiseptica.

41 Bordetella parapertussis and Bordetella pertussis are closely relat

42 is also highly conserved in both Bordetella parapertussis and Bordetella pertussis.

43 in Bordetella bronchiseptica and Bordetella parapertussis and cloned part of it from B. parapertussi

44 of cytokines involved in the clearance of B. parapertussis and immunomodulation that delays effective

45 tigen is a critical protective antigen of B. parapertussis and its inclusion can substantially improv

46 ferentiate B. pertussis, B. holmesii, and B. parapertussis and provided protocols and training to 19

47 examined clinical features of patients with parapertussis and the effect of antibiotic use for treat

48 uate antibiotic effectiveness for preventing parapertussis and to determine risks and benefits of ant

49 s, 12 were positive (9 B. pertussis and 3 B. parapertussis) and 68 specimens were negative by all met

50 detella bronchiseptica, B. pertussis, and B. parapertussis) and its role in their biofilm development

51 ., including 4 of B. bronchiseptica, 5 of B. parapertussis, and 5 of B. pertussis, were studied.

52 nes are highly conserved in B. pertussis, B. parapertussis, and B. avium.

53 Bordetella pertussis, B. parapertussis, and B. bronchiseptica are closely related

54 es specificities of Bordetella pertussis, B. parapertussis, and B. bronchiseptica might be explained

55 ica cluster, which includes B. pertussis, B. parapertussis, and B. bronchiseptica.

56 d identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using multi

57 Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica are closely

58 ion of this gene in B. pertussis, Bordetella parapertussis, and Bordetella bronchiseptica by allelic

59 isaccharide on the LPS core is present in B. parapertussis, and further suggests that the wild-type w

60 n B. pertussis and B. holmesii; IS1001 of B. parapertussis; and the IS1001-like sequence of B. holmes

61 e toxins encoded by B. bronchiseptica and B. parapertussis are active.

62 Bordetella pertussis and Bordetella parapertussis are closely related endemic human pathogen

63 Although both B. pertussis and B. parapertussis are more closely related to B. bronchisept

64 species, Bordetella pertussis and Bordetella parapertussis are nonmotile human pathogens, while Borde

65 Here we show that B. pertussis and B. parapertussis are predominantly differentiated from B. b

66 tussis Tohama I, B. pertussis 18-323, and B. parapertussis ATCC 15311.

67 d, the batB gene of human-derived Bordetella parapertussis (B. parapertussis(hu)) contains a large in

68 ty-eight hours after infection, wild-type B. parapertussis bacteria but not the O antigen-deficient m

69 B. bronchiseptica but not B. pertussis or B. parapertussis bacterial numbers during the first 72 h.

70 e passive transfer of sera raised against B. parapertussis, but not B. parapertussis Deltawbm, reduce

71 Interestingly, B. parapertussis, but not B. pertussis, produces an O antig

72 B. parapertussis can also cause whooping cough, and B. bron

73 Bordetella parapertussis causes the prolonged coughing illness know

74 efficient protection against a subsequent B. parapertussis challenge.

75 d are well studied, the strain of Bordetella parapertussis chosen for sequencing is a recent human cl

76 Thus, the O antigen of B. parapertussis confers asymmetrical cross-immunity betwee

77 and found that both B. bronchiseptica and B. parapertussis contain at least certain of these genes, i

78 oth Bordetella bronchiseptica and Bordetella parapertussis contain regions homologous to the ptx gene

79 B. parapertussis contains a putative pagP homolog (encoding

80 with previously confirmed B. pertussis or B. parapertussis data and with data from 50 contrived B. pa

81 In B. bronchiseptica and B. parapertussis, delta wlb mutants also lacked O-antigen.

82 S), which contains the O antigen, but not B. parapertussis Deltawbm LPS drastically improved the effi

83 The B. parapertussis Deltawbm mutant was severely defective in

84 an isogenic mutant lacking the O antigen, B. parapertussis Deltawbm, induced antibodies that recogniz

85 raised against B. parapertussis, but not B. parapertussis Deltawbm, reduced B. parapertussis loads i

86 B. pertussis and B. parapertussis Deltawlb mutants were also defective compa

87 mercial laboratories for B. pertussis and B. parapertussis detection.

88 Since B. parapertussis did not cause severe disease in IL-1R(-/-)

89 In contrast, LpxA from B. parapertussis did not display relaxed specificity but wa

90 gly, serum antibody-mediated clearance of B. parapertussis did not require Fc receptors that are requ

91 ssay, which detects both B. pertussis and B. parapertussis directly from nasopharyngeal swab specimen

92 in TLR4-deficient mice, B. pertussis and B. parapertussis do not.

