ライフサイエンスコーパス: peptide map

1 ernible singly charged ions detected in a MS peptide map.

2 g 32P-labeled peptides isolated from tryptic peptide maps.

3 difficult to detect them in the conventional peptide maps.

4 extracted ion chromatograms from the tryptic peptide maps.

5 n vivo by [(32)P]orthophosphate labeling and peptide mapping.

6 ion by mass spectrometry and two-dimensional peptide mapping.

7 2-amino acid motif in the N-terminal CbpA by peptide mapping.

8 tion signals as determined by phosphotryptic peptide mapping.

9 ool to increase sequence coverage in tryptic peptide mapping.

10 esis-electrospray ionization (CE-ESI)-TOF MS peptide mapping.

11 spectrometry, ion mobility, and quantitative peptide mapping.

12 by peptide sequencing and mass spectrometric peptide mapping.

13 residue reactivity with dithiodipyridine and peptide mapping.

14 ing to the partial amino acid sequences from peptide mapping.

15 n, followed by proteolytic and immunological peptide mapping.

16 and liquid chromatography/mass spectrography peptide mapping.

17 of DNA-dependent protein kinase (DNA-PK) by peptide mapping.

18 ee-disulfide species have been identified by peptide mapping.

19 graphy, and PTM levels were calculated using peptide mapping.

20 spectrometry and glycation sites located by peptide mapping.

21 ein variants that are difficult to detect by peptide mapping.

22 ing truncated GBS-PGK molecules, followed by peptide mapping.

23 ation was investigated by mass spectrometric peptide mapping.

24 uid chromatography-mass spectrometry (LC-MS) peptide mapping.

25 residue preceding lysine 222, determined by peptide mapping.

26 MAP-D002 peptide was higher than that of the peptide MAP-911, whereas the avidity of D002 peptide was

27 e we examined the effect of mouse activating peptide (mAP), a specific activator of PAR-2, on electro

28 y mass (10000.5 Da) and amino acid sequence (peptide mapping after proteolysis) determined by matrix-

29 n sites of carboxylase by mass spectrometric peptide mapping analyses combined with site-directed mut

30 l and complementary technique to RPLC-MS for peptide mapping analyses of antibody-drug conjugates (AD

31 We report the use of peptide map analysis by reversed-phase high-performance

32 to demonstrate the utility in improving both peptide mapping analysis and intact mass analysis, the t

33 ll ~25 kDa fragments, followed by an on-line peptide mapping analysis of each fragment.

34 reduced by 60, 7, and 96%, respectively, and peptide mapping analysis of the mutant enzymes confirmed

35 as a result of molecular mass determination, peptide mapping analysis, and MS/MS sequencing.

36 ered as a result of intact mass measurement, peptide mapping analysis, and tandem mass spectroscopy s

37 Following peptide mapping analysis, significant amounts of asparty

38 ization on the isolated glycated material by peptide mapping analysis, using liquid chromatography-ma

39 Detailed tryptic peptide mapping analysis, using MALDI/TOF-MS, identified

40 idation, and succinimide formation by online peptide mapping analysis.

41 etween proteolytic peptide masses from an MS peptide map and theoretical proteolytic peptide masses o

42 Bottom-up characterization using RP-HPLC/MS peptide mapping and accurate mass measurements identifie

43 and the antigenic structure of B5R(275t) by peptide mapping and by reciprocal MAb blocking studies u

44 rimary structure of mefp-5 was determined by peptide mapping and cDNA sequencing.

45 nized with HCV-1 rE1E2 was conducted through peptide mapping and competition studies with a panel of

46 of high mass accuracy in mass spectrometric peptide mapping and database searching, selected protein

47 We used peptide mapping and epitope-specific immunoprecipitation

48 Subsequent trapping, proteolysis, peptide mapping and fragmentation by mass spectrometry,

49 MS proteolytic peptide mapping and genome database searching provide a

50 ed laser desorption-ionization (MALDI) TOFMS peptide mapping and intact MW so that a standard map is

51 Tryptic peptide mapping and MALDI-MS verify labeling at the engi

52 -inactivated CYP3A4(His)(6) followed by HPLC-peptide mapping and mass spectrometric (LC/MS/MS) analys

53 tion of various deamidated forms followed by peptide mapping and mass spectrometric analyses revealed

54 By peptide mapping and mass spectrometric analysis of a sol

55 Preliminary peptide mapping and mass spectrometry analysis suggest t

56 direct phosphorylation in vitro followed by peptide mapping and mass spectrometry sequencing.

