ライフサイエンスコーパス: passage

1 ) of the corresponding virulent strains (low passage).

2 passaging that are significant in the sixth passage.

3 :12% for A(H3N2) viruses after a single cell passage.

4 occludin expression and facilitated allergen passage.

5 , sequencing the microbial community at each passage.

6 ash of intuition about the reality of time's passage.

7 ubsequently reproduced after serial in vitro passage.

8 s analysis in comparison to cells of earlier passage.

9 eage-biased stem cell state through multiple passages.

10 th a pharmacotyping profile similar to early passages.

11 educed susceptibility to remdesivir after 35 passages.

12 22L, RML, and ME7) and cultured them for six passages.

13 nd remained in such condition after multiple passages.

14 rions replicated only very weakly after five passages.

15 oviruses are attenuated and pseudo-revert on passaging.

16 genetically stable over 10 rounds of serial passaging.

17 ) was introduced to chickens and isolated as passage 1 (Cj-P1).

18 In contrast, a low-passage-number (passage 10 [P10]) MHV-ExoN-AA showed increased replicati

19 e 5 and that these mutations persisted until passage 10.

20 (G1P[8]), which when grown in MA104 cells to passage 11 or 12 (P11/P12) had no nucleotide or amino ac

21 ltured H1 human embryonic stem cells (WA-01, passage 27-40) and induced pluripotent stem cells (GM233

22 Each virus was serially passaged 32 times (which accounts for more than 60 viral

23 hemagglutinin (HA) glycoprotein as early as passage 5 and that these mutations persisted until passa

24 Here, we serially passage a 34-species defined bacterial community through

25 t work with antibiotics to selectively allow passage across bacterial membranes.

26 Passage across is mediated by host karyopherins (KPNAs),

27 o, high tissue uptake in vivo, and efficient passage across the blood-brain barrier.

28 n, and plant phosphorus (P) uptake after its passage across the Florida Coastal Everglades in Septemb

29 rmation at the end of accommodation corridor passage after EF-Tu release can be reengaged by EF-Tu.GT

30 unction facilitates transepithelial allergen passage, allergic sensitization, and allergen-induced MC

31 ejection of milk from alveolar units and its passage along the mammary ductal network.

32 We can unmistakably sense its passage and adjust our behavior accordingly.

33 c conditions and by the opening of the Drake Passage and associated intensification of the Antarctic

34 reducing cell death during gastrointestinal passage and controlling the release of these therapeutic

35 We include details of how to passage and cryopreserve GBOs for continued use, biobank

36 cessful tracheal intubation, time to glottis passage and first end-tidal CO2 measurement, degree of g

37 ponses to climate change, and that improving passage and fluvial connectivity will be important clima

38 Sequencing of genomes of passaged and cloned viruses revealed mutations near the

39 e binary cocultures obtained could be stably passaged and studied.

40 osed to neuroendocrine differentiation along passaging and are relatively more castration-resistant.

41 neural stem cells (NSC) during their serial passaging and differentiation.

42 growth or stone disappearance from presumed passage) and determine stone composition based on mutual

43 een wave variability and atmospheric frontal passages, and (2) the influence on the SST variations du

44 s commonly detected after two rounds of cell passage; and (iii) the PRRA insertion, which is unique t

45 oretical and practical aspects of the double-passage approach, prove its validity quantitatively, and

46 vitro culture for at least four cell culture passages (approximatively 10 cell doublings).

47 ever, timing and reason for this cytoplasmic passage are poorly understood.

48 cate that the LMC and SMC are on their first passage around the Galaxy(5), that the Magellanic Stream

49 x vivo cell cultures examined before the 3rd passage as well as the primary and virus-transformed cel

50 atient liver specimen was undetectable after passage as xenograft tumors in mice.

51 Passaging assays for resistance did not produce any resi

52 Then, the cysts were en bloc-passaged, attached to culture surface, and grew, forming

53 caca fascicularis) macaques with a minimally passaged Brazilian ZIKV isolate and used multicolor flow

54 insic to CMG; however, Mcm10 promotes strand passage by enhancing the affinity of CMG to DNA.