93 that also causes whooping cough, Bordetella parapertussis, does not.

94 of infection, immunization with wild-type B. parapertussis elicited a strong antibody response to the

95 pertussis, B. bronchiseptica, or Bordetella parapertussis eliminated the clumped-growth phenotype an

96 a has a wide host range, B. pertussis and B. parapertussis evolved separately from a B. bronchiseptic

97 Bordetella bronchiseptica and Bordetella parapertussis express a surface polysaccharide, attached

98 ted the simple and effective isolation of B. parapertussis from ovine nasal swabs and, in successfull

99 f leukocytes in lungs and in clearance of B. parapertussis from the lungs.

100 s an improved selective medium to isolate B. parapertussis from the nasal cavities of conventionally

101 detella pertussis, B. bronchiseptica, and B. parapertussis genome assemblies permitted the identifica

102 The increasing incidence of B. parapertussis has been attributed to the lack of cross p

103 of Bordetella bronchiseptica and Bordetella parapertussis have DNA homologous to vag-8.

104 ertussis have been well studied, those of B. parapertussis have not.

105 septica, Bordetella pertussis and Bordetella parapertussis have the recycling/salvage pathway genes p

106 ous bordetellae, Bordetella pertussis and B. parapertussis, have emerged in historical times as co-do

107 according to pathogen host range and that B. parapertussis(hu) most likely acquired its fhaS allele f

108 Notably, the genes present in B. parapertussis(hu) strains were pseudogenes, and the gene

109 es both human-infective (B. pertussis and B. parapertussis(hu)) and non-human-infective (B. bronchise

110 f human-derived Bordetella parapertussis (B. parapertussis(hu)) contains a large in-frame deletion re

111 ng inoculation with B. pertussis, but not B. parapertussis, IL-1R(-/-) mice showed elevated bacterial

112 eltawbm) mutants of B. bronchiseptica and B. parapertussis in a variety of assays relevant to natural

113 Attempts to assess the prevalence of B. parapertussis in conventionally reared sheep by nasal sw

114 ment in the detection of B. pertussis and B. parapertussis in nasopharyngeal specimens.

115 ting and differentiating B. pertussis and B. parapertussis in nasopharyngeal swab specimens.

116 ntiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was developed.

117 ant investigation of the relative role of B. parapertussis in the resurgence of whooping cough.

118 ction of Bordetella pertussis and Bordetella parapertussis In this study, we evaluated the performanc

119 apting to infect humans, B. pertussis and B. parapertussis independently modified their LPS to reduce

120 Together, these data indicate that B. parapertussis induces the production of IL-10, which fac

121 Bordetella parapertussis infection causes pertussis-like illness th

122 chiseptica, while no role for TLR4 during B. parapertussis infection has been described.

123 pertussis vaccines have little effect on B. parapertussis infection or disease suggest that the prot

124 Immunity induced by B. parapertussis infection protected against subsequent inf

125 In addition, nine cases of B. parapertussis infection were also confirmed by using the

126 It was concluded that B. parapertussis infections are more common than previously

127 After finding that several children with B. parapertussis infections exhibited modest antibody titer

128 statewide pertussis outbreak, 443 Bordetella parapertussis infections were reported among Wisconsin r

129 ction and distinction of B. pertussis and B. parapertussis infections within 2 h.

130 is infections but did not protect against B. parapertussis infections.

131 The O antigen of B. parapertussis inhibited binding of antibodies to the bac

132 Here, we evaluated the outcome of B. parapertussis innate interaction with human macrophages,

133 Bordetella parapertussis is a human pathogen that causes whooping c

134 It was previously shown that B. parapertussis is able to avoid bacterial killing by poly

135 ent study explores the mechanism by which B. parapertussis is cleared from the lower respiratory trac

136 trometry analysis revealed that wild-type B. parapertussis lipid A consists of a heterogeneous mixtur

137 The addition of 10 microg of purified B. parapertussis lipopolysaccharide (LPS), which contains t

138 ut not B. parapertussis Deltawbm, reduced B. parapertussis loads in the lower respiratory tracts of m

139 old more stimulatory than B. pertussis or B. parapertussis LPS, respectively.

140 etella bronchiseptica (lpxA(Br)), Bordetella parapertussis (lpxA(Pa)), and Bordetella pertussis (lpxA

141 tes accumulated in the lungs, and cleared B. parapertussis more rapidly.

