ライフサイエンスコーパス: peptide mapping

1 2-amino acid motif in the N-terminal CbpA by peptide mapping.

2 tion signals as determined by phosphotryptic peptide mapping.

3 ool to increase sequence coverage in tryptic peptide mapping.

4 esis-electrospray ionization (CE-ESI)-TOF MS peptide mapping.

5 spectrometry, ion mobility, and quantitative peptide mapping.

6 by peptide sequencing and mass spectrometric peptide mapping.

7 residue reactivity with dithiodipyridine and peptide mapping.

8 ing to the partial amino acid sequences from peptide mapping.

9 n, followed by proteolytic and immunological peptide mapping.

10 and liquid chromatography/mass spectrography peptide mapping.

11 of DNA-dependent protein kinase (DNA-PK) by peptide mapping.

12 ee-disulfide species have been identified by peptide mapping.

13 gelsolin by amino acid sequencing following peptide mapping.

14 ug) of intact antibody, without the need for peptide mapping.

15 automated online protein digestion and LC-MS peptide mapping.

16 nder stress conditions were characterized by peptide mapping.

17 graphy, and PTM levels were calculated using peptide mapping.

18 spectrometry and glycation sites located by peptide mapping.

19 ein variants that are difficult to detect by peptide mapping.

20 ing truncated GBS-PGK molecules, followed by peptide mapping.

21 ation was investigated by mass spectrometric peptide mapping.

22 uid chromatography-mass spectrometry (LC-MS) peptide mapping.

23 residue preceding lysine 222, determined by peptide mapping.

24 n vivo by [(32)P]orthophosphate labeling and peptide mapping.

25 ion by mass spectrometry and two-dimensional peptide mapping.

26 ve-like conformation was further verified by peptide mapping after limited trypsin proteolysis, and b

27 y mass (10000.5 Da) and amino acid sequence (peptide mapping after proteolysis) determined by matrix-

28 n sites of carboxylase by mass spectrometric peptide mapping analyses combined with site-directed mut

29 l and complementary technique to RPLC-MS for peptide mapping analyses of antibody-drug conjugates (AD

30 to demonstrate the utility in improving both peptide mapping analysis and intact mass analysis, the t

31 tified as casein kinase Ialpha (CKIalpha) by peptide mapping analysis and sequencing.

32 ll ~25 kDa fragments, followed by an on-line peptide mapping analysis of each fragment.

33 reduced by 60, 7, and 96%, respectively, and peptide mapping analysis of the mutant enzymes confirmed

34 as a result of molecular mass determination, peptide mapping analysis, and MS/MS sequencing.

35 ered as a result of intact mass measurement, peptide mapping analysis, and tandem mass spectroscopy s

36 hich relied on either untargeted or targeted peptide mapping analysis, could lead to incomplete ident

37 Following peptide mapping analysis, significant amounts of asparty

38 ization on the isolated glycated material by peptide mapping analysis, using liquid chromatography-ma

39 Detailed tryptic peptide mapping analysis, using MALDI/TOF-MS, identified

40 lumn digestion setup, followed by a combined peptide mapping analysis.

41 e chromatography and subsequent online LC-MS peptide mapping analysis.

42 idation, and succinimide formation by online peptide mapping analysis.

43 ns) were phosphorylated in vivo, and tryptic peptide-mapping analysis suggested a single, similar pho

44 Bottom-up characterization using RP-HPLC/MS peptide mapping and accurate mass measurements identifie

45 ication was determined to be Tyr-236 by CNBr peptide mapping and automated peptide sequencing.

46 multi-attribute method (MAM), based on LC-MS peptide mapping and automation principles, can be used t

47 and the antigenic structure of B5R(275t) by peptide mapping and by reciprocal MAb blocking studies u

48 rimary structure of mefp-5 was determined by peptide mapping and cDNA sequencing.

49 nized with HCV-1 rE1E2 was conducted through peptide mapping and competition studies with a panel of

50 aces required for SARM1 autoinhibition using peptide mapping and cryo-electron microscopy (cryo-EM).

