ライフサイエンスコーパス: permeabilization

1 lytic activation are necessary steps for PLB permeabilization.

2 rbazone (Dp44mT), induces lysosomal membrane permeabilization.

3 ress diminished cell death but not lysosomal permeabilization.

4 crophages (BMDMs) as an indicator of this PM permeabilization.

5 neage kinase domain-like and plasma membrane permeabilization.

6 s, notably in relationship to outer membrane permeabilization.

7 et the lysosome to induce lysosomal membrane permeabilization.

8 ti-apoptotic components and by promoting MOM permeabilization.

9 responsible for the action of NMDARs on BBB permeabilization.

10 microscopy, and methods assessing bacterial permeabilization.

11 ipidated peptide dimers exhibited strong LPS permeabilization.

12 arge BAX oligomers are not essential for the permeabilization.

13 totic stimuli and the onset of mitochondrial permeabilization.

14 th Bax, induces mitochondrial outer membrane permeabilization.

15 try and assembly pathway leading to membrane permeabilization.

16 ts dithiothreitol and tempol did not reverse permeabilization.

17 a transient electroporation and a persistent permeabilization.

18 es GzmB-induced mitochondrial outer membrane permeabilization.

19 ing to Bak homo-oligomerization and membrane permeabilization.

20 ation is important for fungal and tumor cell permeabilization.

21 e BAX-dependent mitochondrial outer membrane permeabilization.

22 ells leading to membrane destabilization and permeabilization.

23 s inefficient in both liposome insertion and permeabilization.

24 ausing curvature-induced strain and eventual permeabilization.

25 merization, and mitochondrial outer membrane permeabilization.

26 t cell rounding within 2 minutes of perforin permeabilization.

27 g mitochondrial targeting and outer-membrane permeabilization.

28 only used as a molecular device for membrane permeabilization.

29 drial swelling, depolarization, and membrane permeabilization.

30 hibitor abolished Sap 4 activity in lysosome permeabilization.

31 n its ability to translocate without bilayer permeabilization.

32 blunting the TRAIL-induced lysosome membrane permeabilization.

33 reby regulating outer mitochondrial membrane permeabilization.

34 anes and kill cells without causing membrane permeabilization.

35 ious mechanisms, including cellular membrane permeabilization.

36 ality of membrane insertion and pH-dependent permeabilization.

37 suppression of mitochondrial outer membrane permeabilization.

38 y because of two different modes of membrane permeabilization.

39 o apoptosis via outer mitochondrial membrane permeabilization.

40 tissue paper blotting is likely due to cell permeabilization.

41 es C, 3min and 10min with low degree of cell permeabilization.

42 First, we show that the permeabilization ability of p3, but not p1, is strongly

43 ency of light exposure required for membrane permeabilization activation and also decreased dark-stat

44 Permeabilization activity is completely dependent on the

45 lt of these area changes, we observe vesicle permeabilization after a time lag that is characterized

46 It also promotes phagosomal membrane permeabilization, allowing dsDNA and IgG to leak into th

47 The permeabilization also permitted immunostaining of CD38,

48 osol where it mediates mitochondria membrane permeabilization and activation of executioner caspases.

49 ins attached to membranes after fixation and permeabilization and can therefore be used in combinatio

50 neration, and are required for mitochondrial permeabilization and caspase activation in the axon.

51 F, delaying the mitochondrial outer membrane permeabilization and caspase-9 activation.

52 osemicarbazones increased lysosomal membrane permeabilization and cell death.

53 carbazone (Dp44mT) causes lysosomal membrane permeabilization and cell death.

54 Bid-induced mitochondrial membrane permeabilization and cytochrome c release are central to

55 Bak is critical for Bid-induced OMM permeabilization and cytochrome c release, and Mfn1/2(-/

56 that Silica NP exposure could increased cell permeabilization and decreased mitochondrial membrane po

57 oligomerization of defensins during membrane permeabilization and demonstrate the existence of a "pho

58 presentative analogue revealed cell membrane permeabilization and depolarization in M bovis BCG.

59 idly bactericidal compounds primarily act by permeabilization and depolarization of bacterial membran

60 This interaction induced membrane permeabilization and depolarization.

61 es undergo subsequent mitochondrial membrane permeabilization and die.

