ライフサイエンスコーパス: peptide N-glycosidase

1 ide and the peptide moieties are released by peptide-N-glycosidase.

2 Controlled deglycosylation using peptide : N-glycosidase F and endo-beta-N-acetylglucosam

3 saccharide mapping, bands were digested with peptide N-glycosidase F (PNGase F) in order to release t

4 AKC patients were processed and treated with peptide N-glycosidase F (PNGase F) to deglycosylate N-gl

5 ting membrane preparations were treated with peptide N-glycosidase F (PNGase-F).

6 Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues f

7 Moreover, treatment with peptide N-glycosidase F abrogated F240 neutralization, i

8 Peptide N-glycosidase F and endoglycosidase H deglycosyl

9 igestion with Flavobacterium meningosepticum peptide N-glycosidase F and trypsin, with matrix-assiste

10 PSA on NRP-2 is resistant to digestion with peptide N-glycosidase F but is sensitive to release unde

11 -1,4-galactosyltransferase or susceptible to peptide N-glycosidase F corresponded directly to their r

12 ther endoglycosidase F or H but sensitive to peptide N-glycosidase F digestion.

13 Pretreatment of the lactoferrin with peptide N-glycosidase F or addition of heparin or chondr

14 removal of the oligosaccharide chains using peptide N-glycosidase F or removal of the glucoses by ER

15 d ABCG2 are glycosylated, and treatment with peptide N-glycosidase F reduces the apparent molecular m

16 Treatment of kidney membrane proteins with peptide N-glycosidase F showed that GLUT9 and GLUT9Delta

17 Etanercept was first treated with peptide N-glycosidase F to release the N-glycans.

18 N-Glycosylation was responsible because peptide N-glycosidase F treatment of isolated 170-kDa EG

19 Peptide N-glycosidase F, but not endoglycosidase H, dige

20 zymatically released from glycoproteins with peptide N-glycosidase F, followed by purification with g

21 of 7.5 h and was sensitive to treatment with peptide N-glycosidase F, sialidase alone, or sialidase a

22 ATPase was examined by using tunicamycin and peptide N-glycosidase F, two agents used to prevent and

23 of the cells with trypsin, endoglycosidase F/peptide N-glycosidase F, Vibrio cholerae neuraminidase,

24 Using mass spectrometry on purified and peptide N-glycosidase F-deglycosylated CD36 and also by

25 f approximately 160 kDa after treatment with peptide N-glycosidase F.

26 ecifically inhibited by deglycosylation with peptide N-glycosidase F.

27 Deglycosylation of the receptor with peptide N:glycosidase F results in a decrease in molecul

28 formerly N-linked glycosylated peptides via peptide- N-glycosidase F (PNGase F).

29 not recognized by the classical glycosidases peptide-N-glycosidase F (PNGase F) and endoglycosidase H

30 specificities of endoglycosidases such as a peptide-N-glycosidase F (PNGase F) and of endo-N-acetlyg

31 Changes in electrophoretic mobility after peptide-N-glycosidase F (PNGase F) digestion suggest tha

32 A novel online enzyme reactor incorporating peptide-N-glycosidase F (PNGase F) on a monolithic polym

33 PO antibodies, dual digestion by trypsin and peptide-N-glycosidase F (PNGase F), and analysis by LC-M

34 transfected with GPVI, deglycosylation with peptide-N-glycosidase F (PNGase F; specific for complex

35 A deglycosylation step using Peptide-N-Glycosidase F (PNGaseF) has been introduced in

36 inked monosaccharides based on resistance to peptide-N-glycosidase F and analysis of saccharides rele

37 Deglycosylation using peptide-N-glycosidase F and site-directed mutagenesis es

38 Peptide-N-glycosidase F digestion of solubilized hKOR or

39 N-deglycosylation by peptide-N-glycosidase F digestion resolved these isoform

40 on analyses of Man(6)GlcNAc(2) released with peptide-N-glycosidase F from invertase secreted by Delta

41 After a simple and fast incubation using peptide-N-glycosidase F on target, sequential mass shift

42 Treatment with peptide-N-glycosidase F reduced the size of the mammalia

43 Treatment of AGMK and cr5 cell extracts with peptide-N-glycosidase F resulted in the collapse of the

44 Treatment of heart lysates with peptide-N-glycosidase F revealed that while giant obscur

45 were glycoproteins and molecular mass after peptide-N-glycosidase F treatment was 38 and 45 kDa, res

46 Using pulse-chase radiolabeling, peptide-N-glycosidase F treatment, lectin pulldowns, and

47 Both trifluoromethanesulfonic acid and peptide-N-glycosidase F treatments yielded a 50-kDa band

48 eleased from the antibody via digestion with peptide-N-glycosidase F, then derivatized with a charged

49 hy following release from the polypeptide by peptide-N-glycosidase F.

50 after deglycosylation with endoglycosidase F/peptide-N-glycosidase F.

51 in Xenopus oocytes and deglycosylation with peptide-N-glycosidase F.

52 hrough and bound fractions, and treated with peptide: N-glycosidase F to remove N-linked glycans.

53 rescent protein-tagged mCLCA6 with PNGase F (peptide: N-glycosidase F) to remove N-linked glycosyl gr

54 sing endo-beta-N-acetylglucosaminidase H and peptide:N-glycosidase F sensitivity assays on CDKAL1 con

55 proximately 120 kDa following treatment with peptide:N-glycosidase F, consistent with N-glycosylation

56 ological approaches, including use of either peptide:N-glycosidases F and A (PNGase F and A) or anhyd

57 purified enzyme could be deglycosylated with peptide N-glycosidase-F to a core molecular mass of 54 k

58 osylation of the full-length homotrimer with peptide N-glycosidase-F under native conditions abolishe

59 teins using sequential endoglycosidase H and peptide:N-glycosidase-F digestions.

60 ds were comparable to profiling by PNGase F (peptide N-glycosidase from Flavobacterium meningosepticu

61 [35S]methionine labeling of BRL-3A cells, a peptide:N-glycosidase-sensitive protein (45 kDa) was obs

62 Endoglycosidase H and peptide N-glycosidase treatment and cell surface immunop