51 of high mass accuracy in mass spectrometric peptide mapping and database searching, selected protein

52 We used peptide mapping and epitope-specific immunoprecipitation

53 Subsequent trapping, proteolysis, peptide mapping and fragmentation by mass spectrometry,

54 MS proteolytic peptide mapping and genome database searching provide a

55 ed laser desorption-ionization (MALDI) TOFMS peptide mapping and intact MW so that a standard map is

56 Tryptic peptide mapping and MALDI-MS verify labeling at the engi

57 was demonstrated by two-dimensional tryptic peptide mapping and mass analysis to be either threonine

58 -inactivated CYP3A4(His)(6) followed by HPLC-peptide mapping and mass spectrometric (LC/MS/MS) analys

59 tion of various deamidated forms followed by peptide mapping and mass spectrometric analyses revealed

60 By peptide mapping and mass spectrometric analysis of a sol

61 Preliminary peptide mapping and mass spectrometry analysis suggest t

62 direct phosphorylation in vitro followed by peptide mapping and mass spectrometry sequencing.

63 Peptide mapping and matrix-assisted laser desorption ion

64 ported by quantitative results acquired from peptide mapping and methylamine labeling.

65 using the traditional bottom-up approach of peptide mapping and MS sequencing methodologies, two DMP

66 Peptide mapping and mutagenesis studies have also shown

67 vitro by ERK1, JNK and p38, and confirmed by peptide mapping and mutagenesis that Thr53 is phosphoryl

68 Using tryptic peptide mapping and mutagenesis, we have identified seri

69 Peptide mapping and mutational analyses localized the bu

70 e element of the GLUT1 ATP binding domain by peptide mapping and N-terminal sequence analysis of prot

71 Mass spectrometry-based peptide mapping and N-terminal sequencing demonstrated t

72 Peptide mapping and N-terminal sequencing has been used

73 Peptide mapping and phosphatase diagests showed that the

74 Further peptide mapping and sequence analysis of CB11 revealed e

75 Peptide mapping and sequencing data indicate that the pa

76 We report the first peptide mapping and sequencing of an in vivo isolevuglan

77 We used two-dimensional peptide mapping and sequencing to identify three residue

78 Furthermore, by peptide mapping and site-directed mutagenesis we demonst

79 Using peptide mapping and site-directed mutagenesis, we have i

80 Peptide mapping and structural analysis by Fourier trans

81 Peptide mapping and subsequent mass spectrometry analysi

82 Tryptic peptide mapping and tandem mass sequencing were used to

83 Tryptic peptide mapping and tandem mass spectrometry of the redu

84 By combining proteolytic digestion with peptide mapping and tandem mass spectrometry techniques,

85 aphy, were fully characterized using tryptic peptide mapping and tandem mass spectrometry.

86 ction/alkylation of the protein, followed by peptide mapping and tanden mass spectrometry (MS/MS) seq

87 ved and homogeneous, which was determined by peptide mapping and top-down analysis of PE, PilA, and P

88 Immunoreactive epitopes were searched for by peptide mapping, and 171 cleavable, biotinylated 17-mer

89 irected mutagenesis, chemical cross-linking, peptide mapping, and LC-MS/MS analyses.

90 complex with trypsin, followed by isolation, peptide mapping, and mass spectrometric and tandem mass

91 mance liquid chromatography, two-dimensional peptide mapping, and matrix-assisted laser desorption/io

92 ies of VEGF-D using a neutralizing antibody, peptide mapping, and mutagenesis to demonstrate that the

93 se serines, based on in vitro kinase assays, peptide mapping, and mutational analysis.

94 Using a combination of receptor mutagenesis, peptide mapping, and N-terminal sequencing, we identifie

95 (ESI-q-TOF-MS), N-terminal Edman sequencing, peptide mapping, and other techniques.

96 with monoclonal antibodies, one-dimensional peptide mapping, and partial amino acid sequencing demon

97 so subjected to proteolysis, two-dimensional peptide mapping, and phosphoamino acid analysis.

98 were identified by purification, proteolytic peptide mapping, and radiochemical sequencing of labeled

99 by absorbance and fluorescence spectroscopy, peptide mapping, and sequence analysis.