62 erved cytotoxicity, suggesting that membrane permeabilization and disintegration underpin cyclotide c

63 some administration increased tumor vascular permeabilization and drug accumulation, which was accoun

64 lipotoxicity through lysosomal-mitochondrial permeabilization and ER stress that ultimately result in

65 down of the erythrocyte cytoskeleton, before permeabilization and eventual rupture of the erythrocyte

66 evention of inflammatory endothelial barrier permeabilization and explain the underlying signaling me

67 , which is known to be required for membrane permeabilization and explains the strong inhibition by C

68 also knockout its ability to induce membrane permeabilization and fungal growth arrest.

69 al activity were membrane active and induced permeabilization and fusion of virus-like lipid vesicles

70 e FISH-Flow protocol involves cell fixation, permeabilization and hybridization with a set of fluores

71 that a complex landscape with optimal stroma permeabilization and immune cell activation is able to m

72 rgeting CD13(+) vessels, induces endothelial permeabilization and improves tumor access of cytostatic

73 om impaired energy homeostasis and lysosomal permeabilization and inflammation through the secretion

74 y sensitive to aggregation-induced bacterial permeabilization and killing.

75 sxA:B heterodimer required for EsxA membrane permeabilization and mycobacterial cytosolic translocati

76 cess driven by gasdermin D-mediated cellular permeabilization and presumed osmotic forces thought to

77 Electrosensitization facilitated membrane permeabilization and reduced survival in cell suspension

78 ective lysosomes leads to lysosomal membrane permeabilization and release of cathepsin D, which contr

79 TNF-induced necrosis resulted from lysosomal permeabilization and release of cathepsins B and L in au

80 is, as indicated by enhanced plasma membrane permeabilization and release of danger-associated molecu

81 ptor stimulation are required for lyososomal permeabilization and release of GzmB into the cytosol.

82 and PIP-containing vesicles, histones caused permeabilization and release of vesicular aqueous conten

83 Capsid permeabilization and reverse transcription are altered w

84 ed substrate ESAT-6 is required for membrane permeabilization and that a subsequent passive leakage o

85 aminoglycosides induce rapid plasma membrane permeabilization and that this nonribosomal effect can a

86 ondria through a novel mode of mitochondrial permeabilization and through Smac degradation can compet

87 to the lysosome, where it triggers membrane permeabilization and thus lysosomal cell death (LCD).

88 pH-range shift for the onset of the membrane permeabilization and translocation activity of the T dom

89 ype VII secretion system promotes phagosomal permeabilization and type I IFN production, key features

90 n Bak oligomerization, Bak-mediated membrane permeabilization and, in a cellular context, Bak-mediate

91 namely, lateral segregation that facilitates permeabilization, and at longer times, trans-bilayer fli

92 s cell enlargement, membrane alterations and permeabilization, and biofilm and vesicle formation is d

93 e Bak oligomerization, Bak-mediated membrane permeabilization, and cell death.

94 III, attenuated mitochondrial outer membrane permeabilization, and decreased reactive oxygen species

95 trophozoites, the latter producing death via permeabilization, and DNA complexing.

96 ts including acidification, nuclear envelope permeabilization, and DNA fragmentation of the nurse cel

97 activity could be monitored just 40 s after permeabilization, and five point dose-inhibition curves

98 ochondrial membrane potential, mitochondrial permeabilization, and fragmentation of the mitochondrial

99 , we developed a methodology using fixation, permeabilization, and intracellular staining coupled wit

100 or protein tagging, cope without fixation or permeabilization, and thus, enable live cell imaging in

101 Herein, we present cell fixation and permeabilization approaches as an alternative tool for v

102 Both LMP and mitochondrial membrane permeabilization are inhibited by potassium, scavenging

103 nd its oligomerization in promoting membrane permeabilization are unclear.