142 ation of Bordetella pertussis and Bordetella parapertussis nucleic acids in nasopharyngeal swab (NPS)

143 These results highlight the need for B. parapertussis opsonic antibodies to induce bacterial cle

144 ere pseudogenes, and the genes present in B. parapertussis(ov) strains were expressed at significantl

145 on-human-infective (B. bronchiseptica and B. parapertussis(ov)) strains.

146 ontains a putative pagP homolog (encoding B. parapertussis PagP [PagPBPa]), but its role in the biosy

147 nd receipt of azithromycin prophylaxis among parapertussis patient household members (HHMs) were also

148 Among 218 patients with parapertussis, pertussis-like symptoms were frequently r

149 pertussis positive and 0.2% (n = 2) were B. parapertussis positive.

150 We also observed a notable increase in B. parapertussis positivity on nonpanel PCR tests in the fi

151 ons in the locus in B. bronchiseptica and B. parapertussis prevent O-antigen biosynthesis and provide

152 Bordetella parapertussis, previously thought to be an obligate huma

153 The toxin encoded by the B. parapertussis ptx genes appeared more labile in culture

154 The B. bronchiseptica and B. parapertussis recipients were now able to biosynthesize

155 erase chain reaction results positive for B. parapertussis reported during October 2011-May 2012 were

156 nces IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using the

157 lation of CD4(+) T cells in the lungs and B. parapertussis-responsive IFN-gamma-producing cells in th

158 wlb locus of Bordetella bronchiseptica or B. parapertussis restored partial sensitivity to Ba1.

159 sis does not express the O antigen, while B. parapertussis retains it as a dominant surface antigen.

160 strains of B. pertussis and one strain of B. parapertussis revealed extensive divergence of gene orde

161 ssis data and with data from 50 contrived B. parapertussis samples, the proportions of positive and n

162 ssis strain 18323 and an ovine isolate of B. parapertussis show significant transcription of the gene

163 l bordetellae, including B. pertussis and B. parapertussis, something the current vaccines do not pro

164 In vitro B. parapertussis-stimulated macrophages produced IL-10, whi

165 assay detected 16/18 unique B. pertussis/B. parapertussis strains.

166 efficacy of B. pertussis vaccines against B. parapertussis suggest a lack of cross-protective immunit

167 control and clearance of B. pertussis or B. parapertussis, suggesting that IgA is not crucial to imm

168 hat in the absence of opsonic antibodies, B. parapertussis survives inside macrophages by preventing

169 at might be misclassified as pertussis if B. parapertussis testing is not performed.

170 ture, PCR (both nonpanel B. pertussis and B. parapertussis tests and those included as part of a resp

171 ge genetic locus in B. bronchiseptica and B. parapertussis that is required for O-antigen biosynthesi

172 r the mechanism of protective immunity to B. parapertussis that is similar but distinct from that of

173 % for B. pertussis and 100% and 99.7% for B. parapertussis The Aries Bordetella Assay provides accura

174 ) for B. pertussis and 213 CFU.ml(-1) for B. parapertussis The assay detected 16/18 unique B. pertuss

175 There were no samples positive for B. parapertussis The PPA and NPA of the Aries BA were 61.1%

176 ice with Bordetella pertussis and Bordetella parapertussis, the causative agents of whooping cough.

177 he bacterial surface and was required for B. parapertussis to colonize mice convalescent from B. pert

178 t O antigen contributes to the ability of B. parapertussis to colonize the respiratory tract during t

179 hese data indicate that O antigen enables B. parapertussis to efficiently colonize the lower respirat

180 the absence of opsonins, O antigen allows B. parapertussis to inhibit phagolysosomal fusion and to re

181 The O antigen targets B. parapertussis to lipid rafts that are retained in the me

182 In addition, O antigen was required for B. parapertussis to systemically spread in complement-suffi

183 ction of Bordetella pertussis and Bordetella parapertussis using molecular methods is sensitive and s

184 In other assays, B. parapertussis was distinct from all other species (in pi

185 B. parapertussis was more similar to B. bronchiseptica than

186 stingly, an O antigen-deficient strain of B. parapertussis was not defective in colonizing mice lacki

187 B. parapertussis was not detected in any specimens.

188 abs from conventionally reared sheep, and B. parapertussis was recovered from 31.5% of the samples.

189 The overall PPA and NPA for B. parapertussis were 96.7% and 100%, respectively.

190 In the case of B. parapertussis, which normally does not synthesize an app

191 e persistence of Bordetella pertussis and B. parapertussis within vaccinated populations and the reem

192 om a range of bacteria, is altered in the B. parapertussis WlbH protein.

193 etella bronchiseptica (wlbbr) and Bordetella parapertussis (wlbpa) were identified and cloned.