100 ar to results obtained using high-resolution peptide mapping, and the method was robust and reproduci

101 nces within EC2 and N terminus identified by peptide mapping are in close proximity in the equilibriu

102 ction of activated B-cells was identified by peptide mapping as alpha-tubulin.

103 n-labeled RTPR with endoproteinase Glu-C and peptide mapping at pH 5.8 revealed that C419 was predomi

104 inhibitor could no longer be detected after peptide mapping at this site or at the catalytic site.

105 By peptide mapping, automated sequencing, and mass spectrom

106 In this study, we present an optimized peptide mapping-based workflow that provides thorough ch

107 Two synthetic peptides mapping basic clusters of the cytosolic sClC3-C

108 Peptide mapping benefits from an efficient front-end sep

109 tics of the ABX and rituximab in AR160 using peptide mapping/Biacore approach.

110 Peptide mapping by high performance liquid chromatograph

111 We used peptide mapping by HPLC, Edman sequencing, and matrix-as

112 eins were digested with cyanogen bromide and peptide mapping by LC-MS was established.

113 Peptide mapping by matrix-assisted laser desorption/ioni

114 gel electrophoresis with in-gel proteolysis, peptide mapping by MS, and sequence database searches fo

115 of cross-linking were determined by tryptic peptide mapping by using time-of-flight MS.

116 data obtained demonstrate that MAM by LC-MS peptide mapping can, in principle, adequately replace se

117 he potential to be applied under the general peptide mapping conditions.

118 d fluorescence detection, the sensitivity of peptide mapping could be improved 2000 times compared to

119 Peptide mapping coupled with liquid chromatography-mass

120 Peptide mapping coupled with mass spectrometric analyses

121 d after limited proteolysis was confirmed by peptide mapping coupled with tandem mass spectrometry an

122 HPLC-ESI-MS peptide mapping data demonstrated that the purified muri

123 m protein databases using mass spectrometric peptide mapping data.

124 p (Glu(345)), as demonstrated by proteolytic peptide mapping, deglycosylation, micropurification, and

125 Peptide mapping demonstrated that both TGF-beta 1 and p3

126 Here we describe a simple approach to peptide mapping designed for large sample sets that incl

127 Peptide mapping determined a minimum of five conserved e

128 pectrometry (CZE-MS) has great potential for peptide mapping due to high efficiency and outstanding s

129 ressed in COS-1 cells using a combination of peptide mapping, Edman degradation, and mass spectrometr

130 lytical methods such as amino acid analysis, peptide mapping, electrospray mass spectrometry, and Edm

131 ve approach for protein characterization via peptide mapping employing a data independent LC-MS acqui

132 as well as other information relevant to the peptide mapping experiment.

133 Peptide mapping experiments identified the mAb 2E1 cross

134 Two-dimensional peptide mapping experiments showed that one of the Cx45.

135 Our peptide mapping experiments showed that the Ser(363) sit

136 However, despite this considerable homology, peptide-mapping experiments also revealed that immunodom

137 munodepletion, in vitro phosphorylation, and peptide-mapping experiments indicated that Cdc2 is likel

138 Peptide mapping followed by sequence analysis revealed t

139 s labeled with lutetium-177 and subjected to peptide mapping followed by sequence analysis.

140 include CE as a complement to reverse-phase peptide mapping for the identification of small peptides

141 tion of the analytical artifact during LC-MS peptide mapping for the measurement of Met sulfoxide.

142 ns in linear MALDI/MS and was reconfirmed by peptide mapping for three of the proteins.