55 ntains chromatin integrity during polymerase passage, by facilitating removal of the H2A-H2B dimer, s

56 otype of both patient tumors and derived low-passage cell lines were characterized by immunohistochem

57 Subcloning these late passage cells to clonal density, to mimic lung injury in

58 uated vaccine candidates or low-cell culture passage clinical isolates from humans or animals.IMPORTA

59 The competitiveness of low-passage commensal streptococcal clinical isolates is pos

60 es, we observed a strong signal over all six passages, comparable with that for 22L-infected cells.

61 increases in the length of time to read the passage compared to baseline (161 vs 172 wpm, respective

62 nce of any new stone, stone growth, or stone passage (comparing baseline and follow-up scans).

63 supercoil introduction, beyond which strand passage competes with ATP-dependent supercoil relaxation

64 alyze patterns of substitutions in different passage conditions for an unprecedented amount of H3N2 h

65 Serial passages continuous up to sixth passages in the control

66 nosine abrogates colony growth in these late passage cultures (>200 PD) but not in early passage cult

67 passage cultures (>200 PD) but not in early passage cultures (<200 PD).

68 9 expression is lost with volar keratinocyte passaging, despite stable hypomethylation of its promote

69 Abdominal X-ray showed no signs of passage disorder.

70 resistance profile of remdesivir by serially passaging Ebola virus under remdesivir selection; we gen

71 LIV effects were assessed at both early passage (EP) and late passage (LP).

72 ganoids can be established within 2-3 weeks, passaged every 7-10 d, and cultured for over a year.

73 We used a successive passaging experiment to address this question by selecti

74 Although TMUV can be attenuated by in vitro passaging, experimental evidence supporting the role of

75 firm the antiviral drug target, serial viral passage experiments were performed to select for resista

76 as developed, and the tumor was successfully passaged for at least three rounds in immunodeficient mi

77 esected tumors and biopsies and cultured and passaged for prolonged periods.

78 negative Hyalomma anatolicum tick pools were passaged for virus isolation, and two virus isolates wer

79 broblasts were maintained in culture without passaging for 4 or 8 days (treatment studies).

80 s show that even low levels of random strand passage, for example by topoisomerase II, would result i

81 t means of infection, especially in terms of passage from an infected to an uninfected partner.

82 A second PCT passage further reduced the volatiles (36%) and is thus

83 on of colonic goblet cell-associated antigen passages (GAPs), which translocate enteric bacteria acro

84 erization of the cultured CEnC across serial passages has not been performed.

85 y and persistently degraded by the minimally passaged HCMV strain Merlin but not the extensively pass

86 ccurred in late-passage hiPSCs than in early-passage hiPSCs after differentiation.

87 r differentiation ability of early- and late-passage hiPSCs into cardiomyocyte-like, hepatic-like, an

88 trends, in which more SNPs occurred in late-passage hiPSCs than in early-passage hiPSCs after differ

89 tions within G1 that occurred earlier in the passage history of the Can strain also contribute to agg

90 The human nasal passages host a distinct community of microbes.

91 on in a harsh challenge setup using an early-passage human patient isolate.

92 stone in 35%, stone growth in 24%, and stone passage in 27%.

93 The kinetics and intensity of Ara h 6 passage in bloodstream depend on both individual and foo

94 ency of tdTom(+) cells increased with serial passage in cultured murine embryo fibroblasts from p16 (

95 nce and facilitated transepithelial allergen passage in cultured primary nasal epithelial cells.

96 transmission to progeny worms during serial passage in lab colonies.

97 ese mice remained fully virulent in a second passage in mice.

98 We used serial passage in ribavirin, beginning with a variety of ribavi

99 Further, passage in the presence of NHC generates only low-level

100 Atp2b1(-/-)Tcf7l1(-/-) double-knockout ESCs passaged in defined media alone (no LIF or inhibitors) a

101 e nucleotide polymorphisms (SNPs) from virus passaged in dose escalation studies in a nonhuman primat

102 When passaged in host environments that restrict prion replic

103 no LIF or inhibitors) and in wild-type cells passaged in media containing only calcitonin and a GSK3

104 smids were propagated in MDCK cells and then passaged in mice up to four times.

