ライフサイエンスコーパス: peptide synthesis

1 linkers (originally devised for solid-phase peptide synthesis).

2 rring ligand [F(7),P(34)]-NPY by solid phase peptide synthesis.

3 oth the convergence and divergence of cyclic peptide synthesis.

4 terminus of a peptide segment by solid phase peptide synthesis.

5 have used them as units in Fmoc solid-phase peptide synthesis.

6 pounds are suitable for standard solid-phase peptide synthesis.

7 sequence, themselves obtained by solid-phase peptide synthesis.

8 fectively as melittin created by solid phase peptide synthesis.

9 analogs were synthesized through solid-phase peptide synthesis.

10 ly compatible with standard Fmoc solid-phase peptide synthesis.

11 solid phase synthesis procedures similar to peptide synthesis.

12 B and pPLB standards produced by solid-phase peptide synthesis.

13 ere generated through Fmoc-based solid phase peptide synthesis.

14 ragments, themselves prepared by solid phase peptide synthesis.

15 site-specifically using standard solid-phase peptide synthesis.

16 as both catalyst and precursor in prebiotic peptide synthesis.

17 s compatible with Fmoc-chemistry solid-phase peptide synthesis.

18 resin by conventional Fmoc-based solid-phase peptide synthesis.

19 rotected analogue for Fmoc-based solid-phase peptide synthesis.

20 is compatible with standard Fmoc solid-phase peptide synthesis.

21 ntly suppresses aspartimide formation during peptide synthesis.

22 A caspase-3/7 substrate using solution-phase peptide synthesis.

23 ccupies the active site like a substrate for peptide synthesis.

24 dinary amino acids in Fmoc-based solid-phase peptide synthesis.

25 enzymatic sulfation, chemical sulfation and peptide synthesis.

26 ployed a new linker for Fmoc-based thioester peptide synthesis.

27 that is compatible with standard Fmoc-based peptide synthesis.

28 rated into peptides via standard solid-phase peptide synthesis.

29 n domain is necessary for factor-independent peptide synthesis.

30 l inhibitors were synthesized by solid-phase peptide synthesis.

31 antly shorter and comparable to that for any peptide synthesis.

32 ted into VM(11)VVQTK by standard solid-phase peptide synthesis.

33 ucing the significant waste generated during peptide synthesis.

34 s enzymology typically found in nonribosomal peptide synthesis.

35 opriately protected for Fmoc/Boc solid-phase peptide synthesis.

36 ion and are compatible with Fmoc solid phase peptide synthesis.

37 ate, suitably protected for Fmoc solid phase peptide synthesis.

38 vitro without requiring intracellular viral peptide synthesis.

39 ized as a challenging target for solid-phase peptide synthesis.

40 thesized in a modular fashion by solid-phase peptide synthesis.

41 ta(2)-amino acids, which are useful for beta-peptide synthesis.

42 enylmethoxycarbonyl (Fmoc)-based solid-phase peptide synthesis.

43 eloped from an early system of RNA-dependent peptide synthesis.

44 lpha-C epimerization during Fmoc solid-phase peptide synthesis.

45 olid support in conjunction with solid-phase peptide synthesis.

46 N(alpha)-protection suitable for solid-phase peptide synthesis.

47 and applied to reactions used in solid-phase peptide synthesis.

48 rmediates to the various enzymes that effect peptide synthesis.

49 enylmethoxycarbonyl (Fmoc)-based solid-phase peptide synthesis.

50 ave and deprotect peptides after solid-phase peptide synthesis.

51 glycoamino acids can also serve as units for peptide synthesis.

52 s were chemically synthesized by solid-phase peptide synthesis.

53 the effect of altering the uORF sequence on peptide synthesis.

54 tance P analogue with the use of solid-phase peptide synthesis.

55 nd chemically incorporated using solid phase peptide synthesis.

56 JB3 was generated by solid phase peptide synthesis.

57 he atypically split CL intein by solid-phase peptide synthesis.

58 za B was synthesized by standard solid-phase peptide synthesis.

59 the aminoacyl-tRNA synthetases in ribosomal peptide synthesis.

60 ]-Thr-NH(2), 1) were prepared by solid-phase peptide synthesis.