143 Although mass spectrometric peptide mapping has become an established technique for

144 labeling, trypsin digestion, two-dimensional peptide mapping, high performance liquid chromatography,

145 LC-MS based peptide mapping, i.e., proteolytic digestion followed by

146 ct association between the two proteins, and peptide mapping identified an ERK2 binding site within t

147 Mass spectrometry peptide mapping identified arginine-18 as the hotspot si

148 Peptide mapping identified one disulfide bond between Cy

149 Peptide mapping identified two acidic clusters in DMP1 r

150 s the determination of such heterogeneity by peptide mapping in both the heavy chain and the light ch

151 Peptide mapping in conjunction with mass spectrometry sh

152 as further complemented experimentally using peptide mapping in tandem with mass spectrometry and sit

153 structure of the receptor was determined by peptide mapping in the absence and presence of reducing

154 artic acid and 80% aspartic acid detected by peptide mapping in the degraded sample (8 weeks, 45 degr

155 dified minimally with methylglyoxal, tryptic peptide mapping indicated a hotspot of modification at A

156 Peptide mapping is a useful technique for identifying po

157 The most commonly used method for peptide mapping is based on reverse phase liquid chromat

158 Liquid chromatography (LC)-based peptide mapping is extensively used for establishing pro

159 omatography with mass spectrometry (RPLC-MS) peptide mapping is routinely used for interrogating mole

160 However, conventional peptide mapping is time-consuming and has proven difficu

161 LC-MS/MS peptide mapping is well suited to the discovery and quan

162 ng, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical re

163 The automated peptide mapping LC/MS system has great utility in prepar

164 ional and chemical modifications in a single peptide mapping liquid chromatography-mass spectrometry

165 ther orthogonal analytical methods; however, peptide mapping liquid chromatography-tandem mass spectr

166 A completely automated peptide mapping liquid chromatography/mass spectrometry

167 Peptide mapping mass spectrometry indicated that host-sp

168 ied with fucose and through a combination of peptide mapping, mass spectrometry, and sequence analysi

169 In the work reported here, we used peptide mapping, mass spectrometry, and site-directed mu

170 Here we used peptide mapping, mass spectroscopy analysis, and mutagen

171 from trifluoroacetic acid (TFA) in a typical peptide mapping method can be effectively mitigated, thu

172 a high-resolution, high-sensitivity LC-UV-MS peptide mapping method for the therapeutic antibody, tra

173 A highly sensitive peptide mapping method using derivatization and fluoresc

174 MAM is a peptide mapping method utilizing mass spectrometry to de

175 As a part of this workflow, a tryptic peptide mapping method was also developed for accurate d

176 ns accurately, an isotope labeling and LC-MS peptide mapping method was developed.

177 The CZE-MS peptide mapping method with the modified BGE produced si

178 mine, by a sodium borohydride-dependent mass peptide mapping method, the galactation sites in HSA; an

179 sufficiently validated protein sequencing or peptide mapping method.

180 Both LC-MS and peptide mapping methodologies were found to be useful in

181 This study showed that the new peptide mapping methodology with a combination of mass s

182 e chromophores were further located by a new peptide mapping methodology with a combination of mass s

183 The peptide mapping methods developed for characterization a

184 However, current LC-UV/MS peptide mapping methods require multiple analyses and MS

185 However, operating peptide mapping methods with high-resolution mass spectr

186 ific modification with 4-vinylpyridine, HPLC peptide mapping methods, and mass spectrometry to analyz

187 lytic (lysylendopeptidase-C) digestion, HPLC-peptide mapping, microEdman sequencing, and mass spectro

188 Classical peptide mapping narrowed the major phosphorylation site

189 drug-target interaction network and protein-peptide mapping network.

190 Peptide mapping of >80% of the residues was accomplished

191 Peptide mapping of 3H-methyl-labeled H3 indicated methyl

192 Peptide mapping of a 20 fmol amount of tagged digest was

193 s regions to the immune system was tested by peptide mapping of antiserum specificities against sets

194 In the peptide mapping of biopharmaceuticals, where in-column m

195 hogonal technique with growing attention for peptide mapping of biotherapeutic proteins due to its hi

196 er516 was confirmed by tryptic digestion and peptide mapping of COX-2 labeled with [1-14C-acetyl]sali

197 Tryptic digestion and peptide mapping of COX-2 reacted with [1-14C-acetyl]-36

198 By two-dimensional tryptic peptide mapping of immunoprecipitated NHE-1, we identify

199 Tryptic peptide mapping of in vivo labeled IRS-1 and the S612A m

200 Using two-dimensional peptide mapping of in vivo radiolabeled p53 tryptic phos

201 Peptide mapping of labeled wild-type and mutant receptor

202 Peptide mapping of mono(3'-dADP-ribosyl)ated-PARP follow

203 The system was used for peptide mapping of monoclonal antibodies (mAbs), known a

204 1) and Cys(32)-Cys(39) by protease-generated peptide mapping of partially reduced and S-alkylated rSM

205 A novel multimodal method for peptide mapping of proteins by multiplexed capillary ele

206 e-of-flight mass spectrometry (MALDI-TOF MS) peptide mapping of proteins isolated by PAGE.