105 f PDK isoforms 1 and 3 that persisted for >5 passages in culture.

106 Adaptation of IDV by serial passages in mice was not sufficient to induce disease or

107 Serial passages continuous up to sixth passages in the control group.

108 Upon adaptation and passages in Vero cells, the strain underwent five amino

109 he I38T substitution remained stable after 4 passages in vitro.

110 virus vaccines have been developed by serial passaging in cell culture and found to be safe in infant

111 ral of these mutations were stable following passaging in the absence of ML336 in Vero 76 cells.

112 pike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resu

113 Expansion by continuous passaging induced replicative cell senescence.

114 preventing DNA slippage, whilst allowing its passage into the capsid, and how these processes are con

115 h selectivity for potassium over sodium; ion passage is blocked by specific chemical modification at

116 colonization, we examined cagY in 534 mouse-passaged isolates that lost T4SS function, defined as a

117 For example, the highly passaged Ixodes scapularis cell line ISE18 and Ixodes ri

118 Gain of function of 46 kDa Pax6 in late-passaged LNC resulted in nuclear Pax6 staining and promo

119 assessed at both early passage (EP) and late passage (LP).

120 partially uncouples ATP turnover from strand passage, manifesting in futile cycling.

121 both $K$ and $\Delta Lk$ by a duplex-strand-passage mechanism and have been shown to simplify the to

122 In addition, a first-passage method to evaluate the dynamics of transcription

123 On first passage, mice challenged with LL132 elk inoculum had pro

124 er-to-passage ratio (SPR), the percentage of passage migrants that stop in an area, and use 8 years o

125 om pupae with high DWV levels but not in the passaged mites.

126 Mass spectroscopy of late passage MSC indicated a synergistic decrease of actin an

127 s aging through a `semi-natural' process, by passaging multiple times at the Neural Precursor Cell st

128 y resolved stratigraphic record of hurricane passage near Thatchpoint Blue Hole (TPBH) on Abaco Islan

129 treamlines is often aperiodic: Repeated slow passages near stagnation points and/or solid surfaces se

130 At low passage NPCs (P1 to P3), we observed cell cycle arrest,

131 Optimal conditions including cell density, passage number, and culture time were examined to fabric

132 In contrast, a low-passage-number (passage 10 [P10]) MHV-ExoN-AA showed inc

133 Low-passage-number NF54 parasites exposed to 8.2 mM lactic a

134 Despite the passage of a decade since the tragic loss of an organ re

135 te model of gastric motility that traces the passage of a food bolus through the stomach over a 30 mi

136 hanges in EF-Tu coordinate the rate-limiting passage of aa-tRNA through the accommodation corridor en

137 Transepithelial passage of allergens was evaluated on primary human nasa

138 S has been associated with transgenerational passage of altered behavioral phenotypes, whereas the tr

139 lipid nanocarriers for facilitating the BBB passage of an ARV darunavir.

140 ene-inactivating frameshift mechanism during passage of an attenuated VACV.

141 e-strand break (DSB) in one DNA molecule and passage of another intact DNA duplex through the break.

142 can emerge as a dominant prion strain during passage of AS between different species.

143 oreal summer, presumably associated with the passage of atmospheric African Easterly Waves.

144 uisition of adaptive mutations during serial passage of attenuated vaccinia virus (VACV).

145 x pore-but not the 12-helix pore-enables the passage of biotinylated Alexa Fluor 488.

146 assage of mucus with BM (UC 67.7%/CD 36.9%), passage of blood from the anus (UC 59.7%/CD 32.1%), and

147 greatest differences between UC and CD were passage of blood with BM (UC 86.6%/CD 45.3%), urgent BM

148 mptoms by hospital characteristics after the passage of California's safe patient handling legislatio

149 mental model of how NPCs can facilitate fast passage of cargoes across an order of magnitude in cargo

150 in claudin-5 (cldn5) in male mice, promoting passage of circulating proinflammatory cytokines and dep

151 tself as a plausible channel that allows the passage of dNTPs into assembled capsids.