61 e show here efficient 50S ribosome catalyzed peptide synthesis.

62 spectrometer was used to monitor solid-phase peptide synthesis.

63 storical development of RNA-based systems of peptide synthesis.

64 tion of TGFalpha transcription and increased peptide synthesis.

65 in I synthetase) that catalyse non-ribosomal peptide synthesis.

66 using 9-fluorenylmethoxycarbonyl solid-phase peptide synthesis.

67 e-mimicking peptides produced by solid-phase peptide synthesis.

68 specially adapted conditions of solid-phase peptide synthesis.

69 ot horizontal lineTap-ketals for solid-phase peptide synthesis.

70 ) is a strong anion byproduct of solid-phase peptide synthesis.

71 ble of sequence-specific beta-homo (beta(3)) peptide synthesis.

72 s and 33 transfer RNAs of 19 amino acids for peptide synthesis.

73 to the peptides by standard Fmoc solid phase peptide synthesis.

74 ntermediates for peptide ligation and cyclic peptide synthesis.

75 readily used in subsequent Fmoc solid-phase peptide synthesis.

76 significant enough to form aggregates during peptide synthesis.

77 cid as a building block for Fmoc solid phase peptide synthesis.

78 utility of thioacids and oxidative dimers in peptide synthesis.

79 is an attractive alternative to solid-phase peptide synthesis.

80 tion of derivatives suitable for solid phase peptide synthesis.

81 at this fragment, synthesized by solid-phase peptide synthesis, also forms fibrillar structures in wa

82 We used peptide synthesis and 2D NMR spectroscopy to assign all

83 by modification of strategies for linear aza-peptide synthesis and applied in the preparation of cycl

84 is synthesized using Fmoc-based solid-phase peptide synthesis and assembled using combined native ch

85 ave thus demonstrated that using solid phase peptide synthesis and chemical ligation it is feasible t

86 ystem to emphasize the enormous diversity in peptide synthesis and consequent complexity of the still

87 apeptide skeleton assembled via nonribosomal peptide synthesis and constrained through multiple cross

88 a 22-mer peptide, is prepared by solid-phase peptide synthesis and contains the F(n)Y at the desired

89 e been utilized in applications ranging from peptide synthesis and controlled protein activation to t

90 lated Gla domain of Factor IX by solid phase peptide synthesis and crystallized Factor IX-(1-47) in c

91 es were synthesized by solid-phase supported peptide synthesis and elongated with respective chelator

92 s new team is to highlight best practices in peptide synthesis and encourage much needed innovations.

93 ed by a tandem combination using solid phase peptide synthesis and microwave-assisted reactions.

94 Although peptide synthesis and modification are well established,

95 Peptide synthesis and MS/MS confirmed the kynurenine ass

96 lfur in the citric acid cycle, non-ribosomal peptide synthesis and polyketide biosynthesis point towa

97 tion into peptides and proteins via existing peptide synthesis and protein expression methods, we bel

98 e aim to summarize the current challenges of peptide synthesis and purification in terms of sustainab

99 se release factors is essential for accurate peptide synthesis and ribosome recycling.

100 es were successfully utilized in solid-phase peptide synthesis and semi-synthesis of histone H4.

101 Although the cytoplasmic peptide synthesis and side-chain modifications are well

102 oth the influence of cation concentration on peptide synthesis and the effect of altering the uORF se

103 LLELI[13C615N1]R was prepared by solid-phase peptide synthesis and was used as an internal standard t

104 ptides using Boc-chemistry-based solid-phase peptide synthesis, and in three of the four cases the re

105 Determination of the swelling properties, peptide synthesis, and on-bead streptavidin-alkaline pho

106 onceptually innovative approach to amide and peptide synthesis, and one that might ultimately provide

107 f biomolecules find application in ligation, peptide synthesis, and polynucleotide synthesis and sequ

108 could be recovered in up to 98% yield after peptide synthesis, and the recovered support was utilize

109 tides using Fmoc-chemistry-based solid-phase peptide synthesis, and the resulting peptides can be che

110 cation-pi binding energies using solid-phase peptide synthesis, and these analogues were modified by

111 study initiation and elongation in ribosomal peptide synthesis, and to screen for eubacterial-specifi

112 results show the power of optimized chemical peptide synthesis approaches for the efficient productio

113 Using popular terminology, what forms of peptide synthesis are appropriately termed "on-demand"?

114 and the implications for the origin of coded peptide synthesis are considered.