207 identification of phosphopeptides from HPLC peptide mapping of proteolytic digests of phosphoprotein

208 Moreover, peptide mapping of sera from animals receiving cross-lin

209 In vitro PKA phosphorylation and tryptic peptide mapping of SNS and mutant SNS(SA) I-II loops exp

210 proved mixing experiments and by comparative peptide mapping of specific polypeptides recovered from

211 nto two subgroups based on serological data, peptide mapping of the coat protein, nucleic acid hybrid

212 Peptide mapping of the Cu2+-inactivated enzyme revealed

213 Peptide mapping of the irreversibly bound heme adduct in

214 for differences in Km and thermal stability, peptide mapping of the LDH-As of all six species was fir

215 to be proximal to the major groove of DNA by peptide mapping of the region of TBP cross-linked at bp

216 Peptide mapping of the tagged digest reviews a larger nu

217 The peptide mapping of the tagged digest was conducted with

218 Peptide mapping of the tBPA-modified protein provides ev

219 Comparative peptide mapping of these B15 allotypes further pinpoints

220 Peptide mapping of tryptic digests of the inactivated CY

221 Using peptide mapping of tryptic digests, LC/MS, and amino aci

222 During peptide mapping of unalkylated hemoglobins with Staphylo

223 Protein microsequencing and peptide mapping of wild-type and mutant fusion proteins

224 ble to the reported 83% coverage achieved by peptide mapping on the same ADC (Luo et al.

225 from an isolated protein followed by either peptide mapping or tandem MS (MS/MS) to obtain sequence

226 yed included various proteolytic digestions, peptide mapping, partial reduction, and assignment of di

227 Consistent with the reconstructions, peptide mapping places the ubiquitin linkage on lysine 1

228 haracterization of PTMs using a conventional peptide mapping procedure requires time-consuming and la

229 A new peptide mapping procedure, incorporating derivatization

230 s with this new method compared to a typical peptide mapping procedure.

231 is, and data interpretation than traditional peptide mapping procedures.

232 g is monitored using mass spectrometry-based peptide mapping, providing spatially resolved measuremen

233 pha 2(I) chains as determined by V8 protease peptide mapping, reached the highest intracellular level

234 Our peptide mapping results provide a more detailed structur

235 Detailed peptide mapping revealed as many as seven covalent cross

236 Peptide mapping revealed that ferritin binds to a 22-aa

237 Peptide mapping revealed that the modification occurred

238 ion of enzymatic de-N-glycosylation into the peptide mapping routine allows the N-glycan in the Fab r

239 present results from optimization of CZE-MS peptide mapping separation using mixed aqueous-aprotic d

240 The resulting proteins were characterized by peptide mapping, sequence analysis, and mass spectrometr

241 In the case of peptide mapping, several peptide masses are needed to un

242 Remarkably, peptide mapping showed most epitopes recognized by naive

243 Two-dimensional peptide mapping showed only a single phosphopeptide; two

244 dimensional gel electrophoresis and tryptic peptide mapping showed that entry into the nucleus resul

245 high performance liquid chromatography, and peptide mapping showed that it was the same in the two e

246 Peptide mapping showed that the alpha beta and (alpha be

247 Tryptic peptide mapping showed that the CpcSU-dependent reaction

248 Systematic peptide mapping showed that the site of interaction betw

249 mbination with mass spectrometry and tryptic peptide mapping showed unambiguously that RLF is larger

250 with FPR are consistent with cross-linking, peptide mapping, spectroscopic, and electron transfer da