152 endothelial permeability, as measured by the passage of fluorescein isothiocyanate (FITC)-dextran thr

153 es acts as a genetic link or conduit for the passage of genes (alleles) between two others that rarel

154 Geospiza fortis acted as a conduit for the passage of genes between two others that have never been

155 Tracing the possible passage of incoming UTP or GTP through the RdRp-specific

156 mbrane form of the 12-helix pore enables the passage of ions across the membrane with high selectivit

157 annels are not just passive conduits for the passage of ions and metabolites but have been shown to g

158 e that these changes allow the transcallosal passage of ipsilateral eye input to lateral striate cort

159 rted membrane, it acts as a net, slowing the passage of large ions while letting smaller ions through

160 ate of the Sec61 channel, priming it for the passage of low-hydrophobicity signal sequences into the

161 entral lipid core facilitates the controlled passage of membrane proteins into the bilayer, ensuring

162 shell serves as a semi-permeable barrier for passage of metabolites in and out of the carboxysome to

163 6%/CD 45.3%), urgent BM (UC 82.5%/CD 63.9%), passage of mucus with BM (UC 67.7%/CD 36.9%), passage of

164 Cell membrane transporters facilitate the passage of nucleobases and nucleosides for nucleotide sy

165 us particles such as bacteria but allows the passage of nutrients and drug molecules towards the inte

166 on the pathophysiological relevance of rapid passage of nutrients to the small bowel, on the role of

167 The latter presumably drives efficient passage of OMPs.

168 The new technique begins with the passage of one of two straight needles existing at each

169 This time period coincides with passage of our SS through such interstellar clouds, whic

170 Because passage of Pol II through +1 nucleosomes genome-wide wou

171 assemble a multinucleate cytoplasm, allowing passage of proteins of at least 62 kDa throughout papill

172 g programs in California hospitals after the passage of safe patient handling legislation.

173 eases in earthquake rate coincident with the passage of seismic energy.

174 electively or are large enough to enable the passage of small-molecule fluorophores remains an outsta

175 s in the cancer care delivery system such as passage of the Affordable Care Act, cancer disparities p

176 verage and care that have occurred after the passage of the Affordable Care Act, the rapidly changing

177 , along the tube to the capsid that triggers passage of the DNA down the tube and into the host bacte

178 ovide a clear signature of the non-adiabatic passage of the excited state wavepacket through a conica

179 hin the variable domain allows for continued passage of the partially processed precursors through th

180 transiently by DNA-binding factors following passage of the replication fork, which may provide a mec

181 of the sample material before and after the passage of the shock derived from conservation laws) of

182 ons critical to episodic memory code for the passage of time at a range of timescales.

183 e propose two analyses, namely, decoding the passage of time from neural data and computing the cumul

184 We found that the passage of time was associated with brain regions associ

185 mory for temporal rules and attention to the passage of time.

186 intimately related to our experience of the passage of time.

187 The Sec channel enables passage of unfolded proteins through the bacterial plasm

188 t of plant rhabdoviruses likely involves the passage of viral nucleocapsids through MP-gated plasmode

189 n membranes and demonstrate pathways for the passage of water molecules.

190 evelop along the membrane surface due to the passage of water through the membrane, a process known a

191 rimental evolution protocol involving serial passages of an attenuated vaccinia virus, rapid acquisit

192 pears to be similar in virulence to previous passages of NV strain 8fIIa.

193 x different genes occurred in three parallel passages of the attenuated virus.

194 the end of primary culture, six serial cell passages of the control group, before and after of the d

195 gthened by routing the ions through multiple passages of the same path.

196 These changes were maintained in subsequent passages of tumor xenografts in vivo and in cell lines e

197 High multiplicity of infection passaging of both NiV clinical isolates and recombinant

198 ransfer (AFCT) reactions, to support routine passaging of intestinal organoids.

199 of additional genes can occur upon extensive passaging of orthopoxviruses in cell culture leading to

200 otodegradation is used to achieve repetitive passaging of stem cell colonies without loss in morpholo

201 H-HP and LV39-HP-by continuous culture (high passage) of the corresponding virulent strains (low pass

202 virus in neutralized samples was assessed by passage on cells and evaluation for viral cytopathic eff

203 However, after 8 additional days of passage on honey bee pupae with low viral loads, the DWV

204 rties of DENVs in human plasma and after one passage on laboratory cell lines.