115 Several case studies in complex peptide synthesis are summarized here, each selected to

116 stly adopted in the solution and solid-phase peptide synthesis, are compatible to the adopted reactio

117 Translation, coded peptide synthesis, arguably exists at the heart of moder

118 odification was found to improve solid-phase peptide synthesis as demonstrated in the chemical synthe

119 , and Abeta(5-38(D23S)) by using solid phase peptide synthesis as internal standards for the CIEF imm

120 of this chemistry to reiterative solid-phase peptide synthesis as well as solid-phase fragment coupli

121 articularly convenient for hybrid alpha/beta-peptide synthesis, as demonstrated recently by our group

122 This quantitative in vitro peptide synthesis assay allowed us to analyze the abilit

123 e residues by using mutational analyses in a peptide synthesis assay with intact bacillamide syntheta

124 proline analogs require eIF5A for efficient peptide synthesis, azetidine-2-caboxylic acid, a more fl

125 These approaches include solid-phase peptide synthesis based on an adaption of native chemica

126 of genes previously implicated in interchain peptide synthesis but with unknown specific functions.

127 favor the lauroyl glycine synthesis over the peptide synthesis, but the immobilized protease has the

128 ally similar to the present-day nonribosomal peptide synthesis by multi-enzyme thiotemplate systems.

129 Endothelin-1, a peptide synthesised by vascular endothelial cells, is th

130 to climb the TR barrier in parallel with the peptide synthesis chemical step and that the nascent cha

131 s synthesized using conventional solid-phase peptide synthesis chemistry and a rhenium cyclization re

132 ze DDMs using solution phase and solid phase peptide synthesis chemistry.

133 st-generation cross-linker using solid-phase peptide synthesis chemistry.

134 acetyltransferase, and VibH, a non-ribosomal peptide synthesis condensation enzyme.

135 an be manipulated under standard solid-phase peptide synthesis conditions.

136 Peptide synthesis depended on sequence complementarity b

137 fostered in part by advances in large-scale peptide synthesis, development of peptides as therapeuti

138 povelutibol D was prepared using solid-phase peptide synthesis followed by an O-N acyl migration to i

139 ntacyclic regions that derive from ribosomal peptide synthesis followed by extensive posttranslationa

140 -OH (10), were synthesized using solid phase peptide synthesis followed by rhenium cyclization.

141 lding blocks are compatible with solid-phase peptide synthesis following the 9-fluorenylmethoxycarbon

142 ide carbonyl derivatives rely on solid-phase peptide synthesis for amide functionalization.

143 d that they could be utilized in solid phase peptide synthesis for the preparation of a biologically

144 per, we provide the first direct evidence of peptide synthesis from the S-adenosylmethionine decarbox

145 ere involved in noncoded thioester-dependent peptide synthesis, functionally similar to the present d

146 ere involved in noncoded thioester-dependent peptide synthesis, functionally similar to the present-d

147 Solid-phase peptide synthesis has been an attractive method for synt

148 Solid-state peptide synthesis has been applied to link the acids to

149 Solid-phase peptide synthesis has been applied to the preparation of

150 When the peptide synthesis has been completed, activation of the

151 to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological r

152 Multiple sequence analysis and peptide synthesis have identified a core set of residues

153 Molecular modeling associated with peptide synthesis improved bactericidal efficacy in addi

154 Combinatorial peptide synthesis in combination with affinity selection

155 of 100 amino acid residues using solid phase peptide synthesis in combination with native chemical li

156 as the detection method for the solid-phase peptide synthesis in combinatorial chemistry.

157 Peptide synthesis in eukaryotes terminates when eukaryot

158 ying computational simulation techniques and peptide synthesis in liquid phase.