251 This approach also includes a differential peptide mapping step for identification of pyroglutamate

252 Peptide mapping studies covering 88% of the deduced amin

253 Previous epitope and peptide mapping studies have also indicated that the PIL

254 Furthermore, peptide mapping studies indicated that BDV-P is phosphor

255 Southern blot and peptide mapping studies indicated that this 31-kDa antig

256 Peptide mapping studies of in vivo phosphorylated TXA2 r

257 Peptide mapping studies revealed that more positive reca

258 sult, taken together with the results of the peptide mapping studies, establishes that the site of Bp

259 rroborated the chemical modification and the peptide mapping studies, establishing the importance of

260 Peptide-mapping studies of IgG subclass responses identi

261 protein subunits using a high-accuracy mass peptide-mapping technique.

262 Peptide mapping, thiol titrations, UV-vis spectrophotome

263 This dimerization interface is validated by peptide mapping through hydrogen/deuterium exchange mass

264 eversed-phase (RP) column chemistry used for peptide mapping to a less retentive C4 column chemistry.

265 psilon to the gamma subunit was localized by peptide mapping to a region of the gamma subunit between

266 used radioactive iodide labeling followed by peptide mapping to gain insight into the structure of P.

267 es, we used photo affinity cross-linking and peptide mapping to identify the substrate-binding sites

268 Ultimately, the proteins can be studied by peptide mapping to search for posttranslational modifica

269 In this study, we have used peptide mapping to study the oxidation kinetics of each

270 Peptides mapping to 2784 proteins in 1168 protein groups

271 sequence coverage by the number of distinct peptides mapping to each protein identification, the CIT

272 ssays was demonstrated against, or shown by, peptides mapping to the third and fourth predicted surfa

273 Subsequent peptide mapping using chemical and proteinase cleavages

274 e Ser/Thr kinase domain of PKCdelta based on peptide mapping using liquid chromatography/mass spectro

275 as identified as the phosphorylation site by peptide mapping using mass spectrometry, site-directed m

276 nium-containing fractions were identified by peptide mapping using nano LC-ESI/LTQMS.

277 Peptide mapping using proteolytic enzymes is one useful

278 Peptide mapping using Urea-PAGE followed by CA revealed

279 Peptide mapping was performed using high-resolution MS,

280 Peptide mapping was used to identify a PKA phosphorylati

281 Using two-dimensional peptide mapping, we demonstrate that peptides correspond

282 Using deletion mutagenesis and peptide mapping, we have identified the sequences in Cdc

283 study, mass spectrometry and two-dimensional peptide mapping were used to determine that tyrosines 22

284 sted samples, which limits the efficiency of peptide mapping when there is an increasing demand for p

285 ntages were evaluated through application to peptide mapping, wherein CSH C18 was found to aid the de

286 e alternative to conventional time-intensive peptide mapping which is prone to artificial oxidation d

287 n enabled reproducible protein digestion and peptide mapping with 100% sequence coverage obtained for

288 with Girard's Reagent T (GRT) and subsequent peptide mapping with high-resolution mass spectrometry.

289 Peptide mapping with liquid chromatography-tandem mass s

290 Peptide mapping with mass spectrometry (MS) detection is

291 18O, and time point samples were analyzed by peptide mapping with mass spectrometry to measure the ra

292 a8 was isolated by column chromatography for peptide mapping with mass spectrometry.

293 pwise reduction and alkylation at acidic pH, peptide mapping with matrix-assisted laser desorption io

294 Its structure was elucidated by peptide mapping with multiple proteases with various spe

295 Currently, peptide mapping with reversed-phase liquid chromatograph

296 lonal antibody by LC-MS and nonreduced Lys-C peptide mapping with tandem mass spectrometry.

297 ing on the beta1 chain was localized by CNBr peptide mapping within residues 130-146, a region that c

298 e present a multidimensional high-throughput peptide mapping workflow that combines fast LC with cycl

299 s method adds just one step to the classical peptide mapping workflow.

300 phosphoserine as the putative PKA site, and peptide mapping yielded one phosphopeptide.