104 controllers of mitochondrial outer membrane permeabilization, arguably the most important step of in

105 ally involved in protection against membrane permeabilization as it provides little protection agains

106 characterized by antibacterial and membrane permeabilization assays, X-ray crystallography, and mole

107 perties: (i) little synthetic lipid membrane permeabilization at physiological pH 7 at high peptide c

108 e a hypothesis for the mechanism of membrane permeabilization based on the results featuring a loosel

109 ptor-mediated current facilitation, membrane permeabilization, blebbing, phospholipid scrambling, inf

110 NA depletion or mitochondrial outer membrane permeabilization blockage via BCL2 overexpression or BAX

111 ucible protein BNIP3 result in mitochondrial permeabilization, but impairment in autophagic removal o

112 DAMP activity involved bacterial binding and permeabilization, but the activity was unaffected by pep

113 n cellular cryoprotection and lipid membrane permeabilization, but the governing mechanisms at membra

114 ctive caspases, mitochondrial outer membrane permeabilization by Bax and Bak results in the expressio

115 that cardiolipin directly inhibits membrane permeabilization by daptomycin.

116 in, phagolysosomal trafficking, or phagosome permeabilization by Franciscella tularensis.

117 In addition, membrane permeabilization by lysosomotropic agent l-leucyl-l-leuc

118 effects, ascorbate did not prevent thrombin permeabilization by obstructing calcium influx.

119 Within the synaptic cleft, target cell permeabilization by perforin resulted in the rapid diffu

120 sm of membrane binding, oligomerization, and permeabilization by pro- and antiapoptotic Bcl-2 members

121 interaction and inhibition of mitochondrial permeabilization by pro-apoptotic BAX.

122 cs of Madine-Darby canine kidney cells after permeabilization by saponin molecules.

123 mbiguously predict the mechanism of membrane permeabilization by the peptides.

124 rm cell barcoding that does not require cell permeabilization, can be completed in 10 minutes and can

125 d mechanism of action, induced cell membrane permeabilization, can be inferred from studies that show

126 p-by-step procedures for tissue preparation, permeabilization, cardiac-tissue pretreatment and hybrid

127 ltaC21 follow similar mechanisms of membrane permeabilization characterized by the formation of prote

128 pport for the notion that lysosomal membrane permeabilization contributes to cerebellar degeneration

129 Persistent permeabilization could also be elicited by single depola

130 We used selective permeabilization coupled with immunofluorescence as well

131 r the pulse, and a long-term, or persistent, permeabilization covering the whole voltage range.

132 actin reorganization, and lysosomal membrane permeabilization, culminating in cell death.

133 We find that Bax can induce both transient permeabilization, detected by protein release, and more

134 pport BAX-dependent membrane association and permeabilization due to an inability to stabilize BAXalp

135 ed yeast mitochondria undergo inner membrane permeabilization due to PTP opening.

136 in controlling mitochondrial outer membrane permeabilization during apoptosis.

137 espite the requirement for lysosome membrane permeabilization during TRAIL-induced apoptosis, little

138 tion, we found that Chol does not affect the permeabilization effects of P1.

139 how through quantitative studies of membrane permeabilization, electron microscopy, and soft X-ray to

140 mbrane that are unmasked upon outer membrane permeabilization facilitate the autophagic destruction o

141 cedure balances conflicting requirements for permeabilization, fixation and preservation of antigenic

142 y and automated cell staining including cell permeabilization, fluorescent staining, and molecular de

143 two sequential phases: a first precursor for permeabilization, followed by a second one for molecular

144 oporation can serve as a method for membrane permeabilization for use with D-DNP in cell cultures.

145 ease of ROS and oxidation products, envelope permeabilization (for larger molecules), and metabolic i

146 pplication of short electric pulses for cell permeabilization) generates reproducible results for del

147 ter tolerance to the Ca(2+)-induced membrane permeabilization, greater ADP-phosphorylating activity a

148 l Ca(2+) overload --> mitochondrial membrane permeabilization --> secondary energy failure.

149 This permeabilization has been explained by the activation of

150 e led to perforin exocytosis and target cell permeabilization in as little as 30 seconds.

151 adiation, modelling the alteration of oxygen permeabilization in blood vessels against repeated doses

152 status controls outer mitochondrial membrane permeabilization in hepatosteatosis.