159 IL-23 drive substance P gene expression and peptide synthesis in murine splenic T cells and macropha

160 The protecting group can be applied to peptide synthesis in solution as well as on a solid phas

161 al study of different possible mechanisms of peptide synthesis in the ribosome has been carried out u

162 n of proline, whose unique side-chain stalls peptide synthesis in vitro, also slows the ribosome in v

163 g blocks, suitably protected for solid-phase peptide synthesis, in 2-3 steps starting from inexpensiv

164 tion of microwave irradiation to solid-phase peptide synthesis increases product purity and reduces r

165 that PGRP-LC is important for antibacterial peptide synthesis induced by Escherichia coli both in vi

166 However, the current state of the art in peptide synthesis involves primarily legacy technologies

167 Nonribosomal peptide synthesis involves the interplay between covalen

168 Peptide synthesis is a truly interdisciplinary tool, fam

169 The central chemical step of peptide synthesis is amide bond formation, which is typi

170 riately protected for Fmoc-based solid-phase peptide synthesis is described.

171 embodying a credible early history for coded peptide synthesis is readily constructed based on these

172 ded from a saturated sugar-transport system; peptide synthesis is reduced under high-light, high CO(2

173 luding the Fmoc/tBu strategy for solid phase peptide synthesis, is stable under mild acidic condition

174 y different reactions, including solid-phase peptide synthesis, iterative cross-coupling and accessin

175 vel hybrid unnatural amino acids, during the peptide synthesis itself.

176 oncise assessment of the state-of-the-art in peptide synthesis look like?

177 he regulation of transcription, translation, peptide synthesis, macromolecular sequestration and traf

178 s employs protocols derived from solid-phase peptide synthesis, making the methodology straightforwar

179 A solid-phase peptide synthesis methodology that allows for the rapid

180 = 2-6) have been synthesized by solid-phase peptide synthesis methods and characterized by 1H NMR sp

181 Using solid-phase peptide synthesis methods, two units of the mannosyl der

182 obutylglycine), were prepared by solid-phase peptide synthesis methods.

183 ocell with an RNA genome, ribozyme-catalysed peptide synthesis might have been sufficient to initiate

184 nalogs at several steps along the pathway to peptide synthesis, much of the specificity resides at th

185 Ribosomes that stall before completing peptide synthesis must be recycled and returned to the c

186 e of the main techniques, namely solid phase peptide synthesis, native chemical ligation, Staudinger

187 heterogeneous reaction medium in solid-phase peptide synthesis necessitates the use of large equivale

188 al protein did not affect factor-independent peptide synthesis; nor did lethal mutations of nucleotid

189 e demonstrated functions in the nonribosomal peptide synthesis (NRPS)/polyketide (PK) synthesis or tr

190 le of mRNA translation, the process by which peptide synthesis occurs according to the genetic code t

191 ets" are synthesized by standard solid-phase peptide synthesis of a linear precursor followed by solu

192 ation of the products during the solid-phase peptide synthesis of glycine, alanine, and valine mediat

193 applied in the manual Fmoc-based solid-phase peptide synthesis of Leu-enkephalin and in microwave-ass

194 how here the first full stepwise solid phase peptide synthesis of mambalgin-1 and confirm the biologi

195 Solid-phase peptide synthesis of several flavopeptin species and der

196 rtain thioesterases involved in nonribosomal peptide synthesis of siderophores and antibiotics.

197 an be carried out as part of the solid-phase peptide synthesis, or it can be undertaken in the soluti

198 w and innovative applications in organic and peptide synthesis, polymer chemistry, material sciences,

199 ive heating occasionally has been applied to peptide synthesis, precise microwave irradiation to heat

200 ked glycopeptide was extended using standard peptide synthesis procedures.

201 esidue into peptides by standard solid-phase peptide synthesis procedures.

202 The microwave-assisted parallel peptide synthesis protocol has been used to generate a l

203 However, standard peptide synthesis protocols are not efficient enough to

204 lysine derivative can be used in solid-phase peptide synthesis, providing access to proteins that con

205 e conjugates were synthesized by solid-phase peptide synthesis, purified by reversed-phase high-perfo

206 s application is limited by the high cost of peptide synthesis, rapid proteolysis, and poor efficacy

207 rivative can be used directly in solid phase peptide synthesis, rendering bPNA+ conveniently accessib

208 rganic solvents commonly used in polymer and peptide synthesis results in the alteration of DNA helic