57 Peptide mapping and matrix-assisted laser desorption ion

58 ported by quantitative results acquired from peptide mapping and methylamine labeling.

59 using the traditional bottom-up approach of peptide mapping and MS sequencing methodologies, two DMP

60 Peptide mapping and mutagenesis studies have also shown

61 vitro by ERK1, JNK and p38, and confirmed by peptide mapping and mutagenesis that Thr53 is phosphoryl

62 Using tryptic peptide mapping and mutagenesis, we have identified seri

63 e element of the GLUT1 ATP binding domain by peptide mapping and N-terminal sequence analysis of prot

64 Mass spectrometry-based peptide mapping and N-terminal sequencing demonstrated t

65 Further peptide mapping and sequence analysis of CB11 revealed e

66 Peptide mapping and sequencing data indicate that the pa

67 We report the first peptide mapping and sequencing of an in vivo isolevuglan

68 We used two-dimensional peptide mapping and sequencing to identify three residue

69 Furthermore, by peptide mapping and site-directed mutagenesis we demonst

70 Using peptide mapping and site-directed mutagenesis, we have i

71 Peptide mapping and structural analysis by Fourier trans

72 Peptide mapping and subsequent mass spectrometry analysi

73 Tryptic peptide mapping and tandem mass sequencing were used to

74 Tryptic peptide mapping and tandem mass spectrometry of the redu

75 By combining proteolytic digestion with peptide mapping and tandem mass spectrometry techniques,

76 aphy, were fully characterized using tryptic peptide mapping and tandem mass spectrometry.

77 ction/alkylation of the protein, followed by peptide mapping and tanden mass spectrometry (MS/MS) seq

78 ved and homogeneous, which was determined by peptide mapping and top-down analysis of PE, PilA, and P

79 The data generated, including peptide maps and tandem MS spectra, were analyzed using

80 parameter together with the intact MW value, peptide map, and MS/MS results for identification of the

81 Immunoreactive epitopes were searched for by peptide mapping, and 171 cleavable, biotinylated 17-mer

82 irected mutagenesis, chemical cross-linking, peptide mapping, and LC-MS/MS analyses.

83 complex with trypsin, followed by isolation, peptide mapping, and mass spectrometric and tandem mass

84 ies of VEGF-D using a neutralizing antibody, peptide mapping, and mutagenesis to demonstrate that the

85 se serines, based on in vitro kinase assays, peptide mapping, and mutational analysis.

86 Using a combination of receptor mutagenesis, peptide mapping, and N-terminal sequencing, we identifie

87 (ESI-q-TOF-MS), N-terminal Edman sequencing, peptide mapping, and other techniques.

88 with monoclonal antibodies, one-dimensional peptide mapping, and partial amino acid sequencing demon

89 were identified by purification, proteolytic peptide mapping, and radiochemical sequencing of labeled

90 by absorbance and fluorescence spectroscopy, peptide mapping, and sequence analysis.

91 The multiple antigen peptide (MAP) approach is an effective method to chemica

92 nces within EC2 and N terminus identified by peptide mapping are in close proximity in the equilibriu

93 Accurate mass (+/-0.010 Da) peptide maps are generated from a standard six-protein d

94 n-labeled RTPR with endoproteinase Glu-C and peptide mapping at pH 5.8 revealed that C419 was predomi

95 inhibitor could no longer be detected after peptide mapping at this site or at the catalytic site.

96 rating peptide (CPP), i.e. Model Amphipathic Peptide (MAP), attached to a pH-sensitive masking peptid

97 orm identification by our laboratory using a peptide map-based method.

98 Two synthetic peptides mapping basic clusters of the cytosolic sClC3-C

99 Peptide mapping benefits from an efficient front-end sep

100 tics of the ABX and rituximab in AR160 using peptide mapping/Biacore approach.