153 te as a mediator of thrombin-induced barrier permeabilization in human umbilical vein endothelial cel

154 First, we compared StnII bilayer permeabilization in the presence of Chol or oleoyl-ceram

155 activation and mitochondrial outer membrane permeabilization in vitro and promotes GBM tumor growth

156 transient overexpression system and membrane permeabilization in vitro, suggesting that the mutants a

157 urements have shown that the rate of vesicle permeabilization increases with sphingosine concentratio

158 changes observed in the Raman spectra during permeabilization indicated acyl chain disordering along

159 ell surface, even in the absence of membrane permeabilization, indicating that 324.6 recognizes an AS

160 es were reversed on saponin-induced membrane permeabilization, indicating that differences in [Na(+)]

161 Using selective permeabilization indirect immunofluorescence microscopy

162 Molecular transport after GUV permeabilization induced by multiple pulses is additive

163 P-evoked and ivermectin-potentiated membrane permeabilization induced by P2X4 pore dilation.

164 Membrane permeabilization is dominated by hexa-, hepta- and octam

165 Light-induced membrane permeabilization is enabled with liposomal inclusion of

166 Membrane permeabilization is enhanced by a positive charge at the

167 sults suggest that the threshold for vesicle permeabilization is evident even at low levels of alpha-

168 Membrane permeabilization is required for the antifungal activity

169 Both experiments support that the permeabilization is selective for the light stimulus, hi

170 parameters of the formation of pores during permeabilization is very challenging for medical applica

171 Permeabilization kinetics were affected in a reciprocal

172 of CCHFV NSs triggers mitochondrial membrane permeabilization, leading to activation of caspases, whi

173 otic pathway is mitochondrial outer membrane permeabilization, leading to formation of the apoptosome

174 er complexes that induced lysosomal membrane permeabilization (LMP) and cytotoxicity.

175 estingly, cells with endo-lysosomal membrane permeabilization (LMP) are more vulnerable to the seedin

176 Lysosomal membrane permeabilization (LMP) is a poorly understood regulator

177 resulted in Pgp-dependent lysosomal membrane permeabilization (LMP) that relied on copper (Cu) chelat

178 on of ER stress, leads to lysosomal membrane permeabilization (LMP), a sustained accumulation of cyto

179 that EAD1 causes rapid relocation, membrane permeabilization (LMP), and deacidification of lysosomes

180 released during aging via lysosomal membrane permeabilization (LMP), leading to procaspase-3 cleavage

181 ancer effect of combining lysosomal membrane permeabilization (LMP)-inducing agent N-dodecylimidazole

182 (ROS) leads to subsequent lysosomal membrane permeabilization (LMP).

183 gs suggest that mitochondrial outer membrane permeabilization may represent a valid target for boosti

184 n release, and more substantial long-lasting permeabilization, measured by the rate of oxidation of a

185 Based on these results, a curvature-driven permeabilization mechanism dependent on the interaction

186 will be useful for further analysis of AMPs' permeabilization mechanisms.

187 Here we show that phagosomal permeabilization mediated by the bacterial ESX-1 secreti

188 nanochannels induces mitochondrial membrane permeabilization (MMP), leading to depolarization, obser

189 BID) to induce mitochondrial outer membrane permeabilization (MOMP) and apoptosis and whether these

190 OMM) to promote mitochondrial outer membrane permeabilization (MOMP) and apoptosis.

191 d initiation of mitochondrial outer membrane permeabilization (MOMP) and downstream apoptosis.

192 tic Bax induces mitochondrial outer membrane permeabilization (MOMP) by forming oligomers through a l

193 on required for mitochondrial outer membrane permeabilization (MOMP) during apoptosis.

194 Mitochondrial outer membrane permeabilization (MOMP) has historically been thought to

195 stimuli require mitochondrial outer membrane permeabilization (MOMP) in order to execute cell death.

196 n subsequent to mitochondrial outer membrane permeabilization (MOMP) in several cancer cell lines.

197 Mitochondrial outer membrane permeabilization (MOMP) is a complex multistep process.

198 Mitochondrial outer membrane permeabilization (MOMP) is a core event in apoptosis sig

199 Mitochondrial outer membrane permeabilization (MOMP) is a crucial event enabling apop

200 ax/Bak-mediated mitochondrial outer membrane permeabilization (MOMP) is essential for "intrinsic" apo

201 ered by limited mitochondrial outer membrane permeabilization (MOMP) promote genomic instability that

202 and execution, mitochondrial outer membrane permeabilization (MOMP) represents one of the most funda

203 rial apoptosis, mitochondrial outer membrane permeabilization (MOMP) usually commits a cell to die.