209 d building blocks, followed by a solid-phase peptide synthesis sequence, featuring two resin-bound ma

210 e usefulness of this strategy in solid-phase peptide synthesis, several bioactive peptides have been

211 zing coupling reagents typically employed in peptide synthesis, several different deoxyguanosine nucl

212 nes are not recommended as intermediates for peptide synthesis, since model studies showed that lacto

213 ne [Trt-Cys(Xan)-OH] has been introduced for peptide synthesis, specifically for application to a new

214 d by a combination of Fmoc-based solid-phase peptide synthesis (SPPS) and beta-hydroxyaspartic acid l

215 ues, which are incompatible with solid-phase peptide synthesis (SPPS) due to the intrinsic acid labil

216 ha-Azido acids have been used in solid phase peptide synthesis (SPPS) for almost 20 years.

217 canoic acid) required to support solid phase peptide synthesis (SPPS) for structure-activity studies

218 Solid-phase peptide synthesis (SPPS) is a widely used technique in b

219 direct attachment of biotin and solid phase peptide synthesis (SPPS) of histidine (His)- and human i

220 synthetic approach relies on the solid-phase peptide synthesis (SPPS) of N-terminal thioesters (inclu

221 They are prepared by solid-phase peptide synthesis (SPPS) or biosynthetically by protein

222 r-Ile-Asn-Gly-OH), following the solid-phase peptide synthesis (SPPS) protocol and Amyloid beta (39-4

223 The use of solid-phase peptide synthesis (SPPS) to prepare four such arrays, co

224 ementary strategies: linear Fmoc solid-phase peptide synthesis (SPPS) using several advancements for

225 Solid-phase peptide synthesis (SPPS) using tert-butyloxycarbonyl (Bo

226 are directly incorporated after solid-phase peptide synthesis (SPPS) via on-resin derivatization of

227 elator to be incorporated during solid-phase peptide synthesis (SPPS) with total site specificity.

228 building blocks during automated solid phase peptide synthesis (SPPS), followed by orthogonal deprote

229 r thiopeptide cores prepared via solid-phase peptide synthesis (SPPS), giving an efficient and modula

230 riately protected for Fmoc-based solid-phase peptide synthesis (SPPS), is described.

231 In solid phase peptide synthesis (SPPS), the most common photoremovable

232 e 20, suitable for Fmoc-strategy solid-phase peptide synthesis (SPPS), was achieved in four steps fro

233 ag, available via semi-automated solid-phase peptide synthesis (SPPS), while equipping the antibody w

234 ribe a simple and efficient Fmoc solid-phase peptide synthesis (SPPS)-based method for synthesizing d

235 compatibility with standard Fmoc solid-phase peptide synthesis (SPPS).

236 enient use as building blocks in solid phase peptide synthesis (SPPS).

237 )-fluorenylmethoxycarbonyl-based solid-phase peptide synthesis (SPPS).

238 reaction that occurs during Fmoc solid-phase peptide synthesis (SPPS).

239 cal ligation (NCL) reaction with solid phase peptide synthesis (SPPS).

240 amino acids at variable positions during the peptide synthesis step.

241 s from the crude preparations of solid-phase peptide synthesis step.

242 te amino acids are amenable to standard Fmoc peptide synthesis strategy, and the resulting compounds

243 s been developed on the basis of solid-phase peptide synthesis techniques and is accomplished through

244 orated into peptides by standard solid-phase peptide synthesis techniques.

245 High-throughput peptide synthesis technology is therefore urgently neede

246 Previously, we developed automated flow peptide synthesis technology that greatly accelerates th

247 PAGE) with peptides produced via solid-phase peptide synthesis that correspond to the TM domain of FG

248 ent study describes innovations in poly-beta-peptide synthesis that enable the preparation of diverse

249 ions, carries out a function in nonribosomal peptide synthesis that is analogous to the function of t

250 that might ultimately provide for efficient peptide synthesis that is fully reliant on enantioselect

251 To investigate one kind of template-like peptide synthesis that might emerge from an RNA world, w

252 After AQUA peptide synthesis, the development, optimization and app

253 After peptide synthesis, the Hyp protecting group is orthogona

254 After peptide synthesis, the resin bound peptide was cleaved u

255 Standard solid-phase peptide synthesis then resulted in the desired amide-lin

256 mino acids are not accommodated in ribosomal peptide synthesis, these results suggest that the same p

257 ide synthetases (NRPSs) carry out instructed peptide synthesis through a series of directed intermodu

258 ncorporated into peptides during solid-phase peptide synthesis through reductive amination.