101 Peptide mapping by high performance liquid chromatograph

102 eins were digested with cyanogen bromide and peptide mapping by LC-MS was established.

103 Peptide mapping by matrix-assisted laser desorption/ioni

104 gel electrophoresis with in-gel proteolysis, peptide mapping by MS, and sequence database searches fo

105 of cross-linking were determined by tryptic peptide mapping by using time-of-flight MS.

106 d by MS are not unique; i.e., each mass in a peptide map can match randomly one or several proteins i

107 data obtained demonstrate that MAM by LC-MS peptide mapping can, in principle, adequately replace se

108 he potential to be applied under the general peptide mapping conditions.

109 e 50-kDa gingipain R2, or multiple antigenic peptide (MAP)-conjugated gingipain R-derived peptides an

110 size and fully characterize multiple antigen peptide (MAP) conjugates containing protective epitopes

111 ve efficacy in rabbits of multiple antigenic peptides (MAPs) consisting of aa 304-319 from the LND of

112 mice with three doses of a multiple antigen peptide (MAP) construct containing four branches of amin

113 Multiple antigenic peptide (MAP) constructs were synthesized from promising

114 oped an approach of lipidating a multiple Ag peptide (MAP) containing part of the V3 loop from HIV-1

115 Multiple Ag peptides (MAPs) containing eight proteolipid protein (PL

116 d fluorescence detection, the sensitivity of peptide mapping could be improved 2000 times compared to

117 Peptide mapping coupled with liquid chromatography-mass

118 Peptide mapping coupled with mass spectrometric analyses

119 d after limited proteolysis was confirmed by peptide mapping coupled with tandem mass spectrometry an

120 intact and reduced mass analysis as well as peptide map data derived from the three major HIC fracti

121 Subsequent tryptic and chymotryptic peptide map data indicated that the second glycosylation

122 m protein databases using mass spectrometric peptide mapping data.

123 MHC class I-associated peptides (MAPs) define the immune self for CD8+ T lympho

124 p (Glu(345)), as demonstrated by proteolytic peptide mapping, deglycosylation, micropurification, and

125 Peptide mapping demonstrated that both TGF-beta 1 and p3

126 Here we describe a simple approach to peptide mapping designed for large sample sets that incl

127 Peptide mapping determined a minimum of five conserved e

128 he liquid phase and the molecular weight and peptide maps determined by MALDI-TOF MS for identificati

129 with chemically modified, multiple antigenic peptides (MAPs) developed for the detection and discrimi

130 previously showed that a multiple antigenic peptide (MAP) displaying amino acids (aa) 305 to 319 fro

131 ogenicity in rabbits of a multiple antigenic peptide (MAP) displaying copies of amino acids (aa) 305

132 pectrometry (CZE-MS) has great potential for peptide mapping due to high efficiency and outstanding s

133 ressed in COS-1 cells using a combination of peptide mapping, Edman degradation, and mass spectrometr

134 ve approach for protein characterization via peptide mapping employing a data independent LC-MS acqui

135 as well as other information relevant to the peptide mapping experiment.

136 Peptide mapping experiments identified the mAb 2E1 cross

137 Two-dimensional peptide mapping experiments showed that one of the Cx45.

138 Our peptide mapping experiments showed that the Ser(363) sit

139 However, despite this considerable homology, peptide-mapping experiments also revealed that immunodom

140 munodepletion, in vitro phosphorylation, and peptide-mapping experiments indicated that Cdc2 is likel

141 Peptide mapping followed by sequence analysis revealed t

142 s labeled with lutetium-177 and subjected to peptide mapping followed by sequence analysis.

143 include CE as a complement to reverse-phase peptide mapping for the identification of small peptides

144 tion of the analytical artifact during LC-MS peptide mapping for the measurement of Met sulfoxide.

145 ns in linear MALDI/MS and was reconfirmed by peptide mapping for three of the proteins.

146 cts were synthesized in a multiple antigenic peptide (MAP) format.

147 ncanavalin A presented in a multiple antigen peptide (MAP) format.