204 d the resultant mitochondrial outer membrane permeabilization (MOMP) via BAK and BAX oligomerization,

205 Bax induces mitochondrial outer membrane permeabilization (MOMP), a critical step in apoptosis in

206 Mitochondrial outer membrane permeabilization (MOMP), a key step in the intrinsic apo

207 family regulate mitochondrial outer membrane permeabilization (MOMP), a phenomenon in which mitochond

208 l death through mitochondrial outer membrane permeabilization (MOMP), which is considered an irrevers

209 ax/Bak-mediated mitochondrial outer membrane permeabilization (MOMP), which releases death-promoting

210 is initiated by mitochondrial outer membrane permeabilization (MOMP).

211 x and eliciting mitochondrial outer membrane permeabilization (MOMP).

212 poptotic event: mitochondrial outer membrane permeabilization (MOMP).

213 factors causing mitochondrial outer-membrane permeabilization (MOMP).

214 plasma membrane bilayer is unlikely and that permeabilization necessarily involves both anionic lipid

215 ve plasma membrane and nuclear envelope (NE) permeabilization, nuclear lamin meshwork and then NE rup

216 l was able to activate StnII-induced bilayer permeabilization; OCer failed to activate it.

217 When nsEP caused a transient permeabilization of 83% of cells to propidium iodide, ce

218 ngineered in vitro model to characterize the permeabilization of adhered brain endothelial cells to l

219 Transient permeabilization of cancer cell membranes created by app

220 Membrane permeabilization of cardiac myocytes with saponin and/or

221 detailed molecular mechanisms underlying the permeabilization of cell membranes by pulsed electric fi

222 plied in combination or individually induced permeabilization of cell membranes in the tomato fractio

223 uman cell types, based on pore formation and permeabilization of cell membranes with acoustic waves.

224 regation, recombinantly produced TCPs induce permeabilization of Escherichia coli and phagocytic upta

225 netics of ultrashort, electric-field-induced permeabilization of GUVs were significantly different fr

226 f the Puma BH3 domain to induce Bak-mediated permeabilization of liposomes and mitochondria, and dete

227 ternalization of Saps 4-6 results in partial permeabilization of lysosomal membranes, measured by the

228 es requires death receptor-5 (DR5)-dependent permeabilization of lysosomal membranes.

229 This leads to controlled permeabilization of membranes, as demonstrated by the re

230 was sufficient to trigger the BAX-dependent permeabilization of mitochondria or liposomes in vitro.

231 ring freeze/thaw and correcting for variable permeabilization of mitochondrial membranes.

232 igomerization and coil formation, as well as permeabilization of PA-containing liposomes.

233 ility that proteins SP-B and SP-C induce the permeabilization of phospholipid membranes via pore form

234 circulating microbubbles inducing transient permeabilization of surrounding tissues which mediates n

235 esults in a very rapid, i.e. within minutes, permeabilization of the bacterial plasma membrane, resul

236 The permeabilization of the BBB will be controlled with, and

237 , and Chol is known to facilitate subsequent permeabilization of the bilayers by StnII.

238 extravasation into the brain parenchyma via permeabilization of the blood-brain barrier.

239 Additional permeabilization of the cell membrane after fixation ena

240 The best known among these is the permeabilization of the cell membrane to large molecules

241 Controlled permeabilization of the constituent brain endothelial ce

242 Within 30 s of permeabilization of the cytoplasmic membrane by the cati

243 lipopolysaccharide (LPS) and facilitate the permeabilization of the LPS barrier, thereby improving p

244 of the bilayer by detergents and consequent permeabilization of the membrane; and 4), transition of

245 promotion of the peptide's cytotoxicity and permeabilization of the mitochondrial membrane.

246 on the mitochondrial pathway to apoptosis is permeabilization of the mitochondrial outer membrane (MO

247 roapoptotic proteins Bax and Bak mediate the permeabilization of the mitochondrial outer membrane dur

248 Permeabilization of the mitochondrial outer membrane is

249 - and antiapoptotic members that control the permeabilization of the mitochondrial outer membrane, a

250 into mitochondria, leading to Bax-dependent permeabilization of the mitochondrial outer membrane.