259 hat is several-fold greater than the average peptide synthesis time in translation reactions with a f

260 ur results highlight the ability of advanced peptide synthesis to achieve SAR tractability in a chall

261 ng materials and was employed in solid-phase peptide synthesis to afford the desired cyclic peptide s

262 e building blocks for Fmoc-based solid-phase peptide synthesis to allow convenient incorporation of t

263 this reason, some may perceive even complex peptide synthesis to be a "solved problem", while others

264 lactam linkage were prepared by solid phase peptide synthesis to explore possible biologically activ

265 cal methods, taking advantage of solid-phase peptide synthesis to incorporate approximately isosteric

266 ides are synthesized by standard solid-phase peptide synthesis to incorporate Fmoc-hydroxyproline (4R

267 emolysin (alphaHL) pore by using solid-phase peptide synthesis to make the central segment of the pol

268 (N-(9-fluorenyl)methoxycarbonyl) solid-phase peptide synthesis to prepare alpha-amino-n-butyric acid

269 1-carboxylic acid (1), which will be used in peptide synthesis to prepare glycopeptides containing ca

270 ecular recognition, we have used solid-phase peptide synthesis to prepare individual ring A and B str

271 syl-carborane building blocks in solid phase peptide synthesis to produce selective boron delivery ag

272 aced with diaminopimelate during solid-phase peptide synthesis to produce several analogues.

273 According to the RNA world hypothesis, coded peptide synthesis (translation) must have been first cat

274 The method is based on solid-phase peptide synthesis using 2-chlorotrityl resin as the soli

275 stone N termini were prepared by solid phase peptide synthesis using an acid labile Boc/HF assembly s

276 ing an N-Me group during regular solid-phase peptide synthesis using Boc protection.

277 n proteolysis was synthesized by solid-phase peptide synthesis using known mixtures of natural abunda

278 Peptide synthesis using soluble, yet isolable, supports

279 conjugated to bombesin(7-14) by solid-phase peptide synthesis using standard Fmoc chemistry.

280 challenging in the past because solid-phase peptide synthesis usually starts from the C-terminus, wh

281 eselected synthetic sequence via solid phase peptide synthesis was designed to produce 2,3-diaminopro

282 Solid-phase peptide synthesis was employed to elaborate the requisit

283 oc-protected DOTAla suitable for solid phase peptide synthesis was synthesized and integrated into po

284 Peptide synthesis was used to create a fully functional

285 Peptide synthesis was used to verify the determined sequ

286 Here, using chemical peptide synthesis, we further confirmed the importance o

287 Using the SPOTs technique of peptide synthesis, we identified the sequences in Mena t

288 alogues synthesized by Fmoc/t-Bu solid phase peptide synthesis were used to analyze their enzymatic d

289 54-375 of R2 was generated using solid-phase peptide synthesis where 354, a serine in the wild-type (

290 refers to a hypothetical era prior to coded peptide synthesis, where RNA was the major structural, g

291 y similar to solid-phase oligo nucleotide or peptide synthesis, wherein the polymer is built up one u

292 ith HOAt are excellent coupling reagents for peptide synthesis which are generally superior to their

293 fully compatible with Fmoc/ tBu solid-phase peptide synthesis, which allowed for the labeling of oct

294 The peptide synthesis will typically take, overall, 3-4 h fo

295 nthesized in Boc-protected form suitable for peptide synthesis with an overall yield of 20% in 10 ste

296 ished through the combination of solid-phase peptide synthesis with detailed liquid chromatography-ma

297 ese peptides were prepared using solid-phase peptide synthesis with Fmoc alpha-amino protection.

298 ary metabolism: the coupling of nonribosomal peptide synthesis with oxidative aromatic cross-linking

299 iew of the advances in microwave heating for peptide synthesis, with a focus on systematic studies an

300 bably through the processing of a regulatory peptide synthesised within the developing cyst.