148 Comparison of peptide maps from in vivo and in vitro phosphorylated be

149 ined by simulations utilizing random tryptic peptide maps generated from a genome database.

150 on-exchange chromatography (CEX) and tryptic peptide maps generated with the new digestion method.

151 Although mass spectrometric peptide mapping has become an established technique for

152 labeling, trypsin digestion, two-dimensional peptide mapping, high performance liquid chromatography,

153 A tryptic peptide map identified two isoAsp-containing peptides, b

154 ct association between the two proteins, and peptide mapping identified an ERK2 binding site within t

155 Mass spectrometry peptide mapping identified arginine-18 as the hotspot si

156 Peptide mapping identified one disulfide bond between Cy

157 Peptide mapping identified two acidic clusters in DMP1 r

158 s the determination of such heterogeneity by peptide mapping in both the heavy chain and the light ch

159 Peptide mapping in conjunction with mass spectrometry sh

160 as further complemented experimentally using peptide mapping in tandem with mass spectrometry and sit

161 artic acid and 80% aspartic acid detected by peptide mapping in the degraded sample (8 weeks, 45 degr

162 dified minimally with methylglyoxal, tryptic peptide mapping indicated a hotspot of modification at A

163 Peptide mapping is a useful technique for identifying po

164 The most commonly used method for peptide mapping is based on reverse phase liquid chromat

165 Liquid chromatography (LC)-based peptide mapping is extensively used for establishing pro

166 omatography with mass spectrometry (RPLC-MS) peptide mapping is routinely used for interrogating mole

167 LC-MS/MS peptide mapping is well suited to the discovery and quan

168 ng, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical re

169 The automated peptide mapping LC/MS system has great utility in prepar

170 ional and chemical modifications in a single peptide mapping liquid chromatography-mass spectrometry

171 A completely automated peptide mapping liquid chromatography/mass spectrometry

172 A multiple antigen peptide (MAP) malaria vaccine containing minimal Plasmod

173 Peptide mapping mass spectrometry indicated that host-sp

174 The tryptic peptide map (mass spectral analysis) revealed that the G

175 ied with fucose and through a combination of peptide mapping, mass spectrometry, and sequence analysi

176 In the work reported here, we used peptide mapping, mass spectrometry, and site-directed mu

177 Here we used peptide mapping, mass spectroscopy analysis, and mutagen

178 rt the development of an improved nonreduced peptide map method coupled with machine learning to enab

179 The peptide map method described here was capable of detecti

180 from trifluoroacetic acid (TFA) in a typical peptide mapping method can be effectively mitigated, thu

181 a high-resolution, high-sensitivity LC-UV-MS peptide mapping method for the therapeutic antibody, tra

182 A highly sensitive peptide mapping method using derivatization and fluoresc

183 MAM is a peptide mapping method utilizing mass spectrometry to de

184 ns accurately, an isotope labeling and LC-MS peptide mapping method was developed.

185 The CZE-MS peptide mapping method with the modified BGE produced si

186 mine, by a sodium borohydride-dependent mass peptide mapping method, the galactation sites in HSA; an

187 Both LC-MS and peptide mapping methodologies were found to be useful in

188 This study showed that the new peptide mapping methodology with a combination of mass s

189 e chromophores were further located by a new peptide mapping methodology with a combination of mass s

190 The peptide mapping methods developed for characterization a

191 However, current LC-UV/MS peptide mapping methods require multiple analyses and MS

192 lytic (lysylendopeptidase-C) digestion, HPLC-peptide mapping, microEdman sequencing, and mass spectro

193 Classical peptide mapping narrowed the major phosphorylation site

194 drug-target interaction network and protein-peptide mapping network.

195 tide observed from the reduced and alkylated peptide map of the engineered mAb.