205 It allows passage on nutrients/pharmaceuticals from the gut lumen

206 the wave data in response to the cold front passages over Louisiana continental shelf.

207 was significantly higher than in the primary passage (P < 0.05).

208 ast cells (all, P < .0001), and paracellular passage (P = .02) were significantly higher in FD-starte

209 twice-daily LIV regimen for up to sixty cell passages (P60) under in vitro cell expansion conditions.

210 a inhibitor, PT2385, both in vitro, with low-passage patient-derived cell lines, and in vivo, using o

211 ministered intravenously to mice bearing low-passage, patient-derived pancreatic cancer xenografts (P

212 ehavior of the gas-solid mixture in the flow passage pipeline were studied by numerical simulation.

213 phenotypic characteristics are maintained in passaged primary NHBE cells.

214 Furthermore, extensions to adiabatic passage problems in more-level systems are straightforwa

215 Serially passaged progenitors demonstrated shorter telomeres (P <

216 pal progenitor cells in vitro using a serial passaging protocol that mimics the end-replication probl

217 ur levels and three interlevel ion escalator passages, providing a total path length of 43.2 m.

218 l vasodilators may improve spontaneous stone passage rates and reduce the pain caused by ureteral ste

219 Here, we introduce the stopover-to-passage ratio (SPR), the percentage of passage migrants

220 On second passage, relative incubation periods, western blot profi

221 These ICs can be passaged, replicate rapidly and remain confluent beyond

222 ne permeability and ATP release, with serial passaging resulting in a mutation in mlaC, a phospholipi

223 During 10 passage rounds, the attenuated VACV was replaced by the

224 After only 10 passage rounds, the starting attenuated vaccinia virus w

225 e plants across 5 plant genotypes for 4 45-d passages, sequencing the microbial community at each pas

226 during in vitro expansion of MSCs for sixty passages significantly alters MSC proliferation, differe

227 human iPSC-derived motor neurons to serially passaged sporadic ALS post-mortem tissue (spALS) extract

228 d HCMV strain Merlin but not the extensively passaged strain AD169.

229 sociated with NH(4) (+) deprotonation during passage, substantially transported K(+) in addition to N

230 on the widespread stimulated Raman adiabatic passage technique, which achieves these objectives for q

231 d in fewer dominating mutations during viral passaging than MDCK cells.IMPORTANCE Robust in vitro cul

232 azon Kindle application, we demonstrate that passages that people highlighted-collectively, over a qu

233 ely to contain generic-you compared to yoked passages that they did not highlight.

234 ies and the intensity is increased by serial passaging that are significant in the sixth passage.

235 Furthermore, we serially passaged the KDKE(4A)-SYA mutant in pigs three times and

236 MVA was derived by extensively passaging the chorioallantois vaccinia virus Ankara (CVA

237 optimized for the macaque model by serially passaging the engineered virus in vitro or in vivo Recen

238 Previously, serially passaging the H7N9 A/Anhui/1/2013 virus in the presence

239 On long-term passage, the mutant cells stably produced proteinase K (

240 Remarkably, after this cytoplasmic passage, the nuclear stability of telomerase RNA no long

241 between Ixodes spp. cell lines at different passages, their karyotypes differed from 2n = 28 chromos

242 ale germline deletion, but is lost following passage through a wild type germ line.

243 H2Bub) facilitate RNA polymerase II (Pol II) passage through chromatin, yet it is not clear how they

244 Plvap limits the rate of blood-borne protein passage through fenestrated endothelia.

245 e of first northward migration and the later passage through Iceland during northward migration of tr

246 interstellar particle density during Earth's passage through local interstellar clouds, which could b

247 he levels of centrosomal microtubules during passage through mitosis.

248 es measuring radio-frequency emissions after passage through normal matter) should be able to detect

249 -specific subunit domains to efficient Pol I passage through nucleosomes in the context of transcript

250 ubunits Rpa34.5/Rpa49 and Rpa12.2 facilitate passage through nucleosomes, suggesting possible coopera

251 omes to overcome numerous impediments during passage through open (eu) and condensed (hetero) chromat

252 tic radiation at ~ 10(4) to ~ 10(9) Hz after passage through planetary environments.