251 Dependence of ion-induced permeabilization of the NPC on the pathway and mode of m

252 ult from its ability to inhibit Bax-mediated permeabilization of the outer mitochondrial membrane (OM

253 Permeabilization of the outer mitochondrial membrane is

254 oint of no return for this commitment is the permeabilization of the outer mitochondrial membrane.

255 n a macrophage infection model and show that permeabilization of the phagosomal membrane does not req

256 l plasma membrane and thereby promotes rapid permeabilization of the plasma membrane and bacterial ce

257 Necroptosis is characterized by rapid permeabilization of the plasma membrane, which is associ

258 eleased into the cytosolic compartment after permeabilization of the secretory granules.

259 abeled surface cells, causing DAPI labeling (permeabilization) of underlying cells.

260 the plasma membrane, which leads to membrane permeabilization or disruption.

261 ally mimicked by inducing lysosomal membrane permeabilization or inhibiting autophagy, and were rever

262 uadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a uni

263 und regimes (that cause tissue liquefaction, permeabilization, or mild heating) to release tumor-deri

264 ophagy is consistent with lysosomal membrane permeabilization playing a role in the pathogenesis of A

265 ytometry included energetics (ENR); membrane permeabilization (PRM); annexin V binding (ANX), and cel

266 However, fixation and permeabilization procedures required for phosphoflow oft

267 hese results suggest that cytochrome-induced permeabilization proceeds through selective interaction

268 l by autophagy, or they can undergo a lethal permeabilization process that initiates apoptosis.

269 hat the MOM lipids play active roles in this permeabilization process.

270 could possibly be involved in other membrane permeabilization processes where lipids are thought to p

271 l growth inhibition, and blocks induced cell permeabilization properties of C1-CBP-vancomycin, sugges

272 racter associated with histidines affect the permeabilization properties of p1 and p3.

273 tructural determinants essential to membrane permeabilization, remain a mechanistic mystery.

274 hanism of outer mitochondrial membrane (OMM) permeabilization remains unclear.

275 ce the discovery that mitochondrial membrane permeabilization represents a critical step in the regul

276 arated, since persistent (but not transient) permeabilization required repetitive pulse exposure.

277 bacterium remains within the phagosome, this permeabilization results in phagosomal and cytoplasmic m

278 onsistent with the reduced lysosome membrane permeabilization, shRNA knockdown of PACS-2 in Huh-7 cel

279 lar experimental systems, including membrane permeabilization, size-exclusion chromatography-based ol

280 Digitonin-permeabilization studies of SINC-GFP-transfected HeLa ce

281 ondrial targeting signals, we used selective permeabilization studies to reveal that this KAT is orie

282 with AMZ, together with evidence of membrane permeabilization, suggests that Al reacts with membrane

283 into ECS preserved tissue with only minimal permeabilization, thereby enabling correlated light micr

284 of Gram-positive pathogens, causing membrane permeabilization to ions and cell death.

285 We observed rapid membrane permeabilization to propidium iodide and ATP efflux in r

286 Membrane permeabilization via channel formation represents a seco

287 efaction via boiling histotripsy, (b) tissue permeabilization via inertial cavitation, and (c) mild (

288 f focal adhesion protein and plasma membrane permeabilization, via the activation of caspase-7, and i

289 and monocyte cells, we showed that membrane permeabilization was dependent on the presence of membra

290 bacterial activity of the pools and membrane permeabilization was investigated.

291 Cellular membrane permeabilization was monitored by a conductance increase

292 Membrane permeabilization was quantified in CHO and GH3 cells by

293 Mitochondrial outer membrane permeabilization, which is required for mtDNA release, h

294 hesion kinase and subsequent plasma membrane permeabilization, which was blocked exclusively by fumon

295 We demonstrated that direct lysosome-permeabilization with a soluble peptide, Leu-Leu-OMe, mi

296 ombination of a non-invasive approach to BBB permeabilization with a therapeutically relevant polymer

297 report that the mechanism combines membrane permeabilization with rapid metabolic changes, including

298 f exosomes upon sonication and extrusion, or permeabilization with saponin resulted in high loading e

299 methods: the incubation at room temperature, permeabilization with saponin, freeze-thaw cycles, sonic

300 ield intensity and number of pulses for safe permeabilization without significantly compromising cell