196 Peptide mapping of >80% of the residues was accomplished

197 Peptide mapping of 3H-methyl-labeled H3 indicated methyl

198 Peptide mapping of a 20 fmol amount of tagged digest was

199 hogonal technique with growing attention for peptide mapping of biotherapeutic proteins due to its hi

200 er516 was confirmed by tryptic digestion and peptide mapping of COX-2 labeled with [1-14C-acetyl]sali

201 Tryptic digestion and peptide mapping of COX-2 reacted with [1-14C-acetyl]-36

202 By two-dimensional tryptic peptide mapping of immunoprecipitated NHE-1, we identify

203 Using two-dimensional peptide mapping of in vivo radiolabeled p53 tryptic phos

204 Peptide mapping of labeled wild-type and mutant receptor

205 Peptide mapping of mono(3'-dADP-ribosyl)ated-PARP follow

206 The system was used for peptide mapping of monoclonal antibodies (mAbs), known a

207 1) and Cys(32)-Cys(39) by protease-generated peptide mapping of partially reduced and S-alkylated rSM

208 A novel multimodal method for peptide mapping of proteins by multiplexed capillary ele

209 e-of-flight mass spectrometry (MALDI-TOF MS) peptide mapping of proteins isolated by PAGE.

210 identification of phosphopeptides from HPLC peptide mapping of proteolytic digests of phosphoprotein

211 Moreover, peptide mapping of sera from animals receiving cross-lin

212 In vitro PKA phosphorylation and tryptic peptide mapping of SNS and mutant SNS(SA) I-II loops exp

213 proved mixing experiments and by comparative peptide mapping of specific polypeptides recovered from

214 nto two subgroups based on serological data, peptide mapping of the coat protein, nucleic acid hybrid

215 Peptide mapping of the Cu2+-inactivated enzyme revealed

216 Peptide mapping of the irreversibly bound heme adduct in

217 to be proximal to the major groove of DNA by peptide mapping of the region of TBP cross-linked at bp

218 Peptide mapping of the tagged digest reviews a larger nu

219 The peptide mapping of the tagged digest was conducted with

220 Peptide mapping of the tBPA-modified protein provides ev

221 Comparative peptide mapping of these B15 allotypes further pinpoints

222 Peptide mapping of tryptic digests of the inactivated CY

223 During peptide mapping of unalkylated hemoglobins with Staphylo

224 Peptide maps of both ASCs digested by chymotrypsin and t

225 y mutations affecting Ser831 altered the 32P peptide maps of GluR1 from HEK-293 cells co-expressing a

226 TOFMS to determine the molecular weights and peptide maps of the proteins.

227 from an isolated protein followed by either peptide mapping or tandem MS (MS/MS) to obtain sequence

228 Peptides maps or sequences were used for database search

229 Consistent with the reconstructions, peptide mapping places the ubiquitin linkage on lysine 1

230 tested in the development of a miniaturized peptide map procedure.

231 haracterization of PTMs using a conventional peptide mapping procedure requires time-consuming and la

232 A new peptide mapping procedure, incorporating derivatization

233 s with this new method compared to a typical peptide mapping procedure.

234 is, and data interpretation than traditional peptide mapping procedures.

235 The optimized peptide map protocol that requires <1 mug of mAb include

236 d that they were different, with the tryptic peptide maps providing evidence that the beta-subunits l

237 g is monitored using mass spectrometry-based peptide mapping, providing spatially resolved measuremen

238 Detailed peptide mapping revealed as many as seven covalent cross

239 Peptide mapping revealed that ferritin binds to a 22-aa

240 Peptide mapping revealed that the modification occurred

241 present results from optimization of CZE-MS peptide mapping separation using mixed aqueous-aprotic d

242 The resulting proteins were characterized by peptide mapping, sequence analysis, and mass spectrometr

243 In the case of peptide mapping, several peptide masses are needed to un

244 Remarkably, peptide mapping showed most epitopes recognized by naive

245 high performance liquid chromatography, and peptide mapping showed that it was the same in the two e

246 Peptide mapping showed that the alpha beta and (alpha be

247 Tryptic peptide mapping showed that the CpcSU-dependent reaction

248 Systematic peptide mapping showed that the site of interaction betw

249 mbination with mass spectrometry and tryptic peptide mapping showed unambiguously that RLF is larger

250 with FPR are consistent with cross-linking, peptide mapping, spectroscopic, and electron transfer da