253 modulate the motive force that enables their passage through restrictive pores in complex three-dimen

254 regulation to explain how cell size controls passage through Start.

255 d stripped of membranes by bile salts during passage through the bile ducts to the gut(4).

256 %) in the intact glucosinolate contents upon passage through the gastrointestinal (GI) tract.

257 ted from the eye and become myelinated after passage through the optic nerve lamina region (ONLR), a

258 This effect increases following passage through two successive generations of Kdm6a male

259 eggs of killifish have been found to survive passage through waterbird guts.

260 and phenotypic characteristics of CWD prions passaged through deer and transgenic mice expressing dif

261 Thus, highly passaged tick cell lines can be used for research purpos

262 We used first-passage time analysis to determine the scale at which So

263 xact calculation of the diffusive mean first-passage time on a spatial network is used to characteriz

264 first and second moments of a general first-passage time problem in the limit of large, but finite,

265 s the free energy profile and the mean first passage time.

266 Singularities of passage times near the obstacles, dictated by the bounda

267 e the analytical distribution of these first-passage times.

268 n that conjoined future a link to Art, and a passage to a renewed vision of the sacred in science.

269 n that conjoined future a link to Art, and a passage to a renewed vision of the sacred in science.

270 creation provides in that conjoined future a passage to art and to perceiving, as we argue we must, t

271 d bacterium, ultimately forming a continuous passage to the host, for delivery of virulence effectors

272 , habitat use has never been integrated with passage to understand the factors that determine where a

273 during normal evolution and extensive virus passaging to make vaccine strains.

274 Atlantic seaboard, culminating in the great passage tomb complexes, is particularly impressive(2).

275 lution for a prehistoric population) between passage tomb samples and the larger dataset, which toget

276 n the most elaborate recess of the Newgrange passage tomb.

277 dividual within two other major complexes of passage tombs 150 km to the west of Newgrange, as well a

278 contractile ductal network, milk begins its passage toward the dependent neonate, seconds after the

279 thelial cells were cultured through multiple passages under normoglycaemic (5.5 mM) or hyperglycaemic

280 Primary cells passaged up to four times maintained airway epithelial c

281 Here, we describe serially passaging VACV in nonpermissive cells expressing A56/K2

282 We passaged VEEV TC-83 in these two cell lines over seven c

283 adapts to different hosts, we experimentally passaged viral populations between two alternate hosts,

284 We have also identified two groups of goat-passaged viruses which have acquired an extra 6 bases in

285 notypic changes were observed for MIA PaCa-2-passaged viruses, both SUIT-2-passaged VSV-p53wt and VSV

286 ns of virus-carried transgenes in any of the passaged viruses, demonstrating long-term genomic stabil

287 4E and E238K, were identified in both SUIT-2-passaged viruses.

288 n the virus-carried transgenes in any of the passaged viruses.

289 nous dsRNA inhibits KRT9 expression in early passage volar keratinocytes or in vivo footpads of wild-

290 for MIA PaCa-2-passaged viruses, both SUIT-2-passaged VSV-p53wt and VSV-p53-CC showed improved replic

291 The UPE of the NSC in the sixth subculturing passage was significantly higher than in the primary pas

292 EPR effect offers a possible route for drug passage, we further explore alternative uses of NPs to c

293 tein, although variations in the kinetics of passage were observed between individuals and matrices.

294 ytes propagated 22L prions well over all six passages, whereas ME7 prions did not replicate, and RML

295 nces were faithfully maintained after serial passaging, which implies that alpha-synuclein propagates

296 of this interaction was obtained by serially passaging wild-type VACV in cells that are nonpermissive

297 e transient wavepacket coherence during this passage with an X-ray free-electron laser pulse.

298 lium cultures can be cryopreserved, serially passaged without loss of identity, and transitioned towa

299 Only 0.2% of ingested eggs survived gut passage, yet, given the abundance, diet, and movements o

300 tigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and