251 This approach also includes a differential peptide mapping step for identification of pyroglutamate

252 Peptide mapping studies covering 88% of the deduced amin

253 Previous epitope and peptide mapping studies have also indicated that the PIL

254 Southern blot and peptide mapping studies indicated that this 31-kDa antig

255 Peptide mapping studies of in vivo phosphorylated TXA2 r

256 Peptide mapping studies revealed that more positive reca

257 sult, taken together with the results of the peptide mapping studies, establishes that the site of Bp

258 rroborated the chemical modification and the peptide mapping studies, establishing the importance of

259 Peptide-mapping studies of IgG subclass responses identi

260 The multiple antigenic peptide (MAP) system is a well-known example of a discre

261 as a function of the number of masses in the peptide map, the mass accuracy, the degree of incomplete

262 Peptide mapping, thiol titrations, UV-vis spectrophotome

263 This dimerization interface is validated by peptide mapping through hydrogen/deuterium exchange mass

264 used radioactive iodide labeling followed by peptide mapping to gain insight into the structure of P.

265 es, we used photo affinity cross-linking and peptide mapping to identify the substrate-binding sites

266 Ultimately, the proteins can be studied by peptide mapping to search for posttranslational modifica

267 In this study, we have used peptide mapping to study the oxidation kinetics of each

268 Some transcytosis-selected peptides map to the same 402-410 pIgR-binding Calpha3 si

269 performed and demonstrated that 13 different peptides mapped to identical regions of MchA1 and MchA2.

270 Only 5 of the 31 broadly reactive Th epitope peptides mapped to the surface (SU) domain of Env.

271 Peptides mapping to 2784 proteins in 1168 protein groups

272 sequence coverage by the number of distinct peptides mapping to each protein identification, the CIT

273 ssays was demonstrated against, or shown by, peptides mapping to the third and fourth predicted surfa

274 as desorbed, digested using trypsin, and the peptide mapped using LC/MS.

275 Subsequent peptide mapping using chemical and proteinase cleavages

276 e Ser/Thr kinase domain of PKCdelta based on peptide mapping using liquid chromatography/mass spectro

277 as identified as the phosphorylation site by peptide mapping using mass spectrometry, site-directed m

278 nium-containing fractions were identified by peptide mapping using nano LC-ESI/LTQMS.

279 Peptide mapping using Urea-PAGE followed by CA revealed

280 accurate molecular weight together with the peptide map was used to obtain protein identification us

281 Peptide mapping was performed using high-resolution MS,

282 Using two-dimensional peptide mapping, we demonstrate that peptides correspond

283 Using deletion mutagenesis and peptide mapping, we have identified the sequences in Cdc

284 study, mass spectrometry and two-dimensional peptide mapping were used to determine that tyrosines 22

285 sted samples, which limits the efficiency of peptide mapping when there is an increasing demand for p

286 ntages were evaluated through application to peptide mapping, wherein CSH C18 was found to aid the de

287 raction were counted for 3H label within the peptide map which rapidly pinpointed the original four p

288 e alternative to conventional time-intensive peptide mapping which is prone to artificial oxidation d

289 with Girard's Reagent T (GRT) and subsequent peptide mapping with high-resolution mass spectrometry.

290 Peptide mapping with liquid chromatography-tandem mass s

291 Peptide mapping with mass spectrometry (MS) detection is

292 18O, and time point samples were analyzed by peptide mapping with mass spectrometry to measure the ra

293 a8 was isolated by column chromatography for peptide mapping with mass spectrometry.

294 pwise reduction and alkylation at acidic pH, peptide mapping with matrix-assisted laser desorption io

295 Its structure was elucidated by peptide mapping with multiple proteases with various spe

296 Currently, peptide mapping with reversed-phase liquid chromatograph

297 lonal antibody by LC-MS and nonreduced Lys-C peptide mapping with tandem mass spectrometry.

298 ing on the beta1 chain was localized by CNBr peptide mapping within residues 130-146, a region that c

299 s method adds just one step to the classical peptide mapping workflow.

300 phosphoserine as the putative PKA site, and peptide mapping yielded one phosphopeptide.